Rorug01G0367700

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009588
Physical Location & Seq
Forward (+)
48305797 .. 48307888
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0367700.1

Sequence Viewer

Length: 627 bp
ATGGCTGGTAATGAGATTGAAGAGTGGGTACCGAGAACGGAAGAAGGAGGTGATGAAATCCCCAATTACGGACGACGTCGTGCATCCATCGGCGCCTCCGCCTTTGCTCTCCCTGGTGGCCACCTCGAGTTCGGGAAGAATTATCTTCAGTGGCATGGTACTCGGGCTGAGTTGGTGGTGACGGAAGCCGAGTTGTGCAATGAGATACTCAGTAATAAAGATGGATTCTATCCGAAACCCAAGTTCAGAGCTTATGCAAAGAAGATATTTGGGAAAGGCCTTCCACAATTACAAGGTGAAAACTGGGCGAAATCGCGAATGCTGGCCAACCATGCCTTCCATGGACAGAGCTTAAAAGCTAAAGGTACCAAAAAGCCTCCTCTGACAAAGAATGAGCTTAGGGCCAAAGTTATACAGAAAGCAGAAAAGCTGAAGAATAAGAAGGATGCACATAAGGCAGCAGCTTTTGCTGCTGCAAAAACAACCACTGCTAAAGCAGCAACAAAGTCGAGCACTGCTTCAACCACTACACAAGCTGCTAGAGCAAACAGTAAAGCTTCTACCTCTGCAAAGTCTTCAACACCAACAAGATCATCACAGAGGATTAGAGCTGGAAAGGAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

208

Amino Acids

22.55

Weight (kDa)

10.17

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 79
Acc65I GGTACC 2 cut(s) 28, 365
AccB1I GGYRCC 3 cut(s) 28, 92, 365
AccII CGCG 1 cut(s) 316
AciI CCGC 1 cut(s) 99
AcoI YGGCCR 2 cut(s) 118, 324
AcuI CTGAAG 2 cut(s) 131, 452
AcyI GRCGYC 2 cut(s) 76, 93
AfaI GTAC 3 cut(s) 30, 160, 367
AfiI CCNNNNNNNGG 1 cut(s) 68
AgsI TTSAA 3 cut(s) 20, 522, 579
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 9 cut(s) 251, 351, 359, 397, 430, 464, 536, 557, 611
AluI AGCT 9 cut(s) 251, 351, 359, 397, 430, 464, 536, 557, 611
Alw21I GWGCWC 1 cut(s) 515
Ama87I CYCGRG 2 cut(s) 125, 162
AoxI GGCC 4 cut(s) 118, 277, 324, 402
ApeKI GCWGC 6 cut(s) 458, 461, 470, 473, 497, 536
Asp718I GGTACC 2 cut(s) 28, 365
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 402
AsuHPI GGTGA 3 cut(s) 62, 190, 308
AvaI CYCGRG 2 cut(s) 125, 162
BalI TGGCCA 2 cut(s) 120, 326
BanI GGYRCC 3 cut(s) 28, 92, 365
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 515
BbvI GCAGC 6 cut(s) 457, 460, 470, 473, 509, 523
BccI CCATC 2 cut(s) 95, 215
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 489, 523
BciT130I CCWGG 1 cut(s) 114
BfaI CTAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 6 cut(s) 459, 462, 471, 474, 498, 537
BlsI GCNGC 6 cut(s) 460, 463, 472, 475, 499, 538
Bme1390I CCNGG 1 cut(s) 114
BmeT110I CYCGRG 2 cut(s) 125, 162
BmgT120I GGNCC 1 cut(s) 402
BmiI GGNNCC 3 cut(s) 30, 94, 367
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 2 cut(s) 92, 436
BmuI ACTGGG 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 398
BsaHI GRCGYC 2 cut(s) 76, 93
BsaJI CCNNGG 2 cut(s) 112, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 112, 340
BseGI GGATG 2 cut(s) 83, 451
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 159, 223
BseNI ACTGG 1 cut(s) 308
BseRI GAGGAG 1 cut(s) 369
BseXI GCAGC 6 cut(s) 457, 460, 470, 473, 509, 523
Bsh1236I CGCG 1 cut(s) 316
BshFI GGCC 4 cut(s) 120, 279, 326, 404
BshNI GGYRCC 3 cut(s) 28, 92, 365
BsiHKAI GWGCWC 1 cut(s) 515
BsiHKCI CYCGRG 2 cut(s) 125, 162
BslI CCNNNNNNNGG 1 cut(s) 68
BsmI GAATGC 1 cut(s) 324
BsnI GGCC 4 cut(s) 120, 279, 326, 404
BsoBI CYCGRG 2 cut(s) 125, 162
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 1 cut(s) 590
Bsp19I CCATGG 1 cut(s) 340
Bsp68I TCGCGA 1 cut(s) 316
BspACI CCGC 1 cut(s) 99
BspANI GGCC 4 cut(s) 120, 279, 326, 404
BspCNI CTCAG 2 cut(s) 160, 222
BspFNI CGCG 1 cut(s) 316
BspLI GGNNCC 3 cut(s) 30, 94, 367
BspT107I GGYRCC 3 cut(s) 28, 92, 365
BsrDI GCAATG 1 cut(s) 205
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 112, 340
BssMI GATC 1 cut(s) 590
BssNI GRCGYC 2 cut(s) 76, 93
BssT1I CCWWGG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 2 cut(s) 76, 93
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 3 cut(s) 168, 209, 398
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 2 cut(s) 83, 451
BstFNI CGCG 1 cut(s) 316
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 1 cut(s) 593
BstMBI GATC 1 cut(s) 590
BstMWI GCNNNNNNNGC 6 cut(s) 332, 455, 467, 470, 497, 542
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstUI CGCG 1 cut(s) 316
BstV1I GCAGC 6 cut(s) 457, 460, 470, 473, 509, 523
BstV2I GAAGAC 1 cut(s) 567
BsuRI GGCC 4 cut(s) 120, 279, 326, 404
BtgI CCRYGG 1 cut(s) 340
BtsCI GGATG 2 cut(s) 83, 451
BtsI GCAGTG 2 cut(s) 486, 513
BtsIMutI CAGTG 3 cut(s) 155, 486, 513
BtuMI TCGCGA 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 402
Csp6I GTAC 3 cut(s) 29, 159, 366
CviAII CATG 4 cut(s) 155, 332, 341, 624
CviQI GTAC 3 cut(s) 29, 159, 366
DdeI CTNAG 3 cut(s) 168, 209, 398
DinI GGCGCC 1 cut(s) 94
DpnI GATC 1 cut(s) 592
DpnII GATC 1 cut(s) 590
EaeI YGGCCR 2 cut(s) 118, 324
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 340
Eco147I AGGCCT 1 cut(s) 279
Eco57I CTGAAG 2 cut(s) 131, 452
Eco88I CYCGRG 2 cut(s) 125, 162
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 340
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 4 cut(s) 158, 335, 344, 627
FaiI YATR 7 cut(s) 156, 255, 333, 342, 413, 453, 625
FatI CATG 4 cut(s) 154, 331, 340, 623
Fnu4HI GCNGC 6 cut(s) 459, 462, 471, 474, 498, 537
FokI GGATG 2 cut(s) 70, 458
Fsp4HI GCNGC 6 cut(s) 459, 462, 471, 474, 498, 537
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 94
GluI GCNGC 6 cut(s) 459, 462, 471, 474, 498, 537
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 120, 279, 326, 404
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 2 cut(s) 76, 93
Hin1II CATG 4 cut(s) 158, 335, 344, 627
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 1 cut(s) 225
HphI GGTGA 3 cut(s) 62, 190, 308
Hpy188I TCNGA 3 cut(s) 234, 248, 384
Hpy188III TCNNGA 2 cut(s) 133, 315
Hpy99I CGWCG 2 cut(s) 78, 81
HpyAV CCTTC 4 cut(s) 38, 290, 346, 436
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 6 cut(s) 83, 198, 257, 449, 476, 569
HpyF10VI GCNNNNNNNGC 6 cut(s) 332, 455, 467, 470, 497, 542
HpyF3I CTNAG 3 cut(s) 168, 209, 398
HpySE526I ACGT 1 cut(s) 76
Hsp92I GRCGYC 2 cut(s) 76, 93
Hsp92II CATG 4 cut(s) 158, 335, 344, 627
HspAI GCGC 1 cut(s) 93
KasI GGCGCC 1 cut(s) 92
KpnI GGTACC 2 cut(s) 32, 369
Kzo9I GATC 1 cut(s) 590
LpnPI CCDG 5 cut(s) 99, 126, 289, 308, 597
Lsp1109I GCAGC 6 cut(s) 457, 460, 470, 473, 509, 523
LweI GCATC 2 cut(s) 92, 436
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 1 cut(s) 178
MalI GATC 1 cut(s) 592
MboI GATC 1 cut(s) 590
MboII GAAGA 7 cut(s) 32, 53, 137, 148, 274, 445, 567
MhlI GDGCHC 1 cut(s) 515
MlsI TGGCCA 2 cut(s) 120, 326
MluCI AATT 3 cut(s) 64, 139, 287
MluNI TGGCCA 2 cut(s) 120, 326
Mly113I GGCGCC 1 cut(s) 93
MnlI CCTC 8 cut(s) 41, 106, 134, 387, 390, 574, 594, 613
Mox20I TGGCCA 2 cut(s) 120, 326
MscI TGGCCA 2 cut(s) 120, 326
MseI TTAA 1 cut(s) 353
Msp20I TGGCCA 2 cut(s) 120, 326
MspR9I CCNGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 316
MwoI GCNNNNNNNGC 6 cut(s) 332, 455, 467, 470, 497, 542
NarI GGCGCC 1 cut(s) 93
NcoI CCATGG 1 cut(s) 340
NdeII GATC 1 cut(s) 590
NlaIII CATG 4 cut(s) 158, 335, 344, 627
NlaIV GGNNCC 3 cut(s) 30, 94, 367
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 178
NruI TCGCGA 1 cut(s) 316
PaeR7I CTCGAG 1 cut(s) 125
PceI AGGCCT 1 cut(s) 279
PctI GAATGC 1 cut(s) 324
PfeI GAWTC 1 cut(s) 225
PflFI GACNNNGTC 1 cut(s) 75
PkrI GCNGC 6 cut(s) 460, 463, 472, 475, 499, 538
PluTI GGCGCC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 3 cut(s) 30, 94, 367
PspPI GGNCC 1 cut(s) 402
PspXI VCTCGAGB 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 75
RruI TCGCGA 1 cut(s) 316
RsaI GTAC 3 cut(s) 30, 160, 367
RsaNI GTAC 3 cut(s) 29, 159, 366
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 6 cut(s) 459, 462, 471, 474, 498, 537
Sau3AI GATC 1 cut(s) 590
Sau96I GGNCC 1 cut(s) 402
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 515
SfaNI GCATC 2 cut(s) 92, 436
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 3 cut(s) 64, 139, 287
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 1 cut(s) 99
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 1 cut(s) 540
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 551
TaiI ACGT 1 cut(s) 79
TaqI TCGA 2 cut(s) 126, 509
TasI AATT 3 cut(s) 64, 139, 287
TfiI GAWTC 1 cut(s) 225
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TscAI CASTG 3 cut(s) 155, 493, 520
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 6 cut(s) 458, 461, 470, 473, 497, 536
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 1 cut(s) 69
TspGWI ACGGA 3 cut(s) 53, 84, 197
TspRI CASTG 3 cut(s) 155, 493, 520
Tth111I GACNNNGTC 1 cut(s) 75
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XhoI CTCGAG 1 cut(s) 125
XspI CTAG 1 cut(s) 540
ZraI GACGTC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.