FvH4_7g03060

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010440
Physical Location & Seq
Forward (+)
3419482 .. 3431189
11708 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03060.t2

Sequence Viewer

Length: 2949 bp
ATGCACGGATTTGATCGGCCTATATATACAAATGTTGTATATCCTTTTCCACTTGATCCACCTTTTGTTCCTGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTTATCCCTGAAGAATGGAAGGGTCGCAGAGTTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATTAATGGAGTCCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATCACTGATTATTGCTATCCTTGTGGTTCGGACAAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGCGATGGCTCTTATCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTCCTTCTTTCCAAGCCACAGGTATTTATTGGAGACTATTTCTTCAGATCAAATCTGGCTGAGGATTTCTCCTATGCAGATTTACAGGTTGAAGTGAAAATAGATAACTCCCGAGAAACATCTAAAAACACTGTTATTGATAATTTCACTATAGAAGCTGCGTTATTTGACAGTGGAAGCTGGTACAGCATTGGTGGATCTGCTGACCTGCTATCATCAAATGTAGCTAATCTAAAGCTTGATCTTTCACCTGGCTCCATTCTAGGATTCCGTGATTATTCGCTGGTAGGGAGACTGGAAGCGCCAAGGCTTTGGTCTGCAGAGCAGCCGAATCTCTACACTCTGGTTGTGATATTGAAAGATAAATCTGGCAACATTGTGGACTGTGAATCGTGTGTAGTAGGCATACGACAAGTATCAAATGCCCCTAAGCAGCTGCTTGTTAATGGGCATCCGATAATAATAAGGGGTGTGAACAGGCATGAGCATCATCCACGTCTGGGGAAGACAAACATAGAATCGTGCATGATTAAGGATTTGGTTTTAATGAAGCAATACAACATCAATGCTGTAAGAAACAGCCATTATCCTCAACATCCCCGCTGGTATGAGCTTTGTGACATATTTGGCATGTACATGATAGATGAAGCCAATATTGAAGCCCATGGTTTCGACTATTCTGGACATGTGAAGCATCCGACTTTGGAACCCAGTTGGGCAACTGCAATGTTAGACCGTGTGATAGGCATGGTTGAGAGGGACAAAAATCATGCATGCATTATCTCTTGGTCCTTGGGAAATGAGTCTGGATATGGACCTAATCATTCTGCTTCAGCTGGTTGGGTTCGTGGAAAGGATCCTTCACGGCTGTTGCATTATGAAGGGGGAGGATCCAGAACCCCGTCTACCGATATTATATGCCCCATGTATATGCGTGTTTGGGACATTGTGAAGATTGCAAAAGATCCAAATGAAACACGTCCTTTAATATTGTGCGAGTATTCACATGCAATGGGCAATAGCAATGGAAATATACATGAATATTGGGAAGCAATTGATAGCACATTTGGTCTCCAAGGTGGCTTTATCTGGGATTGGGTTGATCAGGGCTTACTGAAAGATAGTGCTGATGGTACTAAACACTGGGCATATGGTGGTGACTTTGGTGATGTTCCTAATGATTTAAACTTCTGTCTGAATGGTCTTGTGTGGCCTGATCGAACTCCTCATCCTGCAATGCATGAAGTGAAGTATGTGTACCAGCCAATCAAGGTTTCATTTAGTGAAGGAACACTAAAGGTTACAAATACTCACTTTTATGAAACAACAAGAGCATTGGAATTCTACTGGGCTGCTCATGGAGATGGATGTGAACTTGGATCTGGAAATTTATCCCTTCCCTTGATTGAACCCCAGAAGACTTATCATATTGAGTCGCAGTCAGCTCCGTGGCATACTCTATGGGCTTCATCATCAGCAGAGGAGTTTTTCTTAACCATAACTGCTAAGCTTTTGCACTCTACATGCTGGGTTGAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTGTTAAAAGAGAATTTGTTCCTCATGTCATCAAGACTAAAGATGCTACATTCCTTAGGGAAATTGTTGGAGATACACTTAAAGTCAGCCAACAGAATGCCTGGGAGATAATACTGAATGTTAAAATGGGTACAGTTGAAAGCTGGAAGGTTGAAGGAGTTCCTTTGATGACAAAAGGCATATTTCCATGTTTCTGGCGAGCACCGACAGATAATGACAAAGGGGGAGGTGCCAGTAGTTATTCCTCCAAGTGGCAAGCTGCTCATATTGATAACCTTCATTACATTACCAAGAGCTGTTCTGTGGAGAATATGAGTGACGATCTTCTGAAAGTAGCTGTTGTGTTTCTCGGTGTTCCAAACAGTGGGGAGGGTTCTGGGGTGGAAGACAGAAGTGCCTTGATCGAAATCGATGTGATATACACAATTTACAGTTCGGGGGACGTTGTTGTCGAGTGCAATGTCAGACCAAATTCGAACCTTCCGCCTCTGCCACGTGTGGGAGTTGAGTTTCACTTGGAGAAGTCAATAGACCAGATTAAATGGTACGGAAGAGGACCATTTGAATGTTATCCAGACAGAAAAGTAGCTGCTCATGTTGGAGTCTATGAGCAGAAAGTGGGTGACCTGCATGTCCCTTATATAGTTCCTGGGGAATGTTCAGGTAGGGCGGACGTTAGATGGGTGACATTCCAAAACAAGGATGGTTTGGGAATCTATGCTTCAATATATGGCAGCTCTCCACCCATGCAAATGAATGCAAGTTACTACACCACAGCTGAGCTGGATCGAGCAACTCACAATGAAGATCTTATCAGAGGAGATGACATTGAGGTTCATCTCGATCACAAGCACATGGGGCTGGCCGGGGATGACAGTTGGTCTCCATGTGTCCATGACAAGTATCTAATTCCTGCTGTGCCAAGCTCATTCTCAATTAGGCTTTCTCCAATAACCCCTGCAACCTCTGGCCATGATATCTACAAATCTCAAGTTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

983

Amino Acids

110.17

Weight (kDa)

5.47

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 90 - 261 6.6e-54 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 263 - 392 2.1e-13 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 399 - 679 1.1e-95 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 687 - 780 6e-17 Beta-galactosidase, domain 4
Bgal_small_N PF02929 811 - 1094 8.1e-72 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2393, 2576
Acc36I ACCTGC 2 cut(s) 588, 2580
AccB1I GGYRCC 1 cut(s) 2174
AccB7I CCANNNNNTGG 1 cut(s) 2309
AccI GTMKAC 2 cut(s) 220, 1277
AciI CCGC 3 cut(s) 973, 2429, 2615
AclWI GGATC 9 cut(s) 50, 577, 1223, 1236, 1257, 1270, 1331, 1759, 2739
AcoI YGGCCR 2 cut(s) 2808, 2914
AcsI RAATTY 5 cut(s) 233, 1712, 1759, 1958, 2416
AcuI CTGAAG 3 cut(s) 138, 400, 1188
AcvI CACGTG 1 cut(s) 2441
AfaI GTAC 7 cut(s) 285, 557, 1007, 1507, 1631, 2077, 2492
AfiI CCNNNNNNNGG 5 cut(s) 872, 2196, 2309, 2444, 2644
AflIII ACRYGT 3 cut(s) 1057, 1349, 2440
AhdI GACNNNNNGTC 1 cut(s) 2824
AjiI CACGTC 2 cut(s) 869, 1352
AjnI CCWGG 3 cut(s) 622, 2045, 2593
AjuI GAANNNNNNNTTGG 2 cut(s) 1630, 1662
AloI GAACNNNNNNTCC 2 cut(s) 1585, 1617
Alw21I GWGCWC 2 cut(s) 375, 2149
Alw26I GTCTC 4 cut(s) 399, 658, 1448, 2832
AlwI GGATC 9 cut(s) 50, 577, 1223, 1236, 1257, 1270, 1331, 1759, 2739
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 4 cut(s) 17, 1583, 2808, 2914
ApeKI GCWGC 9 cut(s) 90, 530, 697, 805, 808, 1724, 2204, 2534, 2679
ApoI RAATTY 5 cut(s) 233, 1712, 1759, 1958, 2416
AseI ATTAAT 1 cut(s) 186
Asp700I GAANNNNTTC 2 cut(s) 1962, 2103
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 3 cut(s) 1161, 1187, 2501
AsuC2I CCSGG 1 cut(s) 2812
AsuHPI GGTGA 5 cut(s) 612, 1541, 1550, 2579, 2641
AsuII TTCGAA 2 cut(s) 153, 2420
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 3 cut(s) 1161, 1187, 2501
AxyI CCTNAGG 1 cut(s) 2000
BalI TGGCCA 1 cut(s) 2916
BamHI GGATCC 2 cut(s) 1228, 1262
BanI GGYRCC 1 cut(s) 2174
BarI GAAGNNNNNNTAC 2 cut(s) 1613, 1645
BbrPI CACGTG 1 cut(s) 2441
BbsI GAAGAC 3 cut(s) 884, 1796, 2337
Bbv12I GWGCWC 2 cut(s) 375, 2149
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 9 cut(s) 77, 517, 709, 795, 817, 1711, 2191, 2521, 2691
BccI CCATC 6 cut(s) 300, 308, 1496, 1730, 2619, 2642
BceAI ACGGC 1 cut(s) 1253
BciT130I CCWGG 3 cut(s) 624, 2047, 2595
BclI TGATCA 1 cut(s) 1474
BcnI CCSGG 1 cut(s) 2812
BcoDI GTCTC 4 cut(s) 399, 658, 1448, 2832
BfaI CTAG 2 cut(s) 287, 635
BfmI CTRYAG 5 cut(s) 94, 198, 522, 690, 2945
BfoI RGCGCY 1 cut(s) 677
BfuAI ACCTGC 2 cut(s) 588, 2580
BglII AGATCT 1 cut(s) 2752
BisI GCNGC 9 cut(s) 91, 531, 698, 806, 809, 1725, 2205, 2535, 2680
BlpI GCTNAGC 2 cut(s) 1878, 2724
BlsI GCNGC 9 cut(s) 92, 532, 699, 807, 810, 1726, 2206, 2536, 2681
Bme1390I CCNGG 4 cut(s) 624, 2047, 2595, 2812
Bme18I GGWCC 3 cut(s) 1161, 1187, 2501
BmeRI GACNNNNNGTC 1 cut(s) 2824
BmeT110I CYCGRG 1 cut(s) 483
BmgBI CACGTC 2 cut(s) 869, 1352
BmgT120I GGNCC 3 cut(s) 1161, 1187, 2501
BmiI GGNNCC 5 cut(s) 628, 1080, 1230, 1264, 2176
BmrFI CCNGG 4 cut(s) 624, 2047, 2595, 2812
BmrI ACTGGG 3 cut(s) 1077, 1525, 1729
BmsI GCATC 4 cut(s) 832, 868, 1075, 1978
BmuI ACTGGG 3 cut(s) 1077, 1525, 1729
BpiI GAAGAC 3 cut(s) 884, 1796, 2337
BplI GAGNNNNNCTC 2 cut(s) 425, 457
Bpu10I CCTNAGC 2 cut(s) 432, 801
Bpu1102I GCTNAGC 2 cut(s) 1878, 2724
Bpu14I TTCGAA 2 cut(s) 153, 2420
BpuEI CTTGAG 1 cut(s) 2919
BpuMI CCSGG 1 cut(s) 2812
Bsa29I ATCGAT 1 cut(s) 2355
BsaAI YACGTR 1 cut(s) 2441
BsaBI GATNNNNATC 2 cut(s) 336, 2351
BsaI GGTCTC 2 cut(s) 1448, 2832
BsaJI CCNNGG 8 cut(s) 677, 1036, 1164, 1447, 1820, 2046, 2594, 2811
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 5 cut(s) 872, 2196, 2309, 2444, 2644
Bse1I ACTGG 7 cut(s) 91, 672, 1083, 1520, 1724, 1939, 2178
Bse21I CCTNAGG 1 cut(s) 2000
Bse3DI GCAATG 5 cut(s) 1104, 1389, 1402, 1614, 2410
Bse8I GATNNNNATC 2 cut(s) 336, 2351
BseBI CCWGG 3 cut(s) 624, 2047, 2595
BseCI ATCGAT 1 cut(s) 2355
BseDI CCNNGG 8 cut(s) 677, 1036, 1164, 1447, 1820, 2046, 2594, 2811
BseGI GGATG 8 cut(s) 823, 862, 967, 1066, 1600, 1745, 2653, 2821
BseJI GATNNNNATC 2 cut(s) 336, 2351
BseLI CCNNNNNNNGG 5 cut(s) 872, 2196, 2309, 2444, 2644
BseMI GCAATG 5 cut(s) 1104, 1389, 1402, 1614, 2410
BseMII CTCAG 2 cut(s) 423, 2715
BseNI ACTGG 7 cut(s) 91, 672, 1083, 1520, 1724, 1939, 2178
BseRI GAGGAG 3 cut(s) 1587, 1868, 2778
BseXI GCAGC 9 cut(s) 77, 517, 709, 795, 817, 1711, 2191, 2521, 2691
BseYI CCCAGC 1 cut(s) 1899
BshFI GGCC 4 cut(s) 19, 1585, 2810, 2916
BshNI GGYRCC 1 cut(s) 2174
BshVI ATCGAT 1 cut(s) 2355
BsiHKAI GWGCWC 2 cut(s) 375, 2149
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 1 cut(s) 2811
BslFI GGGAC 5 cut(s) 179, 1145, 1328, 2399, 2564
BslI CCNNNNNNNGG 5 cut(s) 872, 2196, 2309, 2444, 2644
BsmAI GTCTC 4 cut(s) 399, 658, 1448, 2832
BsmFI GGGAC 5 cut(s) 179, 1145, 1328, 2399, 2564
BsmI GAATGC 3 cut(s) 357, 2047, 2707
BsnI GGCC 4 cut(s) 19, 1585, 2810, 2916
Bso31I GGTCTC 2 cut(s) 1448, 2832
BsoBI CYCGRG 1 cut(s) 483
Bsp119I TTCGAA 2 cut(s) 153, 2420
Bsp1286I GDGCHC 2 cut(s) 375, 2149
Bsp1407I TGTACA 1 cut(s) 1005
Bsp1720I GCTNAGC 2 cut(s) 1878, 2724
Bsp19I CCATGG 1 cut(s) 1036
BspACI CCGC 3 cut(s) 973, 2429, 2615
BspANI GGCC 4 cut(s) 19, 1585, 2810, 2916
BspCNI CTCAG 2 cut(s) 424, 2716
BspDI ATCGAT 1 cut(s) 2355
BspLI GGNNCC 5 cut(s) 628, 1080, 1230, 1264, 2176
BspMAI CTGCAG 1 cut(s) 694
BspMI ACCTGC 2 cut(s) 588, 2580
BspPI GGATC 9 cut(s) 50, 577, 1223, 1236, 1257, 1270, 1331, 1759, 2739
BspT104I TTCGAA 2 cut(s) 153, 2420
BspT107I GGYRCC 1 cut(s) 2174
BspTNI GGTCTC 2 cut(s) 1448, 2832
BsrDI GCAATG 5 cut(s) 1104, 1389, 1402, 1614, 2410
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 7 cut(s) 91, 672, 1083, 1520, 1724, 1939, 2178
BssECI CCNNGG 8 cut(s) 677, 1036, 1164, 1447, 1820, 2046, 2594, 2811
BssT1I CCWWGG 4 cut(s) 677, 1036, 1164, 1447
Bst2UI CCWGG 3 cut(s) 624, 2047, 2595
Bst4CI ACNGT 9 cut(s) 218, 505, 545, 758, 1109, 2080, 2309, 2378, 2822
Bst6I CTCTTC 1 cut(s) 2491
BstAUI TGTACA 1 cut(s) 1005
BstBAI YACGTR 1 cut(s) 2441
BstBI TTCGAA 2 cut(s) 153, 2420
BstC8I GCNNGC 4 cut(s) 1147, 2145, 2202, 2808
BstDEI CTNAG 5 cut(s) 432, 801, 1878, 2000, 2724
BstDSI CCRYGG 2 cut(s) 1036, 1820
BstEII GGTNACC 1 cut(s) 2567
BstF5I GGATG 8 cut(s) 823, 862, 967, 1066, 1600, 1745, 2653, 2821
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstMAI GTCTC 4 cut(s) 399, 658, 1448, 2832
BstMWI GCNNNNNNNGC 3 cut(s) 176, 558, 2803
BstNI CCWGG 3 cut(s) 624, 2047, 2595
BstNSI RCATGY 6 cut(s) 1006, 1061, 1149, 1382, 1899, 2579
BstPI GGTNACC 1 cut(s) 2567
BstSCI CCNGG 4 cut(s) 622, 2045, 2593, 2810
BstSFI CTRYAG 5 cut(s) 94, 198, 522, 690, 2945
BstV1I GCAGC 9 cut(s) 77, 517, 709, 795, 817, 1711, 2191, 2521, 2691
BstV2I GAAGAC 3 cut(s) 884, 1796, 2337
BstX2I RGATCY 6 cut(s) 569, 1228, 1262, 1336, 1751, 2752
BstXI CCANNNNNNTGG 2 cut(s) 684, 2139
BstYI RGATCY 6 cut(s) 569, 1228, 1262, 1336, 1751, 2752
Bsu15I ATCGAT 1 cut(s) 2355
Bsu36I CCTNAGG 1 cut(s) 2000
BsuRI GGCC 4 cut(s) 19, 1585, 2810, 2916
BsuTUI ATCGAT 1 cut(s) 2355
BtgI CCRYGG 2 cut(s) 1036, 1820
BtgZI GCGATG 1 cut(s) 327
BtrI CACGTC 2 cut(s) 869, 1352
BtsCI GGATG 8 cut(s) 823, 862, 967, 1066, 1600, 1745, 2653, 2821
BtsIMutI CAGTG 5 cut(s) 243, 501, 550, 1513, 2314
BveI ACCTGC 2 cut(s) 588, 2580
Cac8I GCNNGC 4 cut(s) 1147, 2145, 2202, 2808
CfoI GCGC 1 cut(s) 676
Cfr13I GGNCC 3 cut(s) 1161, 1187, 2501
ClaI ATCGAT 1 cut(s) 2355
Csp6I GTAC 7 cut(s) 284, 556, 1006, 1506, 1630, 2076, 2491
CviQI GTAC 7 cut(s) 284, 556, 1006, 1506, 1630, 2076, 2491
DdeI CTNAG 5 cut(s) 432, 801, 1878, 2000, 2724
DraI TTTAAA 1 cut(s) 1557
DrdI GACNNNNNNGTC 2 cut(s) 2393, 2576
DriI GACNNNNNGTC 1 cut(s) 2824
DseDI GACNNNNNNGTC 2 cut(s) 2393, 2576
EaeI YGGCCR 2 cut(s) 2808, 2914
Eam1104I CTCTTC 1 cut(s) 2491
Eam1105I GACNNNNNGTC 1 cut(s) 2824
EarI CTCTTC 1 cut(s) 2491
EciI GGCGGA 2 cut(s) 2418, 2630
Eco130I CCWWGG 4 cut(s) 677, 1036, 1164, 1447
Eco31I GGTCTC 2 cut(s) 1448, 2832
Eco32I GATATC 1 cut(s) 2923
Eco47I GGWCC 3 cut(s) 1161, 1187, 2501
Eco57I CTGAAG 3 cut(s) 138, 400, 1188
Eco72I CACGTG 1 cut(s) 2441
Eco81I CCTNAGG 1 cut(s) 2000
Eco88I CYCGRG 1 cut(s) 483
Eco91I GGTNACC 1 cut(s) 2567
EcoO65I GGTNACC 1 cut(s) 2567
EcoRI GAATTC 1 cut(s) 1712
EcoRII CCWGG 3 cut(s) 622, 2045, 2593
EcoRV GATATC 1 cut(s) 2923
EcoT14I CCWWGG 4 cut(s) 677, 1036, 1164, 1447
EcoT22I ATGCAT 3 cut(s) 1147, 1151, 1614
ErhI CCWWGG 4 cut(s) 677, 1036, 1164, 1447
FaqI GGGAC 5 cut(s) 179, 1145, 1328, 2399, 2564
FauI CCCGC 1 cut(s) 980
FauNDI CATATG 1 cut(s) 1522
FbaI TGATCA 1 cut(s) 1474
FblI GTMKAC 2 cut(s) 220, 1277
Fnu4HI GCNGC 9 cut(s) 91, 531, 698, 806, 809, 1725, 2205, 2535, 2680
FokI GGATG 8 cut(s) 810, 849, 954, 1053, 1587, 1752, 2660, 2828
Fsp4HI GCNGC 9 cut(s) 91, 531, 698, 806, 809, 1725, 2205, 2535, 2680
FspBI CTAG 2 cut(s) 287, 635
GlaI GCGC 1 cut(s) 675
GluI GCNGC 9 cut(s) 91, 531, 698, 806, 809, 1725, 2205, 2535, 2680
GsaI CCCAGC 1 cut(s) 1903
HaeII RGCGCY 1 cut(s) 677
HaeIII GGCC 4 cut(s) 19, 1585, 2810, 2916
HapII CCGG 1 cut(s) 2811
HhaI GCGC 1 cut(s) 676
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 1 cut(s) 221
HindII GTYRAC 1 cut(s) 221
HindIII AAGCTT 2 cut(s) 608, 1880
HpaII CCGG 1 cut(s) 2811
HphI GGTGA 5 cut(s) 612, 1541, 1550, 2579, 2641
Hpy166II GTNNAC 7 cut(s) 76, 221, 754, 847, 1278, 1630, 1745
Hpy188I TCNGA 8 cut(s) 271, 419, 828, 1071, 1569, 2274, 2411, 2762
Hpy8I GTNNAC 7 cut(s) 76, 221, 754, 847, 1278, 1630, 1745
HpyCH4III ACNGT 9 cut(s) 218, 505, 545, 758, 1109, 2080, 2309, 2378, 2822
HpyCH4IV ACGT 5 cut(s) 868, 1351, 2388, 2440, 2619
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 558, 2803
HpyF3I CTNAG 5 cut(s) 432, 801, 1878, 2000, 2724
HpySE526I ACGT 5 cut(s) 868, 1351, 2388, 2440, 2619
HspAI GCGC 1 cut(s) 674
Ksp22I TGATCA 1 cut(s) 1474
LmnI GCTCC 4 cut(s) 309, 378, 632, 1822
Lsp1109I GCAGC 9 cut(s) 77, 517, 709, 795, 817, 1711, 2191, 2521, 2691
LweI GCATC 4 cut(s) 832, 868, 1075, 1978
MaeI CTAG 2 cut(s) 287, 635
MaeII ACGT 5 cut(s) 868, 1351, 2388, 2440, 2619
MaeIII GTNAC 7 cut(s) 989, 1529, 1672, 2261, 2567, 2629, 2708
MfeI CAATTG 1 cut(s) 1425
MflI RGATCY 6 cut(s) 569, 1228, 1262, 1336, 1751, 2752
MhlI GDGCHC 2 cut(s) 375, 2149
MlsI TGGCCA 1 cut(s) 2916
MluNI TGGCCA 1 cut(s) 2916
MlyI GAGTC 4 cut(s) 201, 1184, 1814, 2556
MmeI TCCRAC 3 cut(s) 1094, 1993, 2524
Mox20I TGGCCA 1 cut(s) 2916
Mph1103I ATGCAT 3 cut(s) 1147, 1151, 1614
MroXI GAANNNNTTC 2 cut(s) 1962, 2103
MscI TGGCCA 1 cut(s) 2916
MslI CAYNNNNRTG 7 cut(s) 366, 1007, 1301, 1689, 1734, 2509, 2696
Msp20I TGGCCA 1 cut(s) 2916
MspA1I CMGCKG 4 cut(s) 808, 975, 1208, 2723
MspI CCGG 1 cut(s) 2811
MspR9I CCNGG 4 cut(s) 624, 2047, 2595, 2812
MunI CAATTG 1 cut(s) 1425
Mva1269I GAATGC 3 cut(s) 357, 2047, 2707
MvaI CCWGG 3 cut(s) 624, 2047, 2595
MwoI GCNNNNNNNGC 3 cut(s) 176, 558, 2803
NciI CCSGG 1 cut(s) 2812
NcoI CCATGG 1 cut(s) 1036
NdeI CATATG 1 cut(s) 1522
NlaIV GGNNCC 5 cut(s) 628, 1080, 1230, 1264, 2176
NmuCI GTSAC 5 cut(s) 989, 1529, 2261, 2567, 2629
NsiI ATGCAT 3 cut(s) 1147, 1151, 1614
NspI RCATGY 6 cut(s) 1006, 1061, 1149, 1382, 1899, 2579
NspV TTCGAA 2 cut(s) 153, 2420
PaeI GCATGC 1 cut(s) 1149
PciI ACATGT 1 cut(s) 1057
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 2394
PctI GAATGC 3 cut(s) 357, 2047, 2707
PdmI GAANNNNTTC 2 cut(s) 1962, 2103
PfeI GAWTC 6 cut(s) 164, 639, 703, 761, 890, 2658
PflMI CCANNNNNTGG 1 cut(s) 2309
PkrI GCNGC 9 cut(s) 92, 532, 699, 807, 810, 1726, 2206, 2536, 2681
PleI GAGTC 4 cut(s) 200, 1183, 1813, 2555
PmaCI CACGTG 1 cut(s) 2441
PmlI CACGTG 1 cut(s) 2441
PpsI GAGTC 4 cut(s) 200, 1183, 1813, 2555
Ppu21I YACGTR 1 cut(s) 2441
PscI ACATGT 1 cut(s) 1057
PshBI ATTAAT 1 cut(s) 186
Psp6I CCWGG 3 cut(s) 622, 2045, 2593
PspCI CACGTG 1 cut(s) 2441
PspEI GGTNACC 1 cut(s) 2567
PspFI CCCAGC 1 cut(s) 1899
PspGI CCWGG 3 cut(s) 622, 2045, 2593
PspN4I GGNNCC 5 cut(s) 628, 1080, 1230, 1264, 2176
PspPI GGNCC 3 cut(s) 1161, 1187, 2501
PsrI GAACNNNNNNTAC 4 cut(s) 276, 308, 1261, 1293
PstI CTGCAG 1 cut(s) 694
PsuI RGATCY 6 cut(s) 569, 1228, 1262, 1336, 1751, 2752
PvuII CAGCTG 3 cut(s) 808, 1208, 2723
RsaI GTAC 7 cut(s) 285, 557, 1007, 1507, 1631, 2077, 2492
RsaNI GTAC 7 cut(s) 284, 556, 1006, 1506, 1630, 2076, 2491
RseI CAYNNNNRTG 7 cut(s) 366, 1007, 1301, 1689, 1734, 2509, 2696
SalI GTCGAC 1 cut(s) 219
SatI GCNGC 9 cut(s) 91, 531, 698, 806, 809, 1725, 2205, 2535, 2680
Sau96I GGNCC 3 cut(s) 1161, 1187, 2501
SchI GAGTC 4 cut(s) 201, 1184, 1814, 2556
ScrFI CCNGG 4 cut(s) 624, 2047, 2595, 2812
SduI GDGCHC 2 cut(s) 375, 2149
SfaNI GCATC 4 cut(s) 832, 868, 1075, 1978
SfcI CTRYAG 5 cut(s) 94, 198, 522, 690, 2945
SfuI TTCGAA 2 cut(s) 153, 2420
SinI GGWCC 3 cut(s) 1161, 1187, 2501
SmiMI CAYNNNNRTG 7 cut(s) 366, 1007, 1301, 1689, 1734, 2509, 2696
SmlI CTYRAG 1 cut(s) 2934
SmoI CTYRAG 1 cut(s) 2934
SphI GCATGC 1 cut(s) 1149
SsiI CCGC 3 cut(s) 973, 2429, 2615
SspI AATATT 3 cut(s) 1027, 1362, 1415
SspMI CTAG 2 cut(s) 287, 635
StyD4I CCNGG 4 cut(s) 622, 2045, 2593, 2810
StyI CCWWGG 4 cut(s) 677, 1036, 1164, 1447
TaaI ACNGT 9 cut(s) 218, 505, 545, 758, 1109, 2080, 2309, 2378, 2822
TaiI ACGT 5 cut(s) 871, 1354, 2391, 2443, 2622
TatI WGTACW 2 cut(s) 283, 1005
TfiI GAWTC 6 cut(s) 164, 639, 703, 761, 890, 2658
TscAI CASTG 5 cut(s) 250, 508, 550, 1520, 2314
TseFI GTSAC 5 cut(s) 989, 1529, 2261, 2567, 2629
TseI GCWGC 9 cut(s) 90, 530, 697, 805, 808, 1724, 2204, 2534, 2679
Tsp45I GTSAC 5 cut(s) 989, 1529, 2261, 2567, 2629
TspGWI ACGGA 4 cut(s) 21, 632, 1809, 2508
TspRI CASTG 5 cut(s) 250, 508, 550, 1520, 2314
Van91I CCANNNNNTGG 1 cut(s) 2309
VpaK11BI GGWCC 3 cut(s) 1161, 1187, 2501
VspI ATTAAT 1 cut(s) 186
XapI RAATTY 5 cut(s) 233, 1712, 1759, 1958, 2416
XceI RCATGY 6 cut(s) 1006, 1061, 1149, 1382, 1899, 2579
XcmI CCANNNNNNNNNTGG 2 cut(s) 2645, 2725
XmiI GTMKAC 2 cut(s) 220, 1277
XmnI GAANNNNTTC 2 cut(s) 1962, 2103
XspI CTAG 2 cut(s) 287, 635
Zsp2I ATGCAT 3 cut(s) 1147, 1151, 1614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.