RchiOBHm_Chr1g0324261

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010440
Physical Location & Seq
Forward (+)
11933990 .. 11947493
13504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55410

Sequence Viewer

Length: 3372 bp
ATGGCTGCTTCTATGATGGGTCAACTGGTTTCTCCCTTAGAGAATGGACATCATGTGTGGGAGGACCAATCTTTCATCAAGTGGAACAAGAGAGATGCACATGTCACTTTACGTTGCCATGAATCAATTGAAGGATCTCTTAAATACTGGTATGAACGCAACAAAGTGAATTTTATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTTCCTGAAGCTCTTGATAGTGCAGCTCTTTGGATCAAGGGCTTGCCTTTTGTGGAGTCTTTGTCAGGCTATTGGAAATTTTACTTAGCTTCCACTCCTGGAAATGTTCCTTTGAATTTTTACCACACTGCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGTTCCTTCAAATTGGCAGATGCATGGATTTGATCGGCCTATATATACTAACGTTGAATATCCATTTCCTCTTGATCCACCTTTTGTTCATGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTCATCCCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTTGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATAACTGATTATTGTAATCCTTGTGGGACGGACAAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGTGATGGCTCTTACCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTTCTTTCCAAGCCACAGACTATTTCTTCAAATCGAATCTGGCTGAGGATTTCTCTTATGCAGACCTACAGGCAAGACTTTATTATTGAGAAGGATCTTTTTGTTTTCAACTTCTTGGTTGAAGTGAAAATAGATGACTCCCAAGAAACATCCAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAGCTGGAACAGCAGTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCACCAAGGACAGTTCTTGGATTTCGTGATTATTTGCTTGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGCCGAATCTGTACACTCTGGTTGTGATATTGAAAGATACATCTGGCAATATTGTGGACTGTGAATCATGCCTAGTAGGCATACGACAAGTATCAAAAGCCCCTAAGCAGCTGCTCGTTAATGGGCATCCAATAACAATAAGGGGTGTGAACAGGCATGAGCACCATCCGCGTCTGGGGAAGACAAATATAGAATCGTGCATGGTTAAGGATTTGGTTTTAATGAAGCAATACAATATCAATGCTGTAAGAAACAGCCATTATCCTCAACATCCCCGCTGGTATGAGCTTTGCGACATGTTTGGCATGTACATGATAGATGAAGCCAATATTGAAACCCATGGTTTCGATTATACTGGACATGTGAAACATCCGACTTTGGAACCCAGTTGGGCAACTGCAATGTTGGACCGTGTGATGGGCATGGTTGAGAGGGACAAAAATCATGCGTGCATTATCTCTTGGTCCTTGGGAAATGAATCTGGATATGGACCTAATCATTCTGCTTTAGCTGGTTGGGTTCGTGGAAAGGATCCCTCACGGCTGTTACATTACGAAGGGGGAGGATCCAGAACCCCGTCTACAGATATTATATGCCCCATGTATATGCGTGTTTGGGACATTGTGAAGATTGCAAAAGATCCAAATGAAACACGTCCTCTAATACTGTGCGAGTATTCACATGCAATGGGAAATAGCAATGGAAATATACATGAATATTGGGAAGCAATTGAAGGCACATTTGGTCTCCAAGGAGGCTTTATCTGGGAGTGGGTTGATCAGGGCTTACTGAAAGATAGTGCTGATGGTACTAAACACTGGGCATATGGTGGTGACTTTGGTGACGTTCCTAATGATTTAAACTTCTGTTTGAATGGCCTTGTGTGGCCCGATCGAACTCCTCATCCTGCAATGCATGAAGTGAAGTATGTGTACCAACCAATCAAGGTTTCATTTAGTGAAGGAACACTAAAGATAACAAATACTCACTTTTACGAAACAACAAAAGCATTGGAATTCAGCTGGGCTGCTCATGGAGATGGATGTGAACTTGGATCTGGAAATTTATCCCTTCCCTTGATTGAACCCCAGAAGACTTTTCATATTGAGTTGCAGTCAGCTCCGTGGCATACTCTATGGGCTTCATCATCAGCAGAGGAGTTTTTCTTAACAATAAGTGCTAAGCTTTTGCATGCCACATGCTGGGTTGAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTGCCAAAAGAGAATTTGTTCCTCATGTCATCAAGACTAAAGATACGACATTCCTTAAGGAAATTCTTGGAGATACACTTAAAGTTGGCCAACAGAATGCCTGGGAGATTATACTGAATCTTAAAATGGGAACAGTTGAAAGCTGGAAGGTGGAAGGAGTTCCTTTGATGACGAAAGGCATATTTCCATGTTTTTGGCGAGCACCCACAGATAATGACAAAGGCGGAGGTGCCAGTAGTTATTTGTCCAAGTGGCAAGCTGCGCATATTGATAACCTTCATTATATTACCAAGAGCTGTTCTGTACAGAATATGACTGACCATCTTGTGAAAGTAGCTGTTGTTTTTCTCGGTGTTCCAAACAGTGGGGACGGTTCTGGGGTGGAAGACACAAATGCCTTAATTGAAATTGATGTGATTTACACAATCTACGGTTCAGGGGATGTTGTTGTTGAATGCAATGTTAGACCAAGTTCGAACCTTCCTCCTCTGCCACGTGTGGGAGTTGAGTTCCACCTGGAGAAGTCAATAGACCAGATTAAATGGTACGGAAGAGGACCATTTGAATGTTATCCAGACAGAAAAGCAGCTGCTCATGTTGGAGTCTATGAGCAGAAAGTGAGTGATCTGCATGTCCCTTATATAGTTCCTGGGGAATGTTCAGGTAGGGCAGATGTTCGGTGGGTGACATTTCAAAACAAGGACGGTTTGGGAATCTATGCTTCAATATATGGCAGCTCTCCACCCATGCAATTAAATGCAAGTTATTACACCACAGCTGAGCTGGATCGGGCAACACACAATGAAGATCTTATCAGAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTGGCTGGGGATGATAGTTGGTCTCCATGTGTCCATGACAAGTATCTGATACCTCCTGTTCCAAGCTCATTCTCAATTAGGCTCTCTCCAATAACTGCTGCAACCTCTGGCCATGATATCTACAAATCTCAACTTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1123

Amino Acids

126.61

Weight (kDa)

5.64

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 260 3.7e-54 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 303 - 402 1.1e-08 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 409 - 689 2e-97 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 697 - 790 1.3e-18 Beta-galactosidase, domain 4
Bgal_small_N PF02929 821 - 1104 8.8e-73 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2631
AccB1I GGYRCC 1 cut(s) 2597
AccB7I CCANNNNNTGG 3 cut(s) 513, 2322, 2732
AccI GTMKAC 2 cut(s) 610, 1700
AccII CGCG 1 cut(s) 1291
AciI CCGC 3 cut(s) 1289, 1396, 2592
AclI AACGTT 1 cut(s) 423
AcoI YGGCCR 2 cut(s) 2455, 3337
AcsI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2135, 2182, 2381, 2430
AcuI CTGAAG 1 cut(s) 237
AcvI CACGTG 1 cut(s) 2864
AfaI GTAC 7 cut(s) 675, 1133, 1430, 1930, 2054, 2673, 2915
AfiI CCNNNNNNNGG 7 cut(s) 513, 1295, 1537, 2322, 2732, 2867, 3235
AflII CTTAAG 1 cut(s) 2423
AflIII ACRYGT 5 cut(s) 100, 1416, 1480, 1772, 2863
AjiI CACGTC 1 cut(s) 1775
AjnI CCWGG 5 cut(s) 307, 2468, 2883, 3016, 3228
AjuI GAANNNNNNNTTGG 4 cut(s) 1845, 1877, 2053, 2085
AloI GAACNNNNNNTCC 2 cut(s) 2008, 2040
Alw21I GWGCWC 2 cut(s) 1284, 2572
Alw26I GTCTC 3 cut(s) 1081, 1871, 3255
AoxI GGCC 6 cut(s) 407, 1996, 2006, 2455, 3226, 3337
ApoI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2135, 2182, 2381, 2430
Asp700I GAANNNNTTC 2 cut(s) 2385, 2526
AspLEI GCGC 1 cut(s) 2632
AspS9I GGNCC 7 cut(s) 64, 1528, 1584, 1610, 2007, 2924, 3226
AsuHPI GGTGA 4 cut(s) 1035, 1964, 1973, 3064
AsuII TTCGAA 1 cut(s) 2843
AvaII GGWCC 5 cut(s) 64, 1528, 1584, 1610, 2924
BalI TGGCCA 2 cut(s) 2457, 3339
BamHI GGATCC 2 cut(s) 1651, 1685
BanI GGYRCC 1 cut(s) 2597
BarI GAAGNNNNNNTAC 2 cut(s) 2036, 2068
BbrPI CACGTG 1 cut(s) 2864
BbsI GAAGAC 3 cut(s) 1307, 2219, 2760
Bbv12I GWGCWC 2 cut(s) 1284, 2572
BbvCI CCTCAGC 1 cut(s) 809
BccI CCATC 8 cut(s) 10, 690, 698, 1293, 1531, 1919, 2153, 2697
BceAI ACGGC 1 cut(s) 1676
BciT130I CCWGG 5 cut(s) 309, 2470, 2885, 3018, 3230
BciVI GTATCC 1 cut(s) 190
BclI TGATCA 2 cut(s) 1897, 3211
BcoDI GTCTC 3 cut(s) 1081, 1871, 3255
BfaI CTAG 2 cut(s) 677, 1193
BfmI CTRYAG 7 cut(s) 484, 588, 832, 945, 1113, 1701, 3368
BfrI CTTAAG 1 cut(s) 2423
BfuI GTATCC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 1197
BglII AGATCT 1 cut(s) 3175
BlpI GCTNAGC 2 cut(s) 2301, 3147
Bme1390I CCNGG 5 cut(s) 309, 2470, 2885, 3018, 3230
Bme18I GGWCC 5 cut(s) 64, 1528, 1584, 1610, 2924
BmgBI CACGTC 1 cut(s) 1775
BmgT120I GGNCC 7 cut(s) 64, 1528, 1584, 1610, 2007, 2924, 3226
BmiI GGNNCC 4 cut(s) 1503, 1653, 1687, 2599
BmrFI CCNGG 5 cut(s) 309, 2470, 2885, 3018, 3230
BmrI ACTGGG 2 cut(s) 1500, 1948
BmsI GCATC 4 cut(s) 85, 196, 381, 1255
BmuI ACTGGG 2 cut(s) 1500, 1948
BpiI GAAGAC 3 cut(s) 1307, 2219, 2760
BplI GAGNNNNNCTC 2 cut(s) 802, 834
BpmI CTGGAG 1 cut(s) 2906
Bpu10I CCTNAGC 2 cut(s) 809, 1224
Bpu1102I GCTNAGC 2 cut(s) 2301, 3147
Bpu14I TTCGAA 1 cut(s) 2843
BsaAI YACGTR 1 cut(s) 2864
BsaBI GATNNNNATC 1 cut(s) 726
BsaI GGTCTC 2 cut(s) 1871, 3255
BsaJI CCNNGG 9 cut(s) 194, 1046, 1100, 1459, 1587, 1870, 2243, 2469, 3017
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 573, 603
Bsc4I CCNNNNNNNGG 7 cut(s) 513, 1295, 1537, 2322, 2732, 2867, 3235
Bse3DI GCAATG 5 cut(s) 1527, 1812, 1825, 2037, 2833
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 5 cut(s) 309, 2470, 2885, 3018, 3230
BseDI CCNNGG 9 cut(s) 194, 1046, 1100, 1459, 1587, 1870, 2243, 2469, 3017
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 7 cut(s) 513, 1295, 1537, 2322, 2732, 2867, 3235
BseMI GCAATG 5 cut(s) 1527, 1812, 1825, 2037, 2833
BseMII CTCAG 3 cut(s) 345, 800, 3138
BseRI GAGGAG 4 cut(s) 2010, 2291, 2844, 3201
BseYI CCCAGC 3 cut(s) 2142, 2322, 3233
BsgI GTGCAG 2 cut(s) 174, 252
Bsh1236I CGCG 1 cut(s) 1291
Bsh1285I CGRYCG 1 cut(s) 2014
BshFI GGCC 6 cut(s) 409, 1998, 2008, 2457, 3228, 3339
BshNI GGYRCC 1 cut(s) 2597
BsiEI CGRYCG 1 cut(s) 2014
BsiHKAI GWGCWC 2 cut(s) 1284, 2572
BslFI GGGAC 5 cut(s) 670, 1568, 1751, 2750, 2987
BslI CCNNNNNNNGG 7 cut(s) 513, 1295, 1537, 2322, 2732, 2867, 3235
BsmAI GTCTC 3 cut(s) 1081, 1871, 3255
BsmFI GGGAC 5 cut(s) 670, 1568, 1751, 2750, 2987
BsmI GAATGC 3 cut(s) 747, 2470, 2828
BsnI GGCC 6 cut(s) 409, 1998, 2008, 2457, 3228, 3339
Bso31I GGTCTC 2 cut(s) 1871, 3255
Bsp119I TTCGAA 1 cut(s) 2843
Bsp1286I GDGCHC 2 cut(s) 1284, 2572
Bsp1407I TGTACA 3 cut(s) 1131, 1428, 2671
Bsp1720I GCTNAGC 2 cut(s) 2301, 3147
Bsp19I CCATGG 1 cut(s) 1459
BspACI CCGC 3 cut(s) 1289, 1396, 2592
BspANI GGCC 6 cut(s) 409, 1998, 2008, 2457, 3228, 3339
BspCNI CTCAG 3 cut(s) 346, 801, 3139
BspFNI CGCG 1 cut(s) 1291
BspLI GGNNCC 4 cut(s) 1503, 1653, 1687, 2599
BspMAI CTGCAG 1 cut(s) 1117
BspT104I TTCGAA 1 cut(s) 2843
BspT107I GGYRCC 1 cut(s) 2597
BspTI CTTAAG 1 cut(s) 2423
BspTNI GGTCTC 2 cut(s) 1871, 3255
BsrDI GCAATG 5 cut(s) 1527, 1812, 1825, 2037, 2833
BsrGI TGTACA 3 cut(s) 1131, 1428, 2671
BssECI CCNNGG 9 cut(s) 194, 1046, 1100, 1459, 1587, 1870, 2243, 2469, 3017
BssT1I CCWWGG 6 cut(s) 194, 1046, 1100, 1459, 1587, 1870
Bst2UI CCWGG 5 cut(s) 309, 2470, 2885, 3018, 3230
Bst6I CTCTTC 1 cut(s) 2914
BstAFI CTTAAG 1 cut(s) 2423
BstAUI TGTACA 3 cut(s) 1131, 1428, 2671
BstBAI YACGTR 1 cut(s) 2864
BstBI TTCGAA 1 cut(s) 2843
BstC8I GCNNGC 6 cut(s) 254, 1570, 2313, 2370, 2568, 2625
BstDEI CTNAG 7 cut(s) 37, 295, 354, 809, 1224, 2301, 3147
BstDSI CCRYGG 2 cut(s) 1459, 2243
BstFNI CGCG 1 cut(s) 1291
BstHHI GCGC 1 cut(s) 2632
BstMAI GTCTC 3 cut(s) 1081, 1871, 3255
BstMCI CGRYCG 1 cut(s) 2014
BstMWI GCNNNNNNNGC 5 cut(s) 566, 981, 1197, 1288, 2629
BstNI CCWGG 5 cut(s) 309, 2470, 2885, 3018, 3230
BstNSI RCATGY 8 cut(s) 104, 1420, 1429, 1484, 1805, 2315, 2322, 3002
BstSCI CCNGG 5 cut(s) 307, 2468, 2883, 3016, 3228
BstSFI CTRYAG 7 cut(s) 484, 588, 832, 945, 1113, 1701, 3368
BstUI CGCG 1 cut(s) 1291
BstV2I GAAGAC 3 cut(s) 1307, 2219, 2760
BstX2I RGATCY 8 cut(s) 134, 859, 992, 1651, 1685, 1759, 2174, 3175
BstXI CCANNNNNNTGG 2 cut(s) 1107, 2562
BstYI RGATCY 8 cut(s) 134, 859, 992, 1651, 1685, 1759, 2174, 3175
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 6 cut(s) 409, 1998, 2008, 2457, 3228, 3339
BtgI CCRYGG 2 cut(s) 1459, 2243
BtrI CACGTC 1 cut(s) 1775
BtsI GCAGTG 2 cut(s) 336, 991
BtsIMutI CAGTG 6 cut(s) 336, 931, 973, 991, 1936, 2737
Cac8I GCNNGC 6 cut(s) 254, 1570, 2313, 2370, 2568, 2625
CfoI GCGC 1 cut(s) 2632
Cfr13I GGNCC 7 cut(s) 64, 1528, 1584, 1610, 2007, 2924, 3226
CseI GACGC 1 cut(s) 1280
Csp6I GTAC 7 cut(s) 674, 1132, 1429, 1929, 2053, 2672, 2914
CviQI GTAC 7 cut(s) 674, 1132, 1429, 1929, 2053, 2672, 2914
DdeI CTNAG 7 cut(s) 37, 295, 354, 809, 1224, 2301, 3147
DraI TTTAAA 1 cut(s) 1980
EaeI YGGCCR 2 cut(s) 2455, 3337
Eam1104I CTCTTC 1 cut(s) 2914
EarI CTCTTC 1 cut(s) 2914
EciI GGCGGA 1 cut(s) 2607
Eco130I CCWWGG 6 cut(s) 194, 1046, 1100, 1459, 1587, 1870
Eco31I GGTCTC 2 cut(s) 1871, 3255
Eco32I GATATC 1 cut(s) 3346
Eco47I GGWCC 5 cut(s) 64, 1528, 1584, 1610, 2924
Eco57I CTGAAG 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 2864
EcoRI GAATTC 1 cut(s) 2135
EcoRII CCWGG 5 cut(s) 307, 2468, 2883, 3016, 3228
EcoRV GATATC 1 cut(s) 3346
EcoT14I CCWWGG 6 cut(s) 194, 1046, 1100, 1459, 1587, 1870
EcoT22I ATGCAT 2 cut(s) 396, 2037
ErhI CCWWGG 6 cut(s) 194, 1046, 1100, 1459, 1587, 1870
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 5 cut(s) 670, 1568, 1751, 2750, 2987
FauI CCCGC 1 cut(s) 1403
FauNDI CATATG 1 cut(s) 1945
FbaI TGATCA 2 cut(s) 1897, 3211
FblI GTMKAC 2 cut(s) 610, 1700
FspBI CTAG 2 cut(s) 677, 1193
FspI TGCGCA 1 cut(s) 2631
GlaI GCGC 1 cut(s) 2631
GsaI CCCAGC 3 cut(s) 2146, 2326, 3237
GsuI CTGGAG 1 cut(s) 2906
HaeIII GGCC 6 cut(s) 409, 1998, 2008, 2457, 3228, 3339
HgaI GACGC 1 cut(s) 1280
HhaI GCGC 1 cut(s) 2632
Hin6I GCGC 1 cut(s) 2630
HinP1I GCGC 1 cut(s) 2630
HincII GTYRAC 2 cut(s) 23, 611
HindII GTYRAC 2 cut(s) 23, 611
HindIII AAGCTT 2 cut(s) 1031, 2303
HphI GGTGA 4 cut(s) 1035, 1964, 1973, 3064
Hpy166II GTNNAC 9 cut(s) 23, 466, 611, 1134, 1177, 1270, 1701, 2053, 2168
Hpy188I TCNGA 4 cut(s) 355, 1494, 3185, 3276
Hpy8I GTNNAC 9 cut(s) 23, 466, 611, 1134, 1177, 1270, 1701, 2053, 2168
HpyCH4IV ACGT 5 cut(s) 112, 423, 1774, 1965, 2863
HpyF10VI GCNNNNNNNGC 5 cut(s) 566, 981, 1197, 1288, 2629
HpyF3I CTNAG 7 cut(s) 37, 295, 354, 809, 1224, 2301, 3147
HpySE526I ACGT 5 cut(s) 112, 423, 1774, 1965, 2863
HspAI GCGC 1 cut(s) 2630
Ksp22I TGATCA 2 cut(s) 1897, 3211
LmnI GCTCC 2 cut(s) 972, 2245
LweI GCATC 4 cut(s) 85, 196, 381, 1255
MaeI CTAG 2 cut(s) 677, 1193
MaeII ACGT 5 cut(s) 112, 423, 1774, 1965, 2863
MaeIII GTNAC 5 cut(s) 103, 1665, 1952, 1961, 3052
MboII GAAGA 9 cut(s) 731, 758, 783, 1312, 1759, 2224, 2765, 2931, 3185
MfeI CAATTG 2 cut(s) 126, 1848
MflI RGATCY 8 cut(s) 134, 859, 992, 1651, 1685, 1759, 2174, 3175
MhlI GDGCHC 2 cut(s) 1284, 2572
MlsI TGGCCA 2 cut(s) 2457, 3339
MluNI TGGCCA 2 cut(s) 2457, 3339
MlyI GAGTC 3 cut(s) 275, 896, 2979
MmeI TCCRAC 4 cut(s) 207, 1506, 1517, 2947
Mox20I TGGCCA 2 cut(s) 2457, 3339
Mph1103I ATGCAT 2 cut(s) 396, 2037
MroXI GAANNNNTTC 2 cut(s) 2385, 2526
MscI TGGCCA 2 cut(s) 2457, 3339
MslI CAYNNNNRTG 5 cut(s) 756, 1430, 1724, 2157, 2932
Msp20I TGGCCA 2 cut(s) 2457, 3339
MspA1I CMGCKG 5 cut(s) 1231, 1398, 2142, 2957, 3146
MspCI CTTAAG 1 cut(s) 2423
MspR9I CCNGG 5 cut(s) 309, 2470, 2885, 3018, 3230
MunI CAATTG 2 cut(s) 126, 1848
Mva1269I GAATGC 3 cut(s) 747, 2470, 2828
MvaI CCWGG 5 cut(s) 309, 2470, 2885, 3018, 3230
MvnI CGCG 1 cut(s) 1291
MwoI GCNNNNNNNGC 5 cut(s) 566, 981, 1197, 1288, 2629
NcoI CCATGG 1 cut(s) 1459
NdeI CATATG 1 cut(s) 1945
NlaIV GGNNCC 4 cut(s) 1503, 1653, 1687, 2599
NmuCI GTSAC 4 cut(s) 103, 1952, 1961, 3052
NsbI TGCGCA 1 cut(s) 2631
NsiI ATGCAT 2 cut(s) 396, 2037
NspI RCATGY 8 cut(s) 104, 1420, 1429, 1484, 1805, 2315, 2322, 3002
NspV TTCGAA 1 cut(s) 2843
PaeI GCATGC 1 cut(s) 2315
PciI ACATGT 3 cut(s) 100, 1416, 1480
PctI GAATGC 3 cut(s) 747, 2470, 2828
PdmI GAANNNNTTC 2 cut(s) 2385, 2526
PflMI CCANNNNNTGG 3 cut(s) 513, 2322, 2732
PfoI TCCNGGA 1 cut(s) 307
Ple19I CGATCG 1 cut(s) 2014
PleI GAGTC 3 cut(s) 274, 896, 2978
PmaCI CACGTG 1 cut(s) 2864
PmlI CACGTG 1 cut(s) 2864
PpsI GAGTC 3 cut(s) 274, 896, 2978
Ppu21I YACGTR 1 cut(s) 2864
PscI ACATGT 3 cut(s) 100, 1416, 1480
Psp1406I AACGTT 1 cut(s) 423
Psp6I CCWGG 5 cut(s) 307, 2468, 2883, 3016, 3228
PspCI CACGTG 1 cut(s) 2864
PspFI CCCAGC 3 cut(s) 2142, 2322, 3233
PspGI CCWGG 5 cut(s) 307, 2468, 2883, 3016, 3228
PspN4I GGNNCC 4 cut(s) 1503, 1653, 1687, 2599
PspPI GGNCC 7 cut(s) 64, 1528, 1584, 1610, 2007, 2924, 3226
PsrI GAACNNNNNNTAC 4 cut(s) 666, 698, 1684, 1716
PstI CTGCAG 1 cut(s) 1117
PsuI RGATCY 8 cut(s) 134, 859, 992, 1651, 1685, 1759, 2174, 3175
PvuI CGATCG 1 cut(s) 2014
PvuII CAGCTG 4 cut(s) 1231, 2142, 2957, 3146
RsaI GTAC 7 cut(s) 675, 1133, 1430, 1930, 2054, 2673, 2915
RsaNI GTAC 7 cut(s) 674, 1132, 1429, 1929, 2053, 2672, 2914
RseI CAYNNNNRTG 5 cut(s) 756, 1430, 1724, 2157, 2932
SalI GTCGAC 1 cut(s) 609
Sau96I GGNCC 7 cut(s) 64, 1528, 1584, 1610, 2007, 2924, 3226
SchI GAGTC 3 cut(s) 275, 896, 2979
ScrFI CCNGG 5 cut(s) 309, 2470, 2885, 3018, 3230
SduI GDGCHC 2 cut(s) 1284, 2572
SfaNI GCATC 4 cut(s) 85, 196, 381, 1255
SfcI CTRYAG 7 cut(s) 484, 588, 832, 945, 1113, 1701, 3368
SfuI TTCGAA 1 cut(s) 2843
SinI GGWCC 5 cut(s) 64, 1528, 1584, 1610, 2924
SmiMI CAYNNNNRTG 5 cut(s) 756, 1430, 1724, 2157, 2932
SmlI CTYRAG 1 cut(s) 2423
SmoI CTYRAG 1 cut(s) 2423
SphI GCATGC 1 cut(s) 2315
SsiI CCGC 3 cut(s) 1289, 1396, 2592
SspI AATATT 3 cut(s) 1171, 1450, 1838
SspMI CTAG 2 cut(s) 677, 1193
StyD4I CCNGG 5 cut(s) 307, 2468, 2883, 3016, 3228
StyI CCWWGG 6 cut(s) 194, 1046, 1100, 1459, 1587, 1870
TaiI ACGT 5 cut(s) 115, 426, 1777, 1968, 2866
TaqI TCGA 6 cut(s) 552, 610, 799, 1467, 2014, 2843
TatI WGTACW 4 cut(s) 673, 1131, 1428, 2671
TscAI CASTG 6 cut(s) 343, 931, 973, 991, 1943, 2737
TseFI GTSAC 4 cut(s) 103, 1952, 1961, 3052
Tsp45I GTSAC 4 cut(s) 103, 1952, 1961, 3052
TspGWI ACGGA 3 cut(s) 674, 2232, 2931
TspRI CASTG 6 cut(s) 343, 931, 973, 991, 1943, 2737
Van91I CCANNNNNTGG 3 cut(s) 513, 2322, 2732
Vha464I CTTAAG 1 cut(s) 2423
VpaK11BI GGWCC 5 cut(s) 64, 1528, 1584, 1610, 2924
XapI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2135, 2182, 2381, 2430
XceI RCATGY 8 cut(s) 104, 1420, 1429, 1484, 1805, 2315, 2322, 3002
XcmI CCANNNNNNNNNTGG 1 cut(s) 3148
XmiI GTMKAC 2 cut(s) 610, 1700
XmnI GAANNNNTTC 2 cut(s) 2385, 2526
XspI CTAG 2 cut(s) 677, 1193
Zsp2I ATGCAT 2 cut(s) 396, 2037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.