RH6DG479600

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0011720
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG479600.1

Sequence Viewer

Length: 882 bp
ATGATGATGCTTTTCCTGTTGTTCTTGTCTCTTGCGTCTCACGCCTCCTCATCAAAAGAGCAACACATCAAAAGCATTTTCATCCTGGCCGGACAGAGCAACATGGCCGGCCGCGGAGGCGTTGTAAACGACACTCTCACCGGAATCACCACCTGGGACGGCGTCGTTCCTCCAGAGTGCCTCCCCAACAAATCCATCTTCCGACTCAGCGCAAACCTCACGTGGGTTGAAGCCCGCGAGCCTCTCCACGCTGATATCGACGTGGAGAAGACGAATGGGATCGGACCCGGGATGCCATTCGCCAGTGCGATCCTTGCCAATCGTGCTAGTAGTATTGGTGCGATTGGTTTAGTCCCGTGCGCAATCGGCGGGACTAACCTAAGCCAATGGGAACGAGGGTCTTTCCTATACAGACAGATGATTGGGAGAGCCAAGGCGGCTTTGTTAAAGGTGAATGATAATGGCGCCTCAATCAGGGGACTACTCTGGTATCAAGGTGAGAGCGATACGACTAGTATAGAAGACGCCCGATCTTACAAAGCCAGATTCGAAACCTTCTTTGGAGACCTCCGGTTGGATCTGCAATTTCCTATGCTTCCAATAATCCAGGGAACTGAAGGTGTAAACGAGCAAGGTGGAGTGGAGACCAAGGTGGAGACAGTGGATTTCCGATCACCGGCAGGAGAAGACAAGGAACCCGTAAAGGAGAAGGTGGAAATACTTCATGAGATCAACGACGGAGGAGCAAATTCTGGAAATACCGGGGCTTCTGGCTTTGTGGGTAAAGCAGCTATTGCAGTTGCAGATACCTTTCAGTCAGCCAAGGATGCTGTTTCTGGAAAACCCAAGGATGCTGTTGCTGAAAATAAGACCACGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

293

Amino Acids

31.26

Weight (kDa)

5.51

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 24 - 207 8.5e-68 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 2 cut(s) 114, 237
AciI CCGC 5 cut(s) 112, 114, 235, 369, 437
AclWI GGATC 3 cut(s) 287, 304, 585
AcoI YGGCCR 3 cut(s) 87, 105, 109
AcsI RAATTY 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 636
AcvI CACGTG 1 cut(s) 222
AcyI GRCGYC 3 cut(s) 162, 465, 525
AfiI CCNNNNNNNGG 4 cut(s) 223, 474, 574, 676
AgsI TTSAA 1 cut(s) 230
AhlI ACTAGT 1 cut(s) 512
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 3 cut(s) 84, 152, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AluBI AGCT 1 cut(s) 791
AluI AGCT 1 cut(s) 791
Alw26I GTCTC 5 cut(s) 33, 42, 558, 638, 650
AlwI GGATC 3 cut(s) 287, 304, 585
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 3 cut(s) 87, 105, 109
ApeKI GCWGC 1 cut(s) 788
ApoI RAATTY 1 cut(s) 748
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 3 cut(s) 212, 362, 467
AspS9I GGNCC 1 cut(s) 284
AsuC2I CCSGG 3 cut(s) 288, 289, 763
AsuHPI GGTGA 5 cut(s) 130, 139, 463, 509, 666
AsuII TTCGAA 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 473, 506
BanI GGYRCC 1 cut(s) 464
BbrPI CACGTG 1 cut(s) 222
BbsI GAAGAC 3 cut(s) 275, 528, 693
BbvI GCAGC 1 cut(s) 800
BccI CCATC 1 cut(s) 203
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 3 cut(s) 86, 154, 608
BcnI CCSGG 3 cut(s) 288, 289, 763
BcoDI GTCTC 5 cut(s) 33, 42, 558, 638, 650
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 2 cut(s) 327, 513
BfoI RGCGCY 1 cut(s) 468
BglI GCCNNNNNGGC 2 cut(s) 117, 437
BisI GCNGC 3 cut(s) 112, 438, 789
BlsI GCNGC 3 cut(s) 113, 439, 790
Bme1390I CCNGG 6 cut(s) 86, 154, 288, 289, 608, 763
Bme18I GGWCC 1 cut(s) 284
BmeT110I CYCGRG 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 3 cut(s) 286, 466, 696
BmrFI CCNGG 6 cut(s) 86, 154, 288, 289, 608, 763
BmsI GCATC 3 cut(s) 282, 817, 841
BpiI GAAGAC 3 cut(s) 275, 528, 693
BpmI CTGGAG 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 380
Bpu14I TTCGAA 1 cut(s) 549
BpuMI CCSGG 3 cut(s) 288, 289, 763
BsaAI YACGTR 1 cut(s) 222
BsaHI GRCGYC 3 cut(s) 162, 465, 525
BsaI GGTCTC 2 cut(s) 558, 638
BsaJI CCNNGG 9 cut(s) 112, 153, 287, 432, 607, 648, 762, 822, 846
BsaWI WCCGGW 2 cut(s) 140, 570
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 474, 574, 676
Bse118I RCCGGY 2 cut(s) 107, 676
Bse1I ACTGG 1 cut(s) 303
BseBI CCWGG 3 cut(s) 86, 154, 608
BseDI CCNNGG 9 cut(s) 112, 153, 287, 432, 607, 648, 762, 822, 846
BseGI GGATG 4 cut(s) 81, 297, 832, 856
BseLI CCNNNNNNNGG 4 cut(s) 223, 474, 574, 676
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 2 cut(s) 37, 756
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 800
Bsh1236I CGCG 2 cut(s) 114, 237
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 89, 107, 111
BshNI GGYRCC 1 cut(s) 464
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 7 cut(s) 90, 108, 141, 288, 571, 677, 762
BslFI GGGAC 4 cut(s) 170, 338, 385, 492
BslI CCNNNNNNNGG 4 cut(s) 223, 474, 574, 676
BsmAI GTCTC 5 cut(s) 33, 42, 558, 638, 650
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 4 cut(s) 170, 338, 385, 492
BsnI GGCC 3 cut(s) 89, 107, 111
Bso31I GGTCTC 2 cut(s) 558, 638
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 549
Bsp143I GATC 6 cut(s) 279, 309, 530, 577, 671, 729
BspACI CCGC 5 cut(s) 112, 114, 235, 369, 437
BspANI GGCC 3 cut(s) 89, 107, 111
BspCNI CTCAG 1 cut(s) 219
BspFNI CGCG 2 cut(s) 114, 237
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 3 cut(s) 286, 466, 696
BspPI GGATC 3 cut(s) 287, 304, 585
BspT104I TTCGAA 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 2 cut(s) 558, 638
BsrFI RCCGGY 2 cut(s) 107, 676
BsrI ACTGG 1 cut(s) 303
BssAI RCCGGY 2 cut(s) 107, 676
BssECI CCNNGG 9 cut(s) 112, 153, 287, 432, 607, 648, 762, 822, 846
BssMI GATC 6 cut(s) 279, 309, 530, 577, 671, 729
BssNI GRCGYC 3 cut(s) 162, 465, 525
BssT1I CCWWGG 4 cut(s) 432, 648, 822, 846
Bst2UI CCWGG 3 cut(s) 86, 154, 608
Bst4CI ACNGT 1 cut(s) 661
BstACI GRCGYC 3 cut(s) 162, 465, 525
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 549
BstC8I GCNNGC 3 cut(s) 109, 235, 239
BstDEI CTNAG 2 cut(s) 206, 380
BstDSI CCRYGG 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 472
BstF5I GGATG 4 cut(s) 81, 297, 832, 856
BstFNI CGCG 2 cut(s) 114, 237
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 3 cut(s) 212, 362, 467
BstKTI GATC 6 cut(s) 282, 312, 533, 580, 674, 732
BstMAI GTCTC 5 cut(s) 33, 42, 558, 638, 650
BstMBI GATC 6 cut(s) 279, 309, 530, 577, 671, 729
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 8 cut(s) 41, 117, 314, 323, 366, 437, 794, 827
BstNI CCWGG 3 cut(s) 86, 154, 608
BstSCI CCNGG 6 cut(s) 84, 152, 286, 287, 606, 761
BstUI CGCG 2 cut(s) 114, 237
BstV1I GCAGC 1 cut(s) 800
BstV2I GAAGAC 3 cut(s) 275, 528, 693
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BstZI CGGCCG 1 cut(s) 109
BsuRI GGCC 3 cut(s) 89, 107, 111
BtgI CCRYGG 1 cut(s) 112
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 81, 297, 832, 856
BtsIMutI CAGTG 2 cut(s) 310, 666
Cac8I GCNNGC 3 cut(s) 109, 235, 239
CciI TCATGA 1 cut(s) 724
CfoI GCGC 3 cut(s) 212, 362, 467
Cfr10I RCCGGY 2 cut(s) 107, 676
Cfr13I GGNCC 1 cut(s) 284
Cfr42I CCGCGG 1 cut(s) 115
Cfr9I CCCGGG 1 cut(s) 287
CseI GACGC 3 cut(s) 24, 151, 533
CviAII CATG 2 cut(s) 103, 725
DdeI CTNAG 2 cut(s) 206, 380
DinI GGCGCC 1 cut(s) 466
DpnI GATC 6 cut(s) 281, 311, 532, 579, 673, 731
DpnII GATC 6 cut(s) 279, 309, 530, 577, 671, 729
EaeI YGGCCR 3 cut(s) 87, 105, 109
EagI CGGCCG 1 cut(s) 109
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 4 cut(s) 432, 648, 822, 846
Eco31I GGTCTC 2 cut(s) 558, 638
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 284
Eco52I CGGCCG 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 636
Eco72I CACGTG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 472
EcoRII CCWGG 3 cut(s) 84, 152, 606
EcoRV GATATC 1 cut(s) 256
EcoT14I CCWWGG 4 cut(s) 432, 648, 822, 846
EgeI GGCGCC 1 cut(s) 466
EheI GGCGCC 1 cut(s) 466
ErhI CCWWGG 4 cut(s) 432, 648, 822, 846
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 2 cut(s) 106, 728
FaiI YATR 5 cut(s) 104, 409, 518, 593, 726
FaqI GGGAC 4 cut(s) 170, 338, 385, 492
FatI CATG 2 cut(s) 102, 724
FauI CCCGC 2 cut(s) 242, 362
Fnu4HI GCNGC 3 cut(s) 112, 438, 789
FokI GGATG 4 cut(s) 68, 304, 839, 863
FseI GGCCGGCC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 112, 438, 789
FspBI CTAG 2 cut(s) 327, 513
FspI TGCGCA 1 cut(s) 361
GlaI GCGC 3 cut(s) 211, 361, 466
GluI GCNGC 3 cut(s) 112, 438, 789
GsuI CTGGAG 1 cut(s) 156
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 3 cut(s) 89, 107, 111
HapII CCGG 7 cut(s) 90, 108, 141, 288, 571, 677, 762
HgaI GACGC 3 cut(s) 24, 151, 533
HhaI GCGC 3 cut(s) 212, 362, 467
Hin1I GRCGYC 3 cut(s) 162, 465, 525
Hin1II CATG 2 cut(s) 106, 728
Hin6I GCGC 3 cut(s) 210, 360, 465
HinP1I GCGC 3 cut(s) 210, 360, 465
HinfI GANTC 3 cut(s) 144, 204, 546
HpaII CCGG 7 cut(s) 90, 108, 141, 288, 571, 677, 762
HphI GGTGA 5 cut(s) 130, 139, 463, 509, 666
Hpy166II GTNNAC 2 cut(s) 127, 625
Hpy188I TCNGA 3 cut(s) 203, 284, 671
Hpy188III TCNNGA 4 cut(s) 173, 725, 753, 837
Hpy8I GTNNAC 2 cut(s) 127, 625
Hpy99I CGWCG 3 cut(s) 167, 263, 740
HpyAV CCTTC 3 cut(s) 565, 611, 703
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4IV ACGT 2 cut(s) 221, 261
HpyCH4V TGCA 3 cut(s) 583, 797, 803
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 117, 314, 323, 366, 437, 794, 827
HpyF3I CTNAG 2 cut(s) 206, 380
HpySE526I ACGT 2 cut(s) 221, 261
Hsp92I GRCGYC 3 cut(s) 162, 465, 525
Hsp92II CATG 2 cut(s) 106, 728
HspAI GCGC 3 cut(s) 210, 360, 465
KasI GGCGCC 1 cut(s) 464
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 6 cut(s) 279, 309, 530, 577, 671, 729
LmnI GCTCC 1 cut(s) 743
Lsp1109I GCAGC 1 cut(s) 800
LweI GCATC 3 cut(s) 282, 817, 841
MaeI CTAG 2 cut(s) 327, 513
MaeII ACGT 2 cut(s) 221, 261
MalI GATC 6 cut(s) 281, 311, 532, 579, 673, 731
MboI GATC 6 cut(s) 279, 309, 530, 577, 671, 729
MboII GAAGA 4 cut(s) 190, 280, 533, 698
MflI RGATCY 1 cut(s) 577
MluCI AATT 2 cut(s) 584, 748
Mly113I GGCGCC 1 cut(s) 465
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 2 cut(s) 226, 555
MroNI GCCGGC 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 720
MseI TTAA 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 7 cut(s) 90, 108, 141, 288, 571, 677, 762
MspR9I CCNGG 6 cut(s) 86, 154, 288, 289, 608, 763
MvaI CCWGG 3 cut(s) 86, 154, 608
MvnI CGCG 2 cut(s) 114, 237
MwoI GCNNNNNNNGC 8 cut(s) 41, 117, 314, 323, 366, 437, 794, 827
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 465
NciI CCSGG 3 cut(s) 288, 289, 763
NdeII GATC 6 cut(s) 279, 309, 530, 577, 671, 729
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 2 cut(s) 106, 728
NlaIV GGNNCC 3 cut(s) 286, 466, 696
NsbI TGCGCA 1 cut(s) 361
NspV TTCGAA 1 cut(s) 549
PagI TCATGA 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdiI GCCGGC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 2 cut(s) 144, 546
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 3 cut(s) 113, 439, 790
PleI GAGTC 1 cut(s) 198
PluTI GGCGCC 1 cut(s) 468
PmaCI CACGTG 1 cut(s) 222
PmlI CACGTG 1 cut(s) 222
PpsI GAGTC 1 cut(s) 198
Ppu21I YACGTR 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 84, 152, 606
PspCI CACGTG 1 cut(s) 222
PspGI CCWGG 3 cut(s) 84, 152, 606
PspN4I GGNNCC 3 cut(s) 286, 466, 696
PspPI GGNCC 1 cut(s) 284
PsuI RGATCY 1 cut(s) 577
PsyI GACNNNGTC 1 cut(s) 161
RigI GGCCGGCC 1 cut(s) 111
SacII CCGCGG 1 cut(s) 115
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 3 cut(s) 112, 438, 789
Sau3AI GATC 6 cut(s) 279, 309, 530, 577, 671, 729
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 6 cut(s) 86, 154, 288, 289, 608, 763
SfaNI GCATC 3 cut(s) 282, 817, 841
SfoI GGCGCC 1 cut(s) 466
Sfr303I CCGCGG 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 549
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 1 cut(s) 284
SmaI CCCGGG 1 cut(s) 289
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 2 cut(s) 584, 748
SsiI CCGC 5 cut(s) 112, 114, 235, 369, 437
SspDI GGCGCC 1 cut(s) 464
SspMI CTAG 2 cut(s) 327, 513
StyD4I CCNGG 6 cut(s) 84, 152, 286, 287, 606, 761
StyI CCWWGG 4 cut(s) 432, 648, 822, 846
TaaI ACNGT 1 cut(s) 661
TaiI ACGT 2 cut(s) 224, 264
TaqI TCGA 2 cut(s) 258, 549
TasI AATT 2 cut(s) 584, 748
TauI GCSGC 2 cut(s) 114, 440
TfiI GAWTC 2 cut(s) 144, 546
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 2 cut(s) 310, 666
TseI GCWGC 1 cut(s) 788
TspDTI ATGAA 2 cut(s) 70, 713
TspGWI ACGGA 1 cut(s) 753
TspMI CCCGGG 1 cut(s) 287
TspRI CASTG 2 cut(s) 310, 666
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 284
XagI CCTNNNNNAGG 1 cut(s) 472
XapI RAATTY 1 cut(s) 748
XmaI CCCGGG 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 2 cut(s) 327, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.