MD05G1017600.v1.1

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005509
Physical Location & Seq
Reverse (-)
3066170 .. 3068581
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1017600.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGCAACCAGATTGCAATTCGAGAACTCGTGCGAGGTTGGGGTGTTTTCGAAGCTGACGAATGCGTACTGTTTGGTCTCCATCGGAGGCTCTGAGAACTTCTACAGCACCTTTGAGGCGGAGTTGGCTGATGTCATCCCCGTCGTGAAAACCTCCATCGGCGGCACTCGCATCATAGGCCGCCTCTGTGCTGGAAACAAAAACGGGCTTCTTTTGCCTCACACTACCACCGACCAAGAACTCCAGCACTTGAGGAACAGCTTACCTGATAATGTTGTTGTTCAGCGTATCGAAGAGAGGCTATCGGCTCTTGGTAACTGTATCGCGTGCAATGATCATGTTGCTCTTACACACACTGATCTTGACAGGGAAACTGAGGAGATGATTGCAGATGTTCTTGGAGTGGAAGTTTTTAGGCAGACTATTGCTGGTAATATTCTTGTGGGCAGCTACTGTTCCTTCACCAACAGAGGTGGCTTGGTTCATCCCCATACATCTGTTGAAGACTTGGATGAACTGTCGACGCTCCTTCAGGTCCCTCTTGTTGCTGGAACAGTAAACCGTGGTAGTGAAGTGATAGCTGCAGGCATGACAGTGAACGACTGGACAGCATTCTGCGGGTTAGACACCACTGCCACAGAACTCTCGGTTATTGAAAGCGTTTTCAAGTTGAGGGAAGCTCAACCTAGTGCTATTGTGGATGAAATGAGGAAATCTTTGATTGACAGCTATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000460 GO:0000470 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010154 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033750 GO:0033993 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042221 GO:0042254 GO:0042273 GO:0042886 GO:0043021 GO:0043023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050896 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051716 GO:0061458 GO:0065003 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071426 GO:0071428 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097305 GO:0097306 GO:1901360 GO:1901700 GO:1901701 GO:1902626 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
eIF-6

Protein Analysis

246

Amino Acids

26.57

Weight (kDa)

4.7

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-6 PF01912 4 - 203 8.4e-82 eIF-6 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 731
AccI GTMKAC 1 cut(s) 521
AccII CGCG 1 cut(s) 326
AciI CCGC 4 cut(s) 119, 162, 181, 618
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 1 cut(s) 68
AgsI TTSAA 3 cut(s) 503, 656, 667
AluBI AGCT 6 cut(s) 55, 261, 450, 581, 680, 729
AluI AGCT 6 cut(s) 55, 261, 450, 581, 680, 729
Alw26I GTCTC 1 cut(s) 82
AlwNI CAGNNNCTG 1 cut(s) 453
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 2 cut(s) 447, 581
AspS9I GGNCC 1 cut(s) 535
AsuHPI GGTGA 1 cut(s) 454
AsuII TTCGAA 1 cut(s) 50
AvaII GGWCC 1 cut(s) 535
BauI CACGAG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 2 cut(s) 459, 568
BccI CCATC 2 cut(s) 89, 164
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 2 cut(s) 103, 582
BisI GCNGC 4 cut(s) 163, 181, 448, 582
BlsI GCNGC 4 cut(s) 164, 182, 449, 583
Bme18I GGWCC 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 1 cut(s) 537
BmsI GCATC 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 510
BplI GAGNNNNNCTC 2 cut(s) 664, 696
BpmI CTGGAG 1 cut(s) 227
Bpu14I TTCGAA 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 271
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 562
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bse1I ACTGG 1 cut(s) 608
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 562
BseGI GGATG 4 cut(s) 135, 484, 517, 706
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 84, 366
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 2 cut(s) 459, 568
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 1 cut(s) 180
BslFI GGGAC 1 cut(s) 521
BsmAI GTCTC 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 2 cut(s) 67, 611
BsnI GGCC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 82
Bsp119I TTCGAA 1 cut(s) 50
Bsp143I GATC 2 cut(s) 334, 358
BspACI CCGC 4 cut(s) 119, 162, 181, 618
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 2 cut(s) 85, 367
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 537
BspMAI CTGCAG 1 cut(s) 586
BspT104I TTCGAA 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 562
BssMI GATC 2 cut(s) 334, 358
BssSI CACGAG 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 28
Bst4CI ACNGT 7 cut(s) 71, 320, 455, 519, 556, 563, 595
Bst6I CTCTTC 1 cut(s) 288
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 2 cut(s) 328, 586
BstDEI CTNAG 2 cut(s) 93, 375
BstDSI CCRYGG 1 cut(s) 562
BstF5I GGATG 4 cut(s) 135, 484, 517, 706
BstFNI CGCG 1 cut(s) 326
BstKTI GATC 2 cut(s) 337, 361
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 2 cut(s) 334, 358
BstMWI GCNNNNNNNGC 4 cut(s) 125, 168, 177, 214
BstSFI CTRYAG 2 cut(s) 103, 582
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 2 cut(s) 459, 568
BstV2I GAAGAC 1 cut(s) 510
BsuRI GGCC 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 562
BtsCI GGATG 4 cut(s) 135, 484, 517, 706
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 3 cut(s) 354, 600, 630
Cac8I GCNNGC 2 cut(s) 328, 586
CaiI CAGNNNCTG 1 cut(s) 453
Cfr13I GGNCC 1 cut(s) 535
CseI GACGC 1 cut(s) 532
Csp6I GTAC 1 cut(s) 67
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 2 cut(s) 338, 589
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 2 cut(s) 93, 375
DpnI GATC 2 cut(s) 336, 360
DpnII GATC 2 cut(s) 334, 358
DrdI GACNNNNNNGTC 1 cut(s) 731
DseDI GACNNNNNNGTC 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
EciI GGCGGA 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 515
EcoO109I RGGNCCY 1 cut(s) 535
FaeI CATG 2 cut(s) 341, 592
FaiI YATR 5 cut(s) 176, 339, 492, 590, 732
FaqI GGGAC 1 cut(s) 521
FatI CATG 2 cut(s) 337, 588
FauI CCCGC 1 cut(s) 611
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 1 cut(s) 521
Fnu4HI GCNGC 4 cut(s) 163, 181, 448, 582
FokI GGATG 4 cut(s) 122, 471, 524, 713
Fsp4HI GCNGC 4 cut(s) 163, 181, 448, 582
FspBI CTAG 1 cut(s) 687
GluI GCNGC 4 cut(s) 163, 181, 448, 582
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 1 cut(s) 180
HgaI GACGC 1 cut(s) 532
Hin1II CATG 2 cut(s) 341, 592
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 3 cut(s) 522, 559, 598
Hpy188I TCNGA 2 cut(s) 86, 94
Hpy188III TCNNGA 3 cut(s) 22, 145, 362
Hpy8I GTNNAC 3 cut(s) 522, 559, 598
Hpy99I CGWCG 2 cut(s) 146, 526
HpyAV CCTTC 2 cut(s) 469, 539
HpyCH4III ACNGT 7 cut(s) 71, 320, 455, 519, 556, 563, 595
HpyCH4V TGCA 4 cut(s) 16, 330, 389, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 168, 177, 214
HpyF3I CTNAG 2 cut(s) 93, 375
Hsp92II CATG 2 cut(s) 341, 592
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 334, 358
LmnI GCTCC 1 cut(s) 531
Lsp1109I GCAGC 2 cut(s) 459, 568
LweI GCATC 1 cut(s) 180
MaeI CTAG 1 cut(s) 687
MaeIII GTNAC 1 cut(s) 314
MalI GATC 2 cut(s) 336, 360
MboI GATC 2 cut(s) 334, 358
MboII GAAGA 2 cut(s) 305, 515
MluCI AATT 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 593
Mva1269I GAATGC 2 cut(s) 67, 611
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 4 cut(s) 125, 168, 177, 214
NdeII GATC 2 cut(s) 334, 358
NlaIII CATG 2 cut(s) 341, 592
NlaIV GGNNCC 1 cut(s) 537
NspV TTCGAA 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PctI GAATGC 2 cut(s) 67, 611
PkrI GCNGC 4 cut(s) 164, 182, 449, 583
PpuMI RGGWCCY 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 535
PspN4I GGNNCC 1 cut(s) 537
PspPI GGNCC 1 cut(s) 535
PspPPI RGGWCCY 1 cut(s) 535
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 1 cut(s) 453
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
RseI CAYNNNNRTG 1 cut(s) 593
SalI GTCGAC 1 cut(s) 520
SatI GCNGC 4 cut(s) 163, 181, 448, 582
Sau3AI GATC 2 cut(s) 334, 358
Sau96I GGNCC 1 cut(s) 535
SfaNI GCATC 1 cut(s) 180
SfcI CTRYAG 2 cut(s) 103, 582
SfuI TTCGAA 1 cut(s) 50
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 1 cut(s) 17
SsiI CCGC 4 cut(s) 119, 162, 181, 618
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 687
TaaI ACNGT 7 cut(s) 71, 320, 455, 519, 556, 563, 595
TaqI TCGA 4 cut(s) 21, 50, 291, 521
TasI AATT 1 cut(s) 17
TauI GCSGC 2 cut(s) 165, 183
TscAI CASTG 3 cut(s) 361, 600, 637
TseI GCWGC 2 cut(s) 447, 581
TspDTI ATGAA 3 cut(s) 473, 528, 717
TspRI CASTG 3 cut(s) 361, 600, 637
VpaK11BI GGWCC 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 521
XspI CTAG 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.