FvH4_2g33800

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011720
Physical Location & Seq
Reverse (-)
25240432 .. 25242335
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33800.t1

Sequence Viewer

Length: 894 bp
ATGATGATGCTTTTCGTGTTGTTGTTGTCTCTTGCGTCTCACGCCTCCTCATCAAAAGAGCAACACATCAACAGCATTTTCATCCTGGCCGGACAGAGCAACATGGCCGGCCGCGGAGGCGTTGTCAACAACACCATCACCGGAATCACCACCTGGGACGGCGTCGTTCCTCCCGAGTGCCGCCCCAACCCATCTATCTTCCGACTCAGCGCCAACCTCACGTGGGTGGAAGCACGCGAGCCTCTCCACGCCGACATCGACGTTGAGAAGACAAATGGTATCGGACCCGGAATGCCATTCGCCAATGCGCTCTTTGCCAACCGCGCCGTTAATACTGACGCGATTGGTTTAGTTCCGTGTGCGATCGGCGGCACTAACCTAAGCGAATGGGAACGAGGGTCATTCTTATACAGGCAGATGATTGGGAGAGCCAGGGCGACTTCGCTAAAGGTGAATAGCAATGGCGCCTCAATCAGGGGACTGCTCTGGTATCAAGGTGAGAGCGATACGACTACTAGGGAAGACGCCGAATCTTACAAAGCCAGACTGGAAACCTTTTTTGGAGACCTCCGGTTGGATCTGCAATATCCTCTGCTTCCAATAATCCAGGTGGCAATTGCATCTGGGTCAGGACCATACATAGAGACAATCAGAGAAGCTCAGCTGGGAGTTGAACTACTAAATGTCCGAACAGTTGACGCCAAGGGATTAACATTAGAACCTGATAACTTGCACCTCACTACACCTAGTCAGGTTCGACTAGGAGAAATGTTGGCTGACGCATATTCTCAGTTGATTCCCAGCTCTATAAGCAATCATGCTCGAAGTAAAGGATGTCCTTGTTTGATCACCCATTTTGTTATGGGATTGCTATGGAAAATTTTGACTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

298

Amino Acids

32.31

Weight (kDa)

6.08

Isoelectric Point (pI)

28.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 25 - 263 7.8e-91 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 4 cut(s) 114, 237, 324, 341
AciI CCGC 5 cut(s) 112, 114, 181, 322, 369
AclWI GGATC 1 cut(s) 585
AcoI YGGCCR 3 cut(s) 87, 105, 109
AcsI RAATTY 1 cut(s) 879
AcvI CACGTG 1 cut(s) 222
AcyI GRCGYC 4 cut(s) 162, 465, 525, 699
AfiI CCNNNNNNNGG 3 cut(s) 223, 474, 574
AgsI TTSAA 1 cut(s) 674
AjnI CCWGG 4 cut(s) 84, 152, 431, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 3 cut(s) 659, 664, 804
AluI AGCT 3 cut(s) 659, 664, 804
Alw26I GTCTC 4 cut(s) 33, 42, 558, 638
AlwI GGATC 1 cut(s) 585
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 3 cut(s) 87, 105, 109
ApoI RAATTY 1 cut(s) 879
AspLEI GCGC 4 cut(s) 212, 310, 326, 467
AspS9I GGNCC 2 cut(s) 284, 632
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 5 cut(s) 130, 139, 463, 509, 841
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 2 cut(s) 284, 632
BanI GGYRCC 1 cut(s) 464
BbrPI CACGTG 1 cut(s) 222
BbsI GAAGAC 2 cut(s) 275, 528
BccI CCATC 2 cut(s) 143, 199
BceAI ACGGC 2 cut(s) 175, 311
BciT130I CCWGG 4 cut(s) 86, 154, 433, 608
BclI TGATCA 1 cut(s) 846
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 4 cut(s) 33, 42, 558, 638
BfaI CTAG 3 cut(s) 516, 747, 761
BfoI RGCGCY 2 cut(s) 213, 468
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 3 cut(s) 112, 181, 370
BlpI GCTNAGC 1 cut(s) 660
BlsI GCNGC 3 cut(s) 113, 182, 371
Bme1390I CCNGG 5 cut(s) 86, 154, 288, 433, 608
Bme18I GGWCC 2 cut(s) 284, 632
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 284, 632
BmiI GGNNCC 2 cut(s) 286, 466
BmrFI CCNGG 5 cut(s) 86, 154, 288, 433, 608
BmsI GCATC 1 cut(s) 629
BpiI GAAGAC 2 cut(s) 275, 528
Bpu10I CCTNAGC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 660
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 222
BsaHI GRCGYC 4 cut(s) 162, 465, 525, 699
BsaI GGTCTC 1 cut(s) 558
BsaJI CCNNGG 4 cut(s) 112, 153, 432, 702
BsaWI WCCGGW 2 cut(s) 140, 570
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 474, 574
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 552
Bse3DI GCAATG 1 cut(s) 466
BseBI CCWGG 4 cut(s) 86, 154, 433, 608
BseDI CCNNGG 4 cut(s) 112, 153, 432, 702
BseGI GGATG 2 cut(s) 81, 839
BseLI CCNNNNNNNGG 3 cut(s) 223, 474, 574
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 220, 674, 803
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 1 cut(s) 37
BseX3I CGGCCG 1 cut(s) 109
BseYI CCCAGC 2 cut(s) 664, 800
Bsh1236I CGCG 4 cut(s) 114, 237, 324, 341
Bsh1285I CGRYCG 2 cut(s) 112, 366
BshFI GGCC 3 cut(s) 89, 107, 111
BshNI GGYRCC 1 cut(s) 464
BsiEI CGRYCG 2 cut(s) 112, 366
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 5 cut(s) 90, 108, 141, 288, 571
BslFI GGGAC 2 cut(s) 170, 492
BslI CCNNNNNNNGG 3 cut(s) 223, 474, 574
BsmAI GTCTC 4 cut(s) 33, 42, 558, 638
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 2 cut(s) 170, 492
BsmI GAATGC 1 cut(s) 297
BsnI GGCC 3 cut(s) 89, 107, 111
Bso31I GGTCTC 1 cut(s) 558
BsoBI CYCGRG 1 cut(s) 173
Bsp143I GATC 3 cut(s) 363, 577, 846
Bsp1720I GCTNAGC 1 cut(s) 660
BspACI CCGC 5 cut(s) 112, 114, 181, 322, 369
BspANI GGCC 3 cut(s) 89, 107, 111
BspCNI CTCAG 3 cut(s) 219, 673, 802
BspFNI CGCG 4 cut(s) 114, 237, 324, 341
BspLI GGNNCC 2 cut(s) 286, 466
BspPI GGATC 1 cut(s) 585
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 558
BsrDI GCAATG 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 1 cut(s) 552
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 4 cut(s) 112, 153, 432, 702
BssMI GATC 3 cut(s) 363, 577, 846
BssNI GRCGYC 4 cut(s) 162, 465, 525, 699
BssT1I CCWWGG 1 cut(s) 702
Bst2UI CCWGG 4 cut(s) 86, 154, 433, 608
Bst4CI ACNGT 1 cut(s) 694
BstACI GRCGYC 4 cut(s) 162, 465, 525, 699
BstBAI YACGTR 1 cut(s) 222
BstC8I GCNNGC 3 cut(s) 109, 235, 239
BstDEI CTNAG 4 cut(s) 206, 380, 660, 789
BstDSI CCRYGG 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 472
BstF5I GGATG 2 cut(s) 81, 839
BstFNI CGCG 4 cut(s) 114, 237, 324, 341
BstH2I RGCGCY 2 cut(s) 213, 468
BstHHI GCGC 4 cut(s) 212, 310, 326, 467
BstKTI GATC 3 cut(s) 366, 580, 849
BstMAI GTCTC 4 cut(s) 33, 42, 558, 638
BstMBI GATC 3 cut(s) 363, 577, 846
BstMCI CGRYCG 2 cut(s) 112, 366
BstMWI GCNNNNNNNGC 5 cut(s) 41, 117, 314, 323, 810
BstNI CCWGG 4 cut(s) 86, 154, 433, 608
BstSCI CCNGG 5 cut(s) 84, 152, 286, 431, 606
BstUI CGCG 4 cut(s) 114, 237, 324, 341
BstV2I GAAGAC 2 cut(s) 275, 528
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BstZI CGGCCG 1 cut(s) 109
BsuRI GGCC 3 cut(s) 89, 107, 111
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 2 cut(s) 81, 839
Cac8I GCNNGC 3 cut(s) 109, 235, 239
CfoI GCGC 4 cut(s) 212, 310, 326, 467
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 284, 632
Cfr42I CCGCGG 1 cut(s) 115
CseI GACGC 6 cut(s) 24, 151, 347, 533, 707, 788
CviAII CATG 2 cut(s) 103, 818
DdeI CTNAG 4 cut(s) 206, 380, 660, 789
DinI GGCGCC 1 cut(s) 466
DpnI GATC 3 cut(s) 365, 579, 848
DpnII GATC 3 cut(s) 363, 577, 846
EaeI YGGCCR 3 cut(s) 87, 105, 109
EagI CGGCCG 1 cut(s) 109
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 702
Eco31I GGTCTC 1 cut(s) 558
Eco47I GGWCC 2 cut(s) 284, 632
Eco52I CGGCCG 1 cut(s) 109
Eco72I CACGTG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 173
EcoNI CCTNNNNNAGG 1 cut(s) 472
EcoRII CCWGG 4 cut(s) 84, 152, 431, 606
EcoT14I CCWWGG 1 cut(s) 702
EgeI GGCGCC 1 cut(s) 466
EheI GGCGCC 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 702
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 2 cut(s) 106, 821
FaqI GGGAC 2 cut(s) 170, 492
FatI CATG 2 cut(s) 102, 817
FbaI TGATCA 1 cut(s) 846
Fnu4HI GCNGC 3 cut(s) 112, 181, 370
FokI GGATG 2 cut(s) 68, 846
FseI GGCCGGCC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 112, 181, 370
FspBI CTAG 3 cut(s) 516, 747, 761
GlaI GCGC 4 cut(s) 211, 309, 325, 466
GluI GCNGC 3 cut(s) 112, 181, 370
GsaI CCCAGC 2 cut(s) 668, 804
HaeII RGCGCY 2 cut(s) 213, 468
HaeIII GGCC 3 cut(s) 89, 107, 111
HapII CCGG 5 cut(s) 90, 108, 141, 288, 571
HgaI GACGC 6 cut(s) 24, 151, 347, 533, 707, 788
HhaI GCGC 4 cut(s) 212, 310, 326, 467
Hin1I GRCGYC 4 cut(s) 162, 465, 525, 699
Hin1II CATG 2 cut(s) 106, 821
Hin6I GCGC 4 cut(s) 210, 308, 324, 465
HinP1I GCGC 4 cut(s) 210, 308, 324, 465
HincII GTYRAC 2 cut(s) 127, 697
HindII GTYRAC 2 cut(s) 127, 697
HinfI GANTC 4 cut(s) 144, 204, 530, 796
HpaII CCGG 5 cut(s) 90, 108, 141, 288, 571
HphI GGTGA 5 cut(s) 130, 139, 463, 509, 841
Hpy166II GTNNAC 2 cut(s) 127, 697
Hpy188I TCNGA 4 cut(s) 203, 284, 653, 689
Hpy188III TCNNGA 2 cut(s) 173, 630
Hpy8I GTNNAC 2 cut(s) 127, 697
Hpy99I CGWCG 2 cut(s) 167, 263
HpyCH4III ACNGT 1 cut(s) 694
HpyCH4IV ACGT 2 cut(s) 221, 261
HpyCH4V TGCA 3 cut(s) 583, 620, 733
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 117, 314, 323, 810
HpyF3I CTNAG 4 cut(s) 206, 380, 660, 789
HpySE526I ACGT 2 cut(s) 221, 261
Hsp92I GRCGYC 4 cut(s) 162, 465, 525, 699
Hsp92II CATG 2 cut(s) 106, 821
HspAI GCGC 4 cut(s) 210, 308, 324, 465
KasI GGCGCC 1 cut(s) 464
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 846
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 3 cut(s) 363, 577, 846
LweI GCATC 1 cut(s) 629
MaeI CTAG 3 cut(s) 516, 747, 761
MaeII ACGT 2 cut(s) 221, 261
MalI GATC 3 cut(s) 365, 579, 848
MboI GATC 3 cut(s) 363, 577, 846
MboII GAAGA 3 cut(s) 190, 280, 533
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 577
MluCI AATT 2 cut(s) 615, 879
Mly113I GGCGCC 1 cut(s) 465
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 2 cut(s) 226, 555
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 2 cut(s) 330, 710
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 2 cut(s) 114, 664
MspI CCGG 5 cut(s) 90, 108, 141, 288, 571
MspR9I CCNGG 5 cut(s) 86, 154, 288, 433, 608
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 297
MvaI CCWGG 4 cut(s) 86, 154, 433, 608
MvnI CGCG 4 cut(s) 114, 237, 324, 341
MwoI GCNNNNNNNGC 5 cut(s) 41, 117, 314, 323, 810
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 465
NciI CCSGG 1 cut(s) 288
NdeII GATC 3 cut(s) 363, 577, 846
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 2 cut(s) 106, 821
NlaIV GGNNCC 2 cut(s) 286, 466
OliI CACNNNNGTG 1 cut(s) 224
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 255
PctI GAATGC 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 3 cut(s) 144, 530, 796
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 3 cut(s) 113, 182, 371
Ple19I CGATCG 1 cut(s) 366
PleI GAGTC 1 cut(s) 198
PluTI GGCGCC 1 cut(s) 468
PmaCI CACGTG 1 cut(s) 222
PmlI CACGTG 1 cut(s) 222
PpsI GAGTC 1 cut(s) 198
Ppu21I YACGTR 1 cut(s) 222
Psp6I CCWGG 4 cut(s) 84, 152, 431, 606
PspCI CACGTG 1 cut(s) 222
PspFI CCCAGC 2 cut(s) 664, 800
PspGI CCWGG 4 cut(s) 84, 152, 431, 606
PspN4I GGNNCC 2 cut(s) 286, 466
PspPI GGNCC 2 cut(s) 284, 632
PsuI RGATCY 1 cut(s) 577
PsyI GACNNNGTC 1 cut(s) 161
PvuI CGATCG 1 cut(s) 366
PvuII CAGCTG 1 cut(s) 664
RigI GGCCGGCC 1 cut(s) 111
RseI CAYNNNNRTG 1 cut(s) 224
SacII CCGCGG 1 cut(s) 115
SaqAI TTAA 2 cut(s) 330, 710
SatI GCNGC 3 cut(s) 112, 181, 370
Sau3AI GATC 3 cut(s) 363, 577, 846
Sau96I GGNCC 2 cut(s) 284, 632
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 5 cut(s) 86, 154, 288, 433, 608
SfaNI GCATC 1 cut(s) 629
SfoI GGCGCC 1 cut(s) 466
Sfr303I CCGCGG 1 cut(s) 115
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 2 cut(s) 284, 632
SmiMI CAYNNNNRTG 1 cut(s) 224
Sse9I AATT 2 cut(s) 615, 879
SsiI CCGC 5 cut(s) 112, 114, 181, 322, 369
SspDI GGCGCC 1 cut(s) 464
SspMI CTAG 3 cut(s) 516, 747, 761
StyD4I CCNGG 5 cut(s) 84, 152, 286, 431, 606
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 1 cut(s) 694
TaiI ACGT 2 cut(s) 224, 264
TaqI TCGA 3 cut(s) 258, 757, 823
TasI AATT 2 cut(s) 615, 879
TauI GCSGC 3 cut(s) 114, 183, 372
TfiI GAWTC 3 cut(s) 144, 530, 796
Tru1I TTAA 2 cut(s) 330, 710
Tru9I TTAA 2 cut(s) 330, 710
TspDTI ATGAA 1 cut(s) 70
TspGWI ACGGA 1 cut(s) 345
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 284, 632
XagI CCTNNNNNAGG 1 cut(s) 472
XapI RAATTY 1 cut(s) 879
XspI CTAG 3 cut(s) 516, 747, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.