MD04G1140200.v1.1

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011807
Physical Location & Seq
Forward (+)
22850907 .. 22853899
2993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1140200.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGACGATACGTTAGACGCTGCGTTTTGGCTCCCGACCCAGATTCTCAACGACGACGACGCACCGGCCATGGAGCTCAACTCAAAGACGAAGAAAAGTGGCGGGTATGGGTTTGACACTGACGCTCCGAAAGCTCTGTTCCCGTTTGAGTTTCCTTACGGCGGCGCTTTTGGGGTTTCGTCGGATTTTAGTTCGCCGGTCGAGTCGAGCGAGACTGAGAGCGACGAGGAAGACTACCTCGTTGGGTTGACCCGCCAAATGGCTCGCTCCACTCTCGACGACGAGTTCAAATACTCCGACTCTGCTTTCGCCTTCGAGAAGCCAAAGGGTTGGGTCGCTTCTGGTTCTCCCCAATCGACTCTCTGCGCAGTCGGGAGCGGTTGCGGTTGCGGACAGGGCTCGAGCCGTGGAAGCCCTAACGCCCAGTCGCCGCCGGCCACCTGGGATCTGCTGCATGCGGCTGCAGGGGAAGTAGCCAAGATGCGCATCAACCAAAGCAGAGGCCTCCTGGGTCCTCCAAGAAAGCCTTCCCCTGTCTCGGTCCCCACCAACAACCACCCAGATGCTGCTTATTACTACCACCAGCAATCTCTTTCTCACAAGCACTTGCGAGCCGCTCAGTTTGAGCTGTTGAGGCAGCAACAGATGATGAAGGAGCAGAACGCGGCGGTCTGGGGAGCACAAACTCGATATCCTCCGGCGCAGACTCAGCTGCAGCATCACCTAAACCACCAAACAAGAGCGAGAAACAACACTCCCAACATTCTCAATGCTCGGCCTCTGGGTTTGTCGCCATCTGCATGGCCGCCTCTGCAACAAGCTCAGCGGCAGCAACAGTACAACAAAAACGGTTTCGGATTCAGAGCGGTTTATGTTGGAAACCAAAACCCATCTGCGAAAGTTGACCGTTCCGGCACCGGCGTCTTCTTACCCCGCCAAGTCGGCCCCCAATTCGAACCCCGCAAGAAGCAAGTTTGTTCCACAGCTCTACTCCCAGCTAGAGTTGTGCAGGCTCTGAACTTGAACGTTGACGACATGCAGCAACAGCCCCAGCTTCAGCGTCGTTTTAATGGCAGACTGAACTCTGATTACGAGGTTGCTCTTCAGCTTCGGAGTAAGGCCGCCGCCACCAGAACCGCCACTTTGCAGCAGAAGCGCAATATTTGGCCGCAGCCGGCAGCGAGCAACGAGATTAGGCTCCCCCAGGAGTGGACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

44.94

Weight (kDa)

9.04

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54000 AT3G54000 AT3G54000
fragaria_vesca FvH4_6g12650
malus_domestica MD04G1140200.v1.1 MD12G1155400.v1.1 MD15G1149300.v1.1
prunus_persica Prupe.6G265300_v2.0.a1
pyrus_communis pycom04g12780 pycom12g14750
rosa_chinensis RchiOBHm_Chr3g0464501
rosa_laevigata RLG00000024691
rosa_multiflora Rmu_sc0003074.1_g000005
rosa_roxburghii Rroxscaffold_6G00415610
rosa_rugosa Rorug03G0072700
rosa_samantha Rh3BG134900 Rh3CG137400 Rh3DG136200
rosa_wichuraiana Rw3G011030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 367, 485
AccB1I GGYRCC 1 cut(s) 914
AccBSI CCGCTC 3 cut(s) 378, 617, 866
AccII CGCG 1 cut(s) 665
AclI AACGTT 1 cut(s) 1026
AclWI GGATC 1 cut(s) 453
AcoI YGGCCR 4 cut(s) 66, 435, 803, 1166
AcuI CTGAAG 2 cut(s) 1040, 1088
AcyI GRCGYC 1 cut(s) 921
AfaI GTAC 1 cut(s) 839
AfiI CCNNNNNNNGG 4 cut(s) 161, 259, 538, 1209
AgsI TTSAA 2 cut(s) 289, 1024
AjnI CCWGG 3 cut(s) 440, 507, 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AluBI AGCT 9 cut(s) 76, 134, 628, 712, 821, 986, 998, 1054, 1108
AluI AGCT 9 cut(s) 76, 134, 628, 712, 821, 986, 998, 1054, 1108
Alw21I GWGCWC 2 cut(s) 78, 682
Alw26I GTCTC 2 cut(s) 206, 541
AlwI GGATC 1 cut(s) 453
AlwNI CAGNNNCTG 2 cut(s) 566, 1015
Ama87I CYCGRG 1 cut(s) 400
AoxI GGCC 8 cut(s) 66, 435, 502, 776, 803, 943, 1119, 1166
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
Asp700I GAANNNNTTC 1 cut(s) 526
AspLEI GCGC 5 cut(s) 167, 368, 486, 703, 1158
AspS9I GGNCC 3 cut(s) 512, 541, 944
AsuHPI GGTGA 1 cut(s) 713
AsuII TTCGAA 1 cut(s) 954
AvaI CYCGRG 1 cut(s) 400
AvaII GGWCC 2 cut(s) 512, 541
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 2 cut(s) 78, 401
BbsI GAAGAC 2 cut(s) 237, 916
Bbv12I GWGCWC 2 cut(s) 78, 682
BccI CCATC 2 cut(s) 802, 898
BceAI ACGGC 2 cut(s) 175, 390
BciT130I CCWGG 3 cut(s) 442, 509, 1205
BcoDI GTCTC 2 cut(s) 206, 541
BfaI CTAG 2 cut(s) 999, 1219
BfmI CTRYAG 2 cut(s) 462, 713
BfoI RGCGCY 1 cut(s) 168
BglI GCCNNNNNGGC 1 cut(s) 942
BlpI GCTNAGC 1 cut(s) 822
Bme1390I CCNGG 3 cut(s) 442, 509, 1205
Bme18I GGWCC 2 cut(s) 512, 541
BmeT110I CYCGRG 1 cut(s) 400
BmgT120I GGNCC 3 cut(s) 512, 541, 944
BmiI GGNNCC 6 cut(s) 32, 513, 543, 916, 946, 1199
BmrFI CCNGG 3 cut(s) 442, 509, 1205
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 4 cut(s) 471, 495, 553, 727
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 2 cut(s) 237, 916
BplI GAGNNNNNCTC 2 cut(s) 65, 97
Bpu1102I GCTNAGC 1 cut(s) 822
Bpu14I TTCGAA 1 cut(s) 954
BsaBI GATNNNNATC 1 cut(s) 485
BsaHI GRCGYC 1 cut(s) 921
BsaJI CCNNGG 5 cut(s) 69, 406, 441, 508, 1203
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 259, 538, 1209
Bse118I RCCGGY 5 cut(s) 64, 196, 433, 917, 1174
Bse1I ACTGG 1 cut(s) 424
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 3 cut(s) 442, 509, 1205
BseDI CCNNGG 5 cut(s) 69, 406, 441, 508, 1203
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 4 cut(s) 161, 259, 538, 1209
BseMII CTCAG 4 cut(s) 207, 632, 722, 836
BseNI ACTGG 1 cut(s) 424
BseYI CCCAGC 2 cut(s) 994, 1050
BsgI GTGCAG 1 cut(s) 1028
Bsh1236I CGCG 1 cut(s) 665
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 8 cut(s) 68, 437, 504, 778, 805, 945, 1121, 1168
BshNI GGYRCC 1 cut(s) 914
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 78, 682
BsiHKCI CYCGRG 1 cut(s) 400
BsiSI CCGG 7 cut(s) 65, 197, 434, 698, 912, 918, 1175
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 4 cut(s) 161, 259, 538, 1209
BsmAI GTCTC 2 cut(s) 206, 541
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 8 cut(s) 68, 437, 504, 778, 805, 945, 1121, 1168
BsoBI CYCGRG 1 cut(s) 400
Bsp119I TTCGAA 1 cut(s) 954
Bsp1286I GDGCHC 3 cut(s) 78, 401, 682
Bsp143I GATC 1 cut(s) 445
Bsp1720I GCTNAGC 1 cut(s) 822
Bsp19I CCATGG 1 cut(s) 69
BspANI GGCC 8 cut(s) 68, 437, 504, 778, 805, 945, 1121, 1168
BspCNI CTCAG 4 cut(s) 208, 631, 721, 835
BspFNI CGCG 1 cut(s) 665
BspLI GGNNCC 6 cut(s) 32, 513, 543, 916, 946, 1199
BspMAI CTGCAG 2 cut(s) 466, 717
BspPI GGATC 1 cut(s) 453
BspQI GCTCTTC 1 cut(s) 1107
BspT104I TTCGAA 1 cut(s) 954
BspT107I GGYRCC 1 cut(s) 914
BsrBI CCGCTC 3 cut(s) 378, 617, 866
BsrFI RCCGGY 5 cut(s) 64, 196, 433, 917, 1174
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 5 cut(s) 64, 196, 433, 917, 1174
BssECI CCNNGG 5 cut(s) 69, 406, 441, 508, 1203
BssMI GATC 1 cut(s) 445
BssNI GRCGYC 1 cut(s) 921
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 3 cut(s) 442, 509, 1205
Bst4CI ACNGT 3 cut(s) 837, 851, 908
Bst6I CTCTTC 1 cut(s) 1107
BstACI GRCGYC 1 cut(s) 921
BstBI TTCGAA 1 cut(s) 954
BstC8I GCNNGC 7 cut(s) 265, 435, 456, 612, 1011, 1176, 1183
BstDEI CTNAG 4 cut(s) 216, 618, 708, 822
BstDSI CCRYGG 2 cut(s) 69, 406
BstFNI CGCG 1 cut(s) 665
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 5 cut(s) 167, 368, 486, 703, 1158
BstKTI GATC 1 cut(s) 448
BstMAI GTCTC 2 cut(s) 206, 541
BstMBI GATC 1 cut(s) 445
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 9 cut(s) 131, 396, 411, 634, 709, 811, 942, 1045, 1153
BstNI CCWGG 3 cut(s) 442, 509, 1205
BstNSI RCATGY 2 cut(s) 458, 1039
BstSCI CCNGG 3 cut(s) 440, 507, 1203
BstSFI CTRYAG 2 cut(s) 462, 713
BstUI CGCG 1 cut(s) 665
BstV2I GAAGAC 2 cut(s) 237, 916
BstX2I RGATCY 1 cut(s) 445
BstXI CCANNNNNNTGG 2 cut(s) 330, 801
BstYI RGATCY 1 cut(s) 445
BsuRI GGCC 8 cut(s) 68, 437, 504, 778, 805, 945, 1121, 1168
BtgI CCRYGG 2 cut(s) 69, 406
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 7 cut(s) 265, 435, 456, 612, 1011, 1176, 1183
CaiI CAGNNNCTG 2 cut(s) 566, 1015
CfoI GCGC 5 cut(s) 167, 368, 486, 703, 1158
Cfr10I RCCGGY 5 cut(s) 64, 196, 433, 917, 1174
Cfr13I GGNCC 3 cut(s) 512, 541, 944
CseI GACGC 5 cut(s) 26, 68, 131, 910, 1049
Csp6I GTAC 1 cut(s) 838
CviAII CATG 4 cut(s) 70, 455, 801, 1036
CviQI GTAC 1 cut(s) 838
DdeI CTNAG 4 cut(s) 216, 618, 708, 822
DpnI GATC 1 cut(s) 447
DpnII GATC 1 cut(s) 445
EaeI YGGCCR 4 cut(s) 66, 435, 803, 1166
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 69
Eco147I AGGCCT 1 cut(s) 504
Eco24I GRGCYC 2 cut(s) 78, 401
Eco32I GATATC 1 cut(s) 692
Eco47I GGWCC 2 cut(s) 512, 541
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 2 cut(s) 1040, 1088
Eco88I CYCGRG 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 512
EcoRII CCWGG 3 cut(s) 440, 507, 1203
EcoRV GATATC 1 cut(s) 692
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 2 cut(s) 78, 401
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 4 cut(s) 73, 458, 804, 1039
FaiI YATR 6 cut(s) 71, 108, 456, 802, 873, 1037
FalI AAGNNNNNCTT 2 cut(s) 511, 543
FaqI GGGAC 1 cut(s) 527
FatI CATG 4 cut(s) 69, 454, 800, 1035
FauI CCCGC 4 cut(s) 95, 260, 941, 968
FriOI GRGCYC 2 cut(s) 78, 401
FspAI RTGCGCAY 1 cut(s) 485
FspBI CTAG 2 cut(s) 999, 1219
FspI TGCGCA 2 cut(s) 367, 485
GlaI GCGC 5 cut(s) 166, 367, 485, 702, 1157
GsaI CCCAGC 2 cut(s) 998, 1054
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 8 cut(s) 68, 437, 504, 778, 805, 945, 1121, 1168
HapII CCGG 7 cut(s) 65, 197, 434, 698, 912, 918, 1175
HgaI GACGC 5 cut(s) 26, 68, 131, 910, 1049
HhaI GCGC 5 cut(s) 167, 368, 486, 703, 1158
Hin1I GRCGYC 1 cut(s) 921
Hin1II CATG 4 cut(s) 73, 458, 804, 1039
Hin6I GCGC 5 cut(s) 165, 366, 484, 701, 1156
HinP1I GCGC 5 cut(s) 165, 366, 484, 701, 1156
HincII GTYRAC 3 cut(s) 249, 904, 1030
HindII GTYRAC 3 cut(s) 249, 904, 1030
HinfI GANTC 6 cut(s) 43, 203, 299, 358, 706, 858
HpaII CCGG 7 cut(s) 65, 197, 434, 698, 912, 918, 1175
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 4 cut(s) 249, 904, 1030, 1212
Hpy188I TCNGA 8 cut(s) 129, 184, 298, 857, 863, 1017, 1087, 1113
Hpy188III TCNNGA 4 cut(s) 34, 275, 316, 373
Hpy8I GTNNAC 4 cut(s) 249, 904, 1030, 1212
Hpy99I CGWCG 8 cut(s) 56, 59, 62, 184, 227, 281, 284, 1065
HpyAV CCTTC 3 cut(s) 322, 537, 646
HpyCH4III ACNGT 3 cut(s) 837, 851, 908
HpyCH4IV ACGT 2 cut(s) 11, 1026
HpyCH4V TGCA 8 cut(s) 454, 464, 715, 800, 814, 1009, 1039, 1147
HpyF10VI GCNNNNNNNGC 9 cut(s) 131, 396, 411, 634, 709, 811, 942, 1045, 1153
HpyF3I CTNAG 4 cut(s) 216, 618, 708, 822
HpySE526I ACGT 2 cut(s) 11, 1026
Hsp92I GRCGYC 1 cut(s) 921
Hsp92II CATG 4 cut(s) 73, 458, 804, 1039
HspAI GCGC 5 cut(s) 165, 366, 484, 701, 1156
KroI GCCGGC 2 cut(s) 433, 1174
KroNI GCCGGC 2 cut(s) 435, 1176
Kzo9I GATC 1 cut(s) 445
LguI GCTCTTC 1 cut(s) 1107
LmnI GCTCC 8 cut(s) 36, 73, 130, 272, 375, 655, 677, 1203
LweI GCATC 4 cut(s) 471, 495, 553, 727
MaeI CTAG 2 cut(s) 999, 1219
MaeII ACGT 2 cut(s) 11, 1026
MalI GATC 1 cut(s) 447
MbiI CCGCTC 3 cut(s) 378, 617, 866
MboI GATC 1 cut(s) 445
MboII GAAGA 4 cut(s) 103, 242, 916, 1094
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 3 cut(s) 78, 401, 682
MluCI AATT 1 cut(s) 950
MlyI GAGTC 4 cut(s) 212, 293, 352, 700
MmeI TCCRAC 3 cut(s) 162, 321, 856
MroNI GCCGGC 2 cut(s) 433, 1174
MroXI GAANNNNTTC 1 cut(s) 526
MseI TTAA 1 cut(s) 1068
MslI CAYNNNNRTG 2 cut(s) 561, 799
MspA1I CMGCKG 2 cut(s) 712, 826
MspI CCGG 7 cut(s) 65, 197, 434, 698, 912, 918, 1175
MspR9I CCNGG 3 cut(s) 442, 509, 1205
MvaI CCWGG 3 cut(s) 442, 509, 1205
MvnI CGCG 1 cut(s) 665
MwoI GCNNNNNNNGC 9 cut(s) 131, 396, 411, 634, 709, 811, 942, 1045, 1153
NaeI GCCGGC 2 cut(s) 435, 1176
NcoI CCATGG 1 cut(s) 69
NdeII GATC 1 cut(s) 445
NgoMIV GCCGGC 2 cut(s) 433, 1174
NlaIII CATG 4 cut(s) 73, 458, 804, 1039
NlaIV GGNNCC 6 cut(s) 32, 513, 543, 916, 946, 1199
NmeAIII GCCGAG 1 cut(s) 754
NsbI TGCGCA 2 cut(s) 367, 485
NspI RCATGY 2 cut(s) 458, 1039
NspV TTCGAA 1 cut(s) 954
PaeI GCATGC 1 cut(s) 458
PaeR7I CTCGAG 1 cut(s) 400
PceI AGGCCT 1 cut(s) 504
PciSI GCTCTTC 1 cut(s) 1107
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdiI GCCGGC 2 cut(s) 435, 1176
PdmI GAANNNNTTC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 43, 858
PleI GAGTC 4 cut(s) 211, 293, 352, 700
PpsI GAGTC 4 cut(s) 211, 293, 352, 700
PpuMI RGGWCCY 1 cut(s) 512
Psp124BI GAGCTC 1 cut(s) 78
Psp1406I AACGTT 1 cut(s) 1026
Psp5II RGGWCCY 1 cut(s) 512
Psp6I CCWGG 3 cut(s) 440, 507, 1203
PspFI CCCAGC 2 cut(s) 994, 1050
PspGI CCWGG 3 cut(s) 440, 507, 1203
PspN4I GGNNCC 6 cut(s) 32, 513, 543, 916, 946, 1199
PspPI GGNCC 3 cut(s) 512, 541, 944
PspPPI RGGWCCY 1 cut(s) 512
PspXI VCTCGAGB 1 cut(s) 400
PsrI GAACNNNNNNTAC 2 cut(s) 1073, 1105
PstI CTGCAG 2 cut(s) 466, 717
PstNI CAGNNNCTG 2 cut(s) 566, 1015
PsuI RGATCY 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 712
RsaI GTAC 1 cut(s) 839
RsaNI GTAC 1 cut(s) 838
RseI CAYNNNNRTG 2 cut(s) 561, 799
SacI GAGCTC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 1107
SaqAI TTAA 1 cut(s) 1068
Sau3AI GATC 1 cut(s) 445
Sau96I GGNCC 3 cut(s) 512, 541, 944
SchI GAGTC 4 cut(s) 212, 293, 352, 700
ScrFI CCNGG 3 cut(s) 442, 509, 1205
SduI GDGCHC 3 cut(s) 78, 401, 682
SfaNI GCATC 4 cut(s) 471, 495, 553, 727
SfcI CTRYAG 2 cut(s) 462, 713
Sfr274I CTCGAG 1 cut(s) 400
SfuI TTCGAA 1 cut(s) 954
SgrAI CRCCGGYG 1 cut(s) 917
SinI GGWCC 2 cut(s) 512, 541
SlaI CTCGAG 1 cut(s) 400
SmiMI CAYNNNNRTG 2 cut(s) 561, 799
SmlI CTYRAG 1 cut(s) 400
SmoI CTYRAG 1 cut(s) 400
SphI GCATGC 1 cut(s) 458
Sse9I AATT 1 cut(s) 950
SseBI AGGCCT 1 cut(s) 504
SspI AATATT 1 cut(s) 1162
SspMI CTAG 2 cut(s) 999, 1219
SstI GAGCTC 1 cut(s) 78
StuI AGGCCT 1 cut(s) 504
StyD4I CCNGG 3 cut(s) 440, 507, 1203
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 3 cut(s) 837, 851, 908
TaiI ACGT 2 cut(s) 14, 1029
TaqI TCGA 8 cut(s) 201, 206, 276, 315, 356, 401, 688, 954
TaqII GACCGA 1 cut(s) 529
TasI AATT 1 cut(s) 950
TatI WGTACW 1 cut(s) 837
TfiI GAWTC 2 cut(s) 43, 858
Tru1I TTAA 1 cut(s) 1068
Tru9I TTAA 1 cut(s) 1068
TscAI CASTG 1 cut(s) 124
TspDTI ATGAA 1 cut(s) 665
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 512, 541
XceI RCATGY 2 cut(s) 458, 1039
XhoI CTCGAG 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 2 cut(s) 999, 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.