AT3G56330

N2,N2-dimethylguanosine tRNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009588
Physical Location & Seq
Forward (+)
20890235 .. 20892002
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G56330.1

Sequence Viewer

Length: 1302 bp
ATGTTGTTGACTCTCTCTCCTAAAACCCTATCTTCTTCTTCCTTCTTCACTGTGCACAAATCGCAAAACCCTAAATGTAAATCTCCAGATTCATGTCGATTCAAGAGCTTGAAATGTAGTTTTGATCGAATTTTAGTGAAATCCGAGGTTCAAATTGAGAGGAATCTCGAATTTGAAACGGGAGAGACATTTTTCCGCCATGAAAGCGCTAGAGGACGAGACCTCGGTGTTTTGTCAGCGACGCTATACAAGAGATCTAATGGAAGCTTACGGGTACTCGACGCAATGTGCGGATGTGGAATTAGGTCGCTTCGTTATCTTGTGGAAGCTGAAGCTGATTTCGTGATGGCGAACGACGCAAATGATGATAATCGGAGAGTGATTACGGATAATTTGAGTAAAGTAGAGAGAGGTACTGGAGATGAGAGGAGATGGGTTGTGACTCATATGTTGGCTAATAAAGCGATGATTGAGAGATATATGGTTGCGGATTTCTTCGATATGATTGATATTGATTCGTTTGGAAGTGATTCTTCGTTTTTGAGAGATGCGTTTAATGCATTGAGATTGGGTGGTTTGCTTTACTTGACGTCGACTGATGGTTACTCTTCTGGTGGTCACAGACCTTATAATTCTTTAGCAGCTTATGGAGCATTTATTCGACCAATGCCGTTTGGGAATGAGATTGGGCTGCGGATGCTTATAGGTGGAGCAGTGAGAGAAGCTGCTCTGCTTGGTTATCATGTTACGCCTCTTTTCTCGTACTATTCTTATCACGGACCTGTGTTTAGAGTCATGCTGAGGGTTCACCGTGGGAAGCTACATGAGGACAGGAACTATGGTTTTGTCACGCACTGTAATCTATGTGGCCATTCTCATACATTGAGATTCGATGAACTTGGTTTGATGGGATGTCCCTGCAGTGATACAAAGGCTTCTTCTTCACTGGTTGTGTCAGGACCAATGTGGCTCGGACCGCTTCATGATGCATCCTATGTTACGGAAATGCTGGAATTGGCAAAGGAATGGGGATGGGTCAGTGAAGGCACTGGCATGGATCTGGACAAGCTTCTCAGTATAATGATTGAAGAAAGTGATCCCAGACTACCACCTGGATACACAAAAATGGATGAGATGGCGAGCCGTGCGAAGATGAACTCACCTCCGTTGAAGAAGATGATGAGTGCACTAGTTAAGGAGGGTTACGCTGCTAGCAGATCTCACATTATTCCCAATGCTCTTAAAACAGATTGTCCCATGTCCCATTTTGTTAGGATTGCCAAGGAAAATTTGCATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

433

Amino Acids

48.47

Weight (kDa)

7.99

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 61 - 426 3.1e-62 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
AatII GACGTC 1 cut(s) 593
AccI GTMKAC 1 cut(s) 593
AciI CCGC 5 cut(s) 196, 291, 488, 694, 977
AclWI GGATC 2 cut(s) 1065, 1091
AcoI YGGCCR 1 cut(s) 868
AcsI RAATTY 3 cut(s) 129, 170, 1288
AcuI CTGAAG 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 590
AfaI GTAC 3 cut(s) 276, 415, 764
AfeI AGCGCT 1 cut(s) 208
AgsI TTSAA 6 cut(s) 103, 112, 152, 176, 1088, 1171
AhlI ACTAGT 1 cut(s) 1189
AjnI CCWGG 1 cut(s) 1111
AluBI AGCT 8 cut(s) 108, 267, 329, 335, 644, 725, 820, 1069
AluI AGCT 8 cut(s) 108, 267, 329, 335, 644, 725, 820, 1069
Alw21I GWGCWC 2 cut(s) 57, 1189
Alw26I GTCTC 2 cut(s) 179, 213
Alw44I GTGCAC 2 cut(s) 53, 1185
AlwI GGATC 2 cut(s) 1065, 1091
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 1 cut(s) 868
ApaLI GTGCAC 2 cut(s) 53, 1185
ApeKI GCWGC 4 cut(s) 641, 691, 725, 1208
ApoI RAATTY 3 cut(s) 129, 170, 1288
Asp700I GAANNNNTTC 1 cut(s) 529
AspLEI GCGC 1 cut(s) 209
AspS9I GGNCC 3 cut(s) 779, 959, 974
AsuHPI GGTGA 2 cut(s) 800, 1152
AsuNHI GCTAGC 1 cut(s) 1211
AvaII GGWCC 3 cut(s) 779, 959, 974
BaeGI GKGCMC 2 cut(s) 57, 1189
BalI TGGCCA 1 cut(s) 870
Bbv12I GWGCWC 2 cut(s) 57, 1189
BbvCI CCTCAGC 1 cut(s) 800
BbvI GCAGC 4 cut(s) 653, 678, 712, 1195
BccI CCATC 6 cut(s) 340, 426, 593, 901, 1026, 1129
BceAI ACGGC 2 cut(s) 655, 1128
BciT130I CCWGG 1 cut(s) 1113
BciVI GTATCC 1 cut(s) 1109
BcoDI GTCTC 2 cut(s) 179, 213
BcuI ACTAGT 1 cut(s) 1189
BfaI CTAG 3 cut(s) 210, 1190, 1212
BfmI CTRYAG 1 cut(s) 919
BfoI RGCGCY 1 cut(s) 210
BfuI GTATCC 1 cut(s) 1109
BglII AGATCT 2 cut(s) 254, 1217
BisI GCNGC 4 cut(s) 642, 692, 726, 1209
BlsI GCNGC 4 cut(s) 643, 693, 727, 1210
Bme1390I CCNGG 1 cut(s) 1113
Bme18I GGWCC 3 cut(s) 779, 959, 974
BmgT120I GGNCC 3 cut(s) 779, 959, 974
BmrFI CCNGG 1 cut(s) 1113
BmsI GCATC 4 cut(s) 538, 687, 976, 998
BmtI GCTAGC 1 cut(s) 1215
BpmI CTGGAG 2 cut(s) 69, 438
Bpu10I CCTNAGC 1 cut(s) 800
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 590
BsaI GGTCTC 1 cut(s) 213
BsaJI CCNNGG 4 cut(s) 144, 223, 811, 1281
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bse1I ACTGG 3 cut(s) 421, 951, 1054
Bse3DI GCAATG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 1113
BseDI CCNNGG 4 cut(s) 144, 223, 811, 1281
BseGI GGATG 6 cut(s) 299, 702, 917, 989, 1037, 1135
BseJI GATNNNNATC 1 cut(s) 369
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 791, 1087
BseNI ACTGG 3 cut(s) 421, 951, 1054
BseRI GAGGAG 1 cut(s) 442
BseSI GKGCMC 2 cut(s) 57, 1189
BseXI GCAGC 4 cut(s) 653, 678, 712, 1195
BshFI GGCC 1 cut(s) 870
BsiHKAI GWGCWC 2 cut(s) 57, 1189
BslFI GGGAC 3 cut(s) 900, 1239, 1246
BsmAI GTCTC 2 cut(s) 179, 213
BsmFI GGGAC 3 cut(s) 900, 1239, 1246
BsnI GGCC 1 cut(s) 870
Bso31I GGTCTC 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 57, 1189
Bsp143I GATC 5 cut(s) 124, 254, 1057, 1096, 1217
BspACI CCGC 5 cut(s) 196, 291, 488, 694, 977
BspANI GGCC 1 cut(s) 870
BspCNI CTCAG 2 cut(s) 792, 1086
BspHI TCATGA 1 cut(s) 982
BspMAI CTGCAG 1 cut(s) 923
BspOI GCTAGC 1 cut(s) 1215
BspPI GGATC 2 cut(s) 1065, 1091
BspTNI GGTCTC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 291
BsrI ACTGG 3 cut(s) 421, 951, 1054
BssECI CCNNGG 4 cut(s) 144, 223, 811, 1281
BssMI GATC 5 cut(s) 124, 254, 1057, 1096, 1217
BssNI GRCGYC 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 1281
Bst2UI CCWGG 1 cut(s) 1113
Bst4CI ACNGT 3 cut(s) 52, 812, 857
Bst6I CTCTTC 1 cut(s) 613
BstACI GRCGYC 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 1141, 1213
BstDEI CTNAG 2 cut(s) 800, 1073
BstDSI CCRYGG 1 cut(s) 811
BstF5I GGATG 6 cut(s) 299, 702, 917, 989, 1037, 1135
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 5 cut(s) 127, 257, 1060, 1099, 1220
BstMAI GTCTC 2 cut(s) 179, 213
BstMBI GATC 5 cut(s) 124, 254, 1057, 1096, 1217
BstMWI GCNNNNNNNGC 9 cut(s) 61, 204, 356, 461, 557, 650, 697, 976, 1145
BstNI CCWGG 1 cut(s) 1113
BstSCI CCNGG 1 cut(s) 1111
BstSFI CTRYAG 1 cut(s) 919
BstSLI GKGCMC 2 cut(s) 57, 1189
BstV1I GCAGC 4 cut(s) 653, 678, 712, 1195
BstX2I RGATCY 3 cut(s) 254, 1057, 1217
BstYI RGATCY 3 cut(s) 254, 1057, 1217
BsuI GTATCC 1 cut(s) 1109
BsuRI GGCC 1 cut(s) 870
BtgI CCRYGG 1 cut(s) 811
BtgZI GCGATG 1 cut(s) 479
BtsCI GGATG 6 cut(s) 299, 702, 917, 989, 1037, 1135
BtsI GCAGTG 2 cut(s) 720, 928
BtsIMutI CAGTG 7 cut(s) 48, 720, 853, 928, 944, 1045, 1047
Cac8I GCNNGC 2 cut(s) 1141, 1213
CciI TCATGA 1 cut(s) 982
CfoI GCGC 1 cut(s) 209
Cfr13I GGNCC 3 cut(s) 779, 959, 974
CpoI CGGWCCG 1 cut(s) 974
CseI GACGC 3 cut(s) 250, 290, 365
Csp6I GTAC 3 cut(s) 275, 414, 763
CspI CGGWCCG 1 cut(s) 974
CviAII CATG 8 cut(s) 93, 200, 743, 796, 824, 983, 1054, 1258
CviQI GTAC 3 cut(s) 275, 414, 763
DdeI CTNAG 2 cut(s) 800, 1073
DpnI GATC 5 cut(s) 126, 256, 1059, 1098, 1219
DpnII GATC 5 cut(s) 124, 254, 1057, 1096, 1217
EaeI YGGCCR 1 cut(s) 868
Eam1104I CTCTTC 1 cut(s) 613
EarI CTCTTC 1 cut(s) 613
EciI GGCGGA 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 1281
Eco31I GGTCTC 1 cut(s) 213
Eco47I GGWCC 3 cut(s) 779, 959, 974
Eco47III AGCGCT 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 1111
EcoT14I CCWWGG 1 cut(s) 1281
EcoT22I ATGCAT 2 cut(s) 562, 991
ErhI CCWWGG 1 cut(s) 1281
FaeI CATG 8 cut(s) 96, 203, 746, 799, 827, 986, 1057, 1261
FalI AAGNNNNNCTT 2 cut(s) 517, 549
FaqI GGGAC 3 cut(s) 900, 1239, 1246
FatI CATG 8 cut(s) 92, 199, 742, 795, 823, 982, 1053, 1257
FauNDI CATATG 1 cut(s) 447
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 4 cut(s) 642, 692, 726, 1209
FokI GGATG 6 cut(s) 306, 709, 924, 976, 1044, 1142
Fsp4HI GCNGC 4 cut(s) 642, 692, 726, 1209
FspBI CTAG 3 cut(s) 210, 1190, 1212
GlaI GCGC 1 cut(s) 208
GluI GCNGC 4 cut(s) 642, 692, 726, 1209
GsuI CTGGAG 2 cut(s) 69, 438
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 1 cut(s) 870
HgaI GACGC 3 cut(s) 250, 290, 365
HhaI GCGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 590
Hin1II CATG 8 cut(s) 96, 203, 746, 799, 827, 986, 1057, 1261
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 2 cut(s) 9, 594
HindII GTYRAC 2 cut(s) 9, 594
HindIII AAGCTT 2 cut(s) 265, 1067
HinfI GANTC 9 cut(s) 10, 89, 99, 163, 442, 515, 530, 792, 888
HphI GGTGA 2 cut(s) 800, 1152
Hpy166II GTNNAC 5 cut(s) 9, 55, 594, 808, 1187
Hpy188I TCNGA 3 cut(s) 145, 375, 974
Hpy188III TCNNGA 7 cut(s) 86, 103, 167, 343, 957, 983, 1061
Hpy8I GTNNAC 5 cut(s) 9, 55, 594, 808, 1187
Hpy99I CGWCG 4 cut(s) 244, 284, 359, 595
HpyAV CCTTC 2 cut(s) 52, 1037
HpyCH4III ACNGT 3 cut(s) 52, 812, 857
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 6 cut(s) 55, 560, 921, 989, 1187, 1294
HpyF10VI GCNNNNNNNGC 9 cut(s) 61, 204, 356, 461, 557, 650, 697, 976, 1145
HpyF3I CTNAG 2 cut(s) 800, 1073
HpySE526I ACGT 1 cut(s) 590
Hsp92I GRCGYC 1 cut(s) 590
Hsp92II CATG 8 cut(s) 96, 203, 746, 799, 827, 986, 1057, 1261
HspAI GCGC 1 cut(s) 207
Kzo9I GATC 5 cut(s) 124, 254, 1057, 1096, 1217
LmnI GCTCC 2 cut(s) 650, 710
Lsp1109I GCAGC 4 cut(s) 653, 678, 712, 1195
LweI GCATC 4 cut(s) 538, 687, 976, 998
MaeI CTAG 3 cut(s) 210, 1190, 1212
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 7 cut(s) 439, 602, 617, 745, 847, 997, 1202
MalI GATC 5 cut(s) 126, 256, 1059, 1098, 1219
MboI GATC 5 cut(s) 124, 254, 1057, 1096, 1217
MflI RGATCY 3 cut(s) 254, 1057, 1217
MhlI GDGCHC 2 cut(s) 57, 1189
MlsI TGGCCA 1 cut(s) 870
MluCI AATT 8 cut(s) 129, 153, 170, 300, 391, 631, 1013, 1288
MluNI TGGCCA 1 cut(s) 870
MlyI GAGTC 3 cut(s) 4, 436, 801
Mox20I TGGCCA 1 cut(s) 870
Mph1103I ATGCAT 2 cut(s) 562, 991
MroXI GAANNNNTTC 1 cut(s) 529
MscI TGGCCA 1 cut(s) 870
MseI TTAA 4 cut(s) 555, 1194, 1242, 1300
MslI CAYNNNNRTG 2 cut(s) 1052, 1124
Msp20I TGGCCA 1 cut(s) 870
MspR9I CCNGG 1 cut(s) 1113
MvaI CCWGG 1 cut(s) 1113
MwoI GCNNNNNNNGC 9 cut(s) 61, 204, 356, 461, 557, 650, 697, 976, 1145
NdeI CATATG 1 cut(s) 447
NdeII GATC 5 cut(s) 124, 254, 1057, 1096, 1217
NheI GCTAGC 1 cut(s) 1211
NlaIII CATG 8 cut(s) 96, 203, 746, 799, 827, 986, 1057, 1261
NmuCI GTSAC 3 cut(s) 439, 617, 847
NsiI ATGCAT 2 cut(s) 562, 991
PagI TCATGA 1 cut(s) 982
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 6 cut(s) 89, 99, 163, 515, 530, 888
PkrI GCNGC 4 cut(s) 643, 693, 727, 1210
PleI GAGTC 3 cut(s) 4, 436, 800
PpsI GAGTC 3 cut(s) 4, 436, 800
PsiI TTATAA 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 1111
PspGI CCWGG 1 cut(s) 1111
PspPI GGNCC 3 cut(s) 779, 959, 974
PstI CTGCAG 1 cut(s) 923
PsuI RGATCY 3 cut(s) 254, 1057, 1217
RsaI GTAC 3 cut(s) 276, 415, 764
RsaNI GTAC 3 cut(s) 275, 414, 763
RseI CAYNNNNRTG 2 cut(s) 1052, 1124
Rsr2I CGGWCCG 1 cut(s) 974
RsrII CGGWCCG 1 cut(s) 974
SalI GTCGAC 1 cut(s) 592
SaqAI TTAA 4 cut(s) 555, 1194, 1242, 1300
SatI GCNGC 4 cut(s) 642, 692, 726, 1209
Sau3AI GATC 5 cut(s) 124, 254, 1057, 1096, 1217
Sau96I GGNCC 3 cut(s) 779, 959, 974
SchI GAGTC 3 cut(s) 4, 436, 801
ScrFI CCNGG 1 cut(s) 1113
SduI GDGCHC 2 cut(s) 57, 1189
SfaNI GCATC 4 cut(s) 538, 687, 976, 998
SfcI CTRYAG 1 cut(s) 919
SinI GGWCC 3 cut(s) 779, 959, 974
SmiMI CAYNNNNRTG 2 cut(s) 1052, 1124
SpeI ACTAGT 1 cut(s) 1189
Sse9I AATT 8 cut(s) 129, 153, 170, 300, 391, 631, 1013, 1288
SsiI CCGC 5 cut(s) 196, 291, 488, 694, 977
SspMI CTAG 3 cut(s) 210, 1190, 1212
StyD4I CCNGG 1 cut(s) 1111
StyI CCWWGG 1 cut(s) 1281
TaaI ACNGT 3 cut(s) 52, 812, 857
TaiI ACGT 1 cut(s) 593
TaqI TCGA 8 cut(s) 97, 127, 168, 279, 498, 593, 661, 891
TasI AATT 8 cut(s) 129, 153, 170, 300, 391, 631, 1013, 1288
TfiI GAWTC 6 cut(s) 89, 99, 163, 515, 530, 888
Tru1I TTAA 4 cut(s) 555, 1194, 1242, 1300
Tru9I TTAA 4 cut(s) 555, 1194, 1242, 1300
TscAI CASTG 7 cut(s) 55, 720, 860, 928, 951, 1045, 1054
TseFI GTSAC 3 cut(s) 439, 617, 847
TseI GCWGC 4 cut(s) 641, 691, 725, 1208
Tsp45I GTSAC 3 cut(s) 439, 617, 847
TspDTI ATGAA 5 cut(s) 81, 216, 909, 971, 1169
TspGWI ACGGA 4 cut(s) 401, 792, 1016, 1155
TspRI CASTG 7 cut(s) 55, 720, 860, 928, 951, 1045, 1054
VneI GTGCAC 2 cut(s) 53, 1185
VpaK11BI GGWCC 3 cut(s) 779, 959, 974
XapI RAATTY 3 cut(s) 129, 170, 1288
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 1 cut(s) 529
XspI CTAG 3 cut(s) 210, 1190, 1212
ZraI GACGTC 1 cut(s) 591
Zsp2I ATGCAT 2 cut(s) 562, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.