AT3G55620

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005509
Physical Location & Seq
Forward (+)
20634057 .. 20636555
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55620.1

Sequence Viewer

Length: 738 bp
ATGGCGACTCGTCTTCAGTTTGAGAACAACTGCGAAGTTGGTGTCTTTTCCAAACTCACTAATGCCTATTGCTTAGTCGCTATAGGAGGCTCTGAGAACTTTTACAGTGCTTTTGAGTCGGAGTTAGCAGATGTTATCCCTATTGTTAAGACCTCTATTGGAGGAACACGAATTATTGGGCGTCTTTGTGCTGGAAACAAGAATGGGCTTCTTGTACCTCATACAACAACTGACCAAGAGCTTCAACACTTAAGGAACAGTCTACCTGATCAAGTTGTGGTCCAGCGAATTGATGAACGTCTGTCTGCTCTTGGAAATTGCATTGCCTGCAATGACTATGTAGCTCTTGCCCACACCGATCTTGATAAGGAAACCGAGGAAATAATAGCGGATGTACTTGGCGTTGAAGTTTTCCGTCAGACAATTGCAGGCAACATTCTCGTGGGCAGTTACTGTGCCCTATCCAACAAAGGTGGAATGGTCCATCCTCACACCTCAGTGGAAGACTTGGAAGAACTCTCAACACTTCTTCAAGTCCCTCTCGTTGCAGGAACCGTGAACCGTGGTAGTGAAGTTATCGCTGCGGGTATGACCGTTAATGATTGGACCTCTTTCTGCGGTTCAGACACAACTGCGACTGAGCTCTCTGTCATAGACAGCATCTTCAAGCTAAGGGAAGCCCAACCCAGCTCTATCGTCGATGAGATGAGGAAGTCATTGATCGATACCTATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000460 GO:0000470 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010154 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033750 GO:0033993 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042221 GO:0042254 GO:0042273 GO:0042886 GO:0043021 GO:0043023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050896 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051716 GO:0061458 GO:0065003 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071426 GO:0071428 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097305 GO:0097306 GO:1901360 GO:1901700 GO:1901701 GO:1902626 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
eIF-6

Protein Analysis

245

Amino Acids

26.48

Weight (kDa)

4.63

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-6 PF01912 4 - 203 3.5e-81 eIF-6 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 262
AciI CCGC 3 cut(s) 389, 584, 618
AcyI GRCGYC 1 cut(s) 181
AfaI GTAC 2 cut(s) 216, 396
AflII CTTAAG 1 cut(s) 250
AgsI TTSAA 4 cut(s) 245, 407, 533, 667
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 5 cut(s) 241, 344, 643, 670, 690
AluI AGCT 5 cut(s) 241, 344, 643, 670, 690
Alw21I GWGCWC 1 cut(s) 645
AlwNI CAGNNNCTG 1 cut(s) 453
ApeKI GCWGC 1 cut(s) 581
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
AspS9I GGNCC 3 cut(s) 280, 481, 606
AvaII GGWCC 3 cut(s) 280, 481, 606
BaeGI GKGCMC 1 cut(s) 460
BaeI ACNNNNGTAYC 1 cut(s) 717
BanII GRGCYC 1 cut(s) 645
BauI CACGAG 1 cut(s) 440
BbsI GAAGAC 2 cut(s) 5, 510
Bbv12I GWGCWC 1 cut(s) 645
BbvI GCAGC 1 cut(s) 568
BccI CCATC 1 cut(s) 492
BcgI CGANNNNNNTGC 2 cut(s) 421, 455
BclI TGATCA 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 81
BfrI CTTAAG 1 cut(s) 250
BisI GCNGC 1 cut(s) 582
BlsI GCNGC 1 cut(s) 583
Bme18I GGWCC 3 cut(s) 280, 481, 606
BmgT120I GGNCC 3 cut(s) 280, 481, 606
BmiI GGNNCC 1 cut(s) 553
BmsI GCATC 1 cut(s) 669
BpiI GAAGAC 2 cut(s) 5, 510
Bpu10I CCTNAGC 1 cut(s) 671
Bsa29I ATCGAT 1 cut(s) 723
BsaHI GRCGYC 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 375, 562
Bse3DI GCAATG 2 cut(s) 321, 337
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 2 cut(s) 375, 562
BseGI GGATG 2 cut(s) 397, 484
BseMI GCAATG 2 cut(s) 321, 337
BseMII CTCAG 3 cut(s) 84, 510, 630
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 1 cut(s) 568
BseYI CCCAGC 1 cut(s) 686
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 645
BslFI GGGAC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 460, 645
Bsp143I GATC 3 cut(s) 268, 358, 720
BspACI CCGC 3 cut(s) 389, 584, 618
BspCNI CTCAG 3 cut(s) 85, 509, 631
BspDI ATCGAT 1 cut(s) 723
BspLI GGNNCC 1 cut(s) 553
BspTI CTTAAG 1 cut(s) 250
BsrDI GCAATG 2 cut(s) 321, 337
BssECI CCNNGG 2 cut(s) 375, 562
BssMI GATC 3 cut(s) 268, 358, 720
BssNI GRCGYC 1 cut(s) 181
BssSI CACGAG 1 cut(s) 440
Bst2BI CACGAG 1 cut(s) 440
Bst4CI ACNGT 6 cut(s) 107, 260, 455, 556, 563, 595
BstACI GRCGYC 1 cut(s) 181
BstAFI CTTAAG 1 cut(s) 250
BstAPI GCANNNNNTGC 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 328, 430
BstDEI CTNAG 5 cut(s) 73, 93, 496, 639, 671
BstDSI CCRYGG 1 cut(s) 562
BstF5I GGATG 2 cut(s) 397, 484
BstKTI GATC 3 cut(s) 271, 361, 723
BstMBI GATC 3 cut(s) 268, 358, 720
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstSFI CTRYAG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 1 cut(s) 568
BstV2I GAAGAC 2 cut(s) 5, 510
Bsu15I ATCGAT 1 cut(s) 723
BsuTUI ATCGAT 1 cut(s) 723
BtgI CCRYGG 1 cut(s) 562
BtsCI GGATG 2 cut(s) 397, 484
BtsIMutI CAGTG 2 cut(s) 112, 504
Cac8I GCNNGC 2 cut(s) 328, 430
CaiI CAGNNNCTG 1 cut(s) 453
Cfr13I GGNCC 3 cut(s) 280, 481, 606
ClaI ATCGAT 1 cut(s) 723
CseI GACGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 215, 395
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviJI RGCY 8 cut(s) 90, 208, 241, 344, 643, 670, 680, 690
CviKI_1 RGCY 8 cut(s) 90, 208, 241, 344, 643, 670, 680, 690
CviQI GTAC 2 cut(s) 215, 395
DdeI CTNAG 5 cut(s) 73, 93, 496, 639, 671
DpnI GATC 3 cut(s) 270, 360, 722
DpnII GATC 3 cut(s) 268, 358, 720
Ecl136II GAGCTC 1 cut(s) 643
Eco24I GRGCYC 1 cut(s) 645
Eco47I GGWCC 3 cut(s) 280, 481, 606
Eco53kI GAGCTC 1 cut(s) 643
EcoICRI GAGCTC 1 cut(s) 643
EcoT38I GRGCYC 1 cut(s) 645
FaiI YATR 6 cut(s) 83, 222, 339, 590, 653, 732
FaqI GGGAC 1 cut(s) 521
FauI CCCGC 1 cut(s) 577
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 582
FokI GGATG 2 cut(s) 404, 471
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 1 cut(s) 582
GluI GCNGC 1 cut(s) 582
GsaI CCCAGC 1 cut(s) 690
HgaI GACGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 181
HinfI GANTC 2 cut(s) 7, 116
Hpy166II GTNNAC 2 cut(s) 263, 559
Hpy188I TCNGA 4 cut(s) 94, 121, 420, 625
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 2 cut(s) 263, 559
Hpy99I CGWCG 1 cut(s) 701
HpyCH4III ACNGT 6 cut(s) 107, 260, 455, 556, 563, 595
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 4 cut(s) 321, 330, 428, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpyF3I CTNAG 5 cut(s) 73, 93, 496, 639, 671
HpySE526I ACGT 1 cut(s) 298
Hsp92I GRCGYC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 268, 358, 720
LpnPI CCDG 7 cut(s) 177, 279, 296, 340, 414, 534, 700
Lsp1109I GCAGC 1 cut(s) 568
LweI GCATC 1 cut(s) 669
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 449
MalI GATC 3 cut(s) 270, 360, 722
MboI GATC 3 cut(s) 268, 358, 720
MboII GAAGA 5 cut(s) 5, 515, 521, 524, 655
MfeI CAATTG 1 cut(s) 423
MhlI GDGCHC 2 cut(s) 460, 645
MluCI AATT 4 cut(s) 171, 288, 316, 423
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 2 cut(s) 99, 489
MseI TTAA 4 cut(s) 147, 251, 597, 736
MslI CAYNNNNRTG 2 cut(s) 440, 497
MspCI CTTAAG 1 cut(s) 250
MunI CAATTG 1 cut(s) 423
MwoI GCNNNNNNNGC 1 cut(s) 327
NdeII GATC 3 cut(s) 268, 358, 720
NlaIV GGNNCC 1 cut(s) 553
OliI CACNNNNGTG 1 cut(s) 497
PkrI GCNGC 1 cut(s) 583
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 686
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 3 cut(s) 280, 481, 606
PstNI CAGNNNCTG 1 cut(s) 453
RsaI GTAC 2 cut(s) 216, 396
RsaNI GTAC 2 cut(s) 215, 395
RseI CAYNNNNRTG 2 cut(s) 440, 497
SacI GAGCTC 1 cut(s) 645
SaqAI TTAA 4 cut(s) 147, 251, 597, 736
SatI GCNGC 1 cut(s) 582
Sau3AI GATC 3 cut(s) 268, 358, 720
Sau96I GGNCC 3 cut(s) 280, 481, 606
SchI GAGTC 1 cut(s) 125
SduI GDGCHC 2 cut(s) 460, 645
SfaNI GCATC 1 cut(s) 669
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 3 cut(s) 280, 481, 606
SmiMI CAYNNNNRTG 2 cut(s) 440, 497
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 4 cut(s) 171, 288, 316, 423
SsiI CCGC 3 cut(s) 389, 584, 618
SstI GAGCTC 1 cut(s) 645
TaaI ACNGT 6 cut(s) 107, 260, 455, 556, 563, 595
TaiI ACGT 1 cut(s) 301
TaqI TCGA 2 cut(s) 699, 723
TasI AATT 4 cut(s) 171, 288, 316, 423
TatI WGTACW 1 cut(s) 394
Tru1I TTAA 4 cut(s) 147, 251, 597, 736
Tru9I TTAA 4 cut(s) 147, 251, 597, 736
TscAI CASTG 2 cut(s) 112, 504
TseI GCWGC 1 cut(s) 581
TspDTI ATGAA 1 cut(s) 309
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 2 cut(s) 112, 504
Vha464I CTTAAG 1 cut(s) 250
VpaK11BI GGWCC 3 cut(s) 280, 481, 606
XmiI GTMKAC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.