Rroxscaffold_7G00159960

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011720
Physical Location & Seq
Reverse (-)
2987680 .. 2995194
7515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159960.1

Sequence Viewer

Length: 1386 bp
ATGATGATGCTTTTCGTGTTGTTCTTGTCTCTTGCGTCTCACGCCTCCTCATCAAAAGAACAACACATCAAAAGCATTTTCATCCTGGCCGGACAGAGCAACATGGCCGGCCGAGGAGGCGTTGTAAACGACACTCTCACCGGAATCACCACCTGGGACGGCGTCGTTCCTCCAGAGTGCCTCCCCAACAAATCCATCTTCCGACTCAGCGCAAACCTCACGTGGGTTGAAGCCCGCGAGCCTCTCCACGCTGATATCGACGTGGAGAAGACGAATGGGATCGGACCCGGGATGCCATTTGCCAGTGCGATCCTTGCCAATCGTGCTAGTAGCATTGGTGCGATTGGTTTAGTCCCGTGCGCAATCGGCGGGACTAACCTAAGCCAATGGGAACGAGGGTCTTTCCTATACAGACAGATGATTGGGAGAGCCAAGGCGGCTTTGTTAAAGGTGAACGATAATGGCGCCTCAATCAGGGGACTACTCTGGTATCAAGGTGAGAGCGATACGACTAGTATAGAAGACGCCCGATCTTACAAAGCCAGATTGGAAACCTTCTTTGGAGACCTCCGGTTGGATCTGCAATTTCCTATGCTTCCAATAATCCAGGTTGCAATTGCATCTGGGTCAGGACCATACATAGAGATCATTAGAGAAGCTCAGATGGAAATTGACCTGCTAAACGTGCGAACCGTTGATGCCAAGGGTCTAACATTAGAACCTGATAGGTTGCACCTCACTACCCCGAGTCAGGCCTCCAAAATGTCAGGACCAACCCTGGGAAGAGGCAGCGTCAACGGCCGCAAGTGGGACGGCAACGTTCCTCCCGAGTGCACACCGACCCCGTCAATTCTCAGACTGACTGCTCAACTCGTTTGGGAAGAAGCACGAGAGCCACTCCACGCCGACATTGATGTGGGCAAGGGCAAGACGTGTGGGGTTGGGCCAGGGATGGCTTTTGCCAACAAGGTAGCACTGGCATCAGGAGAAGGTCAGTTCATAGATATAGTGAGGAAAGCACAGCTGGAAACGAAGCTGCCTAATGTGAAATGTGTCGATGCTAAAGGATTGCCTCTGAAACCGGATAATCTCCACCTCACTACCATATCGGAGGGAACTGAAGGTGTAAACGAGCAAGTTGGAGTGGAGACCAAGGTGGAGACAGTGGATTTCCGATCACCGGCAGGAGAAGACAAAGAACCCGTAAAGGAGAAGGTGGAAATACTTCATGAGATCAACGACGGAGGAGAAAATTCTGGAAATACCGGGGCTGCCGGCTTTGTGGGTAAAGCAGCTATTGCAGTTGCAGATACCTTTCAGTCAGCCAAGGATGCTGTTTCTGGGAAACCCAAGGATGCTGTTGCTGAAAATAAGACCACGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

461

Amino Acids

49.21

Weight (kDa)

5.67

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 24 - 252 2.3e-83 Carbohydrate esterase, sialic acid-specific acetylesterase
SASA PF03629 261 - 325 9.8e-19 Carbohydrate esterase, sialic acid-specific acetylesterase
SASA PF03629 324 - 372 1.7e-09 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 361
Acc36I ACCTGC 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 1 cut(s) 237
AciI CCGC 4 cut(s) 235, 369, 437, 802
AclI AACGTT 1 cut(s) 819
AclWI GGATC 3 cut(s) 287, 304, 585
AcoI YGGCCR 4 cut(s) 87, 105, 109, 799
AcsI RAATTY 1 cut(s) 1252
AcuI CTGAAG 1 cut(s) 1140
AcvI CACGTG 1 cut(s) 222
AcyI GRCGYC 3 cut(s) 162, 465, 525
AfiI CCNNNNNNNGG 7 cut(s) 223, 474, 574, 751, 779, 808, 1180
AflIII ACRYGT 1 cut(s) 932
AgsI TTSAA 1 cut(s) 230
AhlI ACTAGT 1 cut(s) 512
AjiI CACGTC 2 cut(s) 262, 933
AjnI CCWGG 5 cut(s) 84, 152, 606, 777, 946
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AluBI AGCT 4 cut(s) 659, 1024, 1036, 1295
AluI AGCT 4 cut(s) 659, 1024, 1036, 1295
Alw21I GWGCWC 1 cut(s) 836
Alw26I GTCTC 5 cut(s) 33, 42, 558, 1142, 1154
Alw44I GTGCAC 1 cut(s) 832
AlwI GGATC 3 cut(s) 287, 304, 585
Ama87I CYCGRG 3 cut(s) 287, 745, 827
AoxI GGCC 6 cut(s) 87, 105, 109, 753, 799, 944
ApaLI GTGCAC 1 cut(s) 832
ApeKI GCWGC 4 cut(s) 789, 1036, 1271, 1292
ApoI RAATTY 1 cut(s) 1252
Asp700I GAANNNNTTC 1 cut(s) 1224
AspLEI GCGC 3 cut(s) 212, 362, 467
AspS9I GGNCC 4 cut(s) 284, 632, 770, 944
AsuC2I CCSGG 3 cut(s) 288, 289, 1267
AsuHPI GGTGA 5 cut(s) 130, 139, 463, 509, 1170
AvaI CYCGRG 3 cut(s) 287, 745, 827
AvaII GGWCC 3 cut(s) 284, 632, 770
BaeGI GKGCMC 1 cut(s) 836
BaeI ACNNNNGTAYC 2 cut(s) 473, 506
BanI GGYRCC 1 cut(s) 464
BauI CACGAG 1 cut(s) 888
BbrPI CACGTG 1 cut(s) 222
BbsI GAAGAC 3 cut(s) 275, 528, 1197
Bbv12I GWGCWC 1 cut(s) 836
BbvI GCAGC 4 cut(s) 801, 1023, 1258, 1304
BccI CCATC 3 cut(s) 203, 658, 946
BceAI ACGGC 3 cut(s) 175, 814, 829
BciT130I CCWGG 5 cut(s) 86, 154, 608, 779, 948
BcnI CCSGG 3 cut(s) 288, 289, 1267
BcoDI GTCTC 5 cut(s) 33, 42, 558, 1142, 1154
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 2 cut(s) 327, 513
BfoI RGCGCY 1 cut(s) 468
BfuAI ACCTGC 1 cut(s) 684
BglI GCCNNNNNGGC 2 cut(s) 117, 437
BisI GCNGC 6 cut(s) 438, 790, 802, 1037, 1272, 1293
BlsI GCNGC 6 cut(s) 439, 791, 803, 1038, 1273, 1294
Bme1390I CCNGG 8 cut(s) 86, 154, 288, 289, 608, 779, 948, 1267
Bme18I GGWCC 3 cut(s) 284, 632, 770
BmeT110I CYCGRG 3 cut(s) 287, 745, 827
BmgBI CACGTC 2 cut(s) 262, 933
BmgT120I GGNCC 4 cut(s) 284, 632, 770, 944
BmiI GGNNCC 2 cut(s) 286, 466
BmrFI CCNGG 8 cut(s) 86, 154, 288, 289, 608, 779, 948, 1267
BmsI GCATC 7 cut(s) 282, 629, 688, 989, 1048, 1321, 1345
BpiI GAAGAC 3 cut(s) 275, 528, 1197
BplI GAGNNNNNCTC 2 cut(s) 882, 914
BpmI CTGGAG 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 380
BpuMI CCSGG 3 cut(s) 288, 289, 1267
BsaAI YACGTR 1 cut(s) 222
BsaHI GRCGYC 3 cut(s) 162, 465, 525
BsaI GGTCTC 2 cut(s) 558, 1142
BsaWI WCCGGW 3 cut(s) 140, 570, 1081
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 7 cut(s) 223, 474, 574, 751, 779, 808, 1180
Bse118I RCCGGY 3 cut(s) 107, 1180, 1274
Bse1I ACTGG 2 cut(s) 303, 981
BseBI CCWGG 5 cut(s) 86, 154, 608, 779, 948
BseGI GGATG 5 cut(s) 81, 297, 957, 1336, 1360
BseLI CCNNNNNNNGG 7 cut(s) 223, 474, 574, 751, 779, 808, 1180
BseMII CTCAG 3 cut(s) 220, 674, 868
BseNI ACTGG 2 cut(s) 303, 981
BseRI GAGGAG 3 cut(s) 37, 129, 1260
BseSI GKGCMC 1 cut(s) 836
BseX3I CGGCCG 2 cut(s) 109, 799
BseXI GCAGC 4 cut(s) 801, 1023, 1258, 1304
Bsh1236I CGCG 1 cut(s) 237
Bsh1285I CGRYCG 2 cut(s) 112, 802
BshFI GGCC 6 cut(s) 89, 107, 111, 755, 801, 946
BshNI GGYRCC 1 cut(s) 464
BsiEI CGRYCG 2 cut(s) 112, 802
BsiHKAI GWGCWC 1 cut(s) 836
BsiHKCI CYCGRG 3 cut(s) 287, 745, 827
BsiSI CCGG 9 cut(s) 90, 108, 141, 288, 571, 1082, 1181, 1266, 1275
BslFI GGGAC 5 cut(s) 170, 338, 385, 492, 824
BslI CCNNNNNNNGG 7 cut(s) 223, 474, 574, 751, 779, 808, 1180
BsmAI GTCTC 5 cut(s) 33, 42, 558, 1142, 1154
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 5 cut(s) 170, 338, 385, 492, 824
BsnI GGCC 6 cut(s) 89, 107, 111, 755, 801, 946
Bso31I GGTCTC 2 cut(s) 558, 1142
BsoBI CYCGRG 3 cut(s) 287, 745, 827
Bsp1286I GDGCHC 1 cut(s) 836
Bsp143I GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
BspACI CCGC 4 cut(s) 235, 369, 437, 802
BspANI GGCC 6 cut(s) 89, 107, 111, 755, 801, 946
BspCNI CTCAG 3 cut(s) 219, 673, 867
BspFNI CGCG 1 cut(s) 237
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 2 cut(s) 286, 466
BspMI ACCTGC 1 cut(s) 684
BspPI GGATC 3 cut(s) 287, 304, 585
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 2 cut(s) 558, 1142
BsrFI RCCGGY 3 cut(s) 107, 1180, 1274
BsrI ACTGG 2 cut(s) 303, 981
BssAI RCCGGY 3 cut(s) 107, 1180, 1274
BssMI GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
BssNI GRCGYC 3 cut(s) 162, 465, 525
BssSI CACGAG 1 cut(s) 888
BssT1I CCWWGG 5 cut(s) 432, 702, 1152, 1326, 1350
Bst2BI CACGAG 1 cut(s) 888
Bst2UI CCWGG 5 cut(s) 86, 154, 608, 779, 948
Bst4CI ACNGT 2 cut(s) 694, 1165
Bst6I CTCTTC 1 cut(s) 778
BstACI GRCGYC 3 cut(s) 162, 465, 525
BstAPI GCANNNNNTGC 1 cut(s) 1298
BstBAI YACGTR 1 cut(s) 222
BstC8I GCNNGC 4 cut(s) 109, 235, 239, 1276
BstDEI CTNAG 4 cut(s) 206, 380, 660, 854
BstENI CCTNNNNNAGG 1 cut(s) 472
BstF5I GGATG 5 cut(s) 81, 297, 957, 1336, 1360
BstFNI CGCG 1 cut(s) 237
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 3 cut(s) 212, 362, 467
BstKTI GATC 7 cut(s) 282, 312, 533, 580, 648, 1178, 1236
BstMAI GTCTC 5 cut(s) 33, 42, 558, 1142, 1154
BstMBI GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
BstMCI CGRYCG 2 cut(s) 112, 802
BstNI CCWGG 5 cut(s) 86, 154, 608, 779, 948
BstSCI CCNGG 8 cut(s) 84, 152, 286, 287, 606, 777, 946, 1265
BstSLI GKGCMC 1 cut(s) 836
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 4 cut(s) 801, 1023, 1258, 1304
BstV2I GAAGAC 3 cut(s) 275, 528, 1197
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BstZI CGGCCG 2 cut(s) 109, 799
BsuRI GGCC 6 cut(s) 89, 107, 111, 755, 801, 946
BtrI CACGTC 2 cut(s) 262, 933
BtsCI GGATG 5 cut(s) 81, 297, 957, 1336, 1360
BtsIMutI CAGTG 3 cut(s) 310, 974, 1170
BveI ACCTGC 1 cut(s) 684
Cac8I GCNNGC 4 cut(s) 109, 235, 239, 1276
CciI TCATGA 1 cut(s) 1228
CfoI GCGC 3 cut(s) 212, 362, 467
Cfr10I RCCGGY 3 cut(s) 107, 1180, 1274
Cfr13I GGNCC 4 cut(s) 284, 632, 770, 944
Cfr9I CCCGGG 1 cut(s) 287
CseI GACGC 4 cut(s) 24, 151, 533, 781
CspCI CAANNNNNGTGG 2 cut(s) 916, 951
CviAII CATG 2 cut(s) 103, 1229
DdeI CTNAG 4 cut(s) 206, 380, 660, 854
DinI GGCGCC 1 cut(s) 466
DpnI GATC 7 cut(s) 281, 311, 532, 579, 647, 1177, 1235
DpnII GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
EaeI YGGCCR 4 cut(s) 87, 105, 109, 799
EagI CGGCCG 2 cut(s) 109, 799
Eam1104I CTCTTC 1 cut(s) 778
EarI CTCTTC 1 cut(s) 778
EclXI CGGCCG 2 cut(s) 109, 799
Eco130I CCWWGG 5 cut(s) 432, 702, 1152, 1326, 1350
Eco147I AGGCCT 1 cut(s) 755
Eco31I GGTCTC 2 cut(s) 558, 1142
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 3 cut(s) 284, 632, 770
Eco52I CGGCCG 2 cut(s) 109, 799
Eco57I CTGAAG 1 cut(s) 1140
Eco72I CACGTG 1 cut(s) 222
Eco88I CYCGRG 3 cut(s) 287, 745, 827
EcoNI CCTNNNNNAGG 1 cut(s) 472
EcoRII CCWGG 5 cut(s) 84, 152, 606, 777, 946
EcoRV GATATC 1 cut(s) 256
EcoT14I CCWWGG 5 cut(s) 432, 702, 1152, 1326, 1350
EgeI GGCGCC 1 cut(s) 466
EheI GGCGCC 1 cut(s) 466
ErhI CCWWGG 5 cut(s) 432, 702, 1152, 1326, 1350
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 2 cut(s) 106, 1232
FaqI GGGAC 5 cut(s) 170, 338, 385, 492, 824
FatI CATG 2 cut(s) 102, 1228
FauI CCCGC 2 cut(s) 242, 362
Fnu4HI GCNGC 6 cut(s) 438, 790, 802, 1037, 1272, 1293
FokI GGATG 5 cut(s) 68, 304, 964, 1343, 1367
FseI GGCCGGCC 1 cut(s) 111
Fsp4HI GCNGC 6 cut(s) 438, 790, 802, 1037, 1272, 1293
FspBI CTAG 2 cut(s) 327, 513
FspI TGCGCA 1 cut(s) 361
GlaI GCGC 3 cut(s) 211, 361, 466
GluI GCNGC 6 cut(s) 438, 790, 802, 1037, 1272, 1293
GsuI CTGGAG 1 cut(s) 156
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 6 cut(s) 89, 107, 111, 755, 801, 946
HapII CCGG 9 cut(s) 90, 108, 141, 288, 571, 1082, 1181, 1266, 1275
HgaI GACGC 4 cut(s) 24, 151, 533, 781
HhaI GCGC 3 cut(s) 212, 362, 467
Hin1I GRCGYC 3 cut(s) 162, 465, 525
Hin1II CATG 2 cut(s) 106, 1232
Hin6I GCGC 3 cut(s) 210, 360, 465
HinP1I GCGC 3 cut(s) 210, 360, 465
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 3 cut(s) 144, 204, 748
HpaII CCGG 9 cut(s) 90, 108, 141, 288, 571, 1082, 1181, 1266, 1275
HphI GGTGA 5 cut(s) 130, 139, 463, 509, 1170
Hpy166II GTNNAC 5 cut(s) 127, 454, 796, 834, 1129
Hpy188I TCNGA 7 cut(s) 203, 284, 663, 857, 1077, 1111, 1175
Hpy188III TCNNGA 7 cut(s) 173, 630, 768, 827, 984, 1229, 1257
Hpy8I GTNNAC 5 cut(s) 127, 454, 796, 834, 1129
Hpy99I CGWCG 3 cut(s) 167, 263, 1244
HpyAV CCTTC 4 cut(s) 565, 983, 1115, 1207
HpyCH4III ACNGT 2 cut(s) 694, 1165
HpyCH4IV ACGT 5 cut(s) 221, 261, 684, 819, 932
HpyCH4V TGCA 7 cut(s) 583, 614, 620, 733, 834, 1301, 1307
HpyF3I CTNAG 4 cut(s) 206, 380, 660, 854
HpySE526I ACGT 5 cut(s) 221, 261, 684, 819, 932
Hsp92I GRCGYC 3 cut(s) 162, 465, 525
Hsp92II CATG 2 cut(s) 106, 1232
HspAI GCGC 3 cut(s) 210, 360, 465
KasI GGCGCC 1 cut(s) 464
KroI GCCGGC 2 cut(s) 107, 1274
KroNI GCCGGC 2 cut(s) 109, 1276
Kzo9I GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
Lsp1109I GCAGC 4 cut(s) 801, 1023, 1258, 1304
LweI GCATC 7 cut(s) 282, 629, 688, 989, 1048, 1321, 1345
MaeI CTAG 2 cut(s) 327, 513
MaeII ACGT 5 cut(s) 221, 261, 684, 819, 932
MalI GATC 7 cut(s) 281, 311, 532, 579, 647, 1177, 1235
MboI GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
MboII GAAGA 6 cut(s) 190, 280, 533, 795, 893, 1202
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 836
MluCI AATT 5 cut(s) 584, 615, 669, 849, 1252
Mly113I GGCGCC 1 cut(s) 465
MlyI GAGTC 2 cut(s) 198, 757
MmeI TCCRAC 3 cut(s) 226, 555, 1120
MroNI GCCGGC 2 cut(s) 107, 1274
MroXI GAANNNNTTC 1 cut(s) 1224
MseI TTAA 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 914
MspA1I CMGCKG 1 cut(s) 1024
MspI CCGG 9 cut(s) 90, 108, 141, 288, 571, 1082, 1181, 1266, 1275
MspR9I CCNGG 8 cut(s) 86, 154, 288, 289, 608, 779, 948, 1267
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 5 cut(s) 86, 154, 608, 779, 948
MvnI CGCG 1 cut(s) 237
NaeI GCCGGC 2 cut(s) 109, 1276
NarI GGCGCC 1 cut(s) 465
NciI CCSGG 3 cut(s) 288, 289, 1267
NdeII GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
NgoMIV GCCGGC 2 cut(s) 107, 1274
NlaIII CATG 2 cut(s) 106, 1232
NlaIV GGNNCC 2 cut(s) 286, 466
NmeAIII GCCGAG 1 cut(s) 137
NsbI TGCGCA 1 cut(s) 361
PagI TCATGA 1 cut(s) 1228
PasI CCCWGGG 1 cut(s) 778
PceI AGGCCT 1 cut(s) 755
PcsI WCGNNNNNNNCGW 3 cut(s) 255, 690, 825
PdiI GCCGGC 2 cut(s) 109, 1276
PdmI GAANNNNTTC 1 cut(s) 1224
PfeI GAWTC 1 cut(s) 144
PflFI GACNNNGTC 2 cut(s) 161, 844
PkrI GCNGC 6 cut(s) 439, 791, 803, 1038, 1273, 1294
PleI GAGTC 2 cut(s) 198, 756
PluTI GGCGCC 1 cut(s) 468
PmaCI CACGTG 1 cut(s) 222
PmlI CACGTG 1 cut(s) 222
PpsI GAGTC 2 cut(s) 198, 756
Ppu21I YACGTR 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 819
Psp6I CCWGG 5 cut(s) 84, 152, 606, 777, 946
PspCI CACGTG 1 cut(s) 222
PspGI CCWGG 5 cut(s) 84, 152, 606, 777, 946
PspN4I GGNNCC 2 cut(s) 286, 466
PspPI GGNCC 4 cut(s) 284, 632, 770, 944
PsuI RGATCY 1 cut(s) 577
PsyI GACNNNGTC 2 cut(s) 161, 844
PvuII CAGCTG 1 cut(s) 1024
RigI GGCCGGCC 1 cut(s) 111
RseI CAYNNNNRTG 1 cut(s) 914
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 6 cut(s) 438, 790, 802, 1037, 1272, 1293
Sau3AI GATC 7 cut(s) 279, 309, 530, 577, 645, 1175, 1233
Sau96I GGNCC 4 cut(s) 284, 632, 770, 944
SchI GAGTC 2 cut(s) 198, 757
ScrFI CCNGG 8 cut(s) 86, 154, 288, 289, 608, 779, 948, 1267
SduI GDGCHC 1 cut(s) 836
SfaNI GCATC 7 cut(s) 282, 629, 688, 989, 1048, 1321, 1345
SfoI GGCGCC 1 cut(s) 466
SinI GGWCC 3 cut(s) 284, 632, 770
SmaI CCCGGG 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 914
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 5 cut(s) 584, 615, 669, 849, 1252
SseBI AGGCCT 1 cut(s) 755
SsiI CCGC 4 cut(s) 235, 369, 437, 802
SspDI GGCGCC 1 cut(s) 464
SspMI CTAG 2 cut(s) 327, 513
StuI AGGCCT 1 cut(s) 755
StyD4I CCNGG 8 cut(s) 84, 152, 286, 287, 606, 777, 946, 1265
StyI CCWWGG 5 cut(s) 432, 702, 1152, 1326, 1350
TaaI ACNGT 2 cut(s) 694, 1165
TaiI ACGT 5 cut(s) 224, 264, 687, 822, 935
TaqI TCGA 2 cut(s) 258, 1056
TasI AATT 5 cut(s) 584, 615, 669, 849, 1252
TauI GCSGC 2 cut(s) 440, 804
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 3 cut(s) 310, 981, 1170
TseI GCWGC 4 cut(s) 789, 1036, 1271, 1292
TspDTI ATGAA 3 cut(s) 70, 988, 1217
TspGWI ACGGA 1 cut(s) 1257
TspMI CCCGGG 1 cut(s) 287
TspRI CASTG 3 cut(s) 310, 981, 1170
Tth111I GACNNNGTC 2 cut(s) 161, 844
VneI GTGCAC 1 cut(s) 832
VpaK11BI GGWCC 3 cut(s) 284, 632, 770
XagI CCTNNNNNAGG 1 cut(s) 472
XapI RAATTY 1 cut(s) 1252
XmaI CCCGGG 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 1224
XspI CTAG 2 cut(s) 327, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.