MD12G1091100.v1.1

Regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012041
Physical Location & Seq
Forward (+)
11524223 .. 11524886
664 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1091100.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGTAATGGGGGAAGGCCTAAATCTCGACGAGGCATTGGCATTACACTATGCAGTCGAAAATTGCAGCCGGGAAGTTGTGAAAGCGTTACTAGAACTTGGAGCAGGTGATGTGAACTACCCGGCAGGGCCAGTCGGAAAAACCCCGCTTCACATTGCTTCCGAAATGGTGTCTCCGGACATGGTGGCAGTCCTACTCGACCACCACGCCGACCCAAATGTCCGAACAGTCGATGGGGTTACTCCTCTGGACGTACTCCGAACCCTAACGTCAGAATTTCTATTCAAAGGGGCTGTCCCGGGACTAACCCACATCGAACCTAACAAGCTCAGACTCTGCCTTGAGCTGGTCCAGTCTGCCGCTCTCGTTCTTTCGCGCGAGGAAGGCAACAACAATGCAAACAATCCCAATTCTTCGAATTCCACGGCGATTTATCCGCCAATTAGCGATGATCACCATAGCAGTGGCAGCAGTGGCAGCAACATTGGGAATCTTAACTTGGATTCTAGATTGGTGTATTTGAATCTTGGAGCAACTCATCAAATGGGGTCTAGAAATATGGAGGGCCACGATCATCATAATAGGGTGCACAGTGGCTGTGACCCATCAACAATGTACCACCACTCTCACGACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009798 GO:0009838 GO:0009864 GO:0009867 GO:0009888 GO:0009908 GO:0009943 GO:0009944 GO:0009954 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010022 GO:0010033 GO:0010073 GO:0010227 GO:0010254 GO:0010432 GO:0010433 GO:0010434 GO:0010498 GO:0010582 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0022414 GO:0023052 GO:0030162 GO:0030163 GO:0031347 GO:0031349 GO:0031461 GO:0031625 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0042221 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048366 GO:0048367 GO:0048437 GO:0048439 GO:0048507 GO:0048518 GO:0048583 GO:0048584 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0080090 GO:0080134 GO:0090567 GO:0090698 GO:0098542 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1901701 GO:1902494 GO:1905392 GO:1905393 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank_2 Ank_3 BTB DUF3420

Protein Analysis

213

Amino Acids

22.84

Weight (kDa)

5.47

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 10 - 75 1.2e-10 Ankyrin repeats (3 copies)
Ank PF00023 46 - 76 6.4e-06 Ankyrin repeat
Ank_5 PF13857 48 - 85 5.3e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41370 AT3G57130
fragaria_vesca FvH4_6g25090
malus_domestica MD12G1091100.v1.1 MD14G1083700.v1.1
prunus_persica Prupe.7G046800_v2.0.a1
pyrus_communis pycom12g07650 pycom14g07290
rosa_chinensis RchiOBHm_Chr2g0124891
rosa_laevigata RLG00000018831
rosa_multiflora Rmu_sc0005075.1_g000013
rosa_roxburghii Rroxscaffold_2G00119550
rosa_rugosa Rorug02G0253100
rosa_samantha Rh2AG312800 Rh2BG321000 Rh2CG302300 Rh2DG338400
rosa_wichuraiana Rw0G021780 Rw2G025260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
AasI GACNNNNNNGTC 1 cut(s) 218
Acc36I ACCTGC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 362
AccII CGCG 2 cut(s) 376, 378
AccIII TCCGGA 1 cut(s) 175
AciI CCGC 3 cut(s) 146, 360, 437
AcsI RAATTY 2 cut(s) 275, 418
AfaI GTAC 2 cut(s) 255, 617
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 2 cut(s) 286, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 2 cut(s) 328, 346
AluI AGCT 2 cut(s) 328, 346
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 177
Alw44I GTGCAC 1 cut(s) 587
AlwNI CAGNNNCTG 2 cut(s) 336, 597
Ama87I CYCGRG 1 cut(s) 298
Aor13HI TCCGGA 1 cut(s) 175
AoxI GGCC 3 cut(s) 16, 128, 565
ApaLI GTGCAC 1 cut(s) 587
ApeKI GCWGC 3 cut(s) 66, 468, 477
ApoI RAATTY 2 cut(s) 275, 418
ArsI GACNNNNNNTTYG 2 cut(s) 279, 311
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 3 cut(s) 128, 349, 565
AsuC2I CCSGG 4 cut(s) 71, 122, 299, 300
AsuHPI GGTGA 2 cut(s) 119, 446
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 298
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 78, 480, 489
BccI CCATC 2 cut(s) 227, 613
BceAI ACGGC 1 cut(s) 441
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 4 cut(s) 71, 122, 299, 300
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 4 cut(s) 92, 507, 552, 637
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 4 cut(s) 67, 360, 469, 478
BlsI GCNGC 4 cut(s) 68, 361, 470, 479
Bme1390I CCNGG 4 cut(s) 71, 122, 299, 300
Bme18I GGWCC 1 cut(s) 349
BmeT110I CYCGRG 1 cut(s) 298
BmgT120I GGNCC 3 cut(s) 128, 349, 565
BmrFI CCNGG 4 cut(s) 71, 122, 299, 300
Bpu14I TTCGAA 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 4 cut(s) 71, 122, 299, 300
BsaJI CCNNGG 2 cut(s) 298, 423
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 131, 352
Bse3DI GCAATG 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 175
BseDI CCNNGG 2 cut(s) 298, 423
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 343
BseNI ACTGG 2 cut(s) 131, 352
BseRI GAGGAG 1 cut(s) 234
BseSI GKGCMC 1 cut(s) 591
BseXI GCAGC 3 cut(s) 78, 480, 489
Bsh1236I CGCG 2 cut(s) 376, 378
BshFI GGCC 3 cut(s) 18, 130, 567
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 298
BsiSI CCGG 4 cut(s) 70, 122, 176, 299
BslFI GGGAC 2 cut(s) 281, 315
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 2 cut(s) 281, 315
BsnI GGCC 3 cut(s) 18, 130, 567
BsoBI CYCGRG 1 cut(s) 298
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 591
Bsp13I TCCGGA 1 cut(s) 175
Bsp143I GATC 2 cut(s) 451, 571
BspACI CCGC 3 cut(s) 146, 360, 437
BspANI GGCC 3 cut(s) 18, 130, 567
BspCNI CTCAG 1 cut(s) 342
BspEI TCCGGA 1 cut(s) 175
BspFNI CGCG 2 cut(s) 376, 378
BspMI ACCTGC 1 cut(s) 95
BspT104I TTCGAA 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 2 cut(s) 131, 352
BssECI CCNNGG 2 cut(s) 298, 423
BssMI GATC 2 cut(s) 451, 571
Bst4CI ACNGT 2 cut(s) 229, 593
BstBI TTCGAA 1 cut(s) 416
BstDEI CTNAG 1 cut(s) 329
BstDSI CCRYGG 1 cut(s) 423
BstFNI CGCG 2 cut(s) 376, 378
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 2 cut(s) 454, 574
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 451, 571
BstMWI GCNNNNNNNGC 4 cut(s) 384, 468, 474, 477
BstSCI CCNGG 4 cut(s) 69, 120, 297, 298
BstSLI GKGCMC 1 cut(s) 591
BstUI CGCG 2 cut(s) 376, 378
BstV1I GCAGC 3 cut(s) 78, 480, 489
BstXI CCANNNNNNTGG 1 cut(s) 464
BsuRI GGCC 3 cut(s) 18, 130, 567
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 462
BtsI GCAGTG 2 cut(s) 469, 478
BtsIMutI CAGTG 3 cut(s) 469, 478, 598
BveI ACCTGC 1 cut(s) 95
CaiI CAGNNNCTG 2 cut(s) 336, 597
CfoI GCGC 1 cut(s) 378
Cfr13I GGNCC 3 cut(s) 128, 349, 565
Cfr9I CCCGGG 1 cut(s) 298
Csp6I GTAC 2 cut(s) 254, 616
CviAII CATG 1 cut(s) 181
CviJI RGCY 8 cut(s) 18, 69, 130, 293, 328, 346, 567, 597
CviKI_1 RGCY 8 cut(s) 18, 69, 130, 293, 328, 346, 567, 597
CviQI GTAC 2 cut(s) 254, 616
DdeI CTNAG 1 cut(s) 329
DpnI GATC 2 cut(s) 453, 573
DpnII GATC 2 cut(s) 451, 571
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
EciI GGCGGA 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 418
FaeI CATG 1 cut(s) 184
FaiI YATR 5 cut(s) 51, 182, 459, 560, 579
FaqI GGGAC 2 cut(s) 281, 315
FatI CATG 1 cut(s) 180
FauI CCCGC 1 cut(s) 153
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 4 cut(s) 67, 360, 469, 478
Fsp4HI GCNGC 4 cut(s) 67, 360, 469, 478
FspBI CTAG 4 cut(s) 92, 507, 552, 637
GlaI GCGC 1 cut(s) 377
GluI GCNGC 4 cut(s) 67, 360, 469, 478
HaeIII GGCC 3 cut(s) 18, 130, 567
HapII CCGG 4 cut(s) 70, 122, 176, 299
HhaI GCGC 1 cut(s) 378
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 4 cut(s) 333, 490, 503, 523
HpaII CCGG 4 cut(s) 70, 122, 176, 299
HphI GGTGA 2 cut(s) 119, 446
Hpy166II GTNNAC 2 cut(s) 115, 589
Hpy188I TCNGA 6 cut(s) 137, 163, 224, 260, 274, 332
Hpy188III TCNNGA 6 cut(s) 26, 176, 248, 507, 552, 629
Hpy8I GTNNAC 2 cut(s) 115, 589
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 8, 377
HpyCH4III ACNGT 2 cut(s) 229, 593
HpyCH4IV ACGT 2 cut(s) 252, 269
HpyCH4V TGCA 4 cut(s) 53, 66, 398, 589
HpyF10VI GCNNNNNNNGC 4 cut(s) 384, 468, 474, 477
HpyF3I CTNAG 1 cut(s) 329
HpySE526I ACGT 2 cut(s) 252, 269
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 1 cut(s) 376
Kpn2I TCCGGA 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 2 cut(s) 451, 571
LmnI GCTCC 2 cut(s) 101, 530
Lsp1109I GCAGC 3 cut(s) 78, 480, 489
MaeI CTAG 4 cut(s) 92, 507, 552, 637
MaeII ACGT 2 cut(s) 252, 269
MaeIII GTNAC 3 cut(s) 87, 238, 599
MalI GATC 2 cut(s) 453, 573
MbiI CCGCTC 1 cut(s) 362
MboI GATC 2 cut(s) 451, 571
MboII GAAGA 1 cut(s) 405
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 5 cut(s) 61, 275, 409, 418, 441
MlyI GAGTC 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 4 cut(s) 25, 255, 373, 556
MroI TCCGGA 1 cut(s) 175
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 1 cut(s) 462
MspI CCGG 4 cut(s) 70, 122, 176, 299
MspR9I CCNGG 4 cut(s) 71, 122, 299, 300
MvnI CGCG 2 cut(s) 376, 378
MwoI GCNNNNNNNGC 4 cut(s) 384, 468, 474, 477
NciI CCSGG 4 cut(s) 71, 122, 299, 300
NdeII GATC 2 cut(s) 451, 571
NlaIII CATG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 599
NspV TTCGAA 1 cut(s) 416
PaqCI CACCTGC 1 cut(s) 95
PceI AGGCCT 1 cut(s) 18
PfeI GAWTC 3 cut(s) 490, 503, 523
PkrI GCNGC 4 cut(s) 68, 361, 470, 479
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
PspPI GGNCC 3 cut(s) 128, 349, 565
PstNI CAGNNNCTG 2 cut(s) 336, 597
RsaI GTAC 2 cut(s) 255, 617
RsaNI GTAC 2 cut(s) 254, 616
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 4 cut(s) 67, 360, 469, 478
Sau3AI GATC 2 cut(s) 451, 571
Sau96I GGNCC 3 cut(s) 128, 349, 565
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 4 cut(s) 71, 122, 299, 300
SduI GDGCHC 1 cut(s) 591
SetI ASST 6 cut(s) 109, 255, 272, 322, 330, 348
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 1 cut(s) 349
SmaI CCCGGG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 5 cut(s) 61, 275, 409, 418, 441
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 3 cut(s) 146, 360, 437
SspMI CTAG 4 cut(s) 92, 507, 552, 637
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 4 cut(s) 69, 120, 297, 298
TaaI ACNGT 2 cut(s) 229, 593
TaiI ACGT 2 cut(s) 255, 272
TaqI TCGA 6 cut(s) 27, 57, 198, 231, 315, 416
TasI AATT 5 cut(s) 61, 275, 409, 418, 441
TauI GCSGC 1 cut(s) 362
TfiI GAWTC 3 cut(s) 490, 503, 523
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 3 cut(s) 469, 478, 598
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 3 cut(s) 66, 468, 477
Tsp45I GTSAC 1 cut(s) 599
TspMI CCCGGG 1 cut(s) 298
TspRI CASTG 3 cut(s) 469, 478, 598
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 2 cut(s) 275, 418
XbaI TCTAGA 2 cut(s) 506, 551
XmaI CCCGGG 1 cut(s) 298
XspI CTAG 4 cut(s) 92, 507, 552, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.