MD08G1092200.v1.1

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011720
Physical Location & Seq
Forward (+)
7691184 .. 7693311
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1092200.v1.1.491

Sequence Viewer

Length: 852 bp
ATGCTGCTTTTCGTGTTGGTCTTGTCTCTTGTGGCTCAAGCCTGCTCAGCATCTCCAACTACCCAACAACCAAACAAAAAAACAGATCAACATCTCAAAAGCATATTCGTCCTGGCCGGACAGAGTAACATGGCGGGTCGAGGTGGCGTGGTTAACGACACCACCACAGGCATTGCCACCTGGGACGGTGTTGTGCCGCCAGAGTGCCAACCCAACCCATCAGTCTTCCGGCTCAATGTGAATCTCACGTGGGTTAAAGCAAGCGAGCCTCTCCATGCCGACATTGACGTGGAGAAGACCAATGGGATTGGACCCGGCATGGCGTTTGCCAATGCGGTATTGGCCAATCACTCTAGTACCGTTGGTCGGATTGGGTTAGTCCCCTGTGCGATCGGCGGTACTAACATAACCCAGTGGAGACGTGGCTCCTTTCTGTACAACCAGATGATCAGGAGGGCCAAGGCTTCGTTGCAGAATGGTGGTTCGATCAGAGCACTTCTGTGGTATCAGGGCGAGAGCGATACAGCTAGTAGAGAAGACGCTCTGTCTTATAAAAACAGATTGGTAACTTTTTTGGGGGATGTACGTTTGGATCTGCAATCTCCTGATCTCCCAGTAATCCAGGTGGCTCTAGCATCTGGGTATGAAGCAACACTGATAGAGATAGTTAGAGAAGCCCAGCTGGGAGTCGACCTTCTAAACGTGAGAACCGTCGACGCCAAAGGTCTGCCACTGGAGCCTGATGGCTTACACCTCACTACCCCTGCCCAGGTTGAACTTGGGAAAATGTTGGCTGATGCGTATCTTCAGTTCCTGCCCAGCGCCATAAGGGCGGAGCTACATGGAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

284

Amino Acids

30.34

Weight (kDa)

6.21

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 33 - 270 1.2e-91 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccI GTMKAC 2 cut(s) 690, 714
AciI CCGC 5 cut(s) 134, 197, 335, 396, 833
AclWI GGATC 1 cut(s) 600
AcoI YGGCCR 2 cut(s) 114, 342
AcuI CTGAAG 1 cut(s) 791
AcvI CACGTG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 717
AfaI GTAC 4 cut(s) 358, 400, 437, 585
AfiI CCNNNNNNNGG 2 cut(s) 366, 769
AgsI TTSAA 1 cut(s) 776
AhdI GACNNNNNGTC 1 cut(s) 544
AjiI CACGTC 2 cut(s) 289, 422
AjnI CCWGG 4 cut(s) 111, 179, 621, 768
AleI CACNNNNGTG 1 cut(s) 499
AluBI AGCT 3 cut(s) 527, 682, 838
AluI AGCT 3 cut(s) 527, 682, 838
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 2 cut(s) 30, 412
AlwI GGATC 1 cut(s) 600
AlwNI CAGNNNCTG 1 cut(s) 814
AoxI GGCC 3 cut(s) 114, 342, 456
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 2 cut(s) 311, 456
AsuC2I CCSGG 1 cut(s) 315
AvaII GGWCC 1 cut(s) 311
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BalI TGGCCA 1 cut(s) 344
BbrPI CACGTG 1 cut(s) 249
BbsI GAAGAC 3 cut(s) 217, 302, 543
Bbv12I GWGCWC 1 cut(s) 496
BccI CCATC 2 cut(s) 226, 737
BciT130I CCWGG 4 cut(s) 113, 181, 623, 770
BclI TGATCA 1 cut(s) 447
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 30, 412
BfaI CTAG 4 cut(s) 354, 528, 632, 850
BfoI RGCGCY 1 cut(s) 825
BglI GCCNNNNNGGC 1 cut(s) 830
BisI GCNGC 2 cut(s) 5, 197
BlpI GCTNAGC 1 cut(s) 46
BlsI GCNGC 2 cut(s) 6, 198
Bme1390I CCNGG 5 cut(s) 113, 181, 315, 623, 770
Bme18I GGWCC 1 cut(s) 311
BmeRI GACNNNNNGTC 1 cut(s) 544
BmgBI CACGTC 2 cut(s) 289, 422
BmgT120I GGNCC 2 cut(s) 311, 456
BmiI GGNNCC 3 cut(s) 313, 427, 738
BmrFI CCNGG 5 cut(s) 113, 181, 315, 623, 770
BmrI ACTGGG 2 cut(s) 406, 608
BmsI GCATC 3 cut(s) 59, 644, 787
BmuI ACTGGG 2 cut(s) 406, 608
BpiI GAAGAC 3 cut(s) 217, 302, 543
BpmI CTGGAG 1 cut(s) 755
Bpu1102I GCTNAGC 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 315
BsaAI YACGTR 1 cut(s) 249
BsaBI GATNNNNATC 2 cut(s) 90, 801
BsaHI GRCGYC 1 cut(s) 717
BsaJI CCNNGG 3 cut(s) 180, 459, 768
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 769
Bse1I ACTGG 3 cut(s) 412, 614, 738
Bse3DI GCAATG 1 cut(s) 171
Bse8I GATNNNNATC 2 cut(s) 90, 801
BseBI CCWGG 4 cut(s) 113, 181, 623, 770
BseDI CCNNGG 3 cut(s) 180, 459, 768
BseGI GGATG 1 cut(s) 586
BseJI GATNNNNATC 2 cut(s) 90, 801
BseLI CCNNNNNNNGG 2 cut(s) 366, 769
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 3 cut(s) 412, 614, 738
BseYI CCCAGC 3 cut(s) 678, 682, 818
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 3 cut(s) 116, 344, 458
BsiEI CGRYCG 1 cut(s) 393
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 3 cut(s) 117, 229, 315
BslFI GGGAC 2 cut(s) 197, 365
BslI CCNNNNNNNGG 2 cut(s) 366, 769
BsmAI GTCTC 2 cut(s) 30, 412
BsmBI CGTCTC 1 cut(s) 412
BsmFI GGGAC 2 cut(s) 197, 365
BsnI GGCC 3 cut(s) 116, 344, 458
Bsp1286I GDGCHC 1 cut(s) 496
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 6 cut(s) 85, 390, 447, 486, 592, 607
Bsp1720I GCTNAGC 1 cut(s) 46
BspACI CCGC 5 cut(s) 134, 197, 335, 396, 833
BspANI GGCC 3 cut(s) 116, 344, 458
BspCNI CTCAG 1 cut(s) 59
BspLI GGNNCC 3 cut(s) 313, 427, 738
BspPI GGATC 1 cut(s) 600
BsrDI GCAATG 1 cut(s) 171
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 3 cut(s) 412, 614, 738
BssECI CCNNGG 3 cut(s) 180, 459, 768
BssMI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
BssNI GRCGYC 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 4 cut(s) 113, 181, 623, 770
Bst4CI ACNGT 3 cut(s) 188, 361, 712
BstACI GRCGYC 1 cut(s) 717
BstAUI TGTACA 1 cut(s) 435
BstBAI YACGTR 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 43, 262, 266
BstDEI CTNAG 1 cut(s) 46
BstF5I GGATG 1 cut(s) 586
BstH2I RGCGCY 1 cut(s) 825
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 6 cut(s) 88, 393, 450, 489, 595, 610
BstMAI GTCTC 2 cut(s) 30, 412
BstMBI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 4 cut(s) 47, 341, 736, 830
BstNI CCWGG 4 cut(s) 113, 181, 623, 770
BstSCI CCNGG 5 cut(s) 111, 179, 313, 621, 768
BstV2I GAAGAC 3 cut(s) 217, 302, 543
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
BsuRI GGCC 3 cut(s) 116, 344, 458
BtrI CACGTC 2 cut(s) 289, 422
BtsCI GGATG 1 cut(s) 586
BtsIMutI CAGTG 3 cut(s) 419, 653, 731
Cac8I GCNNGC 3 cut(s) 43, 262, 266
CaiI CAGNNNCTG 1 cut(s) 814
CfoI GCGC 1 cut(s) 824
Cfr13I GGNCC 2 cut(s) 311, 456
CseI GACGC 2 cut(s) 548, 725
Csp6I GTAC 4 cut(s) 357, 399, 436, 584
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviAII CATG 4 cut(s) 130, 275, 319, 842
CviQI GTAC 4 cut(s) 357, 399, 436, 584
DdeI CTNAG 1 cut(s) 46
DpnI GATC 6 cut(s) 87, 392, 449, 488, 594, 609
DpnII GATC 6 cut(s) 85, 390, 447, 486, 592, 607
DriI GACNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 2 cut(s) 114, 342
Eam1105I GACNNNNNGTC 1 cut(s) 544
EciI GGCGGA 1 cut(s) 848
Eco130I CCWWGG 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 791
Eco72I CACGTG 1 cut(s) 249
EcoRII CCWGG 4 cut(s) 111, 179, 621, 768
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 412
FaeI CATG 4 cut(s) 133, 278, 322, 845
FaiI YATR 9 cut(s) 104, 131, 276, 320, 407, 552, 645, 827, 843
FaqI GGGAC 2 cut(s) 197, 365
FatI CATG 4 cut(s) 129, 274, 318, 841
FauI CCCGC 1 cut(s) 127
FbaI TGATCA 1 cut(s) 447
FblI GTMKAC 2 cut(s) 690, 714
Fnu4HI GCNGC 2 cut(s) 5, 197
FokI GGATG 1 cut(s) 593
Fsp4HI GCNGC 2 cut(s) 5, 197
FspBI CTAG 4 cut(s) 354, 528, 632, 850
GlaI GCGC 1 cut(s) 823
GluI GCNGC 2 cut(s) 5, 197
GsaI CCCAGC 3 cut(s) 682, 686, 822
GsuI CTGGAG 1 cut(s) 755
HaeII RGCGCY 1 cut(s) 825
HaeIII GGCC 3 cut(s) 116, 344, 458
HapII CCGG 3 cut(s) 117, 229, 315
HgaI GACGC 2 cut(s) 548, 725
HhaI GCGC 1 cut(s) 824
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 4 cut(s) 133, 278, 322, 845
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 3 cut(s) 154, 691, 715
HindII GTYRAC 3 cut(s) 154, 691, 715
HinfI GANTC 2 cut(s) 241, 687
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 3 cut(s) 117, 229, 315
Hpy166II GTNNAC 3 cut(s) 154, 691, 715
Hpy188I TCNGA 2 cut(s) 369, 491
Hpy188III TCNNGA 2 cut(s) 451, 605
Hpy8I GTNNAC 3 cut(s) 154, 691, 715
Hpy99I CGWCG 2 cut(s) 716, 719
HpyAV CCTTC 1 cut(s) 704
HpyCH4III ACNGT 3 cut(s) 188, 361, 712
HpyCH4IV ACGT 5 cut(s) 248, 288, 421, 586, 702
HpyCH4V TGCA 2 cut(s) 472, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 341, 736, 830
HpyF3I CTNAG 1 cut(s) 46
HpySE526I ACGT 5 cut(s) 248, 288, 421, 586, 702
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 4 cut(s) 133, 278, 322, 845
HspAI GCGC 1 cut(s) 822
Ksp22I TGATCA 1 cut(s) 447
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 6 cut(s) 85, 390, 447, 486, 592, 607
LmnI GCTCC 3 cut(s) 431, 736, 835
LweI GCATC 3 cut(s) 59, 644, 787
MaeI CTAG 4 cut(s) 354, 528, 632, 850
MaeII ACGT 5 cut(s) 248, 288, 421, 586, 702
MaeIII GTNAC 2 cut(s) 125, 565
MalI GATC 6 cut(s) 87, 392, 449, 488, 594, 609
MboI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
MboII GAAGA 4 cut(s) 217, 307, 548, 797
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 1 cut(s) 496
MlsI TGGCCA 1 cut(s) 344
MluNI TGGCCA 1 cut(s) 344
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 2 cut(s) 80, 347
MnlI CCTC 5 cut(s) 134, 279, 447, 764, 839
Mox20I TGGCCA 1 cut(s) 344
MscI TGGCCA 1 cut(s) 344
MseI TTAA 2 cut(s) 153, 255
MslI CAYNNNNRTG 2 cut(s) 287, 499
Msp20I TGGCCA 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 682
MspI CCGG 3 cut(s) 117, 229, 315
MspR9I CCNGG 5 cut(s) 113, 181, 315, 623, 770
MvaI CCWGG 4 cut(s) 113, 181, 623, 770
MwoI GCNNNNNNNGC 4 cut(s) 47, 341, 736, 830
NciI CCSGG 1 cut(s) 315
NdeII GATC 6 cut(s) 85, 390, 447, 486, 592, 607
NlaIII CATG 4 cut(s) 133, 278, 322, 845
NlaIV GGNNCC 3 cut(s) 313, 427, 738
OliI CACNNNNGTG 1 cut(s) 499
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 2 cut(s) 6, 198
Ple19I CGATCG 1 cut(s) 393
PleI GAGTC 1 cut(s) 695
PmaCI CACGTG 1 cut(s) 249
PmlI CACGTG 1 cut(s) 249
PpsI GAGTC 1 cut(s) 695
Ppu21I YACGTR 1 cut(s) 249
PsiI TTATAA 1 cut(s) 552
Psp6I CCWGG 4 cut(s) 111, 179, 621, 768
PspCI CACGTG 1 cut(s) 249
PspFI CCCAGC 3 cut(s) 678, 682, 818
PspGI CCWGG 4 cut(s) 111, 179, 621, 768
PspN4I GGNNCC 3 cut(s) 313, 427, 738
PspPI GGNCC 2 cut(s) 311, 456
PsrI GAACNNNNNNTAC 2 cut(s) 794, 826
PstNI CAGNNNCTG 1 cut(s) 814
PsuI RGATCY 1 cut(s) 592
PvuI CGATCG 1 cut(s) 393
PvuII CAGCTG 1 cut(s) 682
RsaI GTAC 4 cut(s) 358, 400, 437, 585
RsaNI GTAC 4 cut(s) 357, 399, 436, 584
RseI CAYNNNNRTG 2 cut(s) 287, 499
SalI GTCGAC 2 cut(s) 689, 713
SaqAI TTAA 2 cut(s) 153, 255
SatI GCNGC 2 cut(s) 5, 197
Sau3AI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
Sau96I GGNCC 2 cut(s) 311, 456
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 5 cut(s) 113, 181, 315, 623, 770
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 3 cut(s) 59, 644, 787
SgrDI CGTCGACG 1 cut(s) 713
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 2 cut(s) 287, 499
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
SsiI CCGC 5 cut(s) 134, 197, 335, 396, 833
SspMI CTAG 4 cut(s) 354, 528, 632, 850
StyD4I CCNGG 5 cut(s) 111, 179, 313, 621, 768
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 3 cut(s) 188, 361, 712
TaiI ACGT 5 cut(s) 251, 291, 424, 589, 705
TaqI TCGA 4 cut(s) 139, 485, 690, 714
TatI WGTACW 1 cut(s) 435
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 2 cut(s) 153, 255
Tru9I TTAA 2 cut(s) 153, 255
TscAI CASTG 3 cut(s) 419, 660, 738
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 660
TspRI CASTG 3 cut(s) 419, 660, 738
VpaK11BI GGWCC 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 3 cut(s) 337, 419, 776
XmiI GTMKAC 2 cut(s) 690, 714
XspI CTAG 4 cut(s) 354, 528, 632, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.