MD14G1083700.v1.1

Regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012041
Physical Location & Seq
Forward (+)
9980772 .. 9984094
3323 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1083700.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGAGCAGCCTTGAAGAGTCTCTAAGATCTCTGTCCTTAGACTACTTGAATCTGCTTATCAATGGTCAAGCCTTCAGTGACGTCACCTTCAGCGTCGAGGGTCGTCTCGTCCACGCCCACAGGTGCATCCTGGCAGCCCGGAGCCTCTTCTTCCGGAAATTCTTTTGTGGGCCTGACCCACCGTTGGGTCTAGACCCCTCGGGGTCCAGGATGAGTCCCGGTGGCACATCGTCATCGTATCACCGGGGGTCAGGTAGTTCACCGCAGCAGCAGCAGGTGATACCGGTGAACTCGGTGGGGTACGAGGTGTTCTTGTTGCTGTTGCAGTTCTTGTACAGTGGACAAGTCTCGATAGTGCCTCAGAAACATGAGCCGAGGCCTAATTGTGGAGAGAGAGGGTGTTGGCACACGCATTGCACCTCCGCCGTTGATCTTGCCCTTGACACTCTTGCTGCCGCTAGATCCTTTGGTGTTGAACAACTTGCATTGCTCACTCAGCAATTGGCTAGCATGGTGGAAAAGGCCTCCATTGACGATGTGATGAAAGTCCTATTAGCTTCCAGGAAACAAGACATGCATCAGCTTTGGACAACGTGCTCTCACCTTGTTGCCAAATCTGGCCTCCCACCAGAAGTCCTTGCCAAGCACCTCCCCATCGATATCGTGGCCAAAATCGAAGAGCTTCGTCTCAAATCCTCCATCGCCCGCCGTTCAATGATGCCTCATCACCACCATCATCACCACCATGACCTCGGGGCAGCTGCGGATCTTGAGGACCAGAAAATCCGTCGAATGAGGCGGGCATTGGACTCGTCAGATGTCGAACTTGTCAAGCTCATGGTAATGGGGGAAGGCCTAAATCTCGATGAGGCATTGGCATTACACTATGCAGTCGAAAATTGCAGCCGGGAAGTTGTGAAAGCGTTACTAGAACTTGGTGCAGCTGATGTAAACTACCCGGCTGGGCCAGCCGGCAAAACCCCGCTTCACATTGCTTCCGAAATGGTGTCTCCGGACATGGTGGCAGTCCTACTCGATCACCACGCTGACCCAAATGTCCGAACGGTCGATGGGGTTACTCCTCTGGACGTACTCCGAACCCTAACTTCAGATTTTCTATTCAAGGGGGCTGTCCCGGGACTAACCCACATTGAACCTAACAAGCTCAGACTCTGCCTTGAGCTCGTACAGTCCGCTGCTCTTGTTCTTTCACGCGAGGAAGGCAACAGCAATGCAAACAACAATCCTAATTCTTCGAATTCCACGGCGATTTACCCACCGCTAAGCGATGATCACCATAGCAGTGGCAGCAGTGGCAGCAACATTGGGAACCTTAACTTGGATTCTAGATTGGTGTATCTGAATCTTGGAGCAGCTCATCAAATGGGGTCTAGAAATATGGAGGGCCATGATCATCAAGATCATGATCATCATCATAGGGCGCAAAGTGGCTGTGACCCATCAACAATGTACCACCACTCTCACGACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009798 GO:0009838 GO:0009864 GO:0009867 GO:0009888 GO:0009908 GO:0009943 GO:0009944 GO:0009954 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010022 GO:0010033 GO:0010073 GO:0010227 GO:0010254 GO:0010432 GO:0010433 GO:0010434 GO:0010498 GO:0010582 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0022414 GO:0023052 GO:0030162 GO:0030163 GO:0031347 GO:0031349 GO:0031461 GO:0031625 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0042221 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048366 GO:0048367 GO:0048437 GO:0048439 GO:0048507 GO:0048518 GO:0048583 GO:0048584 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0080090 GO:0080134 GO:0090567 GO:0090698 GO:0098542 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1901701 GO:1902494 GO:1905392 GO:1905393 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank_2 Ank_3 BTB DUF3420

Protein Analysis

498

Amino Acids

54.39

Weight (kDa)

6.16

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 19 - 57 1.4e-07 BTB/POZ domain
DUF3420 PF11900 207 - 266 3.8e-12 Domain of unknown function (DUF3420)
Ank_2 PF12796 271 - 354 1.8e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 326 - 364 1.3e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41370 AT3G57130
fragaria_vesca FvH4_6g25090
malus_domestica MD12G1091100.v1.1 MD14G1083700.v1.1
prunus_persica Prupe.7G046800_v2.0.a1
pyrus_communis pycom12g07650 pycom14g07290
rosa_chinensis RchiOBHm_Chr2g0124891
rosa_laevigata RLG00000018831
rosa_multiflora Rmu_sc0005075.1_g000013
rosa_roxburghii Rroxscaffold_2G00119550
rosa_rugosa Rorug02G0253100
rosa_samantha Rh2AG312800 Rh2BG321000 Rh2CG302300 Rh2DG338400
rosa_wichuraiana Rw0G021780 Rw2G025260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 265
AasI GACNNNNNNGTC 1 cut(s) 1055
AatII GACGTC 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 265
AccII CGCG 1 cut(s) 1215
AccIII TCCGGA 2 cut(s) 153, 1012
AciI CCGC 9 cut(s) 263, 423, 456, 706, 764, 799, 983, 1194, 1280
AclWI GGATC 2 cut(s) 456, 774
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 2 cut(s) 158, 1258
AcuI CTGAAG 3 cut(s) 58, 73, 1092
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 5 cut(s) 302, 335, 1092, 1188, 1472
AfiI CCNNNNNNNGG 4 cut(s) 184, 185, 386, 1339
AgeI ACCGGT 1 cut(s) 283
AgsI TTSAA 6 cut(s) 14, 49, 476, 714, 1123, 1154
AjnI CCWGG 3 cut(s) 129, 206, 560
AjuI GAANNNNNNNTTGG 2 cut(s) 1322, 1354
AluBI AGCT 9 cut(s) 557, 583, 682, 761, 835, 944, 1165, 1183, 1376
AluI AGCT 9 cut(s) 557, 583, 682, 761, 835, 944, 1165, 1183, 1376
Alw21I GWGCWC 2 cut(s) 599, 1185
Alw26I GTCTC 5 cut(s) 24, 110, 352, 692, 1014
AlwI GGATC 2 cut(s) 456, 774
AlwNI CAGNNNCTG 1 cut(s) 1173
Ama87I CYCGRG 3 cut(s) 199, 752, 1135
Aor13HI TCCGGA 2 cut(s) 153, 1012
AoxI GGCC 8 cut(s) 170, 377, 522, 619, 666, 853, 965, 1405
ApoI RAATTY 2 cut(s) 158, 1258
AsiGI ACCGGT 1 cut(s) 283
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 1 cut(s) 1444
AspS9I GGNCC 5 cut(s) 170, 204, 775, 965, 1405
AsuC2I CCSGG 7 cut(s) 139, 219, 245, 908, 959, 1136, 1137
AsuII TTCGAA 1 cut(s) 1256
AsuNHI GCTAGC 1 cut(s) 506
AvaI CYCGRG 3 cut(s) 199, 752, 1135
AvaII GGWCC 2 cut(s) 204, 775
BalI TGGCCA 1 cut(s) 668
BanII GRGCYC 1 cut(s) 1185
Bbv12I GWGCWC 2 cut(s) 599, 1185
BccI CCATC 5 cut(s) 662, 707, 741, 1064, 1468
BceAI ACGGC 3 cut(s) 410, 693, 1281
BcgI CGANNNNNNTGC 2 cut(s) 792, 826
BciT130I CCWGG 3 cut(s) 131, 208, 562
BclI TGATCA 3 cut(s) 1291, 1411, 1426
BcnI CCSGG 7 cut(s) 139, 219, 245, 908, 959, 1136, 1137
BcoDI GTCTC 5 cut(s) 24, 110, 352, 692, 1014
BfaI CTAG 7 cut(s) 191, 459, 507, 929, 1347, 1392, 1492
BfuAI ACCTGC 1 cut(s) 265
BglII AGATCT 1 cut(s) 26
BlpI GCTNAGC 1 cut(s) 1283
Bme18I GGWCC 2 cut(s) 204, 775
BmeT110I CYCGRG 3 cut(s) 199, 752, 1135
BmgT120I GGNCC 5 cut(s) 170, 204, 775, 965, 1405
BmiI GGNNCC 3 cut(s) 143, 205, 1331
BmsI GCATC 3 cut(s) 135, 586, 708
BmtI GCTAGC 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 1283
Bpu14I TTCGAA 1 cut(s) 1256
BpuEI CTTGAG 2 cut(s) 791, 1199
BpuMI CCSGG 7 cut(s) 139, 219, 245, 908, 959, 1136, 1137
Bsa29I ATCGAT 1 cut(s) 657
BsaBI GATNNNNATC 2 cut(s) 1425, 1431
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 6 cut(s) 198, 244, 374, 751, 1135, 1263
BsaWI WCCGGW 3 cut(s) 153, 283, 1012
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 185, 386, 1339
Bse118I RCCGGY 2 cut(s) 283, 971
Bse3DI GCAATG 4 cut(s) 412, 485, 990, 1237
Bse8I GATNNNNATC 2 cut(s) 1425, 1431
BseAI TCCGGA 2 cut(s) 153, 1012
BseBI CCWGG 3 cut(s) 131, 208, 562
BseCI ATCGAT 1 cut(s) 657
BseDI CCNNGG 6 cut(s) 198, 244, 374, 751, 1135, 1263
BseGI GGATG 2 cut(s) 126, 216
BseJI GATNNNNATC 2 cut(s) 1425, 1431
BseLI CCNNNNNNNGG 4 cut(s) 184, 185, 386, 1339
BseMI GCAATG 4 cut(s) 412, 485, 990, 1237
BseMII CTCAG 3 cut(s) 374, 509, 1180
BseRI GAGGAG 1 cut(s) 1071
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 1 cut(s) 1215
Bsh1285I CGRYCG 1 cut(s) 1068
BshFI GGCC 8 cut(s) 172, 379, 524, 621, 668, 855, 967, 1407
BshTI ACCGGT 1 cut(s) 283
BshVI ATCGAT 1 cut(s) 657
BsiEI CGRYCG 1 cut(s) 1068
BsiHKAI GWGCWC 2 cut(s) 599, 1185
BsiHKCI CYCGRG 3 cut(s) 199, 752, 1135
BslFI GGGAC 3 cut(s) 201, 1118, 1152
BslI CCNNNNNNNGG 4 cut(s) 184, 185, 386, 1339
BsmAI GTCTC 5 cut(s) 24, 110, 352, 692, 1014
BsmBI CGTCTC 2 cut(s) 110, 692
BsmFI GGGAC 3 cut(s) 201, 1118, 1152
BsnI GGCC 8 cut(s) 172, 379, 524, 621, 668, 855, 967, 1407
BsoBI CYCGRG 3 cut(s) 199, 752, 1135
Bsp119I TTCGAA 1 cut(s) 1256
Bsp1286I GDGCHC 2 cut(s) 599, 1185
Bsp13I TCCGGA 2 cut(s) 153, 1012
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
Bsp1720I GCTNAGC 1 cut(s) 1283
BspACI CCGC 9 cut(s) 263, 423, 456, 706, 764, 799, 983, 1194, 1280
BspANI GGCC 8 cut(s) 172, 379, 524, 621, 668, 855, 967, 1407
BspCNI CTCAG 3 cut(s) 373, 508, 1179
BspDI ATCGAT 1 cut(s) 657
BspEI TCCGGA 2 cut(s) 153, 1012
BspFNI CGCG 1 cut(s) 1215
BspHI TCATGA 1 cut(s) 1423
BspLI GGNNCC 3 cut(s) 143, 205, 1331
BspMI ACCTGC 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 510
BspPI GGATC 2 cut(s) 456, 774
BspQI GCTCTTC 1 cut(s) 672
BspT104I TTCGAA 1 cut(s) 1256
BsrDI GCAATG 4 cut(s) 412, 485, 990, 1237
BsrFI RCCGGY 2 cut(s) 283, 971
BsrGI TGTACA 1 cut(s) 333
BssAI RCCGGY 2 cut(s) 283, 971
BssECI CCNNGG 6 cut(s) 198, 244, 374, 751, 1135, 1263
BssMI GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 3 cut(s) 131, 208, 562
Bst4CI ACNGT 4 cut(s) 183, 338, 1066, 1191
Bst6I CTCTTC 3 cut(s) 9, 152, 672
BstACI GRCGYC 1 cut(s) 81
BstAUI TGTACA 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 1256
BstC8I GCNNGC 5 cut(s) 508, 706, 801, 969, 973
BstDEI CTNAG 6 cut(s) 23, 37, 360, 495, 1166, 1283
BstDSI CCRYGG 1 cut(s) 1263
BstF5I GGATG 2 cut(s) 126, 216
BstFNI CGCG 1 cut(s) 1215
BstHHI GCGC 1 cut(s) 1444
BstKTI GATC 9 cut(s) 29, 433, 464, 769, 1039, 1294, 1414, 1423, 1429
BstMAI GTCTC 5 cut(s) 24, 110, 352, 692, 1014
BstMBI GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
BstMCI CGRYCG 1 cut(s) 1068
BstMWI GCNNNNNNNGC 7 cut(s) 271, 496, 968, 1221, 1308, 1314, 1317
BstNI CCWGG 3 cut(s) 131, 208, 562
BstNSI RCATGY 1 cut(s) 577
BstUI CGCG 1 cut(s) 1215
BstX2I RGATCY 3 cut(s) 26, 461, 766
BstXI CCANNNNNNTGG 1 cut(s) 1304
BstYI RGATCY 3 cut(s) 26, 461, 766
Bsu15I ATCGAT 1 cut(s) 657
BsuRI GGCC 8 cut(s) 172, 379, 524, 621, 668, 855, 967, 1407
BsuTUI ATCGAT 1 cut(s) 657
BtgI CCRYGG 1 cut(s) 1263
BtgZI GCGATG 2 cut(s) 685, 1302
BtsCI GGATG 2 cut(s) 126, 216
BtsI GCAGTG 2 cut(s) 1309, 1318
BtsIMutI CAGTG 4 cut(s) 82, 343, 1309, 1318
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 5 cut(s) 508, 706, 801, 969, 973
CaiI CAGNNNCTG 1 cut(s) 1173
CciI TCATGA 1 cut(s) 1423
CfoI GCGC 1 cut(s) 1444
Cfr10I RCCGGY 2 cut(s) 283, 971
Cfr13I GGNCC 5 cut(s) 170, 204, 775, 965, 1405
Cfr9I CCCGGG 1 cut(s) 1135
ClaI ATCGAT 1 cut(s) 657
CseI GACGC 1 cut(s) 82
Csp6I GTAC 5 cut(s) 301, 334, 1091, 1187, 1471
CspAI ACCGGT 1 cut(s) 283
CviAII CATG 8 cut(s) 368, 511, 574, 746, 838, 1018, 1409, 1424
CviQI GTAC 5 cut(s) 301, 334, 1091, 1187, 1471
DdeI CTNAG 6 cut(s) 23, 37, 360, 495, 1166, 1283
DpnI GATC 9 cut(s) 28, 432, 463, 768, 1038, 1293, 1413, 1422, 1428
DpnII GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
DrdI GACNNNNNNGTC 1 cut(s) 1055
DseDI GACNNNNNNGTC 1 cut(s) 1055
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 3 cut(s) 9, 152, 672
EarI CTCTTC 3 cut(s) 9, 152, 672
EciI GGCGGA 1 cut(s) 412
Ecl136II GAGCTC 1 cut(s) 1183
Eco147I AGGCCT 3 cut(s) 379, 524, 855
Eco24I GRGCYC 1 cut(s) 1185
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 204, 775
Eco53kI GAGCTC 1 cut(s) 1183
Eco57I CTGAAG 3 cut(s) 58, 73, 1092
Eco88I CYCGRG 3 cut(s) 199, 752, 1135
EcoICRI GAGCTC 1 cut(s) 1183
EcoRI GAATTC 1 cut(s) 1258
EcoRII CCWGG 3 cut(s) 129, 206, 560
EcoRV GATATC 1 cut(s) 661
EcoT22I ATGCAT 1 cut(s) 579
EcoT38I GRGCYC 1 cut(s) 1185
Esp3I CGTCTC 2 cut(s) 110, 692
FaeI CATG 8 cut(s) 371, 514, 577, 749, 841, 1021, 1412, 1427
FaqI GGGAC 3 cut(s) 201, 1118, 1152
FatI CATG 8 cut(s) 367, 510, 573, 745, 837, 1017, 1408, 1423
FauI CCCGC 3 cut(s) 713, 792, 990
FbaI TGATCA 3 cut(s) 1291, 1411, 1426
FokI GGATG 2 cut(s) 113, 223
FriOI GRGCYC 1 cut(s) 1185
FspBI CTAG 7 cut(s) 191, 459, 507, 929, 1347, 1392, 1492
GlaI GCGC 1 cut(s) 1443
GsaI CCCAGC 1 cut(s) 966
HaeIII GGCC 8 cut(s) 172, 379, 524, 621, 668, 855, 967, 1407
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 1444
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 8 cut(s) 371, 514, 577, 749, 841, 1021, 1412, 1427
Hin6I GCGC 1 cut(s) 1442
HinP1I GCGC 1 cut(s) 1442
HinfI GANTC 7 cut(s) 17, 49, 214, 809, 1170, 1343, 1363
Hpy166II GTNNAC 5 cut(s) 112, 260, 289, 341, 952
Hpy188I TCNGA 8 cut(s) 363, 817, 1000, 1061, 1097, 1111, 1169, 1362
Hpy8I GTNNAC 5 cut(s) 112, 260, 289, 341, 952
Hpy99I CGWCG 2 cut(s) 98, 792
HpyAV CCTTC 4 cut(s) 82, 97, 845, 1214
HpyCH4III ACNGT 4 cut(s) 183, 338, 1066, 1191
HpyCH4IV ACGT 3 cut(s) 81, 593, 1089
HpyCH4V TGCA 9 cut(s) 126, 325, 417, 485, 577, 890, 903, 941, 1235
HpyF10VI GCNNNNNNNGC 7 cut(s) 271, 496, 968, 1221, 1308, 1314, 1317
HpyF3I CTNAG 6 cut(s) 23, 37, 360, 495, 1166, 1283
HpySE526I ACGT 3 cut(s) 81, 593, 1089
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 8 cut(s) 371, 514, 577, 749, 841, 1021, 1412, 1427
HspAI GCGC 1 cut(s) 1442
Kpn2I TCCGGA 2 cut(s) 153, 1012
KroI GCCGGC 1 cut(s) 971
KroNI GCCGGC 1 cut(s) 973
Ksp22I TGATCA 3 cut(s) 1291, 1411, 1426
Kzo9I GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
LguI GCTCTTC 1 cut(s) 672
LmnI GCTCC 2 cut(s) 141, 1370
LweI GCATC 3 cut(s) 135, 586, 708
MaeI CTAG 7 cut(s) 191, 459, 507, 929, 1347, 1392, 1492
MaeII ACGT 3 cut(s) 81, 593, 1089
MaeIII GTNAC 5 cut(s) 77, 82, 924, 1075, 1454
MalI GATC 9 cut(s) 28, 432, 463, 768, 1038, 1293, 1413, 1422, 1428
MboI GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
MboII GAAGA 5 cut(s) 26, 139, 142, 689, 1245
MfeI CAATTG 1 cut(s) 500
MflI RGATCY 3 cut(s) 26, 461, 766
MhlI GDGCHC 2 cut(s) 599, 1185
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 6 cut(s) 158, 382, 500, 898, 1249, 1258
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 4 cut(s) 26, 223, 803, 1164
Mox20I TGGCCA 1 cut(s) 668
Mph1103I ATGCAT 1 cut(s) 579
MroI TCCGGA 2 cut(s) 153, 1012
MroNI GCCGGC 1 cut(s) 971
MroXI GAANNNNTTC 1 cut(s) 681
MscI TGGCCA 1 cut(s) 668
MseI TTAA 1 cut(s) 1335
MslI CAYNNNNRTG 3 cut(s) 744, 842, 1302
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 3 cut(s) 761, 944, 1196
MunI CAATTG 1 cut(s) 500
MvaI CCWGG 3 cut(s) 131, 208, 562
MvnI CGCG 1 cut(s) 1215
MwoI GCNNNNNNNGC 7 cut(s) 271, 496, 968, 1221, 1308, 1314, 1317
NaeI GCCGGC 1 cut(s) 973
NciI CCSGG 7 cut(s) 139, 219, 245, 908, 959, 1136, 1137
NdeII GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
NgoMIV GCCGGC 1 cut(s) 971
NheI GCTAGC 1 cut(s) 506
NlaIII CATG 8 cut(s) 371, 514, 577, 749, 841, 1021, 1412, 1427
NlaIV GGNNCC 3 cut(s) 143, 205, 1331
NmeAIII GCCGAG 1 cut(s) 399
NmuCI GTSAC 3 cut(s) 77, 82, 1454
NsiI ATGCAT 1 cut(s) 579
NspI RCATGY 1 cut(s) 577
NspV TTCGAA 1 cut(s) 1256
PagI TCATGA 1 cut(s) 1423
PaqCI CACCTGC 1 cut(s) 265
PceI AGGCCT 3 cut(s) 379, 524, 855
PciSI GCTCTTC 1 cut(s) 672
PdiI GCCGGC 1 cut(s) 973
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 3 cut(s) 49, 1343, 1363
PfoI TCCNGGA 2 cut(s) 206, 560
PinAI ACCGGT 1 cut(s) 283
PleI GAGTC 4 cut(s) 25, 222, 803, 1164
PpsI GAGTC 4 cut(s) 25, 222, 803, 1164
Psp124BI GAGCTC 1 cut(s) 1185
Psp6I CCWGG 3 cut(s) 129, 206, 560
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 3 cut(s) 129, 206, 560
PspN4I GGNNCC 3 cut(s) 143, 205, 1331
PspPI GGNCC 5 cut(s) 170, 204, 775, 965, 1405
PsrI GAACNNNNNNTAC 2 cut(s) 293, 325
PstNI CAGNNNCTG 1 cut(s) 1173
PsuI RGATCY 3 cut(s) 26, 461, 766
PvuII CAGCTG 2 cut(s) 761, 944
RsaI GTAC 5 cut(s) 302, 335, 1092, 1188, 1472
RsaNI GTAC 5 cut(s) 301, 334, 1091, 1187, 1471
RseI CAYNNNNRTG 3 cut(s) 744, 842, 1302
SacI GAGCTC 1 cut(s) 1185
SapI GCTCTTC 1 cut(s) 672
SaqAI TTAA 1 cut(s) 1335
Sau3AI GATC 9 cut(s) 26, 430, 461, 766, 1036, 1291, 1411, 1420, 1426
Sau96I GGNCC 5 cut(s) 170, 204, 775, 965, 1405
SchI GAGTC 4 cut(s) 26, 223, 803, 1164
SduI GDGCHC 2 cut(s) 599, 1185
SfaNI GCATC 3 cut(s) 135, 586, 708
SfuI TTCGAA 1 cut(s) 1256
SinI GGWCC 2 cut(s) 204, 775
SmaI CCCGGG 1 cut(s) 1137
SmiMI CAYNNNNRTG 3 cut(s) 744, 842, 1302
SmlI CTYRAG 2 cut(s) 770, 1178
SmoI CTYRAG 2 cut(s) 770, 1178
Sse9I AATT 6 cut(s) 158, 382, 500, 898, 1249, 1258
SseBI AGGCCT 3 cut(s) 379, 524, 855
SsiI CCGC 9 cut(s) 263, 423, 456, 706, 764, 799, 983, 1194, 1280
SspMI CTAG 7 cut(s) 191, 459, 507, 929, 1347, 1392, 1492
SstI GAGCTC 1 cut(s) 1185
StuI AGGCCT 3 cut(s) 379, 524, 855
TaaI ACNGT 4 cut(s) 183, 338, 1066, 1191
TaiI ACGT 3 cut(s) 84, 596, 1092
TasI AATT 6 cut(s) 158, 382, 500, 898, 1249, 1258
TatI WGTACW 1 cut(s) 333
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 3 cut(s) 49, 1343, 1363
Tru1I TTAA 1 cut(s) 1335
Tru9I TTAA 1 cut(s) 1335
TscAI CASTG 4 cut(s) 82, 343, 1309, 1318
TseFI GTSAC 3 cut(s) 77, 82, 1454
Tsp45I GTSAC 3 cut(s) 77, 82, 1454
TspDTI ATGAA 1 cut(s) 557
TspGWI ACGGA 1 cut(s) 776
TspMI CCCGGG 1 cut(s) 1135
TspRI CASTG 4 cut(s) 82, 343, 1309, 1318
VpaK11BI GGWCC 2 cut(s) 204, 775
XapI RAATTY 2 cut(s) 158, 1258
XbaI TCTAGA 3 cut(s) 190, 1346, 1391
XceI RCATGY 1 cut(s) 577
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
XmaI CCCGGG 1 cut(s) 1135
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 7 cut(s) 191, 459, 507, 929, 1347, 1392, 1492
ZraI GACGTC 1 cut(s) 82
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.