pycom02g24370

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010440
Physical Location & Seq
Reverse (-)
22488686 .. 22498109
9424 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24370.1

Sequence Viewer

Length: 3342 bp
ATGGATTCTTCTTTGCCGGGTCAACTCGTTTCCCTGTTAGAGAATGGGCAGCATCATGTGTGGGAGGACCAATCGATCATCAAGTGGCGGAAGAGAGACGCCCATGTCCCTTTACGCTGCCATGAATCAGTTGAAGGGTCTCTTAAATATTGGTATGAACGCAACAAAGTGAGTTTTGTGGTATCCAACTCTGCTGTTTGGAATGATGATGCTGTTGTTGGAGCTCTTGACAGCGCGGCTCTTTGGGTCAAGGGCTTGCCTTTTGTCAAGTCCTTGTCAGGCTATTGGAAATTCTTCTTGGCTTCCAGTCCTAAAAATGTTCCCTTAAATTTTTATGACTCTGCATTTCATGATATACAGTGGGAAACTTTGCCTGTTCCTTCAAATTGGCAGATGCATGGATTTGATCGATCTATTTATACAAACGTTGTGTATCCATTTCCACTGGATCCACCTTTTGTTCATGTGGACAATCCTACTGGCTGTTATAGGACATACTTTGACATTCCTAAAGAATGGAATGGCCGCAGGATTTTTTTGCACTTTGAAGCTGTTGATTCTGCTTTCTGTGCGTGGGTGAATGGGGTCCCGATAGGATATAGTCAGGACAGCAGACTTCCTGCTGAATTTGAAATCACCGACTATTGTTACCCATTCAGTACGGACAAGAAAAATGTACTAGCTGTTCAAGTTTTCAGATGGAGTGATGGATCTTACCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTCCTTTCCAAGCCACAGGTATTCATCGCAGACTATTTTTTCAAATCAACTCTGGCCGAGGATTTTTCTTATGCAGACATACAGGTTGAAGTGAAAATAGATAACTCCATAGAAACATCTAAAGATAATATTCTTCCCAACTACACAATAGAAGCTTCATTATTTGACACGGCTAGCTGGTACAGTACTGATGGATATGCTGATCTAGCCTCATCTAATGTGGCTAGTTTAAAGCTTAATCCCTCACCCAGCACAAGTCTTGGATTTCATGGTTATTGGCTGGAAGGGAGACTGGAAATGCCGAGGCTTTGGTCTGCCGAGCAACCAAATCTGTACACACTTGCTGTTATATTGAAAGATGCATCTGGTAACCTCGTGGACTGTGAATCATGCCTCGTAGGCATACGACAAGTATCAAAAGCCCCCAAACAACTGCTTGTTAATGGGCGTCCAATAATAATAAGGGGTGTAAACAGGCATGAGCATCATCCACGTCTGGGAAAAACAAACATAGAATCCTGCATGGTTAAGGATTTGATTTTAATGAAGCAATATAATTTCAATGCTGTGAGAAACAGTCATTATCCCCAACATCCCCGTTGGTATGAGCTTTGTGATTTGTTTGGCATGTACATGATAGATGAAGCCAACATCGAGACCCATGGTTTTGATTATTCTGGGCATGTGAAGCATCCTACATTAGAACCAAGCTGGGCTACTGCAATGATAGACCGTGTGATAGGCATGGTTGAGAGAGACAAAAATCATGCTTGCATTTTCTCCTGGTCCCTGGGAAATGAAGCGGGATATGGACCTAATCATTCTGCTTCAGCCGGCTGGATTCGTGGCAAGGATCCCTCACGGCTGTTACATTACGAAGGGGGTGGATCCAGAACTCCATCCACAGATATTGTATGTCCCATGTATATGCGGGTCTGGGACATTGTGAAGATTGCGAAAGATCCAAATGAAACACGTCCTTTGATATTGTGCGAGTATTCACATGCAATGGGAAACAGCAGTGGCAATATACATAAATATTGGGAAGCAATTGACAGCACATTTGGTCTCCAAGGAGGCTTTATCTGGGAGTGGGTTGATCAGGGCTTATTGAAGGAAGGTGCAGATGGTAGTAAGCATTGGGCTTATGGGGGTGACTTTGGAGATGTTCCTAATGATCTAAACTTCTGTTTGAATGGCCTTGTATGGCCAGATCGAACTCCTCATCCTGCAATGCATGAAGTCAAGTATGTGTACCAACCAATCAAGGTTTCATTCAGGGAAGAAGCAGTAAAGATAACAAATACGCACTTTTATGAAACAACACAAGGATTGGAGTTCAGCTGGTCTGCTCATGGGGATGGATATAAACTTGGATCTGGAATTTTGTCTCTTCCTTTGATTGAACCTCAGAAGAGTTTTTCTATTGAGCAGAAGTCAGCTCCATGGTATCCTCTATGGACTTCATCATTTGCGGAGGAATATTTTTTGACAATAACTGCTAAGCTCTTGCATTCCACAAAGTGGGTTAAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTTCCAAGAGAGAAATTATTCCTCATGTCATCAAGACTAAGGAAGCTACATTCATCAGTGAAATTCTTGGGGATACAATTAAAGTGAGTCGGCAGAACCTTTGGGAGATAATATTGAATGTTAAAGCGGGAGCAGTTGAAAGATGGAAGGTTGAAGGAGTTTCTTTGATGACGAAAGGCATATTTCCATGTTTCTGGCGAGCACCCACAGATAATGACAAAGGAGGAGGTGACAGTAGTTATTTCTCCTTGTGGAAAACTGCTCATATTGATAGCCTCAATTATATCACCAAAAGCTGTTCTATACAAACTAAGACTGACCATCTTGTGAGAGTAGCTGCGGTTTTTCTTGGTGTTCCCAAAAGTGAGGAGGGTTCTTTATCCAAGGAGGAAAGCGCATTAATTGAGATTGATGTGATTTACACAATTTACGGTTCAGGAGATGTTGTCGTTGAATGCAATACAAGACCAAGTTCGAACCTTCCACCTCTGCCACGTGTTGGAGTTGAGTTCCATTTGGATAAGTCAATGGACCAGATTAAATGGTATGGAAGAGGACCGTTTGAATGCTATCCGGACAGAAAAGCAGCTGCTCATGTTGCGGTCTATGAGCAGAATTTGGGCGATATGCATGTCCCTTACATAGTTCCTGGGGAATGTTCAGGTAGGGCGGATGTTAGATGGGTGACATTTCAAAACAAGGATGGTTTCGGAATCTATGCTTCAATTTATGGCAGCTCTCCACCTATGCAAATAAATGCAAGTTGTTACACCACAGCTGAGCTCGACCGTGCAACGCACAATCACGACCTTGTCAAAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTGGCTGGGGATGATAGTTGGTCCCCCTGCGTCCACGACGAGTATCTGATTCCTGCTGTTCCGTATTCATTCTCAATCAGGCTGTGCCCAATAACTCCAGCAACCTCTGGCCTCGACGTCTACAAATCTCAACTTCAATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1114

Amino Acids

125.91

Weight (kDa)

5.76

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 261 3e-52 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 263 - 392 2.5e-12 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 399 - 679 7.7e-95 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 687 - 780 1.2e-18 Beta-galactosidase, domain 4
Bgal_small_N PF02929 813 - 1094 4.9e-72 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
AatII GACGTC 1 cut(s) 3318
AccB7I CCANNNNNTGG 2 cut(s) 2292, 2837
AccI GTMKAC 1 cut(s) 3318
AccII CGCG 1 cut(s) 236
AccIII TCCGGA 1 cut(s) 2911
AclI AACGTT 1 cut(s) 426
AclWI GGATC 9 cut(s) 443, 456, 718, 1616, 1629, 1650, 1663, 1724, 2152
AcoI YGGCCR 3 cut(s) 523, 822, 1976
AcsI RAATTY 6 cut(s) 290, 328, 626, 2151, 2400, 2953
AcuI CTGAAG 1 cut(s) 1581
AcvI CACGTG 1 cut(s) 2834
AcyI GRCGYC 3 cut(s) 99, 1216, 3315
AdeI CACNNNGTG 1 cut(s) 2292
AfaI GTAC 7 cut(s) 661, 678, 950, 955, 1103, 1400, 2024
AfiI CCNNNNNNNGG 4 cut(s) 1265, 2292, 2837, 3205
AflIII ACRYGT 2 cut(s) 1742, 2833
AjiI CACGTC 2 cut(s) 1262, 1745
AjnI CCWGG 4 cut(s) 1550, 1557, 2986, 3198
AjuI GAANNNNNNNTTGG 4 cut(s) 281, 313, 2023, 2055
AloI GAACNNNNNNTCC 2 cut(s) 1978, 2010
Alw21I GWGCWC 3 cut(s) 226, 2542, 3123
Alw26I GTCTC 7 cut(s) 90, 144, 1051, 1418, 1518, 1841, 2163
AlwI GGATC 9 cut(s) 443, 456, 718, 1616, 1629, 1650, 1663, 1724, 2152
Aor13HI TCCGGA 1 cut(s) 2911
AoxI GGCC 6 cut(s) 523, 822, 1966, 1976, 3196, 3307
ApeKI GCWGC 6 cut(s) 49, 117, 2675, 2924, 2927, 3072
ApoI RAATTY 6 cut(s) 290, 328, 626, 2151, 2400, 2953
ArsI GACNNNNNNTTYG 2 cut(s) 1814, 1846
AseI ATTAAT 1 cut(s) 2738
Asp700I GAANNNNTTC 2 cut(s) 293, 2355
AspLEI GCGC 2 cut(s) 236, 2735
AspS9I GGNCC 8 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3196, 3219
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 7 cut(s) 589, 628, 1005, 1934, 2579, 2617, 3034
AsuII TTCGAA 1 cut(s) 2813
AsuNHI GCTAGC 1 cut(s) 941
AvaII GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
BaeGI GKGCMC 1 cut(s) 3287
BalI TGGCCA 1 cut(s) 1978
BamHI GGATCC 3 cut(s) 448, 1621, 1655
BanII GRGCYC 2 cut(s) 226, 3123
BauI CACGAG 1 cut(s) 1142
BbrPI CACGTG 1 cut(s) 2834
Bbv12I GWGCWC 3 cut(s) 226, 2542, 3123
BbvI GCAGC 6 cut(s) 61, 104, 2662, 2914, 2936, 3084
BceAI ACGGC 2 cut(s) 954, 1646
BciT130I CCWGG 4 cut(s) 1552, 1559, 2988, 3200
BciVI GTATCC 4 cut(s) 193, 444, 2229, 2404
BclI TGATCA 2 cut(s) 1867, 3181
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 7 cut(s) 90, 144, 1051, 1418, 1518, 1841, 2163
BfaI CTAG 4 cut(s) 680, 942, 974, 993
BfuI GTATCC 4 cut(s) 193, 444, 2229, 2404
BglI GCCNNNNNGGC 1 cut(s) 1167
BisI GCNGC 8 cut(s) 50, 118, 237, 526, 2676, 2925, 2928, 3073
BlpI GCTNAGC 2 cut(s) 2271, 3117
BlsI GCNGC 8 cut(s) 51, 119, 238, 527, 2677, 2926, 2929, 3074
BmcAI AGTACT 1 cut(s) 955
Bme1390I CCNGG 5 cut(s) 18, 1552, 1559, 2988, 3200
Bme18I GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
BmgBI CACGTC 2 cut(s) 1262, 1745
BmgT120I GGNCC 8 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3196, 3219
BmiI GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
BmrFI CCNGG 5 cut(s) 18, 1552, 1559, 2988, 3200
BmsI GCATC 7 cut(s) 61, 199, 384, 1117, 1139, 1261, 1468
BmtI GCTAGC 1 cut(s) 945
BpmI CTGGAG 1 cut(s) 3279
Bpu1102I GCTNAGC 2 cut(s) 2271, 3117
Bpu14I TTCGAA 1 cut(s) 2813
BpuMI CCSGG 1 cut(s) 18
Bsa29I ATCGAT 2 cut(s) 74, 409
BsaAI YACGTR 1 cut(s) 2834
BsaBI GATNNNNATC 1 cut(s) 729
BsaHI GRCGYC 3 cut(s) 99, 1216, 3315
BsaI GGTCTC 3 cut(s) 144, 1418, 1841
BsaJI CCNNGG 9 cut(s) 825, 1070, 1429, 1557, 1558, 1840, 2213, 2721, 2987
BsaWI WCCGGW 1 cut(s) 2911
BsaXI ACNNNNNCTCC 2 cut(s) 2769, 2799
Bsc4I CCNNNNNNNGG 4 cut(s) 1265, 2292, 2837, 3205
Bse118I RCCGGY 1 cut(s) 1601
Bse1I ACTGG 5 cut(s) 306, 450, 484, 1065, 2332
Bse3DI GCAATG 3 cut(s) 1497, 1782, 2007
Bse8I GATNNNNATC 1 cut(s) 729
BseAI TCCGGA 1 cut(s) 2911
BseBI CCWGG 4 cut(s) 1552, 1559, 2988, 3200
BseCI ATCGAT 2 cut(s) 74, 409
BseDI CCNNGG 9 cut(s) 825, 1070, 1429, 1557, 1558, 1840, 2213, 2721, 2987
BseGI GGATG 9 cut(s) 1255, 1360, 1459, 1667, 1993, 2134, 3016, 3046, 3214
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 4 cut(s) 1265, 2292, 2837, 3205
BseMI GCAATG 3 cut(s) 1497, 1782, 2007
BseMII CTCAG 2 cut(s) 2192, 3108
BseNI ACTGG 5 cut(s) 306, 450, 484, 1065, 2332
BseRI GAGGAG 3 cut(s) 1980, 2577, 2720
BseSI GKGCMC 1 cut(s) 3287
BseXI GCAGC 6 cut(s) 61, 104, 2662, 2914, 2936, 3084
BseYI CCCAGC 3 cut(s) 1016, 1479, 3203
BsgI GTGCAG 1 cut(s) 1911
Bsh1236I CGCG 1 cut(s) 236
Bsh1285I CGRYCG 1 cut(s) 3127
BshFI GGCC 6 cut(s) 525, 824, 1968, 1978, 3198, 3309
BshVI ATCGAT 2 cut(s) 74, 409
BsiEI CGRYCG 1 cut(s) 3127
BsiHKAI GWGCWC 3 cut(s) 226, 2542, 3123
BsiSI CCGG 3 cut(s) 17, 1602, 2912
BslFI GGGAC 7 cut(s) 92, 572, 1540, 1671, 1721, 2957, 3205
BslI CCNNNNNNNGG 4 cut(s) 1265, 2292, 2837, 3205
BsmAI GTCTC 7 cut(s) 90, 144, 1051, 1418, 1518, 1841, 2163
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 7 cut(s) 92, 572, 1540, 1671, 1721, 2957, 3205
BsmI GAATGC 4 cut(s) 750, 2281, 2798, 2909
BsnI GGCC 6 cut(s) 525, 824, 1968, 1978, 3198, 3309
Bso31I GGTCTC 3 cut(s) 144, 1418, 1841
Bsp119I TTCGAA 1 cut(s) 2813
Bsp1286I GDGCHC 4 cut(s) 226, 2542, 3123, 3287
Bsp13I TCCGGA 1 cut(s) 2911
Bsp1407I TGTACA 2 cut(s) 1101, 1398
Bsp1720I GCTNAGC 2 cut(s) 2271, 3117
Bsp19I CCATGG 2 cut(s) 1429, 2213
BspANI GGCC 6 cut(s) 525, 824, 1968, 1978, 3198, 3309
BspCNI CTCAG 2 cut(s) 2191, 3109
BspDI ATCGAT 2 cut(s) 74, 409
BspEI TCCGGA 1 cut(s) 2911
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 9 cut(s) 443, 456, 718, 1616, 1629, 1650, 1663, 1724, 2152
BspT104I TTCGAA 1 cut(s) 2813
BspTNI GGTCTC 3 cut(s) 144, 1418, 1841
BsrDI GCAATG 3 cut(s) 1497, 1782, 2007
BsrFI RCCGGY 1 cut(s) 1601
BsrGI TGTACA 2 cut(s) 1101, 1398
BsrI ACTGG 5 cut(s) 306, 450, 484, 1065, 2332
BssAI RCCGGY 1 cut(s) 1601
BssECI CCNNGG 9 cut(s) 825, 1070, 1429, 1557, 1558, 1840, 2213, 2721, 2987
BssNI GRCGYC 3 cut(s) 99, 1216, 3315
BssSI CACGAG 1 cut(s) 1142
BssT1I CCWWGG 4 cut(s) 1429, 1840, 2213, 2721
Bst2BI CACGAG 1 cut(s) 1142
Bst2UI CCWGG 4 cut(s) 1552, 1559, 2988, 3200
Bst4CI ACNGT 9 cut(s) 360, 953, 1151, 1346, 1502, 2573, 2771, 2898, 3128
Bst6I CTCTTC 4 cut(s) 86, 2166, 2177, 2884
BstACI GRCGYC 3 cut(s) 99, 1216, 3315
BstAUI TGTACA 2 cut(s) 1101, 1398
BstBAI YACGTR 1 cut(s) 2834
BstBI TTCGAA 1 cut(s) 2813
BstC8I GCNNGC 5 cut(s) 257, 943, 1540, 1603, 2538
BstDEI CTNAG 5 cut(s) 2178, 2271, 2376, 2649, 3117
BstDSI CCRYGG 2 cut(s) 1429, 2213
BstEII GGTNACC 1 cut(s) 1136
BstF5I GGATG 9 cut(s) 1255, 1360, 1459, 1667, 1993, 2134, 3016, 3046, 3214
BstFNI CGCG 1 cut(s) 236
BstHHI GCGC 2 cut(s) 236, 2735
BstMAI GTCTC 7 cut(s) 90, 144, 1051, 1418, 1518, 1841, 2163
BstMCI CGRYCG 1 cut(s) 3127
BstMWI GCNNNNNNNGC 5 cut(s) 569, 974, 1167, 1456, 2936
BstNI CCWGG 4 cut(s) 1552, 1559, 2988, 3200
BstNSI RCATGY 4 cut(s) 1399, 1454, 1775, 2972
BstPI GGTNACC 1 cut(s) 1136
BstSCI CCNGG 5 cut(s) 16, 1550, 1557, 2986, 3198
BstSLI GKGCMC 1 cut(s) 3287
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 6 cut(s) 61, 104, 2662, 2914, 2936, 3084
BstX2I RGATCY 6 cut(s) 448, 710, 1621, 1655, 1729, 2144
BstXI CCANNNNNNTGG 1 cut(s) 2532
BstYI RGATCY 6 cut(s) 448, 710, 1621, 1655, 1729, 2144
Bsu15I ATCGAT 2 cut(s) 74, 409
BsuI GTATCC 4 cut(s) 193, 444, 2229, 2404
BsuRI GGCC 6 cut(s) 525, 824, 1968, 1978, 3198, 3309
BsuTUI ATCGAT 2 cut(s) 74, 409
BtgI CCRYGG 2 cut(s) 1429, 2213
BtgZI GCGATG 1 cut(s) 778
BtrI CACGTC 2 cut(s) 1262, 1745
BtsCI GGATG 9 cut(s) 1255, 1360, 1459, 1667, 1993, 2134, 3016, 3046, 3214
BtsI GCAGTG 1 cut(s) 1795
BtsIMutI CAGTG 4 cut(s) 365, 443, 1795, 2401
Cac8I GCNNGC 5 cut(s) 257, 943, 1540, 1603, 2538
CciI TCATGA 1 cut(s) 349
CfoI GCGC 2 cut(s) 236, 2735
Cfr10I RCCGGY 1 cut(s) 1601
Cfr13I GGNCC 8 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3196, 3219
ClaI ATCGAT 2 cut(s) 74, 409
CseI GACGC 3 cut(s) 107, 1205, 3217
Csp6I GTAC 7 cut(s) 660, 677, 949, 954, 1102, 1399, 2023
CspCI CAANNNNNGTGG 2 cut(s) 1660, 1695
CviQI GTAC 7 cut(s) 660, 677, 949, 954, 1102, 1399, 2023
DdeI CTNAG 5 cut(s) 2178, 2271, 2376, 2649, 3117
DraI TTTAAA 1 cut(s) 999
DraIII CACNNNGTG 1 cut(s) 2292
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 3 cut(s) 523, 822, 1976
Eam1104I CTCTTC 4 cut(s) 86, 2166, 2177, 2884
EarI CTCTTC 4 cut(s) 86, 2166, 2177, 2884
EciI GGCGGA 2 cut(s) 103, 3023
Ecl136II GAGCTC 2 cut(s) 224, 3121
Eco130I CCWWGG 4 cut(s) 1429, 1840, 2213, 2721
Eco24I GRGCYC 2 cut(s) 226, 3123
Eco31I GGTCTC 3 cut(s) 144, 1418, 1841
Eco47I GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
Eco53kI GAGCTC 2 cut(s) 224, 3121
Eco57I CTGAAG 1 cut(s) 1581
Eco72I CACGTG 1 cut(s) 2834
Eco91I GGTNACC 1 cut(s) 1136
EcoICRI GAGCTC 2 cut(s) 224, 3121
EcoO109I RGGNCCY 1 cut(s) 586
EcoO65I GGTNACC 1 cut(s) 1136
EcoRII CCWGG 4 cut(s) 1550, 1557, 2986, 3198
EcoT14I CCWWGG 4 cut(s) 1429, 1840, 2213, 2721
EcoT22I ATGCAT 4 cut(s) 399, 1132, 2007, 2970
EcoT38I GRGCYC 2 cut(s) 226, 3123
ErhI CCWWGG 4 cut(s) 1429, 1840, 2213, 2721
Esp3I CGTCTC 1 cut(s) 90
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FaqI GGGAC 7 cut(s) 92, 572, 1540, 1671, 1721, 2957, 3205
FauI CCCGC 3 cut(s) 1564, 1692, 2458
FbaI TGATCA 2 cut(s) 1867, 3181
FblI GTMKAC 1 cut(s) 3318
Fnu4HI GCNGC 8 cut(s) 50, 118, 237, 526, 2676, 2925, 2928, 3073
FokI GGATG 9 cut(s) 1242, 1347, 1446, 1654, 1980, 2141, 3023, 3053, 3221
FriOI GRGCYC 2 cut(s) 226, 3123
Fsp4HI GCNGC 8 cut(s) 50, 118, 237, 526, 2676, 2925, 2928, 3073
FspBI CTAG 4 cut(s) 680, 942, 974, 993
GlaI GCGC 2 cut(s) 235, 2734
GluI GCNGC 8 cut(s) 50, 118, 237, 526, 2676, 2925, 2928, 3073
GsaI CCCAGC 3 cut(s) 1020, 1483, 3207
GsuI CTGGAG 1 cut(s) 3279
HaeIII GGCC 6 cut(s) 525, 824, 1968, 1978, 3198, 3309
HapII CCGG 3 cut(s) 17, 1602, 2912
HgaI GACGC 3 cut(s) 107, 1205, 3217
HhaI GCGC 2 cut(s) 236, 2735
Hin1I GRCGYC 3 cut(s) 99, 1216, 3315
Hin6I GCGC 2 cut(s) 234, 2733
HinP1I GCGC 2 cut(s) 234, 2733
HincII GTYRAC 1 cut(s) 23
HindII GTYRAC 1 cut(s) 23
HindIII AAGCTT 2 cut(s) 921, 1001
HpaII CCGG 3 cut(s) 17, 1602, 2912
HphI GGTGA 7 cut(s) 589, 628, 1005, 1934, 2579, 2617, 3034
Hpy166II GTNNAC 8 cut(s) 23, 469, 1104, 1147, 1240, 2023, 3232, 3319
Hpy188I TCNGA 4 cut(s) 698, 2181, 3050, 3246
Hpy8I GTNNAC 8 cut(s) 23, 469, 1104, 1147, 1240, 2023, 3232, 3319
Hpy99I CGWCG 2 cut(s) 3239, 3317
HpyCH4III ACNGT 9 cut(s) 360, 953, 1151, 1346, 1502, 2573, 2771, 2898, 3128
HpyCH4IV ACGT 5 cut(s) 426, 1261, 1744, 2833, 3315
HpyF10VI GCNNNNNNNGC 5 cut(s) 569, 974, 1167, 1456, 2936
HpyF3I CTNAG 5 cut(s) 2178, 2271, 2376, 2649, 3117
HpySE526I ACGT 5 cut(s) 426, 1261, 1744, 2833, 3315
Hsp92I GRCGYC 3 cut(s) 99, 1216, 3315
HspAI GCGC 2 cut(s) 234, 2733
KflI GGGWCCC 1 cut(s) 586
Kpn2I TCCGGA 1 cut(s) 2911
KroI GCCGGC 1 cut(s) 1601
KroNI GCCGGC 1 cut(s) 1603
Ksp22I TGATCA 2 cut(s) 1867, 3181
LmnI GCTCC 3 cut(s) 221, 2215, 2468
Lsp1109I GCAGC 6 cut(s) 61, 104, 2662, 2914, 2936, 3084
LweI GCATC 7 cut(s) 61, 199, 384, 1117, 1139, 1261, 1468
MaeI CTAG 4 cut(s) 680, 942, 974, 993
MaeII ACGT 5 cut(s) 426, 1261, 1744, 2833, 3315
MaeIII GTNAC 7 cut(s) 647, 1136, 1635, 1922, 2567, 3022, 3104
MboII GAAGA 9 cut(s) 103, 286, 734, 893, 1729, 2063, 2153, 2194, 2901
MfeI CAATTG 1 cut(s) 1818
MflI RGATCY 6 cut(s) 448, 710, 1621, 1655, 1729, 2144
MhlI GDGCHC 4 cut(s) 226, 2542, 3123, 3287
MlsI TGGCCA 1 cut(s) 1978
MluNI TGGCCA 1 cut(s) 1978
MlyI GAGTC 2 cut(s) 332, 2434
MmeI TCCRAC 3 cut(s) 199, 210, 2818
Mox20I TGGCCA 1 cut(s) 1978
Mph1103I ATGCAT 4 cut(s) 399, 1132, 2007, 2970
MroI TCCGGA 1 cut(s) 2911
MroNI GCCGGC 1 cut(s) 1601
MroXI GAANNNNTTC 2 cut(s) 293, 2355
MscI TGGCCA 1 cut(s) 1978
MslI CAYNNNNRTG 5 cut(s) 759, 1400, 1694, 2082, 2127
Msp20I TGGCCA 1 cut(s) 1978
MspA1I CMGCKG 3 cut(s) 2112, 2927, 3116
MspI CCGG 3 cut(s) 17, 1602, 2912
MspR9I CCNGG 5 cut(s) 18, 1552, 1559, 2988, 3200
MunI CAATTG 1 cut(s) 1818
Mva1269I GAATGC 4 cut(s) 750, 2281, 2798, 2909
MvaI CCWGG 4 cut(s) 1552, 1559, 2988, 3200
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 5 cut(s) 569, 974, 1167, 1456, 2936
NaeI GCCGGC 1 cut(s) 1603
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 2 cut(s) 1429, 2213
NgoMIV GCCGGC 1 cut(s) 1601
NheI GCTAGC 1 cut(s) 941
NlaIV GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
NmeAIII GCCGAG 3 cut(s) 850, 1095, 1111
NmuCI GTSAC 3 cut(s) 1922, 2567, 3022
NsiI ATGCAT 4 cut(s) 399, 1132, 2007, 2970
NspI RCATGY 4 cut(s) 1399, 1454, 1775, 2972
NspV TTCGAA 1 cut(s) 2813
PagI TCATGA 1 cut(s) 349
PasI CCCWGGG 1 cut(s) 1558
PctI GAATGC 4 cut(s) 750, 2281, 2798, 2909
PdiI GCCGGC 1 cut(s) 1603
PdmI GAANNNNTTC 2 cut(s) 293, 2355
PfeI GAWTC 8 cut(s) 5, 125, 557, 1154, 1283, 1609, 3051, 3247
PflFI GACNNNGTC 1 cut(s) 3149
PflMI CCANNNNNTGG 2 cut(s) 2292, 2837
PkrI GCNGC 8 cut(s) 51, 119, 238, 527, 2677, 2926, 2929, 3074
PleI GAGTC 2 cut(s) 332, 2433
PmaCI CACGTG 1 cut(s) 2834
PmlI CACGTG 1 cut(s) 2834
PpsI GAGTC 2 cut(s) 332, 2433
Ppu21I YACGTR 1 cut(s) 2834
PpuMI RGGWCCY 1 cut(s) 586
PshBI ATTAAT 1 cut(s) 2738
Psp124BI GAGCTC 2 cut(s) 226, 3123
Psp1406I AACGTT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 586
Psp6I CCWGG 4 cut(s) 1550, 1557, 2986, 3198
PspCI CACGTG 1 cut(s) 2834
PspEI GGTNACC 1 cut(s) 1136
PspFI CCCAGC 3 cut(s) 1016, 1479, 3203
PspGI CCWGG 4 cut(s) 1550, 1557, 2986, 3198
PspN4I GGNNCC 7 cut(s) 450, 587, 588, 1556, 1623, 1657, 3221
PspPI GGNCC 8 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3196, 3219
PspPPI RGGWCCY 1 cut(s) 586
PsrI GAACNNNNNNTAC 2 cut(s) 669, 701
PsuI RGATCY 6 cut(s) 448, 710, 1621, 1655, 1729, 2144
PsyI GACNNNGTC 1 cut(s) 3149
PvuII CAGCTG 3 cut(s) 2112, 2927, 3116
RsaI GTAC 7 cut(s) 661, 678, 950, 955, 1103, 1400, 2024
RsaNI GTAC 7 cut(s) 660, 677, 949, 954, 1102, 1399, 2023
RseI CAYNNNNRTG 5 cut(s) 759, 1400, 1694, 2082, 2127
SacI GAGCTC 2 cut(s) 226, 3123
SatI GCNGC 8 cut(s) 50, 118, 237, 526, 2676, 2925, 2928, 3073
Sau96I GGNCC 8 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3196, 3219
ScaI AGTACT 1 cut(s) 955
SchI GAGTC 2 cut(s) 332, 2434
ScrFI CCNGG 5 cut(s) 18, 1552, 1559, 2988, 3200
SduI GDGCHC 4 cut(s) 226, 2542, 3123, 3287
SfaNI GCATC 7 cut(s) 61, 199, 384, 1117, 1139, 1261, 1468
SfuI TTCGAA 1 cut(s) 2813
SinI GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
SmiMI CAYNNNNRTG 5 cut(s) 759, 1400, 1694, 2082, 2127
SspI AATATT 5 cut(s) 149, 898, 1808, 2252, 2451
SspMI CTAG 4 cut(s) 680, 942, 974, 993
SstI GAGCTC 2 cut(s) 226, 3123
StyD4I CCNGG 5 cut(s) 16, 1550, 1557, 2986, 3198
StyI CCWWGG 4 cut(s) 1429, 1840, 2213, 2721
TaaI ACNGT 9 cut(s) 360, 953, 1151, 1346, 1502, 2573, 2771, 2898, 3128
TaiI ACGT 5 cut(s) 429, 1264, 1747, 2836, 3318
TaqI TCGA 7 cut(s) 74, 409, 1422, 1984, 2813, 3123, 3312
TatI WGTACW 4 cut(s) 676, 953, 1101, 1398
TauI GCSGC 2 cut(s) 239, 528
TfiI GAWTC 8 cut(s) 5, 125, 557, 1154, 1283, 1609, 3051, 3247
TscAI CASTG 4 cut(s) 365, 450, 1795, 2401
TseFI GTSAC 3 cut(s) 1922, 2567, 3022
TseI GCWGC 6 cut(s) 49, 117, 2675, 2924, 2927, 3072
Tsp45I GTSAC 3 cut(s) 1922, 2567, 3022
TspGWI ACGGA 2 cut(s) 677, 3249
TspRI CASTG 4 cut(s) 365, 450, 1795, 2401
Tth111I GACNNNGTC 1 cut(s) 3149
Van91I CCANNNNNTGG 2 cut(s) 2292, 2837
VpaK11BI GGWCC 7 cut(s) 67, 586, 1554, 1580, 2869, 2894, 3219
VspI ATTAAT 1 cut(s) 2738
XapI RAATTY 6 cut(s) 290, 328, 626, 2151, 2400, 2953
XceI RCATGY 4 cut(s) 1399, 1454, 1775, 2972
XmiI GTMKAC 1 cut(s) 3318
XmnI GAANNNNTTC 2 cut(s) 293, 2355
XspI CTAG 4 cut(s) 680, 942, 974, 993
ZraI GACGTC 1 cut(s) 3316
ZrmI AGTACT 1 cut(s) 955
Zsp2I ATGCAT 4 cut(s) 399, 1132, 2007, 2970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.