Rh1BG340900

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0009588
Physical Location & Seq
Reverse (-)
47459044 .. 47461555
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG340900.1

Sequence Viewer

Length: 1296 bp
ATGTTGTTGGCTCTCGCTCCTAAAACCCTATCCCCTTTCTCATTTCTCCCCAACCCAAAACCAAACCCCAAAAACCAAATCCCCAAATTCGCTACCCTAAACCAGAATCCAATCCCCAATTCTGAGCCGATTGATCGAAAATGCGAGTATCGAACCGAGAGAGGTCTCAAATTCGACACCGGAGAGACATTTTTCCGGCACGAGAGCGCCACCGGCCGGGATTTGGGTGTTCTGGCCGCGACGCTGTACAAGAAATCTAGAGGGAAATTGCGGGTACTCGATGCCCTCTGCGGCTGCGGCATTCGGTCTCTGCGGTACCTGGTGGAGGCGGAGGCCGATTTTGTGTTGGCGAATGATGGAAATGTTGAGAGCAGGGATGTGATCACGGAGAACTTGAGGAGCTCCGGCGAGGAGGAGGAGGAGGAGAGGTGGAAGGTGACGCTTATGGATGCGAATCGGATCATGACCGAGTGTTATCTGAACAGGGATTTCTATGACCTTATTGACATTGATTCGTTTGGCAGCGACTCGTCGTTTCTCAGGTCGGCGATTAATGCTTTGAGGATGGATGGCTTGCTTTACCTTACCTCCACCGATGGTTTCTCTTCCGGTGGCCGCCGGCCGCAACAAACTTTAGCGGCATATGGAGCTTATGTTCGGCCTATGCCATATTCGAATGAGCTTGGTTTGAGAATGCTTATAGGAGGGGTGGTGCGGGTAGCTTCTGTGATGGGGTATCGTGTTACTCCGTTGTTTTCATACTATTCGTATCATGGACCTGTTTTCAGAGTCATGCTACGTGTCATTCGTGGAAAGCTCCCTGATAGCAGACACTACGGTTTTATCAGCTACTGCCGTGACTGTGGAAATTCTCATGAATTTTCATGGGATGAACTTGGTCGGATCAGTTGCCCCTGCAGTGATTCAAAGGTTTCTATGTCACTAGTTGTTTCTGGTCCTCTCTGGACAGGGCCTCTTCATGATGCTGCTTACATTACAGACATGTTAACTTTAGCAAAGGAATGGAGATGGGCGGGCAGTGACACAGGAACTGATCTTGACAAACTTTTAAATACAATGGTGGCTGAGAGCGACCCCAGATTACCGTTCGGCTTCATCAACTTGGACGAGGTAGCAAGCCGTGCAAAAGTAAATTCACCAGCACTCAAAGCTGTGGTGTCTGCCCTTCAAAAGGAGGGATATGCTGTGAGTAGGTCACATATCGAGTCCAATGCACTCAAGACCAACTGTCCAATGTCAGAATGCATTAGAATTGCGCAAGAACTACAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

431

Amino Acids

48.17

Weight (kDa)

6.62

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 61 - 425 2.3e-64 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1278
Acc65I GGTACC 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 315
AccII CGCG 1 cut(s) 239
AclWI GGATC 2 cut(s) 467, 911
AcoI YGGCCR 4 cut(s) 214, 234, 613, 620
AcsI RAATTY 5 cut(s) 86, 170, 868, 878, 1153
AfaI GTAC 3 cut(s) 248, 276, 317
AfiI CCNNNNNNNGG 4 cut(s) 216, 223, 325, 1192
AflIII ACRYGT 2 cut(s) 799, 1002
AgsI TTSAA 2 cut(s) 927, 1190
AhdI GACNNNNNGTC 1 cut(s) 1248
AhlI ACTAGT 1 cut(s) 943
AjnI CCWGG 1 cut(s) 318
AjuI GAANNNNNNNTTGG 2 cut(s) 103, 135
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
AluBI AGCT 7 cut(s) 402, 650, 682, 722, 817, 849, 1172
AluI AGCT 7 cut(s) 402, 650, 682, 722, 817, 849, 1172
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 3 cut(s) 170, 179, 312
AlwI GGATC 2 cut(s) 467, 911
AlwNI CAGNNNCTG 2 cut(s) 852, 1052
AoxI GGCC 7 cut(s) 214, 234, 333, 613, 620, 659, 971
ApeKI GCWGC 3 cut(s) 294, 522, 986
ApoI RAATTY 5 cut(s) 86, 170, 868, 878, 1153
AseI ATTAAT 1 cut(s) 552
Asp718I GGTACC 1 cut(s) 315
AspLEI GCGC 2 cut(s) 209, 1279
AspS9I GGNCC 3 cut(s) 776, 956, 971
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 448, 1149
AsuII TTCGAA 1 cut(s) 674
AvaII GGWCC 2 cut(s) 776, 956
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 404
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 3 cut(s) 281, 534, 973
BccI CCATC 6 cut(s) 350, 559, 563, 590, 724, 1023
BceAI ACGGC 2 cut(s) 840, 1125
BcgI CGANNNNNNTGC 2 cut(s) 1214, 1248
BciT130I CCWGG 1 cut(s) 320
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 3 cut(s) 170, 179, 312
BcuI ACTAGT 1 cut(s) 943
BfaI CTAG 2 cut(s) 258, 944
BfmI CTRYAG 2 cut(s) 916, 1286
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 9 cut(s) 237, 292, 295, 298, 523, 616, 623, 639, 987
BlsI GCNGC 9 cut(s) 238, 293, 296, 299, 524, 617, 624, 640, 988
Bme1390I CCNGG 2 cut(s) 218, 320
Bme18I GGWCC 2 cut(s) 776, 956
BmeRI GACNNNNNGTC 1 cut(s) 1248
BmgT120I GGNCC 3 cut(s) 776, 956, 971
BmiI GGNNCC 1 cut(s) 317
BmrFI CCNGG 2 cut(s) 218, 320
BmsI GCATC 3 cut(s) 271, 439, 973
Bpu14I TTCGAA 1 cut(s) 674
BpuEI CTTGAG 2 cut(s) 415, 1223
BpuMI CCSGG 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 800
BsaBI GATNNNNATC 1 cut(s) 453
BsaI GGTCTC 2 cut(s) 170, 312
BsaWI WCCGGW 2 cut(s) 179, 608
BsaXI ACNNNNNCTCC 8 cut(s) 413, 443, 572, 602, 639, 669, 696, 726
Bsc4I CCNNNNNNNGG 4 cut(s) 216, 223, 325, 1192
Bse118I RCCGGY 2 cut(s) 212, 618
Bse8I GATNNNNATC 1 cut(s) 453
BseBI CCWGG 1 cut(s) 320
BseGI GGATG 5 cut(s) 382, 454, 570, 574, 895
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 4 cut(s) 216, 223, 325, 1192
BseMII CTCAG 3 cut(s) 114, 553, 1077
BseRI GAGGAG 6 cut(s) 412, 425, 428, 431, 434, 437
BseX3I CGGCCG 2 cut(s) 214, 620
BseXI GCAGC 3 cut(s) 281, 534, 973
Bsh1236I CGCG 1 cut(s) 239
Bsh1285I CGRYCG 2 cut(s) 217, 623
BshFI GGCC 7 cut(s) 216, 236, 335, 615, 622, 661, 973
BshNI GGYRCC 1 cut(s) 315
BsiEI CGRYCG 2 cut(s) 217, 623
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 7 cut(s) 180, 196, 213, 217, 405, 609, 619
BslI CCNNNNNNNGG 4 cut(s) 216, 223, 325, 1192
BsmAI GTCTC 3 cut(s) 170, 179, 312
BsmI GAATGC 3 cut(s) 300, 699, 1268
BsnI GGCC 7 cut(s) 216, 236, 335, 615, 622, 661, 973
Bso31I GGTCTC 2 cut(s) 170, 312
Bsp119I TTCGAA 1 cut(s) 674
Bsp1286I GDGCHC 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 5 cut(s) 133, 381, 459, 903, 1054
BspANI GGCC 7 cut(s) 216, 236, 335, 615, 622, 661, 973
BspCNI CTCAG 3 cut(s) 115, 552, 1078
BspFNI CGCG 1 cut(s) 239
BspHI TCATGA 3 cut(s) 462, 874, 979
BspLI GGNNCC 1 cut(s) 317
BspMAI CTGCAG 1 cut(s) 920
BspPI GGATC 2 cut(s) 467, 911
BspT104I TTCGAA 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 2 cut(s) 170, 312
BsrFI RCCGGY 2 cut(s) 212, 618
BsrGI TGTACA 1 cut(s) 246
BssAI RCCGGY 2 cut(s) 212, 618
BssMI GATC 5 cut(s) 133, 381, 459, 903, 1054
BssSI CACGAG 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 4 cut(s) 839, 863, 1107, 1250
Bst6I CTCTTC 2 cut(s) 610, 981
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstAUI TGTACA 1 cut(s) 246
BstBAI YACGTR 1 cut(s) 800
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 575, 620, 1036, 1138
BstDEI CTNAG 3 cut(s) 123, 539, 1086
BstENI CCTNNNNNAGG 2 cut(s) 323, 1190
BstF5I GGATG 5 cut(s) 382, 454, 570, 574, 895
BstFNI CGCG 1 cut(s) 239
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 2 cut(s) 209, 1279
BstKTI GATC 5 cut(s) 136, 384, 462, 906, 1057
BstMAI GTCTC 3 cut(s) 170, 179, 312
BstMBI GATC 5 cut(s) 133, 381, 459, 903, 1054
BstMCI CGRYCG 2 cut(s) 217, 623
BstMWI GCNNNNNNNGC 6 cut(s) 213, 297, 554, 647, 1142, 1169
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 1006
BstSCI CCNGG 2 cut(s) 216, 318
BstSFI CTRYAG 2 cut(s) 916, 1286
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 3 cut(s) 281, 534, 973
BstZI CGGCCG 2 cut(s) 214, 620
BsuRI GGCC 7 cut(s) 216, 236, 335, 615, 622, 661, 973
BtsCI GGATG 5 cut(s) 382, 454, 570, 574, 895
BtsI GCAGTG 2 cut(s) 925, 1045
BtsIMutI CAGTG 2 cut(s) 925, 1045
Cac8I GCNNGC 4 cut(s) 575, 620, 1036, 1138
CaiI CAGNNNCTG 2 cut(s) 852, 1052
CciI TCATGA 3 cut(s) 462, 874, 979
CfoI GCGC 2 cut(s) 209, 1279
Cfr10I RCCGGY 2 cut(s) 212, 618
Cfr13I GGNCC 3 cut(s) 776, 956, 971
CseI GACGC 2 cut(s) 250, 448
CsiI ACCWGGT 1 cut(s) 318
Csp6I GTAC 3 cut(s) 247, 275, 316
CviAII CATG 7 cut(s) 463, 773, 793, 875, 885, 980, 1003
CviQI GTAC 3 cut(s) 247, 275, 316
DdeI CTNAG 3 cut(s) 123, 539, 1086
DpnI GATC 5 cut(s) 135, 383, 461, 905, 1056
DpnII GATC 5 cut(s) 133, 381, 459, 903, 1054
DraI TTTAAA 1 cut(s) 1071
DriI GACNNNNNGTC 1 cut(s) 1248
EaeI YGGCCR 4 cut(s) 214, 234, 613, 620
EagI CGGCCG 2 cut(s) 214, 620
Eam1104I CTCTTC 2 cut(s) 610, 981
Eam1105I GACNNNNNGTC 1 cut(s) 1248
EarI CTCTTC 2 cut(s) 610, 981
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 402
EclXI CGGCCG 2 cut(s) 214, 620
Eco24I GRGCYC 1 cut(s) 404
Eco31I GGTCTC 2 cut(s) 170, 312
Eco47I GGWCC 2 cut(s) 776, 956
Eco52I CGGCCG 2 cut(s) 214, 620
Eco53kI GAGCTC 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 402
EcoNI CCTNNNNNAGG 2 cut(s) 323, 1190
EcoO109I RGGNCCY 1 cut(s) 971
EcoRII CCWGG 1 cut(s) 318
EcoT22I ATGCAT 1 cut(s) 1268
EcoT38I GRGCYC 1 cut(s) 404
FaeI CATG 7 cut(s) 466, 776, 796, 878, 888, 983, 1006
FatI CATG 7 cut(s) 462, 772, 792, 874, 884, 979, 1002
FauI CCCGC 3 cut(s) 264, 708, 1027
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 9 cut(s) 237, 292, 295, 298, 523, 616, 623, 639, 987
FokI GGATG 5 cut(s) 389, 461, 577, 581, 902
FriOI GRGCYC 1 cut(s) 404
Fsp4HI GCNGC 9 cut(s) 237, 292, 295, 298, 523, 616, 623, 639, 987
FspBI CTAG 2 cut(s) 258, 944
FspI TGCGCA 1 cut(s) 1278
GlaI GCGC 2 cut(s) 208, 1278
GluI GCNGC 9 cut(s) 237, 292, 295, 298, 523, 616, 623, 639, 987
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 7 cut(s) 216, 236, 335, 615, 622, 661, 973
HapII CCGG 7 cut(s) 180, 196, 213, 217, 405, 609, 619
HgaI GACGC 2 cut(s) 250, 448
HhaI GCGC 2 cut(s) 209, 1279
Hin1II CATG 7 cut(s) 466, 776, 796, 878, 888, 983, 1006
Hin6I GCGC 2 cut(s) 207, 1277
HinP1I GCGC 2 cut(s) 207, 1277
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HinfI GANTC 7 cut(s) 106, 454, 512, 527, 789, 923, 1226
HpaI GTTAAC 1 cut(s) 1008
HpaII CCGG 7 cut(s) 180, 196, 213, 217, 405, 609, 619
HphI GGTGA 2 cut(s) 448, 1149
Hpy166II GTNNAC 1 cut(s) 1008
Hpy188I TCNGA 6 cut(s) 124, 459, 480, 788, 903, 1261
Hpy188III TCNNGA 7 cut(s) 258, 463, 875, 964, 980, 1058, 1240
Hpy8I GTNNAC 1 cut(s) 1008
Hpy99I CGWCG 2 cut(s) 244, 535
HpyAV CCTTC 2 cut(s) 427, 1196
HpyCH4III ACNGT 4 cut(s) 839, 863, 1107, 1250
HpyCH4IV ACGT 1 cut(s) 799
HpyCH4V TGCA 4 cut(s) 918, 1145, 1235, 1266
HpyF10VI GCNNNNNNNGC 6 cut(s) 213, 297, 554, 647, 1142, 1169
HpyF3I CTNAG 3 cut(s) 123, 539, 1086
HpySE526I ACGT 1 cut(s) 799
Hsp92II CATG 7 cut(s) 466, 776, 796, 878, 888, 983, 1006
HspAI GCGC 2 cut(s) 207, 1277
KpnI GGTACC 1 cut(s) 319
KroI GCCGGC 1 cut(s) 618
KroNI GCCGGC 1 cut(s) 620
Ksp22I TGATCA 1 cut(s) 381
KspAI GTTAAC 1 cut(s) 1008
Kzo9I GATC 5 cut(s) 133, 381, 459, 903, 1054
LmnI GCTCC 5 cut(s) 22, 399, 407, 647, 822
Lsp1109I GCAGC 3 cut(s) 281, 534, 973
LweI GCATC 3 cut(s) 271, 439, 973
MabI ACCWGGT 1 cut(s) 318
MaeI CTAG 2 cut(s) 258, 944
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 6 cut(s) 436, 742, 857, 939, 1040, 1215
MalI GATC 5 cut(s) 135, 383, 461, 905, 1056
MboI GATC 5 cut(s) 133, 381, 459, 903, 1054
MboII GAAGA 2 cut(s) 597, 968
MhlI GDGCHC 1 cut(s) 404
MluCI AATT 8 cut(s) 86, 118, 170, 266, 868, 878, 1153, 1272
MlyI GAGTC 3 cut(s) 521, 798, 1235
MmeI TCCRAC 1 cut(s) 881
Mph1103I ATGCAT 1 cut(s) 1268
MroNI GCCGGC 1 cut(s) 618
MseI TTAA 3 cut(s) 552, 1007, 1070
MspI CCGG 7 cut(s) 180, 196, 213, 217, 405, 609, 619
MspR9I CCNGG 2 cut(s) 218, 320
Mva1269I GAATGC 3 cut(s) 300, 699, 1268
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 6 cut(s) 213, 297, 554, 647, 1142, 1169
NaeI GCCGGC 1 cut(s) 620
NciI CCSGG 1 cut(s) 218
NdeI CATATG 1 cut(s) 643
NdeII GATC 5 cut(s) 133, 381, 459, 903, 1054
NgoMIV GCCGGC 1 cut(s) 618
NlaIII CATG 7 cut(s) 466, 776, 796, 878, 888, 983, 1006
NlaIV GGNNCC 1 cut(s) 317
NmuCI GTSAC 5 cut(s) 436, 857, 939, 1040, 1215
NsbI TGCGCA 1 cut(s) 1278
NsiI ATGCAT 1 cut(s) 1268
NspI RCATGY 1 cut(s) 1006
NspV TTCGAA 1 cut(s) 674
PagI TCATGA 3 cut(s) 462, 874, 979
PciI ACATGT 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 805
PctI GAATGC 3 cut(s) 300, 699, 1268
PdiI GCCGGC 1 cut(s) 620
PfeI GAWTC 4 cut(s) 106, 454, 512, 923
PkrI GCNGC 9 cut(s) 238, 293, 296, 299, 524, 617, 624, 640, 988
PleI GAGTC 3 cut(s) 521, 797, 1234
PpsI GAGTC 3 cut(s) 521, 797, 1234
Ppu21I YACGTR 1 cut(s) 800
PscI ACATGT 1 cut(s) 1002
PshBI ATTAAT 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 317
PspPI GGNCC 3 cut(s) 776, 956, 971
PstI CTGCAG 1 cut(s) 920
PstNI CAGNNNCTG 2 cut(s) 852, 1052
RsaI GTAC 3 cut(s) 248, 276, 317
RsaNI GTAC 3 cut(s) 247, 275, 316
SacI GAGCTC 1 cut(s) 404
SaqAI TTAA 3 cut(s) 552, 1007, 1070
SatI GCNGC 9 cut(s) 237, 292, 295, 298, 523, 616, 623, 639, 987
Sau3AI GATC 5 cut(s) 133, 381, 459, 903, 1054
Sau96I GGNCC 3 cut(s) 776, 956, 971
SchI GAGTC 3 cut(s) 521, 798, 1235
ScrFI CCNGG 2 cut(s) 218, 320
SduI GDGCHC 1 cut(s) 404
SexAI ACCWGGT 1 cut(s) 318
SfaNI GCATC 3 cut(s) 271, 439, 973
SfcI CTRYAG 2 cut(s) 916, 1286
SfuI TTCGAA 1 cut(s) 674
SinI GGWCC 2 cut(s) 776, 956
SmlI CTYRAG 2 cut(s) 394, 1238
SmoI CTYRAG 2 cut(s) 394, 1238
SpeI ACTAGT 1 cut(s) 943
Sse9I AATT 8 cut(s) 86, 118, 170, 266, 868, 878, 1153, 1272
SspMI CTAG 2 cut(s) 258, 944
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 216, 318
TaaI ACNGT 4 cut(s) 839, 863, 1107, 1250
TaiI ACGT 1 cut(s) 802
TaqI TCGA 6 cut(s) 136, 151, 174, 279, 674, 1224
TaqII GACCGA 2 cut(s) 294, 482
TasI AATT 8 cut(s) 86, 118, 170, 266, 868, 878, 1153, 1272
TatI WGTACW 1 cut(s) 246
TauI GCSGC 6 cut(s) 239, 294, 300, 618, 625, 641
TfiI GAWTC 4 cut(s) 106, 454, 512, 923
Tru1I TTAA 3 cut(s) 552, 1007, 1070
Tru9I TTAA 3 cut(s) 552, 1007, 1070
TscAI CASTG 2 cut(s) 925, 1045
TseFI GTSAC 5 cut(s) 436, 857, 939, 1040, 1215
TseI GCWGC 3 cut(s) 294, 522, 986
Tsp45I GTSAC 5 cut(s) 436, 857, 939, 1040, 1215
TspDTI ATGAA 6 cut(s) 747, 873, 891, 906, 968, 1105
TspGWI ACGGA 2 cut(s) 401, 738
TspRI CASTG 2 cut(s) 925, 1045
VpaK11BI GGWCC 2 cut(s) 776, 956
VspI ATTAAT 1 cut(s) 552
XagI CCTNNNNNAGG 2 cut(s) 323, 1190
XapI RAATTY 5 cut(s) 86, 170, 868, 878, 1153
XbaI TCTAGA 1 cut(s) 257
XceI RCATGY 1 cut(s) 1006
XspI CTAG 2 cut(s) 258, 944
Zsp2I ATGCAT 1 cut(s) 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.