May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Acts redundantly with BOP2. BOP1 2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1 2 act as transcriptional co- regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14 PHB, YAB1 FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6 AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G41370 AT3G57130 |
| fragaria_vesca | FvH4_6g25090 |
| malus_domestica | MD12G1091100.v1.1 MD14G1083700.v1.1 |
| prunus_persica | Prupe.7G046800_v2.0.a1 |
| pyrus_communis | pycom12g07650 pycom14g07290 |
| rosa_chinensis | RchiOBHm_Chr2g0124891 |
| rosa_laevigata | RLG00000018831 |
| rosa_multiflora | Rmu_sc0005075.1_g000013 |
| rosa_roxburghii | Rroxscaffold_2G00119550 |
| rosa_rugosa | Rorug02G0253100 |
| rosa_samantha | Rh2AG312800 Rh2BG321000 Rh2CG302300 Rh2DG338400 |
| rosa_wichuraiana | Rw0G021780 Rw2G025260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1016 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1027 |
| AccBSI | CCGCTC | 3 cut(s) | 431, 944, 1248 |
| AccII | CGCG | 5 cut(s) | 458, 851, 923, 1157, 1176 |
| AccIII | TCCGGA | 1 cut(s) | 973 |
| AclWI | GGATC | 2 cut(s) | 173, 226 |
| AcsI | RAATTY | 1 cut(s) | 161 |
| AcyI | GRCGYC | 1 cut(s) | 444 |
| AfaI | GTAC | 2 cut(s) | 308, 330 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1027, 1156 |
| AgeI | ACCGGT | 1 cut(s) | 256 |
| AgsI | TTSAA | 7 cut(s) | 299, 509, 537, 675, 857, 1084, 1266 |
| AjiI | CACGTC | 1 cut(s) | 761 |
| AjnI | CCWGG | 1 cut(s) | 1096 |
| Alw21I | GWGCWC | 2 cut(s) | 528, 660 |
| Alw26I | GTCTC | 5 cut(s) | 325, 668, 751, 975, 1142 |
| AlwI | GGATC | 2 cut(s) | 173, 226 |
| Ama87I | CYCGRG | 1 cut(s) | 1139 |
| Aor13HI | TCCGGA | 1 cut(s) | 973 |
| AoxI | GGCC | 1 cut(s) | 103 |
| ApeKI | GCWGC | 5 cut(s) | 137, 233, 395, 451, 902 |
| ApoI | RAATTY | 1 cut(s) | 161 |
| AsiGI | ACCGGT | 1 cut(s) | 256 |
| AspA2I | CCTAGG | 1 cut(s) | 1223 |
| AspS9I | GGNCC | 2 cut(s) | 926, 1147 |
| AsuC2I | CCSGG | 1 cut(s) | 192 |
| AsuHPI | GGTGA | 4 cut(s) | 113, 692, 814, 1376 |
| AsuII | TTCGAA | 2 cut(s) | 15, 1057 |
| AvaI | CYCGRG | 1 cut(s) | 1139 |
| AvaII | GGWCC | 2 cut(s) | 926, 1147 |
| AvrII | CCTAGG | 1 cut(s) | 1223 |
| BanII | GRGCYC | 2 cut(s) | 660, 1119 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 270, 302, 870, 902 |
| BauI | CACGAG | 2 cut(s) | 341, 1243 |
| BbsI | GAAGAC | 2 cut(s) | 590, 741 |
| Bbv12I | GWGCWC | 2 cut(s) | 528, 660 |
| BbvI | GCAGC | 5 cut(s) | 124, 245, 407, 463, 914 |
| BccI | CCATC | 3 cut(s) | 799, 1025, 1375 |
| BceAI | ACGGC | 2 cut(s) | 90, 383 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 29 |
| BciT130I | CCWGG | 1 cut(s) | 1098 |
| BcnI | CCSGG | 1 cut(s) | 192 |
| BcoDI | GTCTC | 5 cut(s) | 325, 668, 751, 975, 1142 |
| BfaI | CTAG | 6 cut(s) | 110, 614, 824, 836, 1224, 1402 |
| BfmI | CTRYAG | 1 cut(s) | 393 |
| BlnI | CCTAGG | 1 cut(s) | 1223 |
| Bme1390I | CCNGG | 2 cut(s) | 192, 1098 |
| Bme18I | GGWCC | 2 cut(s) | 926, 1147 |
| BmeT110I | CYCGRG | 1 cut(s) | 1139 |
| BmgBI | CACGTC | 1 cut(s) | 761 |
| BmgT120I | GGNCC | 2 cut(s) | 926, 1147 |
| BmiI | GGNNCC | 3 cut(s) | 226, 927, 928 |
| BmrFI | CCNGG | 2 cut(s) | 192, 1098 |
| BmsI | GCATC | 4 cut(s) | 138, 362, 562, 1357 |
| BpiI | GAAGAC | 2 cut(s) | 590, 741 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 485, 517, 801, 833 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1127 |
| Bpu14I | TTCGAA | 2 cut(s) | 15, 1057 |
| BpuEI | CTTGAG | 1 cut(s) | 911 |
| BpuMI | CCSGG | 1 cut(s) | 192 |
| BsaHI | GRCGYC | 1 cut(s) | 444 |
| BsaJI | CCNNGG | 3 cut(s) | 921, 1155, 1223 |
| BsaWI | WCCGGW | 4 cut(s) | 215, 256, 593, 973 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1027, 1156 |
| Bse118I | RCCGGY | 1 cut(s) | 256 |
| Bse1I | ACTGG | 1 cut(s) | 1150 |
| Bse3DI | GCAATG | 2 cut(s) | 385, 951 |
| BseAI | TCCGGA | 1 cut(s) | 973 |
| BseBI | CCWGG | 1 cut(s) | 1098 |
| BseDI | CCNNGG | 3 cut(s) | 921, 1155, 1223 |
| BseGI | GGATG | 6 cut(s) | 129, 377, 759, 1036, 1050, 1233 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1027, 1156 |
| BseMI | GCAATG | 2 cut(s) | 385, 951 |
| BseMII | CTCAG | 2 cut(s) | 279, 482 |
| BseNI | ACTGG | 1 cut(s) | 1150 |
| BseRI | GAGGAG | 4 cut(s) | 261, 668, 763, 1032 |
| BseXI | GCAGC | 5 cut(s) | 124, 245, 407, 463, 914 |
| Bsh1236I | CGCG | 5 cut(s) | 458, 851, 923, 1157, 1176 |
| BshFI | GGCC | 1 cut(s) | 105 |
| BshTI | ACCGGT | 1 cut(s) | 256 |
| BsiHKAI | GWGCWC | 2 cut(s) | 528, 660 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1139 |
| BsiSI | CCGG | 5 cut(s) | 192, 216, 257, 594, 974 |
| BslFI | GGGAC | 2 cut(s) | 817, 912 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1027, 1156 |
| BsmAI | GTCTC | 5 cut(s) | 325, 668, 751, 975, 1142 |
| BsmBI | CGTCTC | 2 cut(s) | 668, 751 |
| BsmFI | GGGAC | 2 cut(s) | 817, 912 |
| BsnI | GGCC | 1 cut(s) | 105 |
| BsoBI | CYCGRG | 1 cut(s) | 1139 |
| Bsp119I | TTCGAA | 2 cut(s) | 15, 1057 |
| Bsp1286I | GDGCHC | 3 cut(s) | 528, 660, 1119 |
| Bsp13I | TCCGGA | 1 cut(s) | 973 |
| Bsp1407I | TGTACA | 1 cut(s) | 306 |
| Bsp143I | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| BspANI | GGCC | 1 cut(s) | 105 |
| BspCNI | CTCAG | 2 cut(s) | 278, 481 |
| BspEI | TCCGGA | 1 cut(s) | 973 |
| BspFNI | CGCG | 5 cut(s) | 458, 851, 923, 1157, 1176 |
| BspHI | TCATGA | 1 cut(s) | 1360 |
| BspLI | GGNNCC | 3 cut(s) | 226, 927, 928 |
| BspMAI | CTGCAG | 1 cut(s) | 397 |
| BspPI | GGATC | 2 cut(s) | 173, 226 |
| BspQI | GCTCTTC | 1 cut(s) | 951 |
| BspT104I | TTCGAA | 2 cut(s) | 15, 1057 |
| BsrBI | CCGCTC | 3 cut(s) | 431, 944, 1248 |
| BsrDI | GCAATG | 2 cut(s) | 385, 951 |
| BsrFI | RCCGGY | 1 cut(s) | 256 |
| BsrGI | TGTACA | 1 cut(s) | 306 |
| BsrI | ACTGG | 1 cut(s) | 1150 |
| BssAI | RCCGGY | 1 cut(s) | 256 |
| BssECI | CCNNGG | 3 cut(s) | 921, 1155, 1223 |
| BssMI | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| BssNI | GRCGYC | 1 cut(s) | 444 |
| BssSI | CACGAG | 2 cut(s) | 341, 1243 |
| BssT1I | CCWWGG | 1 cut(s) | 1223 |
| Bst2BI | CACGAG | 2 cut(s) | 341, 1243 |
| Bst2UI | CCWGG | 1 cut(s) | 1098 |
| Bst4CI | ACNGT | 5 cut(s) | 68, 125, 311, 1027, 1196 |
| Bst6I | CTCTTC | 2 cut(s) | 155, 951 |
| BstACI | GRCGYC | 1 cut(s) | 444 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 550 |
| BstAUI | TGTACA | 1 cut(s) | 306 |
| BstBI | TTCGAA | 2 cut(s) | 15, 1057 |
| BstC8I | GCNNGC | 3 cut(s) | 377, 433, 456 |
| BstDEI | CTNAG | 3 cut(s) | 265, 468, 1127 |
| BstDSI | CCRYGG | 2 cut(s) | 921, 1155 |
| BstF5I | GGATG | 6 cut(s) | 129, 377, 759, 1036, 1050, 1233 |
| BstFNI | CGCG | 5 cut(s) | 458, 851, 923, 1157, 1176 |
| BstKTI | GATC | 6 cut(s) | 181, 221, 353, 406, 747, 1218 |
| BstMAI | GTCTC | 5 cut(s) | 325, 668, 751, 975, 1142 |
| BstMBI | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 102, 203, 233, 441, 460, 550, 793, 1123 |
| BstNI | CCWGG | 1 cut(s) | 1098 |
| BstNSI | RCATGY | 1 cut(s) | 553 |
| BstSCI | CCNGG | 2 cut(s) | 190, 1096 |
| BstSFI | CTRYAG | 1 cut(s) | 393 |
| BstUI | CGCG | 5 cut(s) | 458, 851, 923, 1157, 1176 |
| BstV1I | GCAGC | 5 cut(s) | 124, 245, 407, 463, 914 |
| BstV2I | GAAGAC | 2 cut(s) | 590, 741 |
| BsuRI | GGCC | 1 cut(s) | 105 |
| BtgI | CCRYGG | 2 cut(s) | 921, 1155 |
| BtrI | CACGTC | 1 cut(s) | 761 |
| BtsCI | GGATG | 6 cut(s) | 129, 377, 759, 1036, 1050, 1233 |
| BtsI | GCAGTG | 1 cut(s) | 390 |
| BtsIMutI | CAGTG | 3 cut(s) | 316, 390, 1032 |
| Cac8I | GCNNGC | 3 cut(s) | 377, 433, 456 |
| CciI | TCATGA | 1 cut(s) | 1360 |
| Cfr10I | RCCGGY | 1 cut(s) | 256 |
| Cfr13I | GGNCC | 2 cut(s) | 926, 1147 |
| Cfr42I | CCGCGG | 2 cut(s) | 924, 1158 |
| CseI | GACGC | 2 cut(s) | 85, 433 |
| Csp6I | GTAC | 2 cut(s) | 307, 329 |
| CspAI | ACCGGT | 1 cut(s) | 256 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 471, 506 |
| CviAII | CATG | 4 cut(s) | 550, 707, 979, 1361 |
| CviQI | GTAC | 2 cut(s) | 307, 329 |
| DdeI | CTNAG | 3 cut(s) | 265, 468, 1127 |
| DpnI | GATC | 6 cut(s) | 180, 220, 352, 405, 746, 1217 |
| DpnII | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1016 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1016 |
| Eam1104I | CTCTTC | 2 cut(s) | 155, 951 |
| EarI | CTCTTC | 2 cut(s) | 155, 951 |
| Ecl136II | GAGCTC | 1 cut(s) | 658 |
| Eco130I | CCWWGG | 1 cut(s) | 1223 |
| Eco24I | GRGCYC | 2 cut(s) | 660, 1119 |
| Eco47I | GGWCC | 2 cut(s) | 926, 1147 |
| Eco53kI | GAGCTC | 1 cut(s) | 658 |
| Eco88I | CYCGRG | 1 cut(s) | 1139 |
| EcoICRI | GAGCTC | 1 cut(s) | 658 |
| EcoO109I | RGGNCCY | 1 cut(s) | 926 |
| EcoRII | CCWGG | 1 cut(s) | 1096 |
| EcoT14I | CCWWGG | 1 cut(s) | 1223 |
| EcoT22I | ATGCAT | 1 cut(s) | 555 |
| EcoT38I | GRGCYC | 2 cut(s) | 660, 1119 |
| ErhI | CCWWGG | 1 cut(s) | 1223 |
| Esp3I | CGTCTC | 2 cut(s) | 668, 751 |
| FaeI | CATG | 4 cut(s) | 553, 710, 982, 1364 |
| FaqI | GGGAC | 2 cut(s) | 817, 912 |
| FatI | CATG | 4 cut(s) | 549, 706, 978, 1360 |
| FauI | CCCGC | 3 cut(s) | 211, 916, 928 |
| FokI | GGATG | 6 cut(s) | 116, 384, 766, 1037, 1043, 1240 |
| FriOI | GRGCYC | 2 cut(s) | 660, 1119 |
| FspBI | CTAG | 6 cut(s) | 110, 614, 824, 836, 1224, 1402 |
| HaeIII | GGCC | 1 cut(s) | 105 |
| HapII | CCGG | 5 cut(s) | 192, 216, 257, 594, 974 |
| HgaI | GACGC | 2 cut(s) | 85, 433 |
| Hin1I | GRCGYC | 1 cut(s) | 444 |
| Hin1II | CATG | 4 cut(s) | 553, 710, 982, 1364 |
| HincII | GTYRAC | 1 cut(s) | 262 |
| HindII | GTYRAC | 1 cut(s) | 262 |
| HindIII | AAGCTT | 2 cut(s) | 72, 1124 |
| HpaI | GTTAAC | 1 cut(s) | 262 |
| HpaII | CCGG | 5 cut(s) | 192, 216, 257, 594, 974 |
| HphI | GGTGA | 4 cut(s) | 113, 692, 814, 1376 |
| Hpy166II | GTNNAC | 4 cut(s) | 115, 262, 689, 913 |
| Hpy188I | TCNGA | 6 cut(s) | 82, 178, 471, 778, 1072, 1281 |
| Hpy8I | GTNNAC | 4 cut(s) | 115, 262, 689, 913 |
| Hpy99I | CGWCG | 3 cut(s) | 101, 404, 449 |
| HpyAV | CCTTC | 6 cut(s) | 95, 192, 806, 1064, 1175, 1328 |
| HpyCH4III | ACNGT | 5 cut(s) | 68, 125, 311, 1027, 1196 |
| HpyCH4IV | ACGT | 3 cut(s) | 760, 1017, 1067 |
| HpyCH4V | TGCA | 6 cut(s) | 129, 390, 395, 553, 764, 1348 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 102, 203, 233, 441, 460, 550, 793, 1123 |
| HpyF3I | CTNAG | 3 cut(s) | 265, 468, 1127 |
| HpySE526I | ACGT | 3 cut(s) | 760, 1017, 1067 |
| Hsp92I | GRCGYC | 1 cut(s) | 444 |
| Hsp92II | CATG | 4 cut(s) | 553, 710, 982, 1364 |
| KflI | GGGWCCC | 1 cut(s) | 926 |
| Kpn2I | TCCGGA | 1 cut(s) | 973 |
| KspAI | GTTAAC | 1 cut(s) | 262 |
| KspI | CCGCGG | 2 cut(s) | 924, 1158 |
| Kzo9I | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| LguI | GCTCTTC | 1 cut(s) | 951 |
| LmnI | GCTCC | 5 cut(s) | 194, 224, 655, 663, 899 |
| Lsp1109I | GCAGC | 5 cut(s) | 124, 245, 407, 463, 914 |
| LweI | GCATC | 4 cut(s) | 138, 362, 562, 1357 |
| MaeI | CTAG | 6 cut(s) | 110, 614, 824, 836, 1224, 1402 |
| MaeII | ACGT | 3 cut(s) | 760, 1017, 1067 |
| MaeIII | GTNAC | 3 cut(s) | 85, 272, 1196 |
| MalI | GATC | 6 cut(s) | 180, 220, 352, 405, 746, 1217 |
| MbiI | CCGCTC | 3 cut(s) | 431, 944, 1248 |
| MboI | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| MfeI | CAATTG | 1 cut(s) | 324 |
| MhlI | GDGCHC | 3 cut(s) | 528, 660, 1119 |
| MluCI | AATT | 7 cut(s) | 161, 299, 324, 355, 557, 859, 1092 |
| MlyI | GAGTC | 5 cut(s) | 258, 764, 810, 839, 1099 |
| Mph1103I | ATGCAT | 1 cut(s) | 555 |
| MroI | TCCGGA | 1 cut(s) | 973 |
| MseI | TTAA | 3 cut(s) | 59, 261, 581 |
| MslI | CAYNNNNRTG | 2 cut(s) | 705, 1359 |
| MspA1I | CMGCKG | 3 cut(s) | 905, 923, 1157 |
| MspI | CCGG | 5 cut(s) | 192, 216, 257, 594, 974 |
| MspR9I | CCNGG | 2 cut(s) | 192, 1098 |
| MunI | CAATTG | 1 cut(s) | 324 |
| MvaI | CCWGG | 1 cut(s) | 1098 |
| MvnI | CGCG | 5 cut(s) | 458, 851, 923, 1157, 1176 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 102, 203, 233, 441, 460, 550, 793, 1123 |
| NciI | CCSGG | 1 cut(s) | 192 |
| NdeII | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| NlaIII | CATG | 4 cut(s) | 553, 710, 982, 1364 |
| NlaIV | GGNNCC | 3 cut(s) | 226, 927, 928 |
| NmuCI | GTSAC | 1 cut(s) | 85 |
| NsiI | ATGCAT | 1 cut(s) | 555 |
| NspI | RCATGY | 1 cut(s) | 553 |
| NspV | TTCGAA | 2 cut(s) | 15, 1057 |
| PaeR7I | CTCGAG | 1 cut(s) | 1139 |
| PagI | TCATGA | 1 cut(s) | 1360 |
| PciSI | GCTCTTC | 1 cut(s) | 951 |
| PfeI | GAWTC | 7 cut(s) | 20, 173, 610, 1035, 1262, 1282, 1342 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1027 |
| PfoI | TCCNGGA | 1 cut(s) | 1096 |
| PinAI | ACCGGT | 1 cut(s) | 256 |
| PleI | GAGTC | 5 cut(s) | 257, 764, 810, 838, 1099 |
| PpsI | GAGTC | 5 cut(s) | 257, 764, 810, 838, 1099 |
| PpuMI | RGGWCCY | 1 cut(s) | 926 |
| Psp124BI | GAGCTC | 1 cut(s) | 660 |
| Psp5II | RGGWCCY | 1 cut(s) | 926 |
| Psp6I | CCWGG | 1 cut(s) | 1096 |
| PspGI | CCWGG | 1 cut(s) | 1096 |
| PspN4I | GGNNCC | 3 cut(s) | 226, 927, 928 |
| PspPI | GGNCC | 2 cut(s) | 926, 1147 |
| PspPPI | RGGWCCY | 1 cut(s) | 926 |
| PstI | CTGCAG | 1 cut(s) | 397 |
| PvuII | CAGCTG | 1 cut(s) | 905 |
| RsaI | GTAC | 2 cut(s) | 308, 330 |
| RsaNI | GTAC | 2 cut(s) | 307, 329 |
| RseI | CAYNNNNRTG | 2 cut(s) | 705, 1359 |
| SacI | GAGCTC | 1 cut(s) | 660 |
| SacII | CCGCGG | 2 cut(s) | 924, 1158 |
| SapI | GCTCTTC | 1 cut(s) | 951 |
| SaqAI | TTAA | 3 cut(s) | 59, 261, 581 |
| Sau3AI | GATC | 6 cut(s) | 178, 218, 350, 403, 744, 1215 |
| Sau96I | GGNCC | 2 cut(s) | 926, 1147 |
| SchI | GAGTC | 5 cut(s) | 258, 764, 810, 839, 1099 |
| ScrFI | CCNGG | 2 cut(s) | 192, 1098 |
| SduI | GDGCHC | 3 cut(s) | 528, 660, 1119 |
| SfaNI | GCATC | 4 cut(s) | 138, 362, 562, 1357 |
| SfcI | CTRYAG | 1 cut(s) | 393 |
| Sfr274I | CTCGAG | 1 cut(s) | 1139 |
| Sfr303I | CCGCGG | 2 cut(s) | 924, 1158 |
| SfuI | TTCGAA | 2 cut(s) | 15, 1057 |
| SgrBI | CCGCGG | 2 cut(s) | 924, 1158 |
| SinI | GGWCC | 2 cut(s) | 926, 1147 |
| SlaI | CTCGAG | 1 cut(s) | 1139 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 705, 1359 |
| SmlI | CTYRAG | 2 cut(s) | 890, 1139 |
| SmoI | CTYRAG | 2 cut(s) | 890, 1139 |
| Sse9I | AATT | 7 cut(s) | 161, 299, 324, 355, 557, 859, 1092 |
| SspMI | CTAG | 6 cut(s) | 110, 614, 824, 836, 1224, 1402 |
| SstI | GAGCTC | 1 cut(s) | 660 |
| StyD4I | CCNGG | 2 cut(s) | 190, 1096 |
| StyI | CCWWGG | 1 cut(s) | 1223 |
| TaaI | ACNGT | 5 cut(s) | 68, 125, 311, 1027, 1196 |
| TaiI | ACGT | 3 cut(s) | 763, 1020, 1070 |
| TasI | AATT | 7 cut(s) | 161, 299, 324, 355, 557, 859, 1092 |
| TatI | WGTACW | 1 cut(s) | 306 |
| TauI | GCSGC | 5 cut(s) | 239, 428, 431, 959, 1160 |
| TfiI | GAWTC | 7 cut(s) | 20, 173, 610, 1035, 1262, 1282, 1342 |
| Tru1I | TTAA | 3 cut(s) | 59, 261, 581 |
| Tru9I | TTAA | 3 cut(s) | 59, 261, 581 |
| TscAI | CASTG | 3 cut(s) | 316, 397, 1032 |
| TseFI | GTSAC | 1 cut(s) | 85 |
| TseI | GCWGC | 5 cut(s) | 137, 233, 395, 451, 902 |
| Tsp45I | GTSAC | 1 cut(s) | 85 |
| TspDTI | ATGAA | 3 cut(s) | 533, 1244, 1355 |
| TspGWI | ACGGA | 2 cut(s) | 650, 674 |
| TspRI | CASTG | 3 cut(s) | 316, 397, 1032 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1027 |
| VpaK11BI | GGWCC | 2 cut(s) | 926, 1147 |
| XapI | RAATTY | 1 cut(s) | 161 |
| XbaI | TCTAGA | 1 cut(s) | 823 |
| XceI | RCATGY | 1 cut(s) | 553 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1028 |
| XhoI | CTCGAG | 1 cut(s) | 1139 |
| XmaJI | CCTAGG | 1 cut(s) | 1223 |
| XspI | CTAG | 6 cut(s) | 110, 614, 824, 836, 1224, 1402 |
| Zsp2I | ATGCAT | 1 cut(s) | 555 |