Rh1CG062900

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0010440
Physical Location & Seq
Forward (+)
12398647 .. 12461010
62364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG062900.1

Sequence Viewer

Length: 3339 bp
ATGGCTGCTTCTATGATGGGTCAACTGGTTTCTCCCTTGGAGAATGGGCATCATGTGTGGGAGGACCAATCTTTCATCAAGTGGAACAAGAGAGATGCACATGTCACTTTACGTTGCCATGAATCAATTGAAGGATCTCTTAAATACTGGTATGAACGCAACAAAGTGAATTTTATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTTCCTGAAGCTCTTGATAGTGCAGCTCTTTGGATCAAGGGCTTGCCTTTTGTGGAGTCTTTGTCAGGCTATTGGAAATTTTACCTAGCTTCCACTCCTGGAAATGTTCCTTTAAATTTTTACCACACTGCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGTTCCTTCAAATTGGCAGATGCACGGATTTGATCGGCCTATATATACTAATGTTGAATATCCATTTCCTCTTGATCCACCTTTTGTTCATGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTCATCCCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTTGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATTACTGATTATTGTTATCCTTGTGGGACGGACAAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGTGATGGTTCTTACCTTGAAGATCAAGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTTCTTTCCAAGCCACAGGTATTTATTGCAGACTATTTCTTCAAATCGAATCTGGCTGAGGATTTCTCTTATGCAGACCTACAGGTTGAAGTGAAAATAGATAACTCCCAAGAAACATCCAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAGCTGGTACAGCAGTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCACCAAGGACAGTTCTTGGATTTCATGATTATTTGCTGGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGCCGAATCTGTACACTCTGGTTGTGATATTGAAAGATACATCTGGCAATATTGTGGACTGTGAATCATGCCTAGTAGGCATACGACAAGTATCAAAAGCCCCTAAGCAGCTGCTCGTTAATGGGCATCCAATAACAATAAGGGGTGTGAACAGGCATGAGCACCATCCGCGTCTGGGGAAGACAAATATAGAATCGTGCATGGTTAAGGATTTGGTTTTAATGAAGCAATACAATATCAATGCTGTAAGAAACAGCCATTATCCTCAACATCCCCGCTGGTATGAGCTTTGCGACATGTTTGGCATGTACATGATAGATGAAGCCAATATTGAAACCCATGGTTTCGATTATACTGGACATGTGAAACATCCGACTTTGGAACCCAGTTGGGCAACTGCAATGTTGGACCGTGTGATGGGCATGGTTGAGAGGGACAAAAATCATGCGTGCATTATCTCTTGGTCCTTGGGAAATGAATCTGGATATGGACCTAATCATTCTGCTTTAGCTGGTTGGGTTCGTGGAAAGGATCCCTCACGGCTGTTACATTACGAAGGGGGAGGATCCAGAACCCCGTCTACAGATATTATATGCCCCATGTATATGCGTGTTTGGGACATTGTGAAGATTGCGAAAGATCCAAATGAAACACGTCCTCTAATACTGTGCGAGTATTCACATGCAATGGGAAATAGCAATGGAAATATACATGAATATTGGGAAGCAATTGAAGGCACATTTGGTCTCCAAGGAGGCTTTATCTGGGAGTGGGTTGATCAGGGCTTACTGAAAGATAGTGCTGATGGTACTAAACACTGGGCATATGGTGGTGACTTTGGTGACGTTCCTAATGATTTAAACTTCTGTTTGAATGGCCTTGTGTGGCCCAATCGAACTCCTCATCCTGCAATGCATGAAGTGAAGTATGTATACCAACCAATCAAGGTTTCATTTAGTGAAGGAACACTAAAGATAACAAATACTCACTTTTACGAAACAACAAAAGCATTGGAATTCAGCTGGGCTGCTCATGGAGATGGATGTGAACTTGGATCTGGAAATTTATCCCTTCCCTTGATTGAACCCCAGAAGACTTTTCATATTGAGTTGCAGTCAGCTCCGTGGCATACTCTATGGGCTTCATCATCAGCAGAGGAGTTTTTCTTAACAATAAGTGCTAAGCTTTTGCATGCCACATGCTGGGTTGAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTGCCAAAAGAGAATTTGTTCCTCATGTCATCAAGACTAAAGATACTACATTCCTTAAGGAAATTCTTGGAGATACACTTAAAGTTGGCCAACAGAATGCCTGGGAGATTATACTGAATCTTAAATTGGGAACAGTTGAAAGCTGGAAGGTGGAAGGAGTTCCTTTGATGACAAAAGGCATATTTCCATGTTTTTGGCGAGCACCCACAGATAATGACAAAGGCGGAGGTGCCAGTAGTTATTTGTCCAAGTGGCAAGCTGCGCATATTGATAACCTTCATTATATTACCAAGAGCTGTTCTGTACAGAATATGACTGACCATCTTGTGAAAGTAGCTGTTGTTTTTCTCGGTGTTCCAAACAGTGGGGACGGTTCTGGGGTGGAAGACACAAATGCCTTAATTGAAATTGATGTGATTTACACAATCTACGGTTCAGGGGATGTTGTTGTTGAATGCAATGTAAGACCAAGTTCGAACCTTCCGCCTCTGCCACGTGTGGGAGTTGAGTTCCACCTGGAGAAGCCAATAGACCAGATTAAATGGTACGGAAGAGGACCATTTGAATGTTATCCAGACAGAAAAGCAGCTGCTCATGTTGGAGTCTATGAGCAGAAAGTGAGTGATCTGCATGTCCCTTATATAGTTCCTGGGGAATGTTCAGGTAGGGCAGACGTTAGGTGGGTGACATTTCAAAACAAGGACGGTTTGGGAATCTATGCTTCAATATATGGCAGCTCTCCACCCATGCAAATAAGTGCAAGTTATTATACCACAGCTGAGCTGGATCGGGCAACACACAATGAAGATCTTATCAGAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTGGCCGGGGATGATAGTTGGTCTCCATGTGTCCATGACAAGTATCTGATTCCTCCTGTTCCAAGCTCATTCTCAATTAGGCTCTCGCCAATAACTCCTGCAACCTCTGGCCATGATATCTACAAATCTCAACTTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

1112

Amino Acids

125.17

Weight (kDa)

5.58

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 260 4.3e-54 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 262 - 391 5.2e-13 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 398 - 678 7.8e-97 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 686 - 779 1.3e-18 Beta-galactosidase, domain 4
Bgal_small_N PF02929 810 - 1093 2.2e-72 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2598
AccB1I GGYRCC 1 cut(s) 2564
AccB7I CCANNNNNTGG 3 cut(s) 513, 2289, 2699
AccI GTMKAC 3 cut(s) 610, 1667, 2019
AccII CGCG 1 cut(s) 1258
AciI CCGC 4 cut(s) 1256, 1363, 2559, 2819
AcoI YGGCCR 3 cut(s) 2422, 3198, 3304
AcsI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2102, 2149, 2348, 2397
AcuI CTGAAG 1 cut(s) 237
AcvI CACGTG 1 cut(s) 2831
AfaI GTAC 7 cut(s) 675, 947, 1100, 1397, 1897, 2640, 2882
AfiI CCNNNNNNNGG 7 cut(s) 513, 1262, 1504, 2289, 2699, 2834, 3202
AflII CTTAAG 1 cut(s) 2390
AflIII ACRYGT 5 cut(s) 100, 1383, 1447, 1739, 2830
AjiI CACGTC 1 cut(s) 1742
AjnI CCWGG 5 cut(s) 307, 2435, 2850, 2983, 3195
AjuI GAANNNNNNNTTGG 6 cut(s) 1812, 1844, 2020, 2052, 2444, 2476
AloI GAACNNNNNNTCC 4 cut(s) 691, 723, 1975, 2007
Alw21I GWGCWC 2 cut(s) 1251, 2539
Alw26I GTCTC 3 cut(s) 1048, 1838, 3222
AoxI GGCC 7 cut(s) 407, 1963, 1973, 2422, 3193, 3198, 3304
ApoI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2102, 2149, 2348, 2397
Asp700I GAANNNNTTC 2 cut(s) 2352, 2493
AspLEI GCGC 1 cut(s) 2599
AspS9I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2891, 3193
AsuC2I CCSGG 1 cut(s) 3202
AsuHPI GGTGA 4 cut(s) 1002, 1931, 1940, 3031
AsuII TTCGAA 1 cut(s) 2810
AvaII GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2891
BaeI ACNNNNGTAYC 2 cut(s) 937, 970
BalI TGGCCA 2 cut(s) 2424, 3306
BamHI GGATCC 2 cut(s) 1618, 1652
BanI GGYRCC 1 cut(s) 2564
BarI GAAGNNNNNNTAC 2 cut(s) 2003, 2035
BbrPI CACGTG 1 cut(s) 2831
BbsI GAAGAC 3 cut(s) 1274, 2186, 2727
Bbv12I GWGCWC 2 cut(s) 1251, 2539
BbvCI CCTCAGC 1 cut(s) 822
BccI CCATC 8 cut(s) 10, 690, 698, 1260, 1498, 1886, 2120, 2664
BceAI ACGGC 1 cut(s) 1643
BciT130I CCWGG 5 cut(s) 309, 2437, 2852, 2985, 3197
BciVI GTATCC 1 cut(s) 190
BclI TGATCA 2 cut(s) 1864, 3178
BcnI CCSGG 1 cut(s) 3202
BcoDI GTCTC 3 cut(s) 1048, 1838, 3222
BfaI CTAG 3 cut(s) 296, 677, 1160
BfmI CTRYAG 7 cut(s) 484, 588, 845, 912, 1080, 1668, 3335
BfrI CTTAAG 1 cut(s) 2390
BfuI GTATCC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 1164
BglII AGATCT 1 cut(s) 3142
BlpI GCTNAGC 2 cut(s) 2268, 3114
Bme1390I CCNGG 6 cut(s) 309, 2437, 2852, 2985, 3197, 3202
Bme18I GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2891
BmgBI CACGTC 1 cut(s) 1742
BmgT120I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2891, 3193
BmiI GGNNCC 4 cut(s) 1470, 1620, 1654, 2566
BmrFI CCNGG 6 cut(s) 309, 2437, 2852, 2985, 3197, 3202
BmrI ACTGGG 2 cut(s) 1467, 1915
BmsI GCATC 5 cut(s) 58, 85, 196, 381, 1222
BmuI ACTGGG 2 cut(s) 1467, 1915
BpiI GAAGAC 3 cut(s) 1274, 2186, 2727
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 1 cut(s) 2873
Bpu10I CCTNAGC 2 cut(s) 822, 1191
Bpu1102I GCTNAGC 2 cut(s) 2268, 3114
Bpu14I TTCGAA 1 cut(s) 2810
BpuMI CCSGG 1 cut(s) 3202
BsaAI YACGTR 1 cut(s) 2831
BsaBI GATNNNNATC 1 cut(s) 726
BsaI GGTCTC 2 cut(s) 1838, 3222
BsaXI ACNNNNNCTCC 6 cut(s) 257, 287, 573, 603, 691, 721
Bsc4I CCNNNNNNNGG 7 cut(s) 513, 1262, 1504, 2289, 2699, 2834, 3202
Bse3DI GCAATG 5 cut(s) 1494, 1779, 1792, 2004, 2800
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 5 cut(s) 309, 2437, 2852, 2985, 3197
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 7 cut(s) 513, 1262, 1504, 2289, 2699, 2834, 3202
BseMI GCAATG 5 cut(s) 1494, 1779, 1792, 2004, 2800
BseMII CTCAG 3 cut(s) 345, 813, 3105
BseRI GAGGAG 3 cut(s) 1977, 2258, 3168
BseYI CCCAGC 2 cut(s) 2109, 2289
BsgI GTGCAG 2 cut(s) 174, 252
Bsh1236I CGCG 1 cut(s) 1258
BshFI GGCC 7 cut(s) 409, 1965, 1975, 2424, 3195, 3200, 3306
BshNI GGYRCC 1 cut(s) 2564
BsiHKAI GWGCWC 2 cut(s) 1251, 2539
BsiSI CCGG 1 cut(s) 3201
BslFI GGGAC 5 cut(s) 670, 1535, 1718, 2717, 2954
BslI CCNNNNNNNGG 7 cut(s) 513, 1262, 1504, 2289, 2699, 2834, 3202
BsmAI GTCTC 3 cut(s) 1048, 1838, 3222
BsmFI GGGAC 5 cut(s) 670, 1535, 1718, 2717, 2954
BsmI GAATGC 3 cut(s) 747, 2437, 2795
BsnI GGCC 7 cut(s) 409, 1965, 1975, 2424, 3195, 3200, 3306
Bso31I GGTCTC 2 cut(s) 1838, 3222
Bsp119I TTCGAA 1 cut(s) 2810
Bsp1286I GDGCHC 2 cut(s) 1251, 2539
Bsp1407I TGTACA 3 cut(s) 1098, 1395, 2638
Bsp1720I GCTNAGC 2 cut(s) 2268, 3114
Bsp19I CCATGG 1 cut(s) 1426
BspACI CCGC 4 cut(s) 1256, 1363, 2559, 2819
BspANI GGCC 7 cut(s) 409, 1965, 1975, 2424, 3195, 3200, 3306
BspCNI CTCAG 3 cut(s) 346, 814, 3106
BspFNI CGCG 1 cut(s) 1258
BspHI TCATGA 1 cut(s) 1033
BspLI GGNNCC 4 cut(s) 1470, 1620, 1654, 2566
BspMAI CTGCAG 1 cut(s) 1084
BspT104I TTCGAA 1 cut(s) 2810
BspT107I GGYRCC 1 cut(s) 2564
BspTI CTTAAG 1 cut(s) 2390
BspTNI GGTCTC 2 cut(s) 1838, 3222
BsrDI GCAATG 5 cut(s) 1494, 1779, 1792, 2004, 2800
BsrGI TGTACA 3 cut(s) 1098, 1395, 2638
BssNAI GTATAC 1 cut(s) 2020
BssT1I CCWWGG 7 cut(s) 36, 194, 1013, 1067, 1426, 1554, 1837
Bst1107I GTATAC 1 cut(s) 2020
Bst2UI CCWGG 5 cut(s) 309, 2437, 2852, 2985, 3197
Bst6I CTCTTC 1 cut(s) 2881
BstAFI CTTAAG 1 cut(s) 2390
BstAUI TGTACA 3 cut(s) 1098, 1395, 2638
BstBAI YACGTR 1 cut(s) 2831
BstBI TTCGAA 1 cut(s) 2810
BstC8I GCNNGC 6 cut(s) 254, 1537, 2280, 2337, 2535, 2592
BstDEI CTNAG 5 cut(s) 354, 822, 1191, 2268, 3114
BstDSI CCRYGG 2 cut(s) 1426, 2210
BstFNI CGCG 1 cut(s) 1258
BstHHI GCGC 1 cut(s) 2599
BstMAI GTCTC 3 cut(s) 1048, 1838, 3222
BstMWI GCNNNNNNNGC 5 cut(s) 566, 948, 1164, 1255, 2596
BstNI CCWGG 5 cut(s) 309, 2437, 2852, 2985, 3197
BstNSI RCATGY 8 cut(s) 104, 1387, 1396, 1451, 1772, 2282, 2289, 2969
BstSCI CCNGG 6 cut(s) 307, 2435, 2850, 2983, 3195, 3200
BstSFI CTRYAG 7 cut(s) 484, 588, 845, 912, 1080, 1668, 3335
BstUI CGCG 1 cut(s) 1258
BstV2I GAAGAC 3 cut(s) 1274, 2186, 2727
BstX2I RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3142
BstXI CCANNNNNNTGG 2 cut(s) 1074, 2529
BstYI RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3142
BstZ17I GTATAC 1 cut(s) 2020
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 7 cut(s) 409, 1965, 1975, 2424, 3195, 3200, 3306
BtgI CCRYGG 2 cut(s) 1426, 2210
BtrI CACGTC 1 cut(s) 1742
BtsI GCAGTG 2 cut(s) 336, 958
BtsIMutI CAGTG 6 cut(s) 336, 898, 940, 958, 1903, 2704
Cac8I GCNNGC 6 cut(s) 254, 1537, 2280, 2337, 2535, 2592
CciI TCATGA 1 cut(s) 1033
CfoI GCGC 1 cut(s) 2599
Cfr13I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2891, 3193
CseI GACGC 1 cut(s) 1247
Csp6I GTAC 7 cut(s) 674, 946, 1099, 1396, 1896, 2639, 2881
CviQI GTAC 7 cut(s) 674, 946, 1099, 1396, 1896, 2639, 2881
DdeI CTNAG 5 cut(s) 354, 822, 1191, 2268, 3114
DraI TTTAAA 2 cut(s) 324, 1947
EaeI YGGCCR 3 cut(s) 2422, 3198, 3304
Eam1104I CTCTTC 1 cut(s) 2881
EarI CTCTTC 1 cut(s) 2881
EciI GGCGGA 2 cut(s) 2574, 2808
Eco130I CCWWGG 7 cut(s) 36, 194, 1013, 1067, 1426, 1554, 1837
Eco31I GGTCTC 2 cut(s) 1838, 3222
Eco32I GATATC 1 cut(s) 3313
Eco47I GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2891
Eco57I CTGAAG 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 2831
EcoRI GAATTC 1 cut(s) 2102
EcoRII CCWGG 5 cut(s) 307, 2435, 2850, 2983, 3195
EcoRV GATATC 1 cut(s) 3313
EcoT14I CCWWGG 7 cut(s) 36, 194, 1013, 1067, 1426, 1554, 1837
EcoT22I ATGCAT 1 cut(s) 2004
ErhI CCWWGG 7 cut(s) 36, 194, 1013, 1067, 1426, 1554, 1837
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 5 cut(s) 670, 1535, 1718, 2717, 2954
FauI CCCGC 1 cut(s) 1370
FauNDI CATATG 1 cut(s) 1912
FbaI TGATCA 2 cut(s) 1864, 3178
FblI GTMKAC 3 cut(s) 610, 1667, 2019
FspBI CTAG 3 cut(s) 296, 677, 1160
FspI TGCGCA 1 cut(s) 2598
GlaI GCGC 1 cut(s) 2598
GsaI CCCAGC 2 cut(s) 2113, 2293
GsuI CTGGAG 1 cut(s) 2873
HaeIII GGCC 7 cut(s) 409, 1965, 1975, 2424, 3195, 3200, 3306
HapII CCGG 1 cut(s) 3201
HgaI GACGC 1 cut(s) 1247
HhaI GCGC 1 cut(s) 2599
Hin6I GCGC 1 cut(s) 2597
HinP1I GCGC 1 cut(s) 2597
HincII GTYRAC 2 cut(s) 23, 611
HindII GTYRAC 2 cut(s) 23, 611
HindIII AAGCTT 2 cut(s) 998, 2270
HpaII CCGG 1 cut(s) 3201
HphI GGTGA 4 cut(s) 1002, 1931, 1940, 3031
Hpy166II GTNNAC 9 cut(s) 23, 466, 611, 1101, 1144, 1237, 1668, 2020, 2135
Hpy188I TCNGA 4 cut(s) 355, 1461, 3152, 3243
Hpy8I GTNNAC 9 cut(s) 23, 466, 611, 1101, 1144, 1237, 1668, 2020, 2135
HpyCH4IV ACGT 5 cut(s) 112, 1741, 1932, 2830, 3009
HpyF10VI GCNNNNNNNGC 5 cut(s) 566, 948, 1164, 1255, 2596
HpyF3I CTNAG 5 cut(s) 354, 822, 1191, 2268, 3114
HpySE526I ACGT 5 cut(s) 112, 1741, 1932, 2830, 3009
HspAI GCGC 1 cut(s) 2597
Ksp22I TGATCA 2 cut(s) 1864, 3178
LmnI GCTCC 2 cut(s) 939, 2212
LweI GCATC 5 cut(s) 58, 85, 196, 381, 1222
MaeI CTAG 3 cut(s) 296, 677, 1160
MaeII ACGT 5 cut(s) 112, 1741, 1932, 2830, 3009
MaeIII GTNAC 5 cut(s) 103, 1632, 1919, 1928, 3019
MboII GAAGA 9 cut(s) 731, 758, 796, 1279, 1726, 2191, 2732, 2898, 3152
MfeI CAATTG 2 cut(s) 126, 1815
MflI RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3142
MhlI GDGCHC 2 cut(s) 1251, 2539
MlsI TGGCCA 2 cut(s) 2424, 3306
MluNI TGGCCA 2 cut(s) 2424, 3306
MlyI GAGTC 2 cut(s) 275, 2946
MmeI TCCRAC 4 cut(s) 207, 1473, 1484, 2914
Mox20I TGGCCA 2 cut(s) 2424, 3306
Mph1103I ATGCAT 1 cut(s) 2004
MroXI GAANNNNTTC 2 cut(s) 2352, 2493
MscI TGGCCA 2 cut(s) 2424, 3306
MslI CAYNNNNRTG 5 cut(s) 756, 1397, 1691, 2124, 2899
Msp20I TGGCCA 2 cut(s) 2424, 3306
MspA1I CMGCKG 5 cut(s) 1198, 1365, 2109, 2924, 3113
MspCI CTTAAG 1 cut(s) 2390
MspI CCGG 1 cut(s) 3201
MspR9I CCNGG 6 cut(s) 309, 2437, 2852, 2985, 3197, 3202
MunI CAATTG 2 cut(s) 126, 1815
Mva1269I GAATGC 3 cut(s) 747, 2437, 2795
MvaI CCWGG 5 cut(s) 309, 2437, 2852, 2985, 3197
MvnI CGCG 1 cut(s) 1258
MwoI GCNNNNNNNGC 5 cut(s) 566, 948, 1164, 1255, 2596
NciI CCSGG 1 cut(s) 3202
NcoI CCATGG 1 cut(s) 1426
NdeI CATATG 1 cut(s) 1912
NlaIV GGNNCC 4 cut(s) 1470, 1620, 1654, 2566
NmuCI GTSAC 4 cut(s) 103, 1919, 1928, 3019
NsbI TGCGCA 1 cut(s) 2598
NsiI ATGCAT 1 cut(s) 2004
NspI RCATGY 8 cut(s) 104, 1387, 1396, 1451, 1772, 2282, 2289, 2969
NspV TTCGAA 1 cut(s) 2810
PaeI GCATGC 1 cut(s) 2282
PagI TCATGA 1 cut(s) 1033
PciI ACATGT 3 cut(s) 100, 1383, 1447
PctI GAATGC 3 cut(s) 747, 2437, 2795
PdmI GAANNNNTTC 2 cut(s) 2352, 2493
PflMI CCANNNNNTGG 3 cut(s) 513, 2289, 2699
PfoI TCCNGGA 1 cut(s) 307
PleI GAGTC 2 cut(s) 274, 2945
PmaCI CACGTG 1 cut(s) 2831
PmlI CACGTG 1 cut(s) 2831
PpsI GAGTC 2 cut(s) 274, 2945
Ppu21I YACGTR 1 cut(s) 2831
PscI ACATGT 3 cut(s) 100, 1383, 1447
Psp6I CCWGG 5 cut(s) 307, 2435, 2850, 2983, 3195
PspCI CACGTG 1 cut(s) 2831
PspFI CCCAGC 2 cut(s) 2109, 2289
PspGI CCWGG 5 cut(s) 307, 2435, 2850, 2983, 3195
PspN4I GGNNCC 4 cut(s) 1470, 1620, 1654, 2566
PspPI GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2891, 3193
PsrI GAACNNNNNNTAC 4 cut(s) 666, 698, 1651, 1683
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3142
PvuII CAGCTG 4 cut(s) 1198, 2109, 2924, 3113
RsaI GTAC 7 cut(s) 675, 947, 1100, 1397, 1897, 2640, 2882
RsaNI GTAC 7 cut(s) 674, 946, 1099, 1396, 1896, 2639, 2881
RseI CAYNNNNRTG 5 cut(s) 756, 1397, 1691, 2124, 2899
SalI GTCGAC 1 cut(s) 609
Sau96I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2891, 3193
SchI GAGTC 2 cut(s) 275, 2946
ScrFI CCNGG 6 cut(s) 309, 2437, 2852, 2985, 3197, 3202
SduI GDGCHC 2 cut(s) 1251, 2539
SfaNI GCATC 5 cut(s) 58, 85, 196, 381, 1222
SfcI CTRYAG 7 cut(s) 484, 588, 845, 912, 1080, 1668, 3335
SfuI TTCGAA 1 cut(s) 2810
SinI GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2891
SmiMI CAYNNNNRTG 5 cut(s) 756, 1397, 1691, 2124, 2899
SmlI CTYRAG 1 cut(s) 2390
SmoI CTYRAG 1 cut(s) 2390
SphI GCATGC 1 cut(s) 2282
SsiI CCGC 4 cut(s) 1256, 1363, 2559, 2819
SspI AATATT 3 cut(s) 1138, 1417, 1805
SspMI CTAG 3 cut(s) 296, 677, 1160
StyD4I CCNGG 6 cut(s) 307, 2435, 2850, 2983, 3195, 3200
StyI CCWWGG 7 cut(s) 36, 194, 1013, 1067, 1426, 1554, 1837
TaiI ACGT 5 cut(s) 115, 1744, 1935, 2833, 3012
TaqI TCGA 6 cut(s) 552, 610, 812, 1434, 1981, 2810
TatI WGTACW 4 cut(s) 673, 1098, 1395, 2638
TscAI CASTG 6 cut(s) 343, 898, 940, 958, 1910, 2704
TseFI GTSAC 4 cut(s) 103, 1919, 1928, 3019
Tsp45I GTSAC 4 cut(s) 103, 1919, 1928, 3019
TspGWI ACGGA 4 cut(s) 411, 674, 2199, 2898
TspRI CASTG 6 cut(s) 343, 898, 940, 958, 1910, 2704
Van91I CCANNNNNTGG 3 cut(s) 513, 2289, 2699
Vha464I CTTAAG 1 cut(s) 2390
VpaK11BI GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2891
XapI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2102, 2149, 2348, 2397
XceI RCATGY 8 cut(s) 104, 1387, 1396, 1451, 1772, 2282, 2289, 2969
XcmI CCANNNNNNNNNTGG 1 cut(s) 3115
XmiI GTMKAC 3 cut(s) 610, 1667, 2019
XmnI GAANNNNTTC 2 cut(s) 2352, 2493
XspI CTAG 3 cut(s) 296, 677, 1160
Zsp2I ATGCAT 1 cut(s) 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.