Rh1CG354000

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0009588
Physical Location & Seq
Reverse (-)
62946094 .. 62949087
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG354000.1

Sequence Viewer

Length: 1299 bp
ATGTTGTTGGCTCTCGCTCCTAAAACCCTATCCCCTTTCTCATTTCTAGTTCTCCCCAACCCAAAACCAAACCCCAAAAACCAAATCCCCAAATTCGCAACCCTAAACCAGAATCCAGTCCCCAATTCGGAGCCGATTGATCGAAAATCTGAGTTACGGACCGAACGGGGTCTCAAATTCGACACCGGAGAGACATTTTTCCGGCAAGAGAGCGCCACCGGCCGGGATTTGGGTGTTCTGGCCGCGACGCTGTACAAGAAATCTAGAGGGAAATTGCGTGTACTGGATGCGCTGTGCGGCTGCGGCATTCGGTCTCTGCGGTACCTGGTGGAGGCGGAGGCCGATTTTGTGTTGGCGAATGATGGAAATGTTGAGAGCAGAGATGTGATCACGGAGAACTTGAGGAGCTCCGGCGAGGAGGAGGAGGAGAGGTGGAAGGTGATGCTTATGGATGCGAATCGGATCATGACCGAGTGTTATCTGAACAGGGATTTCTATGACCTCATTGACATTGATTCGTTTGGCAGCGACTCGTCGTTTCTCAGGTCGGCGATTAATGCTTTGAGGATGGATGGCTTGCTTTACGTTACCTCCACCGATGGTTTCTCTTCCGGGGGCCGCCGGCCGCAACAAACTTTAGCGGCATATGGTGCTTATGTTCGGCCGATGCCATATTCGAATGAGCTTGGTTTGAGAATGCTTATAGGAGGGGTGGTGCGGGTAGCTTCTGTGATGGGGTATCGTGTTACTCCGCTGTTTTCATACTATTCGTATCACGGACCCGTTTTCAGAGTCATGCTACGTGTCATTCGTGGAAAGCTCCCTGATAGCAGACACTACGGTTTTATCAGCTACTGCCGCGACTGTGGAAATTCTCATGAATTTTCATGGGATGAACTTGGTCGGATCAGTTGCCCCTGCAGTGATTCAAAGGTTTCTATGTCACTAGTTGTTTCTGGTCCTCTCTGGACAGGGCCTCTTCATGATGCTGCTTACATTACAGATATGTTGACTTTAGCAAAGGAATGGGGATGGGCGGGCAGTGACACAGGAACTGATCTTGACAAACTTTTAAATACAATGGTGGCTGAGAGCGACCCCAGATTACCGTTTGGCTTCATCAACTTGGACGAGGTAGCAAGCCGTGCAAAAGTAAATTCACCAGCACTCAAAGCTGTGGTGTCTGCCCTTCAAAAGGAGGGATATGCTGTGAGTAGGTCACATATCGAGTCCAATGCACTCAAGACCAACTGTCCAATGTCAGAATGCATTAGAATTGCGCAAGAACTACAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

432

Amino Acids

48.09

Weight (kDa)

6.56

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 63 - 426 1.3e-64 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1281
Acc65I GGTACC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 321
AccII CGCG 2 cut(s) 245, 861
AclWI GGATC 2 cut(s) 470, 914
AcoI YGGCCR 4 cut(s) 220, 240, 623, 662
AcsI RAATTY 5 cut(s) 92, 176, 871, 881, 1156
AfaI GTAC 3 cut(s) 254, 282, 323
AfiI CCNNNNNNNGG 5 cut(s) 127, 222, 229, 331, 1195
AflIII ACRYGT 1 cut(s) 802
AgsI TTSAA 2 cut(s) 930, 1193
AhdI GACNNNNNGTC 1 cut(s) 1251
AhlI ACTAGT 1 cut(s) 946
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 6 cut(s) 408, 685, 725, 820, 852, 1175
AluI AGCT 6 cut(s) 408, 685, 725, 820, 852, 1175
Alw21I GWGCWC 1 cut(s) 410
Alw26I GTCTC 3 cut(s) 176, 185, 318
AlwI GGATC 2 cut(s) 470, 914
AlwNI CAGNNNCTG 2 cut(s) 855, 1055
AoxI GGCC 7 cut(s) 220, 240, 339, 616, 623, 662, 974
ApeKI GCWGC 3 cut(s) 300, 525, 989
ApoI RAATTY 5 cut(s) 92, 176, 871, 881, 1156
AseI ATTAAT 1 cut(s) 555
Asp718I GGTACC 1 cut(s) 321
AspLEI GCGC 3 cut(s) 215, 292, 1282
AspS9I GGNCC 5 cut(s) 159, 616, 779, 959, 974
AsuC2I CCSGG 2 cut(s) 224, 613
AsuHPI GGTGA 2 cut(s) 451, 1152
AsuII TTCGAA 1 cut(s) 677
AvaII GGWCC 3 cut(s) 159, 779, 959
BanI GGYRCC 1 cut(s) 321
BanII GRGCYC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 410
BbvI GCAGC 3 cut(s) 287, 537, 976
BccI CCATC 6 cut(s) 356, 562, 566, 593, 727, 1026
BceAI ACGGC 1 cut(s) 1128
BcgI CGANNNNNNTGC 2 cut(s) 1217, 1251
BciT130I CCWGG 1 cut(s) 326
BclI TGATCA 1 cut(s) 387
BcnI CCSGG 2 cut(s) 224, 613
BcoDI GTCTC 3 cut(s) 176, 185, 318
BcuI ACTAGT 1 cut(s) 946
BfaI CTAG 3 cut(s) 47, 264, 947
BfmI CTRYAG 2 cut(s) 919, 1289
BfoI RGCGCY 1 cut(s) 216
Bme1390I CCNGG 3 cut(s) 224, 326, 613
Bme18I GGWCC 3 cut(s) 159, 779, 959
BmeRI GACNNNNNGTC 1 cut(s) 1251
BmgT120I GGNCC 5 cut(s) 159, 616, 779, 959, 974
BmiI GGNNCC 4 cut(s) 132, 323, 617, 781
BmrFI CCNGG 3 cut(s) 224, 326, 613
BmsI GCATC 5 cut(s) 277, 432, 442, 657, 976
Bpu14I TTCGAA 1 cut(s) 677
BpuEI CTTGAG 2 cut(s) 421, 1226
BpuMI CCSGG 2 cut(s) 224, 613
BsaAI YACGTR 1 cut(s) 803
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 2 cut(s) 176, 318
BsaJI CCNNGG 1 cut(s) 612
BsaWI WCCGGW 1 cut(s) 185
BsaXI ACNNNNNCTCC 6 cut(s) 416, 446, 575, 605, 699, 729
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 222, 229, 331, 1195
Bse118I RCCGGY 2 cut(s) 218, 621
Bse1I ACTGG 2 cut(s) 116, 288
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 612
BseGI GGATG 6 cut(s) 292, 457, 573, 577, 898, 1037
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 5 cut(s) 127, 222, 229, 331, 1195
BseMII CTCAG 3 cut(s) 141, 556, 1080
BseNI ACTGG 2 cut(s) 116, 288
BseRI GAGGAG 5 cut(s) 418, 431, 434, 437, 440
BseX3I CGGCCG 3 cut(s) 220, 623, 662
BseXI GCAGC 3 cut(s) 287, 537, 976
Bsh1236I CGCG 2 cut(s) 245, 861
Bsh1285I CGRYCG 3 cut(s) 223, 626, 665
BshFI GGCC 7 cut(s) 222, 242, 341, 618, 625, 664, 976
BshNI GGYRCC 1 cut(s) 321
BsiEI CGRYCG 3 cut(s) 223, 626, 665
BsiHKAI GWGCWC 1 cut(s) 410
BsiSI CCGG 7 cut(s) 186, 202, 219, 223, 411, 612, 622
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 5 cut(s) 127, 222, 229, 331, 1195
BsmAI GTCTC 3 cut(s) 176, 185, 318
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 3 cut(s) 306, 702, 1271
BsnI GGCC 7 cut(s) 222, 242, 341, 618, 625, 664, 976
Bso31I GGTCTC 2 cut(s) 176, 318
Bsp119I TTCGAA 1 cut(s) 677
Bsp1286I GDGCHC 1 cut(s) 410
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 5 cut(s) 139, 387, 462, 906, 1057
BspANI GGCC 7 cut(s) 222, 242, 341, 618, 625, 664, 976
BspCNI CTCAG 3 cut(s) 142, 555, 1081
BspFNI CGCG 2 cut(s) 245, 861
BspHI TCATGA 3 cut(s) 465, 877, 982
BspLI GGNNCC 4 cut(s) 132, 323, 617, 781
BspMAI CTGCAG 1 cut(s) 923
BspPI GGATC 2 cut(s) 470, 914
BspT104I TTCGAA 1 cut(s) 677
BspT107I GGYRCC 1 cut(s) 321
BspTNI GGTCTC 2 cut(s) 176, 318
BsrFI RCCGGY 2 cut(s) 218, 621
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 2 cut(s) 116, 288
BssAI RCCGGY 2 cut(s) 218, 621
BssECI CCNNGG 1 cut(s) 612
BssMI GATC 5 cut(s) 139, 387, 462, 906, 1057
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 4 cut(s) 842, 866, 1110, 1253
Bst6I CTCTTC 2 cut(s) 613, 984
BstAPI GCANNNNNTGC 2 cut(s) 650, 1145
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 803
BstBI TTCGAA 1 cut(s) 677
BstC8I GCNNGC 4 cut(s) 578, 623, 1039, 1141
BstDEI CTNAG 3 cut(s) 150, 542, 1089
BstENI CCTNNNNNAGG 2 cut(s) 329, 1193
BstF5I GGATG 6 cut(s) 292, 457, 573, 577, 898, 1037
BstFNI CGCG 2 cut(s) 245, 861
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 3 cut(s) 215, 292, 1282
BstKTI GATC 5 cut(s) 142, 390, 465, 909, 1060
BstMAI GTCTC 3 cut(s) 176, 185, 318
BstMBI GATC 5 cut(s) 139, 387, 462, 906, 1057
BstMCI CGRYCG 3 cut(s) 223, 626, 665
BstMWI GCNNNNNNNGC 7 cut(s) 219, 303, 557, 650, 858, 1145, 1172
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 3 cut(s) 222, 324, 611
BstSFI CTRYAG 2 cut(s) 919, 1289
BstUI CGCG 2 cut(s) 245, 861
BstV1I GCAGC 3 cut(s) 287, 537, 976
BstZI CGGCCG 3 cut(s) 220, 623, 662
BsuRI GGCC 7 cut(s) 222, 242, 341, 618, 625, 664, 976
BtsCI GGATG 6 cut(s) 292, 457, 573, 577, 898, 1037
BtsI GCAGTG 2 cut(s) 928, 1048
BtsIMutI CAGTG 2 cut(s) 928, 1048
Cac8I GCNNGC 4 cut(s) 578, 623, 1039, 1141
CaiI CAGNNNCTG 2 cut(s) 855, 1055
CciI TCATGA 3 cut(s) 465, 877, 982
CfoI GCGC 3 cut(s) 215, 292, 1282
Cfr10I RCCGGY 2 cut(s) 218, 621
Cfr13I GGNCC 5 cut(s) 159, 616, 779, 959, 974
CpoI CGGWCCG 1 cut(s) 159
CseI GACGC 1 cut(s) 256
CsiI ACCWGGT 1 cut(s) 324
Csp6I GTAC 3 cut(s) 253, 281, 322
CspI CGGWCCG 1 cut(s) 159
CviAII CATG 5 cut(s) 466, 796, 878, 888, 983
CviQI GTAC 3 cut(s) 253, 281, 322
DdeI CTNAG 3 cut(s) 150, 542, 1089
DpnI GATC 5 cut(s) 141, 389, 464, 908, 1059
DpnII GATC 5 cut(s) 139, 387, 462, 906, 1057
DraI TTTAAA 1 cut(s) 1074
DriI GACNNNNNGTC 1 cut(s) 1251
EaeI YGGCCR 4 cut(s) 220, 240, 623, 662
EagI CGGCCG 3 cut(s) 220, 623, 662
Eam1104I CTCTTC 2 cut(s) 613, 984
Eam1105I GACNNNNNGTC 1 cut(s) 1251
EarI CTCTTC 2 cut(s) 613, 984
EciI GGCGGA 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 408
EclXI CGGCCG 3 cut(s) 220, 623, 662
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 2 cut(s) 176, 318
Eco47I GGWCC 3 cut(s) 159, 779, 959
Eco52I CGGCCG 3 cut(s) 220, 623, 662
Eco53kI GAGCTC 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 408
EcoNI CCTNNNNNAGG 2 cut(s) 329, 1193
EcoO109I RGGNCCY 1 cut(s) 974
EcoRII CCWGG 1 cut(s) 324
EcoT22I ATGCAT 1 cut(s) 1271
EcoT38I GRGCYC 1 cut(s) 410
FaeI CATG 5 cut(s) 469, 799, 881, 891, 986
FaqI GGGAC 1 cut(s) 104
FatI CATG 5 cut(s) 465, 795, 877, 887, 982
FauI CCCGC 2 cut(s) 711, 1030
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 387
FokI GGATG 6 cut(s) 299, 464, 580, 584, 905, 1044
FriOI GRGCYC 1 cut(s) 410
FspBI CTAG 3 cut(s) 47, 264, 947
FspI TGCGCA 1 cut(s) 1281
GlaI GCGC 3 cut(s) 214, 291, 1281
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 7 cut(s) 222, 242, 341, 618, 625, 664, 976
HapII CCGG 7 cut(s) 186, 202, 219, 223, 411, 612, 622
HgaI GACGC 1 cut(s) 256
HhaI GCGC 3 cut(s) 215, 292, 1282
Hin1II CATG 5 cut(s) 469, 799, 881, 891, 986
Hin6I GCGC 3 cut(s) 213, 290, 1280
HinP1I GCGC 3 cut(s) 213, 290, 1280
HincII GTYRAC 1 cut(s) 1011
HindII GTYRAC 1 cut(s) 1011
HinfI GANTC 7 cut(s) 112, 457, 515, 530, 792, 926, 1229
HpaII CCGG 7 cut(s) 186, 202, 219, 223, 411, 612, 622
HphI GGTGA 2 cut(s) 451, 1152
Hpy166II GTNNAC 2 cut(s) 281, 1011
Hpy188I TCNGA 7 cut(s) 130, 151, 462, 483, 791, 906, 1264
Hpy188III TCNNGA 7 cut(s) 264, 466, 878, 967, 983, 1061, 1243
Hpy8I GTNNAC 2 cut(s) 281, 1011
Hpy99I CGWCG 2 cut(s) 250, 538
HpyAV CCTTC 2 cut(s) 430, 1199
HpyCH4III ACNGT 4 cut(s) 842, 866, 1110, 1253
HpyCH4IV ACGT 2 cut(s) 585, 802
HpyCH4V TGCA 4 cut(s) 921, 1148, 1238, 1269
HpyF10VI GCNNNNNNNGC 7 cut(s) 219, 303, 557, 650, 858, 1145, 1172
HpyF3I CTNAG 3 cut(s) 150, 542, 1089
HpySE526I ACGT 2 cut(s) 585, 802
Hsp92II CATG 5 cut(s) 469, 799, 881, 891, 986
HspAI GCGC 3 cut(s) 213, 290, 1280
KpnI GGTACC 1 cut(s) 325
KroI GCCGGC 1 cut(s) 621
KroNI GCCGGC 1 cut(s) 623
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 5 cut(s) 139, 387, 462, 906, 1057
LmnI GCTCC 5 cut(s) 22, 130, 405, 413, 825
Lsp1109I GCAGC 3 cut(s) 287, 537, 976
LweI GCATC 5 cut(s) 277, 432, 442, 657, 976
MabI ACCWGGT 1 cut(s) 324
MaeI CTAG 3 cut(s) 47, 264, 947
MaeII ACGT 2 cut(s) 585, 802
MaeIII GTNAC 6 cut(s) 153, 586, 745, 942, 1043, 1218
MalI GATC 5 cut(s) 141, 389, 464, 908, 1059
MboI GATC 5 cut(s) 139, 387, 462, 906, 1057
MboII GAAGA 2 cut(s) 600, 971
MhlI GDGCHC 1 cut(s) 410
MluCI AATT 8 cut(s) 92, 124, 176, 272, 871, 881, 1156, 1275
MlyI GAGTC 3 cut(s) 524, 801, 1238
MmeI TCCRAC 1 cut(s) 884
Mph1103I ATGCAT 1 cut(s) 1271
MroNI GCCGGC 1 cut(s) 621
MseI TTAA 2 cut(s) 555, 1073
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 7 cut(s) 186, 202, 219, 223, 411, 612, 622
MspR9I CCNGG 3 cut(s) 224, 326, 613
Mva1269I GAATGC 3 cut(s) 306, 702, 1271
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 2 cut(s) 245, 861
MwoI GCNNNNNNNGC 7 cut(s) 219, 303, 557, 650, 858, 1145, 1172
NaeI GCCGGC 1 cut(s) 623
NciI CCSGG 2 cut(s) 224, 613
NdeI CATATG 1 cut(s) 646
NdeII GATC 5 cut(s) 139, 387, 462, 906, 1057
NgoMIV GCCGGC 1 cut(s) 621
NlaIII CATG 5 cut(s) 469, 799, 881, 891, 986
NlaIV GGNNCC 4 cut(s) 132, 323, 617, 781
NmuCI GTSAC 3 cut(s) 942, 1043, 1218
NsbI TGCGCA 1 cut(s) 1281
NsiI ATGCAT 1 cut(s) 1271
NspV TTCGAA 1 cut(s) 677
PagI TCATGA 3 cut(s) 465, 877, 982
PcsI WCGNNNNNNNCGW 1 cut(s) 808
PctI GAATGC 3 cut(s) 306, 702, 1271
PdiI GCCGGC 1 cut(s) 623
PfeI GAWTC 4 cut(s) 112, 457, 515, 926
PleI GAGTC 3 cut(s) 524, 800, 1237
PpsI GAGTC 3 cut(s) 524, 800, 1237
Ppu21I YACGTR 1 cut(s) 803
PshBI ATTAAT 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 4 cut(s) 132, 323, 617, 781
PspPI GGNCC 5 cut(s) 159, 616, 779, 959, 974
PstI CTGCAG 1 cut(s) 923
PstNI CAGNNNCTG 2 cut(s) 855, 1055
RsaI GTAC 3 cut(s) 254, 282, 323
RsaNI GTAC 3 cut(s) 253, 281, 322
Rsr2I CGGWCCG 1 cut(s) 159
RsrII CGGWCCG 1 cut(s) 159
SacI GAGCTC 1 cut(s) 410
SaqAI TTAA 2 cut(s) 555, 1073
Sau3AI GATC 5 cut(s) 139, 387, 462, 906, 1057
Sau96I GGNCC 5 cut(s) 159, 616, 779, 959, 974
SchI GAGTC 3 cut(s) 524, 801, 1238
ScrFI CCNGG 3 cut(s) 224, 326, 613
SduI GDGCHC 1 cut(s) 410
SexAI ACCWGGT 1 cut(s) 324
SfaNI GCATC 5 cut(s) 277, 432, 442, 657, 976
SfcI CTRYAG 2 cut(s) 919, 1289
SfuI TTCGAA 1 cut(s) 677
SinI GGWCC 3 cut(s) 159, 779, 959
SmlI CTYRAG 2 cut(s) 400, 1241
SmoI CTYRAG 2 cut(s) 400, 1241
SpeI ACTAGT 1 cut(s) 946
Sse9I AATT 8 cut(s) 92, 124, 176, 272, 871, 881, 1156, 1275
SspMI CTAG 3 cut(s) 47, 264, 947
SstI GAGCTC 1 cut(s) 410
StyD4I CCNGG 3 cut(s) 222, 324, 611
TaaI ACNGT 4 cut(s) 842, 866, 1110, 1253
TaiI ACGT 2 cut(s) 588, 805
TaqI TCGA 4 cut(s) 142, 180, 677, 1227
TaqII GACCGA 3 cut(s) 176, 300, 485
TasI AATT 8 cut(s) 92, 124, 176, 272, 871, 881, 1156, 1275
TatI WGTACW 2 cut(s) 252, 280
TauI GCSGC 7 cut(s) 245, 300, 306, 621, 628, 644, 861
TfiI GAWTC 4 cut(s) 112, 457, 515, 926
Tru1I TTAA 2 cut(s) 555, 1073
Tru9I TTAA 2 cut(s) 555, 1073
TscAI CASTG 2 cut(s) 928, 1048
TseFI GTSAC 3 cut(s) 942, 1043, 1218
TseI GCWGC 3 cut(s) 300, 525, 989
Tsp45I GTSAC 3 cut(s) 942, 1043, 1218
TspDTI ATGAA 6 cut(s) 750, 876, 894, 909, 971, 1108
TspGWI ACGGA 3 cut(s) 172, 407, 792
TspRI CASTG 2 cut(s) 928, 1048
VpaK11BI GGWCC 3 cut(s) 159, 779, 959
VspI ATTAAT 1 cut(s) 555
XagI CCTNNNNNAGG 2 cut(s) 329, 1193
XapI RAATTY 5 cut(s) 92, 176, 871, 881, 1156
XbaI TCTAGA 1 cut(s) 263
XspI CTAG 3 cut(s) 47, 264, 947
Zsp2I ATGCAT 1 cut(s) 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.