Rroxscaffold_4G00285330

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009588
Physical Location & Seq
Forward (+)
6682270 .. 6687039
4770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285330.1

Sequence Viewer

Length: 1299 bp
ATGTTGTTGGCTCTCGCTCCTAAAACCCTATCCCCTTTCTCATTTCTCCCCAACCCAAAACCAAGCCCCAAAAACCAAATCCCCAAATTCGCAACCCTAAACCAGAATCCAGTCCCCAATTCGGAGCCGATTGATCGAAAATGCGAGTATCGAACCGAGAGAGGTCTCAAATTCGACACCGGAGAGACATTTTTCCGGCACGAGAGCGCCACCGGCCGGGATTTGGGTGTTCTGGCCGCGACGCTGTACAAGAAATCTAGAGGGAAATTGCGTGTACTCGATGCCCTCTGCGGCTGCGGGATTCGGTCTCTGCGGTACCTGGTGGAGGCGGAGGCGGATTTTGTGTTGGCGAATGATGGAAATGCTGAGAGCAGGGATGTGATTACGGAGAACTTGAGGAGCTCCGGCGAGGAGGAGGAGGAGGAGGAGAGGTGGAAGGTGACGCTTATGGATGCGAATCGGATCATGACCGAGTGTTATCTGAACAGGGATTTCTATGACCTCATTGACATTGATTCGTTTGGCAGCGACTCGTCGTTTCTCAGGTCGGCGATTAATGCTTTGAGGATGGATGGCTTGCTGTATGTTACCTCCACCGATGGTTTCTCTTCCGGTGGTCGCCGGCCGCAACAAACTTTAGCTGCATATGGAGCTTATGTTCGGCCTATGCCATATTCGAATGAGCTTGGTTTGAGAATGCTTATAGGAGGGGTTGTGCGGGTAGCTTCTGTGATGGGGTATCGTGTTACTCCGCTGTTTTCATACTATTCGTATCATGGACCTGTTTTCAGGGTCATGCTACGTGTCATTCGTGGAAAGCTCCCTGATAGCAGACACTACGGTTTTATCAGCTACTGCCGCGACTGTGGAAATTCTCATGAATTTTCATGGGATGAACTTGGTCGGATCAGTTGCCCCTGCAGTGATTCAAAGGTTTCTATGTCACTAGTTGTTTCTGGTCCTCTCTGGACAGGGCCTCTTCATGATGCTGCTTACATTACAGACATGTTAACTTTAGCAAAGGAATGGGGATGGGCGGGCAGTGACACAGGAACTGATCTTGACAAACTTTTAAATACAATGGTGGCTGAGAGCGACCCCAGATTACCGTTTGGCTTCATCAACTTGGACGAGGTAGCAAGCCGTGCAAAAGTAAATTCACCAGCACTCAAAGCTGTGGTGTCTGCCCTTCAAAAGGAGGGATATGCTGTGAGTAGGTCACATATCGAGTCCAATGCACTCAAGACCAACTGTCCAATGTCAGAATGCATTAGAATTGCGCAAGAACTACAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

432

Amino Acids

48.12

Weight (kDa)

6.15

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 61 - 426 1e-64 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1281
Acc65I GGTACC 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 315
AccII CGCG 2 cut(s) 239, 861
AclWI GGATC 2 cut(s) 470, 914
AcoI YGGCCR 3 cut(s) 214, 234, 623
AcsI RAATTY 5 cut(s) 86, 170, 871, 881, 1156
AfaI GTAC 3 cut(s) 248, 276, 317
AfiI CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1195
AflIII ACRYGT 2 cut(s) 802, 1005
AgsI TTSAA 2 cut(s) 930, 1193
AhdI GACNNNNNGTC 1 cut(s) 1251
AhlI ACTAGT 1 cut(s) 946
AjnI CCWGG 1 cut(s) 318
AloI GAACNNNNNNTCC 2 cut(s) 642, 674
AluBI AGCT 8 cut(s) 402, 641, 653, 685, 725, 820, 852, 1175
AluI AGCT 8 cut(s) 402, 641, 653, 685, 725, 820, 852, 1175
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 3 cut(s) 170, 179, 312
AlwI GGATC 2 cut(s) 470, 914
AlwNI CAGNNNCTG 2 cut(s) 855, 1055
AoxI GGCC 5 cut(s) 214, 234, 623, 662, 974
ApeKI GCWGC 4 cut(s) 294, 525, 641, 989
ApoI RAATTY 5 cut(s) 86, 170, 871, 881, 1156
AseI ATTAAT 1 cut(s) 555
Asp718I GGTACC 1 cut(s) 315
AspLEI GCGC 2 cut(s) 209, 1282
AspS9I GGNCC 3 cut(s) 779, 959, 974
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 451, 1152
AsuII TTCGAA 1 cut(s) 677
AvaII GGWCC 2 cut(s) 779, 959
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 404
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 4 cut(s) 281, 537, 628, 976
BccI CCATC 6 cut(s) 350, 562, 566, 593, 727, 1026
BceAI ACGGC 1 cut(s) 1128
BcgI CGANNNNNNTGC 2 cut(s) 1217, 1251
BciT130I CCWGG 1 cut(s) 320
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 3 cut(s) 170, 179, 312
BcuI ACTAGT 1 cut(s) 946
BfaI CTAG 2 cut(s) 258, 947
BfmI CTRYAG 2 cut(s) 919, 1289
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 8 cut(s) 237, 292, 295, 526, 626, 642, 859, 990
BlsI GCNGC 8 cut(s) 238, 293, 296, 527, 627, 643, 860, 991
Bme1390I CCNGG 2 cut(s) 218, 320
Bme18I GGWCC 2 cut(s) 779, 959
BmeRI GACNNNNNGTC 1 cut(s) 1251
BmgT120I GGNCC 3 cut(s) 779, 959, 974
BmiI GGNNCC 2 cut(s) 126, 317
BmrFI CCNGG 2 cut(s) 218, 320
BmsI GCATC 3 cut(s) 271, 442, 976
Bpu14I TTCGAA 1 cut(s) 677
BpuEI CTTGAG 2 cut(s) 415, 1226
BpuMI CCSGG 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 803
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 2 cut(s) 170, 312
BsaWI WCCGGW 2 cut(s) 179, 611
BsaXI ACNNNNNCTCC 6 cut(s) 416, 446, 642, 672, 699, 729
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1195
Bse118I RCCGGY 2 cut(s) 212, 621
Bse1I ACTGG 1 cut(s) 110
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 1 cut(s) 320
BseGI GGATG 6 cut(s) 382, 457, 573, 577, 898, 1037
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1195
BseMII CTCAG 3 cut(s) 357, 556, 1080
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 7 cut(s) 412, 425, 428, 431, 434, 437, 440
BseX3I CGGCCG 2 cut(s) 214, 623
BseXI GCAGC 4 cut(s) 281, 537, 628, 976
Bsh1236I CGCG 2 cut(s) 239, 861
Bsh1285I CGRYCG 2 cut(s) 217, 626
BshFI GGCC 5 cut(s) 216, 236, 625, 664, 976
BshNI GGYRCC 1 cut(s) 315
BsiEI CGRYCG 2 cut(s) 217, 626
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 7 cut(s) 180, 196, 213, 217, 405, 612, 622
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 5 cut(s) 121, 216, 223, 325, 1195
BsmAI GTCTC 3 cut(s) 170, 179, 312
BsmFI GGGAC 1 cut(s) 98
BsmI GAATGC 2 cut(s) 702, 1271
BsnI GGCC 5 cut(s) 216, 236, 625, 664, 976
Bso31I GGTCTC 2 cut(s) 170, 312
Bsp119I TTCGAA 1 cut(s) 677
Bsp1286I GDGCHC 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 4 cut(s) 133, 462, 906, 1057
BspANI GGCC 5 cut(s) 216, 236, 625, 664, 976
BspCNI CTCAG 3 cut(s) 358, 555, 1081
BspFNI CGCG 2 cut(s) 239, 861
BspHI TCATGA 3 cut(s) 465, 877, 982
BspLI GGNNCC 2 cut(s) 126, 317
BspMAI CTGCAG 1 cut(s) 923
BspPI GGATC 2 cut(s) 470, 914
BspT104I TTCGAA 1 cut(s) 677
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 2 cut(s) 170, 312
BsrFI RCCGGY 2 cut(s) 212, 621
BsrGI TGTACA 1 cut(s) 246
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 2 cut(s) 212, 621
BssMI GATC 4 cut(s) 133, 462, 906, 1057
BssSI CACGAG 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 4 cut(s) 842, 866, 1110, 1253
Bst6I CTCTTC 2 cut(s) 613, 984
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstAUI TGTACA 1 cut(s) 246
BstBAI YACGTR 1 cut(s) 803
BstBI TTCGAA 1 cut(s) 677
BstC8I GCNNGC 4 cut(s) 578, 623, 1039, 1141
BstDEI CTNAG 3 cut(s) 366, 542, 1089
BstENI CCTNNNNNAGG 2 cut(s) 323, 1193
BstF5I GGATG 6 cut(s) 382, 457, 573, 577, 898, 1037
BstFNI CGCG 2 cut(s) 239, 861
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 2 cut(s) 209, 1282
BstKTI GATC 4 cut(s) 136, 465, 909, 1060
BstMAI GTCTC 3 cut(s) 170, 179, 312
BstMBI GATC 4 cut(s) 133, 462, 906, 1057
BstMCI CGRYCG 2 cut(s) 217, 626
BstMWI GCNNNNNNNGC 6 cut(s) 213, 557, 650, 858, 1145, 1172
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 1009
BstSCI CCNGG 2 cut(s) 216, 318
BstSFI CTRYAG 2 cut(s) 919, 1289
BstUI CGCG 2 cut(s) 239, 861
BstV1I GCAGC 4 cut(s) 281, 537, 628, 976
BstZI CGGCCG 2 cut(s) 214, 623
BsuRI GGCC 5 cut(s) 216, 236, 625, 664, 976
BtsCI GGATG 6 cut(s) 382, 457, 573, 577, 898, 1037
BtsI GCAGTG 2 cut(s) 928, 1048
BtsIMutI CAGTG 2 cut(s) 928, 1048
Cac8I GCNNGC 4 cut(s) 578, 623, 1039, 1141
CaiI CAGNNNCTG 2 cut(s) 855, 1055
CciI TCATGA 3 cut(s) 465, 877, 982
CfoI GCGC 2 cut(s) 209, 1282
Cfr10I RCCGGY 2 cut(s) 212, 621
Cfr13I GGNCC 3 cut(s) 779, 959, 974
CseI GACGC 2 cut(s) 250, 451
CsiI ACCWGGT 1 cut(s) 318
Csp6I GTAC 3 cut(s) 247, 275, 316
CviAII CATG 7 cut(s) 466, 776, 796, 878, 888, 983, 1006
CviQI GTAC 3 cut(s) 247, 275, 316
DdeI CTNAG 3 cut(s) 366, 542, 1089
DpnI GATC 4 cut(s) 135, 464, 908, 1059
DpnII GATC 4 cut(s) 133, 462, 906, 1057
DraI TTTAAA 1 cut(s) 1074
DriI GACNNNNNGTC 1 cut(s) 1251
EaeI YGGCCR 3 cut(s) 214, 234, 623
EagI CGGCCG 2 cut(s) 214, 623
Eam1104I CTCTTC 2 cut(s) 613, 984
Eam1105I GACNNNNNGTC 1 cut(s) 1251
EarI CTCTTC 2 cut(s) 613, 984
EciI GGCGGA 2 cut(s) 344, 350
Ecl136II GAGCTC 1 cut(s) 402
EclXI CGGCCG 2 cut(s) 214, 623
Eco24I GRGCYC 1 cut(s) 404
Eco31I GGTCTC 2 cut(s) 170, 312
Eco47I GGWCC 2 cut(s) 779, 959
Eco52I CGGCCG 2 cut(s) 214, 623
Eco53kI GAGCTC 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 402
EcoNI CCTNNNNNAGG 2 cut(s) 323, 1193
EcoO109I RGGNCCY 1 cut(s) 974
EcoRII CCWGG 1 cut(s) 318
EcoT22I ATGCAT 1 cut(s) 1271
EcoT38I GRGCYC 1 cut(s) 404
FaeI CATG 7 cut(s) 469, 779, 799, 881, 891, 986, 1009
FaqI GGGAC 1 cut(s) 98
FatI CATG 7 cut(s) 465, 775, 795, 877, 887, 982, 1005
FauI CCCGC 3 cut(s) 290, 711, 1030
FauNDI CATATG 1 cut(s) 646
Fnu4HI GCNGC 8 cut(s) 237, 292, 295, 526, 626, 642, 859, 990
FokI GGATG 6 cut(s) 389, 464, 580, 584, 905, 1044
FriOI GRGCYC 1 cut(s) 404
Fsp4HI GCNGC 8 cut(s) 237, 292, 295, 526, 626, 642, 859, 990
FspBI CTAG 2 cut(s) 258, 947
FspI TGCGCA 1 cut(s) 1281
GlaI GCGC 2 cut(s) 208, 1281
GluI GCNGC 8 cut(s) 237, 292, 295, 526, 626, 642, 859, 990
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 5 cut(s) 216, 236, 625, 664, 976
HapII CCGG 7 cut(s) 180, 196, 213, 217, 405, 612, 622
HgaI GACGC 2 cut(s) 250, 451
HhaI GCGC 2 cut(s) 209, 1282
Hin1II CATG 7 cut(s) 469, 779, 799, 881, 891, 986, 1009
Hin6I GCGC 2 cut(s) 207, 1280
HinP1I GCGC 2 cut(s) 207, 1280
HincII GTYRAC 1 cut(s) 1011
HindII GTYRAC 1 cut(s) 1011
HinfI GANTC 7 cut(s) 106, 301, 457, 515, 530, 926, 1229
HpaI GTTAAC 1 cut(s) 1011
HpaII CCGG 7 cut(s) 180, 196, 213, 217, 405, 612, 622
HphI GGTGA 2 cut(s) 451, 1152
Hpy166II GTNNAC 2 cut(s) 275, 1011
Hpy188I TCNGA 5 cut(s) 124, 462, 483, 906, 1264
Hpy188III TCNNGA 7 cut(s) 258, 466, 878, 967, 983, 1061, 1243
Hpy8I GTNNAC 2 cut(s) 275, 1011
Hpy99I CGWCG 2 cut(s) 244, 538
HpyAV CCTTC 2 cut(s) 430, 1199
HpyCH4III ACNGT 4 cut(s) 842, 866, 1110, 1253
HpyCH4IV ACGT 1 cut(s) 802
HpyCH4V TGCA 5 cut(s) 644, 921, 1148, 1238, 1269
HpyF10VI GCNNNNNNNGC 6 cut(s) 213, 557, 650, 858, 1145, 1172
HpyF3I CTNAG 3 cut(s) 366, 542, 1089
HpySE526I ACGT 1 cut(s) 802
Hsp92II CATG 7 cut(s) 469, 779, 799, 881, 891, 986, 1009
HspAI GCGC 2 cut(s) 207, 1280
KpnI GGTACC 1 cut(s) 319
KroI GCCGGC 1 cut(s) 621
KroNI GCCGGC 1 cut(s) 623
KspAI GTTAAC 1 cut(s) 1011
Kzo9I GATC 4 cut(s) 133, 462, 906, 1057
LmnI GCTCC 6 cut(s) 22, 124, 399, 407, 650, 825
Lsp1109I GCAGC 4 cut(s) 281, 537, 628, 976
LweI GCATC 3 cut(s) 271, 442, 976
MabI ACCWGGT 1 cut(s) 318
MaeI CTAG 2 cut(s) 258, 947
MaeII ACGT 1 cut(s) 802
MaeIII GTNAC 6 cut(s) 439, 586, 745, 942, 1043, 1218
MalI GATC 4 cut(s) 135, 464, 908, 1059
MboI GATC 4 cut(s) 133, 462, 906, 1057
MboII GAAGA 2 cut(s) 600, 971
MhlI GDGCHC 1 cut(s) 404
MluCI AATT 8 cut(s) 86, 118, 170, 266, 871, 881, 1156, 1275
MlyI GAGTC 2 cut(s) 524, 1238
MmeI TCCRAC 1 cut(s) 884
Mph1103I ATGCAT 1 cut(s) 1271
MroNI GCCGGC 1 cut(s) 621
MseI TTAA 3 cut(s) 555, 1010, 1073
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 7 cut(s) 180, 196, 213, 217, 405, 612, 622
MspR9I CCNGG 2 cut(s) 218, 320
Mva1269I GAATGC 2 cut(s) 702, 1271
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 2 cut(s) 239, 861
MwoI GCNNNNNNNGC 6 cut(s) 213, 557, 650, 858, 1145, 1172
NaeI GCCGGC 1 cut(s) 623
NciI CCSGG 1 cut(s) 218
NdeI CATATG 1 cut(s) 646
NdeII GATC 4 cut(s) 133, 462, 906, 1057
NgoMIV GCCGGC 1 cut(s) 621
NlaIII CATG 7 cut(s) 469, 779, 799, 881, 891, 986, 1009
NlaIV GGNNCC 2 cut(s) 126, 317
NmuCI GTSAC 4 cut(s) 439, 942, 1043, 1218
NsbI TGCGCA 1 cut(s) 1281
NsiI ATGCAT 1 cut(s) 1271
NspI RCATGY 1 cut(s) 1009
NspV TTCGAA 1 cut(s) 677
PagI TCATGA 3 cut(s) 465, 877, 982
PciI ACATGT 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 808
PctI GAATGC 2 cut(s) 702, 1271
PdiI GCCGGC 1 cut(s) 623
PfeI GAWTC 5 cut(s) 106, 301, 457, 515, 926
PkrI GCNGC 8 cut(s) 238, 293, 296, 527, 627, 643, 860, 991
PleI GAGTC 2 cut(s) 524, 1237
PpsI GAGTC 2 cut(s) 524, 1237
Ppu21I YACGTR 1 cut(s) 803
PscI ACATGT 1 cut(s) 1005
PshBI ATTAAT 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 126, 317
PspPI GGNCC 3 cut(s) 779, 959, 974
PstI CTGCAG 1 cut(s) 923
PstNI CAGNNNCTG 2 cut(s) 855, 1055
RsaI GTAC 3 cut(s) 248, 276, 317
RsaNI GTAC 3 cut(s) 247, 275, 316
SacI GAGCTC 1 cut(s) 404
SaqAI TTAA 3 cut(s) 555, 1010, 1073
SatI GCNGC 8 cut(s) 237, 292, 295, 526, 626, 642, 859, 990
Sau3AI GATC 4 cut(s) 133, 462, 906, 1057
Sau96I GGNCC 3 cut(s) 779, 959, 974
SchI GAGTC 2 cut(s) 524, 1238
ScrFI CCNGG 2 cut(s) 218, 320
SduI GDGCHC 1 cut(s) 404
SexAI ACCWGGT 1 cut(s) 318
SfaNI GCATC 3 cut(s) 271, 442, 976
SfcI CTRYAG 2 cut(s) 919, 1289
SfuI TTCGAA 1 cut(s) 677
SinI GGWCC 2 cut(s) 779, 959
SmlI CTYRAG 2 cut(s) 394, 1241
SmoI CTYRAG 2 cut(s) 394, 1241
SpeI ACTAGT 1 cut(s) 946
Sse9I AATT 8 cut(s) 86, 118, 170, 266, 871, 881, 1156, 1275
SspMI CTAG 2 cut(s) 258, 947
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 216, 318
TaaI ACNGT 4 cut(s) 842, 866, 1110, 1253
TaiI ACGT 1 cut(s) 805
TaqI TCGA 6 cut(s) 136, 151, 174, 279, 677, 1227
TaqII GACCGA 2 cut(s) 294, 485
TasI AATT 8 cut(s) 86, 118, 170, 266, 871, 881, 1156, 1275
TatI WGTACW 2 cut(s) 246, 274
TauI GCSGC 4 cut(s) 239, 294, 628, 861
TfiI GAWTC 5 cut(s) 106, 301, 457, 515, 926
Tru1I TTAA 3 cut(s) 555, 1010, 1073
Tru9I TTAA 3 cut(s) 555, 1010, 1073
TscAI CASTG 2 cut(s) 928, 1048
TseFI GTSAC 4 cut(s) 439, 942, 1043, 1218
TseI GCWGC 4 cut(s) 294, 525, 641, 989
Tsp45I GTSAC 4 cut(s) 439, 942, 1043, 1218
TspDTI ATGAA 6 cut(s) 750, 876, 894, 909, 971, 1108
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 928, 1048
VpaK11BI GGWCC 2 cut(s) 779, 959
VspI ATTAAT 1 cut(s) 555
XagI CCTNNNNNAGG 2 cut(s) 323, 1193
XapI RAATTY 5 cut(s) 86, 170, 871, 881, 1156
XbaI TCTAGA 1 cut(s) 257
XceI RCATGY 1 cut(s) 1009
XspI CTAG 2 cut(s) 258, 947
Zsp2I ATGCAT 1 cut(s) 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.