AT3G54440

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010440
Physical Location & Seq
Reverse (-)
20148208 .. 20157199
8992 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54440.2

Sequence Viewer

Length: 3324 bp
ATGGTTTCGTTAGCGACCCAAATGATTCTTCCATCTGAAAATGGGTATAGAGTTTGGGAGGATCAGACCCTTTTCAAATGGCGTAAGAGAGATCCTCATGTCACCTTGCGTTGCCATGAATCCGTCCAAGGGGCTTTGAGGTACTGGTATCAGCGGAACAACGTGGATCTCACCGTATCAAAATCTGCTGTTTGGAATGATGATGCTGTTCAAGCTGCTCTTGATAGCGCTGCTTTTTGGGTTGACGGCTTGCCCTTTGTTAAGTCTTTATCTGGTTACTGGAAGTTTTTCTTGGCTCCTAAACCTGCTAATGTTCCTGACAAATTTTATGATGCTGCCTTTTCTGATTCAGACTGGAATGCTTTACAGGTTCCTTCCAACTGGCAGTGTCATGGATTTGATCGGCCTATCTATACGAATGTGGTGTACCCTTTCCCGAATGATCCTCCTTATGTTCCTGAGGATAATCCTACTGGTTGCTACAGAACCTACTTCCAGATTCCCAAAGAATGGAAGGACAGAAGAATACTTCTTCACTTTGAAGCTGTAGACTCCGCATTCTTTGCTTGGATAAATGGCAACCCTGTTGGGTACAGTCAAGACAGCAGGTTACCTGCTGAATTTGAAATATCTGATTATTGCTATCCATGGGACTCGGGAAAACAAAATGTTCTTGCTGTTCAAGTCTTTAGATGGAGTGATGGTTCATACCTTGAAGACCAAGATCATTGGTGGTTGTCTGGTATTCATCGAGATGTGCTTTTGTTAGCAAAGCCCAAGGTCTTCATTGCTGACTACTTTTTCAAGTCAAAATTGGCAGATGACTTTTCATACGCAGACATCCAGGTTGAAGTGAAGATTGACAATATGCAGGAGTCCTCAAAGGATCTTGTTCTTTCTAACTTCATCATAGAGGCTGCCATATTTGATACAAAAAATTGGTACAACTCTGAAGGATTTAGTTGTGAACTTTCTCCCAAGGTGGCTAATTTGAAGCTTAATCCTTCTCCAAGCCCAACTCTTGGATTTCATGGTTACTTGCTTGAGGGAAAACTGGATTCGCCAAATCTCTGGTCAGCTGAACAACCAAATGTTTACATCCTCGTTTTAACTCTAAAAGATACATCTGGGAAAGTCCTTGATTCTGAATCAAGCATTGTGGGCATTCGGCAAGTATCAAAGGCCTTTAAACAGCTTCTTGTTAATGGGCACCCAGTCGTGATAAAAGGTGTAAACAGGCATGAGCACCATCCACGTGTTGGGAAGACGAATATAGAGGCATGCATGGTCAAGGATTTAATTATGATGAAAGAATATAATATCAATGCGGTGCGAAACAGTCATTATCCTCAACATCCCCGCTGGTATGAATTATGTGATTTGTTCGGCATGTACATGATCGATGAAGCCAATATAGAGACACATGGTTTTGATCTCTCTGGGCATCTGAAGCACCCTGCAAAAGAGCCAAGCTGGGCAGCTGCTATGCTGGATCGAGTAGTTGGGATGGTTGAAAGAGACAAAAACCATACATGCATAATTTCTTGGTCACTTGGAAATGAGGCTGGCTATGGGCCCAATCATTCTGCCATGGCAGGTTGGATTAGGGAAAAGGACCCCTCACGGTTGGTGCACTATGAAGGTGGTGGTTCCAGAACAAGTTCTACAGATATAGTCTGCCCTATGTACATGCGGGTTTGGGACATCATCAAGATTGCACTTGATCAAAATGAATCACGGCCGTTGATATTATGCGAGTATCAACATGCTATGGGTAACAGCAACGGGAATATAGATGAGTACTGGGAGGCAATTGACAATACCTTTGGCCTGCAAGGAGGTTTCATATGGGACTGGGTTGACCAGGGGCTATTGAAACTGGGGTCAGATGGCATTAAGCGTTGGGCATATGGAGGGGACTTTGGTGACCAGCCTAATGATTTGAACTTCTGTCTCAATGGGCTTATATGGCCTGACCGAACTCCTCATCCAGCACTCCATGAGGTTAAGCATTGTTACCAACCAATCAAGGTTTCATTGACGGATGGCATGATAAAGGTTGCAAACACTTACTTTTTCAACACAACAGAAGAATTGGAGTTTAGCTGGACAATTCATGGGGATGGTCTTGAACTTGGATCTGGGACTCTCTCTATTCCTGTGATAAAGCCACAAAATAGTTTTGAGATGGAGTGGAAGTCAGGTCCATGGTTTTCTTTCTGGAATGACTCAAATGCTGGAGAATTGTTTCTGACAATAAATGCCAAGCTTTTAAATCTTACTCGTTCGCTTGAAGCCGGTCATCTTTTGTCTTCAACGCAGATTCCTTTACCAGCAAAGGGACAAATAATACCGCAGGCGATAAAAAAGACAGACACTAGTATCACTTGTGAAACTGTTGGAGATTTCATTAAGATCAGCCAGAAAGATTCATGGGAGCTAATGGTAAATGTCCGAAAAGGGACTATTGAAGGTTGGAAGATTCAAGGAGTTCTCCTTATGAACGAAGCTATACTGCCATGCTTTTGGCGAGCACCTACAGACAATGATAAAGGTGGAGGTGACTCTAGTTATTTTTCGAGGTGGAAAGCGGCACAGTTAGATAATGTGGAGTTCCTTGTTGAAAGCTGTTCAGTAAAGAGCATCACTGATAAATCCGTAGAGATAGAGTTCATTTACCTTGGTTCTTCAGCTTCTGGCTCTTCAAAGTCAGATGCCTTATTCAAAGTCAATGTGACATATCTGATCTATGGTTCCGGAGATATCATCACCAATTGGTTTGTAGAACCAAACTCTGATCTTCCACCGCTACCACGTGTAGGTATAGAATTCCACATTGAAAAAACACTGGACCGTGTAGAATGGTATGGCAAAGGTCCATTTGAGTGTTACCCAGACCGAAAAGCAGCAGCCCATGTGGCGATATATGAACACAATGTTGGAGACATGCATGTCCCTTATATTGTTCCAGGAGAAAATGGAGGTAGAACAGATGTTAGGTGGGTAACATTCCGAAACAAAGATGGTGTAGGAATATATGCTTCAACATATGGTAGCTCTTCTCTAATGCAAATGAATGCTAGTTATTATACAACCGGTGAGCTTCACCGTGCAACGCATGAAGAGGATCTTATCAAAGGACAAAACATCGAGGTGCATCTGGACCACAAACACATGGGTCTTGGGGGAGATGATAGCTGGACTCCATGTGTTCACGATAAGTTTCTGATTCCGCCTGCACAGTATTCGTTTTCTCTCAGGCTATGCCCAATTACTGCATCCACCTCGGGTTTGAACATCTACAAGGATCAACTCCCCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1107

Amino Acids

125.41

Weight (kDa)

5.43

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 87 - 259 1.4e-54 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 261 - 390 1.3e-14 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 397 - 677 1.1e-97 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 685 - 778 1.2e-15 Beta-galactosidase, domain 4
Bgal_small_N PF02929 809 - 1089 2.9e-72 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2951
Acc36I ACCTGC 4 cut(s) 313, 597, 622, 1586
AccB1I GGYRCC 1 cut(s) 1209
AccB7I CCANNNNNTGG 3 cut(s) 510, 1022, 1259
AccI GTMKAC 1 cut(s) 549
AccIII TCCGGA 1 cut(s) 2756
AciI CCGC 9 cut(s) 154, 555, 1328, 1360, 1693, 2354, 2591, 2807, 3233
AclWI GGATC 9 cut(s) 69, 86, 174, 437, 894, 1500, 2146, 3135, 3315
AcoI YGGCCR 1 cut(s) 1739
AcsI RAATTY 3 cut(s) 323, 620, 2828
AcuI CTGAAG 3 cut(s) 972, 1469, 2673
AcvI CACGTG 2 cut(s) 1256, 2816
AfaI GTAC 7 cut(s) 143, 428, 593, 944, 1394, 1688, 1802
AfeI AGCGCT 1 cut(s) 229
AfiI CCNNNNNNNGG 6 cut(s) 129, 510, 1022, 1259, 2339, 2819
AflIII ACRYGT 2 cut(s) 1255, 2815
AgeI ACCGGT 1 cut(s) 3095
AhlI ACTAGT 1 cut(s) 2378
AjnI CCWGG 3 cut(s) 843, 1863, 2968
AjuI GAANNNNNNNTTGG 8 cut(s) 12, 44, 275, 307, 2017, 2049, 2922, 2954
AloI GAACNNNNNNTCC 4 cut(s) 688, 720, 1972, 2004
Alw21I GWGCWC 3 cut(s) 1248, 1635, 2536
Alw26I GTCTC 4 cut(s) 1412, 1512, 1958, 2937
Alw44I GTGCAC 1 cut(s) 1631
AlwI GGATC 9 cut(s) 69, 86, 174, 437, 894, 1500, 2146, 3135, 3315
AlwNI CAGNNNCTG 1 cut(s) 2696
Ama87I CYCGRG 2 cut(s) 655, 3286
Aor13HI TCCGGA 1 cut(s) 2756
Aor51HI AGCGCT 1 cut(s) 229
AoxI GGCC 6 cut(s) 404, 1182, 1574, 1739, 1828, 1970
ApaI GGGCCC 1 cut(s) 1578
ApaLI GTGCAC 1 cut(s) 1631
ApeKI GCWGC 8 cut(s) 215, 230, 335, 917, 1478, 1481, 2906, 2909
ApoI RAATTY 3 cut(s) 323, 620, 2828
ArsI GACNNNNNNTTYG 4 cut(s) 1058, 1090, 1808, 1840
AsiGI ACCGGT 1 cut(s) 3095
Asp700I GAANNNNTTC 3 cut(s) 287, 1660, 2247
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 7 cut(s) 1574, 1575, 1615, 2204, 2851, 2876, 3163
AsuHPI GGTGA 7 cut(s) 94, 163, 1937, 2573, 2761, 3098, 3110
AvaI CYCGRG 2 cut(s) 655, 3286
AvaII GGWCC 5 cut(s) 1615, 2204, 2851, 2876, 3163
AxyI CCTNAGG 1 cut(s) 459
BaeGI GKGCMC 3 cut(s) 1212, 1578, 1635
BaeI ACNNNNGTAYC 2 cut(s) 2365, 2398
BanI GGYRCC 1 cut(s) 1209
BanII GRGCYC 1 cut(s) 1578
BbrPI CACGTG 2 cut(s) 1256, 2816
BbsI GAAGAC 4 cut(s) 723, 775, 1271, 2304
Bbv12I GWGCWC 3 cut(s) 1248, 1635, 2536
BbvI GCAGC 8 cut(s) 202, 217, 322, 904, 1468, 1490, 2918, 2921
BceAI ACGGC 3 cut(s) 262, 1726, 1754
BcgI CGANNNNNNTGC 4 cut(s) 3228, 3262, 3267, 3301
BciT130I CCWGG 3 cut(s) 845, 1865, 2970
BclI TGATCA 1 cut(s) 1723
BcoDI GTCTC 4 cut(s) 1412, 1512, 1958, 2937
BcuI ACTAGT 1 cut(s) 2378
BfaI CTAG 4 cut(s) 2379, 2568, 3081, 3322
BfmI CTRYAG 4 cut(s) 481, 546, 1665, 2538
BfoI RGCGCY 1 cut(s) 231
BfuAI ACCTGC 4 cut(s) 313, 597, 622, 1586
BglI GCCNNNNNGGC 1 cut(s) 2918
BisI GCNGC 9 cut(s) 216, 231, 336, 918, 1479, 1482, 2592, 2907, 2910
BlsI GCNGC 9 cut(s) 217, 232, 337, 919, 1480, 1483, 2593, 2908, 2911
BmcAI AGTACT 1 cut(s) 1802
Bme1390I CCNGG 3 cut(s) 845, 1865, 2970
Bme18I GGWCC 5 cut(s) 1615, 2204, 2851, 2876, 3163
BmeT110I CYCGRG 2 cut(s) 655, 3286
BmgT120I GGNCC 7 cut(s) 1574, 1575, 1615, 2204, 2851, 2876, 3163
BmiI GGNNCC 7 cut(s) 297, 372, 1211, 1576, 1617, 1651, 2755
BmrFI CCNGG 3 cut(s) 845, 1865, 2970
BmrI ACTGGG 4 cut(s) 1208, 1813, 1864, 1889
BmsI GCATC 7 cut(s) 193, 322, 1453, 2652, 2704, 3166, 3287
BmuI ACTGGG 4 cut(s) 1208, 1813, 1864, 1889
BpiI GAAGAC 4 cut(s) 723, 775, 1271, 2304
BplI GAGNNNNNCTC 2 cut(s) 79, 111
BpmI CTGGAG 1 cut(s) 2259
BpuEI CTTGAG 1 cut(s) 1064
Bsa29I ATCGAT 1 cut(s) 1401
BsaAI YACGTR 2 cut(s) 1256, 2816
BsaJI CCNNGG 9 cut(s) 127, 647, 777, 978, 1590, 1864, 2207, 2680, 3285
BsaWI WCCGGW 2 cut(s) 2756, 3095
BsaXI ACNNNNNCTCC 4 cut(s) 688, 718, 2183, 2213
Bsc4I CCNNNNNNNGG 6 cut(s) 129, 510, 1022, 1259, 2339, 2819
Bse118I RCCGGY 2 cut(s) 2297, 3095
Bse21I CCTNAGG 1 cut(s) 459
Bse3DI GCAATG 1 cut(s) 786
BseAI TCCGGA 1 cut(s) 2756
BseBI CCWGG 3 cut(s) 845, 1865, 2970
BseCI ATCGAT 1 cut(s) 1401
BseDI CCNNGG 9 cut(s) 127, 647, 777, 978, 1590, 1864, 2207, 2680, 3285
BseGI GGATG 9 cut(s) 840, 1098, 1249, 1354, 1512, 1987, 2050, 2128, 3278
BseLI CCNNNNNNNGG 6 cut(s) 129, 510, 1022, 1259, 2339, 2819
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 2 cut(s) 450, 3271
BseRI GAGGAG 1 cut(s) 1974
BseSI GKGCMC 3 cut(s) 1212, 1578, 1635
BseX3I CGGCCG 1 cut(s) 1739
BseXI GCAGC 8 cut(s) 202, 217, 322, 904, 1468, 1490, 2918, 2921
BseYI CCCAGC 1 cut(s) 1473
BsgI GTGCAG 1 cut(s) 3222
Bsh1285I CGRYCG 1 cut(s) 1742
BshFI GGCC 6 cut(s) 406, 1184, 1576, 1741, 1830, 1972
BshNI GGYRCC 1 cut(s) 1209
BshTI ACCGGT 1 cut(s) 3095
BshVI ATCGAT 1 cut(s) 1401
BsiEI CGRYCG 1 cut(s) 1742
BsiHKAI GWGCWC 3 cut(s) 1248, 1635, 2536
BsiHKCI CYCGRG 2 cut(s) 655, 3286
BsiSI CCGG 3 cut(s) 2298, 2757, 3096
BslFI GGGAC 8 cut(s) 665, 1715, 1865, 1931, 2158, 2355, 2476, 2939
BslI CCNNNNNNNGG 6 cut(s) 129, 510, 1022, 1259, 2339, 2819
BsmAI GTCTC 4 cut(s) 1412, 1512, 1958, 2937
BsmFI GGGAC 8 cut(s) 665, 1715, 1865, 1931, 2158, 2355, 2476, 2939
BsmI GAATGC 4 cut(s) 364, 557, 1164, 3082
BsnI GGCC 6 cut(s) 406, 1184, 1576, 1741, 1830, 1972
BsoBI CYCGRG 2 cut(s) 655, 3286
Bsp120I GGGCCC 1 cut(s) 1574
Bsp1286I GDGCHC 5 cut(s) 1212, 1248, 1578, 1635, 2536
Bsp13I TCCGGA 1 cut(s) 2756
Bsp1407I TGTACA 2 cut(s) 1392, 1686
Bsp19I CCATGG 3 cut(s) 647, 1590, 2207
BspACI CCGC 9 cut(s) 154, 555, 1328, 1360, 1693, 2354, 2591, 2807, 3233
BspANI GGCC 6 cut(s) 406, 1184, 1576, 1741, 1830, 1972
BspCNI CTCAG 2 cut(s) 451, 3270
BspDI ATCGAT 1 cut(s) 1401
BspEI TCCGGA 1 cut(s) 2756
BspLI GGNNCC 7 cut(s) 297, 372, 1211, 1576, 1617, 1651, 2755
BspMI ACCTGC 4 cut(s) 313, 597, 622, 1586
BspPI GGATC 9 cut(s) 69, 86, 174, 437, 894, 1500, 2146, 3135, 3315
BspQI GCTCTTC 2 cut(s) 2707, 3064
BspT107I GGYRCC 1 cut(s) 1209
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 2 cut(s) 2297, 3095
BsrGI TGTACA 2 cut(s) 1392, 1686
BssAI RCCGGY 2 cut(s) 2297, 3095
BssECI CCNNGG 9 cut(s) 127, 647, 777, 978, 1590, 1864, 2207, 2680, 3285
BssT1I CCWWGG 7 cut(s) 127, 647, 777, 978, 1590, 2207, 2680
Bst2UI CCWGG 3 cut(s) 845, 1865, 2970
Bst4CI ACNGT 9 cut(s) 175, 596, 1340, 1626, 2398, 2598, 2855, 3110, 3243
Bst6I CTCTTC 3 cut(s) 2707, 3064, 3117
BstAPI GCANNNNNTGC 1 cut(s) 563
BstAUI TGTACA 2 cut(s) 1392, 1686
BstBAI YACGTR 2 cut(s) 1256, 2816
BstC8I GCNNGC 7 cut(s) 251, 1282, 1567, 1832, 2358, 2532, 3237
BstDEI CTNAG 2 cut(s) 459, 3257
BstDSI CCRYGG 3 cut(s) 647, 1590, 2207
BstEII GGTNACC 2 cut(s) 609, 1925
BstF5I GGATG 9 cut(s) 840, 1098, 1249, 1354, 1512, 1987, 2050, 2128, 3278
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 1 cut(s) 230
BstMAI GTCTC 4 cut(s) 1412, 1512, 1958, 2937
BstMCI CGRYCG 1 cut(s) 1742
BstMWI GCNNNNNNNGC 6 cut(s) 212, 563, 1161, 1450, 1969, 2918
BstNI CCWGG 3 cut(s) 845, 1865, 2970
BstNSI RCATGY 7 cut(s) 1284, 1393, 1536, 1693, 1769, 2950, 2954
BstPI GGTNACC 2 cut(s) 609, 1925
BstSCI CCNGG 3 cut(s) 843, 1863, 2968
BstSFI CTRYAG 4 cut(s) 481, 546, 1665, 2538
BstSLI GKGCMC 3 cut(s) 1212, 1578, 1635
BstV1I GCAGC 8 cut(s) 202, 217, 322, 904, 1468, 1490, 2918, 2921
BstV2I GAAGAC 4 cut(s) 723, 775, 1271, 2304
BstX2I RGATCY 5 cut(s) 91, 166, 886, 2138, 3127
BstXI CCANNNNNNTGG 2 cut(s) 1071, 2526
BstYI RGATCY 5 cut(s) 91, 166, 886, 2138, 3127
BstZI CGGCCG 1 cut(s) 1739
Bsu15I ATCGAT 1 cut(s) 1401
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 6 cut(s) 406, 1184, 1576, 1741, 1830, 1972
BsuTUI ATCGAT 1 cut(s) 1401
BtgI CCRYGG 3 cut(s) 647, 1590, 2207
BtsCI GGATG 9 cut(s) 840, 1098, 1249, 1354, 1512, 1987, 2050, 2128, 3278
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 3 cut(s) 392, 2646, 2846
BveI ACCTGC 4 cut(s) 313, 597, 622, 1586
Cac8I GCNNGC 7 cut(s) 251, 1282, 1567, 1832, 2358, 2532, 3237
CaiI CAGNNNCTG 1 cut(s) 2696
CfoI GCGC 1 cut(s) 230
Cfr10I RCCGGY 2 cut(s) 2297, 3095
Cfr13I GGNCC 7 cut(s) 1574, 1575, 1615, 2204, 2851, 2876, 3163
ClaI ATCGAT 1 cut(s) 1401
Csp6I GTAC 7 cut(s) 142, 427, 592, 943, 1393, 1687, 1801
CspAI ACCGGT 1 cut(s) 3095
CspCI CAANNNNNGTGG 2 cut(s) 1140, 1175
CviQI GTAC 7 cut(s) 142, 427, 592, 943, 1393, 1687, 1801
DdeI CTNAG 2 cut(s) 459, 3257
DraI TTTAAA 2 cut(s) 1189, 2274
DrdI GACNNNNNNGTC 1 cut(s) 2951
DseDI GACNNNNNNGTC 1 cut(s) 2951
EaeI YGGCCR 1 cut(s) 1739
EagI CGGCCG 1 cut(s) 1739
Eam1104I CTCTTC 3 cut(s) 2707, 3064, 3117
EarI CTCTTC 3 cut(s) 2707, 3064, 3117
EciI GGCGGA 1 cut(s) 3222
EclXI CGGCCG 1 cut(s) 1739
Eco130I CCWWGG 7 cut(s) 127, 647, 777, 978, 1590, 2207, 2680
Eco147I AGGCCT 1 cut(s) 1184
Eco24I GRGCYC 1 cut(s) 1578
Eco32I GATATC 1 cut(s) 2764
Eco47I GGWCC 5 cut(s) 1615, 2204, 2851, 2876, 3163
Eco47III AGCGCT 1 cut(s) 229
Eco52I CGGCCG 1 cut(s) 1739
Eco57I CTGAAG 3 cut(s) 972, 1469, 2673
Eco72I CACGTG 2 cut(s) 1256, 2816
Eco81I CCTNAGG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 655, 3286
Eco91I GGTNACC 2 cut(s) 609, 1925
EcoO109I RGGNCCY 1 cut(s) 1615
EcoO65I GGTNACC 2 cut(s) 609, 1925
EcoRI GAATTC 1 cut(s) 2828
EcoRII CCWGG 3 cut(s) 843, 1863, 2968
EcoRV GATATC 1 cut(s) 2764
EcoT14I CCWWGG 7 cut(s) 127, 647, 777, 978, 1590, 2207, 2680
EcoT22I ATGCAT 3 cut(s) 1286, 1538, 2952
EcoT38I GRGCYC 1 cut(s) 1578
ErhI CCWWGG 7 cut(s) 127, 647, 777, 978, 1590, 2207, 2680
FalI AAGNNNNNCTT 6 cut(s) 204, 236, 275, 307, 3114, 3146
FaqI GGGAC 8 cut(s) 665, 1715, 1865, 1931, 2158, 2355, 2476, 2939
FauI CCCGC 2 cut(s) 1367, 1686
FauNDI CATATG 3 cut(s) 1847, 1909, 3049
FbaI TGATCA 1 cut(s) 1723
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 9 cut(s) 216, 231, 336, 918, 1479, 1482, 2592, 2907, 2910
FokI GGATG 9 cut(s) 827, 1085, 1236, 1341, 1519, 1974, 2057, 2135, 3265
FriOI GRGCYC 1 cut(s) 1578
Fsp4HI GCNGC 9 cut(s) 216, 231, 336, 918, 1479, 1482, 2592, 2907, 2910
FspBI CTAG 4 cut(s) 2379, 2568, 3081, 3322
GlaI GCGC 1 cut(s) 229
GluI GCNGC 9 cut(s) 216, 231, 336, 918, 1479, 1482, 2592, 2907, 2910
GsaI CCCAGC 1 cut(s) 1477
GsuI CTGGAG 1 cut(s) 2259
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 6 cut(s) 406, 1184, 1576, 1741, 1830, 1972
HapII CCGG 3 cut(s) 2298, 2757, 3096
HhaI GCGC 1 cut(s) 230
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 2 cut(s) 244, 1861
HindII GTYRAC 2 cut(s) 244, 1861
HindIII AAGCTT 2 cut(s) 995, 2267
HpaII CCGG 3 cut(s) 2298, 2757, 3096
HphI GGTGA 7 cut(s) 94, 163, 1937, 2573, 2761, 3098, 3110
Hpy166II GTNNAC 9 cut(s) 244, 427, 550, 968, 1096, 1234, 1633, 1861, 3214
Hpy8I GTNNAC 9 cut(s) 244, 427, 550, 968, 1096, 1234, 1633, 1861, 3214
HpyAV CCTTC 6 cut(s) 384, 508, 947, 1014, 1634, 2465
HpyCH4III ACNGT 9 cut(s) 175, 596, 1340, 1626, 2398, 2598, 2855, 3110, 3243
HpyCH4IV ACGT 3 cut(s) 162, 1255, 2815
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 563, 1161, 1450, 1969, 2918
HpyF3I CTNAG 2 cut(s) 459, 3257
HpySE526I ACGT 3 cut(s) 162, 1255, 2815
HspAI GCGC 1 cut(s) 228
Kpn2I TCCGGA 1 cut(s) 2756
Ksp22I TGATCA 1 cut(s) 1723
LguI GCTCTTC 2 cut(s) 2707, 3064
LmnI GCTCC 2 cut(s) 301, 2437
Lsp1109I GCAGC 8 cut(s) 202, 217, 322, 904, 1468, 1490, 2918, 2921
LweI GCATC 7 cut(s) 193, 322, 1453, 2652, 2704, 3166, 3287
MaeI CTAG 4 cut(s) 2379, 2568, 3081, 3322
MaeII ACGT 3 cut(s) 162, 1255, 2815
MfeI CAATTG 2 cut(s) 1812, 2773
MflI RGATCY 5 cut(s) 91, 166, 886, 2138, 3127
MhlI GDGCHC 5 cut(s) 1212, 1248, 1578, 1635, 2536
MlyI GAGTC 7 cut(s) 545, 647, 884, 2140, 2222, 2558, 3196
MmeI TCCRAC 5 cut(s) 402, 1580, 2380, 2456, 2920
Mph1103I ATGCAT 3 cut(s) 1286, 1538, 2952
MroI TCCGGA 1 cut(s) 2756
MroXI GAANNNNTTC 3 cut(s) 287, 1660, 2247
MslI CAYNNNNRTG 6 cut(s) 753, 1254, 1394, 2121, 2884, 3152
MspA1I CMGCKG 4 cut(s) 154, 1079, 1362, 1481
MspI CCGG 3 cut(s) 2298, 2757, 3096
MspR9I CCNGG 3 cut(s) 845, 1865, 2970
MunI CAATTG 2 cut(s) 1812, 2773
Mva1269I GAATGC 4 cut(s) 364, 557, 1164, 3082
MvaI CCWGG 3 cut(s) 845, 1865, 2970
MwoI GCNNNNNNNGC 6 cut(s) 212, 563, 1161, 1450, 1969, 2918
NcoI CCATGG 3 cut(s) 647, 1590, 2207
NdeI CATATG 3 cut(s) 1847, 1909, 3049
NlaIV GGNNCC 7 cut(s) 297, 372, 1211, 1576, 1617, 1651, 2755
NmuCI GTSAC 5 cut(s) 100, 1548, 1925, 2561, 2734
NsiI ATGCAT 3 cut(s) 1286, 1538, 2952
NspI RCATGY 7 cut(s) 1284, 1393, 1536, 1693, 1769, 2950, 2954
PaeI GCATGC 1 cut(s) 1284
PceI AGGCCT 1 cut(s) 1184
PciSI GCTCTTC 2 cut(s) 2707, 3064
PctI GAATGC 4 cut(s) 364, 557, 1164, 3082
PdmI GAANNNNTTC 3 cut(s) 287, 1660, 2247
PflMI CCANNNNNTGG 3 cut(s) 510, 1022, 1259
PfoI TCCNGGA 1 cut(s) 2968
PinAI ACCGGT 1 cut(s) 3095
PkrI GCNGC 9 cut(s) 217, 232, 337, 919, 1480, 1483, 2593, 2908, 2911
PleI GAGTC 7 cut(s) 545, 647, 883, 2140, 2222, 2558, 3196
PmaCI CACGTG 2 cut(s) 1256, 2816
PmlI CACGTG 2 cut(s) 1256, 2816
PpsI GAGTC 7 cut(s) 545, 647, 883, 2140, 2222, 2558, 3196
Ppu21I YACGTR 2 cut(s) 1256, 2816
PpuMI RGGWCCY 1 cut(s) 1615
Psp5II RGGWCCY 1 cut(s) 1615
Psp6I CCWGG 3 cut(s) 843, 1863, 2968
PspCI CACGTG 2 cut(s) 1256, 2816
PspEI GGTNACC 2 cut(s) 609, 1925
PspFI CCCAGC 1 cut(s) 1473
PspGI CCWGG 3 cut(s) 843, 1863, 2968
PspN4I GGNNCC 7 cut(s) 297, 372, 1211, 1576, 1617, 1651, 2755
PspOMI GGGCCC 1 cut(s) 1574
PspPI GGNCC 7 cut(s) 1574, 1575, 1615, 2204, 2851, 2876, 3163
PspPPI RGGWCCY 1 cut(s) 1615
PsrI GAACNNNNNNTAC 2 cut(s) 1648, 1680
PstNI CAGNNNCTG 1 cut(s) 2696
PsuI RGATCY 5 cut(s) 91, 166, 886, 2138, 3127
PvuII CAGCTG 2 cut(s) 1079, 1481
RsaI GTAC 7 cut(s) 143, 428, 593, 944, 1394, 1688, 1802
RsaNI GTAC 7 cut(s) 142, 427, 592, 943, 1393, 1687, 1801
RseI CAYNNNNRTG 6 cut(s) 753, 1254, 1394, 2121, 2884, 3152
SapI GCTCTTC 2 cut(s) 2707, 3064
SatI GCNGC 9 cut(s) 216, 231, 336, 918, 1479, 1482, 2592, 2907, 2910
Sau96I GGNCC 7 cut(s) 1574, 1575, 1615, 2204, 2851, 2876, 3163
ScaI AGTACT 1 cut(s) 1802
SchI GAGTC 7 cut(s) 545, 647, 884, 2140, 2222, 2558, 3196
ScrFI CCNGG 3 cut(s) 845, 1865, 2970
SduI GDGCHC 5 cut(s) 1212, 1248, 1578, 1635, 2536
SfaNI GCATC 7 cut(s) 193, 322, 1453, 2652, 2704, 3166, 3287
SfcI CTRYAG 4 cut(s) 481, 546, 1665, 2538
SinI GGWCC 5 cut(s) 1615, 2204, 2851, 2876, 3163
SmiMI CAYNNNNRTG 6 cut(s) 753, 1254, 1394, 2121, 2884, 3152
SmlI CTYRAG 1 cut(s) 1043
SmoI CTYRAG 1 cut(s) 1043
SpeI ACTAGT 1 cut(s) 2378
SphI GCATGC 1 cut(s) 1284
SseBI AGGCCT 1 cut(s) 1184
SsiI CCGC 9 cut(s) 154, 555, 1328, 1360, 1693, 2354, 2591, 2807, 3233
SspMI CTAG 4 cut(s) 2379, 2568, 3081, 3322
StuI AGGCCT 1 cut(s) 1184
StyD4I CCNGG 3 cut(s) 843, 1863, 2968
StyI CCWWGG 7 cut(s) 127, 647, 777, 978, 1590, 2207, 2680
TaaI ACNGT 9 cut(s) 175, 596, 1340, 1626, 2398, 2598, 2855, 3110, 3243
TaiI ACGT 3 cut(s) 165, 1258, 2818
TaqI TCGA 5 cut(s) 751, 1401, 1495, 2579, 3150
TaqII GACCGA 2 cut(s) 1992, 2913
TatI WGTACW 3 cut(s) 1392, 1686, 1800
TauI GCSGC 1 cut(s) 2594
TscAI CASTG 3 cut(s) 392, 2653, 2853
TseFI GTSAC 5 cut(s) 100, 1548, 1925, 2561, 2734
TseI GCWGC 8 cut(s) 215, 230, 335, 917, 1478, 1481, 2906, 2909
Tsp45I GTSAC 5 cut(s) 100, 1548, 1925, 2561, 2734
TspGWI ACGGA 3 cut(s) 112, 2057, 2647
TspRI CASTG 3 cut(s) 392, 2653, 2853
Van91I CCANNNNNTGG 3 cut(s) 510, 1022, 1259
VneI GTGCAC 1 cut(s) 1631
VpaK11BI GGWCC 5 cut(s) 1615, 2204, 2851, 2876, 3163
XapI RAATTY 3 cut(s) 323, 620, 2828
XceI RCATGY 7 cut(s) 1284, 1393, 1536, 1693, 1769, 2950, 2954
XcmI CCANNNNNNNNNTGG 1 cut(s) 1256
XmiI GTMKAC 1 cut(s) 549
XmnI GAANNNNTTC 3 cut(s) 287, 1660, 2247
XspI CTAG 4 cut(s) 2379, 2568, 3081, 3322
ZrmI AGTACT 1 cut(s) 1802
Zsp2I ATGCAT 3 cut(s) 1286, 1538, 2952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.