Rh1BG051800

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010440
Physical Location & Seq
Forward (+)
7201046 .. 7215531
14486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG051800.1

Sequence Viewer

Length: 3165 bp
ATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTTCCTGAAGCTCTTGATAGTGCAGCTCTTTGGATCAAGGGCTTGCCTTTTGTGGAGTCTTTGTCAGGCTATTGGAAATTTTACTTAGCTTCCACTCCTGGAAATGTTCCTTTGAATTTTTACCAGACCGCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGTTCCTTCAAATTGGCAGATGCATGGATTTGATCGGCCTATATACACTAATGTTGAATATCCATTTCCTCTTGATCCACCTTTTGTTCATGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTCCTCCCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATTACTGATTATTGTTATCCTTGTGGGACGGACAAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGTGATGGCTCTTACCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTTCTTTCCAAGCCACAGGTATTTATTGCAGACTATTTCTTCAAATCGAATCTGGCTGACGATTTCTCTTATGCAGACCTACAGGTTGAAGTGAAAATAGATAACTCCCAAGAAACATCCAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAGCTGGTACAGCAGTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCACCAAGGACAGTTCTTGGATTTCGTGATTATTTGCTGGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGCCGAATCTGTACACTCTGGTTGTGATATTGAAAGATACATCTGGCAATATTGTGGACTGTGAATCATGCCTAGTAGGCATACGACAAGTATCAAAAGCCCCTAAGCAGCTGCTTGTTAATGGGCATCCAATAACAATAAGGGGTGTGAACAGGCATGAGCATCATCCACGTCTGGGGAAGACAACTATAGAATCGTGCATGGTTAAGGATTTGGTTTTAATGAAGCAATACAATATCAATGCTGTAAGAAACAGCCATTATCCTCAACATCCCCGCTGGTATGAGCTTTGCGACATGTTTGGCATGTACATGATAGATGAAGCCAATATTGAAACCCATGGGTTCGATTATACTGGACATGTGAAACATCCGACTTTGGAACCCAGTTGGGCAACTGCAATGTTGGACCGTGTGATGGGCATGGTTGAGAGGGACAAAAATCATGCGTGCATTATCTCTTGGTCCTTGGGAAATGAATCTGGATATGGACCTAATCATTCTGCTTTAGCTGGTTGGGTTCGTGGAAAGGATCCCTCACGGCTGTTACATTACGAAGGGGGAGGATCCAGAACCCCATCTACAGATATTATATGCCCCATGTATATGCGTGTTTGGGACATTGTGAAGATTGCGAAAGATCCAAATGAAACACGTCCTCTAATACTGTGCGAGTATTCACATGCAATGGGAAATAGCAATGGAAATATACATGAATATTGGGAAGCAATTGATGGCACATTTGGTCTCCAAGGAGGCTTTATCTGGGAGTGGGTTGATCAGGGCTTACTGAAAGATAGTGCTGATGGTACTAAACACTGGGCATATGGTGGTGACTTTGGTGACGTTCCTAATGATTTAAACTTCTGTTTGAATGGCCTTGTGTGGCCCGATCGAACTCCTCATCCTGCAATGCATGAAGTGAAGTATGTGTACCAGCCAATCAAGGTTTCATTTAGTGAAGGAACACTAAAGATAACAAATACTCACTTTTATGAAACAACAAAAGCATTGGAATTCTGCTGGGCTGCTCATGGAGATGGATGTGAACTTGGATCTGGAAATTTATCCCTTCCCTTGATTGAACCCCAGAAGACTTTTCATATTGAGTTGCAGTCAGCTCCGTGGCATACTCTATGGGCTTCATCATCAGCAGAGGAGTTTTTCTTAACAATAAGTGCTAAGCTTTTGCATGCCACATGCTGGGTTGAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTGCCAAAAGAGAATTTGTTCCTCATGTCATCAAGACTAAAGATACTACATTCCTTAAGGAAATTCTTGGAGATACACTTAAAGTCGGCCAACAGAATGCCTGGGAGATTATACTGAATCTTAAAATGGGAACAGTTGAAAGCTGGAAGGTGGAAGGAGTTCCTTTGATAACAAAAGGCATATTTCCATGTTTTTGGCGAGCACCCACAGATAATGACAAAGGCGGAGGTGCCAGTAGTTATTCGTCCAAGTGGCAAGCTGCTCATATTGATAACCTTTATTATATTACCAAGAGCTGTTCTGTACAGAATATGACTGACCATCTTGTGAAAGTAGCTGTTGTATTTCTCGGTGTTCCAAACAGTGGGGACGGTTCTGGGGTGGAAGACACAAATGCCTTAATTGAAATTGATGTGATTTACACAATCTACGGTTCAGGGGACGTTGTTGTTGAATGCAATGTAAGACCAAGTTTGAACCTTCCGCCTCTGCCACGTGTGGGAGTTGAGTTCCACTTGGAGAAGCCAATAGACCAGATTAAATGGTACGGAAGAGGACCATTTGAATGTTATCCAGACAGAAAAGCAGCTGCTCATGTTGGAGTCTATGAGCAGAAAGTGAGTGATCTGCATGTCCCTTATATAGTTCCTGGGGAATGTTCAGGTAGGGCAGACGTTAGGTGGGTGACATTTCAAAACAAGGATGGTTTGGGAATCTATGCTTCAATATATGGCAGCTCTCCACCCATGCAAATAAATGCAAGTTATTACACCACAGCTGAGCTGGATCGGGCAACACACAATGAAGATCTTATCAGAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTGGCTGGGGATGATAGTTGGTCTCCATGTGTCCATGACAAGTATCTGATTCCTCCTGTACCAAGCTCATTCTCAATTAGGCTCTCTCCAATAACTGCTGCAACCTCTGGCCATGATATCTACAAATCTCAACTTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1054

Amino Acids

118.3

Weight (kDa)

5.41

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 31 - 202 5.4e-54 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 204 - 333 2.2e-13 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 340 - 620 7.2e-98 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 628 - 721 1.9e-18 Beta-galactosidase, domain 4
Bgal_small_N PF02929 752 - 1035 1.8e-72 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2390
AccB7I CCANNNNNTGG 3 cut(s) 339, 2115, 2525
AccI GTMKAC 1 cut(s) 436
AccII CGCG 1 cut(s) 167
AciI CCGC 4 cut(s) 165, 1189, 2385, 2645
AcoI YGGCCR 2 cut(s) 2248, 3130
AcsI RAATTY 8 cut(s) 113, 151, 354, 449, 1928, 1975, 2174, 2223
AcuI CTGAAG 1 cut(s) 63
AcvI CACGTG 1 cut(s) 2657
AfaI GTAC 9 cut(s) 501, 773, 926, 1223, 1723, 1847, 2466, 2708, 3081
AfiI CCNNNNNNNGG 7 cut(s) 339, 1088, 1330, 2115, 2525, 2660, 3028
AflII CTTAAG 1 cut(s) 2216
AflIII ACRYGT 4 cut(s) 1209, 1273, 1565, 2656
AjiI CACGTC 2 cut(s) 1085, 1568
AjnI CCWGG 4 cut(s) 133, 2261, 2809, 3021
AjuI GAANNNNNNNTTGG 2 cut(s) 1846, 1878
AloI GAACNNNNNNTCC 2 cut(s) 1801, 1833
Alw21I GWGCWC 1 cut(s) 2365
Alw26I GTCTC 3 cut(s) 874, 1664, 3048
AoxI GGCC 6 cut(s) 233, 1789, 1799, 2248, 3019, 3130
ApoI RAATTY 8 cut(s) 113, 151, 354, 449, 1928, 1975, 2174, 2223
Asp700I GAANNNNTTC 2 cut(s) 2178, 2319
AspS9I GGNCC 6 cut(s) 1321, 1377, 1403, 1800, 2717, 3019
AsuHPI GGTGA 4 cut(s) 828, 1757, 1766, 2857
AsuII TTCGAA 1 cut(s) 369
AvaII GGWCC 4 cut(s) 1321, 1377, 1403, 2717
BaeI ACNNNNGTAYC 2 cut(s) 763, 796
BalI TGGCCA 1 cut(s) 3132
BamHI GGATCC 2 cut(s) 1444, 1478
BanI GGYRCC 1 cut(s) 2390
BarI GAAGNNNNNNTAC 2 cut(s) 1829, 1861
BbrPI CACGTG 1 cut(s) 2657
BbsI GAAGAC 3 cut(s) 1100, 2012, 2553
Bbv12I GWGCWC 1 cut(s) 2365
BccI CCATC 9 cut(s) 516, 524, 1324, 1498, 1640, 1712, 1946, 2490, 2858
BceAI ACGGC 1 cut(s) 1469
BciT130I CCWGG 4 cut(s) 135, 2263, 2811, 3023
BciVI GTATCC 1 cut(s) 16
BclI TGATCA 2 cut(s) 1690, 3004
BcoDI GTCTC 3 cut(s) 874, 1664, 3048
BfaI CTAG 2 cut(s) 503, 986
BfmI CTRYAG 8 cut(s) 310, 414, 671, 738, 906, 1101, 1494, 3161
BfrI CTTAAG 1 cut(s) 2216
BfuI GTATCC 1 cut(s) 16
BglI GCCNNNNNGGC 1 cut(s) 990
BglII AGATCT 1 cut(s) 2968
BlpI GCTNAGC 2 cut(s) 2094, 2940
Bme1390I CCNGG 4 cut(s) 135, 2263, 2811, 3023
Bme18I GGWCC 4 cut(s) 1321, 1377, 1403, 2717
BmgBI CACGTC 2 cut(s) 1085, 1568
BmgT120I GGNCC 6 cut(s) 1321, 1377, 1403, 1800, 2717, 3019
BmiI GGNNCC 4 cut(s) 1296, 1446, 1480, 2392
BmrFI CCNGG 4 cut(s) 135, 2263, 2811, 3023
BmrI ACTGGG 2 cut(s) 1293, 1741
BmsI GCATC 4 cut(s) 22, 207, 1048, 1084
BmuI ACTGGG 2 cut(s) 1293, 1741
BpiI GAAGAC 3 cut(s) 1100, 2012, 2553
Bpu10I CCTNAGC 1 cut(s) 1017
Bpu1102I GCTNAGC 2 cut(s) 2094, 2940
Bpu14I TTCGAA 1 cut(s) 369
BsaAI YACGTR 1 cut(s) 2657
BsaBI GATNNNNATC 1 cut(s) 552
BsaI GGTCTC 2 cut(s) 1664, 3048
BsaJI CCNNGG 9 cut(s) 20, 839, 893, 1252, 1380, 1663, 2036, 2262, 2810
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 399, 429
Bsc4I CCNNNNNNNGG 7 cut(s) 339, 1088, 1330, 2115, 2525, 2660, 3028
Bse1I ACTGG 8 cut(s) 197, 307, 888, 1273, 1299, 1736, 2155, 2394
Bse3DI GCAATG 5 cut(s) 1320, 1605, 1618, 1830, 2626
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 4 cut(s) 135, 2263, 2811, 3023
BseDI CCNNGG 9 cut(s) 20, 839, 893, 1252, 1380, 1663, 2036, 2262, 2810
BseGI GGATG 9 cut(s) 707, 1039, 1078, 1183, 1282, 1816, 1961, 2869, 3037
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 7 cut(s) 339, 1088, 1330, 2115, 2525, 2660, 3028
BseMI GCAATG 5 cut(s) 1320, 1605, 1618, 1830, 2626
BseMII CTCAG 2 cut(s) 171, 2931
BseNI ACTGG 8 cut(s) 197, 307, 888, 1273, 1299, 1736, 2155, 2394
BseRI GAGGAG 3 cut(s) 1803, 2084, 2994
BseYI CCCAGC 3 cut(s) 1935, 2115, 3026
BsgI GTGCAG 1 cut(s) 78
Bsh1236I CGCG 1 cut(s) 167
Bsh1285I CGRYCG 1 cut(s) 1807
BshFI GGCC 6 cut(s) 235, 1791, 1801, 2250, 3021, 3132
BshNI GGYRCC 1 cut(s) 2390
BsiEI CGRYCG 1 cut(s) 1807
BsiHKAI GWGCWC 1 cut(s) 2365
BslFI GGGAC 6 cut(s) 496, 1361, 1544, 2543, 2615, 2780
BslI CCNNNNNNNGG 7 cut(s) 339, 1088, 1330, 2115, 2525, 2660, 3028
BsmAI GTCTC 3 cut(s) 874, 1664, 3048
BsmFI GGGAC 6 cut(s) 496, 1361, 1544, 2543, 2615, 2780
BsmI GAATGC 3 cut(s) 573, 2263, 2621
BsnI GGCC 6 cut(s) 235, 1791, 1801, 2250, 3021, 3132
Bso31I GGTCTC 2 cut(s) 1664, 3048
Bsp119I TTCGAA 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 2365
Bsp1407I TGTACA 3 cut(s) 924, 1221, 2464
Bsp1720I GCTNAGC 2 cut(s) 2094, 2940
Bsp19I CCATGG 1 cut(s) 1252
BspACI CCGC 4 cut(s) 165, 1189, 2385, 2645
BspANI GGCC 6 cut(s) 235, 1791, 1801, 2250, 3021, 3132
BspCNI CTCAG 2 cut(s) 172, 2932
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 4 cut(s) 1296, 1446, 1480, 2392
BspMAI CTGCAG 1 cut(s) 910
BspT104I TTCGAA 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 2390
BspTI CTTAAG 1 cut(s) 2216
BspTNI GGTCTC 2 cut(s) 1664, 3048
BsrDI GCAATG 5 cut(s) 1320, 1605, 1618, 1830, 2626
BsrGI TGTACA 3 cut(s) 924, 1221, 2464
BsrI ACTGG 8 cut(s) 197, 307, 888, 1273, 1299, 1736, 2155, 2394
BssECI CCNNGG 9 cut(s) 20, 839, 893, 1252, 1380, 1663, 2036, 2262, 2810
BssT1I CCWWGG 6 cut(s) 20, 839, 893, 1252, 1380, 1663
Bst2UI CCWGG 4 cut(s) 135, 2263, 2811, 3023
Bst6I CTCTTC 1 cut(s) 2707
BstAFI CTTAAG 1 cut(s) 2216
BstAUI TGTACA 3 cut(s) 924, 1221, 2464
BstBAI YACGTR 1 cut(s) 2657
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 6 cut(s) 80, 1363, 2106, 2163, 2361, 2418
BstDEI CTNAG 5 cut(s) 121, 180, 1017, 2094, 2940
BstDSI CCRYGG 2 cut(s) 1252, 2036
BstF5I GGATG 9 cut(s) 707, 1039, 1078, 1183, 1282, 1816, 1961, 2869, 3037
BstFNI CGCG 1 cut(s) 167
BstMAI GTCTC 3 cut(s) 874, 1664, 3048
BstMCI CGRYCG 1 cut(s) 1807
BstMWI GCNNNNNNNGC 3 cut(s) 392, 774, 990
BstNI CCWGG 4 cut(s) 135, 2263, 2811, 3023
BstNSI RCATGY 7 cut(s) 1213, 1222, 1277, 1598, 2108, 2115, 2795
BstSCI CCNGG 4 cut(s) 133, 2261, 2809, 3021
BstSFI CTRYAG 8 cut(s) 310, 414, 671, 738, 906, 1101, 1494, 3161
BstUI CGCG 1 cut(s) 167
BstV2I GAAGAC 3 cut(s) 1100, 2012, 2553
BstX2I RGATCY 6 cut(s) 785, 1444, 1478, 1552, 1967, 2968
BstXI CCANNNNNNTGG 2 cut(s) 900, 2355
BstYI RGATCY 6 cut(s) 785, 1444, 1478, 1552, 1967, 2968
BsuI GTATCC 1 cut(s) 16
BsuRI GGCC 6 cut(s) 235, 1791, 1801, 2250, 3021, 3132
BtgI CCRYGG 2 cut(s) 1252, 2036
BtrI CACGTC 2 cut(s) 1085, 1568
BtsCI GGATG 9 cut(s) 707, 1039, 1078, 1183, 1282, 1816, 1961, 2869, 3037
BtsI GCAGTG 1 cut(s) 784
BtsIMutI CAGTG 5 cut(s) 724, 766, 784, 1729, 2530
Cac8I GCNNGC 6 cut(s) 80, 1363, 2106, 2163, 2361, 2418
Cfr13I GGNCC 6 cut(s) 1321, 1377, 1403, 1800, 2717, 3019
Csp6I GTAC 9 cut(s) 500, 772, 925, 1222, 1722, 1846, 2465, 2707, 3080
CviQI GTAC 9 cut(s) 500, 772, 925, 1222, 1722, 1846, 2465, 2707, 3080
DdeI CTNAG 5 cut(s) 121, 180, 1017, 2094, 2940
DraI TTTAAA 1 cut(s) 1773
EaeI YGGCCR 2 cut(s) 2248, 3130
Eam1104I CTCTTC 1 cut(s) 2707
EarI CTCTTC 1 cut(s) 2707
EciI GGCGGA 2 cut(s) 2400, 2634
Eco130I CCWWGG 6 cut(s) 20, 839, 893, 1252, 1380, 1663
Eco31I GGTCTC 2 cut(s) 1664, 3048
Eco32I GATATC 1 cut(s) 3139
Eco47I GGWCC 4 cut(s) 1321, 1377, 1403, 2717
Eco57I CTGAAG 1 cut(s) 63
Eco72I CACGTG 1 cut(s) 2657
EcoRI GAATTC 1 cut(s) 1928
EcoRII CCWGG 4 cut(s) 133, 2261, 2809, 3021
EcoRV GATATC 1 cut(s) 3139
EcoT14I CCWWGG 6 cut(s) 20, 839, 893, 1252, 1380, 1663
EcoT22I ATGCAT 2 cut(s) 222, 1830
ErhI CCWWGG 6 cut(s) 20, 839, 893, 1252, 1380, 1663
FaqI GGGAC 6 cut(s) 496, 1361, 1544, 2543, 2615, 2780
FauI CCCGC 1 cut(s) 1196
FauNDI CATATG 1 cut(s) 1738
FbaI TGATCA 2 cut(s) 1690, 3004
FblI GTMKAC 1 cut(s) 436
FokI GGATG 9 cut(s) 694, 1026, 1065, 1170, 1269, 1803, 1968, 2876, 3044
FspBI CTAG 2 cut(s) 503, 986
GsaI CCCAGC 3 cut(s) 1939, 2119, 3030
HaeIII GGCC 6 cut(s) 235, 1791, 1801, 2250, 3021, 3132
HincII GTYRAC 1 cut(s) 437
HindII GTYRAC 1 cut(s) 437
HindIII AAGCTT 2 cut(s) 824, 2096
HphI GGTGA 4 cut(s) 828, 1757, 1766, 2857
Hpy166II GTNNAC 7 cut(s) 292, 437, 927, 970, 1063, 1846, 1961
Hpy188I TCNGA 4 cut(s) 181, 1287, 2978, 3069
Hpy8I GTNNAC 7 cut(s) 292, 437, 927, 970, 1063, 1846, 1961
HpyAV CCTTC 9 cut(s) 213, 337, 397, 1463, 1868, 1994, 2302, 2309, 2651
HpyCH4IV ACGT 6 cut(s) 1084, 1567, 1758, 2604, 2656, 2835
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 774, 990
HpyF3I CTNAG 5 cut(s) 121, 180, 1017, 2094, 2940
HpySE526I ACGT 6 cut(s) 1084, 1567, 1758, 2604, 2656, 2835
Ksp22I TGATCA 2 cut(s) 1690, 3004
LmnI GCTCC 2 cut(s) 765, 2038
LweI GCATC 4 cut(s) 22, 207, 1048, 1084
MaeI CTAG 2 cut(s) 503, 986
MaeII ACGT 6 cut(s) 1084, 1567, 1758, 2604, 2656, 2835
MaeIII GTNAC 4 cut(s) 1458, 1745, 1754, 2845
MboII GAAGA 9 cut(s) 557, 584, 622, 1105, 1552, 2017, 2558, 2724, 2978
MfeI CAATTG 1 cut(s) 1641
MflI RGATCY 6 cut(s) 785, 1444, 1478, 1552, 1967, 2968
MhlI GDGCHC 1 cut(s) 2365
MlsI TGGCCA 1 cut(s) 3132
MluNI TGGCCA 1 cut(s) 3132
MlyI GAGTC 2 cut(s) 101, 2772
MmeI TCCRAC 4 cut(s) 33, 1299, 1310, 2740
Mox20I TGGCCA 1 cut(s) 3132
Mph1103I ATGCAT 2 cut(s) 222, 1830
MroXI GAANNNNTTC 2 cut(s) 2178, 2319
MscI TGGCCA 1 cut(s) 3132
MslI CAYNNNNRTG 6 cut(s) 582, 1223, 1517, 1905, 1950, 2725
Msp20I TGGCCA 1 cut(s) 3132
MspA1I CMGCKG 4 cut(s) 1024, 1191, 2750, 2939
MspCI CTTAAG 1 cut(s) 2216
MspR9I CCNGG 4 cut(s) 135, 2263, 2811, 3023
MunI CAATTG 1 cut(s) 1641
Mva1269I GAATGC 3 cut(s) 573, 2263, 2621
MvaI CCWGG 4 cut(s) 135, 2263, 2811, 3023
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 3 cut(s) 392, 774, 990
NcoI CCATGG 1 cut(s) 1252
NdeI CATATG 1 cut(s) 1738
NlaIV GGNNCC 4 cut(s) 1296, 1446, 1480, 2392
NmuCI GTSAC 3 cut(s) 1745, 1754, 2845
NsiI ATGCAT 2 cut(s) 222, 1830
NspI RCATGY 7 cut(s) 1213, 1222, 1277, 1598, 2108, 2115, 2795
NspV TTCGAA 1 cut(s) 369
PaeI GCATGC 1 cut(s) 2108
PciI ACATGT 2 cut(s) 1209, 1273
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 3 cut(s) 573, 2263, 2621
PdmI GAANNNNTTC 2 cut(s) 2178, 2319
PflMI CCANNNNNTGG 3 cut(s) 339, 2115, 2525
PfoI TCCNGGA 1 cut(s) 133
Ple19I CGATCG 1 cut(s) 1807
PleI GAGTC 2 cut(s) 100, 2771
PmaCI CACGTG 1 cut(s) 2657
PmlI CACGTG 1 cut(s) 2657
PpsI GAGTC 2 cut(s) 100, 2771
Ppu21I YACGTR 1 cut(s) 2657
PscI ACATGT 2 cut(s) 1209, 1273
Psp6I CCWGG 4 cut(s) 133, 2261, 2809, 3021
PspCI CACGTG 1 cut(s) 2657
PspFI CCCAGC 3 cut(s) 1935, 2115, 3026
PspGI CCWGG 4 cut(s) 133, 2261, 2809, 3021
PspN4I GGNNCC 4 cut(s) 1296, 1446, 1480, 2392
PspPI GGNCC 6 cut(s) 1321, 1377, 1403, 1800, 2717, 3019
PsrI GAACNNNNNNTAC 4 cut(s) 492, 524, 1477, 1509
PstI CTGCAG 1 cut(s) 910
PsuI RGATCY 6 cut(s) 785, 1444, 1478, 1552, 1967, 2968
PvuI CGATCG 1 cut(s) 1807
PvuII CAGCTG 3 cut(s) 1024, 2750, 2939
RsaI GTAC 9 cut(s) 501, 773, 926, 1223, 1723, 1847, 2466, 2708, 3081
RsaNI GTAC 9 cut(s) 500, 772, 925, 1222, 1722, 1846, 2465, 2707, 3080
RseI CAYNNNNRTG 6 cut(s) 582, 1223, 1517, 1905, 1950, 2725
SalI GTCGAC 1 cut(s) 435
Sau96I GGNCC 6 cut(s) 1321, 1377, 1403, 1800, 2717, 3019
SchI GAGTC 2 cut(s) 101, 2772
ScrFI CCNGG 4 cut(s) 135, 2263, 2811, 3023
SduI GDGCHC 1 cut(s) 2365
SfaNI GCATC 4 cut(s) 22, 207, 1048, 1084
SfcI CTRYAG 8 cut(s) 310, 414, 671, 738, 906, 1101, 1494, 3161
SfuI TTCGAA 1 cut(s) 369
SinI GGWCC 4 cut(s) 1321, 1377, 1403, 2717
SmiMI CAYNNNNRTG 6 cut(s) 582, 1223, 1517, 1905, 1950, 2725
SmlI CTYRAG 1 cut(s) 2216
SmoI CTYRAG 1 cut(s) 2216
SphI GCATGC 1 cut(s) 2108
SsiI CCGC 4 cut(s) 165, 1189, 2385, 2645
SspI AATATT 3 cut(s) 964, 1243, 1631
SspMI CTAG 2 cut(s) 503, 986
StyD4I CCNGG 4 cut(s) 133, 2261, 2809, 3021
StyI CCWWGG 6 cut(s) 20, 839, 893, 1252, 1380, 1663
TaiI ACGT 6 cut(s) 1087, 1570, 1761, 2607, 2659, 2838
TaqI TCGA 6 cut(s) 369, 378, 436, 638, 1260, 1807
TatI WGTACW 4 cut(s) 499, 924, 1221, 2464
TscAI CASTG 5 cut(s) 724, 766, 784, 1736, 2530
TseFI GTSAC 3 cut(s) 1745, 1754, 2845
Tsp45I GTSAC 3 cut(s) 1745, 1754, 2845
TspGWI ACGGA 3 cut(s) 500, 2025, 2724
TspRI CASTG 5 cut(s) 724, 766, 784, 1736, 2530
Van91I CCANNNNNTGG 3 cut(s) 339, 2115, 2525
Vha464I CTTAAG 1 cut(s) 2216
VpaK11BI GGWCC 4 cut(s) 1321, 1377, 1403, 2717
XapI RAATTY 8 cut(s) 113, 151, 354, 449, 1928, 1975, 2174, 2223
XceI RCATGY 7 cut(s) 1213, 1222, 1277, 1598, 2108, 2115, 2795
XcmI CCANNNNNNNNNTGG 1 cut(s) 2941
XmiI GTMKAC 1 cut(s) 436
XmnI GAANNNNTTC 2 cut(s) 2178, 2319
XspI CTAG 2 cut(s) 503, 986
Zsp2I ATGCAT 2 cut(s) 222, 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.