RLG00000030210

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010440
Physical Location & Seq
Reverse (-)
57582856 .. 57596223
13368 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030210

Sequence Viewer

Length: 3324 bp
ATGGCTTCTTCTATGATGGGTCAACTGGTTTCTCCCTTAGAGAATGGACATCATGTGTGGGAGGACCAATCTTTCATCAAGTGGAAGAAGAGAGATGCACATGTCACTTTACGTTGCCATGAATCAATTGAAGGATCTCTTAAATACTGGTATGAACGCAACAAAGTGAATTTTATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTGCCTGAAGCTCTTACTAGTGCAGCTCTTTGGGTCAAGGGCTTGCCTTTTGTGGAGTCTTTGTCAGGCTATTGGAAATTTTACTTAGCTTCCACTCCTGGAAATGTTCCTTTGAATTTTTACCACACTGCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGTTCCTTCAAATTGGCAGCTGCACGGATTTGATCGGCCTATATATACTAATGTGGAATATCCATTTCCTCTTGATCCACCTTTTGTTCATGTGGACAACCCTACTGGCTGCTATAGGACAAATTTTGTCATCCCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATTACTGATTATTGTTATCCTTGTGGGACGGACAAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGTGATGGCTCTTACCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTTCTTTCCAAGCCACAGGTATTTATTGCAGACTATTTCTTCAAATCGAATCTGGCTGAGGATTTCTCTTATGCAGACCTACAGGTTGAAGTGAAAATAGATAACTCCCAAGAAACATCCAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACTGTGGGAGCTGGTACAGCAGTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCACGATTTCGTGATTATTTGCTGGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGCCGAATCTGTACACTCTGGTTGTGATATTGAAAGATACATCTGGCAATATTGTGGACTGTGAATCATGCCTGGTAGGCATACGACAAGTATCAAAAGCCCCTAAGCAGCTGCTTGTTAATGGGCATCCAATAACAATAAGGGGTGTGAACAGGCATGAGCATCATCCACGTCTGGGGAAGACAAATATAGAATCGTGCATGGTTAAGGATTTGGTTTTAATGAAGCAATACAATATCAATGCTGTAAGAAACAGCCATTATCCTCAACATCCCCGCTGGTATGAGCTTTGCGACATGTTTGGCATGTACATGATAGATGAAGCCAATATTGAAACCCATGGTTTCGATTATACTGGACATGTGAAACATCCGACTTTGGAACCCAGTTGGGCAACTGCAATGTTGGACCGTGTGATGGGCATGGTTGAGAGAGACAAAAATCATGCGTGCATTATTTCTTGGTCCTTGGGAAATGAATCTGGATATGGACCTAATCATTCTGCTTTAGCTGGTTGGGTTCGTGGAAAGGATCCCTCACGGCTGTTACATTACGAAGGGGGAGGATCCAGAACCCCGTCTACAGATATTATATGCCCCATGTATATGCGTGTTTGGGACATTGTGAAGATTGCCAAAGATCCGAATGAAACACGTCCTCTAATACTGTGCGAGTATTCACATGCAATGGGAAATAGCAATGGAAATATACATGAATATTGGGAAGCAATTGATGGCACATTTGGTCTCCAAGGAGGCTTTATCTGGGAGTGGGTTGATCAGGGCTTACTGAAAGATAGTGCTGATGGTACTAAACACTGGGCATATGGCGGTGACTTTGGTGACGTTCCTAATGATTTAAACTTCTGTTTGAATGGCCTTGTGTGGCCCGATCGAACTCCTCATCCTGCATTGCATGAAGTGAAGTATGTGTACCAGCCAATCAAGGTTTCATTTAGTGAAGGAACACTAAAGATAACAAATAGTCACTTTTATGAAACAACAAAAGCATTGGAATTCTGCTGGGCTGCTCATGGAGATGGATGTGAACTTGGATCTGGAAATTTATCCCTTCCCTTGATTGAACCCCAGAAGACTTTTCATATTGAGTTGCAGTCAGCTCCGTGGCATACTCTATGGGCTTCATCATCAGCAGAGGAGTTTTTCTTAACAATAAGTGCTAAGCTTTCGCATGCCACATGCTGGGTTGAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTGCCAAAAGAGAATTTGTTCCTCATGTCATCAAGACTAAAGATACTACATTCCTTAAGGAAATTCTTGGGGATACACTTAAAGTCGGCCAACAGAATACCTGGGAGATTATACTGAATCTTAAAATGGGAACAGTTGAAAGCTGGAAGGTGGAAGGAGTTCCTTTGATGACAAAAGGCATATTTCCATGTTTTTGGCGAGCACCCACAGATAATGACAAAGGCGGAGGTGCCGGTAGTTATTCGTCCAAGTGGCAAGCTGCTCATATTGATAACCTTCATTATATTGCCAAGAGCTGTTCTGTACAGAATATGACTGACCATCTTGTGACAGTAGCTGTTGTTTTTCTCGGTGTTCCAAACAGTGGGGACGGTTCTGGGGTTGAAGCCGCAAATGCCTTAATTGAAATTGATGTGATTTACACAATCTACGGTTCAGGGGACGTTGTTGTTGAATGCAATGTAAGACCAAGTTTGAACCTTCCGCCTCTGCCACGTGTGGGAGTTGAGTTCCACTTGGAGAAGTCAGTAGACCAGATTAAATGGTACGGAAGAGGACCATTTGAATGTTATCCAGACAGAAAAGCAGCTGCTCATGTTGGAGTCTATGAGCAGAATGTGAGTGACCTGCATGTCCCTTATATAGTTCCTGGGGAATGTTCAGGAAGGGCAGACGTTAGATGGGTGACATTTCAAAACAAGGATGGTTTGGGAATCTATGCTTCAAAATATGGCAGCTCTCCACCCATGCAAATAAATGCAAGTTACTACACCACAGCTGAGCTGGATCGGGCAACACACAATGAAGATCTTATCAGAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTGGCCGGGGATGATAGTTGGTCTCCATGTGTCCATGACAAGTATCTGATTCCTCCTGTTCCAAGCTCATTCTCAATTAGGCTCTCTCCAATAACTCCTGCAACCTCCGGCCATGATATCTACAAATCTCAACTTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1108

Amino Acids

124.56

Weight (kDa)

5.64

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 260 9.9e-54 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 262 - 386 6.2e-13 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 393 - 673 2.1e-97 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 681 - 774 8.6e-19 Beta-galactosidase, domain 4
Bgal_small_N PF02929 805 - 1088 7.5e-73 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2951
Acc36I ACCTGC 1 cut(s) 2955
AccB1I GGYRCC 1 cut(s) 2549
AccB7I CCANNNNNTGG 3 cut(s) 513, 2274, 2684
AccI GTMKAC 3 cut(s) 610, 1652, 2850
AciI CCGC 5 cut(s) 1348, 1902, 2544, 2709, 2804
AcoI YGGCCR 3 cut(s) 2407, 3183, 3289
AcuI CTGAAG 1 cut(s) 237
AcvI CACGTG 1 cut(s) 2816
AfaI GTAC 8 cut(s) 675, 947, 1085, 1382, 1882, 2006, 2625, 2867
AfiI CCNNNNNNNGG 7 cut(s) 513, 1247, 1489, 2274, 2684, 2819, 3187
AflII CTTAAG 1 cut(s) 2375
AflIII ACRYGT 5 cut(s) 100, 1368, 1432, 1724, 2815
AhlI ACTAGT 1 cut(s) 227
AjiI CACGTC 2 cut(s) 1244, 1727
AjnI CCWGG 5 cut(s) 307, 1143, 2420, 2968, 3180
AjuI GAANNNNNNNTTGG 2 cut(s) 2005, 2037
AloI GAACNNNNNNTCC 2 cut(s) 1960, 1992
Alw21I GWGCWC 1 cut(s) 2524
Alw26I GTCTC 4 cut(s) 1033, 1500, 1823, 3207
AlwNI CAGNNNCTG 1 cut(s) 2657
AoxI GGCC 7 cut(s) 407, 1948, 1958, 2407, 3178, 3183, 3289
Asp700I GAANNNNTTC 2 cut(s) 2337, 2478
AspS9I GGNCC 7 cut(s) 64, 1480, 1536, 1562, 1959, 2876, 3178
AsuC2I CCSGG 1 cut(s) 3187
AsuHPI GGTGA 3 cut(s) 1916, 1925, 3016
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 5 cut(s) 64, 1480, 1536, 1562, 2876
BaeI ACNNNNGTAYC 2 cut(s) 937, 970
BamHI GGATCC 2 cut(s) 1603, 1637
BanI GGYRCC 1 cut(s) 2549
BarI GAAGNNNNNNTAC 2 cut(s) 1988, 2020
BbrPI CACGTG 1 cut(s) 2816
BbsI GAAGAC 2 cut(s) 1259, 2171
Bbv12I GWGCWC 1 cut(s) 2524
BbvCI CCTCAGC 1 cut(s) 822
BceAI ACGGC 1 cut(s) 1628
BciT130I CCWGG 5 cut(s) 309, 1145, 2422, 2970, 3182
BciVI GTATCC 2 cut(s) 190, 2386
BclI TGATCA 2 cut(s) 1849, 3163
BcnI CCSGG 1 cut(s) 3187
BcoDI GTCTC 4 cut(s) 1033, 1500, 1823, 3207
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 2 cut(s) 228, 677
BfmI CTRYAG 7 cut(s) 484, 588, 845, 912, 1065, 1653, 3320
BfrI CTTAAG 1 cut(s) 2375
BfuAI ACCTGC 1 cut(s) 2955
BfuI GTATCC 2 cut(s) 190, 2386
BglI GCCNNNNNGGC 1 cut(s) 1149
BglII AGATCT 1 cut(s) 3127
BlpI GCTNAGC 2 cut(s) 2253, 3099
Bme1390I CCNGG 6 cut(s) 309, 1145, 2422, 2970, 3182, 3187
Bme18I GGWCC 5 cut(s) 64, 1480, 1536, 1562, 2876
BmgBI CACGTC 2 cut(s) 1244, 1727
BmgT120I GGNCC 7 cut(s) 64, 1480, 1536, 1562, 1959, 2876, 3178
BmiI GGNNCC 4 cut(s) 1455, 1605, 1639, 2551
BmrFI CCNGG 6 cut(s) 309, 1145, 2422, 2970, 3182, 3187
BmrI ACTGGG 2 cut(s) 1452, 1900
BmsI GCATC 4 cut(s) 85, 196, 1207, 1243
BmuI ACTGGG 2 cut(s) 1452, 1900
BpiI GAAGAC 2 cut(s) 1259, 2171
BplI GAGNNNNNCTC 2 cut(s) 815, 847
Bpu10I CCTNAGC 2 cut(s) 822, 1176
Bpu1102I GCTNAGC 2 cut(s) 2253, 3099
Bpu14I TTCGAA 1 cut(s) 543
BpuMI CCSGG 1 cut(s) 3187
BsaAI YACGTR 1 cut(s) 2816
BsaBI GATNNNNATC 1 cut(s) 726
BsaI GGTCTC 2 cut(s) 1823, 3207
BsaJI CCNNGG 9 cut(s) 194, 1052, 1411, 1539, 1822, 2195, 2421, 2969, 3186
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 573, 603
Bsc4I CCNNNNNNNGG 7 cut(s) 513, 1247, 1489, 2274, 2684, 2819, 3187
Bse118I RCCGGY 1 cut(s) 2552
Bse1I ACTGG 9 cut(s) 30, 152, 371, 481, 1047, 1432, 1458, 1895, 2314
Bse3DI GCAATG 5 cut(s) 1479, 1764, 1777, 1982, 2785
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 5 cut(s) 309, 1145, 2422, 2970, 3182
BseDI CCNNGG 9 cut(s) 194, 1052, 1411, 1539, 1822, 2195, 2421, 2969, 3186
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 7 cut(s) 513, 1247, 1489, 2274, 2684, 2819, 3187
BseMI GCAATG 5 cut(s) 1479, 1764, 1777, 1982, 2785
BseMII CTCAG 3 cut(s) 345, 813, 3090
BseNI ACTGG 9 cut(s) 30, 152, 371, 481, 1047, 1432, 1458, 1895, 2314
BseRI GAGGAG 3 cut(s) 1962, 2243, 3153
BseYI CCCAGC 2 cut(s) 2094, 2274
BsgI GTGCAG 3 cut(s) 174, 252, 377
Bsh1285I CGRYCG 1 cut(s) 1966
BshFI GGCC 7 cut(s) 409, 1950, 1960, 2409, 3180, 3185, 3291
BshNI GGYRCC 1 cut(s) 2549
BsiEI CGRYCG 1 cut(s) 1966
BsiHKAI GWGCWC 1 cut(s) 2524
BsiSI CCGG 3 cut(s) 2553, 3186, 3288
BslFI GGGAC 5 cut(s) 670, 1703, 2702, 2774, 2939
BslI CCNNNNNNNGG 7 cut(s) 513, 1247, 1489, 2274, 2684, 2819, 3187
BsmAI GTCTC 4 cut(s) 1033, 1500, 1823, 3207
BsmFI GGGAC 5 cut(s) 670, 1703, 2702, 2774, 2939
BsmI GAATGC 2 cut(s) 747, 2780
BsnI GGCC 7 cut(s) 409, 1950, 1960, 2409, 3180, 3185, 3291
Bso31I GGTCTC 2 cut(s) 1823, 3207
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 2524
Bsp1407I TGTACA 3 cut(s) 1083, 1380, 2623
Bsp1720I GCTNAGC 2 cut(s) 2253, 3099
Bsp19I CCATGG 1 cut(s) 1411
BspACI CCGC 5 cut(s) 1348, 1902, 2544, 2709, 2804
BspANI GGCC 7 cut(s) 409, 1950, 1960, 2409, 3180, 3185, 3291
BspCNI CTCAG 3 cut(s) 346, 814, 3091
BspLI GGNNCC 4 cut(s) 1455, 1605, 1639, 2551
BspMAI CTGCAG 1 cut(s) 1069
BspMI ACCTGC 1 cut(s) 2955
BspT104I TTCGAA 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 2549
BspTI CTTAAG 1 cut(s) 2375
BspTNI GGTCTC 2 cut(s) 1823, 3207
BsrDI GCAATG 5 cut(s) 1479, 1764, 1777, 1982, 2785
BsrFI RCCGGY 1 cut(s) 2552
BsrGI TGTACA 3 cut(s) 1083, 1380, 2623
BsrI ACTGG 9 cut(s) 30, 152, 371, 481, 1047, 1432, 1458, 1895, 2314
BssAI RCCGGY 1 cut(s) 2552
BssECI CCNNGG 9 cut(s) 194, 1052, 1411, 1539, 1822, 2195, 2421, 2969, 3186
BssT1I CCWWGG 5 cut(s) 194, 1052, 1411, 1539, 1822
Bst2UI CCWGG 5 cut(s) 309, 1145, 2422, 2970, 3182
Bst6I CTCTTC 2 cut(s) 83, 2866
BstAFI CTTAAG 1 cut(s) 2375
BstAUI TGTACA 3 cut(s) 1083, 1380, 2623
BstBAI YACGTR 1 cut(s) 2816
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 6 cut(s) 254, 1522, 2265, 2322, 2520, 2577
BstDEI CTNAG 7 cut(s) 37, 295, 354, 822, 1176, 2253, 3099
BstDSI CCRYGG 2 cut(s) 1411, 2195
BstMAI GTCTC 4 cut(s) 1033, 1500, 1823, 3207
BstMCI CGRYCG 1 cut(s) 1966
BstMWI GCNNNNNNNGC 4 cut(s) 566, 948, 1149, 2714
BstNI CCWGG 5 cut(s) 309, 1145, 2422, 2970, 3182
BstNSI RCATGY 8 cut(s) 104, 1372, 1381, 1436, 1757, 2267, 2274, 2954
BstSCI CCNGG 6 cut(s) 307, 1143, 2420, 2968, 3180, 3185
BstSFI CTRYAG 7 cut(s) 484, 588, 845, 912, 1065, 1653, 3320
BstV2I GAAGAC 2 cut(s) 1259, 2171
BstX2I RGATCY 7 cut(s) 134, 959, 1603, 1637, 1711, 2126, 3127
BstXI CCANNNNNNTGG 2 cut(s) 1059, 2514
BstYI RGATCY 7 cut(s) 134, 959, 1603, 1637, 1711, 2126, 3127
BsuI GTATCC 2 cut(s) 190, 2386
BsuRI GGCC 7 cut(s) 409, 1950, 1960, 2409, 3180, 3185, 3291
BtgI CCRYGG 2 cut(s) 1411, 2195
BtrI CACGTC 2 cut(s) 1244, 1727
BtsI GCAGTG 2 cut(s) 336, 958
BtsIMutI CAGTG 5 cut(s) 336, 898, 958, 1888, 2689
BveI ACCTGC 1 cut(s) 2955
Cac8I GCNNGC 6 cut(s) 254, 1522, 2265, 2322, 2520, 2577
CaiI CAGNNNCTG 1 cut(s) 2657
Cfr10I RCCGGY 1 cut(s) 2552
Cfr13I GGNCC 7 cut(s) 64, 1480, 1536, 1562, 1959, 2876, 3178
Csp6I GTAC 8 cut(s) 674, 946, 1084, 1381, 1881, 2005, 2624, 2866
CviQI GTAC 8 cut(s) 674, 946, 1084, 1381, 1881, 2005, 2624, 2866
DdeI CTNAG 7 cut(s) 37, 295, 354, 822, 1176, 2253, 3099
DraI TTTAAA 1 cut(s) 1932
DrdI GACNNNNNNGTC 1 cut(s) 2951
DseDI GACNNNNNNGTC 1 cut(s) 2951
EaeI YGGCCR 3 cut(s) 2407, 3183, 3289
Eam1104I CTCTTC 2 cut(s) 83, 2866
EarI CTCTTC 2 cut(s) 83, 2866
EciI GGCGGA 2 cut(s) 2559, 2793
Eco130I CCWWGG 5 cut(s) 194, 1052, 1411, 1539, 1822
Eco31I GGTCTC 2 cut(s) 1823, 3207
Eco32I GATATC 1 cut(s) 3298
Eco47I GGWCC 5 cut(s) 64, 1480, 1536, 1562, 2876
Eco57I CTGAAG 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 2816
EcoRI GAATTC 1 cut(s) 2087
EcoRII CCWGG 5 cut(s) 307, 1143, 2420, 2968, 3180
EcoRV GATATC 1 cut(s) 3298
EcoT14I CCWWGG 5 cut(s) 194, 1052, 1411, 1539, 1822
ErhI CCWWGG 5 cut(s) 194, 1052, 1411, 1539, 1822
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 5 cut(s) 670, 1703, 2702, 2774, 2939
FauI CCCGC 1 cut(s) 1355
FauNDI CATATG 1 cut(s) 1897
FbaI TGATCA 2 cut(s) 1849, 3163
FblI GTMKAC 3 cut(s) 610, 1652, 2850
FspBI CTAG 2 cut(s) 228, 677
GsaI CCCAGC 2 cut(s) 2098, 2278
HaeIII GGCC 7 cut(s) 409, 1950, 1960, 2409, 3180, 3185, 3291
HapII CCGG 3 cut(s) 2553, 3186, 3288
HincII GTYRAC 2 cut(s) 23, 611
HindII GTYRAC 2 cut(s) 23, 611
HindIII AAGCTT 2 cut(s) 998, 2255
HpaII CCGG 3 cut(s) 2553, 3186, 3288
HphI GGTGA 3 cut(s) 1916, 1925, 3016
Hpy188I TCNGA 5 cut(s) 355, 1446, 1716, 3137, 3228
HpyCH4IV ACGT 7 cut(s) 112, 1243, 1726, 1917, 2763, 2815, 2994
HpyF10VI GCNNNNNNNGC 4 cut(s) 566, 948, 1149, 2714
HpyF3I CTNAG 7 cut(s) 37, 295, 354, 822, 1176, 2253, 3099
HpySE526I ACGT 7 cut(s) 112, 1243, 1726, 1917, 2763, 2815, 2994
Ksp22I TGATCA 2 cut(s) 1849, 3163
LmnI GCTCC 2 cut(s) 939, 2197
LweI GCATC 4 cut(s) 85, 196, 1207, 1243
MaeI CTAG 2 cut(s) 228, 677
MaeII ACGT 7 cut(s) 112, 1243, 1726, 1917, 2763, 2815, 2994
MaeIII GTNAC 9 cut(s) 103, 1617, 1904, 1913, 2057, 2647, 2942, 3004, 3083
MfeI CAATTG 2 cut(s) 126, 1800
MflI RGATCY 7 cut(s) 134, 959, 1603, 1637, 1711, 2126, 3127
MhlI GDGCHC 1 cut(s) 2524
MlyI GAGTC 2 cut(s) 275, 2931
MmeI TCCRAC 4 cut(s) 207, 1458, 1469, 2899
MroXI GAANNNNTTC 2 cut(s) 2337, 2478
MslI CAYNNNNRTG 6 cut(s) 756, 1382, 1676, 2064, 2109, 2884
MspA1I CMGCKG 5 cut(s) 391, 1183, 1350, 2909, 3098
MspCI CTTAAG 1 cut(s) 2375
MspI CCGG 3 cut(s) 2553, 3186, 3288
MspR9I CCNGG 6 cut(s) 309, 1145, 2422, 2970, 3182, 3187
MunI CAATTG 2 cut(s) 126, 1800
Mva1269I GAATGC 2 cut(s) 747, 2780
MvaI CCWGG 5 cut(s) 309, 1145, 2422, 2970, 3182
MwoI GCNNNNNNNGC 4 cut(s) 566, 948, 1149, 2714
NciI CCSGG 1 cut(s) 3187
NcoI CCATGG 1 cut(s) 1411
NdeI CATATG 1 cut(s) 1897
NlaIV GGNNCC 4 cut(s) 1455, 1605, 1639, 2551
NmuCI GTSAC 7 cut(s) 103, 1904, 1913, 2057, 2647, 2942, 3004
NspI RCATGY 8 cut(s) 104, 1372, 1381, 1436, 1757, 2267, 2274, 2954
NspV TTCGAA 1 cut(s) 543
PaeI GCATGC 1 cut(s) 2267
PciI ACATGT 3 cut(s) 100, 1368, 1432
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PctI GAATGC 2 cut(s) 747, 2780
PdmI GAANNNNTTC 2 cut(s) 2337, 2478
PflMI CCANNNNNTGG 3 cut(s) 513, 2274, 2684
PfoI TCCNGGA 1 cut(s) 307
Ple19I CGATCG 1 cut(s) 1966
PleI GAGTC 2 cut(s) 274, 2930
PmaCI CACGTG 1 cut(s) 2816
PmlI CACGTG 1 cut(s) 2816
PpsI GAGTC 2 cut(s) 274, 2930
Ppu21I YACGTR 1 cut(s) 2816
PscI ACATGT 3 cut(s) 100, 1368, 1432
Psp6I CCWGG 5 cut(s) 307, 1143, 2420, 2968, 3180
PspCI CACGTG 1 cut(s) 2816
PspFI CCCAGC 2 cut(s) 2094, 2274
PspGI CCWGG 5 cut(s) 307, 1143, 2420, 2968, 3180
PspN4I GGNNCC 4 cut(s) 1455, 1605, 1639, 2551
PspPI GGNCC 7 cut(s) 64, 1480, 1536, 1562, 1959, 2876, 3178
PsrI GAACNNNNNNTAC 4 cut(s) 666, 698, 1636, 1668
PstI CTGCAG 1 cut(s) 1069
PstNI CAGNNNCTG 1 cut(s) 2657
PsuI RGATCY 7 cut(s) 134, 959, 1603, 1637, 1711, 2126, 3127
PvuI CGATCG 1 cut(s) 1966
PvuII CAGCTG 4 cut(s) 391, 1183, 2909, 3098
RsaI GTAC 8 cut(s) 675, 947, 1085, 1382, 1882, 2006, 2625, 2867
RsaNI GTAC 8 cut(s) 674, 946, 1084, 1381, 1881, 2005, 2624, 2866
RseI CAYNNNNRTG 6 cut(s) 756, 1382, 1676, 2064, 2109, 2884
SalI GTCGAC 1 cut(s) 609
Sau96I GGNCC 7 cut(s) 64, 1480, 1536, 1562, 1959, 2876, 3178
SchI GAGTC 2 cut(s) 275, 2931
ScrFI CCNGG 6 cut(s) 309, 1145, 2422, 2970, 3182, 3187
SduI GDGCHC 1 cut(s) 2524
SfaNI GCATC 4 cut(s) 85, 196, 1207, 1243
SfcI CTRYAG 7 cut(s) 484, 588, 845, 912, 1065, 1653, 3320
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 5 cut(s) 64, 1480, 1536, 1562, 2876
SmiMI CAYNNNNRTG 6 cut(s) 756, 1382, 1676, 2064, 2109, 2884
SmlI CTYRAG 1 cut(s) 2375
SmoI CTYRAG 1 cut(s) 2375
SpeI ACTAGT 1 cut(s) 227
SphI GCATGC 1 cut(s) 2267
SsiI CCGC 5 cut(s) 1348, 1902, 2544, 2709, 2804
SspI AATATT 3 cut(s) 1123, 1402, 1790
SspMI CTAG 2 cut(s) 228, 677
StyD4I CCNGG 6 cut(s) 307, 1143, 2420, 2968, 3180, 3185
StyI CCWWGG 5 cut(s) 194, 1052, 1411, 1539, 1822
TaiI ACGT 7 cut(s) 115, 1246, 1729, 1920, 2766, 2818, 2997
TaqI TCGA 6 cut(s) 543, 552, 610, 812, 1419, 1966
TatI WGTACW 4 cut(s) 673, 1083, 1380, 2623
TauI GCSGC 1 cut(s) 2711
TscAI CASTG 5 cut(s) 343, 898, 958, 1895, 2689
TseFI GTSAC 7 cut(s) 103, 1904, 1913, 2057, 2647, 2942, 3004
Tsp45I GTSAC 7 cut(s) 103, 1904, 1913, 2057, 2647, 2942, 3004
TspGWI ACGGA 4 cut(s) 411, 674, 2184, 2883
TspRI CASTG 5 cut(s) 343, 898, 958, 1895, 2689
Van91I CCANNNNNTGG 3 cut(s) 513, 2274, 2684
Vha464I CTTAAG 1 cut(s) 2375
VpaK11BI GGWCC 5 cut(s) 64, 1480, 1536, 1562, 2876
XceI RCATGY 8 cut(s) 104, 1372, 1381, 1436, 1757, 2267, 2274, 2954
XcmI CCANNNNNNNNNTGG 1 cut(s) 3100
XmiI GTMKAC 3 cut(s) 610, 1652, 2850
XmnI GAANNNNTTC 2 cut(s) 2337, 2478
XspI CTAG 2 cut(s) 228, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.