Rw1G033210

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009588
Physical Location & Seq
Reverse (-)
61329072 .. 61333222
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033210.1

Sequence Viewer

Length: 1269 bp
ATGTTGTTGGCTCTGGCTCCTAAAACCCTATCCCCTTTCTCATTTCTCCCCAACCCAAAACCAAACCCCAAAAACCAAATCCCCAAATTCGCAACCCTAAACCAGAATCCAGTCCCCAATTCGGAGCCGATTGATCGAAAATGCGAGTATCGAACCGAGAGAGGTCTCAAATTCGACACGGGAGAGACATTTTTCCGGCACGAGAGCGCCACCGGCCGGGATTTGGGTGTTCTGGCCGCGACGCTGTACAAGAAATCTAGAGGGAAATTGCGTGTACTCGATGCCCTCTGCGGCTGCGGCATTCGGTCTCTGCGGTACCTGGTGGAGGCGGAGGCCGATTTTGTGTTGGCGAATGATGGAAATGTTGAGAGCAGGGACGTGATCACGGAGAACTTGAGGAGCTCCGGCGAGGAGGAGGAGGAGGAGAGGTGGAAGGTGACGCTTATGGATGCGAATCGGATCATGACCGAGTGTTATCTGAACAGGGATTTCTATGACCTCATTGACATTGATTCGTTTGGCAGCGACTCGTCGTTTCTCAGGTCGGCGATTAATGCTTTGAGGATGGATGGCTTGCTTTACGTTACCTCCACCGATGGTTTCTCTTCCGGGGGCCGCCGGCCGCAACAAACTTTAGCGGCATATGGAGCTTATGTTCGGCCTATGCCATATTCGAATGAGCTTGGTTTGAGAATGCTTATAGGAGGGGTGGTGCGGGTAGCTTCTGTGATGGGGTATCGTGTTACTCCGCTGTTTTCATACTATTCGTATCACGGACCCGTTTTCAGAGTCATGCTACGTGTCATTCGTGGAAAGCTCCCTGATAGCAGACACTACGGTTTTATCAGCTACTGCCGCGACTGTGGAAATTCTCATGAATTTTCATGGGATGAACTTGGTCGGATCAGTTGCCCCTGCAGTGATTCAAAGGTTTCTATGTCACTAGTTGTTTCTGGTCCTCTCTGGACAGGGCCTCTTCATGATGCTGCTTACATTACAGATATGTTAACTTTAGCAAAGGAATGGGGATGGGCGGGCAGTGACACAGGAACTGATCTTGACAAACTTTTAAATACAATGGTGGCTGAAAGCGACCCCAGATTACCGTTTGGCTTCATCAACTTGGACGAGGTAGCAAGCCGTGCAAAAGTAAATTCACCAGCACTCAAAGCTGTGGTGTCTGCCCTTCAAAAGGTAATCCCACATATTATCAACATATATCCATGGCCACATACTGCCATTGACACGTGCAAAGAATCAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

422

Amino Acids

47.15

Weight (kDa)

6.05

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 61 - 398 3.9e-54 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 315
AccII CGCG 2 cut(s) 239, 858
AclWI GGATC 2 cut(s) 467, 911
AcoI YGGCCR 4 cut(s) 214, 234, 620, 1226
AcsI RAATTY 5 cut(s) 86, 170, 868, 878, 1153
AcvI CACGTG 1 cut(s) 1248
AfaI GTAC 3 cut(s) 248, 276, 317
AfiI CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1192
AflIII ACRYGT 2 cut(s) 799, 1245
AgsI TTSAA 2 cut(s) 927, 1190
AhlI ACTAGT 1 cut(s) 943
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 1 cut(s) 318
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
AluBI AGCT 7 cut(s) 402, 650, 682, 722, 817, 849, 1172
AluI AGCT 7 cut(s) 402, 650, 682, 722, 817, 849, 1172
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 3 cut(s) 170, 179, 312
AlwI GGATC 2 cut(s) 467, 911
AlwNI CAGNNNCTG 2 cut(s) 852, 1052
AoxI GGCC 8 cut(s) 214, 234, 333, 613, 620, 659, 971, 1226
ApeKI GCWGC 3 cut(s) 294, 522, 986
ApoI RAATTY 5 cut(s) 86, 170, 868, 878, 1153
AseI ATTAAT 1 cut(s) 552
Asp718I GGTACC 1 cut(s) 315
AspLEI GCGC 1 cut(s) 209
AspS9I GGNCC 4 cut(s) 613, 776, 956, 971
AsuC2I CCSGG 2 cut(s) 218, 610
AsuHPI GGTGA 2 cut(s) 448, 1149
AsuII TTCGAA 1 cut(s) 674
AvaII GGWCC 2 cut(s) 776, 956
BalI TGGCCA 1 cut(s) 1228
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 404
BauI CACGAG 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 1248
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 3 cut(s) 281, 534, 973
BccI CCATC 6 cut(s) 350, 559, 563, 590, 724, 1023
BceAI ACGGC 1 cut(s) 1125
BciT130I CCWGG 1 cut(s) 320
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 2 cut(s) 218, 610
BcoDI GTCTC 3 cut(s) 170, 179, 312
BcuI ACTAGT 1 cut(s) 943
BfaI CTAG 2 cut(s) 258, 944
BfmI CTRYAG 1 cut(s) 916
BfoI RGCGCY 1 cut(s) 210
Bme1390I CCNGG 3 cut(s) 218, 320, 610
Bme18I GGWCC 2 cut(s) 776, 956
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 4 cut(s) 613, 776, 956, 971
BmiI GGNNCC 5 cut(s) 18, 126, 317, 614, 778
BmrFI CCNGG 3 cut(s) 218, 320, 610
BmsI GCATC 3 cut(s) 271, 439, 973
Bpu14I TTCGAA 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 415
BpuMI CCSGG 2 cut(s) 218, 610
BsaAI YACGTR 2 cut(s) 800, 1248
BsaBI GATNNNNATC 1 cut(s) 453
BsaI GGTCTC 2 cut(s) 170, 312
BsaJI CCNNGG 2 cut(s) 609, 1223
BsaXI ACNNNNNCTCC 8 cut(s) 413, 443, 572, 602, 639, 669, 696, 726
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1192
Bse118I RCCGGY 2 cut(s) 212, 618
Bse1I ACTGG 1 cut(s) 110
Bse8I GATNNNNATC 1 cut(s) 453
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 609, 1223
BseGI GGATG 5 cut(s) 454, 570, 574, 895, 1034
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1192
BseMII CTCAG 1 cut(s) 553
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 6 cut(s) 412, 425, 428, 431, 434, 437
BseX3I CGGCCG 2 cut(s) 214, 620
BseXI GCAGC 3 cut(s) 281, 534, 973
Bsh1236I CGCG 2 cut(s) 239, 858
Bsh1285I CGRYCG 2 cut(s) 217, 623
BshFI GGCC 8 cut(s) 216, 236, 335, 615, 622, 661, 973, 1228
BshNI GGYRCC 1 cut(s) 315
BsiEI CGRYCG 2 cut(s) 217, 623
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 6 cut(s) 196, 213, 217, 405, 609, 619
BslFI GGGAC 2 cut(s) 98, 389
BslI CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1192
BsmAI GTCTC 3 cut(s) 170, 179, 312
BsmFI GGGAC 2 cut(s) 98, 389
BsmI GAATGC 2 cut(s) 300, 699
BsnI GGCC 8 cut(s) 216, 236, 335, 615, 622, 661, 973, 1228
Bso31I GGTCTC 2 cut(s) 170, 312
Bsp119I TTCGAA 1 cut(s) 674
Bsp1286I GDGCHC 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 5 cut(s) 133, 381, 459, 903, 1054
Bsp19I CCATGG 1 cut(s) 1223
BspANI GGCC 8 cut(s) 216, 236, 335, 615, 622, 661, 973, 1228
BspCNI CTCAG 1 cut(s) 552
BspFNI CGCG 2 cut(s) 239, 858
BspHI TCATGA 3 cut(s) 462, 874, 979
BspLI GGNNCC 5 cut(s) 18, 126, 317, 614, 778
BspMAI CTGCAG 1 cut(s) 920
BspPI GGATC 2 cut(s) 467, 911
BspT104I TTCGAA 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 2 cut(s) 170, 312
BsrFI RCCGGY 2 cut(s) 212, 618
BsrGI TGTACA 1 cut(s) 246
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 2 cut(s) 212, 618
BssECI CCNNGG 2 cut(s) 609, 1223
BssMI GATC 5 cut(s) 133, 381, 459, 903, 1054
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 1223
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 3 cut(s) 839, 863, 1107
Bst6I CTCTTC 2 cut(s) 610, 981
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstAUI TGTACA 1 cut(s) 246
BstBAI YACGTR 2 cut(s) 800, 1248
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 575, 620, 1036, 1138
BstDEI CTNAG 1 cut(s) 539
BstDSI CCRYGG 1 cut(s) 1223
BstENI CCTNNNNNAGG 2 cut(s) 323, 1190
BstF5I GGATG 5 cut(s) 454, 570, 574, 895, 1034
BstFNI CGCG 2 cut(s) 239, 858
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 5 cut(s) 136, 384, 462, 906, 1057
BstMAI GTCTC 3 cut(s) 170, 179, 312
BstMBI GATC 5 cut(s) 133, 381, 459, 903, 1054
BstMCI CGRYCG 2 cut(s) 217, 623
BstMWI GCNNNNNNNGC 7 cut(s) 213, 297, 554, 647, 855, 1142, 1169
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 3 cut(s) 216, 318, 608
BstSFI CTRYAG 1 cut(s) 916
BstUI CGCG 2 cut(s) 239, 858
BstV1I GCAGC 3 cut(s) 281, 534, 973
BstZI CGGCCG 2 cut(s) 214, 620
BsuRI GGCC 8 cut(s) 216, 236, 335, 615, 622, 661, 973, 1228
BtgI CCRYGG 1 cut(s) 1223
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 5 cut(s) 454, 570, 574, 895, 1034
BtsI GCAGTG 2 cut(s) 925, 1045
BtsIMutI CAGTG 2 cut(s) 925, 1045
Cac8I GCNNGC 4 cut(s) 575, 620, 1036, 1138
CaiI CAGNNNCTG 2 cut(s) 852, 1052
CciI TCATGA 3 cut(s) 462, 874, 979
CfoI GCGC 1 cut(s) 209
Cfr10I RCCGGY 2 cut(s) 212, 618
Cfr13I GGNCC 4 cut(s) 613, 776, 956, 971
CseI GACGC 2 cut(s) 250, 448
CsiI ACCWGGT 1 cut(s) 318
Csp6I GTAC 3 cut(s) 247, 275, 316
CviAII CATG 6 cut(s) 463, 793, 875, 885, 980, 1224
CviQI GTAC 3 cut(s) 247, 275, 316
DdeI CTNAG 1 cut(s) 539
DpnI GATC 5 cut(s) 135, 383, 461, 905, 1056
DpnII GATC 5 cut(s) 133, 381, 459, 903, 1054
DraI TTTAAA 1 cut(s) 1071
EaeI YGGCCR 4 cut(s) 214, 234, 620, 1226
EagI CGGCCG 2 cut(s) 214, 620
Eam1104I CTCTTC 2 cut(s) 610, 981
EarI CTCTTC 2 cut(s) 610, 981
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 402
EclXI CGGCCG 2 cut(s) 214, 620
Eco130I CCWWGG 1 cut(s) 1223
Eco24I GRGCYC 1 cut(s) 404
Eco31I GGTCTC 2 cut(s) 170, 312
Eco47I GGWCC 2 cut(s) 776, 956
Eco52I CGGCCG 2 cut(s) 214, 620
Eco53kI GAGCTC 1 cut(s) 402
Eco72I CACGTG 1 cut(s) 1248
EcoICRI GAGCTC 1 cut(s) 402
EcoNI CCTNNNNNAGG 2 cut(s) 323, 1190
EcoO109I RGGNCCY 1 cut(s) 971
EcoRII CCWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 1223
EcoT38I GRGCYC 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 1223
FaeI CATG 6 cut(s) 466, 796, 878, 888, 983, 1227
FaqI GGGAC 2 cut(s) 98, 389
FatI CATG 6 cut(s) 462, 792, 874, 884, 979, 1223
FauI CCCGC 2 cut(s) 708, 1027
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 1 cut(s) 381
FokI GGATG 5 cut(s) 461, 577, 581, 902, 1041
FriOI GRGCYC 1 cut(s) 404
FspBI CTAG 2 cut(s) 258, 944
GlaI GCGC 1 cut(s) 208
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 8 cut(s) 216, 236, 335, 615, 622, 661, 973, 1228
HapII CCGG 6 cut(s) 196, 213, 217, 405, 609, 619
HgaI GACGC 2 cut(s) 250, 448
HhaI GCGC 1 cut(s) 209
Hin1II CATG 6 cut(s) 466, 796, 878, 888, 983, 1227
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HinfI GANTC 7 cut(s) 106, 454, 512, 527, 789, 923, 1256
HpaI GTTAAC 1 cut(s) 1008
HpaII CCGG 6 cut(s) 196, 213, 217, 405, 609, 619
HphI GGTGA 2 cut(s) 448, 1149
Hpy166II GTNNAC 2 cut(s) 275, 1008
Hpy188I TCNGA 6 cut(s) 124, 459, 480, 788, 903, 1261
Hpy188III TCNNGA 6 cut(s) 258, 463, 875, 964, 980, 1058
Hpy8I GTNNAC 2 cut(s) 275, 1008
Hpy99I CGWCG 2 cut(s) 244, 535
HpyAV CCTTC 2 cut(s) 427, 1196
HpyCH4III ACNGT 3 cut(s) 839, 863, 1107
HpyCH4IV ACGT 4 cut(s) 378, 582, 799, 1247
HpyCH4V TGCA 3 cut(s) 918, 1145, 1251
HpyF10VI GCNNNNNNNGC 7 cut(s) 213, 297, 554, 647, 855, 1142, 1169
HpyF3I CTNAG 1 cut(s) 539
HpySE526I ACGT 4 cut(s) 378, 582, 799, 1247
Hsp92II CATG 6 cut(s) 466, 796, 878, 888, 983, 1227
HspAI GCGC 1 cut(s) 207
KpnI GGTACC 1 cut(s) 319
KroI GCCGGC 1 cut(s) 618
KroNI GCCGGC 1 cut(s) 620
Ksp22I TGATCA 1 cut(s) 381
KspAI GTTAAC 1 cut(s) 1008
Kzo9I GATC 5 cut(s) 133, 381, 459, 903, 1054
LmnI GCTCC 6 cut(s) 22, 124, 399, 407, 647, 822
Lsp1109I GCAGC 3 cut(s) 281, 534, 973
LweI GCATC 3 cut(s) 271, 439, 973
MabI ACCWGGT 1 cut(s) 318
MaeI CTAG 2 cut(s) 258, 944
MaeII ACGT 4 cut(s) 378, 582, 799, 1247
MaeIII GTNAC 5 cut(s) 436, 583, 742, 939, 1040
MalI GATC 5 cut(s) 135, 383, 461, 905, 1056
MboI GATC 5 cut(s) 133, 381, 459, 903, 1054
MboII GAAGA 2 cut(s) 597, 968
MhlI GDGCHC 1 cut(s) 404
MlsI TGGCCA 1 cut(s) 1228
MluCI AATT 7 cut(s) 86, 118, 170, 266, 868, 878, 1153
MluNI TGGCCA 1 cut(s) 1228
MlyI GAGTC 2 cut(s) 521, 798
MmeI TCCRAC 1 cut(s) 881
Mox20I TGGCCA 1 cut(s) 1228
MroNI GCCGGC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 1228
MseI TTAA 3 cut(s) 552, 1007, 1070
Msp20I TGGCCA 1 cut(s) 1228
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 6 cut(s) 196, 213, 217, 405, 609, 619
MspR9I CCNGG 3 cut(s) 218, 320, 610
Mva1269I GAATGC 2 cut(s) 300, 699
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 2 cut(s) 239, 858
MwoI GCNNNNNNNGC 7 cut(s) 213, 297, 554, 647, 855, 1142, 1169
NaeI GCCGGC 1 cut(s) 620
NciI CCSGG 2 cut(s) 218, 610
NcoI CCATGG 1 cut(s) 1223
NdeI CATATG 1 cut(s) 643
NdeII GATC 5 cut(s) 133, 381, 459, 903, 1054
NgoMIV GCCGGC 1 cut(s) 618
NlaIII CATG 6 cut(s) 466, 796, 878, 888, 983, 1227
NlaIV GGNNCC 5 cut(s) 18, 126, 317, 614, 778
NmuCI GTSAC 3 cut(s) 436, 939, 1040
NspV TTCGAA 1 cut(s) 674
PagI TCATGA 3 cut(s) 462, 874, 979
PcsI WCGNNNNNNNCGW 1 cut(s) 805
PctI GAATGC 2 cut(s) 300, 699
PdiI GCCGGC 1 cut(s) 620
PfeI GAWTC 5 cut(s) 106, 454, 512, 923, 1256
PleI GAGTC 2 cut(s) 521, 797
PmaCI CACGTG 1 cut(s) 1248
PmlI CACGTG 1 cut(s) 1248
PpsI GAGTC 2 cut(s) 521, 797
Ppu21I YACGTR 2 cut(s) 800, 1248
PshBI ATTAAT 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 318
PspCI CACGTG 1 cut(s) 1248
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 5 cut(s) 18, 126, 317, 614, 778
PspPI GGNCC 4 cut(s) 613, 776, 956, 971
PstI CTGCAG 1 cut(s) 920
PstNI CAGNNNCTG 2 cut(s) 852, 1052
RsaI GTAC 3 cut(s) 248, 276, 317
RsaNI GTAC 3 cut(s) 247, 275, 316
SacI GAGCTC 1 cut(s) 404
SaqAI TTAA 3 cut(s) 552, 1007, 1070
Sau3AI GATC 5 cut(s) 133, 381, 459, 903, 1054
Sau96I GGNCC 4 cut(s) 613, 776, 956, 971
SchI GAGTC 2 cut(s) 521, 798
ScrFI CCNGG 3 cut(s) 218, 320, 610
SduI GDGCHC 1 cut(s) 404
SexAI ACCWGGT 1 cut(s) 318
SfaNI GCATC 3 cut(s) 271, 439, 973
SfcI CTRYAG 1 cut(s) 916
SfuI TTCGAA 1 cut(s) 674
SinI GGWCC 2 cut(s) 776, 956
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
SpeI ACTAGT 1 cut(s) 943
Sse9I AATT 7 cut(s) 86, 118, 170, 266, 868, 878, 1153
SspMI CTAG 2 cut(s) 258, 944
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 216, 318, 608
StyI CCWWGG 1 cut(s) 1223
TaaI ACNGT 3 cut(s) 839, 863, 1107
TaiI ACGT 4 cut(s) 381, 585, 802, 1250
TaqI TCGA 5 cut(s) 136, 151, 174, 279, 674
TaqII GACCGA 2 cut(s) 294, 482
TasI AATT 7 cut(s) 86, 118, 170, 266, 868, 878, 1153
TatI WGTACW 2 cut(s) 246, 274
TauI GCSGC 7 cut(s) 239, 294, 300, 618, 625, 641, 858
TfiI GAWTC 5 cut(s) 106, 454, 512, 923, 1256
Tru1I TTAA 3 cut(s) 552, 1007, 1070
Tru9I TTAA 3 cut(s) 552, 1007, 1070
TscAI CASTG 2 cut(s) 925, 1045
TseFI GTSAC 3 cut(s) 436, 939, 1040
TseI GCWGC 3 cut(s) 294, 522, 986
Tsp45I GTSAC 3 cut(s) 436, 939, 1040
TspDTI ATGAA 6 cut(s) 747, 873, 891, 906, 968, 1105
TspGWI ACGGA 2 cut(s) 401, 789
TspRI CASTG 2 cut(s) 925, 1045
VpaK11BI GGWCC 2 cut(s) 776, 956
VspI ATTAAT 1 cut(s) 552
XagI CCTNNNNNAGG 2 cut(s) 323, 1190
XapI RAATTY 5 cut(s) 86, 170, 868, 878, 1153
XbaI TCTAGA 1 cut(s) 257
XspI CTAG 2 cut(s) 258, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.