Rroxscaffold_6G00410220

caffeic acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011057
Physical Location & Seq
Forward (+)
32643089 .. 32653785
10697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410220.1

Sequence Viewer

Length: 846 bp
ATGCTCACCAGCTACGGTGTGTTCTCCGAGCACTTCAACGCCGACGGCTCCGAGAGAAAGTTCTCCCTCACCGAGGTCGGCCAAACCCTAGTCACCAATCAAGATGGTCTCTCCTATGGGGCCTATGTCCTCCAGCACCACCAGGATGTACTAATGGGGGCGTGGCCACTGGTGCATGAGGCGGTGGTGGATCCCACGGTGGAGCCGTTCGTGAAGGCGAACGGCGAGCCGGCGTACCAGTACTACGGGAAGAGGCCGGAGATGAATGGGTTGATGCAAAAGGCAATGTCGGGAGTATCTGTGCCGTTCATCAAGGCAGTACTAGACGGTTACGATGGTTTTCAAGGGGTGAAGAGACTGGTGGATGTGGGTGGCAGTGCGGGGGATTGCCTGAGAATGATCCTAGACAAATATCCCAATGTACGGGAAGGCATTAACTTTGACCTACCCGAGGTCGTTGCCAAGGCCCCAACTATAGCCGGAGTGACTCATGTTGGTGGTGACATGTTCAAGTCAATTCCTAGTGGTGATGTTATCTTCATGAAGTGGATTTTATCAACATGGACGGATAGTGAGTGCAAGCTGATACTGGAGAATTGTTACAAGGCACTTCCTGCAGGAGGGAAATTGATTGCTTGTGAGCCGGTGGTGCCGAAGAAATCAGACGACAGCCACAGGACGCGTGCTCTTTTAGAGAATGACATATTCGTGATGACTATATATCGTGCCAAGGGCAAGAATAGGACTGAAGAAGAATTCCAGCAGCTTGGTCACTCCGCTGGCTTCTCTCATTTCCGACCAATCTATATTGATTACTTTTATACCGTCTTAGAATTCCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

281

Amino Acids

31.29

Weight (kDa)

5.77

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 59 - 263 5.9e-48 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53140
fragaria_vesca FvH4_6g17130 FvH4_6g17130 FvH4_6g17130
malus_domestica MD12G1103500.v1.1 MD14G1097900.v1.1
prunus_persica Prupe.7G123600_v2.0.a1
pyrus_communis pycom12g10200
rosa_chinensis RchiOBHm_Chr3g0471211
rosa_laevigata RLG00000024197
rosa_multiflora Rmu_co8165034.1_g000001 Rmu_sc0010073.1_g000018 Rmu_sc0031310.1_g000002
rosa_roxburghii Rroxscaffold_6G00410220
rosa_rugosa Rorug03G0116200
rosa_samantha Rh3AG165100 Rh3BG190700 Rh3CG181000 Rh3DG185100
rosa_wichuraiana Rw3G015570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 649
AccII CGCG 1 cut(s) 682
AciI CCGC 3 cut(s) 182, 380, 777
AclWI GGATC 3 cut(s) 185, 198, 394
AcoI YGGCCR 2 cut(s) 79, 164
AcsI RAATTY 2 cut(s) 755, 833
AcuI CTGAAG 1 cut(s) 768
AfaI GTAC 5 cut(s) 150, 236, 242, 321, 423
AfiI CCNNNNNNNGG 4 cut(s) 73, 423, 451, 620
AflIII ACRYGT 2 cut(s) 504, 680
AgsI TTSAA 3 cut(s) 37, 344, 511
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 3 cut(s) 12, 583, 766
AluI AGCT 3 cut(s) 12, 583, 766
Alw21I GWGCWC 2 cut(s) 33, 688
Alw26I GTCTC 2 cut(s) 113, 349
AlwI GGATC 3 cut(s) 185, 198, 394
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 5 cut(s) 79, 120, 164, 254, 465
ApeKI GCWGC 1 cut(s) 763
ApoI RAATTY 2 cut(s) 755, 833
AspS9I GGNCC 2 cut(s) 120, 466
AsuHPI GGTGA 5 cut(s) 61, 85, 361, 512, 539
AvaI CYCGRG 1 cut(s) 449
BalI TGGCCA 1 cut(s) 166
BamHI GGATCC 1 cut(s) 190
BanI GGYRCC 1 cut(s) 649
Bbv12I GWGCWC 2 cut(s) 33, 688
BbvI GCAGC 1 cut(s) 775
BccI CCATC 2 cut(s) 98, 329
BceAI ACGGC 4 cut(s) 61, 190, 238, 289
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 113, 349
BfaI CTAG 4 cut(s) 89, 323, 404, 522
BfmI CTRYAG 2 cut(s) 474, 615
BisI GCNGC 1 cut(s) 764
BlsI GCNGC 1 cut(s) 765
BmcAI AGTACT 2 cut(s) 242, 321
Bme1390I CCNGG 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 120, 466
BmiI GGNNCC 6 cut(s) 49, 121, 192, 204, 468, 651
BmrFI CCNGG 1 cut(s) 143
BmsI GCATC 1 cut(s) 264
BpmI CTGGAG 2 cut(s) 116, 611
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 5 cut(s) 72, 195, 450, 462, 729
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 423, 451, 620
Bse118I RCCGGY 2 cut(s) 229, 643
Bse1I ACTGG 4 cut(s) 174, 238, 363, 594
Bse3DI GCAATG 1 cut(s) 291
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 5 cut(s) 72, 195, 450, 462, 729
BseGI GGATG 2 cut(s) 151, 370
BseLI CCNNNNNNNGG 4 cut(s) 73, 423, 451, 620
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 4 cut(s) 174, 238, 363, 594
BseXI GCAGC 1 cut(s) 775
Bsh1236I CGCG 1 cut(s) 682
BshFI GGCC 5 cut(s) 81, 122, 166, 256, 467
BshNI GGYRCC 1 cut(s) 649
BsiHKAI GWGCWC 2 cut(s) 33, 688
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 4 cut(s) 230, 257, 480, 644
BslI CCNNNNNNNGG 4 cut(s) 73, 423, 451, 620
BsmAI GTCTC 2 cut(s) 113, 349
BsnI GGCC 5 cut(s) 81, 122, 166, 256, 467
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 449
Bsp1286I GDGCHC 2 cut(s) 33, 688
Bsp143I GATC 2 cut(s) 190, 399
BspACI CCGC 3 cut(s) 182, 380, 777
BspANI GGCC 5 cut(s) 81, 122, 166, 256, 467
BspCNI CTCAG 1 cut(s) 384
BspFNI CGCG 1 cut(s) 682
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 6 cut(s) 49, 121, 192, 204, 468, 651
BspMAI CTGCAG 1 cut(s) 619
BspPI GGATC 3 cut(s) 185, 198, 394
BspT107I GGYRCC 1 cut(s) 649
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 2 cut(s) 229, 643
BsrI ACTGG 4 cut(s) 174, 238, 363, 594
BssAI RCCGGY 2 cut(s) 229, 643
BssECI CCNNGG 5 cut(s) 72, 195, 450, 462, 729
BssMI GATC 2 cut(s) 190, 399
BssT1I CCWWGG 2 cut(s) 462, 729
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 4 cut(s) 17, 199, 329, 826
Bst6I CTCTTC 2 cut(s) 245, 347
BstAPI GCANNNNNTGC 1 cut(s) 614
BstC8I GCNNGC 5 cut(s) 227, 231, 581, 684, 781
BstDEI CTNAG 2 cut(s) 392, 829
BstDSI CCRYGG 1 cut(s) 195
BstENI CCTNNNNNAGG 3 cut(s) 71, 449, 618
BstF5I GGATG 2 cut(s) 151, 370
BstFNI CGCG 1 cut(s) 682
BstKTI GATC 2 cut(s) 193, 402
BstMAI GTCTC 2 cut(s) 113, 349
BstMBI GATC 2 cut(s) 190, 399
BstMWI GCNNNNNNNGC 3 cut(s) 172, 614, 649
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 2 cut(s) 474, 615
BstUI CGCG 1 cut(s) 682
BstV1I GCAGC 1 cut(s) 775
BstX2I RGATCY 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 5 cut(s) 81, 122, 166, 256, 467
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 2 cut(s) 151, 370
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 167, 382
Cac8I GCNNGC 5 cut(s) 227, 231, 581, 684, 781
CciI TCATGA 1 cut(s) 540
Cfr10I RCCGGY 2 cut(s) 229, 643
Cfr13I GGNCC 2 cut(s) 120, 466
CseI GACGC 1 cut(s) 688
Csp6I GTAC 5 cut(s) 149, 235, 241, 320, 422
CviAII CATG 5 cut(s) 176, 491, 505, 541, 561
CviQI GTAC 5 cut(s) 149, 235, 241, 320, 422
DdeI CTNAG 2 cut(s) 392, 829
DpnI GATC 2 cut(s) 192, 401
DpnII GATC 2 cut(s) 190, 399
EaeI YGGCCR 2 cut(s) 79, 164
Eam1104I CTCTTC 2 cut(s) 245, 347
EarI CTCTTC 2 cut(s) 245, 347
Eco130I CCWWGG 2 cut(s) 462, 729
Eco31I GGTCTC 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 768
Eco88I CYCGRG 1 cut(s) 449
EcoNI CCTNNNNNAGG 3 cut(s) 71, 449, 618
EcoO109I RGGNCCY 2 cut(s) 120, 466
EcoRI GAATTC 2 cut(s) 755, 833
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 2 cut(s) 462, 729
ErhI CCWWGG 2 cut(s) 462, 729
FaeI CATG 5 cut(s) 179, 494, 508, 544, 564
FatI CATG 5 cut(s) 175, 490, 504, 540, 560
FauI CCCGC 1 cut(s) 373
Fnu4HI GCNGC 1 cut(s) 764
FokI GGATG 2 cut(s) 158, 377
Fsp4HI GCNGC 1 cut(s) 764
FspBI CTAG 4 cut(s) 89, 323, 404, 522
GluI GCNGC 1 cut(s) 764
GsuI CTGGAG 2 cut(s) 116, 611
HaeIII GGCC 5 cut(s) 81, 122, 166, 256, 467
HapII CCGG 4 cut(s) 230, 257, 480, 644
HgaI GACGC 1 cut(s) 688
Hin1II CATG 5 cut(s) 179, 494, 508, 544, 564
HinfI GANTC 1 cut(s) 487
HpaII CCGG 4 cut(s) 230, 257, 480, 644
HphI GGTGA 5 cut(s) 61, 85, 361, 512, 539
Hpy188I TCNGA 4 cut(s) 28, 52, 664, 797
Hpy188III TCNNGA 5 cut(s) 101, 211, 291, 541, 709
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 2 cut(s) 208, 422
HpyCH4III ACNGT 4 cut(s) 17, 199, 329, 826
HpyCH4V TGCA 4 cut(s) 175, 277, 579, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 614, 649
HpyF3I CTNAG 2 cut(s) 392, 829
Hsp92II CATG 5 cut(s) 179, 494, 508, 544, 564
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Kzo9I GATC 2 cut(s) 190, 399
LmnI GCTCC 2 cut(s) 53, 202
Lsp1109I GCAGC 1 cut(s) 775
LweI GCATC 1 cut(s) 264
MaeI CTAG 4 cut(s) 89, 323, 404, 522
MaeIII GTNAC 6 cut(s) 91, 329, 484, 500, 599, 770
MalI GATC 2 cut(s) 192, 401
MboI GATC 2 cut(s) 190, 399
MboII GAAGA 6 cut(s) 262, 364, 529, 667, 761, 764
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 2 cut(s) 33, 688
MlsI TGGCCA 1 cut(s) 166
MluCI AATT 5 cut(s) 516, 595, 626, 755, 833
MluI ACGCGT 1 cut(s) 680
MluNI TGGCCA 1 cut(s) 166
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 1 cut(s) 820
MnlI CCTC 7 cut(s) 67, 77, 140, 172, 246, 445, 614
Mox20I TGGCCA 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 229
MscI TGGCCA 1 cut(s) 166
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 3 cut(s) 144, 495, 707
Msp20I TGGCCA 1 cut(s) 166
MspA1I CMGCKG 1 cut(s) 779
MspI CCGG 4 cut(s) 230, 257, 480, 644
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 682
MwoI GCNNNNNNNGC 3 cut(s) 172, 614, 649
NaeI GCCGGC 1 cut(s) 231
NdeII GATC 2 cut(s) 190, 399
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 5 cut(s) 179, 494, 508, 544, 564
NlaIV GGNNCC 6 cut(s) 49, 121, 192, 204, 468, 651
NmuCI GTSAC 4 cut(s) 91, 484, 500, 770
NspI RCATGY 1 cut(s) 508
PagI TCATGA 1 cut(s) 540
PciI ACATGT 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PdiI GCCGGC 1 cut(s) 231
PkrI GCNGC 1 cut(s) 765
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PscI ACATGT 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 6 cut(s) 49, 121, 192, 204, 468, 651
PspPI GGNCC 2 cut(s) 120, 466
PstI CTGCAG 1 cut(s) 619
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 5 cut(s) 150, 236, 242, 321, 423
RsaNI GTAC 5 cut(s) 149, 235, 241, 320, 422
RseI CAYNNNNRTG 3 cut(s) 144, 495, 707
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 1 cut(s) 764
Sau3AI GATC 2 cut(s) 190, 399
Sau96I GGNCC 2 cut(s) 120, 466
SbfI CCTGCAGG 1 cut(s) 619
ScaI AGTACT 2 cut(s) 242, 321
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 1 cut(s) 143
SdaI CCTGCAGG 1 cut(s) 619
SduI GDGCHC 2 cut(s) 33, 688
SetI ASST 6 cut(s) 14, 78, 447, 456, 585, 768
SfaNI GCATC 1 cut(s) 264
SfcI CTRYAG 2 cut(s) 474, 615
SmiMI CAYNNNNRTG 3 cut(s) 144, 495, 707
Sse8387I CCTGCAGG 1 cut(s) 619
Sse9I AATT 5 cut(s) 516, 595, 626, 755, 833
SsiI CCGC 3 cut(s) 182, 380, 777
SspMI CTAG 4 cut(s) 89, 323, 404, 522
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 2 cut(s) 462, 729
TaaI ACNGT 4 cut(s) 17, 199, 329, 826
TasI AATT 5 cut(s) 516, 595, 626, 755, 833
TatI WGTACW 3 cut(s) 148, 240, 319
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 2 cut(s) 174, 382
TseFI GTSAC 4 cut(s) 91, 484, 500, 770
TseI GCWGC 1 cut(s) 763
Tsp45I GTSAC 4 cut(s) 91, 484, 500, 770
TspDTI ATGAA 4 cut(s) 278, 298, 529, 557
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 2 cut(s) 174, 382
XagI CCTNNNNNAGG 3 cut(s) 71, 449, 618
XapI RAATTY 2 cut(s) 755, 833
XceI RCATGY 1 cut(s) 508
XspI CTAG 4 cut(s) 89, 323, 404, 522
ZrmI AGTACT 2 cut(s) 242, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.