FvH4_7g25740

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009588
Physical Location & Seq
Reverse (-)
19636450 .. 19639233
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25740.t3

Sequence Viewer

Length: 1281 bp
ATGTCGTTGGCTCTCTCTTCTCCTAAAACCCTATCCCCTTTCTCATTTCTCTGCAACCCTAAACCAAACCCCCAAAACCAAATCCCCAAATTCGCAACCCTAAACCACAACCCGGACCCAATCGAACACAAATCGGAGCACCAAACCGAAAGGGGGCTCAAATTCGACACCGGCGACACCTTCTTCCGGCACGAGAGCGCCACCGGCCGGGACCTCGGGGTCCTCGCGGCGACGCTGTACAAGAAATCCAGAGGCAAACTGCGGGTGCTGGATGCTCTCTGCGGCTGCGGCGTTCGGTCTCTGCGGTACCTGGTGGAGGCTGAAGCTGATTTTGTGTTGGCGAATGACGGAAATGTAGATAGCCGGGACGTGATCACGGCGAACTTGTCTCAGGTGGAGGAGGAGGAGGAGAGGTGGAAGGTGACGCTTACGGATGCGAATCGGATAATGACCGAGTGTTATATGAATAGGGATTTCTATGATCTGATTGACATTGATTCGTTTGGTAGCGACTCGTCGTTTCTCAGGTCGGCGATCAATGCGTTGAGGATGGATGGGTTGCTGTATGTTACCTCCACCGATGGTTTCTCATCTGGTGGGCGCAGGCCGCAGCAAACTTTAGCTGCGTATGGAGCGTATGTTCGGCCTATGCCGTATTCGAATGAGCTTGGTTTGAGGATGCTTATAGGAGGGGTGGTGCGGGTGGCTTCTGTGATGGGGTACCGTGTTGTTCCACTGTTTTCATACTATTCGTATCATGGACCTGTTTTCAGAGTGATGCTGCGTGTCATTCGTGGGAAGCTCCCTGATAGCAGGCATTACGGTTTTATTAGCTACTGCCGCGATTGTGGAAATTCTCAAGAGTTTTCATGGGAAGAACTTGGTCGGATATGTTGCCCCTGCAGTGATTCAAAGGTTTCTGTGTCACTAGTTGTATCTGGTCCTCTCTGGACAGGGCCTCTTCATGATGCTGCTTACATTACAGACATGTTAACTCTAGCAAAGGAATGGGGATGGGCAAGCAGTGACACAGAAACTGATCTTGACAAACTTTTGAATACAATGGTGGCTGAGAGTGACCCTAGATTACCATTTGGCTTCATCAACTTGGACGAGGTAGCAAGCCGTGCAAAAGTAAATTCACCGGCGCTCAAAGCCGTTGTGTCTGCTCTTCAAAAGGAAGGATATGCTGTGAGTAGGTCACATATCGAGTCCAATGCGCTCAAGACAAACTGTCCAATGTCAGAATGCATTAGAATTGCCCAAGAACTACAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

427

Amino Acids

47.43

Weight (kDa)

5.97

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 59 - 365 2.7e-50 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
Acc65I GGTACC 2 cut(s) 306, 720
AccB1I GGYRCC 2 cut(s) 306, 720
AccII CGCG 2 cut(s) 227, 843
AciI CCGC 8 cut(s) 227, 262, 282, 288, 304, 608, 700, 841
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 4 cut(s) 89, 161, 853, 1138
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 3 cut(s) 239, 308, 722
AfiI CCNNNNNNNGG 5 cut(s) 112, 153, 186, 207, 316
AflIII ACRYGT 1 cut(s) 987
AgsI TTSAA 3 cut(s) 912, 1057, 1175
AhdI GACNNNNNGTC 1 cut(s) 1233
AhlI ACTAGT 1 cut(s) 928
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 1 cut(s) 309
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
AluBI AGCT 5 cut(s) 326, 623, 667, 802, 834
AluI AGCT 5 cut(s) 326, 623, 667, 802, 834
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 2 cut(s) 303, 393
AlwNI CAGNNNCTG 1 cut(s) 1037
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 4 cut(s) 205, 605, 644, 956
ApeKI GCWGC 5 cut(s) 285, 610, 623, 781, 971
ApoI RAATTY 4 cut(s) 89, 161, 853, 1138
Asp718I GGTACC 2 cut(s) 306, 720
AspLEI GCGC 4 cut(s) 200, 603, 1150, 1222
AspS9I GGNCC 6 cut(s) 115, 211, 220, 761, 941, 956
AsuC2I CCSGG 3 cut(s) 113, 209, 365
AsuHPI GGTGA 2 cut(s) 433, 1134
AsuII TTCGAA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 5 cut(s) 115, 211, 220, 761, 941
BanI GGYRCC 2 cut(s) 306, 720
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 1 cut(s) 191
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 5 cut(s) 272, 610, 622, 768, 958
BccI CCATC 5 cut(s) 544, 548, 575, 709, 1008
BceAI ACGGC 4 cut(s) 393, 637, 1110, 1142
BcgI CGANNNNNNTGC 2 cut(s) 1199, 1233
BciT130I CCWGG 1 cut(s) 311
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 3 cut(s) 113, 209, 365
BcoDI GTCTC 2 cut(s) 303, 393
BcuI ACTAGT 1 cut(s) 928
BfaI CTAG 3 cut(s) 929, 998, 1083
BfmI CTRYAG 2 cut(s) 901, 1271
BfoI RGCGCY 2 cut(s) 201, 1151
Bme1390I CCNGG 4 cut(s) 113, 209, 311, 365
Bme18I GGWCC 5 cut(s) 115, 211, 220, 761, 941
BmeRI GACNNNNNGTC 1 cut(s) 1233
BmeT110I CYCGRG 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 6 cut(s) 115, 211, 220, 761, 941, 956
BmiI GGNNCC 5 cut(s) 117, 212, 221, 308, 722
BmrFI CCNGG 4 cut(s) 113, 209, 311, 365
BmsI GCATC 5 cut(s) 262, 424, 669, 768, 958
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 2 cut(s) 843, 1208
BpuMI CCSGG 3 cut(s) 113, 209, 365
BsaBI GATNNNNATC 1 cut(s) 438
BsaI GGTCTC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 214
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 624, 654, 681, 711
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 153, 186, 207, 316
Bse118I RCCGGY 3 cut(s) 170, 203, 1144
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 6 cut(s) 277, 439, 555, 559, 684, 1019
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 5 cut(s) 112, 153, 186, 207, 316
BseMII CTCAG 3 cut(s) 404, 538, 1062
BseRI GAGGAG 4 cut(s) 413, 416, 419, 422
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 5 cut(s) 272, 610, 622, 768, 958
Bsh1236I CGCG 2 cut(s) 227, 843
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 4 cut(s) 207, 607, 646, 958
BshNI GGYRCC 2 cut(s) 306, 720
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 7 cut(s) 113, 171, 187, 204, 208, 364, 1145
BslFI GGGAC 2 cut(s) 224, 380
BslI CCNNNNNNNGG 5 cut(s) 112, 153, 186, 207, 316
BsmAI GTCTC 2 cut(s) 303, 393
BsmFI GGGAC 2 cut(s) 224, 380
BsmI GAATGC 1 cut(s) 1253
BsnI GGCC 4 cut(s) 207, 607, 646, 958
Bso31I GGTCTC 1 cut(s) 303
BsoBI CYCGRG 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 141, 159
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 4 cut(s) 372, 481, 534, 1039
BspACI CCGC 8 cut(s) 227, 262, 282, 288, 304, 608, 700, 841
BspANI GGCC 4 cut(s) 207, 607, 646, 958
BspCNI CTCAG 3 cut(s) 403, 537, 1063
BspFNI CGCG 2 cut(s) 227, 843
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 5 cut(s) 117, 212, 221, 308, 722
BspMAI CTGCAG 1 cut(s) 905
BspQI GCTCTTC 1 cut(s) 1176
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 2 cut(s) 306, 720
BspTNI GGTCTC 1 cut(s) 303
BsrFI RCCGGY 3 cut(s) 170, 203, 1144
BsrGI TGTACA 1 cut(s) 237
BssAI RCCGGY 3 cut(s) 170, 203, 1144
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 4 cut(s) 372, 481, 534, 1039
BssSI CACGAG 1 cut(s) 191
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 4 cut(s) 725, 738, 824, 1235
Bst6I CTCTTC 3 cut(s) 22, 966, 1176
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstAUI TGTACA 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 4 cut(s) 605, 815, 1021, 1123
BstDEI CTNAG 3 cut(s) 390, 524, 1071
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 6 cut(s) 277, 439, 555, 559, 684, 1019
BstFNI CGCG 2 cut(s) 227, 843
BstH2I RGCGCY 2 cut(s) 201, 1151
BstHHI GCGC 4 cut(s) 200, 603, 1150, 1222
BstKTI GATC 4 cut(s) 375, 484, 537, 1042
BstMAI GTCTC 2 cut(s) 303, 393
BstMBI GATC 4 cut(s) 372, 481, 534, 1039
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 8 cut(s) 204, 288, 539, 607, 632, 840, 1127, 1154
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 991
BstSCI CCNGG 4 cut(s) 111, 207, 309, 363
BstSFI CTRYAG 2 cut(s) 901, 1271
BstUI CGCG 2 cut(s) 227, 843
BstV1I GCAGC 5 cut(s) 272, 610, 622, 768, 958
BstZI CGGCCG 1 cut(s) 205
BsuRI GGCC 4 cut(s) 207, 607, 646, 958
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 6 cut(s) 277, 439, 555, 559, 684, 1019
BtsI GCAGTG 2 cut(s) 910, 1030
BtsIMutI CAGTG 3 cut(s) 734, 910, 1030
Cac8I GCNNGC 4 cut(s) 605, 815, 1021, 1123
CaiI CAGNNNCTG 1 cut(s) 1037
CciI TCATGA 1 cut(s) 964
CfoI GCGC 4 cut(s) 200, 603, 1150, 1222
Cfr10I RCCGGY 3 cut(s) 170, 203, 1144
Cfr13I GGNCC 6 cut(s) 115, 211, 220, 761, 941, 956
CseI GACGC 2 cut(s) 241, 433
CsiI ACCWGGT 1 cut(s) 309
Csp6I GTAC 3 cut(s) 238, 307, 721
CviAII CATG 4 cut(s) 758, 870, 965, 988
CviQI GTAC 3 cut(s) 238, 307, 721
DdeI CTNAG 3 cut(s) 390, 524, 1071
DpnI GATC 4 cut(s) 374, 483, 536, 1041
DpnII GATC 4 cut(s) 372, 481, 534, 1039
DrdI GACNNNNNNGTC 1 cut(s) 218
DriI GACNNNNNGTC 1 cut(s) 1233
DseDI GACNNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 1 cut(s) 205
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 3 cut(s) 22, 966, 1176
Eam1105I GACNNNNNGTC 1 cut(s) 1233
EarI CTCTTC 3 cut(s) 22, 966, 1176
EclXI CGGCCG 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 303
Eco47I GGWCC 5 cut(s) 115, 211, 220, 761, 941
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 215
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO109I RGGNCCY 3 cut(s) 211, 220, 956
EcoRII CCWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 1253
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 4 cut(s) 761, 873, 968, 991
FaqI GGGAC 2 cut(s) 224, 380
FatI CATG 4 cut(s) 757, 869, 964, 987
FauI CCCGC 2 cut(s) 255, 693
FbaI TGATCA 1 cut(s) 372
FokI GGATG 6 cut(s) 284, 446, 562, 566, 691, 1026
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 3 cut(s) 929, 998, 1083
GlaI GCGC 4 cut(s) 199, 602, 1149, 1221
HaeII RGCGCY 2 cut(s) 201, 1151
HaeIII GGCC 4 cut(s) 207, 607, 646, 958
HapII CCGG 7 cut(s) 113, 171, 187, 204, 208, 364, 1145
HgaI GACGC 2 cut(s) 241, 433
HhaI GCGC 4 cut(s) 200, 603, 1150, 1222
Hin1II CATG 4 cut(s) 761, 873, 968, 991
Hin6I GCGC 4 cut(s) 198, 601, 1148, 1220
HinP1I GCGC 4 cut(s) 198, 601, 1148, 1220
HincII GTYRAC 1 cut(s) 993
HindII GTYRAC 1 cut(s) 993
HinfI GANTC 5 cut(s) 439, 497, 512, 908, 1211
HpaI GTTAAC 1 cut(s) 993
HpaII CCGG 7 cut(s) 113, 171, 187, 204, 208, 364, 1145
HphI GGTGA 2 cut(s) 433, 1134
Hpy166II GTNNAC 1 cut(s) 993
Hpy188I TCNGA 6 cut(s) 136, 444, 486, 773, 888, 1246
Hpy188III TCNNGA 6 cut(s) 249, 860, 949, 965, 1043, 1225
Hpy8I GTNNAC 1 cut(s) 993
Hpy99I CGWCG 2 cut(s) 235, 520
HpyAV CCTTC 3 cut(s) 190, 412, 1175
HpyCH4III ACNGT 4 cut(s) 725, 738, 824, 1235
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 54, 903, 1130, 1251
HpyF10VI GCNNNNNNNGC 8 cut(s) 204, 288, 539, 607, 632, 840, 1127, 1154
HpyF3I CTNAG 3 cut(s) 390, 524, 1071
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 4 cut(s) 761, 873, 968, 991
HspAI GCGC 4 cut(s) 198, 601, 1148, 1220
KpnI GGTACC 2 cut(s) 310, 724
Ksp22I TGATCA 1 cut(s) 372
KspAI GTTAAC 1 cut(s) 993
Kzo9I GATC 4 cut(s) 372, 481, 534, 1039
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 3 cut(s) 136, 632, 807
Lsp1109I GCAGC 5 cut(s) 272, 610, 622, 768, 958
LweI GCATC 5 cut(s) 262, 424, 669, 768, 958
MabI ACCWGGT 1 cut(s) 309
MaeI CTAG 3 cut(s) 929, 998, 1083
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 6 cut(s) 421, 568, 924, 1025, 1076, 1200
MalI GATC 4 cut(s) 374, 483, 536, 1041
MboI GATC 4 cut(s) 372, 481, 534, 1039
MboII GAAGA 5 cut(s) 9, 175, 887, 953, 1163
MhlI GDGCHC 2 cut(s) 141, 159
MluCI AATT 5 cut(s) 89, 161, 853, 1138, 1257
MlyI GAGTC 2 cut(s) 506, 1220
MmeI TCCRAC 1 cut(s) 866
Mph1103I ATGCAT 1 cut(s) 1253
MseI TTAA 1 cut(s) 992
MspI CCGG 7 cut(s) 113, 171, 187, 204, 208, 364, 1145
MspR9I CCNGG 4 cut(s) 113, 209, 311, 365
Mva1269I GAATGC 1 cut(s) 1253
MvaI CCWGG 1 cut(s) 311
MvnI CGCG 2 cut(s) 227, 843
MwoI GCNNNNNNNGC 8 cut(s) 204, 288, 539, 607, 632, 840, 1127, 1154
NciI CCSGG 3 cut(s) 113, 209, 365
NdeII GATC 4 cut(s) 372, 481, 534, 1039
NlaIII CATG 4 cut(s) 761, 873, 968, 991
NlaIV GGNNCC 5 cut(s) 117, 212, 221, 308, 722
NmuCI GTSAC 5 cut(s) 421, 924, 1025, 1076, 1200
NsiI ATGCAT 1 cut(s) 1253
NspI RCATGY 1 cut(s) 991
NspV TTCGAA 1 cut(s) 659
PagI TCATGA 1 cut(s) 964
PciI ACATGT 1 cut(s) 987
PciSI GCTCTTC 1 cut(s) 1176
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 1 cut(s) 1253
PfeI GAWTC 3 cut(s) 439, 497, 908
PleI GAGTC 2 cut(s) 506, 1219
PpsI GAGTC 2 cut(s) 506, 1219
PpuMI RGGWCCY 2 cut(s) 211, 220
PscI ACATGT 1 cut(s) 987
Psp5II RGGWCCY 2 cut(s) 211, 220
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 5 cut(s) 117, 212, 221, 308, 722
PspPI GGNCC 6 cut(s) 115, 211, 220, 761, 941, 956
PspPPI RGGWCCY 2 cut(s) 211, 220
PstI CTGCAG 1 cut(s) 905
PstNI CAGNNNCTG 1 cut(s) 1037
RsaI GTAC 3 cut(s) 239, 308, 722
RsaNI GTAC 3 cut(s) 238, 307, 721
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 1 cut(s) 992
Sau3AI GATC 4 cut(s) 372, 481, 534, 1039
Sau96I GGNCC 6 cut(s) 115, 211, 220, 761, 941, 956
SchI GAGTC 2 cut(s) 506, 1220
ScrFI CCNGG 4 cut(s) 113, 209, 311, 365
SduI GDGCHC 2 cut(s) 141, 159
SexAI ACCWGGT 1 cut(s) 309
SfaNI GCATC 5 cut(s) 262, 424, 669, 768, 958
SfcI CTRYAG 2 cut(s) 901, 1271
SfuI TTCGAA 1 cut(s) 659
SgrAI CRCCGGYG 2 cut(s) 170, 1144
SinI GGWCC 5 cut(s) 115, 211, 220, 761, 941
SmlI CTYRAG 2 cut(s) 858, 1223
SmoI CTYRAG 2 cut(s) 858, 1223
SpeI ACTAGT 1 cut(s) 928
Sse9I AATT 5 cut(s) 89, 161, 853, 1138, 1257
SsiI CCGC 8 cut(s) 227, 262, 282, 288, 304, 608, 700, 841
SspMI CTAG 3 cut(s) 929, 998, 1083
StyD4I CCNGG 4 cut(s) 111, 207, 309, 363
TaaI ACNGT 4 cut(s) 725, 738, 824, 1235
TaiI ACGT 1 cut(s) 372
TaqI TCGA 4 cut(s) 123, 165, 659, 1209
TaqII GACCGA 2 cut(s) 285, 467
TasI AATT 5 cut(s) 89, 161, 853, 1138, 1257
TatI WGTACW 1 cut(s) 237
TauI GCSGC 5 cut(s) 230, 285, 291, 610, 843
TfiI GAWTC 3 cut(s) 439, 497, 908
Tru1I TTAA 1 cut(s) 992
Tru9I TTAA 1 cut(s) 992
TscAI CASTG 3 cut(s) 741, 910, 1030
TseFI GTSAC 5 cut(s) 421, 924, 1025, 1076, 1200
TseI GCWGC 5 cut(s) 285, 610, 623, 781, 971
Tsp45I GTSAC 5 cut(s) 421, 924, 1025, 1076, 1200
TspDTI ATGAA 5 cut(s) 479, 732, 858, 953, 1090
TspGWI ACGGA 2 cut(s) 363, 446
TspRI CASTG 3 cut(s) 741, 910, 1030
VpaK11BI GGWCC 5 cut(s) 115, 211, 220, 761, 941
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 4 cut(s) 89, 161, 853, 1138
XceI RCATGY 1 cut(s) 991
XspI CTAG 3 cut(s) 929, 998, 1083
Zsp2I ATGCAT 1 cut(s) 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.