FvH4_6g17130

caffeic acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011057
Physical Location & Seq
Reverse (-)
11122823 .. 11125983
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17130.t3

Sequence Viewer

Length: 1077 bp
ATGGAAGACGCAAACAGAATCAAAGAGGCAAGCAACGAAGCAAGGATTGCCATACTGGAGCTTGCCAACATGATCAGCGTCCCTATGTCCCTAAACGCCGTCGTCCGTCTCAACGTCGCCGACGCCATCTGGCAAAAGGGTTCCAACACTCCCCTCTCTGCCTCTCAGATCCTCGAACGTGTTCTCCCCACCGGCAGTGGTGACGCCGAGAACCTCCAGCGCATCCTCCGCATGCTCACCAGCTACGGTGTGTTCTCCGAGCACTTCAACGCCGATGGCTCCGAGAGAAAGTTCTCTCTCACTGAGGTCGGGCAAACCCTAGTCACCAATCAAGATGGCCTCTCCTATGGGGACTATGTCCTCCAGCACCACCAGGATGTGCTAATGGGGGCATGGCCGCTGGTGCACGAGGCGGTGATTGACCCCTCGGTGGAGCCGTTTGTGAAGGCAAACGGCGAGCCGGCGTACCAGTACTACGGGAAGAGGCCGGAGATGAACGGGTTGATGCAGAAGGCAATGTCGGGAGTATCTGTGCCGTTCATAAAGGCGGTATTAGACGGCTACGATGGTTTTCAGGGGGTGACGAGACTGGTGGATGTGGGTGGGAGTGCCGGAGATTGCCTGAAAATGATCATGGACAGATATCCTAATGTAAGGGAAGGCATTAACTTTGATCTACCTGAGGTAGTTGCCAAGGCCCCAACTATTGCCGGAGTGACTCATGTTGGTGGTGACATGTTCAAGTCTATTCCTAGTGCTGATGTTATCTTCATGAAGTGGATTTTATCAACATGGACAGATTGCGAGTGCAAGCTGATACTGGAGAACTGTTACAAGGCACTTCCTGCAGGAGGAAAACTGATCGCTTGTGAGCCGGTGGTTCCGAGAAAATCAGATGACAGCCATAGAACTCGTGCTCTTTTAGAGAATGACATATTCGTGATGACTATATATCGCACCAAGGGCAAGAACAGGACCGAAGAAGAACTCCGGCAGCTCGGTCACTCCGCCGGCTTCTCTCATTTCCGACCAATCTATATTGATTACTTTTATACCGTCTTAGAATTCCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

359

Amino Acids

39.78

Weight (kDa)

5.57

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 19 - 109 2.9e-16 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 136 - 263 7.4e-26 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53140
fragaria_vesca FvH4_6g17130 FvH4_6g17130 FvH4_6g17130
malus_domestica MD12G1103500.v1.1 MD14G1097900.v1.1
prunus_persica Prupe.7G123600_v2.0.a1
pyrus_communis pycom12g10200
rosa_chinensis RchiOBHm_Chr3g0471211
rosa_laevigata RLG00000024197
rosa_multiflora Rmu_co8165034.1_g000001 Rmu_sc0010073.1_g000018 Rmu_sc0031310.1_g000002
rosa_roxburghii Rroxscaffold_6G00410220
rosa_rugosa Rorug03G0116200
rosa_samantha Rh3AG165100 Rh3BG190700 Rh3CG181000 Rh3DG185100
rosa_wichuraiana Rw3G015570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 229, 398, 413, 548, 1008
AclWI GGATC 1 cut(s) 163
AcoI YGGCCR 1 cut(s) 395
AcsI RAATTY 1 cut(s) 1064
AcyI GRCGYC 2 cut(s) 123, 204
AfaI GTAC 2 cut(s) 467, 473
AfiI CCNNNNNNNGG 2 cut(s) 430, 851
AflIII ACRYGT 2 cut(s) 178, 735
AgsI TTSAA 2 cut(s) 268, 742
AjnI CCWGG 1 cut(s) 372
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 4 cut(s) 61, 243, 814, 997
AluI AGCT 4 cut(s) 61, 243, 814, 997
Alw21I GWGCWC 3 cut(s) 264, 408, 919
Alw26I GTCTC 2 cut(s) 113, 580
Alw44I GTGCAC 1 cut(s) 404
AlwI GGATC 1 cut(s) 163
AoxI GGCC 4 cut(s) 337, 395, 485, 696
ApaLI GTGCAC 1 cut(s) 404
ApeKI GCWGC 1 cut(s) 994
ApoI RAATTY 1 cut(s) 1064
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 697, 975
AsuHPI GGTGA 6 cut(s) 212, 229, 316, 427, 592, 743
AvaII GGWCC 1 cut(s) 975
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 1 cut(s) 408
BauI CACGAG 2 cut(s) 407, 912
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 3 cut(s) 264, 408, 919
BbvI GCAGC 1 cut(s) 1006
BccI CCATC 4 cut(s) 134, 269, 329, 560
BceAI ACGGC 5 cut(s) 83, 421, 469, 520, 574
BciT130I CCWGG 1 cut(s) 374
BclI TGATCA 2 cut(s) 72, 630
BcoDI GTCTC 2 cut(s) 113, 580
BfaI CTAG 2 cut(s) 320, 753
BfmI CTRYAG 1 cut(s) 846
BisI GCNGC 2 cut(s) 398, 995
BlsI GCNGC 2 cut(s) 399, 996
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 1 cut(s) 374
Bme18I GGWCC 1 cut(s) 975
BmgT120I GGNCC 2 cut(s) 697, 975
BmiI GGNNCC 5 cut(s) 142, 280, 435, 699, 882
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 2 cut(s) 231, 495
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 4 cut(s) 77, 200, 347, 842
BsaHI GRCGYC 2 cut(s) 123, 204
BsaJI CCNNGG 3 cut(s) 426, 693, 960
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 516, 546
Bsc4I CCNNNNNNNGG 2 cut(s) 430, 851
Bse118I RCCGGY 4 cut(s) 191, 460, 874, 1010
Bse1I ACTGG 4 cut(s) 60, 469, 594, 825
Bse21I CCTNAGG 1 cut(s) 681
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 426, 693, 960
BseGI GGATG 3 cut(s) 222, 382, 601
BseLI CCNNNNNNNGG 2 cut(s) 430, 851
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 3 cut(s) 179, 294, 672
BseNI ACTGG 4 cut(s) 60, 469, 594, 825
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 1 cut(s) 1006
BshFI GGCC 4 cut(s) 339, 397, 487, 698
BsiHKAI GWGCWC 3 cut(s) 264, 408, 919
BsiSI CCGG 8 cut(s) 192, 461, 488, 612, 711, 875, 991, 1011
BslFI GGGAC 3 cut(s) 65, 73, 365
BslI CCNNNNNNNGG 2 cut(s) 430, 851
BsmAI GTCTC 2 cut(s) 113, 580
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 3 cut(s) 65, 73, 365
BsnI GGCC 4 cut(s) 339, 397, 487, 698
Bsp1286I GDGCHC 3 cut(s) 264, 408, 919
Bsp143I GATC 5 cut(s) 72, 168, 630, 673, 861
BspACI CCGC 5 cut(s) 229, 398, 413, 548, 1008
BspANI GGCC 4 cut(s) 339, 397, 487, 698
BspCNI CTCAG 3 cut(s) 178, 295, 673
BspHI TCATGA 1 cut(s) 771
BspLI GGNNCC 5 cut(s) 142, 280, 435, 699, 882
BspMAI CTGCAG 1 cut(s) 850
BspPI GGATC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 4 cut(s) 191, 460, 874, 1010
BsrI ACTGG 4 cut(s) 60, 469, 594, 825
BssAI RCCGGY 4 cut(s) 191, 460, 874, 1010
BssECI CCNNGG 3 cut(s) 426, 693, 960
BssMI GATC 5 cut(s) 72, 168, 630, 673, 861
BssNI GRCGYC 2 cut(s) 123, 204
BssSI CACGAG 2 cut(s) 407, 912
BssT1I CCWWGG 2 cut(s) 693, 960
Bst2BI CACGAG 2 cut(s) 407, 912
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 3 cut(s) 248, 830, 1057
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 2 cut(s) 123, 204
BstAPI GCANNNNNTGC 2 cut(s) 47, 845
BstC8I GCNNGC 7 cut(s) 31, 63, 233, 458, 462, 812, 1012
BstDEI CTNAG 4 cut(s) 165, 303, 681, 1060
BstENI CCTNNNNNAGG 1 cut(s) 849
BstF5I GGATG 3 cut(s) 222, 382, 601
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 5 cut(s) 75, 171, 633, 676, 864
BstMAI GTCTC 2 cut(s) 113, 580
BstMBI GATC 5 cut(s) 72, 168, 630, 673, 861
BstMWI GCNNNNNNNGC 5 cut(s) 47, 228, 403, 845, 963
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 2 cut(s) 235, 739
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 1006
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 4 cut(s) 339, 397, 487, 698
BtsCI GGATG 3 cut(s) 222, 382, 601
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 202, 300
Cac8I GCNNGC 7 cut(s) 31, 63, 233, 458, 462, 812, 1012
CciI TCATGA 1 cut(s) 771
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 4 cut(s) 191, 460, 874, 1010
Cfr13I GGNCC 2 cut(s) 697, 975
CseI GACGC 4 cut(s) 17, 67, 131, 212
Csp6I GTAC 2 cut(s) 466, 472
CviAII CATG 8 cut(s) 70, 232, 393, 634, 722, 736, 772, 792
CviQI GTAC 2 cut(s) 466, 472
DdeI CTNAG 4 cut(s) 165, 303, 681, 1060
DpnI GATC 5 cut(s) 74, 170, 632, 675, 863
DpnII GATC 5 cut(s) 72, 168, 630, 673, 861
EaeI YGGCCR 1 cut(s) 395
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
EciI GGCGGA 1 cut(s) 997
Eco130I CCWWGG 2 cut(s) 693, 960
Eco32I GATATC 1 cut(s) 644
Eco47I GGWCC 1 cut(s) 975
Eco81I CCTNAGG 1 cut(s) 681
EcoNI CCTNNNNNAGG 1 cut(s) 849
EcoO109I RGGNCCY 1 cut(s) 697
EcoRI GAATTC 1 cut(s) 1064
EcoRII CCWGG 1 cut(s) 372
EcoRV GATATC 1 cut(s) 644
EcoT14I CCWWGG 2 cut(s) 693, 960
ErhI CCWWGG 2 cut(s) 693, 960
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 8 cut(s) 73, 235, 396, 637, 725, 739, 775, 795
FaqI GGGAC 3 cut(s) 65, 73, 365
FatI CATG 8 cut(s) 69, 231, 392, 633, 721, 735, 771, 791
FbaI TGATCA 2 cut(s) 72, 630
Fnu4HI GCNGC 2 cut(s) 398, 995
FokI GGATG 3 cut(s) 209, 389, 608
Fsp4HI GCNGC 2 cut(s) 398, 995
FspBI CTAG 2 cut(s) 320, 753
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 398, 995
GsuI CTGGAG 4 cut(s) 77, 200, 347, 842
HaeIII GGCC 4 cut(s) 339, 397, 487, 698
HapII CCGG 8 cut(s) 192, 461, 488, 612, 711, 875, 991, 1011
HgaI GACGC 4 cut(s) 17, 67, 131, 212
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 2 cut(s) 123, 204
Hin1II CATG 8 cut(s) 73, 235, 396, 637, 725, 739, 775, 795
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 2 cut(s) 18, 718
HpaII CCGG 8 cut(s) 192, 461, 488, 612, 711, 875, 991, 1011
HphI GGTGA 6 cut(s) 212, 229, 316, 427, 592, 743
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 6 cut(s) 168, 259, 283, 885, 895, 1028
Hpy188III TCNNGA 4 cut(s) 332, 522, 772, 940
Hpy8I GTNNAC 1 cut(s) 406
Hpy99I CGWCG 3 cut(s) 104, 119, 125
HpyAV CCTTC 3 cut(s) 439, 505, 653
HpyCH4III ACNGT 3 cut(s) 248, 830, 1057
HpyCH4IV ACGT 2 cut(s) 114, 178
HpyCH4V TGCA 4 cut(s) 406, 508, 810, 848
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 228, 403, 845, 963
HpyF3I CTNAG 4 cut(s) 165, 303, 681, 1060
HpySE526I ACGT 2 cut(s) 114, 178
Hsp92I GRCGYC 2 cut(s) 123, 204
Hsp92II CATG 8 cut(s) 73, 235, 396, 637, 725, 739, 775, 795
HspAI GCGC 1 cut(s) 220
KroI GCCGGC 2 cut(s) 460, 1010
KroNI GCCGGC 2 cut(s) 462, 1012
Ksp22I TGATCA 2 cut(s) 72, 630
Kzo9I GATC 5 cut(s) 72, 168, 630, 673, 861
LmnI GCTCC 3 cut(s) 58, 284, 433
Lsp1109I GCAGC 1 cut(s) 1006
LweI GCATC 2 cut(s) 231, 495
MaeI CTAG 2 cut(s) 320, 753
MaeII ACGT 2 cut(s) 114, 178
MaeIII GTNAC 7 cut(s) 200, 322, 580, 715, 731, 830, 1001
MalI GATC 5 cut(s) 74, 170, 632, 675, 863
MboI GATC 5 cut(s) 72, 168, 630, 673, 861
MboII GAAGA 5 cut(s) 17, 493, 760, 992, 995
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 3 cut(s) 264, 408, 919
MluCI AATT 1 cut(s) 1064
MlyI GAGTC 1 cut(s) 712
MmeI TCCRAC 2 cut(s) 168, 1051
MroNI GCCGGC 2 cut(s) 460, 1010
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 1 cut(s) 666
MslI CAYNNNNRTG 3 cut(s) 375, 726, 938
MspA1I CMGCKG 1 cut(s) 400
MspI CCGG 8 cut(s) 192, 461, 488, 612, 711, 875, 991, 1011
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 5 cut(s) 47, 228, 403, 845, 963
NaeI GCCGGC 2 cut(s) 462, 1012
NdeII GATC 5 cut(s) 72, 168, 630, 673, 861
NgoMIV GCCGGC 2 cut(s) 460, 1010
NlaIII CATG 8 cut(s) 73, 235, 396, 637, 725, 739, 775, 795
NlaIV GGNNCC 5 cut(s) 142, 280, 435, 699, 882
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 6 cut(s) 200, 322, 580, 715, 731, 1001
NspI RCATGY 2 cut(s) 235, 739
PaeI GCATGC 1 cut(s) 235
PagI TCATGA 1 cut(s) 771
PciI ACATGT 1 cut(s) 735
PcsI WCGNNNNNNNCGW 1 cut(s) 434
PdiI GCCGGC 2 cut(s) 462, 1012
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 1 cut(s) 18
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 2 cut(s) 399, 996
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
PscI ACATGT 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 5 cut(s) 142, 280, 435, 699, 882
PspPI GGNCC 2 cut(s) 697, 975
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 168
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 2 cut(s) 467, 473
RsaNI GTAC 2 cut(s) 466, 472
RseI CAYNNNNRTG 3 cut(s) 375, 726, 938
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 2 cut(s) 398, 995
Sau3AI GATC 5 cut(s) 72, 168, 630, 673, 861
Sau96I GGNCC 2 cut(s) 697, 975
SbfI CCTGCAGG 1 cut(s) 850
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 1 cut(s) 712
ScrFI CCNGG 1 cut(s) 374
SdaI CCTGCAGG 1 cut(s) 850
SduI GDGCHC 3 cut(s) 264, 408, 919
SfaNI GCATC 2 cut(s) 231, 495
SfcI CTRYAG 1 cut(s) 846
SinI GGWCC 1 cut(s) 975
SmiMI CAYNNNNRTG 3 cut(s) 375, 726, 938
SphI GCATGC 1 cut(s) 235
Sse8387I CCTGCAGG 1 cut(s) 850
Sse9I AATT 1 cut(s) 1064
SsiI CCGC 5 cut(s) 229, 398, 413, 548, 1008
SspMI CTAG 2 cut(s) 320, 753
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 2 cut(s) 693, 960
TaaI ACNGT 3 cut(s) 248, 830, 1057
TaiI ACGT 2 cut(s) 117, 181
TaqI TCGA 1 cut(s) 174
TaqII GACCGA 2 cut(s) 989, 992
TasI AATT 1 cut(s) 1064
TatI WGTACW 1 cut(s) 471
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 2 cut(s) 202, 307
TseFI GTSAC 6 cut(s) 200, 322, 580, 715, 731, 1001
TseI GCWGC 1 cut(s) 994
Tsp45I GTSAC 6 cut(s) 200, 322, 580, 715, 731, 1001
TspDTI ATGAA 4 cut(s) 509, 529, 760, 788
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 2 cut(s) 202, 307
Tth111I GACNNNGTC 1 cut(s) 356
VneI GTGCAC 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 975
XagI CCTNNNNNAGG 1 cut(s) 849
XapI RAATTY 1 cut(s) 1064
XceI RCATGY 2 cut(s) 235, 739
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 2 cut(s) 320, 753
ZrmI AGTACT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.