RLG00000010622

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011720
Physical Location & Seq
Reverse (-)
3043516 .. 3044786
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010622

Sequence Viewer

Length: 891 bp
ATGATGCTTTTCCTGTTGTTCTTGTCTCTTGCGTCTCACGCCTCCTCATCAAAAGAGCAACACATCAAAAGCATTTTCATCCTGGCCGGACAGAGCAACATGGCCGGCCGCGGAGGCGTTGTAAACGACACTCTCACCGGAATCACCACCTGGGACGGCGTCGTTCCTCCAGAGTGCCTCCCCAACAAATCCATCTTCCGACTCAGCGCAAACCTCACGTGGGTTGAAGCCCGCGAGCCTCTCCACGCTGATATCGACGTGGAGAAGACGAATGGGATCGGACCCGGGATGCCATTCGCCAGTGCGATCCTTGCCAATCGTGCTAGTAGTATTGGTGCGATTGGTTTAGTCCCGTGCGCAATCGGCGGGACTAACCTAAGCCAATGGGAACGAGGGTCTTTCCTATACAGACAGATGATTGGGAGAGCCAAGGCGGCTTTGTTAAAGGTGAATGATAATGGCGCCTCAATCAGCGGATTACTCTGGTATCAAGGTGAGAGCGATACTACTAGTATAGAAGACGCCCGATCTTACAAAGCCAGATTGGAAACCTTCTTTGGAGACCTCCGGTTGGATCTGCAATTTCCTATGCTTCCAATAATCCAGGTTGCAATTGCATCTGGGTCAGGACCATACATAGAGACCATTAGAGAAGCTCAGATGGGAATTGACTTACTAAACGTGCGAACCGTTGATGCCAAGGGTCTAACATTAGAACCTGATAGGTTGCACCTCACTTCCCCGAGTCAGGTTCGACTAGGGGAAATGTTGGCTGACGCATATTTTCAGTTAATTCCCAGCACTAGAAGCAATAATGCTCGGAGAAATGGACATCCTTGTTTGATCATCCATTTTGTAATTGGCTTGTTATGGAAAATTTTGACTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

297

Amino Acids

32.2

Weight (kDa)

7.02

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 23 - 263 8.2e-89 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 461
AccII CGCG 2 cut(s) 111, 234
AciI CCGC 6 cut(s) 109, 111, 232, 366, 434, 474
AclWI GGATC 3 cut(s) 284, 301, 582
AcoI YGGCCR 3 cut(s) 84, 102, 106
AcsI RAATTY 1 cut(s) 876
AcvI CACGTG 1 cut(s) 219
AcyI GRCGYC 3 cut(s) 159, 462, 522
AfiI CCNNNNNNNGG 3 cut(s) 220, 571, 748
AgsI TTSAA 1 cut(s) 227
AhlI ACTAGT 1 cut(s) 509
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 3 cut(s) 81, 149, 603
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 1 cut(s) 656
AluI AGCT 1 cut(s) 656
Alw26I GTCTC 4 cut(s) 30, 39, 555, 635
AlwI GGATC 3 cut(s) 284, 301, 582
Ama87I CYCGRG 2 cut(s) 284, 742
AoxI GGCC 3 cut(s) 84, 102, 106
ApoI RAATTY 1 cut(s) 876
AspLEI GCGC 3 cut(s) 209, 359, 464
AspS9I GGNCC 2 cut(s) 281, 629
AsuC2I CCSGG 2 cut(s) 285, 286
AsuHPI GGTGA 4 cut(s) 127, 136, 460, 506
AvaI CYCGRG 2 cut(s) 284, 742
AvaII GGWCC 2 cut(s) 281, 629
BaeI ACNNNNGTAYC 2 cut(s) 470, 503
BanI GGYRCC 1 cut(s) 461
BbrPI CACGTG 1 cut(s) 219
BbsI GAAGAC 2 cut(s) 272, 525
BccI CCATC 2 cut(s) 200, 655
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 3 cut(s) 83, 151, 605
BclI TGATCA 1 cut(s) 843
BcnI CCSGG 2 cut(s) 285, 286
BcoDI GTCTC 4 cut(s) 30, 39, 555, 635
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 4 cut(s) 324, 510, 758, 804
BfoI RGCGCY 1 cut(s) 465
BglI GCCNNNNNGGC 2 cut(s) 114, 434
BisI GCNGC 2 cut(s) 109, 435
BlsI GCNGC 2 cut(s) 110, 436
Bme1390I CCNGG 5 cut(s) 83, 151, 285, 286, 605
Bme18I GGWCC 2 cut(s) 281, 629
BmeT110I CYCGRG 2 cut(s) 284, 742
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 281, 629
BmiI GGNNCC 2 cut(s) 283, 463
BmrFI CCNGG 5 cut(s) 83, 151, 285, 286, 605
BmsI GCATC 3 cut(s) 279, 626, 685
BpiI GAAGAC 2 cut(s) 272, 525
BpmI CTGGAG 1 cut(s) 153
Bpu10I CCTNAGC 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 285, 286
BsaAI YACGTR 1 cut(s) 219
BsaHI GRCGYC 3 cut(s) 159, 462, 522
BsaI GGTCTC 2 cut(s) 555, 635
BsaJI CCNNGG 5 cut(s) 109, 150, 284, 429, 699
BsaWI WCCGGW 2 cut(s) 137, 567
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 571, 748
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 3 cut(s) 83, 151, 605
BseDI CCNNGG 5 cut(s) 109, 150, 284, 429, 699
BseGI GGATG 4 cut(s) 78, 294, 832, 846
BseLI CCNNNNNNNGG 3 cut(s) 220, 571, 748
BseMII CTCAG 2 cut(s) 217, 671
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 34
BseX3I CGGCCG 1 cut(s) 106
BseYI CCCAGC 1 cut(s) 797
Bsh1236I CGCG 2 cut(s) 111, 234
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 3 cut(s) 86, 104, 108
BshNI GGYRCC 1 cut(s) 461
BsiEI CGRYCG 1 cut(s) 109
BsiHKCI CYCGRG 2 cut(s) 284, 742
BsiSI CCGG 5 cut(s) 87, 105, 138, 285, 568
BslFI GGGAC 3 cut(s) 167, 335, 382
BslI CCNNNNNNNGG 3 cut(s) 220, 571, 748
BsmAI GTCTC 4 cut(s) 30, 39, 555, 635
BsmBI CGTCTC 1 cut(s) 39
BsmFI GGGAC 3 cut(s) 167, 335, 382
BsnI GGCC 3 cut(s) 86, 104, 108
Bso31I GGTCTC 2 cut(s) 555, 635
BsoBI CYCGRG 2 cut(s) 284, 742
Bsp143I GATC 5 cut(s) 276, 306, 527, 574, 843
BspACI CCGC 6 cut(s) 109, 111, 232, 366, 434, 474
BspANI GGCC 3 cut(s) 86, 104, 108
BspCNI CTCAG 2 cut(s) 216, 670
BspFNI CGCG 2 cut(s) 111, 234
BspLI GGNNCC 2 cut(s) 283, 463
BspPI GGATC 3 cut(s) 284, 301, 582
BspT107I GGYRCC 1 cut(s) 461
BspTNI GGTCTC 2 cut(s) 555, 635
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 1 cut(s) 300
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 5 cut(s) 109, 150, 284, 429, 699
BssMI GATC 5 cut(s) 276, 306, 527, 574, 843
BssNI GRCGYC 3 cut(s) 159, 462, 522
BssT1I CCWWGG 2 cut(s) 429, 699
Bst2UI CCWGG 3 cut(s) 83, 151, 605
Bst4CI ACNGT 1 cut(s) 691
BstACI GRCGYC 3 cut(s) 159, 462, 522
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 3 cut(s) 106, 232, 236
BstDEI CTNAG 3 cut(s) 203, 377, 657
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 4 cut(s) 78, 294, 832, 846
BstFNI CGCG 2 cut(s) 111, 234
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 3 cut(s) 209, 359, 464
BstKTI GATC 5 cut(s) 279, 309, 530, 577, 846
BstMAI GTCTC 4 cut(s) 30, 39, 555, 635
BstMBI GATC 5 cut(s) 276, 306, 527, 574, 843
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 7 cut(s) 38, 114, 311, 320, 363, 434, 807
BstNI CCWGG 3 cut(s) 83, 151, 605
BstSCI CCNGG 5 cut(s) 81, 149, 283, 284, 603
BstUI CGCG 2 cut(s) 111, 234
BstV2I GAAGAC 2 cut(s) 272, 525
BstX2I RGATCY 1 cut(s) 574
BstYI RGATCY 1 cut(s) 574
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 3 cut(s) 86, 104, 108
BtgI CCRYGG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 4 cut(s) 78, 294, 832, 846
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 3 cut(s) 106, 232, 236
CfoI GCGC 3 cut(s) 209, 359, 464
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 281, 629
Cfr42I CCGCGG 1 cut(s) 112
Cfr9I CCCGGG 1 cut(s) 284
CseI GACGC 4 cut(s) 21, 148, 530, 785
CviAII CATG 1 cut(s) 100
DdeI CTNAG 3 cut(s) 203, 377, 657
DinI GGCGCC 1 cut(s) 463
DpnI GATC 5 cut(s) 278, 308, 529, 576, 845
DpnII GATC 5 cut(s) 276, 306, 527, 574, 843
EaeI YGGCCR 3 cut(s) 84, 102, 106
EagI CGGCCG 1 cut(s) 106
EclXI CGGCCG 1 cut(s) 106
Eco130I CCWWGG 2 cut(s) 429, 699
Eco31I GGTCTC 2 cut(s) 555, 635
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 281, 629
Eco52I CGGCCG 1 cut(s) 106
Eco72I CACGTG 1 cut(s) 219
Eco88I CYCGRG 2 cut(s) 284, 742
EcoRII CCWGG 3 cut(s) 81, 149, 603
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 429, 699
EgeI GGCGCC 1 cut(s) 463
EheI GGCGCC 1 cut(s) 463
ErhI CCWWGG 2 cut(s) 429, 699
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 1 cut(s) 103
FaiI YATR 9 cut(s) 101, 406, 515, 590, 634, 638, 781, 871, 889
FaqI GGGAC 3 cut(s) 167, 335, 382
FatI CATG 1 cut(s) 99
FauI CCCGC 2 cut(s) 239, 359
FbaI TGATCA 1 cut(s) 843
Fnu4HI GCNGC 2 cut(s) 109, 435
FokI GGATG 4 cut(s) 65, 301, 819, 833
FseI GGCCGGCC 1 cut(s) 108
Fsp4HI GCNGC 2 cut(s) 109, 435
FspBI CTAG 4 cut(s) 324, 510, 758, 804
FspI TGCGCA 1 cut(s) 358
GlaI GCGC 3 cut(s) 208, 358, 463
GluI GCNGC 2 cut(s) 109, 435
GsaI CCCAGC 1 cut(s) 801
GsuI CTGGAG 1 cut(s) 153
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 3 cut(s) 86, 104, 108
HapII CCGG 5 cut(s) 87, 105, 138, 285, 568
HgaI GACGC 4 cut(s) 21, 148, 530, 785
HhaI GCGC 3 cut(s) 209, 359, 464
Hin1I GRCGYC 3 cut(s) 159, 462, 522
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 3 cut(s) 207, 357, 462
HinP1I GCGC 3 cut(s) 207, 357, 462
HinfI GANTC 3 cut(s) 141, 201, 745
HpaII CCGG 5 cut(s) 87, 105, 138, 285, 568
HphI GGTGA 4 cut(s) 127, 136, 460, 506
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 200, 281, 660, 822
Hpy188III TCNNGA 2 cut(s) 170, 627
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 2 cut(s) 164, 260
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 1 cut(s) 691
HpyCH4IV ACGT 3 cut(s) 218, 258, 681
HpyCH4V TGCA 4 cut(s) 580, 611, 617, 730
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 114, 311, 320, 363, 434, 807
HpyF3I CTNAG 3 cut(s) 203, 377, 657
HpySE526I ACGT 3 cut(s) 218, 258, 681
Hsp92I GRCGYC 3 cut(s) 159, 462, 522
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 3 cut(s) 207, 357, 462
KasI GGCGCC 1 cut(s) 461
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 843
KspI CCGCGG 1 cut(s) 112
Kzo9I GATC 5 cut(s) 276, 306, 527, 574, 843
LweI GCATC 3 cut(s) 279, 626, 685
MaeI CTAG 4 cut(s) 324, 510, 758, 804
MaeII ACGT 3 cut(s) 218, 258, 681
MalI GATC 5 cut(s) 278, 308, 529, 576, 845
MboI GATC 5 cut(s) 276, 306, 527, 574, 843
MboII GAAGA 3 cut(s) 187, 277, 530
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 574
MluCI AATT 6 cut(s) 581, 612, 666, 792, 858, 876
Mly113I GGCGCC 1 cut(s) 462
MlyI GAGTC 2 cut(s) 195, 754
MmeI TCCRAC 2 cut(s) 223, 552
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 2 cut(s) 443, 791
MspA1I CMGCKG 2 cut(s) 111, 474
MspI CCGG 5 cut(s) 87, 105, 138, 285, 568
MspR9I CCNGG 5 cut(s) 83, 151, 285, 286, 605
MunI CAATTG 1 cut(s) 612
MvaI CCWGG 3 cut(s) 83, 151, 605
MvnI CGCG 2 cut(s) 111, 234
MwoI GCNNNNNNNGC 7 cut(s) 38, 114, 311, 320, 363, 434, 807
NaeI GCCGGC 1 cut(s) 106
NarI GGCGCC 1 cut(s) 462
NciI CCSGG 2 cut(s) 285, 286
NdeII GATC 5 cut(s) 276, 306, 527, 574, 843
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 2 cut(s) 283, 463
NsbI TGCGCA 1 cut(s) 358
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 687
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 141
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 2 cut(s) 110, 436
PleI GAGTC 2 cut(s) 195, 753
PluTI GGCGCC 1 cut(s) 465
PmaCI CACGTG 1 cut(s) 219
PmlI CACGTG 1 cut(s) 219
PpsI GAGTC 2 cut(s) 195, 753
Ppu21I YACGTR 1 cut(s) 219
Psp6I CCWGG 3 cut(s) 81, 149, 603
PspCI CACGTG 1 cut(s) 219
PspFI CCCAGC 1 cut(s) 797
PspGI CCWGG 3 cut(s) 81, 149, 603
PspN4I GGNNCC 2 cut(s) 283, 463
PspPI GGNCC 2 cut(s) 281, 629
PsuI RGATCY 1 cut(s) 574
PsyI GACNNNGTC 1 cut(s) 158
RigI GGCCGGCC 1 cut(s) 108
SacII CCGCGG 1 cut(s) 112
SaqAI TTAA 2 cut(s) 443, 791
SatI GCNGC 2 cut(s) 109, 435
Sau3AI GATC 5 cut(s) 276, 306, 527, 574, 843
Sau96I GGNCC 2 cut(s) 281, 629
SchI GAGTC 2 cut(s) 195, 754
ScrFI CCNGG 5 cut(s) 83, 151, 285, 286, 605
SfaNI GCATC 3 cut(s) 279, 626, 685
SfoI GGCGCC 1 cut(s) 463
Sfr303I CCGCGG 1 cut(s) 112
SgrBI CCGCGG 1 cut(s) 112
SinI GGWCC 2 cut(s) 281, 629
SmaI CCCGGG 1 cut(s) 286
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 6 cut(s) 581, 612, 666, 792, 858, 876
SsiI CCGC 6 cut(s) 109, 111, 232, 366, 434, 474
SspDI GGCGCC 1 cut(s) 461
SspMI CTAG 4 cut(s) 324, 510, 758, 804
StyD4I CCNGG 5 cut(s) 81, 149, 283, 284, 603
StyI CCWWGG 2 cut(s) 429, 699
TaaI ACNGT 1 cut(s) 691
TaiI ACGT 3 cut(s) 221, 261, 684
TaqI TCGA 2 cut(s) 255, 754
TasI AATT 6 cut(s) 581, 612, 666, 792, 858, 876
TauI GCSGC 2 cut(s) 111, 437
TfiI GAWTC 1 cut(s) 141
Tru1I TTAA 2 cut(s) 443, 791
Tru9I TTAA 2 cut(s) 443, 791
TscAI CASTG 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 67
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 281, 629
XapI RAATTY 1 cut(s) 876
XcmI CCANNNNNNNNNTGG 1 cut(s) 857
XmaI CCCGGG 1 cut(s) 284
XspI CTAG 4 cut(s) 324, 510, 758, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.