RLG00000013362

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005509
Physical Location & Seq
Reverse (-)
30343248 .. 30344823
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013362

Sequence Viewer

Length: 738 bp
ATGGCGACCAGGCTGCAATTCGAGAACTCATGTGAGGTTGGGGTGTTCTCAAAGCTGAGTAATGCATACTGTTTGGTTGCCATTGGAGGCTCTGAGAGCTTCTACAGTACATTTGAGACTGAGTTGGCAGATGTCATCCCTGTGGTTAAGACCTCTATTGGCGGCACTCGCATCATTGGTCGCCTCTGTGTCGGGAACAAAAATGGGCTTCTTCTGCCTCACACCACCACAGACCAAGAACTACAACACTTGAGGAACAGCTTGCCTGACCAAGTCGTTGTTCAGCGCATTGAAGAGAGACTATCTGCTCTTGGTAACTGTATCGCGTGCAATGATCATGTGGCTCTTACACACCCTGATCTTGAAAGGGAAACTGAGGAGATGGTCGCAGATGTACTCGGGGTGGAGGTTTTTAGGAACACAATTGCAACTAATGTTCTTGTGGGCAGCTTTTGTGCCTTGTCCAACAGAGGTGGCTTGGTTCATCCACACACATCTGTTGAAGACTTGGATGAACTTTCAACACTTCTTCAGGTCCCTCTGGTTGCTGGGACAGTCAACCGTGGTAGTGAAGTGATAGCAGCTGGCATGACAGTGAATGATTGGACAGCATTCTGTGGGTCTGACACAACTGCAACAGAAGTCTCAGTTATTGAAAGTGTGTTCAAGTTGAGGGAAGCTCAACCTAGTGCCATTGTGGATGAAATGAGGAAGTCATTGATCGACAGCTATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000460 GO:0000470 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010154 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033750 GO:0033993 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042221 GO:0042254 GO:0042273 GO:0042886 GO:0043021 GO:0043023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050896 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051716 GO:0061458 GO:0065003 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071426 GO:0071428 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097305 GO:0097306 GO:1901360 GO:1901700 GO:1901701 GO:1902626 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
eIF-6

Protein Analysis

246

Amino Acids

26.48

Weight (kDa)

4.71

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-6 PF01912 4 - 203 3.3e-80 eIF-6 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 731
AccII CGCG 1 cut(s) 326
AciI CCGC 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 2 cut(s) 109, 396
AgsI TTSAA 6 cut(s) 293, 365, 503, 522, 656, 667
AjnI CCWGG 1 cut(s) 8
AjuI GAANNNNNNNTTGG 2 cut(s) 264, 296
AluBI AGCT 7 cut(s) 55, 99, 261, 450, 584, 680, 729
AluI AGCT 7 cut(s) 55, 99, 261, 450, 584, 680, 729
Alw26I GTCTC 3 cut(s) 110, 292, 649
Ama87I CYCGRG 1 cut(s) 398
ApeKI GCWGC 3 cut(s) 13, 447, 581
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 535
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 2 cut(s) 459, 593
BccI CCATC 1 cut(s) 376
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 10
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 110, 292, 649
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 1 cut(s) 103
BisI GCNGC 4 cut(s) 14, 163, 448, 582
BlsI GCNGC 4 cut(s) 15, 164, 449, 583
Bme1390I CCNGG 1 cut(s) 10
Bme18I GGWCC 1 cut(s) 535
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 510
BplI GAGNNNNNCTC 2 cut(s) 664, 696
BpuEI CTTGAG 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 562
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 562
BseGI GGATG 4 cut(s) 135, 484, 517, 706
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 5 cut(s) 47, 84, 111, 366, 660
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 2 cut(s) 459, 593
BseYI CCCAGC 1 cut(s) 548
Bsh1236I CGCG 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 398
BslFI GGGAC 2 cut(s) 521, 565
BsmAI GTCTC 3 cut(s) 110, 292, 649
BsmFI GGGAC 2 cut(s) 521, 565
BsmI GAATGC 1 cut(s) 611
BsoBI CYCGRG 1 cut(s) 398
Bsp143I GATC 3 cut(s) 334, 358, 720
BspACI CCGC 1 cut(s) 162
BspCNI CTCAG 5 cut(s) 48, 85, 112, 367, 659
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 562
BssMI GATC 3 cut(s) 334, 358, 720
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 6 cut(s) 71, 107, 320, 556, 563, 595
Bst6I CTCTTC 1 cut(s) 288
BstC8I GCNNGC 3 cut(s) 263, 328, 586
BstDEI CTNAG 5 cut(s) 56, 93, 120, 375, 646
BstDSI CCRYGG 1 cut(s) 562
BstF5I GGATG 4 cut(s) 135, 484, 517, 706
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 3 cut(s) 337, 361, 723
BstMAI GTCTC 3 cut(s) 110, 292, 649
BstMBI GATC 3 cut(s) 334, 358, 720
BstMWI GCNNNNNNNGC 3 cut(s) 96, 168, 214
BstNI CCWGG 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 8
BstSFI CTRYAG 1 cut(s) 103
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 2 cut(s) 459, 593
BstV2I GAAGAC 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 562
BtsCI GGATG 4 cut(s) 135, 484, 517, 706
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 3 cut(s) 263, 328, 586
CfoI GCGC 1 cut(s) 288
Cfr13I GGNCC 1 cut(s) 535
Csp6I GTAC 2 cut(s) 108, 395
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 3 cut(s) 30, 338, 589
CviQI GTAC 2 cut(s) 108, 395
DdeI CTNAG 5 cut(s) 56, 93, 120, 375, 646
DpnI GATC 3 cut(s) 336, 360, 722
DpnII GATC 3 cut(s) 334, 358, 720
DrdI GACNNNNNNGTC 1 cut(s) 731
DseDI GACNNNNNNGTC 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 398
EcoO109I RGGNCCY 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 8
EcoT22I ATGCAT 1 cut(s) 67
FaeI CATG 3 cut(s) 33, 341, 592
FaiI YATR 5 cut(s) 31, 67, 339, 590, 732
FaqI GGGAC 2 cut(s) 521, 565
FatI CATG 3 cut(s) 29, 337, 588
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 4 cut(s) 14, 163, 448, 582
FokI GGATG 4 cut(s) 122, 471, 524, 713
Fsp4HI GCNGC 4 cut(s) 14, 163, 448, 582
FspBI CTAG 1 cut(s) 687
GlaI GCGC 1 cut(s) 287
GluI GCNGC 4 cut(s) 14, 163, 448, 582
GsaI CCCAGC 1 cut(s) 552
HhaI GCGC 1 cut(s) 288
Hin1II CATG 3 cut(s) 33, 341, 592
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 3 cut(s) 94, 625, 737
Hpy188III TCNNGA 3 cut(s) 22, 193, 362
Hpy8I GTNNAC 1 cut(s) 559
HpyCH4III ACNGT 6 cut(s) 71, 107, 320, 556, 563, 595
HpyCH4V TGCA 5 cut(s) 16, 65, 330, 428, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 168, 214
HpyF3I CTNAG 5 cut(s) 56, 93, 120, 375, 646
Hsp92II CATG 3 cut(s) 33, 341, 592
HspAI GCGC 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 334, 358, 720
LpnPI CCDG 8 cut(s) 22, 153, 279, 369, 518, 527, 534, 570
Lsp1109I GCAGC 2 cut(s) 459, 593
LweI GCATC 1 cut(s) 180
MaeI CTAG 1 cut(s) 687
MaeIII GTNAC 1 cut(s) 314
MalI GATC 3 cut(s) 336, 360, 722
MboI GATC 3 cut(s) 334, 358, 720
MboII GAAGA 4 cut(s) 203, 305, 515, 521
MfeI CAATTG 1 cut(s) 423
MluCI AATT 2 cut(s) 17, 423
MmeI TCCRAC 1 cut(s) 489
Mph1103I ATGCAT 1 cut(s) 67
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 2 cut(s) 140, 593
MspA1I CMGCKG 1 cut(s) 584
MspR9I CCNGG 1 cut(s) 10
MunI CAATTG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 1 cut(s) 10
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 3 cut(s) 96, 168, 214
NdeII GATC 3 cut(s) 334, 358, 720
NlaIII CATG 3 cut(s) 33, 341, 592
NlaIV GGNNCC 1 cut(s) 537
NsiI ATGCAT 1 cut(s) 67
PctI GAATGC 1 cut(s) 611
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 15, 164, 449, 583
PpuMI RGGWCCY 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 1 cut(s) 8
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 537
PspPI GGNCC 1 cut(s) 535
PspPPI RGGWCCY 1 cut(s) 535
PsyI GACNNNGTC 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 2 cut(s) 109, 396
RsaNI GTAC 2 cut(s) 108, 395
RseI CAYNNNNRTG 2 cut(s) 140, 593
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 4 cut(s) 14, 163, 448, 582
Sau3AI GATC 3 cut(s) 334, 358, 720
Sau96I GGNCC 1 cut(s) 535
ScrFI CCNGG 1 cut(s) 10
SfaNI GCATC 1 cut(s) 180
SfcI CTRYAG 1 cut(s) 103
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 2 cut(s) 140, 593
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 2 cut(s) 17, 423
SsiI CCGC 1 cut(s) 162
SspMI CTAG 1 cut(s) 687
StyD4I CCNGG 1 cut(s) 8
TaaI ACNGT 6 cut(s) 71, 107, 320, 556, 563, 595
TaqI TCGA 2 cut(s) 21, 723
TasI AATT 2 cut(s) 17, 423
TatI WGTACW 2 cut(s) 107, 394
TauI GCSGC 1 cut(s) 165
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 600
TseI GCWGC 3 cut(s) 13, 447, 581
TspDTI ATGAA 3 cut(s) 473, 528, 717
TspRI CASTG 1 cut(s) 600
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 535
XspI CTAG 1 cut(s) 687
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.