AT3G54000

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011807
Physical Location & Seq
Forward (+)
19996177 .. 19998736
2560 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54000.2

Sequence Viewer

Length: 1059 bp
ATGGAACCTCCTAGTAGCGTAGTCGACGATGCTGAGTTTTGGCTTCCGACGGAGTTTCTCACTGACGATGATTTTCTCGTTGAGAAGGAGAATAACAGTGTAGGAATCGATGACTCCTTGTTCCCGTACGAGCCTCGTCATGGATTTGGGACTTTTGGCTCTACCGTTAAGCCTAACACGGTGAAGGAAGATGACGAGGAGAGTTTCTTAGCCGGATTGACTCGGCAGATGGTTATGTCATCTCTCAAAGATGATTTCTCCGGTGGAGTTTGCGGTAATCACGCTTTTCCGGCCGGTAATGACCACAAGGCGTGGGAAATGAATCGTTCGCCTCCGTGTGTCGCCGGAACTGGCTGTTGTTGCTTGAATCAGCGGTTTAACCAGAACCTTAATTCGCGAGTTTCTTCATGGGATCTGTACTGTGCTGCGGAGAGGATGAGTATCAACGACGAACCATACCATAGTGGCAGAGGACTTCTCGGATCTCCCGCAAAACTTTCCGCTACCGTCAAAAACCACTCTAACAACGGCACCGGTTACTACAACAATCATCAATCTCTCCAATACCAGAAGCTACAAGCTATCCAGTTTCAGCAGCTAAAGCAGCAACAGTTAATGAAACATCGTCGCCAATTGGTGCGTCAGAACAGAGGAGTAAGAGTTAATGGCAACAAAAATGTTGGTCCAGTGGATTTGTCGTCTTCTGCATGGTCTAATCAGTTTCCGAGGCGTGATGTTATGAGAGCTGTATTCATCGGGGACCATACCGGAAAACGTGGCTCAACCGGAACCGGTGTCTTCTTACCTCGAAGTGTTAACCATACTTCAAGAACTGAGACTCGAGAGAAGCCTACTATATCGACGGTTTTGGTTCCGGCTAGACTGGCTCAGGTCCTGAATCTGAATTTAGGCGAACCGGTCCGGTCTACGGCTACTCTCAACGATGTGTCGTGGAGACAGAGGAGCAACAATGGTGGGTTCTCGAGCCAGATGGTCGGTGGCGTGAGGGCGGAGCAGTCAGTGCAAGAGCCTCGGTTACCATCGGAGTGGGCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

352

Amino Acids

39.18

Weight (kDa)

8.19

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54000 AT3G54000 AT3G54000
fragaria_vesca FvH4_6g12650
malus_domestica MD04G1140200.v1.1 MD12G1155400.v1.1 MD15G1149300.v1.1
prunus_persica Prupe.6G265300_v2.0.a1
pyrus_communis pycom04g12780 pycom12g14750
rosa_chinensis RchiOBHm_Chr3g0464501
rosa_laevigata RLG00000024691
rosa_multiflora Rmu_sc0003074.1_g000005
rosa_roxburghii Rroxscaffold_6G00415610
rosa_rugosa Rorug03G0072700
rosa_samantha Rh3BG134900 Rh3CG137400 Rh3DG136200
rosa_wichuraiana Rw3G011030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 530
AccI GTMKAC 2 cut(s) 24, 926
AccII CGCG 1 cut(s) 397
AciI CCGC 6 cut(s) 273, 373, 428, 489, 501, 1010
AclWI GGATC 2 cut(s) 420, 490
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 904
AfaI GTAC 2 cut(s) 128, 419
AfiI CCNNNNNNNGG 2 cut(s) 140, 928
AgeI ACCGGT 3 cut(s) 533, 791, 916
AgsI TTSAA 2 cut(s) 367, 828
AluBI AGCT 4 cut(s) 574, 581, 598, 746
AluI AGCT 4 cut(s) 574, 581, 598, 746
Alw26I GTCTC 2 cut(s) 830, 949
AlwI GGATC 2 cut(s) 420, 490
AlwNI CAGNNNCTG 1 cut(s) 895
Ama87I CYCGRG 2 cut(s) 840, 982
AoxI GGCC 1 cut(s) 291
ApeKI GCWGC 3 cut(s) 425, 595, 604
ApoI RAATTY 1 cut(s) 904
AsiGI ACCGGT 3 cut(s) 533, 791, 916
AspS9I GGNCC 4 cut(s) 683, 760, 892, 919
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 2 cut(s) 840, 982
AvaII GGWCC 4 cut(s) 683, 760, 892, 919
BanI GGYRCC 1 cut(s) 530
BbsI GAAGAC 2 cut(s) 693, 790
BbvI GCAGC 3 cut(s) 412, 607, 616
BccI CCATC 3 cut(s) 223, 985, 1048
BceAI ACGGC 2 cut(s) 544, 945
BcgI CGANNNNNNTGC 2 cut(s) 1013, 1047
BcoDI GTCTC 2 cut(s) 830, 949
BfaI CTAG 2 cut(s) 12, 879
BisI GCNGC 3 cut(s) 426, 596, 605
BlsI GCNGC 3 cut(s) 427, 597, 606
Bme18I GGWCC 4 cut(s) 683, 760, 892, 919
BmeT110I CYCGRG 2 cut(s) 840, 982
BmgT120I GGNCC 4 cut(s) 683, 760, 892, 919
BmiI GGNNCC 5 cut(s) 6, 532, 761, 790, 873
BmsI GCATC 1 cut(s) 19
BpiI GAAGAC 2 cut(s) 693, 790
BplI GAGNNNNNCTC 2 cut(s) 462, 494
Bpu10I CCTNAGC 1 cut(s) 888
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 440
BsaJI CCNNGG 2 cut(s) 725, 1031
BsaWI WCCGGW 7 cut(s) 260, 533, 767, 785, 791, 916, 921
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 928
Bse118I RCCGGY 4 cut(s) 293, 533, 791, 916
Bse1I ACTGG 4 cut(s) 355, 586, 686, 888
Bse8I GATNNNNATC 1 cut(s) 440
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 725, 1031
BseGI GGATG 1 cut(s) 441
BseJI GATNNNNATC 1 cut(s) 440
BseLI CCNNNNNNNGG 2 cut(s) 140, 928
BseMII CTCAG 3 cut(s) 24, 825, 902
BseNI ACTGG 4 cut(s) 355, 586, 686, 888
BseRI GAGGAG 3 cut(s) 212, 666, 976
BseX3I CGGCCG 1 cut(s) 291
BseXI GCAGC 3 cut(s) 412, 607, 616
Bsh1236I CGCG 1 cut(s) 397
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 1 cut(s) 293
BshNI GGYRCC 1 cut(s) 530
BshTI ACCGGT 3 cut(s) 533, 791, 916
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 294
BsiHKCI CYCGRG 2 cut(s) 840, 982
BsiWI CGTACG 1 cut(s) 126
BslFI GGGAC 2 cut(s) 163, 773
BslI CCNNNNNNNGG 2 cut(s) 140, 928
BsmAI GTCTC 2 cut(s) 830, 949
BsmFI GGGAC 2 cut(s) 163, 773
BsnI GGCC 1 cut(s) 293
BsoBI CYCGRG 2 cut(s) 840, 982
Bsp143I GATC 2 cut(s) 412, 482
Bsp68I TCGCGA 1 cut(s) 397
BspACI CCGC 6 cut(s) 273, 373, 428, 489, 501, 1010
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 3 cut(s) 25, 826, 901
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 1 cut(s) 397
BspLI GGNNCC 5 cut(s) 6, 532, 761, 790, 873
BspPI GGATC 2 cut(s) 420, 490
BspT107I GGYRCC 1 cut(s) 530
BsrFI RCCGGY 4 cut(s) 293, 533, 791, 916
BsrI ACTGG 4 cut(s) 355, 586, 686, 888
BssAI RCCGGY 4 cut(s) 293, 533, 791, 916
BssECI CCNNGG 2 cut(s) 725, 1031
BssMI GATC 2 cut(s) 412, 482
Bst4CI ACNGT 7 cut(s) 98, 166, 181, 422, 508, 612, 865
BstAPI GCANNNNNTGC 1 cut(s) 1021
BstDEI CTNAG 4 cut(s) 33, 208, 834, 888
BstEII GGTNACC 1 cut(s) 1035
BstF5I GGATG 1 cut(s) 441
BstFNI CGCG 1 cut(s) 397
BstKTI GATC 2 cut(s) 415, 485
BstMAI GTCTC 2 cut(s) 830, 949
BstMBI GATC 2 cut(s) 412, 482
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 6 cut(s) 290, 360, 601, 604, 884, 1021
BstPI GGTNACC 1 cut(s) 1035
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 3 cut(s) 412, 607, 616
BstV2I GAAGAC 2 cut(s) 693, 790
BstX2I RGATCY 2 cut(s) 412, 482
BstXI CCANNNNNNTGG 1 cut(s) 1047
BstYI RGATCY 2 cut(s) 412, 482
BstZI CGGCCG 1 cut(s) 291
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 108
BtsCI GGATG 1 cut(s) 441
BtsIMutI CAGTG 4 cut(s) 60, 103, 693, 1026
BtuMI TCGCGA 1 cut(s) 397
CaiI CAGNNNCTG 1 cut(s) 895
Cfr10I RCCGGY 4 cut(s) 293, 533, 791, 916
Cfr13I GGNCC 4 cut(s) 683, 760, 892, 919
ClaI ATCGAT 1 cut(s) 108
CpoI CGGWCCG 1 cut(s) 919
CseI GACGC 1 cut(s) 629
Csp6I GTAC 2 cut(s) 127, 418
CspAI ACCGGT 3 cut(s) 533, 791, 916
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CspI CGGWCCG 1 cut(s) 919
CviAII CATG 3 cut(s) 140, 408, 708
CviQI GTAC 2 cut(s) 127, 418
DdeI CTNAG 4 cut(s) 33, 208, 834, 888
DpnI GATC 2 cut(s) 414, 484
DpnII GATC 2 cut(s) 412, 482
EaeI YGGCCR 1 cut(s) 291
EagI CGGCCG 1 cut(s) 291
EciI GGCGGA 1 cut(s) 1025
EclXI CGGCCG 1 cut(s) 291
Eco47I GGWCC 4 cut(s) 683, 760, 892, 919
Eco52I CGGCCG 1 cut(s) 291
Eco88I CYCGRG 2 cut(s) 840, 982
Eco91I GGTNACC 1 cut(s) 1035
EcoO109I RGGNCCY 1 cut(s) 892
EcoO65I GGTNACC 1 cut(s) 1035
FaeI CATG 3 cut(s) 143, 411, 711
FaqI GGGAC 2 cut(s) 163, 773
FatI CATG 3 cut(s) 139, 407, 707
FauI CCCGC 1 cut(s) 496
FblI GTMKAC 2 cut(s) 24, 926
Fnu4HI GCNGC 3 cut(s) 426, 596, 605
FokI GGATG 1 cut(s) 448
Fsp4HI GCNGC 3 cut(s) 426, 596, 605
FspBI CTAG 2 cut(s) 12, 879
GluI GCNGC 3 cut(s) 426, 596, 605
HaeIII GGCC 1 cut(s) 293
HgaI GACGC 1 cut(s) 629
Hin1II CATG 3 cut(s) 143, 411, 711
HincII GTYRAC 2 cut(s) 25, 817
HindII GTYRAC 2 cut(s) 25, 817
HinfI GANTC 7 cut(s) 105, 113, 220, 322, 367, 838, 898
HpaI GTTAAC 1 cut(s) 817
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 3 cut(s) 25, 817, 927
Hpy188I TCNGA 6 cut(s) 48, 482, 645, 726, 903, 1045
Hpy188III TCNNGA 5 cut(s) 396, 828, 842, 895, 982
Hpy8I GTNNAC 3 cut(s) 25, 817, 927
Hpy99I CGWCG 5 cut(s) 29, 52, 452, 630, 865
HpyAV CCTTC 2 cut(s) 79, 178
HpyCH4III ACNGT 7 cut(s) 98, 166, 181, 422, 508, 612, 865
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 2 cut(s) 707, 1024
HpyF10VI GCNNNNNNNGC 6 cut(s) 290, 360, 601, 604, 884, 1021
HpyF3I CTNAG 4 cut(s) 33, 208, 834, 888
HpySE526I ACGT 1 cut(s) 775
Hsp92II CATG 3 cut(s) 143, 411, 711
KspAI GTTAAC 1 cut(s) 817
Kzo9I GATC 2 cut(s) 412, 482
LmnI GCTCC 2 cut(s) 963, 1012
Lsp1109I GCAGC 3 cut(s) 412, 607, 616
LweI GCATC 1 cut(s) 19
MaeI CTAG 2 cut(s) 12, 879
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 2 cut(s) 536, 1035
MalI GATC 2 cut(s) 414, 484
MboI GATC 2 cut(s) 412, 482
MboII GAAGA 4 cut(s) 200, 396, 693, 790
MfeI CAATTG 1 cut(s) 632
MflI RGATCY 2 cut(s) 412, 482
MluCI AATT 3 cut(s) 391, 632, 904
MlyI GAGTC 3 cut(s) 107, 214, 832
MmeI TCCRAC 1 cut(s) 71
MseI TTAA 6 cut(s) 168, 378, 390, 614, 663, 816
MslI CAYNNNNRTG 1 cut(s) 1045
MspA1I CMGCKG 1 cut(s) 373
MunI CAATTG 1 cut(s) 632
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 6 cut(s) 290, 360, 601, 604, 884, 1021
NdeII GATC 2 cut(s) 412, 482
NlaIII CATG 3 cut(s) 143, 411, 711
NlaIV GGNNCC 5 cut(s) 6, 532, 761, 790, 873
NmeAIII GCCGAG 1 cut(s) 202
NruI TCGCGA 1 cut(s) 397
PaeR7I CTCGAG 2 cut(s) 840, 982
PfeI GAWTC 4 cut(s) 105, 322, 367, 898
Pfl23II CGTACG 1 cut(s) 126
PinAI ACCGGT 3 cut(s) 533, 791, 916
PkrI GCNGC 3 cut(s) 427, 597, 606
PleI GAGTC 3 cut(s) 107, 214, 832
PpsI GAGTC 3 cut(s) 107, 214, 832
PpuMI RGGWCCY 1 cut(s) 892
Psp5II RGGWCCY 1 cut(s) 892
PspEI GGTNACC 1 cut(s) 1035
PspLI CGTACG 1 cut(s) 126
PspN4I GGNNCC 5 cut(s) 6, 532, 761, 790, 873
PspPI GGNCC 4 cut(s) 683, 760, 892, 919
PspPPI RGGWCCY 1 cut(s) 892
PstNI CAGNNNCTG 1 cut(s) 895
PsuI RGATCY 2 cut(s) 412, 482
RruI TCGCGA 1 cut(s) 397
RsaI GTAC 2 cut(s) 128, 419
RsaNI GTAC 2 cut(s) 127, 418
RseI CAYNNNNRTG 1 cut(s) 1045
Rsr2I CGGWCCG 1 cut(s) 919
RsrII CGGWCCG 1 cut(s) 919
SalI GTCGAC 1 cut(s) 23
SaqAI TTAA 6 cut(s) 168, 378, 390, 614, 663, 816
SatI GCNGC 3 cut(s) 426, 596, 605
Sau3AI GATC 2 cut(s) 412, 482
Sau96I GGNCC 4 cut(s) 683, 760, 892, 919
SchI GAGTC 3 cut(s) 107, 214, 832
SetI ASST 9 cut(s) 10, 390, 576, 583, 600, 748, 778, 808, 894
SfaNI GCATC 1 cut(s) 19
Sfr274I CTCGAG 2 cut(s) 840, 982
SinI GGWCC 4 cut(s) 683, 760, 892, 919
SlaI CTCGAG 2 cut(s) 840, 982
SmiMI CAYNNNNRTG 1 cut(s) 1045
SmlI CTYRAG 2 cut(s) 840, 982
SmoI CTYRAG 2 cut(s) 840, 982
Sse9I AATT 3 cut(s) 391, 632, 904
SsiI CCGC 6 cut(s) 273, 373, 428, 489, 501, 1010
SspMI CTAG 2 cut(s) 12, 879
TaaI ACNGT 7 cut(s) 98, 166, 181, 422, 508, 612, 865
TaiI ACGT 1 cut(s) 778
TaqI TCGA 6 cut(s) 24, 108, 808, 841, 860, 983
TasI AATT 3 cut(s) 391, 632, 904
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 4 cut(s) 105, 322, 367, 898
Tru1I TTAA 6 cut(s) 168, 378, 390, 614, 663, 816
Tru9I TTAA 6 cut(s) 168, 378, 390, 614, 663, 816
TscAI CASTG 4 cut(s) 67, 103, 693, 1026
TseI GCWGC 3 cut(s) 425, 595, 604
TspDTI ATGAA 4 cut(s) 335, 396, 632, 742
TspGWI ACGGA 2 cut(s) 65, 324
TspRI CASTG 4 cut(s) 67, 103, 693, 1026
VpaK11BI GGWCC 4 cut(s) 683, 760, 892, 919
XapI RAATTY 1 cut(s) 904
XcmI CCANNNNNNNNNTGG 1 cut(s) 995
XhoI CTCGAG 2 cut(s) 840, 982
XmiI GTMKAC 2 cut(s) 24, 926
XspI CTAG 2 cut(s) 12, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.