AT3G53140
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011057
Physical Location & Seq
Forward (+)
19695643 .. 19697542
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53140.1

Sequence Viewer

Length: 1080 bp
ATGGAGAACGAAAGCTCAGAGAGTAGAAACAGAGCTCGTCTTGCCATTATGGAGCTTGCTAACATGATTAGCGTTCCCATGTCTCTCAATGCCGCCGTGCGACTAGGCATTGCCGACGCCATTTGGAACGGCGGAGCCAATTCTCCTCTCTCTGCCGCCGAGATCCTCCCTCGCCTCCACCTACCATCTCACACTACCATTGGTGGCGACCCCGAGAATCTTCAGCGTATACTTCGGATGCTCACCAGCTACGGTGTCTTCTCCGAACACCTTGTTGGATCCATTGAGAGGAAATACTCTCTTACGGACGTCGGAAAAACTCTTGTAACCGACTCCGGCGGCCTCTCTTACGCTGCCTACGTCCTCCAACATCACCAGGAGGCGTTGATGCGAGCATGGCCACTAGTTCACACGGCAGTGGTGGAGCCGGAGACAGAGCCGTACGTGAAAGCAAACGGCGAGGCGGCATACGCTCAGTATGGGAAAAGTGAGGAGATGAATGGTCTAATGCAAAAGGCAATGTCTGGCGTATCTGTACCGTTCATGAAAGCTATATTAGACGGCTACGATGGGTTTAAATCAGTGGATATTTTGGTTGACGTAGGAGGTAGTGCAGGGGATTGTCTGCGTATGATCCTTCAACAATTTCCTAACGTCCGTGAAGGGATTAATTTCGATTTACCTGAAGTTGTTGCCAAAGCCCCCAATATTCCTGGAGTGACTCACGTGGGTGGGGATATGTTCCAATCAGTTCCTAGCGCTGACGCTATCTTCATGAAGTGGGTGTTAACGACATGGACGGATGAAGAATGCAAGCAGATAATGAAGAATTGCTACAACGCGTTACCAGTTGGAGGAAAGCTGATTGCGTGTGAGCCGGTCTTGCCTAAGGAAACCGATGAAAGTCACCGGACTCGTGCCTTGTTAGAAGGTGACATCTTTGTCATGACCATCTATAGAACCAAAGGTAAGCATAGAACCGAAGAAGAGTTTATAGAGCTTGGTCTCTCCGCGGGATTCCCTACTTTTCGACCTTTCTACATTGATTACTTCTACACCATCTTAGAGTTTCAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

359

Amino Acids

39.69

Weight (kDa)

5.28

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 17 - 111 2.5e-18 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 138 - 340 1.4e-46 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53140
fragaria_vesca FvH4_6g17130 FvH4_6g17130 FvH4_6g17130
malus_domestica MD12G1103500.v1.1 MD14G1097900.v1.1
prunus_persica Prupe.7G123600_v2.0.a1
pyrus_communis pycom12g10200
rosa_chinensis RchiOBHm_Chr3g0471211
rosa_laevigata RLG00000024197
rosa_multiflora Rmu_co8165034.1_g000001 Rmu_sc0010073.1_g000018 Rmu_sc0031310.1_g000002
rosa_roxburghii Rroxscaffold_6G00410220
rosa_rugosa Rorug03G0116200
rosa_samantha Rh3AG165100 Rh3BG190700 Rh3CG181000 Rh3DG185100
rosa_wichuraiana Rw3G015570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 941
AatII GACGTC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 229
AccII CGCG 2 cut(s) 842, 1013
AciI CCGC 7 cut(s) 93, 132, 156, 339, 464, 1011, 1013
AclWI GGATC 4 cut(s) 157, 273, 286, 628
AcoI YGGCCR 1 cut(s) 398
AcuI CTGAAG 2 cut(s) 206, 705
AcvI CACGTG 1 cut(s) 727
AcyI GRCGYC 2 cut(s) 117, 309
AfaI GTAC 2 cut(s) 443, 537
AfeI AGCGCT 1 cut(s) 760
AfiI CCNNNNNNNGG 2 cut(s) 288, 854
AflIII ACRYGT 1 cut(s) 840
AgsI TTSAA 1 cut(s) 641
AhlI ACTAGT 1 cut(s) 403
AjnI CCWGG 2 cut(s) 375, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AleI CACNNNNGTG 2 cut(s) 416, 729
AluBI AGCT 7 cut(s) 15, 35, 55, 249, 551, 862, 1000
AluI AGCT 7 cut(s) 15, 35, 55, 249, 551, 862, 1000
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 3 cut(s) 87, 425, 1010
AlwI GGATC 4 cut(s) 157, 273, 286, 628
Ama87I CYCGRG 1 cut(s) 212
Aor51HI AGCGCT 1 cut(s) 760
AoxI GGCC 2 cut(s) 340, 398
ApeKI GCWGC 1 cut(s) 353
AseI ATTAAT 1 cut(s) 669
AspLEI GCGC 1 cut(s) 761
AsuHPI GGTGA 4 cut(s) 235, 365, 899, 944
AvaI CYCGRG 1 cut(s) 212
AxyI CCTNAGG 1 cut(s) 888
BalI TGGCCA 1 cut(s) 400
BamHI GGATCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 818, 850
BauI CACGAG 1 cut(s) 915
BbrPI CACGTG 1 cut(s) 727
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 340
BccI CCATC 4 cut(s) 193, 563, 959, 1067
BceAI ACGGC 6 cut(s) 80, 145, 424, 429, 472, 577
BciT130I CCWGG 2 cut(s) 377, 714
BcoDI GTCTC 3 cut(s) 87, 425, 1010
BcuI ACTAGT 1 cut(s) 403
BfaI CTAG 3 cut(s) 104, 404, 756
BfmI CTRYAG 1 cut(s) 955
BfoI RGCGCY 1 cut(s) 762
BisI GCNGC 5 cut(s) 93, 156, 340, 354, 465
BlsI GCNGC 5 cut(s) 94, 157, 341, 355, 466
Bme1390I CCNGG 2 cut(s) 377, 714
BmeT110I CYCGRG 1 cut(s) 212
BmiI GGNNCC 3 cut(s) 136, 280, 426
BmrFI CCNGG 2 cut(s) 377, 714
BmsI GCATC 2 cut(s) 228, 378
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 735
BsaAI YACGTR 2 cut(s) 445, 727
BsaHI GRCGYC 2 cut(s) 117, 309
BsaI GGTCTC 1 cut(s) 1010
BsaJI CCNNGG 1 cut(s) 1011
BsaWI WCCGGW 1 cut(s) 909
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 854
Bse118I RCCGGY 1 cut(s) 877
Bse1I ACTGG 1 cut(s) 848
Bse21I CCTNAGG 1 cut(s) 888
Bse3DI GCAATG 2 cut(s) 108, 525
BseBI CCWGG 2 cut(s) 377, 714
BseDI CCNNGG 1 cut(s) 1011
BseGI GGATG 2 cut(s) 243, 808
BseLI CCNNNNNNNGG 2 cut(s) 288, 854
BseMI GCAATG 2 cut(s) 108, 525
BseMII CTCAG 2 cut(s) 30, 488
BseNI ACTGG 1 cut(s) 848
BseRI GAGGAG 2 cut(s) 135, 506
BseXI GCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 633
Bsh1236I CGCG 2 cut(s) 842, 1013
BshFI GGCC 2 cut(s) 342, 400
BsiHKAI GWGCWC 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 4 cut(s) 336, 428, 878, 910
BsiWI CGTACG 1 cut(s) 441
BslI CCNNNNNNNGG 2 cut(s) 288, 854
BsmAI GTCTC 3 cut(s) 87, 425, 1010
BsmI GAATGC 1 cut(s) 815
BsnI GGCC 2 cut(s) 342, 400
Bso31I GGTCTC 1 cut(s) 1010
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 3 cut(s) 162, 278, 633
BspACI CCGC 7 cut(s) 93, 132, 156, 339, 464, 1011, 1013
BspANI GGCC 2 cut(s) 342, 400
BspCNI CTCAG 2 cut(s) 29, 487
BspFNI CGCG 2 cut(s) 842, 1013
BspHI TCATGA 3 cut(s) 543, 774, 945
BspLI GGNNCC 3 cut(s) 136, 280, 426
BspPI GGATC 4 cut(s) 157, 273, 286, 628
BspTNI GGTCTC 1 cut(s) 1010
BsrDI GCAATG 2 cut(s) 108, 525
BsrFI RCCGGY 1 cut(s) 877
BsrI ACTGG 1 cut(s) 848
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 1 cut(s) 1011
BssMI GATC 3 cut(s) 162, 278, 633
BssNAI GTATAC 1 cut(s) 230
BssNI GRCGYC 2 cut(s) 117, 309
BssSI CACGAG 1 cut(s) 915
Bst1107I GTATAC 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 915
Bst2UI CCWGG 2 cut(s) 377, 714
Bst4CI ACNGT 2 cut(s) 254, 540
Bst6I CTCTTC 1 cut(s) 981
BstACI GRCGYC 2 cut(s) 117, 309
BstBAI YACGTR 2 cut(s) 445, 727
BstC8I GCNNGC 3 cut(s) 57, 393, 815
BstDEI CTNAG 4 cut(s) 16, 474, 888, 1063
BstDSI CCRYGG 1 cut(s) 1011
BstF5I GGATG 2 cut(s) 243, 808
BstFNI CGCG 2 cut(s) 842, 1013
BstH2I RGCGCY 1 cut(s) 762
BstHHI GCGC 1 cut(s) 761
BstKTI GATC 3 cut(s) 165, 281, 636
BstMAI GTCTC 3 cut(s) 87, 425, 1010
BstMBI GATC 3 cut(s) 162, 278, 633
BstMWI GCNNNNNNNGC 4 cut(s) 41, 397, 470, 883
BstNI CCWGG 2 cut(s) 377, 714
BstSCI CCNGG 2 cut(s) 375, 712
BstSFI CTRYAG 1 cut(s) 955
BstUI CGCG 2 cut(s) 842, 1013
BstV1I GCAGC 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 2 cut(s) 162, 278
BstYI RGATCY 2 cut(s) 162, 278
BstZ17I GTATAC 1 cut(s) 230
Bsu36I CCTNAGG 1 cut(s) 888
BsuRI GGCC 2 cut(s) 342, 400
BtgI CCRYGG 1 cut(s) 1011
BtsCI GGATG 2 cut(s) 243, 808
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 2 cut(s) 423, 588
Cac8I GCNNGC 3 cut(s) 57, 393, 815
CciI TCATGA 3 cut(s) 543, 774, 945
CfoI GCGC 1 cut(s) 761
Cfr10I RCCGGY 1 cut(s) 877
Cfr42I CCGCGG 1 cut(s) 1014
CseI GACGC 2 cut(s) 125, 773
Csp6I GTAC 2 cut(s) 442, 536
CviAII CATG 7 cut(s) 64, 79, 396, 544, 775, 795, 946
CviQI GTAC 2 cut(s) 442, 536
DdeI CTNAG 4 cut(s) 16, 474, 888, 1063
DpnI GATC 3 cut(s) 164, 280, 635
DpnII GATC 3 cut(s) 162, 278, 633
DraI TTTAAA 1 cut(s) 577
DrdI GACNNNNNNGTC 1 cut(s) 941
DseDI GACNNNNNNGTC 1 cut(s) 941
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 981
EarI CTCTTC 1 cut(s) 981
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 1010
Eco47III AGCGCT 1 cut(s) 760
Eco53kI GAGCTC 1 cut(s) 35
Eco57I CTGAAG 2 cut(s) 206, 705
Eco72I CACGTG 1 cut(s) 727
Eco81I CCTNAGG 1 cut(s) 888
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 35
EcoRII CCWGG 2 cut(s) 375, 712
EcoT38I GRGCYC 1 cut(s) 37
FaeI CATG 7 cut(s) 67, 82, 399, 547, 778, 798, 949
FatI CATG 7 cut(s) 63, 78, 395, 543, 774, 794, 945
FauI CCCGC 1 cut(s) 1006
FblI GTMKAC 1 cut(s) 229
Fnu4HI GCNGC 5 cut(s) 93, 156, 340, 354, 465
FokI GGATG 2 cut(s) 250, 815
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 5 cut(s) 93, 156, 340, 354, 465
FspBI CTAG 3 cut(s) 104, 404, 756
GlaI GCGC 1 cut(s) 760
GluI GCNGC 5 cut(s) 93, 156, 340, 354, 465
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 1 cut(s) 762
HaeIII GGCC 2 cut(s) 342, 400
HapII CCGG 4 cut(s) 336, 428, 878, 910
HgaI GACGC 2 cut(s) 125, 773
HhaI GCGC 1 cut(s) 761
Hin1I GRCGYC 2 cut(s) 117, 309
Hin1II CATG 7 cut(s) 67, 82, 399, 547, 778, 798, 949
Hin6I GCGC 1 cut(s) 759
HinP1I GCGC 1 cut(s) 759
HincII GTYRAC 2 cut(s) 598, 789
HindII GTYRAC 2 cut(s) 598, 789
HinfI GANTC 5 cut(s) 217, 332, 721, 913, 1017
HpaI GTTAAC 1 cut(s) 789
HpaII CCGG 4 cut(s) 336, 428, 878, 910
HphI GGTGA 4 cut(s) 235, 365, 899, 944
Hpy166II GTNNAC 4 cut(s) 230, 409, 598, 789
Hpy188I TCNGA 5 cut(s) 19, 237, 265, 314, 1074
Hpy188III TCNNGA 3 cut(s) 544, 775, 946
Hpy8I GTNNAC 4 cut(s) 230, 409, 598, 789
Hpy99I CGWCG 2 cut(s) 119, 314
HpyAV CCTTC 3 cut(s) 647, 656, 923
HpyCH4III ACNGT 2 cut(s) 254, 540
HpyCH4IV ACGT 6 cut(s) 309, 360, 444, 600, 654, 726
HpyCH4V TGCA 3 cut(s) 511, 614, 813
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 397, 470, 883
HpyF3I CTNAG 4 cut(s) 16, 474, 888, 1063
HpySE526I ACGT 6 cut(s) 309, 360, 444, 600, 654, 726
Hsp92I GRCGYC 2 cut(s) 117, 309
Hsp92II CATG 7 cut(s) 67, 82, 399, 547, 778, 798, 949
HspAI GCGC 1 cut(s) 759
KspAI GTTAAC 1 cut(s) 789
KspI CCGCGG 1 cut(s) 1014
Kzo9I GATC 3 cut(s) 162, 278, 633
LmnI GCTCC 3 cut(s) 52, 134, 424
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 2 cut(s) 228, 378
MaeI CTAG 3 cut(s) 104, 404, 756
MaeII ACGT 6 cut(s) 309, 360, 444, 600, 654, 726
MaeIII GTNAC 5 cut(s) 325, 718, 843, 905, 932
MalI GATC 3 cut(s) 164, 280, 635
MboI GATC 3 cut(s) 162, 278, 633
MboII GAAGA 7 cut(s) 212, 250, 763, 818, 838, 995, 998
MflI RGATCY 2 cut(s) 162, 278
MhlI GDGCHC 1 cut(s) 37
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 4 cut(s) 139, 644, 670, 829
MluI ACGCGT 1 cut(s) 840
MluNI TGGCCA 1 cut(s) 400
MlyI GAGTC 3 cut(s) 326, 715, 907
MmeI TCCRAC 4 cut(s) 256, 292, 391, 832
Mox20I TGGCCA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 400
MseI TTAA 3 cut(s) 576, 669, 788
MslI CAYNNNNRTG 2 cut(s) 416, 729
Msp20I TGGCCA 1 cut(s) 400
MspA1I CMGCKG 1 cut(s) 1013
MspI CCGG 4 cut(s) 336, 428, 878, 910
MspR9I CCNGG 2 cut(s) 377, 714
Mva1269I GAATGC 1 cut(s) 815
MvaI CCWGG 2 cut(s) 377, 714
MvnI CGCG 2 cut(s) 842, 1013
MwoI GCNNNNNNNGC 4 cut(s) 41, 397, 470, 883
NdeII GATC 3 cut(s) 162, 278, 633
NlaIII CATG 7 cut(s) 67, 82, 399, 547, 778, 798, 949
NlaIV GGNNCC 3 cut(s) 136, 280, 426
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 3 cut(s) 718, 905, 932
OliI CACNNNNGTG 2 cut(s) 416, 729
PagI TCATGA 3 cut(s) 543, 774, 945
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PctI GAATGC 1 cut(s) 815
PfeI GAWTC 2 cut(s) 217, 1017
Pfl23II CGTACG 1 cut(s) 441
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 5 cut(s) 94, 157, 341, 355, 466
PleI GAGTC 3 cut(s) 326, 715, 907
PmaCI CACGTG 1 cut(s) 727
PmlI CACGTG 1 cut(s) 727
PpsI GAGTC 3 cut(s) 326, 715, 907
Ppu21I YACGTR 2 cut(s) 445, 727
PshBI ATTAAT 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 37
Psp6I CCWGG 2 cut(s) 375, 712
PspCI CACGTG 1 cut(s) 727
PspGI CCWGG 2 cut(s) 375, 712
PspLI CGTACG 1 cut(s) 441
PspN4I GGNNCC 3 cut(s) 136, 280, 426
PsuI RGATCY 2 cut(s) 162, 278
RsaI GTAC 2 cut(s) 443, 537
RsaNI GTAC 2 cut(s) 442, 536
RseI CAYNNNNRTG 2 cut(s) 416, 729
SacI GAGCTC 1 cut(s) 37
SacII CCGCGG 1 cut(s) 1014
SaqAI TTAA 3 cut(s) 576, 669, 788
SatI GCNGC 5 cut(s) 93, 156, 340, 354, 465
Sau3AI GATC 3 cut(s) 162, 278, 633
SchI GAGTC 3 cut(s) 326, 715, 907
ScrFI CCNGG 2 cut(s) 377, 714
SduI GDGCHC 1 cut(s) 37
SfaNI GCATC 2 cut(s) 228, 378
SfcI CTRYAG 1 cut(s) 955
Sfr303I CCGCGG 1 cut(s) 1014
SgrBI CCGCGG 1 cut(s) 1014
SmiMI CAYNNNNRTG 2 cut(s) 416, 729
SpeI ACTAGT 1 cut(s) 403
Sse9I AATT 4 cut(s) 139, 644, 670, 829
SsiI CCGC 7 cut(s) 93, 132, 156, 339, 464, 1011, 1013
SspI AATATT 1 cut(s) 709
SspMI CTAG 3 cut(s) 104, 404, 756
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 2 cut(s) 375, 712
TaaI ACNGT 2 cut(s) 254, 540
TaiI ACGT 6 cut(s) 312, 363, 447, 603, 657, 729
TaqI TCGA 2 cut(s) 675, 1030
TasI AATT 4 cut(s) 139, 644, 670, 829
TauI GCSGC 4 cut(s) 95, 158, 342, 467
TfiI GAWTC 2 cut(s) 217, 1017
Tru1I TTAA 3 cut(s) 576, 669, 788
Tru9I TTAA 3 cut(s) 576, 669, 788
TscAI CASTG 2 cut(s) 423, 588
TseFI GTSAC 3 cut(s) 718, 905, 932
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 3 cut(s) 718, 905, 932
TspDTI ATGAA 8 cut(s) 512, 532, 560, 763, 791, 819, 839, 915
TspGWI ACGGA 3 cut(s) 320, 647, 815
TspRI CASTG 2 cut(s) 423, 588
VspI ATTAAT 1 cut(s) 669
XmiI GTMKAC 1 cut(s) 229
XspI CTAG 3 cut(s) 104, 404, 756
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.