Rh4DG405000

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005509
Physical Location & Seq
Reverse (-)
61961728 .. 61963508
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG405000.1

Sequence Viewer

Length: 738 bp
ATGGCTACCAGACTTCAATTCGAGAACTCGTGCGAGGTTGGGGTGTTCTCGAAGCTGACGAATGCTTACTGTTTGGTCTCGATCGGAGGCTCCGAGAGCTTTTACAGCACATTTGAGGCGGAGCTGGCTGACGTCATCCCTGTCGTCAAGACCTCCATTGGAGGCACTCGCATCGTTGGTCGTCTCTGTGCAGGGAACAAAAATGGGCTGCTTTTGCCTCACACCACCACTGATCAAGAGCTGCAGCACCTGAGGAACAGCTTGCCTGATCAAGTTGTTGTTCAGCGAGTCGACGAGAGGCTATCTGCACTTGGCAACTGTATTGCGTGCAATGACCATGTTGCTCTTACGCATACTGATCTCGACAGGGAAACTGAGGAGATGATTGCAGATGTTCTTGGAGTGGAAGTCTTTAGGCAGACAATTGCTGGTAATATTCTTGTGGGCAGTTACTGTGCCATCTCCAACAAAGGTGGCTTGGTTCACCCGCATACATCCGTGGAAGACTTGGATGAACTTTCTACACTTCTTCAGGTTCCTCTTGTAGCTGGTACAGTCAACCGTGGTAGTGAAGTGATAGCTGCTGGCATGACTGTGAATGACTGGACAGCGTTCTGTGGCTCAGACACCACAGCCACAGAAGTCTCGGTTATTGAAAGTGTTTTCAAGTTGAGAGAGGCCCAACCTAGTGCCATTGTGGATGAAATGAGGAAATCATTGATCGACAGCTATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000460 GO:0000470 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010154 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033750 GO:0033993 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042221 GO:0042254 GO:0042273 GO:0042886 GO:0043021 GO:0043023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050896 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051716 GO:0061458 GO:0065003 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071426 GO:0071428 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097305 GO:0097306 GO:1901360 GO:1901700 GO:1901701 GO:1902626 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
eIF-6

Protein Analysis

245

Amino Acids

26.39

Weight (kDa)

4.69

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-6 PF01912 4 - 203 2.2e-81 eIF-6 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 291
AciI CCGC 2 cut(s) 119, 488
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 1 cut(s) 553
AgsI TTSAA 3 cut(s) 17, 656, 667
AluBI AGCT 8 cut(s) 55, 99, 124, 241, 261, 548, 581, 729
AluI AGCT 8 cut(s) 55, 99, 124, 241, 261, 548, 581, 729
Alw26I GTCTC 3 cut(s) 82, 188, 649
AlwNI CAGNNNCTG 2 cut(s) 250, 453
AoxI GGCC 1 cut(s) 678
ApeKI GCWGC 4 cut(s) 208, 241, 244, 581
AspS9I GGNCC 1 cut(s) 679
AsuHPI GGTGA 1 cut(s) 476
AxyI CCTNAGG 1 cut(s) 251
BauI CACGAG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 4 cut(s) 195, 228, 256, 568
BccI CCATC 1 cut(s) 467
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BclI TGATCA 2 cut(s) 232, 268
BcoDI GTCTC 3 cut(s) 82, 188, 649
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 1 cut(s) 242
BisI GCNGC 4 cut(s) 209, 242, 245, 582
BlsI GCNGC 4 cut(s) 210, 243, 246, 583
BmgT120I GGNCC 1 cut(s) 679
BmiI GGNNCC 2 cut(s) 91, 537
BmsI GCATC 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 498, 562
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bse1I ACTGG 1 cut(s) 608
Bse21I CCTNAGG 1 cut(s) 251
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 498, 562
BseGI GGATG 4 cut(s) 135, 494, 517, 706
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 3 cut(s) 242, 366, 636
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 4 cut(s) 195, 228, 256, 568
BsgI GTGCAG 2 cut(s) 210, 291
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 680
BsiEI CGRYCG 1 cut(s) 84
BsmAI GTCTC 3 cut(s) 82, 188, 649
BsmBI CGTCTC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 1 cut(s) 680
Bso31I GGTCTC 1 cut(s) 82
Bsp143I GATC 5 cut(s) 81, 232, 268, 358, 720
BspACI CCGC 2 cut(s) 119, 488
BspANI GGCC 1 cut(s) 680
BspCNI CTCAG 3 cut(s) 243, 367, 635
BspLI GGNNCC 2 cut(s) 91, 537
BspMAI CTGCAG 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 2 cut(s) 498, 562
BssMI GATC 5 cut(s) 81, 232, 268, 358, 720
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 28
Bst4CI ACNGT 6 cut(s) 71, 320, 455, 556, 563, 595
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 4 cut(s) 126, 263, 328, 586
BstDEI CTNAG 3 cut(s) 251, 375, 622
BstDSI CCRYGG 2 cut(s) 498, 562
BstF5I GGATG 4 cut(s) 135, 494, 517, 706
BstKTI GATC 5 cut(s) 84, 235, 271, 361, 723
BstMAI GTCTC 3 cut(s) 82, 188, 649
BstMBI GATC 5 cut(s) 81, 232, 268, 358, 720
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 4 cut(s) 96, 105, 125, 214
BstSFI CTRYAG 1 cut(s) 242
BstV1I GCAGC 4 cut(s) 195, 228, 256, 568
BstV2I GAAGAC 1 cut(s) 510
Bsu36I CCTNAGG 1 cut(s) 251
BsuRI GGCC 1 cut(s) 680
BtgI CCRYGG 2 cut(s) 498, 562
BtsCI GGATG 4 cut(s) 135, 494, 517, 706
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 4 cut(s) 126, 263, 328, 586
CaiI CAGNNNCTG 2 cut(s) 250, 453
Cfr13I GGNCC 1 cut(s) 679
Csp6I GTAC 1 cut(s) 552
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 2 cut(s) 338, 589
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 3 cut(s) 251, 375, 622
DpnI GATC 5 cut(s) 83, 234, 270, 360, 722
DpnII GATC 5 cut(s) 81, 232, 268, 358, 720
EciI GGCGGA 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 515
Eco81I CCTNAGG 1 cut(s) 251
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 2 cut(s) 341, 592
FaiI YATR 5 cut(s) 339, 354, 492, 590, 732
FatI CATG 2 cut(s) 337, 588
FauI CCCGC 1 cut(s) 495
FbaI TGATCA 2 cut(s) 232, 268
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 4 cut(s) 209, 242, 245, 582
FokI GGATG 4 cut(s) 122, 481, 524, 713
Fsp4HI GCNGC 4 cut(s) 209, 242, 245, 582
FspBI CTAG 1 cut(s) 687
GluI GCNGC 4 cut(s) 209, 242, 245, 582
HaeIII GGCC 1 cut(s) 680
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 2 cut(s) 341, 592
HincII GTYRAC 2 cut(s) 292, 559
HindII GTYRAC 2 cut(s) 292, 559
HinfI GANTC 1 cut(s) 288
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 3 cut(s) 292, 484, 559
Hpy188I TCNGA 3 cut(s) 86, 94, 625
Hpy188III TCNNGA 6 cut(s) 22, 49, 79, 148, 236, 362
Hpy8I GTNNAC 3 cut(s) 292, 484, 559
Hpy99I CGWCG 1 cut(s) 296
HpyCH4III ACNGT 6 cut(s) 71, 320, 455, 556, 563, 595
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 5 cut(s) 191, 244, 308, 330, 389
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 105, 125, 214
HpyF3I CTNAG 3 cut(s) 251, 375, 622
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 2 cut(s) 341, 592
Ksp22I TGATCA 2 cut(s) 232, 268
Kzo9I GATC 5 cut(s) 81, 232, 268, 358, 720
LmnI GCTCC 2 cut(s) 95, 121
Lsp1109I GCAGC 4 cut(s) 195, 228, 256, 568
LweI GCATC 1 cut(s) 180
MaeI CTAG 1 cut(s) 687
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 449
MalI GATC 5 cut(s) 83, 234, 270, 360, 722
MboI GATC 5 cut(s) 81, 232, 268, 358, 720
MboII GAAGA 2 cut(s) 515, 521
MfeI CAATTG 1 cut(s) 423
MluCI AATT 2 cut(s) 17, 423
MlyI GAGTC 1 cut(s) 297
MmeI TCCRAC 1 cut(s) 489
MseI TTAA 1 cut(s) 736
MslI CAYNNNNRTG 1 cut(s) 593
MunI CAATTG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 67
MwoI GCNNNNNNNGC 4 cut(s) 96, 105, 125, 214
NdeII GATC 5 cut(s) 81, 232, 268, 358, 720
NlaIII CATG 2 cut(s) 341, 592
NlaIV GGNNCC 2 cut(s) 91, 537
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 90
PctI GAATGC 1 cut(s) 67
PkrI GCNGC 4 cut(s) 210, 243, 246, 583
Ple19I CGATCG 1 cut(s) 84
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PspN4I GGNNCC 2 cut(s) 91, 537
PspPI GGNCC 1 cut(s) 679
PstI CTGCAG 1 cut(s) 246
PstNI CAGNNNCTG 2 cut(s) 250, 453
PvuI CGATCG 1 cut(s) 84
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 1 cut(s) 593
SalI GTCGAC 1 cut(s) 290
SaqAI TTAA 1 cut(s) 736
SatI GCNGC 4 cut(s) 209, 242, 245, 582
Sau3AI GATC 5 cut(s) 81, 232, 268, 358, 720
Sau96I GGNCC 1 cut(s) 679
SchI GAGTC 1 cut(s) 297
SfaNI GCATC 1 cut(s) 180
SfcI CTRYAG 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 593
Sse9I AATT 2 cut(s) 17, 423
SsiI CCGC 2 cut(s) 119, 488
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 687
TaaI ACNGT 6 cut(s) 71, 320, 455, 556, 563, 595
TaiI ACGT 1 cut(s) 135
TaqI TCGA 6 cut(s) 21, 50, 80, 291, 363, 723
TasI AATT 2 cut(s) 17, 423
Tru1I TTAA 1 cut(s) 736
Tru9I TTAA 1 cut(s) 736
TscAI CASTG 1 cut(s) 235
TseI GCWGC 4 cut(s) 208, 241, 244, 581
TspDTI ATGAA 2 cut(s) 528, 717
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 1 cut(s) 687
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.