MD01G1164900.v1.1

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009588
Physical Location & Seq
Reverse (-)
26917768 .. 26921371
3604 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1164900.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGATAACTCTCTCTTCGCCTAAAACCCTATCTCCTTTCTCATTTCTCTGCAACCCCATCCCAAAACCCCAAATCCAAAACCCTAAATTCGCCGCGGTGACCCAATACTCAGACTCCAATTCGAAGCCAACCCTCGATTCCGATTGTGAATCGGTGTTCCAGACAGAGAGGGGTCTTAAATTCGACGTCGGCGGGACCTTCTTCCGCCACGAAAGCGCCACCGGCCGCGACCTCGGCGTGCTCGCCGCCGCTCTGTACAAGAAGTCCGAGGGCAAATTGCGAGTTCTCGATGCACTGTGTGGCTGCGGGATTCGGTCTGTTCGGTACCTCGTCGAGGCGGAGGCCGATTTTGTTCTGGCAAATGACGCGAATGATGAGAACAAGAAAGTTATCACGGAGAACTTGTCGCAGGTGGAGAGTGGTTCCGGGGATGAGGCGAGGTGGGAGGTGTCGCTTACGGATGCGAATCGGATCATGACGGAGTGTTATCTGAAAAGGGATTTCTTTGATTTGATTGACATTGATTCGTTTGGGAGCGATTCGTCGTTTTTGAGGTCGGCGTTTAATGCTTTGAAGTTGAATGGATTGTTGTATGTTACTTCAACTGATGGGTTCTCGTCCGGTGGTCGCAGGCCTCAACAAACTTTAGCTGCATATGGAGCTTATGTCCGGCCAATGCCATACTCGAATGAGCTTGGTTTGCGAATGCTTATTGGAGGGGCTGTGCGGGAAGCTTCAGTGCTGGGGTATCGAGTTACTCCACTGTTTTCATACTATTCATATCATGGACCAATTTTCAGAGTCATGCTCCAAGTCAATCGGGGAAAGCTTCCTGATAACAAACATTATGGGTTCATCAGCTACTGCAACAGCTGTGGAAGTTCTCAAGAGTACTCATGGGCTGAACTTGGTCGGATCGGTTGCCCCTGCAGTGATTCAAAGGTCTCCAAGTCACTTGTTGTTTCTGGCCCTCTCTGGACAGGGCCGTTGCATGACACTGCTTATATTACAGAAATGTTAAGTTTGGCCGAGGAATGGGGATGGGCAGGCATTGAAACAGGAACTGATCTGGACAAACTTTTGAACCAAATGGTGGCTGAGAGTGACCCCAGATTACCCTTTGGCTACACCAAGTTGGACGAGGTGGCAAGTCGTGCGAAAGTAAATTCTCCGTCACTGAGAGCCGTGATGTCCACCCTTCAAAAGGAAGGATATGCTGTGAGTAGGTCACACATTGAATCCAATGCAATCAAGACGAATTGTCCGATGGAAGACTGCATTAGAATTGCCAAGCAACTTCAGCAGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

437

Amino Acids

48.15

Weight (kDa)

6.06

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 68 - 429 1.7e-65 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
AatII GACGTC 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 400
Acc65I GGTACC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 324
AccB7I CCANNNNNTGG 1 cut(s) 1093
AccBSI CCGCTC 1 cut(s) 251
AccII CGCG 3 cut(s) 95, 228, 368
AclWI GGATC 2 cut(s) 479, 923
AcoI YGGCCR 3 cut(s) 223, 671, 1026
AcsI RAATTY 3 cut(s) 86, 179, 1165
AcuI CTGAAG 2 cut(s) 720, 1283
AcyI GRCGYC 1 cut(s) 186
AdeI CACNNNGTG 1 cut(s) 299
AfaI GTAC 3 cut(s) 257, 326, 893
AfeI AGCGCT 1 cut(s) 1307
AfiI CCNNNNNNNGG 4 cut(s) 334, 1035, 1093, 1204
AgsI TTSAA 8 cut(s) 574, 580, 603, 939, 1055, 1084, 1202, 1238
AhdI GACNNNNNGTC 1 cut(s) 1260
AjuI GAANNNNNNNTTGG 2 cut(s) 121, 153
AluBI AGCT 7 cut(s) 650, 662, 694, 734, 829, 861, 873
AluI AGCT 7 cut(s) 650, 662, 694, 734, 829, 861, 873
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 949
AlwI GGATC 2 cut(s) 479, 923
AlwNI CAGNNNCTG 2 cut(s) 864, 1064
Aor51HI AGCGCT 1 cut(s) 1307
AoxI GGCC 7 cut(s) 223, 342, 632, 671, 967, 983, 1026
ApeKI GCWGC 3 cut(s) 303, 650, 1303
ApoI RAATTY 3 cut(s) 86, 179, 1165
Asp718I GGTACC 1 cut(s) 324
AspLEI GCGC 2 cut(s) 218, 1308
AspS9I GGNCC 4 cut(s) 195, 788, 968, 983
AsuC2I CCSGG 1 cut(s) 427
AsuHPI GGTGA 1 cut(s) 109
AsuII TTCGAA 1 cut(s) 122
AvaII GGWCC 2 cut(s) 195, 788
BanI GGYRCC 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 1278
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 290, 637
BccI CCATC 4 cut(s) 65, 602, 1035, 1261
BceAI ACGGC 2 cut(s) 970, 1169
BcnI CCSGG 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 949
BfmI CTRYAG 1 cut(s) 928
BfoI RGCGCY 2 cut(s) 219, 1309
BfuAI ACCTGC 1 cut(s) 400
BisI GCNGC 7 cut(s) 93, 226, 246, 249, 304, 651, 1304
BlsI GCNGC 7 cut(s) 94, 227, 247, 250, 305, 652, 1305
BmcAI AGTACT 1 cut(s) 893
Bme1390I CCNGG 1 cut(s) 427
Bme18I GGWCC 2 cut(s) 195, 788
BmeRI GACNNNNNGTC 1 cut(s) 1260
BmgT120I GGNCC 4 cut(s) 195, 788, 968, 983
BmiI GGNNCC 3 cut(s) 196, 326, 424
BmrFI CCNGG 1 cut(s) 427
BmsI GCATC 2 cut(s) 280, 451
BpiI GAAGAC 1 cut(s) 1278
BplI GAGNNNNNCTC 2 cut(s) 792, 824
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 870
BpuMI CCSGG 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 949
BsaJI CCNNGG 5 cut(s) 93, 232, 267, 426, 1029
BsaWI WCCGGW 1 cut(s) 620
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 1035, 1093, 1204
Bse118I RCCGGY 1 cut(s) 221
Bse8I GATNNNNATC 1 cut(s) 465
BseDI CCNNGG 5 cut(s) 93, 232, 267, 426, 1029
BseGI GGATG 4 cut(s) 57, 436, 466, 1046
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 4 cut(s) 334, 1035, 1093, 1204
BseMII CTCAG 3 cut(s) 123, 1089, 1169
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 2 cut(s) 290, 637
BseYI CCCAGC 1 cut(s) 742
Bsh1236I CGCG 3 cut(s) 95, 228, 368
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
BshNI GGYRCC 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 4 cut(s) 222, 426, 621, 670
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 4 cut(s) 334, 1035, 1093, 1204
BsmAI GTCTC 1 cut(s) 949
BsmFI GGGAC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 711
BsnI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
Bso31I GGTCTC 1 cut(s) 949
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 243
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 3 cut(s) 471, 915, 1066
BspANI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
BspCNI CTCAG 3 cut(s) 122, 1090, 1170
BspFNI CGCG 3 cut(s) 95, 228, 368
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 196, 326, 424
BspMAI CTGCAG 1 cut(s) 932
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 2 cut(s) 479, 923
BspT104I TTCGAA 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 949
BsrBI CCGCTC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 5 cut(s) 93, 232, 267, 426, 1029
BssMI GATC 3 cut(s) 471, 915, 1066
BssNI GRCGYC 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 297, 765
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstAUI TGTACA 1 cut(s) 255
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 4 cut(s) 239, 243, 632, 1048
BstDEI CTNAG 3 cut(s) 109, 1098, 1178
BstDSI CCRYGG 1 cut(s) 93
BstEII GGTNACC 1 cut(s) 97
BstENI CCTNNNNNAGG 2 cut(s) 332, 1202
BstF5I GGATG 4 cut(s) 57, 436, 466, 1046
BstFNI CGCG 3 cut(s) 95, 228, 368
BstH2I RGCGCY 2 cut(s) 219, 1309
BstHHI GCGC 2 cut(s) 218, 1308
BstKTI GATC 3 cut(s) 474, 918, 1069
BstMAI GTCTC 1 cut(s) 949
BstMBI GATC 3 cut(s) 471, 915, 1066
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 9 cut(s) 213, 222, 234, 365, 566, 659, 700, 1154, 1300
BstPI GGTNACC 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 425
BstSFI CTRYAG 1 cut(s) 928
BstUI CGCG 3 cut(s) 95, 228, 368
BstV1I GCAGC 2 cut(s) 290, 637
BstV2I GAAGAC 1 cut(s) 1278
BstZI CGGCCG 1 cut(s) 223
BsuRI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 4 cut(s) 57, 436, 466, 1046
BtsI GCAGTG 2 cut(s) 937, 996
BtsIMutI CAGTG 6 cut(s) 293, 744, 761, 937, 996, 1175
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 4 cut(s) 239, 243, 632, 1048
CaiI CAGNNNCTG 2 cut(s) 864, 1064
CciI TCATGA 1 cut(s) 474
CfoI GCGC 2 cut(s) 218, 1308
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 4 cut(s) 195, 788, 968, 983
Cfr42I CCGCGG 1 cut(s) 96
CseI GACGC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 256, 325, 892
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 5 cut(s) 475, 785, 805, 897, 992
CviQI GTAC 3 cut(s) 256, 325, 892
DdeI CTNAG 3 cut(s) 109, 1098, 1178
DpnI GATC 3 cut(s) 473, 917, 1068
DpnII GATC 3 cut(s) 471, 915, 1066
DraIII CACNNNGTG 1 cut(s) 299
DriI GACNNNNNGTC 1 cut(s) 1260
EaeI YGGCCR 3 cut(s) 223, 671, 1026
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 1260
EarI CTCTTC 1 cut(s) 19
EciI GGCGGA 2 cut(s) 194, 353
EclXI CGGCCG 1 cut(s) 223
Eco147I AGGCCT 1 cut(s) 634
Eco31I GGTCTC 1 cut(s) 949
Eco47I GGWCC 2 cut(s) 195, 788
Eco47III AGCGCT 1 cut(s) 1307
Eco52I CGGCCG 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 720, 1283
Eco91I GGTNACC 1 cut(s) 97
EcoNI CCTNNNNNAGG 2 cut(s) 332, 1202
EcoO109I RGGNCCY 1 cut(s) 195
EcoO65I GGTNACC 1 cut(s) 97
FaeI CATG 5 cut(s) 478, 788, 808, 900, 995
FaqI GGGAC 1 cut(s) 208
FatI CATG 5 cut(s) 474, 784, 804, 896, 991
FauI CCCGC 3 cut(s) 185, 299, 720
FauNDI CATATG 1 cut(s) 655
Fnu4HI GCNGC 7 cut(s) 93, 226, 246, 249, 304, 651, 1304
FokI GGATG 4 cut(s) 44, 443, 473, 1053
Fsp4HI GCNGC 7 cut(s) 93, 226, 246, 249, 304, 651, 1304
GlaI GCGC 2 cut(s) 217, 1307
GluI GCNGC 7 cut(s) 93, 226, 246, 249, 304, 651, 1304
GsaI CCCAGC 1 cut(s) 746
HaeII RGCGCY 2 cut(s) 219, 1309
HaeIII GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
HapII CCGG 4 cut(s) 222, 426, 621, 670
HgaI GACGC 1 cut(s) 374
HhaI GCGC 2 cut(s) 218, 1308
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 5 cut(s) 478, 788, 808, 900, 995
Hin6I GCGC 2 cut(s) 216, 1306
HinP1I GCGC 2 cut(s) 216, 1306
HindIII AAGCTT 2 cut(s) 732, 827
HpaII CCGG 4 cut(s) 222, 426, 621, 670
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 1194
Hpy188I TCNGA 8 cut(s) 112, 142, 268, 471, 492, 800, 915, 1266
Hpy188III TCNNGA 8 cut(s) 160, 287, 475, 833, 887, 976, 1070, 1252
Hpy8I GTNNAC 1 cut(s) 1194
Hpy99I CGWCG 4 cut(s) 188, 191, 335, 547
HpyAV CCTTC 3 cut(s) 208, 1202, 1208
HpyCH4III ACNGT 2 cut(s) 297, 765
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 8 cut(s) 51, 293, 653, 867, 930, 991, 1247, 1278
HpyF10VI GCNNNNNNNGC 9 cut(s) 213, 222, 234, 365, 566, 659, 700, 1154, 1300
HpyF3I CTNAG 3 cut(s) 109, 1098, 1178
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 5 cut(s) 478, 788, 808, 900, 995
HspAI GCGC 2 cut(s) 216, 1306
KpnI GGTACC 1 cut(s) 328
KspI CCGCGG 1 cut(s) 96
Kzo9I GATC 3 cut(s) 471, 915, 1066
LmnI GCTCC 3 cut(s) 534, 659, 813
Lsp1109I GCAGC 2 cut(s) 290, 637
LweI GCATC 2 cut(s) 280, 451
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 7 cut(s) 97, 595, 754, 951, 1103, 1173, 1227
MalI GATC 3 cut(s) 473, 917, 1068
MbiI CCGCTC 1 cut(s) 251
MboI GATC 3 cut(s) 471, 915, 1066
MboII GAAGA 3 cut(s) 6, 193, 1283
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 8 cut(s) 86, 118, 179, 275, 792, 1165, 1258, 1284
MlyI GAGTC 2 cut(s) 107, 810
MmeI TCCRAC 2 cut(s) 893, 1116
MseI TTAA 3 cut(s) 177, 564, 1019
MspA1I CMGCKG 2 cut(s) 95, 873
MspI CCGG 4 cut(s) 222, 426, 621, 670
MspR9I CCNGG 1 cut(s) 427
Mva1269I GAATGC 1 cut(s) 711
MvnI CGCG 3 cut(s) 95, 228, 368
MwoI GCNNNNNNNGC 9 cut(s) 213, 222, 234, 365, 566, 659, 700, 1154, 1300
NciI CCSGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 655
NdeII GATC 3 cut(s) 471, 915, 1066
NlaIII CATG 5 cut(s) 478, 788, 808, 900, 995
NlaIV GGNNCC 3 cut(s) 196, 326, 424
NmeAIII GCCGAG 2 cut(s) 213, 1054
NmuCI GTSAC 5 cut(s) 97, 951, 1103, 1173, 1227
NspV TTCGAA 1 cut(s) 122
PagI TCATGA 1 cut(s) 474
PaqCI CACCTGC 1 cut(s) 400
PceI AGGCCT 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 1262
PctI GAATGC 1 cut(s) 711
PfeI GAWTC 8 cut(s) 137, 149, 310, 466, 524, 539, 935, 1238
PflMI CCANNNNNTGG 1 cut(s) 1093
PkrI GCNGC 7 cut(s) 94, 227, 247, 250, 305, 652, 1305
PleI GAGTC 2 cut(s) 107, 809
PpsI GAGTC 2 cut(s) 107, 809
PpuMI RGGWCCY 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 195
PspEI GGTNACC 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 742
PspN4I GGNNCC 3 cut(s) 196, 326, 424
PspPI GGNCC 4 cut(s) 195, 788, 968, 983
PspPPI RGGWCCY 1 cut(s) 195
PstI CTGCAG 1 cut(s) 932
PstNI CAGNNNCTG 2 cut(s) 864, 1064
PvuII CAGCTG 1 cut(s) 873
RsaI GTAC 3 cut(s) 257, 326, 893
RsaNI GTAC 3 cut(s) 256, 325, 892
SacII CCGCGG 1 cut(s) 96
SaqAI TTAA 3 cut(s) 177, 564, 1019
SatI GCNGC 7 cut(s) 93, 226, 246, 249, 304, 651, 1304
Sau3AI GATC 3 cut(s) 471, 915, 1066
Sau96I GGNCC 4 cut(s) 195, 788, 968, 983
ScaI AGTACT 1 cut(s) 893
SchI GAGTC 2 cut(s) 107, 810
ScrFI CCNGG 1 cut(s) 427
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 2 cut(s) 280, 451
SfcI CTRYAG 1 cut(s) 928
Sfr303I CCGCGG 1 cut(s) 96
SfuI TTCGAA 1 cut(s) 122
SgrBI CCGCGG 1 cut(s) 96
SinI GGWCC 2 cut(s) 195, 788
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
Sse9I AATT 8 cut(s) 86, 118, 179, 275, 792, 1165, 1258, 1284
SseBI AGGCCT 1 cut(s) 634
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 1 cut(s) 425
TaaI ACNGT 2 cut(s) 297, 765
TaiI ACGT 1 cut(s) 189
TaqI TCGA 7 cut(s) 122, 135, 183, 288, 333, 686, 751
TaqII GACCGA 1 cut(s) 303
TasI AATT 8 cut(s) 86, 118, 179, 275, 792, 1165, 1258, 1284
TatI WGTACW 2 cut(s) 255, 891
TauI GCSGC 4 cut(s) 95, 228, 248, 251
TfiI GAWTC 8 cut(s) 137, 149, 310, 466, 524, 539, 935, 1238
Tru1I TTAA 3 cut(s) 177, 564, 1019
Tru9I TTAA 3 cut(s) 177, 564, 1019
TscAI CASTG 6 cut(s) 300, 744, 768, 937, 1003, 1182
TseFI GTSAC 5 cut(s) 97, 951, 1103, 1173, 1227
TseI GCWGC 3 cut(s) 303, 650, 1303
Tsp45I GTSAC 5 cut(s) 97, 951, 1103, 1173, 1227
TspDTI ATGAA 3 cut(s) 759, 768, 844
TspGWI ACGGA 4 cut(s) 410, 473, 494, 1161
TspRI CASTG 6 cut(s) 300, 744, 768, 937, 1003, 1182
Van91I CCANNNNNTGG 1 cut(s) 1093
VpaK11BI GGWCC 2 cut(s) 195, 788
XagI CCTNNNNNAGG 2 cut(s) 332, 1202
XapI RAATTY 3 cut(s) 86, 179, 1165
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.