MD02G1287500.v1.1

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010440
Physical Location & Seq
Reverse (-)
34413595 .. 34423239
9645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1287500.v1.1.491

Sequence Viewer

Length: 3342 bp
ATGGCTTCTTCTTTGCCGGGTCAACTCGTTTCCCTGTTAGAGAATGGGCAGCATCATGTGTGGGAGGACCAGTCGATCATCAAGTGGCGGAAGAGAGACGCCCATGTCCCTTTACGCTGCCATGAATCAGTTGATGGGTCTCTTAAATATTGGTATGAACGCAACAAAGTGAGTTTTGTGGTATCCAACTCTGCTGTTTGGAATGATGATGCTGTTGTTGGAGCTCTTGACAGCGCGGCTCTTTGGGTCAAGGGCTTGCCTTTTGTCAAGTCCTTGTCAGGCTATTGGAAATTCTTCTTGGCTTCCAATCCTAAAAATGTTCCCTTAAATTTTCATGACTCTGCATTTCATGATATACCGTGGGAAACTTTGCCCGTTCCTTCAAATTGGCAGATGCATGGATTTGATCGGCCTATTTATACAAATGTTGTGTATCCATTTCCACTGGATCCACCTTTTGTTCATGTGGACAATCCTACTGGCTGTTATAGGACATACTTTGACATTCCTAAAGAATGGAGTGGCCGCAGGATTTTTTTGCACTTTGAAGCTGTTGATTCTGCTTTCTGTGCATGGGTGAATGGGGTCCCGATAGGATATAGTCAGGACAGCAGACTTCCTGCTGAATTTGAAATCACCGATTATTGTTACCCATTCAGTACGGACAAGAAAAATGTACTAGCTGTTCAAGTTTTCAGATGGAGTGATGGCTCTTACCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTCCTTTCCAAGCCACAGGTATTCATCGCAGACTATTTTTTCAAATCAACTCTGGCCGAGGATTTTTCTTATGCAGACATACAGGTTGAAGTGAAAATAGATAACTCCAGAGAAACATCTAAAGATAGTTTTCTTCCCAACTACACAATAGAAGCTTCATTATTTGACACGGCTAGCTGGTACAGTACTGATGGATATGCTGATCTGGCCTCATCTAATGTGGCTAGTTTAAAGCTTAATCCCTCACCCAGCACAAGCCTTGGATTTCATGGTTATTGGCTGGAAGGGAGACTGGAAATGCCGAGGCTTTGGTCTGCCGAGCAACCAAATCTGTACACACTTGCTGTTATATTGAAAGATGCATCTGGTAACCTTGTGGACTGTGAATCATGCCTCGTAGGCATACGACAAGTATCAAAAGCCCCCAAACAGCTGCTTGTTAATGGGCGTCCAATAATAATAAGGGGTGTAAACAGGCATGAGCATCATCCACGTCTGGGAAAAACGAACATAGAATCCTGCATGGTTAAGGATTTGATTTTAATGAAGCAATATAATTTCAATGCTGTGAGAAACAGTCATTATCCCCAACATCCCCGTTGGTATGAGCTTTGTGATTTGTTTGGCATGTACATGATAGATGAAGCCAACATTGAGACCCATGGTTTTGATTATTCTGGGCATGTGAAGCATCCTACATTAGAACCAAGCTGGGCTACTGCAATGATAGACCGTGTGATAGGCATGGTTGAGAGAGACAAAAATCATGCTTGCATTCTCTCCTGGTCCCTAGGAAATGAAGCGGGATATGGACCTAATCATTCTGCTTCAGCCGGCTGGATTCGTGGCAAGGATCCCTCACGGCTGTTACATTACGAAGGGGGTGGATCCAGAACTCCATCCACAGATATTGTATGTCCCATGTATATGCGGGTCTGGGACATTGTGAAGATTGCAAAAGATCCAAATGAAACACGTCCTTTGATATTGTGCGAGTATTCACATGCAATGGGAAACAGCAGTGGCAATATACATAAATATTGGGAAGCAATTGATAGCACATTTGGTCTCCAAGGAGGCTTTATCTGGGAGTGGGTTGATCAGGGCCTATTGAAGGAAAGTGCAGATGGTAGTAAGCACTGGGCTTATGGGGGTGACTTTGGAGATGTTCCTAATGATCTGAACTTCTGTTTGAATGGCCTTGTATGGCCAGATCGAACTCCTCATCCTGCAATGCATGAAGTCAAGTATGTGTACCAACCAATCAAGGTTTCATTCAGGGAAGAAGCAGTAAAGATAACAAATACTCACTTTTATGAAACAACACAAGGATTGGAGTTCAGCTGGTCTGCTCATGGGGATGGATATAAACTTGGATCTGGAATTCTGCCTCTTCCTTTGATTGAACCTCAGAAGAGTTTTTCTATTGAGTGGAAGTCAGCTCCATGGTATCCTCTATGGACTTCATCATTTGCGGAGGAATATTTTCTAACAATAACTGCTAAGCTTTTGCATTCCACAAAGTGGGTTAAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTTCCAAGAGAGAAATTGTTCCTCATGTCATCAAGACTAAAGAAGCTACATTCATCAGTGAAATTCTTGGGGATACAATTAAAGTGAGTCAGCAGAACCTTTGGGAGATAATATTGAATGTTAAAACGGGAGCAGTTGAAAGCTGGAAGGTTGAAGGAGTTTCTTTGATGACGAAAGGCATATTTCCATGTTTCTGGCGAGCACCCACAGATAATGACAAAGGAGGAGGTGACAGTAGTTATTTCTCCTTGTGGAAAGCTGCTCGTATTGATAGCCTCAATTATATCACCAAAAGCTGTTCTATACAAACTAAGACTGACCATCTTGTGAGAGTAGCTGCGGTTTTTCTTGGTGTTCCCAAAAGTGAGGAGGGTTCTTTATCCAAGGAGGAAAGCGCATTAATTGAGATTGATGTGATTTACACAATTTACGGTTCAGGAGATGTTGTCATTGAATGCAATACAAGACCAAGTTCGAACCTTCCACCTCTGCCACGTGTTGGAGTTGAGTTCCATTTGGATAAGTCGATGGACCAGATTAAATGGTATGGAAGAGGACCGTTTGAATGCTATCCGGACAGAAAAGCAGCTGCTCATGTTGCGGTCTACGAGCAGAATGTGGGTGATATGCATGTCCCTTACATAGTTCCTGGGGAATGTTCAGGTAGGGCGGATGTTAGATGGGTGACATTTCAAAACAAGGATGGTTTCGGAATCTATGCTTCAATATATGGCAGCTCTCCACCTATGCAAATAAATGCAAGTTATTACACCACAGCTGAGCTTGACCGTGCAACGCACAATCACAACCTTGTCAAAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTGGCTGGGGATGATAGTTGGTCCCCCTGCGTCCACAAGGAGTATCTGATTCCTGCTGTTCCGTATTCATTCTCAATCAGGCTGTGCCCAATAACTCCAGCAACCTCTGGCCTCGATGTCTACAAATCTCAACTTCAATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1114

Amino Acids

125.9

Weight (kDa)

5.88

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 261 5.6e-53 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 263 - 392 1e-12 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 399 - 679 1.7e-95 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 687 - 780 2.8e-19 Beta-galactosidase, domain 4
Bgal_small_N PF02929 813 - 1094 1.7e-73 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
AccB7I CCANNNNNTGG 2 cut(s) 2292, 2837
AccI GTMKAC 2 cut(s) 2943, 3318
AccII CGCG 1 cut(s) 236
AccIII TCCGGA 1 cut(s) 2911
AciI CCGC 9 cut(s) 88, 236, 526, 1571, 1699, 2243, 2678, 2939, 3008
AclWI GGATC 8 cut(s) 443, 456, 1616, 1629, 1650, 1663, 1724, 2152
AcoI YGGCCR 3 cut(s) 523, 822, 1976
AcsI RAATTY 5 cut(s) 290, 328, 626, 2151, 2400
AcuI CTGAAG 1 cut(s) 1581
AcvI CACGTG 1 cut(s) 2834
AcyI GRCGYC 2 cut(s) 99, 1216
AdeI CACNNNGTG 1 cut(s) 2292
AfaI GTAC 7 cut(s) 661, 678, 950, 955, 1103, 1400, 2024
AfiI CCNNNNNNNGG 5 cut(s) 1265, 1882, 2292, 2837, 3205
AflIII ACRYGT 2 cut(s) 1742, 2833
AjiI CACGTC 2 cut(s) 1262, 1745
AjnI CCWGG 3 cut(s) 1550, 2986, 3198
AjuI GAANNNNNNNTTGG 4 cut(s) 281, 313, 2023, 2055
AloI GAACNNNNNNTCC 4 cut(s) 1268, 1300, 1978, 2010
Alw21I GWGCWC 2 cut(s) 226, 2542
Alw26I GTCTC 6 cut(s) 90, 144, 1051, 1418, 1518, 1841
AlwI GGATC 8 cut(s) 443, 456, 1616, 1629, 1650, 1663, 1724, 2152
Aor13HI TCCGGA 1 cut(s) 2911
AoxI GGCC 9 cut(s) 410, 523, 822, 975, 1873, 1966, 1976, 3196, 3307
ApeKI GCWGC 8 cut(s) 49, 117, 1201, 2597, 2675, 2924, 2927, 3072
ApoI RAATTY 5 cut(s) 290, 328, 626, 2151, 2400
AseI ATTAAT 1 cut(s) 2738
Asp700I GAANNNNTTC 3 cut(s) 293, 2253, 2355
AspA2I CCTAGG 1 cut(s) 1558
AspLEI GCGC 2 cut(s) 236, 2735
AspS9I GGNCC 9 cut(s) 67, 586, 1554, 1580, 1873, 2869, 2894, 3196, 3219
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 8 cut(s) 589, 628, 1005, 1934, 2579, 2617, 2972, 3034
AsuII TTCGAA 1 cut(s) 2813
AsuNHI GCTAGC 1 cut(s) 941
AvaII GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
AvrII CCTAGG 1 cut(s) 1558
BaeGI GKGCMC 1 cut(s) 3287
BalI TGGCCA 1 cut(s) 1978
BamHI GGATCC 3 cut(s) 448, 1621, 1655
BanII GRGCYC 1 cut(s) 226
BbrPI CACGTG 1 cut(s) 2834
Bbv12I GWGCWC 2 cut(s) 226, 2542
BbvI GCAGC 8 cut(s) 61, 104, 1188, 2584, 2662, 2914, 2936, 3084
BceAI ACGGC 2 cut(s) 954, 1646
BciT130I CCWGG 3 cut(s) 1552, 2988, 3200
BciVI GTATCC 4 cut(s) 193, 444, 2229, 2404
BclI TGATCA 2 cut(s) 1867, 3181
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 6 cut(s) 90, 144, 1051, 1418, 1518, 1841
BfaI CTAG 4 cut(s) 680, 942, 993, 1559
BfuI GTATCC 4 cut(s) 193, 444, 2229, 2404
BglI GCCNNNNNGGC 1 cut(s) 1167
BlnI CCTAGG 1 cut(s) 1558
BlpI GCTNAGC 2 cut(s) 2271, 3117
BmcAI AGTACT 1 cut(s) 955
Bme1390I CCNGG 4 cut(s) 18, 1552, 2988, 3200
Bme18I GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
BmgBI CACGTC 2 cut(s) 1262, 1745
BmgT120I GGNCC 9 cut(s) 67, 586, 1554, 1580, 1873, 2869, 2894, 3196, 3219
BmiI GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
BmrFI CCNGG 4 cut(s) 18, 1552, 2988, 3200
BmrI ACTGGG 1 cut(s) 1918
BmsI GCATC 7 cut(s) 61, 199, 384, 1117, 1139, 1261, 1468
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 1 cut(s) 1918
BpmI CTGGAG 2 cut(s) 859, 3279
Bpu1102I GCTNAGC 2 cut(s) 2271, 3117
Bpu14I TTCGAA 1 cut(s) 2813
BpuMI CCSGG 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 2834
BsaBI GATNNNNATC 1 cut(s) 729
BsaHI GRCGYC 2 cut(s) 99, 1216
BsaI GGTCTC 3 cut(s) 144, 1418, 1841
BsaWI WCCGGW 1 cut(s) 2911
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 2769, 2799
Bsc4I CCNNNNNNNGG 5 cut(s) 1265, 1882, 2292, 2837, 3205
Bse118I RCCGGY 1 cut(s) 1601
Bse1I ACTGG 6 cut(s) 70, 450, 484, 1065, 1913, 2332
Bse3DI GCAATG 3 cut(s) 1497, 1782, 2007
Bse8I GATNNNNATC 1 cut(s) 729
BseAI TCCGGA 1 cut(s) 2911
BseBI CCWGG 3 cut(s) 1552, 2988, 3200
BseGI GGATG 9 cut(s) 1255, 1360, 1459, 1667, 1993, 2134, 3016, 3046, 3214
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 5 cut(s) 1265, 1882, 2292, 2837, 3205
BseMI GCAATG 3 cut(s) 1497, 1782, 2007
BseMII CTCAG 2 cut(s) 2192, 3108
BseNI ACTGG 6 cut(s) 70, 450, 484, 1065, 1913, 2332
BseRI GAGGAG 3 cut(s) 1980, 2577, 2720
BseSI GKGCMC 1 cut(s) 3287
BseXI GCAGC 8 cut(s) 61, 104, 1188, 2584, 2662, 2914, 2936, 3084
BseYI CCCAGC 3 cut(s) 1016, 1479, 3203
BsgI GTGCAG 1 cut(s) 1911
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 9 cut(s) 412, 525, 824, 977, 1875, 1968, 1978, 3198, 3309
BsiHKAI GWGCWC 2 cut(s) 226, 2542
BsiSI CCGG 3 cut(s) 17, 1602, 2912
BslFI GGGAC 7 cut(s) 92, 572, 1540, 1671, 1721, 2957, 3205
BslI CCNNNNNNNGG 5 cut(s) 1265, 1882, 2292, 2837, 3205
BsmAI GTCTC 6 cut(s) 90, 144, 1051, 1418, 1518, 1841
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 7 cut(s) 92, 572, 1540, 1671, 1721, 2957, 3205
BsmI GAATGC 5 cut(s) 750, 1542, 2281, 2798, 2909
BsnI GGCC 9 cut(s) 412, 525, 824, 977, 1875, 1968, 1978, 3198, 3309
Bso31I GGTCTC 3 cut(s) 144, 1418, 1841
Bsp119I TTCGAA 1 cut(s) 2813
Bsp1286I GDGCHC 3 cut(s) 226, 2542, 3287
Bsp13I TCCGGA 1 cut(s) 2911
Bsp1407I TGTACA 2 cut(s) 1101, 1398
Bsp1720I GCTNAGC 2 cut(s) 2271, 3117
Bsp19I CCATGG 2 cut(s) 1429, 2213
BspACI CCGC 9 cut(s) 88, 236, 526, 1571, 1699, 2243, 2678, 2939, 3008
BspANI GGCC 9 cut(s) 412, 525, 824, 977, 1875, 1968, 1978, 3198, 3309
BspCNI CTCAG 2 cut(s) 2191, 3109
BspEI TCCGGA 1 cut(s) 2911
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 2 cut(s) 334, 349
BspLI GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 8 cut(s) 443, 456, 1616, 1629, 1650, 1663, 1724, 2152
BspT104I TTCGAA 1 cut(s) 2813
BspTNI GGTCTC 3 cut(s) 144, 1418, 1841
BsrDI GCAATG 3 cut(s) 1497, 1782, 2007
BsrFI RCCGGY 1 cut(s) 1601
BsrGI TGTACA 2 cut(s) 1101, 1398
BsrI ACTGG 6 cut(s) 70, 450, 484, 1065, 1913, 2332
BssAI RCCGGY 1 cut(s) 1601
BssNI GRCGYC 2 cut(s) 99, 1216
BssT1I CCWWGG 6 cut(s) 1027, 1429, 1558, 1840, 2213, 2721
Bst2UI CCWGG 3 cut(s) 1552, 2988, 3200
Bst4CI ACNGT 9 cut(s) 360, 953, 1151, 1346, 1502, 2573, 2771, 2898, 3128
Bst6I CTCTTC 4 cut(s) 86, 2166, 2177, 2884
BstACI GRCGYC 2 cut(s) 99, 1216
BstAUI TGTACA 2 cut(s) 1101, 1398
BstBAI YACGTR 1 cut(s) 2834
BstBI TTCGAA 1 cut(s) 2813
BstC8I GCNNGC 5 cut(s) 257, 943, 1540, 1603, 2538
BstDEI CTNAG 4 cut(s) 2178, 2271, 2649, 3117
BstDSI CCRYGG 3 cut(s) 359, 1429, 2213
BstEII GGTNACC 1 cut(s) 1136
BstENI CCTNNNNNAGG 1 cut(s) 1880
BstF5I GGATG 9 cut(s) 1255, 1360, 1459, 1667, 1993, 2134, 3016, 3046, 3214
BstFNI CGCG 1 cut(s) 236
BstHHI GCGC 2 cut(s) 236, 2735
BstMAI GTCTC 6 cut(s) 90, 144, 1051, 1418, 1518, 1841
BstMWI GCNNNNNNNGC 5 cut(s) 569, 974, 1167, 1456, 2936
BstNI CCWGG 3 cut(s) 1552, 2988, 3200
BstNSI RCATGY 4 cut(s) 1399, 1454, 1775, 2972
BstPI GGTNACC 1 cut(s) 1136
BstSCI CCNGG 4 cut(s) 16, 1550, 2986, 3198
BstSLI GKGCMC 1 cut(s) 3287
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 8 cut(s) 61, 104, 1188, 2584, 2662, 2914, 2936, 3084
BstX2I RGATCY 5 cut(s) 448, 1621, 1655, 1729, 2144
BstXI CCANNNNNNTGG 1 cut(s) 2532
BstYI RGATCY 5 cut(s) 448, 1621, 1655, 1729, 2144
BsuI GTATCC 4 cut(s) 193, 444, 2229, 2404
BsuRI GGCC 9 cut(s) 412, 525, 824, 977, 1875, 1968, 1978, 3198, 3309
BtgI CCRYGG 3 cut(s) 359, 1429, 2213
BtgZI GCGATG 1 cut(s) 778
BtrI CACGTC 2 cut(s) 1262, 1745
BtsCI GGATG 9 cut(s) 1255, 1360, 1459, 1667, 1993, 2134, 3016, 3046, 3214
BtsI GCAGTG 1 cut(s) 1795
BtsIMutI CAGTG 4 cut(s) 443, 1795, 1906, 2401
Cac8I GCNNGC 5 cut(s) 257, 943, 1540, 1603, 2538
CciI TCATGA 2 cut(s) 334, 349
CfoI GCGC 2 cut(s) 236, 2735
Cfr10I RCCGGY 1 cut(s) 1601
Cfr13I GGNCC 9 cut(s) 67, 586, 1554, 1580, 1873, 2869, 2894, 3196, 3219
CseI GACGC 3 cut(s) 107, 1205, 3217
Csp6I GTAC 7 cut(s) 660, 677, 949, 954, 1102, 1399, 2023
CspCI CAANNNNNGTGG 2 cut(s) 1660, 1695
CviQI GTAC 7 cut(s) 660, 677, 949, 954, 1102, 1399, 2023
DdeI CTNAG 4 cut(s) 2178, 2271, 2649, 3117
DraI TTTAAA 1 cut(s) 999
DraIII CACNNNGTG 1 cut(s) 2292
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 3 cut(s) 523, 822, 1976
Eam1104I CTCTTC 4 cut(s) 86, 2166, 2177, 2884
EarI CTCTTC 4 cut(s) 86, 2166, 2177, 2884
EciI GGCGGA 2 cut(s) 103, 3023
Ecl136II GAGCTC 1 cut(s) 224
Eco130I CCWWGG 6 cut(s) 1027, 1429, 1558, 1840, 2213, 2721
Eco24I GRGCYC 1 cut(s) 226
Eco31I GGTCTC 3 cut(s) 144, 1418, 1841
Eco47I GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
Eco53kI GAGCTC 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 1581
Eco72I CACGTG 1 cut(s) 2834
Eco91I GGTNACC 1 cut(s) 1136
EcoICRI GAGCTC 1 cut(s) 224
EcoNI CCTNNNNNAGG 1 cut(s) 1880
EcoO109I RGGNCCY 2 cut(s) 586, 1873
EcoO65I GGTNACC 1 cut(s) 1136
EcoRI GAATTC 1 cut(s) 2151
EcoRII CCWGG 3 cut(s) 1550, 2986, 3198
EcoT14I CCWWGG 6 cut(s) 1027, 1429, 1558, 1840, 2213, 2721
EcoT22I ATGCAT 4 cut(s) 399, 1132, 2007, 2970
EcoT38I GRGCYC 1 cut(s) 226
ErhI CCWWGG 6 cut(s) 1027, 1429, 1558, 1840, 2213, 2721
Esp3I CGTCTC 1 cut(s) 90
FaqI GGGAC 7 cut(s) 92, 572, 1540, 1671, 1721, 2957, 3205
FauI CCCGC 2 cut(s) 1564, 1692
FbaI TGATCA 2 cut(s) 1867, 3181
FblI GTMKAC 2 cut(s) 2943, 3318
FokI GGATG 9 cut(s) 1242, 1347, 1446, 1654, 1980, 2141, 3023, 3053, 3221
FriOI GRGCYC 1 cut(s) 226
FspBI CTAG 4 cut(s) 680, 942, 993, 1559
GlaI GCGC 2 cut(s) 235, 2734
GsaI CCCAGC 3 cut(s) 1020, 1483, 3207
GsuI CTGGAG 2 cut(s) 859, 3279
HaeIII GGCC 9 cut(s) 412, 525, 824, 977, 1875, 1968, 1978, 3198, 3309
HapII CCGG 3 cut(s) 17, 1602, 2912
HgaI GACGC 3 cut(s) 107, 1205, 3217
HhaI GCGC 2 cut(s) 236, 2735
Hin1I GRCGYC 2 cut(s) 99, 1216
Hin6I GCGC 2 cut(s) 234, 2733
HinP1I GCGC 2 cut(s) 234, 2733
HincII GTYRAC 1 cut(s) 23
HindII GTYRAC 1 cut(s) 23
HindIII AAGCTT 3 cut(s) 921, 1001, 2273
HinfI GANTC 9 cut(s) 125, 338, 557, 1154, 1283, 1609, 2425, 3051, 3247
HpaII CCGG 3 cut(s) 17, 1602, 2912
HphI GGTGA 8 cut(s) 589, 628, 1005, 1934, 2579, 2617, 2972, 3034
Hpy166II GTNNAC 9 cut(s) 23, 469, 1104, 1147, 1240, 2023, 2944, 3232, 3319
Hpy188I TCNGA 5 cut(s) 698, 1950, 2181, 3050, 3246
Hpy8I GTNNAC 9 cut(s) 23, 469, 1104, 1147, 1240, 2023, 2944, 3232, 3319
HpyAV CCTTC 8 cut(s) 390, 1046, 1640, 1876, 2349, 2479, 2486, 2828
HpyCH4III ACNGT 9 cut(s) 360, 953, 1151, 1346, 1502, 2573, 2771, 2898, 3128
HpyCH4IV ACGT 3 cut(s) 1261, 1744, 2833
HpyF10VI GCNNNNNNNGC 5 cut(s) 569, 974, 1167, 1456, 2936
HpyF3I CTNAG 4 cut(s) 2178, 2271, 2649, 3117
HpySE526I ACGT 3 cut(s) 1261, 1744, 2833
Hsp92I GRCGYC 2 cut(s) 99, 1216
HspAI GCGC 2 cut(s) 234, 2733
KflI GGGWCCC 1 cut(s) 586
Kpn2I TCCGGA 1 cut(s) 2911
KroI GCCGGC 1 cut(s) 1601
KroNI GCCGGC 1 cut(s) 1603
Ksp22I TGATCA 2 cut(s) 1867, 3181
LmnI GCTCC 3 cut(s) 221, 2215, 2468
Lsp1109I GCAGC 8 cut(s) 61, 104, 1188, 2584, 2662, 2914, 2936, 3084
LweI GCATC 7 cut(s) 61, 199, 384, 1117, 1139, 1261, 1468
MaeI CTAG 4 cut(s) 680, 942, 993, 1559
MaeII ACGT 3 cut(s) 1261, 1744, 2833
MaeIII GTNAC 6 cut(s) 647, 1136, 1635, 1922, 2567, 3022
MboII GAAGA 9 cut(s) 103, 286, 734, 893, 1729, 2063, 2153, 2194, 2901
MfeI CAATTG 1 cut(s) 1818
MflI RGATCY 5 cut(s) 448, 1621, 1655, 1729, 2144
MhlI GDGCHC 3 cut(s) 226, 2542, 3287
MlsI TGGCCA 1 cut(s) 1978
MluNI TGGCCA 1 cut(s) 1978
MlyI GAGTC 2 cut(s) 332, 2434
MmeI TCCRAC 3 cut(s) 199, 210, 2818
Mox20I TGGCCA 1 cut(s) 1978
Mph1103I ATGCAT 4 cut(s) 399, 1132, 2007, 2970
MroI TCCGGA 1 cut(s) 2911
MroNI GCCGGC 1 cut(s) 1601
MroXI GAANNNNTTC 3 cut(s) 293, 2253, 2355
MscI TGGCCA 1 cut(s) 1978
MslI CAYNNNNRTG 5 cut(s) 759, 1400, 1694, 2082, 2127
Msp20I TGGCCA 1 cut(s) 1978
MspA1I CMGCKG 4 cut(s) 1201, 2112, 2927, 3116
MspI CCGG 3 cut(s) 17, 1602, 2912
MspR9I CCNGG 4 cut(s) 18, 1552, 2988, 3200
MunI CAATTG 1 cut(s) 1818
Mva1269I GAATGC 5 cut(s) 750, 1542, 2281, 2798, 2909
MvaI CCWGG 3 cut(s) 1552, 2988, 3200
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 5 cut(s) 569, 974, 1167, 1456, 2936
NaeI GCCGGC 1 cut(s) 1603
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 2 cut(s) 1429, 2213
NgoMIV GCCGGC 1 cut(s) 1601
NheI GCTAGC 1 cut(s) 941
NlaIV GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
NmeAIII GCCGAG 3 cut(s) 850, 1095, 1111
NmuCI GTSAC 3 cut(s) 1922, 2567, 3022
NsiI ATGCAT 4 cut(s) 399, 1132, 2007, 2970
NspI RCATGY 4 cut(s) 1399, 1454, 1775, 2972
NspV TTCGAA 1 cut(s) 2813
PagI TCATGA 2 cut(s) 334, 349
PctI GAATGC 5 cut(s) 750, 1542, 2281, 2798, 2909
PdiI GCCGGC 1 cut(s) 1603
PdmI GAANNNNTTC 3 cut(s) 293, 2253, 2355
PfeI GAWTC 7 cut(s) 125, 557, 1154, 1283, 1609, 3051, 3247
PflMI CCANNNNNTGG 2 cut(s) 2292, 2837
PleI GAGTC 2 cut(s) 332, 2433
PmaCI CACGTG 1 cut(s) 2834
PmlI CACGTG 1 cut(s) 2834
PpsI GAGTC 2 cut(s) 332, 2433
Ppu21I YACGTR 1 cut(s) 2834
PpuMI RGGWCCY 1 cut(s) 586
PshBI ATTAAT 1 cut(s) 2738
Psp124BI GAGCTC 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 586
Psp6I CCWGG 3 cut(s) 1550, 2986, 3198
PspCI CACGTG 1 cut(s) 2834
PspEI GGTNACC 1 cut(s) 1136
PspFI CCCAGC 3 cut(s) 1016, 1479, 3203
PspGI CCWGG 3 cut(s) 1550, 2986, 3198
PspN4I GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
PspPI GGNCC 9 cut(s) 67, 586, 1554, 1580, 1873, 2869, 2894, 3196, 3219
PspPPI RGGWCCY 1 cut(s) 586
PsrI GAACNNNNNNTAC 2 cut(s) 669, 701
PsuI RGATCY 5 cut(s) 448, 1621, 1655, 1729, 2144
PvuII CAGCTG 4 cut(s) 1201, 2112, 2927, 3116
RsaI GTAC 7 cut(s) 661, 678, 950, 955, 1103, 1400, 2024
RsaNI GTAC 7 cut(s) 660, 677, 949, 954, 1102, 1399, 2023
RseI CAYNNNNRTG 5 cut(s) 759, 1400, 1694, 2082, 2127
SacI GAGCTC 1 cut(s) 226
Sau96I GGNCC 9 cut(s) 67, 586, 1554, 1580, 1873, 2869, 2894, 3196, 3219
ScaI AGTACT 1 cut(s) 955
SchI GAGTC 2 cut(s) 332, 2434
ScrFI CCNGG 4 cut(s) 18, 1552, 2988, 3200
SduI GDGCHC 3 cut(s) 226, 2542, 3287
SfaNI GCATC 7 cut(s) 61, 199, 384, 1117, 1139, 1261, 1468
SfuI TTCGAA 1 cut(s) 2813
SinI GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
SmiMI CAYNNNNRTG 5 cut(s) 759, 1400, 1694, 2082, 2127
SsiI CCGC 9 cut(s) 88, 236, 526, 1571, 1699, 2243, 2678, 2939, 3008
SspI AATATT 4 cut(s) 149, 1808, 2252, 2451
SspMI CTAG 4 cut(s) 680, 942, 993, 1559
SstI GAGCTC 1 cut(s) 226
StyD4I CCNGG 4 cut(s) 16, 1550, 2986, 3198
StyI CCWWGG 6 cut(s) 1027, 1429, 1558, 1840, 2213, 2721
TaaI ACNGT 9 cut(s) 360, 953, 1151, 1346, 1502, 2573, 2771, 2898, 3128
TaiI ACGT 3 cut(s) 1264, 1747, 2836
TaqI TCGA 5 cut(s) 74, 1984, 2813, 2864, 3312
TatI WGTACW 4 cut(s) 676, 953, 1101, 1398
TauI GCSGC 2 cut(s) 239, 528
TfiI GAWTC 7 cut(s) 125, 557, 1154, 1283, 1609, 3051, 3247
TscAI CASTG 4 cut(s) 450, 1795, 1913, 2401
TseFI GTSAC 3 cut(s) 1922, 2567, 3022
TseI GCWGC 8 cut(s) 49, 117, 1201, 2597, 2675, 2924, 2927, 3072
Tsp45I GTSAC 3 cut(s) 1922, 2567, 3022
TspGWI ACGGA 2 cut(s) 677, 3249
TspRI CASTG 4 cut(s) 450, 1795, 1913, 2401
Van91I CCANNNNNTGG 2 cut(s) 2292, 2837
VpaK11BI GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
VspI ATTAAT 1 cut(s) 2738
XagI CCTNNNNNAGG 1 cut(s) 1880
XapI RAATTY 5 cut(s) 290, 328, 626, 2151, 2400
XceI RCATGY 4 cut(s) 1399, 1454, 1775, 2972
XmaJI CCTAGG 1 cut(s) 1558
XmiI GTMKAC 2 cut(s) 2943, 3318
XmnI GAANNNNTTC 3 cut(s) 293, 2253, 2355
XspI CTAG 4 cut(s) 680, 942, 993, 1559
ZrmI AGTACT 1 cut(s) 955
Zsp2I ATGCAT 4 cut(s) 399, 1132, 2007, 2970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.