AT3G60070
ERF Family

Major facilitator superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005390
Physical Location & Seq
Reverse (-)
22183243 .. 22186657
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60070.3

Sequence Viewer

Length: 1377 bp
ATGACCGGAGGAAGTGAGGAGGATCCATTCACAAAACCCATTGGAAGATGGTCTGTCTTCTTCTATGGAGTAGGGCATATGCTTAATGACATTACTGCTTCTTGTTGGTTCACTTACCTCCTCCTATTCTTGACCCAAATTGGTCTCTCCCCTAGAGATGCAGCCATTGTTATGCTCTCTGGCCAAGTTGCTGATGCATTTGCTACTATCTTCACTGGTGAATTGATTGATAGGTTTGGACATTTCAAGATTTGGCATGCTGCAGGATCATTACTGGTTGCAATCTCGTTTTCCTCCGTTTTTGGAGGTTGTCTGCCTTGTTCGATCCTCCATAACGACTCTTTAACACTTGAAACCTTCTCCTACAGTATGTTTGCAGCTATATTTAACATTGGATGGGCTGCTACTCAAGTTTCCCATATGGCTATGGTTAATTGTATTTCATTGAACTCAACAAGCAGAGTTGCTTTAACAAGCTCTCGTAACGCGTTTACTATGGTTGCTAATCTTGGCTTATATGCGATTGCTTTAGTAGTATTCGGTGTTATTAAGGCTGGTTCAAAAGAAGATACCGAAACTCAGTATCGCTGGATTGCATATTCATCTATCACAATTGGATGCTGCTTTGTGGTCATATTTCTTATGGGGACAAAAGAGCCAAGGCTGAGGATAGATTTGAAAGAAACTAGCCGAGCGAGAATACCGTGGGTCTATTGGTTCCGGAAAATTCTGTACTATCAAGTTGCCATGGTTTACCTCCTCACTCGATTAGTATTGAATGTTTCTCAGGCATATCTTGCGTTCTTTGTTATCGATGATTTGCAAATGGATCAATCCGCTAAAGCTTTGGTTCCTGCAATTATCTATATCTGCAGCTTTGTTGTATCGGTTCTGCTTCAGGAGATTCCATGGAACGGGAAACGCTTGAAGGCGTATTATACAGCTGGTGGTATAATTTGGATATTCTGTGGTGCAGCAATTCTTCTATTGCCTAGAGACATAAGTTCTTTCATGTATGCGATATCTGTCTTTATCGGCATCGCGAATGCATTAATGATGGTAACAGCAATCAGTATGCAGAGTGTGTTGGTTGGTGCAGAAGTCGGTGGTTGCGCATTTGTTTGCGGCTCGTTGAGCTTCTTAGACAAGATGTCTTGTGGACTTGCTCTATATGTTCTTCAGTCACACCAAACTACTTCACCTAGAGGCCAACTAAACAACAACCAACAAAGTGTTTACCTTTCAGTTACAAGATACGGTTTAGGTCTTGTTCCAGCATTGTGCTCGTTTGTTGGTGTCGCTGTAACGTTCTTTATGGAGCTCGAAGCTGCTGGATCATTATCGAAGCCTCTGCTGCGTGAGCCGTTACTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_2

Protein Analysis

458

Amino Acids

50.33

Weight (kDa)

7.92

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_2 PF13347 21 - 442 3.3e-16 MFS/sugar transport protein
MFS_1 PF07690 25 - 370 1.8e-09 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1114
AccII CGCG 2 cut(s) 488, 1043
AccIII TCCGGA 1 cut(s) 720
AciI CCGC 2 cut(s) 837, 1125
AclI AACGTT 1 cut(s) 1307
AclWI GGATC 6 cut(s) 17, 30, 274, 319, 837, 1342
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 1 cut(s) 726
AcuI CTGAAG 2 cut(s) 881, 1163
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 1 cut(s) 914
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 7 cut(s) 247, 353, 448, 561, 679, 778, 928
AhdI GACNNNNNGTC 1 cut(s) 1150
AluBI AGCT 8 cut(s) 380, 477, 845, 876, 944, 1137, 1321, 1328
AluI AGCT 8 cut(s) 380, 477, 845, 876, 944, 1137, 1321, 1328
Alw21I GWGCWC 2 cut(s) 1286, 1323
Alw26I GTCTC 2 cut(s) 149, 990
AlwI GGATC 6 cut(s) 17, 30, 274, 319, 837, 1342
Aor13HI TCCGGA 1 cut(s) 720
AoxI GGCC 2 cut(s) 181, 1207
ApeKI GCWGC 9 cut(s) 161, 260, 377, 401, 621, 873, 974, 1328, 1354
ApoI RAATTY 1 cut(s) 726
AseI ATTAAT 1 cut(s) 1052
AspLEI GCGC 1 cut(s) 1115
AsuHPI GGTGA 2 cut(s) 230, 1191
BalI TGGCCA 1 cut(s) 183
BamHI GGATCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 1323
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 2 cut(s) 1286, 1323
BbvCI CCTCAGC 1 cut(s) 665
BbvI GCAGC 9 cut(s) 173, 247, 388, 389, 608, 885, 986, 1315, 1341
BccI CCATC 3 cut(s) 42, 390, 1051
BceAI ACGGC 1 cut(s) 1348
BcgI CGANNNNNNTGC 4 cut(s) 1266, 1300, 1333, 1367
BcoDI GTCTC 2 cut(s) 149, 990
BfaI CTAG 5 cut(s) 153, 687, 993, 1203, 1370
BfmI CTRYAG 3 cut(s) 261, 364, 871
BmeRI GACNNNNNGTC 1 cut(s) 1150
BmiI GGNNCC 3 cut(s) 24, 719, 852
BmsI GCATC 4 cut(s) 148, 184, 608, 1047
BpiI GAAGAC 1 cut(s) 49
Bpu10I CCTNAGC 1 cut(s) 665
BpuEI CTTGAG 1 cut(s) 393
Bsa29I ATCGAT 1 cut(s) 813
BsaBI GATNNNNATC 1 cut(s) 1339
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 4 cut(s) 659, 704, 747, 908
BsaWI WCCGGW 2 cut(s) 5, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 914
Bse1I ACTGG 2 cut(s) 220, 279
Bse8I GATNNNNATC 1 cut(s) 1339
BseAI TCCGGA 1 cut(s) 720
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 4 cut(s) 659, 704, 747, 908
BseGI GGATG 2 cut(s) 401, 623
BseJI GATNNNNATC 1 cut(s) 1339
BseLI CCNNNNNNNGG 1 cut(s) 914
BseMII CTCAG 3 cut(s) 593, 656, 800
BseNI ACTGG 2 cut(s) 220, 279
BseRI GAGGAG 3 cut(s) 32, 110, 749
BseXI GCAGC 9 cut(s) 173, 247, 388, 389, 608, 885, 986, 1315, 1341
BsgI GTGCAG 2 cut(s) 993, 1116
Bsh1236I CGCG 2 cut(s) 488, 1043
BshFI GGCC 2 cut(s) 183, 1209
BshVI ATCGAT 1 cut(s) 813
BsiHKAI GWGCWC 2 cut(s) 1286, 1323
BsiSI CCGG 2 cut(s) 6, 721
BslFI GGGAC 1 cut(s) 661
BslI CCNNNNNNNGG 1 cut(s) 914
BsmAI GTCTC 2 cut(s) 149, 990
BsmFI GGGAC 1 cut(s) 661
BsmI GAATGC 1 cut(s) 1051
BsnI GGCC 2 cut(s) 183, 1209
Bso31I GGTCTC 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 1286, 1323
Bsp13I TCCGGA 1 cut(s) 720
Bsp143I GATC 5 cut(s) 22, 266, 324, 829, 1334
Bsp19I CCATGG 2 cut(s) 747, 908
Bsp68I TCGCGA 1 cut(s) 1043
BspACI CCGC 2 cut(s) 837, 1125
BspANI GGCC 2 cut(s) 183, 1209
BspCNI CTCAG 3 cut(s) 592, 657, 799
BspDI ATCGAT 1 cut(s) 813
BspEI TCCGGA 1 cut(s) 720
BspFNI CGCG 2 cut(s) 488, 1043
BspLI GGNNCC 3 cut(s) 24, 719, 852
BspMAI CTGCAG 2 cut(s) 265, 875
BspPI GGATC 6 cut(s) 17, 30, 274, 319, 837, 1342
BspTNI GGTCTC 1 cut(s) 149
BsrI ACTGG 2 cut(s) 220, 279
BssECI CCNNGG 4 cut(s) 659, 704, 747, 908
BssMI GATC 5 cut(s) 22, 266, 324, 829, 1334
BssT1I CCWWGG 3 cut(s) 659, 747, 908
Bst4CI ACNGT 3 cut(s) 368, 705, 1259
BstAPI GCANNNNNTGC 1 cut(s) 797
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 4 cut(s) 579, 665, 786, 1141
BstDSI CCRYGG 3 cut(s) 704, 747, 908
BstF5I GGATG 2 cut(s) 401, 623
BstFNI CGCG 2 cut(s) 488, 1043
BstHHI GCGC 1 cut(s) 1115
BstKTI GATC 5 cut(s) 25, 269, 327, 832, 1337
BstMAI GTCTC 2 cut(s) 149, 990
BstMBI GATC 5 cut(s) 22, 266, 324, 829, 1334
BstMWI GCNNNNNNNGC 4 cut(s) 797, 1134, 1354, 1360
BstNSI RCATGY 1 cut(s) 260
BstSFI CTRYAG 3 cut(s) 261, 364, 871
BstUI CGCG 2 cut(s) 488, 1043
BstV1I GCAGC 9 cut(s) 173, 247, 388, 389, 608, 885, 986, 1315, 1341
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 813
BsuRI GGCC 2 cut(s) 183, 1209
BsuTUI ATCGAT 1 cut(s) 813
BtgI CCRYGG 3 cut(s) 704, 747, 908
BtgZI GCGATG 1 cut(s) 1024
BtsCI GGATG 2 cut(s) 401, 623
BtsIMutI CAGTG 1 cut(s) 213
BtuMI TCGCGA 1 cut(s) 1043
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 1 cut(s) 1115
ClaI ATCGAT 1 cut(s) 813
Csp6I GTAC 1 cut(s) 733
CviAII CATG 4 cut(s) 257, 748, 909, 1012
CviQI GTAC 1 cut(s) 733
DdeI CTNAG 4 cut(s) 579, 665, 786, 1141
DpnI GATC 5 cut(s) 24, 268, 326, 831, 1336
DpnII GATC 5 cut(s) 22, 266, 324, 829, 1334
DriI GACNNNNNGTC 1 cut(s) 1150
EaeI YGGCCR 1 cut(s) 181
Eam1105I GACNNNNNGTC 1 cut(s) 1150
Ecl136II GAGCTC 1 cut(s) 1321
Eco130I CCWWGG 3 cut(s) 659, 747, 908
Eco24I GRGCYC 1 cut(s) 1323
Eco31I GGTCTC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 1023
Eco53kI GAGCTC 1 cut(s) 1321
Eco57I CTGAAG 2 cut(s) 881, 1163
EcoICRI GAGCTC 1 cut(s) 1321
EcoRV GATATC 1 cut(s) 1023
EcoT14I CCWWGG 3 cut(s) 659, 747, 908
EcoT22I ATGCAT 2 cut(s) 199, 1051
EcoT38I GRGCYC 1 cut(s) 1323
ErhI CCWWGG 3 cut(s) 659, 747, 908
FaeI CATG 4 cut(s) 260, 751, 912, 1015
FaqI GGGAC 1 cut(s) 661
FatI CATG 4 cut(s) 256, 747, 908, 1011
FauNDI CATATG 2 cut(s) 78, 420
FokI GGATG 2 cut(s) 408, 630
FriOI GRGCYC 1 cut(s) 1323
FspBI CTAG 5 cut(s) 153, 687, 993, 1203, 1370
FspI TGCGCA 1 cut(s) 1114
GlaI GCGC 1 cut(s) 1114
HaeIII GGCC 2 cut(s) 183, 1209
HapII CCGG 2 cut(s) 6, 721
HhaI GCGC 1 cut(s) 1115
Hin1II CATG 4 cut(s) 260, 751, 912, 1015
Hin6I GCGC 1 cut(s) 1113
HinP1I GCGC 1 cut(s) 1113
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 2 cut(s) 338, 904
HpaII CCGG 2 cut(s) 6, 721
HphI GGTGA 2 cut(s) 230, 1191
Hpy166II GTNNAC 5 cut(s) 111, 492, 754, 1160, 1237
Hpy188III TCNNGA 5 cut(s) 130, 247, 721, 899, 1042
Hpy8I GTNNAC 5 cut(s) 111, 492, 754, 1160, 1237
HpyAV CCTTC 2 cut(s) 367, 922
HpyCH4III ACNGT 3 cut(s) 368, 705, 1259
HpyCH4IV ACGT 1 cut(s) 1307
HpyF10VI GCNNNNNNNGC 4 cut(s) 797, 1134, 1354, 1360
HpyF3I CTNAG 4 cut(s) 579, 665, 786, 1141
HpySE526I ACGT 1 cut(s) 1307
Hsp92II CATG 4 cut(s) 260, 751, 912, 1015
HspAI GCGC 1 cut(s) 1113
Kpn2I TCCGGA 1 cut(s) 720
Kzo9I GATC 5 cut(s) 22, 266, 324, 829, 1334
LmnI GCTCC 1 cut(s) 1318
Lsp1109I GCAGC 9 cut(s) 173, 247, 388, 389, 608, 885, 986, 1315, 1341
LweI GCATC 4 cut(s) 148, 184, 608, 1047
MaeI CTAG 5 cut(s) 153, 687, 993, 1203, 1370
MaeII ACGT 1 cut(s) 1307
MaeIII GTNAC 6 cut(s) 482, 1060, 1182, 1246, 1303, 1365
MalI GATC 5 cut(s) 24, 268, 326, 831, 1336
MboI GATC 5 cut(s) 22, 266, 324, 829, 1334
MboII GAAGA 7 cut(s) 49, 52, 57, 202, 578, 974, 1169
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 2 cut(s) 1286, 1323
MlsI TGGCCA 1 cut(s) 183
MluCI AATT 8 cut(s) 138, 221, 433, 612, 726, 858, 954, 978
MluI ACGCGT 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 183
MlyI GAGTC 1 cut(s) 332
Mox20I TGGCCA 1 cut(s) 183
Mph1103I ATGCAT 2 cut(s) 199, 1051
MroI TCCGGA 1 cut(s) 720
MscI TGGCCA 1 cut(s) 183
MseI TTAA 7 cut(s) 84, 344, 387, 432, 470, 549, 1052
MslI CAYNNNNRTG 1 cut(s) 170
Msp20I TGGCCA 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 944
MspI CCGG 2 cut(s) 6, 721
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 1 cut(s) 1051
MvnI CGCG 2 cut(s) 488, 1043
MwoI GCNNNNNNNGC 4 cut(s) 797, 1134, 1354, 1360
NcoI CCATGG 2 cut(s) 747, 908
NdeI CATATG 2 cut(s) 78, 420
NdeII GATC 5 cut(s) 22, 266, 324, 829, 1334
NlaIII CATG 4 cut(s) 260, 751, 912, 1015
NlaIV GGNNCC 3 cut(s) 24, 719, 852
NmeAIII GCCGAG 1 cut(s) 716
NmuCI GTSAC 1 cut(s) 1182
NruI TCGCGA 1 cut(s) 1043
NsbI TGCGCA 1 cut(s) 1114
NsiI ATGCAT 2 cut(s) 199, 1051
NspI RCATGY 1 cut(s) 260
PaeI GCATGC 1 cut(s) 260
PctI GAATGC 1 cut(s) 1051
PfeI GAWTC 1 cut(s) 904
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PshBI ATTAAT 1 cut(s) 1052
Psp124BI GAGCTC 1 cut(s) 1323
Psp1406I AACGTT 1 cut(s) 1307
PspN4I GGNNCC 3 cut(s) 24, 719, 852
PstI CTGCAG 2 cut(s) 265, 875
PsuI RGATCY 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 944
RruI TCGCGA 1 cut(s) 1043
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
RseI CAYNNNNRTG 1 cut(s) 170
SacI GAGCTC 1 cut(s) 1323
SaqAI TTAA 7 cut(s) 84, 344, 387, 432, 470, 549, 1052
Sau3AI GATC 5 cut(s) 22, 266, 324, 829, 1334
SchI GAGTC 1 cut(s) 332
SduI GDGCHC 2 cut(s) 1286, 1323
SfaNI GCATC 4 cut(s) 148, 184, 608, 1047
SfcI CTRYAG 3 cut(s) 261, 364, 871
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
SphI GCATGC 1 cut(s) 260
Sse9I AATT 8 cut(s) 138, 221, 433, 612, 726, 858, 954, 978
SsiI CCGC 2 cut(s) 837, 1125
SspMI CTAG 5 cut(s) 153, 687, 993, 1203, 1370
SstI GAGCTC 1 cut(s) 1323
StyI CCWWGG 3 cut(s) 659, 747, 908
TaaI ACNGT 3 cut(s) 368, 705, 1259
TaiI ACGT 1 cut(s) 1310
TaqI TCGA 5 cut(s) 323, 766, 813, 1323, 1343
TasI AATT 8 cut(s) 138, 221, 433, 612, 726, 858, 954, 978
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 1128
TfiI GAWTC 1 cut(s) 904
Tru1I TTAA 7 cut(s) 84, 344, 387, 432, 470, 549, 1052
Tru9I TTAA 7 cut(s) 84, 344, 387, 432, 470, 549, 1052
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 1 cut(s) 1182
TseI GCWGC 9 cut(s) 161, 260, 377, 401, 621, 873, 974, 1328, 1354
Tsp45I GTSAC 1 cut(s) 1182
TspDTI ATGAA 3 cut(s) 432, 591, 1000
TspGWI ACGGA 1 cut(s) 286
TspRI CASTG 1 cut(s) 220
VspI ATTAAT 1 cut(s) 1052
XapI RAATTY 1 cut(s) 726
XceI RCATGY 1 cut(s) 260
XspI CTAG 5 cut(s) 153, 687, 993, 1203, 1370
Zsp2I ATGCAT 2 cut(s) 199, 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.