Rroxscaffold_4G00325740

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010440
Physical Location & Seq
Reverse (-)
57586601 .. 57600886
14286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325740.1

Sequence Viewer

Length: 3183 bp
ATGGCTTCTTCTATGATGGGTCAACTGGTTTCTCCCTTGGAGAATGGACATCATGTGTGGGAGGACCAATCTTTCATCAAGTGGAATAAGAGAGATGCACATGTCACTTTACGTTGCCATGAATCAATTGAAGGATCTCTTAAATACTGGTATGAACGCAACAAAGTGAATTTTATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTTCCTGAAGCTCTTGATAGTGCAGCTCTTTGGATCAAGGGCTTGCCTTTTGTGCAGTCTTTGTCAGGCTATTGGAAATTTTACTTAGCTTCCACTCCTGGAAATGTTCCTTTGAATTTTTACCAGACCTCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGGTGTAATTGAAGCTTATGTAATTATTAAAATGGTTTCACAAGTGCAGATGCATGGATTTGATCGGCCTATATATACTAATTTTGAATATCCATTTCCTCTTGATCCACCTTTTGTTCATGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTCATCCCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATTACTGATTATTGTTATCCTTGTGGGACGGACAAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGTGATGGCTCTTACCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTTCTTGCCAAGCCACAGGTTGAAGTGAAAATAGATAACTCCCAAGAAACATCCAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAGCTGGTACAGCAGTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCACCAAGGACAGTTCTTGGATTTCGTGATTATTTGGTGGTAGGGAGACTGGAACTGCCAAGGCTTTGGTCTGCAGAGCAGCCGAATCTGTACACTCTGGTTGTGATATTGAAAGATACATCTGGCAATATTGTGGACTGTGAATCATGCCTAGTAGGCATACGACAAGTATCAAAAGCTCCTAAGCAGCTGCTTGTTAATGGGCATCCAATAACAATAAGGGGTGTGAACAGGCATGAGCACCATCCACGTCTGGGGAAGACAAATATAGAATCGTGCATGGTTAAGGATTTGGTTTTAATGAAGCAATACAATATCAATGCTGTAAGAAACAGCCATTATCCTCAACATCCCCGCTGGTATGAGCTTTGCGACATGTTTGGCATGTACATGATAGATGAAGCCAATATTGAAACCCATGGTTTCGATTATACTGGACATGTGAAACATCCGACTTTGGAACCCAGTTGGGCAACTGCAATGTTGGACCGTGTGATGGGCATGGTTGAGAGGGACAAAAATCATGCGTGCATTATCTCTTGGTATTCACATGCAATGGGAAATAGCAATGGAAATATACATGAATATTGGGAAGCAATTGATGGCACATTTGGTCTCCAAGGAGGCTTTATCTGGGAGTGGGTTGATCAGGGCTTACTGAAAGATAGTGCTGATGGTACTAAACACTGGGCATATGGTGGTGACTTTGGTGACATTCCTAATGATTTAAACTTCTGTTTGAATGGCCTTGTGTGGCCCGATCGAACTCCTCATCCTGTAATGCATGGAGCTGAATTTCCCTTTGCAGATAACAAATACTCACTTTTACGAAACAACAAAAAGCATTGGAATTCAGCTGGGCTGCTCATGGAGATGGATGTGAACTTGGATCTGGAAATTTATCCCTTCCCCTTGATTGAACCCCAGAAGACTTTTCATATTGAGTTGCAGTCAGCTCCGTGGCATACTCTATGGGCTTCATCATCAGCAGAGGAGTTTTTCTTAACAATAAGTGCTAAGCTTTTGCATGCCACATGCTGGGTTGAAGCTGGTCATGTCATCTCATCTACACAAAGTATATCAATGGTTGGGAAAACTGAAGCTAGATTATTTGATATTTCTGAGAGGCATATGAATATGTGGACTGGGGCTCTTCAGTCTAGTATCGGGAAGCAAAAATGGTGGGATATGTATGGAGAAGTGGCAGAAAGGATTGAAAATCTTATGTTGGAAGTAGTGGGTAAAGGAAAGATGGATCTCTTGGTTAGTTGGAAAGATTTATCTCGTCCACAGAATGTAGGTGTTCAGAGGTTTGCGTCAAACCCCATAGATAAATTCTCAGGACACTTATTTGATCCCGTTGATTCACTGCAACAGTCTGGACTTGACAGGAGTCTTTGTTATGCTGAATTATTGGATAATGACAAAGGCGGAGGTGCCAGTAGTTATTCGTCCAAGTGGCAAGCTGCTCATATTGATAACCTTCATTATATTACCAAGAGCTGTTCTGTACAGAATATGACTGACCATCTTGTGAAAGTAGCTGTTGTTTTTCTCGGTGTTCCAAACAGTGGGGACGGTTCTGGGGTGGAAGACACAAATGCCTTAATTGAAATTGATGTGATTTACACAATCTACAGTTCAGGGGACGTTGTTGTTGAATGCAATGTAAGACCAAGTTCAAACCTTCCGCCTCTGCCACGTGTGGGAGTTGAGTTCCACTTGAAGAAGTCAATAGACCAGATTAAATGGTACGGAAGAGGACCATTTGAATGTTATCCAGACAGAAAAGCAGCTGCTCATGTTGGAGTCTATGAGCAGAAAGTGAGTGATCTGCATGTCCCTTATATAGTTCCTGGGGAGTGTTCAGGTAGGGCAGACGTTAGGTGGGTTACATTTCAAAACAAGGATGGTTTGGGAATCTATGCTTCAATATATGGCAGCTCTCCACCCATGCAAATAAATGCAAGTTATTACACCACAGCTGAGCTGGATCGGGCAACACACAATGAAGATCTTATCAGAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTTGCTGGGGATGATAGTTGGTCTCCATGTGTCCATGACAAGTATCTGATTCCTCCTGTTCCAAGCTCATTCTCAATTAGGCTCGCTCCAGTAACTGCTGCAACCTCTGGCCATGATATCTACAAATCTCAACTTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1060

Amino Acids

119.68

Weight (kDa)

5.51

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 270 2.1e-46 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 272 - 379 1.4e-08 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 386 - 505 2.1e-44 Glycosyl hydrolases family 2, TIM barrel domain
Glyco_hydro_2_C PF02836 502 - 586 2.9e-20 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 625 - 682 5.3e-07 Beta-galactosidase, domain 4
Bgal_small_N PF02929 795 - 1041 9.4e-61 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2408
AccB7I CCANNNNNTGG 3 cut(s) 543, 2010, 2543
AccI GTMKAC 1 cut(s) 640
AciI CCGC 3 cut(s) 1327, 2403, 2663
AclWI GGATC 8 cut(s) 142, 251, 470, 931, 1869, 2235, 2321, 2973
AcoI YGGCCR 1 cut(s) 3148
AcsI RAATTY 9 cut(s) 169, 287, 325, 558, 653, 1766, 1822, 1869, 2306
AcuI CTGAAG 3 cut(s) 237, 2091, 2111
AcvI CACGTG 1 cut(s) 2675
AfaI GTAC 7 cut(s) 705, 911, 1064, 1361, 1651, 2484, 2726
AfiI CCNNNNNNNGG 7 cut(s) 543, 1226, 1468, 2010, 2543, 2678, 3046
AflIII ACRYGT 4 cut(s) 100, 1347, 1411, 2674
AjiI CACGTC 1 cut(s) 1223
AjnI CCWGG 3 cut(s) 307, 370, 2827
AloI GAACNNNNNNTCC 2 cut(s) 1729, 1761
Alw21I GWGCWC 1 cut(s) 1215
Alw26I GTCTC 3 cut(s) 1012, 1592, 3066
AlwI GGATC 8 cut(s) 142, 251, 470, 931, 1869, 2235, 2321, 2973
AlwNI CAGNNNCTG 1 cut(s) 3134
AoxI GGCC 5 cut(s) 437, 1717, 1727, 3037, 3148
ApoI RAATTY 9 cut(s) 169, 287, 325, 558, 653, 1766, 1822, 1869, 2306
AspS9I GGNCC 5 cut(s) 64, 1459, 1728, 2735, 3037
AsuHPI GGTGA 3 cut(s) 966, 1685, 1694
AsuII TTCGAA 1 cut(s) 573
AvaII GGWCC 3 cut(s) 64, 1459, 2735
BaeI ACNNNNGTAYC 2 cut(s) 901, 934
BalI TGGCCA 1 cut(s) 3150
BanI GGYRCC 1 cut(s) 2408
BanII GRGCYC 1 cut(s) 2125
BbrPI CACGTG 1 cut(s) 2675
BbsI GAAGAC 3 cut(s) 1238, 1907, 2571
Bbv12I GWGCWC 1 cut(s) 1215
BciT130I CCWGG 3 cut(s) 309, 372, 2829
BciVI GTATCC 1 cut(s) 190
BclI TGATCA 2 cut(s) 1618, 3022
BcoDI GTCTC 3 cut(s) 1012, 1592, 3066
BfaI CTAG 4 cut(s) 707, 1124, 2076, 2133
BfmI CTRYAG 6 cut(s) 514, 618, 876, 1044, 2608, 3179
BfuI GTATCC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 1128
BglII AGATCT 1 cut(s) 2986
BlpI GCTNAGC 2 cut(s) 1989, 2958
Bme1390I CCNGG 3 cut(s) 309, 372, 2829
Bme18I GGWCC 3 cut(s) 64, 1459, 2735
BmgBI CACGTC 1 cut(s) 1223
BmgT120I GGNCC 5 cut(s) 64, 1459, 1728, 2735, 3037
BmiI GGNNCC 2 cut(s) 1434, 2410
BmrFI CCNGG 3 cut(s) 309, 372, 2829
BmrI ACTGGG 3 cut(s) 1431, 1669, 2127
BmsI GCATC 4 cut(s) 85, 196, 411, 1186
BmuI ACTGGG 3 cut(s) 1431, 1669, 2127
BoxI GACNNNNGTC 1 cut(s) 2364
BpiI GAAGAC 3 cut(s) 1238, 1907, 2571
BpmI CTGGAG 1 cut(s) 3111
Bpu10I CCTNAGC 1 cut(s) 1155
Bpu1102I GCTNAGC 2 cut(s) 1989, 2958
Bpu14I TTCGAA 1 cut(s) 573
BsaAI YACGTR 1 cut(s) 2675
BsaBI GATNNNNATC 1 cut(s) 756
BsaI GGTCTC 2 cut(s) 1592, 3066
BsaJI CCNNGG 8 cut(s) 36, 194, 977, 1031, 1390, 1591, 1931, 2828
BsaXI ACNNNNNCTCC 4 cut(s) 603, 633, 2356, 2386
Bsc4I CCNNNNNNNGG 7 cut(s) 543, 1226, 1468, 2010, 2543, 2678, 3046
Bse3DI GCAATG 4 cut(s) 1458, 1533, 1546, 2644
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 3 cut(s) 309, 372, 2829
BseDI CCNNGG 8 cut(s) 36, 194, 977, 1031, 1390, 1591, 1931, 2828
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 7 cut(s) 543, 1226, 1468, 2010, 2543, 2678, 3046
BseMI GCAATG 4 cut(s) 1458, 1533, 1546, 2644
BseMII CTCAG 4 cut(s) 345, 2085, 2325, 2949
BseRI GAGGAG 3 cut(s) 1731, 1979, 3012
BseYI CCCAGC 3 cut(s) 1829, 2010, 3044
BsgI GTGCAG 4 cut(s) 174, 252, 284, 437
Bsh1285I CGRYCG 1 cut(s) 1735
BshFI GGCC 5 cut(s) 439, 1719, 1729, 3039, 3150
BshNI GGYRCC 1 cut(s) 2408
BsiEI CGRYCG 1 cut(s) 1735
BsiHKAI GWGCWC 1 cut(s) 1215
BslFI GGGAC 5 cut(s) 700, 1499, 2561, 2633, 2798
BslI CCNNNNNNNGG 7 cut(s) 543, 1226, 1468, 2010, 2543, 2678, 3046
BsmAI GTCTC 3 cut(s) 1012, 1592, 3066
BsmFI GGGAC 5 cut(s) 700, 1499, 2561, 2633, 2798
BsmI GAATGC 2 cut(s) 777, 2639
BsnI GGCC 5 cut(s) 439, 1719, 1729, 3039, 3150
Bso31I GGTCTC 2 cut(s) 1592, 3066
Bsp119I TTCGAA 1 cut(s) 573
Bsp1286I GDGCHC 2 cut(s) 1215, 2125
Bsp1407I TGTACA 3 cut(s) 1062, 1359, 2482
Bsp1720I GCTNAGC 2 cut(s) 1989, 2958
Bsp19I CCATGG 1 cut(s) 1390
BspACI CCGC 3 cut(s) 1327, 2403, 2663
BspANI GGCC 5 cut(s) 439, 1719, 1729, 3039, 3150
BspCNI CTCAG 4 cut(s) 346, 2086, 2324, 2950
BspLI GGNNCC 2 cut(s) 1434, 2410
BspMAI CTGCAG 1 cut(s) 1048
BspPI GGATC 8 cut(s) 142, 251, 470, 931, 1869, 2235, 2321, 2973
BspQI GCTCTTC 1 cut(s) 2130
BspT104I TTCGAA 1 cut(s) 573
BspT107I GGYRCC 1 cut(s) 2408
BspTNI GGTCTC 2 cut(s) 1592, 3066
BsrDI GCAATG 4 cut(s) 1458, 1533, 1546, 2644
BsrGI TGTACA 3 cut(s) 1062, 1359, 2482
BssECI CCNNGG 8 cut(s) 36, 194, 977, 1031, 1390, 1591, 1931, 2828
BssT1I CCWWGG 6 cut(s) 36, 194, 977, 1031, 1390, 1591
Bst2UI CCWGG 3 cut(s) 309, 372, 2829
Bst6I CTCTTC 2 cut(s) 2130, 2725
BstAUI TGTACA 3 cut(s) 1062, 1359, 2482
BstBAI YACGTR 1 cut(s) 2675
BstBI TTCGAA 1 cut(s) 573
BstC8I GCNNGC 5 cut(s) 254, 1501, 2001, 2436, 3123
BstDEI CTNAG 7 cut(s) 295, 354, 1155, 1989, 2094, 2311, 2958
BstDSI CCRYGG 2 cut(s) 1390, 1931
BstMAI GTCTC 3 cut(s) 1012, 1592, 3066
BstMCI CGRYCG 1 cut(s) 1735
BstMWI GCNNNNNNNGC 4 cut(s) 262, 596, 912, 1128
BstNI CCWGG 3 cut(s) 309, 372, 2829
BstNSI RCATGY 8 cut(s) 104, 1351, 1360, 1415, 1526, 2003, 2010, 2813
BstPAI GACNNNNGTC 1 cut(s) 2364
BstSCI CCNGG 3 cut(s) 307, 370, 2827
BstSFI CTRYAG 6 cut(s) 514, 618, 876, 1044, 2608, 3179
BstV2I GAAGAC 3 cut(s) 1238, 1907, 2571
BstX2I RGATCY 5 cut(s) 134, 923, 1861, 2227, 2986
BstXI CCANNNNNNTGG 1 cut(s) 1038
BstYI RGATCY 5 cut(s) 134, 923, 1861, 2227, 2986
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 5 cut(s) 439, 1719, 1729, 3039, 3150
BtgI CCRYGG 2 cut(s) 1390, 1931
BtrI CACGTC 1 cut(s) 1223
BtsI GCAGTG 2 cut(s) 922, 2339
BtsIMutI CAGTG 6 cut(s) 862, 904, 922, 1657, 2339, 2548
Cac8I GCNNGC 5 cut(s) 254, 1501, 2001, 2436, 3123
CaiI CAGNNNCTG 1 cut(s) 3134
Cfr13I GGNCC 5 cut(s) 64, 1459, 1728, 2735, 3037
CseI GACGC 1 cut(s) 2277
Csp6I GTAC 7 cut(s) 704, 910, 1063, 1360, 1650, 2483, 2725
CspCI CAANNNNNGTGG 2 cut(s) 2135, 2170
CviQI GTAC 7 cut(s) 704, 910, 1063, 1360, 1650, 2483, 2725
DdeI CTNAG 7 cut(s) 295, 354, 1155, 1989, 2094, 2311, 2958
DraI TTTAAA 1 cut(s) 1701
EaeI YGGCCR 1 cut(s) 3148
Eam1104I CTCTTC 2 cut(s) 2130, 2725
EarI CTCTTC 2 cut(s) 2130, 2725
EciI GGCGGA 2 cut(s) 2418, 2652
Eco130I CCWWGG 6 cut(s) 36, 194, 977, 1031, 1390, 1591
Eco24I GRGCYC 1 cut(s) 2125
Eco31I GGTCTC 2 cut(s) 1592, 3066
Eco32I GATATC 1 cut(s) 3157
Eco47I GGWCC 3 cut(s) 64, 1459, 2735
Eco57I CTGAAG 3 cut(s) 237, 2091, 2111
Eco72I CACGTG 1 cut(s) 2675
EcoRI GAATTC 1 cut(s) 1822
EcoRII CCWGG 3 cut(s) 307, 370, 2827
EcoRV GATATC 1 cut(s) 3157
EcoT14I CCWWGG 6 cut(s) 36, 194, 977, 1031, 1390, 1591
EcoT22I ATGCAT 2 cut(s) 426, 1758
EcoT38I GRGCYC 1 cut(s) 2125
ErhI CCWWGG 6 cut(s) 36, 194, 977, 1031, 1390, 1591
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 5 cut(s) 700, 1499, 2561, 2633, 2798
FauI CCCGC 1 cut(s) 1334
FauNDI CATATG 2 cut(s) 1666, 2103
FbaI TGATCA 2 cut(s) 1618, 3022
FblI GTMKAC 1 cut(s) 640
FriOI GRGCYC 1 cut(s) 2125
FspBI CTAG 4 cut(s) 707, 1124, 2076, 2133
GsaI CCCAGC 3 cut(s) 1833, 2014, 3048
GsuI CTGGAG 1 cut(s) 3111
HaeIII GGCC 5 cut(s) 439, 1719, 1729, 3039, 3150
HgaI GACGC 1 cut(s) 2277
HincII GTYRAC 2 cut(s) 23, 641
HindII GTYRAC 2 cut(s) 23, 641
HindIII AAGCTT 3 cut(s) 384, 962, 1991
HphI GGTGA 3 cut(s) 966, 1685, 1694
Hpy166II GTNNAC 9 cut(s) 23, 496, 641, 1065, 1108, 1201, 1855, 2115, 2261
Hpy188I TCNGA 6 cut(s) 355, 1425, 2095, 2280, 2996, 3087
Hpy8I GTNNAC 9 cut(s) 23, 496, 641, 1065, 1108, 1201, 1855, 2115, 2261
HpyAV CCTTC 6 cut(s) 125, 541, 601, 1888, 2465, 2669
HpyCH4IV ACGT 5 cut(s) 112, 1222, 2622, 2674, 2853
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 596, 912, 1128
HpyF3I CTNAG 7 cut(s) 295, 354, 1155, 1989, 2094, 2311, 2958
HpySE526I ACGT 5 cut(s) 112, 1222, 2622, 2674, 2853
Ksp22I TGATCA 2 cut(s) 1618, 3022
LguI GCTCTTC 1 cut(s) 2130
LmnI GCTCC 5 cut(s) 903, 1156, 1760, 1933, 3130
LweI GCATC 4 cut(s) 85, 196, 411, 1186
MaeI CTAG 4 cut(s) 707, 1124, 2076, 2133
MaeII ACGT 5 cut(s) 112, 1222, 2622, 2674, 2853
MaeIII GTNAC 5 cut(s) 103, 1673, 1682, 2863, 3130
MboII GAAGA 9 cut(s) 761, 788, 1243, 1912, 2117, 2576, 2710, 2742, 2996
MfeI CAATTG 2 cut(s) 126, 1569
MflI RGATCY 5 cut(s) 134, 923, 1861, 2227, 2986
MhlI GDGCHC 2 cut(s) 1215, 2125
MlsI TGGCCA 1 cut(s) 3150
MluNI TGGCCA 1 cut(s) 3150
MlyI GAGTC 2 cut(s) 2374, 2790
MmeI TCCRAC 6 cut(s) 207, 1437, 1448, 2181, 2222, 2758
Mox20I TGGCCA 1 cut(s) 3150
Mph1103I ATGCAT 2 cut(s) 426, 1758
MscI TGGCCA 1 cut(s) 3150
MseI TTAA 9 cut(s) 141, 399, 1170, 1257, 1271, 1700, 1976, 2579, 2718
MslI CAYNNNNRTG 4 cut(s) 786, 1361, 1844, 2743
Msp20I TGGCCA 1 cut(s) 3150
MspA1I CMGCKG 5 cut(s) 1162, 1329, 1829, 2768, 2957
MspR9I CCNGG 3 cut(s) 309, 372, 2829
MunI CAATTG 2 cut(s) 126, 1569
Mva1269I GAATGC 2 cut(s) 777, 2639
MvaI CCWGG 3 cut(s) 309, 372, 2829
MwoI GCNNNNNNNGC 4 cut(s) 262, 596, 912, 1128
NcoI CCATGG 1 cut(s) 1390
NdeI CATATG 2 cut(s) 1666, 2103
NlaIV GGNNCC 2 cut(s) 1434, 2410
NmuCI GTSAC 3 cut(s) 103, 1673, 1682
NsiI ATGCAT 2 cut(s) 426, 1758
NspI RCATGY 8 cut(s) 104, 1351, 1360, 1415, 1526, 2003, 2010, 2813
NspV TTCGAA 1 cut(s) 573
PaeI GCATGC 1 cut(s) 2003
PciI ACATGT 3 cut(s) 100, 1347, 1411
PciSI GCTCTTC 1 cut(s) 2130
PcsI WCGNNNNNNNCGW 1 cut(s) 579
PctI GAATGC 2 cut(s) 777, 2639
PfeI GAWTC 9 cut(s) 122, 350, 584, 1057, 1115, 1244, 2336, 2892, 3088
PflMI CCANNNNNTGG 3 cut(s) 543, 2010, 2543
PfoI TCCNGGA 1 cut(s) 307
Ple19I CGATCG 1 cut(s) 1735
PleI GAGTC 2 cut(s) 2373, 2789
PmaCI CACGTG 1 cut(s) 2675
PmlI CACGTG 1 cut(s) 2675
PpsI GAGTC 2 cut(s) 2373, 2789
Ppu21I YACGTR 1 cut(s) 2675
PscI ACATGT 3 cut(s) 100, 1347, 1411
PshAI GACNNNNGTC 1 cut(s) 2364
Psp6I CCWGG 3 cut(s) 307, 370, 2827
PspCI CACGTG 1 cut(s) 2675
PspFI CCCAGC 3 cut(s) 1829, 2010, 3044
PspGI CCWGG 3 cut(s) 307, 370, 2827
PspN4I GGNNCC 2 cut(s) 1434, 2410
PspPI GGNCC 5 cut(s) 64, 1459, 1728, 2735, 3037
PsrI GAACNNNNNNTAC 2 cut(s) 696, 728
PstI CTGCAG 1 cut(s) 1048
PstNI CAGNNNCTG 1 cut(s) 3134
PsuI RGATCY 5 cut(s) 134, 923, 1861, 2227, 2986
PvuI CGATCG 1 cut(s) 1735
PvuII CAGCTG 4 cut(s) 1162, 1829, 2768, 2957
RsaI GTAC 7 cut(s) 705, 911, 1064, 1361, 1651, 2484, 2726
RsaNI GTAC 7 cut(s) 704, 910, 1063, 1360, 1650, 2483, 2725
RseI CAYNNNNRTG 4 cut(s) 786, 1361, 1844, 2743
SalI GTCGAC 1 cut(s) 639
SapI GCTCTTC 1 cut(s) 2130
SaqAI TTAA 9 cut(s) 141, 399, 1170, 1257, 1271, 1700, 1976, 2579, 2718
Sau96I GGNCC 5 cut(s) 64, 1459, 1728, 2735, 3037
SchI GAGTC 2 cut(s) 2374, 2790
ScrFI CCNGG 3 cut(s) 309, 372, 2829
SduI GDGCHC 2 cut(s) 1215, 2125
SfaNI GCATC 4 cut(s) 85, 196, 411, 1186
SfcI CTRYAG 6 cut(s) 514, 618, 876, 1044, 2608, 3179
SfuI TTCGAA 1 cut(s) 573
SinI GGWCC 3 cut(s) 64, 1459, 2735
SmiMI CAYNNNNRTG 4 cut(s) 786, 1361, 1844, 2743
SphI GCATGC 1 cut(s) 2003
SsiI CCGC 3 cut(s) 1327, 2403, 2663
SspI AATATT 3 cut(s) 1102, 1381, 1559
SspMI CTAG 4 cut(s) 707, 1124, 2076, 2133
StyD4I CCNGG 3 cut(s) 307, 370, 2827
StyI CCWWGG 6 cut(s) 36, 194, 977, 1031, 1390, 1591
TaiI ACGT 5 cut(s) 115, 1225, 2625, 2677, 2856
TaqI TCGA 5 cut(s) 573, 582, 640, 1398, 1735
TatI WGTACW 4 cut(s) 703, 1062, 1359, 2482
TfiI GAWTC 9 cut(s) 122, 350, 584, 1057, 1115, 1244, 2336, 2892, 3088
Tru1I TTAA 9 cut(s) 141, 399, 1170, 1257, 1271, 1700, 1976, 2579, 2718
Tru9I TTAA 9 cut(s) 141, 399, 1170, 1257, 1271, 1700, 1976, 2579, 2718
TscAI CASTG 6 cut(s) 862, 904, 922, 1664, 2346, 2548
TseFI GTSAC 3 cut(s) 103, 1673, 1682
Tsp45I GTSAC 3 cut(s) 103, 1673, 1682
TspGWI ACGGA 3 cut(s) 704, 1920, 2742
TspRI CASTG 6 cut(s) 862, 904, 922, 1664, 2346, 2548
Van91I CCANNNNNTGG 3 cut(s) 543, 2010, 2543
VpaK11BI GGWCC 3 cut(s) 64, 1459, 2735
XapI RAATTY 9 cut(s) 169, 287, 325, 558, 653, 1766, 1822, 1869, 2306
XceI RCATGY 8 cut(s) 104, 1351, 1360, 1415, 1526, 2003, 2010, 2813
XcmI CCANNNNNNNNNTGG 1 cut(s) 2959
XmiI GTMKAC 1 cut(s) 640
XspI CTAG 4 cut(s) 707, 1124, 2076, 2133
Zsp2I ATGCAT 2 cut(s) 426, 1758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.