MD12G1155400.v1.1

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011807
Physical Location & Seq
Forward (+)
23606505 .. 23609306
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1155400.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGCTGATACGTTAGATGACCTTGCGTTTTGGCTCCCCACCCAGATTCTCAACGACGAAGACCCACCCGCCATGGAGCTCAACTCCAACGCCAAGAAAAGTGGGGGGTTCGGATTACGGTTCGACGCTGATGCTCCGAAGGCTCTGTTGCCGTTTGAGTTTCCTTACGGCGGTGCTTTTGGGGTTTCGTCGGATTTCAGTTCGCCGGTCGAGTCGAGCGAGACTGAGAGCGATGAGGAAGACTACCTCACCGGGCTGACCCGCCAAATGGCTTACTCTACTCTCGACGACGAGTTCAACTTCAAGCACTCCAACTCTGCTTTCGCCTCCGAGAAGCCAAAGGGTTGGGTCGCTTCTGGTTCTCCCCAATCGACTCTCTACGCAGTCAGCAGTGGTTGCGGTTGCGGACAGGGCTCGAGCCGCGGAAGCCCAAACGCCCAGTCGCCGCCGGCCACCTGGGATCTGCTGCATGCAGCTGCAGGGGAAGTTGCCAAGATGAGCATGAACCAAAGCAGAGGCCTCCTGGATCCTCCACGAAAGCCTTCCCCCGTCTCTGTCCCCACCAACAACCCAGATGCTGCTTATTGCTTCCACCAGCAGTCGCTTTCTCACAAGCACTTGCGAGCCGCTCAGTTTAAGCTGTTGAGGCAGCAACAGATGATGAAGGAGCAGAGCGCGGCGGTCTGGGGAGCACAAACTCGATATCCTCCGCCGCAGACTCAGCCGCAGACCCACCCAAACTTCCAAACCAGAGCGAGAAACACTCTCAACATTCTCAACGCTCGGCCTGTGGGTTTGTCGCCATCTGCATGGCCGCCTCTGCAACAAGCTAAGCAGCAGCAAGAGTACAACAGAAAGGGGTCCGGATTGAGAGCTGTTTATCTCGGAAACCAATACCCATCTGCCAAAATTGAGCGTTCCGGCACCGGCGTCTTCTTACCCCGCCAAGTTGGCACCGAATCCGAAACCCGCAAGAAGCCAGTTTGCTCCACAGTTCTACTCCCAGCTCGAGTCGTGCAGGCTCTGAACCTGAACATTGATGACATGCAACATCCCCAGCTTCAGCGTCGTTTTAATGGCAAACTGAACTCTGATTACGAGGTTGCTCTTCAGCTTCGGAGCAACGCCGCCGCCAGAACCGCCATTTCGCAGCAGAAGCGCATCATTCGGCCGCAGGCGGCAGTAGGCAGCGAGATAAGGCTCCCCCAGGAGTGGACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

408

Amino Acids

44.75

Weight (kDa)

8.91

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54000 AT3G54000 AT3G54000
fragaria_vesca FvH4_6g12650
malus_domestica MD04G1140200.v1.1 MD12G1155400.v1.1 MD15G1149300.v1.1
prunus_persica Prupe.6G265300_v2.0.a1
pyrus_communis pycom04g12780 pycom12g14750
rosa_chinensis RchiOBHm_Chr3g0464501
rosa_laevigata RLG00000024691
rosa_multiflora Rmu_sc0003074.1_g000005
rosa_roxburghii Rroxscaffold_6G00415610
rosa_rugosa Rorug03G0072700
rosa_samantha Rh3BG134900 Rh3CG137400 Rh3DG136200
rosa_wichuraiana Rw3G011030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 923, 953
AccBSI CCGCTC 1 cut(s) 629
AccII CGCG 2 cut(s) 423, 677
AccIII TCCGGA 1 cut(s) 863
AclWI GGATC 3 cut(s) 468, 521, 534
AcoI YGGCCR 3 cut(s) 450, 812, 1169
AcuI CTGAAG 2 cut(s) 1046, 1094
AcyI GRCGYC 1 cut(s) 930
AfaI GTAC 1 cut(s) 848
AfiI CCNNNNNNNGG 3 cut(s) 170, 268, 1212
AgsI TTSAA 2 cut(s) 298, 304
AjnI CCWGG 3 cut(s) 455, 522, 1206
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 8 cut(s) 79, 476, 640, 830, 875, 1007, 1060, 1114
AluI AGCT 8 cut(s) 79, 476, 640, 830, 875, 1007, 1060, 1114
Alw21I GWGCWC 2 cut(s) 81, 694
Alw26I GTCTC 2 cut(s) 215, 556
AlwI GGATC 3 cut(s) 468, 521, 534
AlwNI CAGNNNCTG 2 cut(s) 578, 1024
Ama87I CYCGRG 2 cut(s) 415, 1008
Aor13HI TCCGGA 1 cut(s) 863
AoxI GGCC 5 cut(s) 450, 517, 785, 812, 1169
ApeKI GCWGC 9 cut(s) 466, 473, 476, 578, 649, 835, 838, 1150, 1188
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
Asp700I GAANNNNTTC 1 cut(s) 541
AspLEI GCGC 2 cut(s) 677, 1161
AspS9I GGNCC 1 cut(s) 861
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 2 cut(s) 415, 1008
AvaII GGWCC 1 cut(s) 861
BamHI GGATCC 1 cut(s) 526
BanI GGYRCC 2 cut(s) 923, 953
BanII GRGCYC 2 cut(s) 81, 416
BbsI GAAGAC 3 cut(s) 66, 246, 925
Bbv12I GWGCWC 2 cut(s) 81, 694
BbvI GCAGC 9 cut(s) 453, 463, 485, 565, 661, 847, 850, 1162, 1200
BccI CCATC 2 cut(s) 811, 907
BceAI ACGGC 2 cut(s) 136, 184
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 3 cut(s) 457, 524, 1208
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 215, 556
BfmI CTRYAG 1 cut(s) 477
BglI GCCNNNNNGGC 1 cut(s) 951
BlpI GCTNAGC 1 cut(s) 831
Bme1390I CCNGG 4 cut(s) 253, 457, 524, 1208
Bme18I GGWCC 1 cut(s) 861
BmeT110I CYCGRG 2 cut(s) 415, 1008
BmgT120I GGNCC 1 cut(s) 861
BmiI GGNNCC 6 cut(s) 35, 528, 862, 925, 955, 1202
BmrFI CCNGG 4 cut(s) 253, 457, 524, 1208
BmrI ACTGGG 1 cut(s) 433
BmsI GCATC 3 cut(s) 121, 565, 1170
BmuI ACTGGG 1 cut(s) 433
BpiI GAAGAC 3 cut(s) 66, 246, 925
BplI GAGNNNNNCTC 4 cut(s) 68, 100, 748, 780
Bpu1102I GCTNAGC 1 cut(s) 831
BpuMI CCSGG 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 930
BsaJI CCNNGG 4 cut(s) 72, 421, 456, 1206
BsaWI WCCGGW 1 cut(s) 863
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 268, 1212
Bse118I RCCGGY 3 cut(s) 205, 448, 926
Bse1I ACTGG 2 cut(s) 439, 980
BseAI TCCGGA 1 cut(s) 863
BseBI CCWGG 3 cut(s) 457, 524, 1208
BseDI CCNNGG 4 cut(s) 72, 421, 456, 1206
BseGI GGATG 1 cut(s) 1051
BseLI CCNNNNNNNGG 3 cut(s) 170, 268, 1212
BseMII CTCAG 3 cut(s) 216, 644, 734
BseNI ACTGG 2 cut(s) 439, 980
BseX3I CGGCCG 1 cut(s) 1169
BseXI GCAGC 9 cut(s) 453, 463, 485, 565, 661, 847, 850, 1162, 1200
BseYI CCCAGC 2 cut(s) 1003, 1056
BsgI GTGCAG 1 cut(s) 1037
Bsh1236I CGCG 2 cut(s) 423, 677
Bsh1285I CGRYCG 2 cut(s) 210, 1172
BshFI GGCC 5 cut(s) 452, 519, 787, 814, 1171
BshNI GGYRCC 2 cut(s) 923, 953
BsiEI CGRYCG 2 cut(s) 210, 1172
BsiHKAI GWGCWC 2 cut(s) 81, 694
BsiHKCI CYCGRG 2 cut(s) 415, 1008
BsiSI CCGG 6 cut(s) 206, 252, 449, 864, 921, 927
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 3 cut(s) 170, 268, 1212
BsmAI GTCTC 2 cut(s) 215, 556
BsmBI CGTCTC 1 cut(s) 556
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 5 cut(s) 452, 519, 787, 814, 1171
BsoBI CYCGRG 2 cut(s) 415, 1008
Bsp1286I GDGCHC 3 cut(s) 81, 416, 694
Bsp13I TCCGGA 1 cut(s) 863
Bsp143I GATC 2 cut(s) 460, 526
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 1 cut(s) 72
BspANI GGCC 5 cut(s) 452, 519, 787, 814, 1171
BspCNI CTCAG 3 cut(s) 217, 643, 733
BspEI TCCGGA 1 cut(s) 863
BspFNI CGCG 2 cut(s) 423, 677
BspLI GGNNCC 6 cut(s) 35, 528, 862, 925, 955, 1202
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 3 cut(s) 468, 521, 534
BspQI GCTCTTC 1 cut(s) 1113
BspT107I GGYRCC 2 cut(s) 923, 953
BsrBI CCGCTC 1 cut(s) 629
BsrFI RCCGGY 3 cut(s) 205, 448, 926
BsrI ACTGG 2 cut(s) 439, 980
BssAI RCCGGY 3 cut(s) 205, 448, 926
BssECI CCNNGG 4 cut(s) 72, 421, 456, 1206
BssMI GATC 2 cut(s) 460, 526
BssNI GRCGYC 1 cut(s) 930
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 3 cut(s) 457, 524, 1208
Bst4CI ACNGT 2 cut(s) 120, 994
Bst6I CTCTTC 1 cut(s) 1113
BstACI GRCGYC 1 cut(s) 930
BstAPI GCANNNNNTGC 1 cut(s) 396
BstC8I GCNNGC 5 cut(s) 450, 471, 624, 1020, 1176
BstDEI CTNAG 4 cut(s) 225, 630, 720, 831
BstDSI CCRYGG 2 cut(s) 72, 421
BstF5I GGATG 1 cut(s) 1051
BstFNI CGCG 2 cut(s) 423, 677
BstHHI GCGC 2 cut(s) 677, 1161
BstKTI GATC 2 cut(s) 463, 529
BstMAI GTCTC 2 cut(s) 215, 556
BstMBI GATC 2 cut(s) 460, 526
BstMCI CGRYCG 2 cut(s) 210, 1172
BstNI CCWGG 3 cut(s) 457, 524, 1208
BstNSI RCATGY 2 cut(s) 473, 1048
BstSCI CCNGG 4 cut(s) 251, 455, 522, 1206
BstSFI CTRYAG 1 cut(s) 477
BstUI CGCG 2 cut(s) 423, 677
BstV1I GCAGC 9 cut(s) 453, 463, 485, 565, 661, 847, 850, 1162, 1200
BstV2I GAAGAC 3 cut(s) 66, 246, 925
BstX2I RGATCY 2 cut(s) 460, 526
BstXI CCANNNNNNTGG 2 cut(s) 345, 810
BstYI RGATCY 2 cut(s) 460, 526
BstZI CGGCCG 1 cut(s) 1169
BsuRI GGCC 5 cut(s) 452, 519, 787, 814, 1171
BtgI CCRYGG 2 cut(s) 72, 421
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 1051
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 397
Cac8I GCNNGC 5 cut(s) 450, 471, 624, 1020, 1176
CaiI CAGNNNCTG 2 cut(s) 578, 1024
CfoI GCGC 2 cut(s) 677, 1161
Cfr10I RCCGGY 3 cut(s) 205, 448, 926
Cfr13I GGNCC 1 cut(s) 861
Cfr42I CCGCGG 1 cut(s) 424
CseI GACGC 3 cut(s) 134, 919, 1055
Csp6I GTAC 1 cut(s) 847
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviAII CATG 5 cut(s) 73, 470, 502, 810, 1045
CviQI GTAC 1 cut(s) 847
DdeI CTNAG 4 cut(s) 225, 630, 720, 831
DpnI GATC 2 cut(s) 462, 528
DpnII GATC 2 cut(s) 460, 526
EaeI YGGCCR 3 cut(s) 450, 812, 1169
EagI CGGCCG 1 cut(s) 1169
Eam1104I CTCTTC 1 cut(s) 1113
EarI CTCTTC 1 cut(s) 1113
EciI GGCGGA 1 cut(s) 699
Ecl136II GAGCTC 1 cut(s) 79
EclXI CGGCCG 1 cut(s) 1169
Eco130I CCWWGG 1 cut(s) 72
Eco147I AGGCCT 1 cut(s) 519
Eco24I GRGCYC 2 cut(s) 81, 416
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 861
Eco52I CGGCCG 1 cut(s) 1169
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 1046, 1094
Eco88I CYCGRG 2 cut(s) 415, 1008
EcoICRI GAGCTC 1 cut(s) 79
EcoRII CCWGG 3 cut(s) 455, 522, 1206
EcoRV GATATC 1 cut(s) 704
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 2 cut(s) 81, 416
ErhI CCWWGG 1 cut(s) 72
Esp3I CGTCTC 1 cut(s) 556
FaeI CATG 5 cut(s) 76, 473, 505, 813, 1048
FaiI YATR 5 cut(s) 74, 471, 503, 811, 1046
FaqI GGGAC 1 cut(s) 542
FatI CATG 5 cut(s) 72, 469, 501, 809, 1044
FauI CCCGC 4 cut(s) 76, 269, 950, 977
FokI GGATG 1 cut(s) 1038
FriOI GRGCYC 2 cut(s) 81, 416
GlaI GCGC 2 cut(s) 676, 1160
GsaI CCCAGC 2 cut(s) 1007, 1060
HaeIII GGCC 5 cut(s) 452, 519, 787, 814, 1171
HapII CCGG 6 cut(s) 206, 252, 449, 864, 921, 927
HgaI GACGC 3 cut(s) 134, 919, 1055
HhaI GCGC 2 cut(s) 677, 1161
Hin1I GRCGYC 1 cut(s) 930
Hin1II CATG 5 cut(s) 76, 473, 505, 813, 1048
Hin6I GCGC 2 cut(s) 675, 1159
HinP1I GCGC 2 cut(s) 675, 1159
HinfI GANTC 6 cut(s) 46, 212, 373, 718, 959, 1011
HpaII CCGG 6 cut(s) 206, 252, 449, 864, 921, 927
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 1215
Hpy188I TCNGA 9 cut(s) 113, 138, 193, 331, 887, 964, 1026, 1093, 1119
Hpy188III TCNNGA 2 cut(s) 284, 864
Hpy8I GTNNAC 1 cut(s) 1215
Hpy99I CGWCG 6 cut(s) 59, 128, 193, 290, 293, 1071
HpyAV CCTTC 3 cut(s) 133, 552, 658
HpyCH4III ACNGT 2 cut(s) 120, 994
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 7 cut(s) 469, 473, 479, 809, 823, 1018, 1048
HpyF3I CTNAG 4 cut(s) 225, 630, 720, 831
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 1 cut(s) 930
Hsp92II CATG 5 cut(s) 76, 473, 505, 813, 1048
HspAI GCGC 2 cut(s) 675, 1159
Kpn2I TCCGGA 1 cut(s) 863
KroI GCCGGC 1 cut(s) 448
KroNI GCCGGC 1 cut(s) 450
KspI CCGCGG 1 cut(s) 424
Kzo9I GATC 2 cut(s) 460, 526
LguI GCTCTTC 1 cut(s) 1113
LmnI GCTCC 8 cut(s) 39, 76, 139, 667, 689, 992, 1119, 1206
Lsp1109I GCAGC 9 cut(s) 453, 463, 485, 565, 661, 847, 850, 1162, 1200
LweI GCATC 3 cut(s) 121, 565, 1170
MaeII ACGT 1 cut(s) 11
MalI GATC 2 cut(s) 462, 528
MbiI CCGCTC 1 cut(s) 629
MboI GATC 2 cut(s) 460, 526
MboII GAAGA 4 cut(s) 71, 251, 925, 1100
MflI RGATCY 2 cut(s) 460, 526
MhlI GDGCHC 3 cut(s) 81, 416, 694
MluCI AATT 1 cut(s) 909
MlyI GAGTC 4 cut(s) 221, 367, 712, 1020
MmeI TCCRAC 3 cut(s) 111, 171, 336
MroI TCCGGA 1 cut(s) 863
MroNI GCCGGC 1 cut(s) 448
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 2 cut(s) 636, 1074
MslI CAYNNNNRTG 1 cut(s) 808
MspA1I CMGCKG 2 cut(s) 423, 476
MspI CCGG 6 cut(s) 206, 252, 449, 864, 921, 927
MspR9I CCNGG 4 cut(s) 253, 457, 524, 1208
MvaI CCWGG 3 cut(s) 457, 524, 1208
MvnI CGCG 2 cut(s) 423, 677
NaeI GCCGGC 1 cut(s) 450
NciI CCSGG 1 cut(s) 253
NcoI CCATGG 1 cut(s) 72
NdeII GATC 2 cut(s) 460, 526
NgoMIV GCCGGC 1 cut(s) 448
NlaIII CATG 5 cut(s) 76, 473, 505, 813, 1048
NlaIV GGNNCC 6 cut(s) 35, 528, 862, 925, 955, 1202
NmeAIII GCCGAG 1 cut(s) 763
NspI RCATGY 2 cut(s) 473, 1048
PaeI GCATGC 1 cut(s) 473
PaeR7I CTCGAG 2 cut(s) 415, 1008
PceI AGGCCT 1 cut(s) 519
PciSI GCTCTTC 1 cut(s) 1113
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdiI GCCGGC 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 46, 959
PfoI TCCNGGA 1 cut(s) 522
PleI GAGTC 4 cut(s) 220, 367, 712, 1019
PpsI GAGTC 4 cut(s) 220, 367, 712, 1019
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 3 cut(s) 455, 522, 1206
PspFI CCCAGC 2 cut(s) 1003, 1056
PspGI CCWGG 3 cut(s) 455, 522, 1206
PspN4I GGNNCC 6 cut(s) 35, 528, 862, 925, 955, 1202
PspPI GGNCC 1 cut(s) 861
PspXI VCTCGAGB 2 cut(s) 415, 1008
PsrI GAACNNNNNNTAC 2 cut(s) 1079, 1111
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 2 cut(s) 578, 1024
PsuI RGATCY 2 cut(s) 460, 526
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 1 cut(s) 848
RsaNI GTAC 1 cut(s) 847
RseI CAYNNNNRTG 1 cut(s) 808
SacI GAGCTC 1 cut(s) 81
SacII CCGCGG 1 cut(s) 424
SapI GCTCTTC 1 cut(s) 1113
SaqAI TTAA 2 cut(s) 636, 1074
Sau3AI GATC 2 cut(s) 460, 526
Sau96I GGNCC 1 cut(s) 861
SchI GAGTC 4 cut(s) 221, 367, 712, 1020
ScrFI CCNGG 4 cut(s) 253, 457, 524, 1208
SduI GDGCHC 3 cut(s) 81, 416, 694
SfaNI GCATC 3 cut(s) 121, 565, 1170
SfcI CTRYAG 1 cut(s) 477
Sfr274I CTCGAG 2 cut(s) 415, 1008
Sfr303I CCGCGG 1 cut(s) 424
SgrAI CRCCGGYG 1 cut(s) 926
SgrBI CCGCGG 1 cut(s) 424
SinI GGWCC 1 cut(s) 861
SlaI CTCGAG 2 cut(s) 415, 1008
SmiMI CAYNNNNRTG 1 cut(s) 808
SmlI CTYRAG 2 cut(s) 415, 1008
SmoI CTYRAG 2 cut(s) 415, 1008
SphI GCATGC 1 cut(s) 473
Sse9I AATT 1 cut(s) 909
SseBI AGGCCT 1 cut(s) 519
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 519
StyD4I CCNGG 4 cut(s) 251, 455, 522, 1206
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 120, 994
TaiI ACGT 1 cut(s) 14
TaqI TCGA 8 cut(s) 123, 210, 215, 285, 371, 416, 700, 1009
TasI AATT 1 cut(s) 909
TatI WGTACW 1 cut(s) 846
TfiI GAWTC 2 cut(s) 46, 959
Tru1I TTAA 2 cut(s) 636, 1074
Tru9I TTAA 2 cut(s) 636, 1074
TscAI CASTG 1 cut(s) 397
TseI GCWGC 9 cut(s) 466, 473, 476, 578, 649, 835, 838, 1150, 1188
TspDTI ATGAA 2 cut(s) 518, 677
TspRI CASTG 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 861
XceI RCATGY 2 cut(s) 473, 1048
XhoI CTCGAG 2 cut(s) 415, 1008
XmnI GAANNNNTTC 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.