Rorug06G0104400

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005509
Physical Location & Seq
Forward (+)
13789243 .. 13792919
3677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0104400.1

Sequence Viewer

Length: 3141 bp
ATGGATCTAAGGAAACCTCAATTCGGAGCCTCTTCCTCCTCCTCTTCTTCTTCCACCCATTCGTCCACACACGATGTCTTTCTGAGCTTTAGAGGTGCTGATACGCGCTACAATTTCACAGGCCATTTGCACAGCAATTTGGTTAACAAGGGATTTGAGACCTTCATAGATGATGGGCTTCCGAAAGGAGAAGATATATCACAAGAGCTTCTCGAAGTAATTGAGAGATCAAAGATTTCCATAGTTGTATTTTCTGCGAACTATGCATCTTCAAAGTGGTGCTTGGATGAACTTGTCAAGATCATTCAATGTAAAGAATCCAAGCACCAAATTGTTTACCCAATTTTCTACAAGGTAGATCCGTCGGAGATACGATACCAGAAGGGCCGGGTTGGTGAGGCAATTGCTCGCCTTGGCAAACACAAGGAGAACTTAGAGAAGGTGGAGAATTGGAAGGCAGCTCTTAGAAAAGCAGCAACTTTCTCTGGGTGGCACATCTCAGAAGGAGGGCATGAAGCGAATGCCATTGATGAAATTGTTAAACAGATATCAACTGAAATAATGAAATGCACCCCTTTAGATGTGGCAACATATCCTGTTGGAATAGAATCTCGTGTAAAAGACATACTTGAGTACTTAAATGTAGGGCAAGACAATCCTCACATGGTAGGGATATGGGGAATTGGCGGAATAGGAAAGTCAACACTTGCAAAAGCTGTTTACAATTCAAGTAGCCATGAGTTTGAACACAGATGTTTCTTGAAAATTGATTCATTTTCACAAGGAGGTCTTGTCCAGCTACAAAACGATTTTCTTTCTAAGATTATAGAGAGGAATGCACCTAAGGTGACCAATGTTGATGAAGGAATCACTCTGCTGAAGCAAAAACTAAGCCAGAAAAGGGTTCTCTTAGTTATTGATAATGTGAACCACTTGGACCAGTTAAGAGCACTTGCTGGAGGGTGTGATTGGTTTGGTCCAAGTAGTAGAATTATCATAACAACAAGAGATACAAATTTGCTAAATGCTCATGGAGTCGATTTCGATTCAATATATGAGGTCAAGGAATTAAATCATCAAGATGCTTCAAAGCTCTTCAATTTAAATGCCTTCAAAAGAGATATATGTCCGGATGACTTCTTTGAACTTGCAACTGAGGTAATACATTATGCTAAAGGGATTCCATTAGTTTTGGAAGTTTTGGGGTCAGATTTATGTAGTAAAAAGAATCAGGATGAGTGGAAAGCTGCAGTAGAGTATTACAACAAATATCCTAACCAAGAGATTCAACAAGCTCTCAAAAGAAGTTACGAGGCATTGGAGGATCAGATGAAAGAAGTTTTTCTTCATATTGCATGTTTCTTTAAAGGTCACAAACAAAGCTATGTGATAAATGCCCTACAAAGCTGTGACCTCAATCCTATGTATAGTCTCAAAGTCCTCGTAGAAAAGGCCTTAATTAAAATTGTGAAATACAGTGACAATGACAATGAGATTTGGATGCATGACCTTCTAGAAGACATGGGAAAAGAAATTGTTCGACAAGAGTCTCCTGATGAGATAGGACAACGCAGTAGACTGTGGCAATATGAGCATGTTCGCTACATCCTTGAGGAAAACACTGGAACGGAGAAAATTAAAGGCATCAGCTGTTCACAACCGATAACATTGAACGCCGGAAGCTTCACAAACTTGAAAAATCTTCAAATTTTTATAGTCGTTAATTTGACATTTCATGGAGAGAGCGTGGATGATCTCCCCAACCAGTTGAGGGTGCTTGACATAGGTGGGCCCTGTATGACTCCACTAGTGAACGAGGGATTGAAGAATCTGCGATATTTGAAATCTATCAAATTGCAGCGCTGTGATGGTCTAACCAGAATTCCCGACTTGTCTGGATTAACAAGCTTAGAGTATTTGGAGCTGTCTCATTGTGAAGACTTGGTTGAAGTTCATCCTTCGGTTGGCCGTCTCGATAAGCTCGTCATTTTGAAACTCACCCATTGTGAAAAGCTGCAGATGTTTCCGAAGCTGATCAACCTGAAATCTTTGGAAACTCTGGATCTCTCTTGGTGCCCGCTCAAGTTCTTCCCTGAAATCGAGGGAGACATGAAGTCTTTGAGTCACCTGGATCTAAGTCGCACTGCCATCAAAGAGTTACCTTGCTCAGTTGGAAATCTCACTGGCCTTAAATCCTTGTCTCTACGTGACTGTGATAATCTTACAAATGTACCATCAAGCATTTTCTATGGATTGCAACGTCTGGAAAGGCTTGATCTAGGGTACTGCGATAACCTTGTTACATTTCCAACAAAGTCAGAATCACTTCCTCCACCGGTCTTTTCAACTAACCTGACCGGATTACAAGTTCATCTGAACGGTTGCAAGCGGCTTGAAGAAATTTCAGAATTTCCACGAGAAATAGATTTCTTATGTGTGTGGGGTTGTGAGTCGTTGGAAAGAATTTCAAAGTTGTCAAAAATTTTGGAGGGTAAAGATACAAAAATGTTCCGTCGGTTGAACTTGACTTCCTGCTGGAGACTCTGCGACAATCTGGCAGAGGTTGAAGTTGACAAGCGACTATTTCGTATGGTGTTTGCGGGAACCAAAGAGTTGGACTTGACTTCCTGCTGGAGAGACTGCGACAATCTGGCAGATGTTGAAGTTGACAAGCGACTATTGCTTATGCCGTTTACGTTAACCAAAGAACTGACGGTTCTGTTGCCTCTTTTTTTCTCTTGTGCGAAATCTGAAGAATTCGAAGTAAAATTTCCTGCACGTGATCCGCTTCCAAACTATGTGAAAGGTGAAGTGATAAGTAAAGAGCATGAGTTTTGCATTGAAATTCCTCAAAACTCCAATTGGGACAACAAAGGGTTGGCTCTCTGTATTCAATCGTGTTCTTATATGCTCATTCATTTTGTTTACATCAACGGAATAAAGTTTGATGAGGTACTCATATTTGAGGACACAGTCTGGGTGCATTATATTCCATTCGTTACAGTAATAAGGAGGTTGAGTGAGTGTGAGATGCCGCCACCGGATATGTTTCGAGTTATGTTTCGTTGTGAAATCGAAGAGGATGTCAGGGGAACCTGGGGAGTCCACCTGATCGAAGATTTGGAAGGAGAAGGCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000460 GO:0000470 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010154 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033750 GO:0033993 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042221 GO:0042254 GO:0042273 GO:0042886 GO:0043021 GO:0043023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050896 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051716 GO:0061458 GO:0065003 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071426 GO:0071428 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097305 GO:0097306 GO:1901360 GO:1901700 GO:1901701 GO:1902626 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
eIF-6

Protein Analysis

1046

Amino Acids

119.24

Weight (kDa)

5.59

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 192 9e-52 TIR domain
TIR_2 PF13676 26 - 121 4.8e-14 TIR domain
NB-ARC PF00931 203 - 374 2.1e-18 NB-ARC domain
WHD_ROQ1 PF23282 443 - 517 1.1e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 589 - 690 9.9e-06 Leucine-rich repeat region
LRR_13 PF23286 681 - 766 5e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 705 - 765 3e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2073
AccBSI CCGCTC 1 cut(s) 2080
AccI GTMKAC 1 cut(s) 1576
AccII CGCG 1 cut(s) 106
AccIII TCCGGA 1 cut(s) 1129
AciI CCGC 6 cut(s) 687, 2078, 2389, 2600, 2786, 3035
AclWI GGATC 6 cut(s) 12, 353, 1332, 2070, 2139, 2777
AcoI YGGCCR 1 cut(s) 1966
AcuI CTGAAG 2 cut(s) 899, 2772
AcvI CACGTG 1 cut(s) 2780
AfaI GTAC 4 cut(s) 635, 2232, 2285, 2955
AfeI AGCGCT 1 cut(s) 1862
AfiI CCNNNNNNNGG 4 cut(s) 23, 901, 1771, 1964
AgeI ACCGGT 1 cut(s) 2335
AhdI GACNNNNNGTC 1 cut(s) 2113
AhlI ACTAGT 1 cut(s) 1807
AjnI CCWGG 2 cut(s) 2127, 3095
AloI GAACNNNNNNTCC 4 cut(s) 2352, 2384, 2513, 2545
Alw21I GWGCWC 1 cut(s) 952
Alw26I GTCTC 9 cut(s) 152, 1436, 1554, 1932, 1976, 2100, 2205, 2533, 2631
AlwI GGATC 6 cut(s) 12, 353, 1332, 2070, 2139, 2777
Aor13HI TCCGGA 1 cut(s) 1129
Aor51HI AGCGCT 1 cut(s) 1862
AoxI GGCC 7 cut(s) 121, 385, 1452, 1790, 1966, 2185, 3133
ApaI GGGCCC 1 cut(s) 1794
ApeKI GCWGC 5 cut(s) 458, 473, 1247, 1858, 2014
ArsI GACNNNNNNTTYG 2 cut(s) 3069, 3101
AsiGI ACCGGT 1 cut(s) 2335
Asp700I GAANNNNTTC 3 cut(s) 1341, 1536, 2325
AspLEI GCGC 2 cut(s) 108, 1863
AspS9I GGNCC 5 cut(s) 385, 937, 977, 1790, 1791
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 5 cut(s) 407, 859, 1990, 2117, 2819
AsuII TTCGAA 1 cut(s) 2760
AvaII GGWCC 2 cut(s) 937, 977
AxyI CCTNAGG 1 cut(s) 843
BaeGI GKGCMC 2 cut(s) 1794, 2078
BaeI ACNNNNGTAYC 2 cut(s) 2214, 2247
BanI GGYRCC 1 cut(s) 2073
BanII GRGCYC 1 cut(s) 1794
BauI CACGAG 2 cut(s) 612, 2415
BbrPI CACGTG 1 cut(s) 2780
BbsI GAAGAC 2 cut(s) 1524, 1944
Bbv12I GWGCWC 1 cut(s) 952
BbvI GCAGC 5 cut(s) 470, 485, 1234, 1870, 2001
BccI CCATC 4 cut(s) 167, 1862, 2156, 2242
BceAI ACGGC 2 cut(s) 1953, 2674
BciT130I CCWGG 2 cut(s) 2129, 3097
BclI TGATCA 1 cut(s) 2034
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 9 cut(s) 152, 1436, 1554, 1932, 1976, 2100, 2205, 2533, 2631
BcuI ACTAGT 1 cut(s) 1807
BfaI CTAG 3 cut(s) 1514, 1808, 2279
BfmI CTRYAG 2 cut(s) 1248, 2015
BfoI RGCGCY 1 cut(s) 1864
BisI GCNGC 7 cut(s) 459, 474, 1248, 1859, 2015, 2390, 3035
BlsI GCNGC 7 cut(s) 460, 475, 1249, 1860, 2016, 2391, 3036
BmcAI AGTACT 1 cut(s) 635
Bme1390I CCNGG 3 cut(s) 389, 2129, 3097
Bme18I GGWCC 2 cut(s) 937, 977
BmeRI GACNNNNNGTC 1 cut(s) 2113
BmgT120I GGNCC 5 cut(s) 385, 937, 977, 1790, 1791
BmiI GGNNCC 5 cut(s) 28, 1792, 2075, 2605, 3094
BmrFI CCNGG 3 cut(s) 389, 2129, 3097
BmsI GCATC 5 cut(s) 275, 1072, 1491, 1653, 3021
BoxI GACNNNNGTC 1 cut(s) 1546
BpiI GAAGAC 2 cut(s) 1524, 1944
BpmI CTGGAG 3 cut(s) 978, 2557, 2653
Bpu14I TTCGAA 1 cut(s) 2760
BpuEI CTTGAG 3 cut(s) 650, 1631, 2066
BpuMI CCSGG 1 cut(s) 389
BsaAI YACGTR 2 cut(s) 2207, 2780
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 412, 3096
BsaWI WCCGGW 4 cut(s) 1129, 2335, 2357, 3040
BsaXI ACNNNNNCTCC 6 cut(s) 777, 807, 1731, 1761, 3004, 3034
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 901, 1771, 1964
Bse118I RCCGGY 1 cut(s) 2335
Bse1I ACTGG 4 cut(s) 940, 1627, 1765, 2188
Bse21I CCTNAGG 1 cut(s) 843
BseAI TCCGGA 1 cut(s) 1129
BseBI CCWGG 2 cut(s) 2129, 3097
BseDI CCNNGG 2 cut(s) 412, 3096
BseGI GGATG 8 cut(s) 292, 1138, 1240, 1506, 1605, 1756, 1954, 3088
BseLI CCNNNNNNNGG 4 cut(s) 23, 901, 1771, 1964
BseMII CTCAG 4 cut(s) 74, 513, 1146, 2181
BseNI ACTGG 4 cut(s) 940, 1627, 1765, 2188
BseRI GAGGAG 2 cut(s) 28, 31
BseSI GKGCMC 2 cut(s) 1794, 2078
BseXI GCAGC 5 cut(s) 470, 485, 1234, 1870, 2001
BsgI GTGCAG 1 cut(s) 2760
Bsh1236I CGCG 1 cut(s) 106
BshFI GGCC 7 cut(s) 123, 387, 1454, 1792, 1968, 2187, 3135
BshNI GGYRCC 1 cut(s) 2073
BshTI ACCGGT 1 cut(s) 2335
BsiHKAI GWGCWC 1 cut(s) 952
BsiSI CCGG 6 cut(s) 388, 1130, 1677, 2336, 2358, 3041
BslFI GGGAC 1 cut(s) 2879
BslI CCNNNNNNNGG 4 cut(s) 23, 901, 1771, 1964
BsmAI GTCTC 9 cut(s) 152, 1436, 1554, 1932, 1976, 2100, 2205, 2533, 2631
BsmBI CGTCTC 1 cut(s) 1976
BsmFI GGGAC 1 cut(s) 2879
BsmI GAATGC 2 cut(s) 526, 841
BsnI GGCC 7 cut(s) 123, 387, 1454, 1792, 1968, 2187, 3135
Bso31I GGTCTC 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 2760
Bsp120I GGGCCC 1 cut(s) 1790
Bsp1286I GDGCHC 3 cut(s) 952, 1794, 2078
Bsp13I TCCGGA 1 cut(s) 1129
BspACI CCGC 6 cut(s) 687, 2078, 2389, 2600, 2786, 3035
BspANI GGCC 7 cut(s) 123, 387, 1454, 1792, 1968, 2187, 3135
BspCNI CTCAG 4 cut(s) 75, 512, 1147, 2180
BspEI TCCGGA 1 cut(s) 1129
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 5 cut(s) 28, 1792, 2075, 2605, 3094
BspMAI CTGCAG 2 cut(s) 1252, 2019
BspPI GGATC 6 cut(s) 12, 353, 1332, 2070, 2139, 2777
BspQI GCTCTTC 1 cut(s) 1100
BspT104I TTCGAA 1 cut(s) 2760
BspT107I GGYRCC 1 cut(s) 2073
BspTNI GGTCTC 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 2080
BsrFI RCCGGY 1 cut(s) 2335
BsrI ACTGG 4 cut(s) 940, 1627, 1765, 2188
BssAI RCCGGY 1 cut(s) 2335
BssECI CCNNGG 2 cut(s) 412, 3096
BssSI CACGAG 2 cut(s) 612, 2415
BssT1I CCWWGG 1 cut(s) 412
Bst2BI CACGAG 2 cut(s) 612, 2415
Bst2UI CCWGG 2 cut(s) 2129, 3097
Bst4CI ACNGT 7 cut(s) 1478, 1581, 2213, 2381, 2716, 2974, 3004
Bst6I CTCTTC 4 cut(s) 37, 49, 1100, 3072
BstBAI YACGTR 2 cut(s) 2207, 2780
BstBI TTCGAA 1 cut(s) 2760
BstC8I GCNNGC 3 cut(s) 409, 2078, 2387
BstEII GGTNACC 1 cut(s) 847
BstF5I GGATG 8 cut(s) 292, 1138, 1240, 1506, 1605, 1756, 1954, 3088
BstFNI CGCG 1 cut(s) 106
BstH2I RGCGCY 1 cut(s) 1864
BstHHI GCGC 2 cut(s) 108, 1863
BstMAI GTCTC 9 cut(s) 152, 1436, 1554, 1932, 1976, 2100, 2205, 2533, 2631
BstMWI GCNNNNNNNGC 3 cut(s) 263, 1591, 2680
BstNI CCWGG 2 cut(s) 2129, 3097
BstNSI RCATGY 2 cut(s) 1359, 1598
BstPAI GACNNNNGTC 1 cut(s) 1546
BstPI GGTNACC 1 cut(s) 847
BstSCI CCNGG 3 cut(s) 387, 2127, 3095
BstSFI CTRYAG 2 cut(s) 1248, 2015
BstSLI GKGCMC 2 cut(s) 1794, 2078
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 5 cut(s) 470, 485, 1234, 1870, 2001
BstV2I GAAGAC 2 cut(s) 1524, 1944
BstX2I RGATCY 4 cut(s) 4, 358, 2062, 2131
BstXI CCANNNNNNTGG 1 cut(s) 2614
BstYI RGATCY 4 cut(s) 4, 358, 2062, 2131
Bsu36I CCTNAGG 1 cut(s) 843
BsuRI GGCC 7 cut(s) 123, 387, 1454, 1792, 1968, 2187, 3135
BtsCI GGATG 8 cut(s) 292, 1138, 1240, 1506, 1605, 1756, 1954, 3088
BtsI GCAGTG 1 cut(s) 2142
BtsIMutI CAGTG 4 cut(s) 1483, 1620, 2142, 2181
Cac8I GCNNGC 3 cut(s) 409, 2078, 2387
CfoI GCGC 2 cut(s) 108, 1863
Cfr10I RCCGGY 1 cut(s) 2335
Cfr13I GGNCC 5 cut(s) 385, 937, 977, 1790, 1791
Csp6I GTAC 4 cut(s) 634, 2231, 2284, 2954
CspAI ACCGGT 1 cut(s) 2335
CviQI GTAC 4 cut(s) 634, 2231, 2284, 2954
DraI TTTAAA 2 cut(s) 1104, 1366
DriI GACNNNNNGTC 1 cut(s) 2113
EaeI YGGCCR 1 cut(s) 1966
Eam1104I CTCTTC 4 cut(s) 37, 49, 1100, 3072
Eam1105I GACNNNNNGTC 1 cut(s) 2113
EarI CTCTTC 4 cut(s) 37, 49, 1100, 3072
EciI GGCGGA 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 412
Eco147I AGGCCT 1 cut(s) 1454
Eco24I GRGCYC 1 cut(s) 1794
Eco31I GGTCTC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 937, 977
Eco47III AGCGCT 1 cut(s) 1862
Eco57I CTGAAG 2 cut(s) 899, 2772
Eco72I CACGTG 1 cut(s) 2780
Eco81I CCTNAGG 1 cut(s) 843
Eco91I GGTNACC 1 cut(s) 847
EcoO109I RGGNCCY 1 cut(s) 1791
EcoO65I GGTNACC 1 cut(s) 847
EcoRI GAATTC 2 cut(s) 1881, 2756
EcoRII CCWGG 2 cut(s) 2127, 3095
EcoRV GATATC 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 412
EcoT22I ATGCAT 2 cut(s) 268, 1506
EcoT38I GRGCYC 1 cut(s) 1794
ErhI CCWWGG 1 cut(s) 412
Esp3I CGTCTC 1 cut(s) 1976
FaqI GGGAC 1 cut(s) 2879
FauI CCCGC 2 cut(s) 2085, 2593
FbaI TGATCA 1 cut(s) 2034
FblI GTMKAC 1 cut(s) 1576
Fnu4HI GCNGC 7 cut(s) 459, 474, 1248, 1859, 2015, 2390, 3035
FokI GGATG 8 cut(s) 299, 1145, 1247, 1513, 1592, 1763, 1941, 3095
FriOI GRGCYC 1 cut(s) 1794
Fsp4HI GCNGC 7 cut(s) 459, 474, 1248, 1859, 2015, 2390, 3035
FspBI CTAG 3 cut(s) 1514, 1808, 2279
GlaI GCGC 2 cut(s) 107, 1862
GluI GCNGC 7 cut(s) 459, 474, 1248, 1859, 2015, 2390, 3035
GsuI CTGGAG 3 cut(s) 978, 2557, 2653
HaeII RGCGCY 1 cut(s) 1864
HaeIII GGCC 7 cut(s) 123, 387, 1454, 1792, 1968, 2187, 3135
HapII CCGG 6 cut(s) 388, 1130, 1677, 2336, 2358, 3041
HhaI GCGC 2 cut(s) 108, 1863
Hin6I GCGC 2 cut(s) 106, 1861
HinP1I GCGC 2 cut(s) 106, 1861
HincII GTYRAC 5 cut(s) 145, 702, 2572, 2668, 2700
HindII GTYRAC 5 cut(s) 145, 702, 2572, 2668, 2700
HindIII AAGCTT 2 cut(s) 1681, 1906
HpaI GTTAAC 2 cut(s) 145, 2700
HpaII CCGG 6 cut(s) 388, 1130, 1677, 2336, 2358, 3041
HphI GGTGA 5 cut(s) 407, 859, 1990, 2117, 2819
Hpy99I CGWCG 2 cut(s) 367, 2517
HpyCH4III ACNGT 7 cut(s) 1478, 1581, 2213, 2381, 2716, 2974, 3004
HpyCH4IV ACGT 4 cut(s) 2206, 2260, 2696, 2779
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 1591, 2680
HpySE526I ACGT 4 cut(s) 2206, 2260, 2696, 2779
HspAI GCGC 2 cut(s) 106, 1861
Kpn2I TCCGGA 1 cut(s) 1129
Ksp22I TGATCA 1 cut(s) 2034
KspAI GTTAAC 2 cut(s) 145, 2700
LguI GCTCTTC 1 cut(s) 1100
LmnI GCTCC 2 cut(s) 26, 1921
Lsp1109I GCAGC 5 cut(s) 470, 485, 1234, 1870, 2001
LweI GCATC 5 cut(s) 275, 1072, 1491, 1653, 3021
MaeI CTAG 3 cut(s) 1514, 1808, 2279
MaeII ACGT 4 cut(s) 2206, 2260, 2696, 2779
MbiI CCGCTC 1 cut(s) 2080
MfeI CAATTG 2 cut(s) 402, 2860
MflI RGATCY 4 cut(s) 4, 358, 2062, 2131
MhlI GDGCHC 3 cut(s) 952, 1794, 2078
MlyI GAGTC 7 cut(s) 1044, 1556, 1795, 2131, 2459, 2535, 3111
MmeI TCCRAC 6 cut(s) 345, 580, 2152, 2333, 2436, 2595
Mph1103I ATGCAT 2 cut(s) 268, 1506
MroI TCCGGA 1 cut(s) 1129
MroXI GAANNNNTTC 3 cut(s) 1341, 1536, 2325
MslI CAYNNNNRTG 1 cut(s) 1080
MspA1I CMGCKG 1 cut(s) 1650
MspI CCGG 6 cut(s) 388, 1130, 1677, 2336, 2358, 3041
MspR9I CCNGG 3 cut(s) 389, 2129, 3097
MunI CAATTG 2 cut(s) 402, 2860
Mva1269I GAATGC 2 cut(s) 526, 841
MvaI CCWGG 2 cut(s) 2129, 3097
MvnI CGCG 1 cut(s) 106
MwoI GCNNNNNNNGC 3 cut(s) 263, 1591, 2680
NciI CCSGG 1 cut(s) 389
NlaIV GGNNCC 5 cut(s) 28, 1792, 2075, 2605, 3094
NmuCI GTSAC 6 cut(s) 847, 1370, 1409, 1478, 2123, 2207
NsiI ATGCAT 2 cut(s) 268, 1506
NspI RCATGY 2 cut(s) 1359, 1598
NspV TTCGAA 1 cut(s) 2760
PacI TTAATTAA 1 cut(s) 1461
PceI AGGCCT 1 cut(s) 1454
PciSI GCTCTTC 1 cut(s) 1100
PcsI WCGNNNNNNNCGW 1 cut(s) 1980
PctI GAATGC 2 cut(s) 526, 841
PdmI GAANNNNTTC 3 cut(s) 1341, 1536, 2325
PflFI GACNNNGTC 1 cut(s) 2972
PinAI ACCGGT 1 cut(s) 2335
PkrI GCNGC 7 cut(s) 460, 475, 1249, 1860, 2016, 2391, 3036
PleI GAGTC 7 cut(s) 1043, 1555, 1795, 2130, 2458, 2535, 3110
PmaCI CACGTG 1 cut(s) 2780
PmlI CACGTG 1 cut(s) 2780
PpsI GAGTC 7 cut(s) 1043, 1555, 1795, 2130, 2458, 2535, 3110
Ppu21I YACGTR 2 cut(s) 2207, 2780
PshAI GACNNNNGTC 1 cut(s) 1546
Psp6I CCWGG 2 cut(s) 2127, 3095
PspCI CACGTG 1 cut(s) 2780
PspEI GGTNACC 1 cut(s) 847
PspGI CCWGG 2 cut(s) 2127, 3095
PspN4I GGNNCC 5 cut(s) 28, 1792, 2075, 2605, 3094
PspOMI GGGCCC 1 cut(s) 1790
PspPI GGNCC 5 cut(s) 385, 937, 977, 1790, 1791
PstI CTGCAG 2 cut(s) 1252, 2019
PsuI RGATCY 4 cut(s) 4, 358, 2062, 2131
PsyI GACNNNGTC 1 cut(s) 2972
PvuII CAGCTG 1 cut(s) 1650
RsaI GTAC 4 cut(s) 635, 2232, 2285, 2955
RsaNI GTAC 4 cut(s) 634, 2231, 2284, 2954
RseI CAYNNNNRTG 1 cut(s) 1080
SapI GCTCTTC 1 cut(s) 1100
SatI GCNGC 7 cut(s) 459, 474, 1248, 1859, 2015, 2390, 3035
Sau96I GGNCC 5 cut(s) 385, 937, 977, 1790, 1791
ScaI AGTACT 1 cut(s) 635
SchI GAGTC 7 cut(s) 1044, 1556, 1795, 2131, 2459, 2535, 3111
ScrFI CCNGG 3 cut(s) 389, 2129, 3097
SduI GDGCHC 3 cut(s) 952, 1794, 2078
SfaNI GCATC 5 cut(s) 275, 1072, 1491, 1653, 3021
SfcI CTRYAG 2 cut(s) 1248, 2015
SfuI TTCGAA 1 cut(s) 2760
SinI GGWCC 2 cut(s) 937, 977
SmiI ATTTAAAT 1 cut(s) 1104
SmiMI CAYNNNNRTG 1 cut(s) 1080
SmlI CTYRAG 3 cut(s) 629, 1610, 2081
SmoI CTYRAG 3 cut(s) 629, 1610, 2081
SpeI ACTAGT 1 cut(s) 1807
SseBI AGGCCT 1 cut(s) 1454
SsiI CCGC 6 cut(s) 687, 2078, 2389, 2600, 2786, 3035
SspMI CTAG 3 cut(s) 1514, 1808, 2279
StuI AGGCCT 1 cut(s) 1454
StyD4I CCNGG 3 cut(s) 387, 2127, 3095
StyI CCWWGG 1 cut(s) 412
SwaI ATTTAAAT 1 cut(s) 1104
TaaI ACNGT 7 cut(s) 1478, 1581, 2213, 2381, 2716, 2974, 3004
TaiI ACGT 4 cut(s) 2209, 2263, 2699, 2782
TatI WGTACW 1 cut(s) 633
TauI GCSGC 2 cut(s) 2392, 3037
TscAI CASTG 4 cut(s) 1483, 1627, 2149, 2188
TseFI GTSAC 6 cut(s) 847, 1370, 1409, 1478, 2123, 2207
TseI GCWGC 5 cut(s) 458, 473, 1247, 1858, 2014
Tsp45I GTSAC 6 cut(s) 847, 1370, 1409, 1478, 2123, 2207
TspGWI ACGGA 4 cut(s) 351, 1643, 2501, 2949
TspRI CASTG 4 cut(s) 1483, 1627, 2149, 2188
Tth111I GACNNNGTC 1 cut(s) 2972
VpaK11BI GGWCC 2 cut(s) 937, 977
XbaI TCTAGA 1 cut(s) 1513
XceI RCATGY 2 cut(s) 1359, 1598
XmiI GTMKAC 1 cut(s) 1576
XmnI GAANNNNTTC 3 cut(s) 1341, 1536, 2325
XspI CTAG 3 cut(s) 1514, 1808, 2279
ZrmI AGTACT 1 cut(s) 635
Zsp2I ATGCAT 2 cut(s) 268, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.