Rroxscaffold_4G00332430
ERF Family

Major facilitator superfamily domain-containing protein 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005390
Physical Location & Seq
Forward (+)
66563572 .. 66574077
10506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00332430.1

Sequence Viewer

Length: 1368 bp
ATGCATAATGTTACTGAAGATGATGATGATTCAGTCACAAAGCCCCTTGGAAGGTGTTCTGTCATGTATTATGGCGTGGGCCATATGCTCAATGATATCACTGCTTCCTGTTGGTTTACATATCTCTTGTTATTCTTGACAGATATTGGACTATCCCCCAGGGATGCTGCCACTGTAATGCTATCCGGTCAAATAGCTGATGGATTTGCAACCATATTTGCTGGCGAACTGATAGACAGATTTGGACATTTCAAATTGTGGCATGGTGCAGGCTCTATTTTGGTTGCTGTTTCATTTTCTTCCGTTTTTGGTGGTTGTGTACCGTGTAAAATTTTTGGTACCTCTTCACCAGCATTGCGAACTGTTGGTTACAGCATTTTTGCTGCTATCTTTAATGTGGGTTGGGCAGCTACCCAGGTCTCACACATGTCTATGCTGAATTGCATTACACTGAATTCAACAAGCAGAGTGGTGCTCGCTAGCTGTCGGAATGCTTTTACTATGGTTGCCAACCTGAGCTTGTATGCGGTTGCTTTTATAATCTTTGCTGTCACGATAGCAAGAACACATGCTGATATTGAAAATCAGTACCGCTGGATTGCATATTTGTCAATCTTCATAGGATGCTGCTTTGTTGGTATCTTTCAACTCGGCACCAAAGAGCCAAGATTAAAGGTAGGTGTACAAGGAAGTAATTCTTCAAGGATTCCATGGACGTACTGGTTCAAGAGAGTGCTATATTATCAGGTTGCTCTTGTTTATGTGCTTACCAGACTAGTGGTTAATGTTTCACAGGCATATCTTGCATTCTATGTTATAGATGATCTGCAGATGGCACAATCGGCTAAAGCTCTGGTTCCTGCGATCATCTACATAAGTAGCTTCCTCGTATCTGTGATATTGCAGGAGATACAATGGACTGGCCGATTCTTGAAGGCCTACTACTCAGCTGGAGCTATTATTTGGATATTTTGTGGTGCAGGGATCCTGCTTTTACCTAGAAGCTTGAGTGCTTTCATGTACATTATATCTATATTTATTGGCATAGCAAATGCTTTAATGATGGTGACCGGAGTAAGCATGCAAAGTGTTTTAATTGATACAGATCTCAATGGCTGTGCATTTGTTTGTGGATCATTCAGCTTCTTGGACAAAATCTCATGTGGGCTTGCTTTATTTGTTCTTCAGTCGTATCAAAGTGATTCAGTCATACAGAATATCCATTCTCATCATGTCTACATATCAGTTACGAGATTCGGGTTGGGTCTTGTTCCGGCATTTTGTGCACTTGTGTCAGTGGCTGTTACATACACGATGAATTTCCACCCTGCTCGCTGCAAACCTCTAATGGAGCCTCTATTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_2

Protein Analysis

455

Amino Acids

50.01

Weight (kDa)

8.33

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_2 PF13347 24 - 438 5.8e-14 MFS/sugar transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 2 cut(s) 338, 653
AccI GTMKAC 1 cut(s) 1236
AciI CCGC 2 cut(s) 527, 592
AclWI GGATC 3 cut(s) 979, 992, 1141
AcoI YGGCCR 1 cut(s) 922
AcsI RAATTY 3 cut(s) 330, 454, 1318
AcuI CTGAAG 2 cut(s) 36, 1169
AfaI GTAC 6 cut(s) 321, 340, 590, 684, 719, 1022
AfiI CCNNNNNNNGG 1 cut(s) 51
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 7 cut(s) 253, 459, 581, 647, 702, 727, 934
AhlI ACTAGT 1 cut(s) 775
AjnI CCWGG 2 cut(s) 158, 414
Alw21I GWGCWC 2 cut(s) 477, 1288
Alw26I GTCTC 1 cut(s) 424
Alw44I GTGCAC 1 cut(s) 1284
AlwI GGATC 3 cut(s) 979, 992, 1141
AlwNI CAGNNNCTG 1 cut(s) 1301
AoxI GGCC 3 cut(s) 79, 922, 936
ApaLI GTGCAC 1 cut(s) 1284
ApeKI GCWGC 5 cut(s) 167, 383, 407, 627, 1335
ApoI RAATTY 3 cut(s) 330, 454, 1318
Asp700I GAANNNNTTC 2 cut(s) 55, 694
Asp718I GGTACC 1 cut(s) 338
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 339, 1078
AsuNHI GCTAGC 1 cut(s) 479
BaeGI GKGCMC 1 cut(s) 1288
BamHI GGATCC 1 cut(s) 984
BanI GGYRCC 2 cut(s) 338, 653
BarI GAAGNNNNNNTAC 2 cut(s) 926, 958
Bbv12I GWGCWC 2 cut(s) 477, 1288
BbvI GCAGC 5 cut(s) 154, 370, 419, 614, 1322
BccI CCATC 3 cut(s) 194, 826, 1057
BciT130I CCWGG 2 cut(s) 160, 416
BcoDI GTCTC 1 cut(s) 424
BcuI ACTAGT 1 cut(s) 775
BfaI CTAG 3 cut(s) 480, 776, 999
BfmI CTRYAG 1 cut(s) 827
BglII AGATCT 1 cut(s) 1105
BisI GCNGC 5 cut(s) 168, 384, 408, 628, 1336
BlsI GCNGC 5 cut(s) 169, 385, 409, 629, 1337
Bme1390I CCNGG 2 cut(s) 160, 416
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 5 cut(s) 340, 655, 858, 986, 1353
BmrFI CCNGG 2 cut(s) 160, 416
BmsI GCATC 2 cut(s) 154, 614
BmtI GCTAGC 1 cut(s) 483
BplI GAGNNNNNCTC 2 cut(s) 459, 491
BpmI CTGGAG 1 cut(s) 972
Bpu10I CCTNAGC 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 1027
BsaBI GATNNNNATC 1 cut(s) 1104
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 5 cut(s) 46, 158, 159, 414, 710
BsaWI WCCGGW 2 cut(s) 185, 1070
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse1I ACTGG 2 cut(s) 725, 925
Bse3DI GCAATG 1 cut(s) 353
Bse8I GATNNNNATC 1 cut(s) 1104
BseBI CCWGG 2 cut(s) 160, 416
BseDI CCNNGG 5 cut(s) 46, 158, 159, 414, 710
BseGI GGATG 2 cut(s) 169, 629
BseJI GATNNNNATC 1 cut(s) 1104
BseLI CCNNNNNNNGG 1 cut(s) 51
BseMI GCAATG 1 cut(s) 353
BseMII CTCAG 2 cut(s) 506, 960
BseNI ACTGG 2 cut(s) 725, 925
BseSI GKGCMC 1 cut(s) 1288
BseXI GCAGC 5 cut(s) 154, 370, 419, 614, 1322
BsgI GTGCAG 2 cut(s) 288, 999
BshFI GGCC 3 cut(s) 81, 924, 938
BshNI GGYRCC 2 cut(s) 338, 653
BsiHKAI GWGCWC 2 cut(s) 477, 1288
BsiSI CCGG 3 cut(s) 186, 1071, 1274
BslI CCNNNNNNNGG 1 cut(s) 51
BsmAI GTCTC 1 cut(s) 424
BsmI GAATGC 2 cut(s) 496, 806
BsnI GGCC 3 cut(s) 81, 924, 938
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 477, 1288
Bsp1407I TGTACA 2 cut(s) 682, 1020
Bsp143I GATC 5 cut(s) 823, 864, 984, 1105, 1133
Bsp19I CCATGG 1 cut(s) 710
BspACI CCGC 2 cut(s) 527, 592
BspANI GGCC 3 cut(s) 81, 924, 938
BspCNI CTCAG 2 cut(s) 507, 959
BspLI GGNNCC 5 cut(s) 340, 655, 858, 986, 1353
BspMAI CTGCAG 1 cut(s) 831
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 3 cut(s) 979, 992, 1141
BspT107I GGYRCC 2 cut(s) 338, 653
BspTNI GGTCTC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 353
BsrGI TGTACA 2 cut(s) 682, 1020
BsrI ACTGG 2 cut(s) 725, 925
BssECI CCNNGG 5 cut(s) 46, 158, 159, 414, 710
BssMI GATC 5 cut(s) 823, 864, 984, 1105, 1133
BssT1I CCWWGG 2 cut(s) 46, 710
Bst2UI CCWGG 2 cut(s) 160, 416
Bst4CI ACNGT 3 cut(s) 175, 324, 364
Bst6I CTCTTC 1 cut(s) 349
BstAPI GCANNNNNTGC 2 cut(s) 803, 1283
BstAUI TGTACA 2 cut(s) 682, 1020
BstC8I GCNNGC 7 cut(s) 223, 271, 477, 481, 1082, 1170, 1333
BstDEI CTNAG 2 cut(s) 515, 946
BstDSI CCRYGG 1 cut(s) 710
BstEII GGTNACC 1 cut(s) 1066
BstF5I GGATG 2 cut(s) 169, 629
BstKTI GATC 5 cut(s) 826, 867, 987, 1108, 1136
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 5 cut(s) 823, 864, 984, 1105, 1133
BstMWI GCNNNNNNNGC 3 cut(s) 803, 842, 1283
BstNI CCWGG 2 cut(s) 160, 416
BstNSI RCATGY 3 cut(s) 430, 572, 1084
BstPI GGTNACC 1 cut(s) 1066
BstSCI CCNGG 2 cut(s) 158, 414
BstSFI CTRYAG 1 cut(s) 827
BstSLI GKGCMC 1 cut(s) 1288
BstV1I GCAGC 5 cut(s) 154, 370, 419, 614, 1322
BstX2I RGATCY 2 cut(s) 984, 1105
BstXI CCANNNNNNTGG 1 cut(s) 778
BstYI RGATCY 2 cut(s) 984, 1105
BsuRI GGCC 3 cut(s) 81, 924, 938
BtgI CCRYGG 1 cut(s) 710
BtsCI GGATG 2 cut(s) 169, 629
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 4 cut(s) 99, 171, 449, 1302
Cac8I GCNNGC 7 cut(s) 223, 271, 477, 481, 1082, 1170, 1333
CaiI CAGNNNCTG 1 cut(s) 1301
Cfr13I GGNCC 1 cut(s) 79
Csp6I GTAC 6 cut(s) 320, 339, 589, 683, 718, 1021
CspCI CAANNNNNGTGG 2 cut(s) 450, 485
CviAII CATG 9 cut(s) 64, 263, 427, 569, 711, 1018, 1081, 1161, 1232
CviQI GTAC 6 cut(s) 320, 339, 589, 683, 718, 1021
DdeI CTNAG 2 cut(s) 515, 946
DpnI GATC 5 cut(s) 825, 866, 986, 1107, 1135
DpnII GATC 5 cut(s) 823, 864, 984, 1105, 1133
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 349
Eco130I CCWWGG 2 cut(s) 46, 710
Eco147I AGGCCT 1 cut(s) 938
Eco31I GGTCTC 1 cut(s) 424
Eco32I GATATC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 36, 1169
Eco91I GGTNACC 1 cut(s) 1066
EcoO65I GGTNACC 1 cut(s) 1066
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 158, 414
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 46, 710
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 46, 710
FaeI CATG 9 cut(s) 67, 266, 430, 572, 714, 1021, 1084, 1164, 1235
FalI AAGNNNNNCTT 2 cut(s) 682, 714
FatI CATG 9 cut(s) 63, 262, 426, 568, 710, 1017, 1080, 1160, 1231
FauNDI CATATG 1 cut(s) 84
FblI GTMKAC 1 cut(s) 1236
Fnu4HI GCNGC 5 cut(s) 168, 384, 408, 628, 1336
FokI GGATG 2 cut(s) 176, 636
Fsp4HI GCNGC 5 cut(s) 168, 384, 408, 628, 1336
FspBI CTAG 3 cut(s) 480, 776, 999
GluI GCNGC 5 cut(s) 168, 384, 408, 628, 1336
GsuI CTGGAG 1 cut(s) 972
HaeIII GGCC 3 cut(s) 81, 924, 938
HapII CCGG 3 cut(s) 186, 1071, 1274
Hin1II CATG 9 cut(s) 67, 266, 430, 572, 714, 1021, 1084, 1164, 1235
HindIII AAGCTT 1 cut(s) 1003
HinfI GANTC 5 cut(s) 29, 706, 927, 1202, 1254
HpaII CCGG 3 cut(s) 186, 1071, 1274
HphI GGTGA 2 cut(s) 339, 1078
Hpy166II GTNNAC 5 cut(s) 117, 320, 683, 1237, 1286
Hpy188I TCNGA 1 cut(s) 489
Hpy188III TCNNGA 4 cut(s) 136, 553, 727, 931
Hpy8I GTNNAC 5 cut(s) 117, 320, 683, 1237, 1286
HpyAV CCTTC 2 cut(s) 45, 928
HpyCH4III ACNGT 3 cut(s) 175, 324, 364
HpyCH4IV ACGT 1 cut(s) 716
HpyF10VI GCNNNNNNNGC 3 cut(s) 803, 842, 1283
HpyF3I CTNAG 2 cut(s) 515, 946
HpySE526I ACGT 1 cut(s) 716
Hsp92II CATG 9 cut(s) 67, 266, 430, 572, 714, 1021, 1084, 1164, 1235
KpnI GGTACC 1 cut(s) 342
Kzo9I GATC 5 cut(s) 823, 864, 984, 1105, 1133
LmnI GCTCC 2 cut(s) 953, 1351
Lsp1109I GCAGC 5 cut(s) 154, 370, 419, 614, 1322
LweI GCATC 2 cut(s) 154, 614
MaeI CTAG 3 cut(s) 480, 776, 999
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 7 cut(s) 10, 34, 368, 550, 1066, 1246, 1303
MalI GATC 5 cut(s) 825, 866, 986, 1107, 1135
MboI GATC 5 cut(s) 823, 864, 984, 1105, 1133
MboII GAAGA 6 cut(s) 29, 291, 336, 607, 690, 1175
MflI RGATCY 2 cut(s) 984, 1105
MhlI GDGCHC 2 cut(s) 477, 1288
MluCI AATT 7 cut(s) 254, 330, 439, 454, 694, 1095, 1318
MmeI TCCRAC 1 cut(s) 467
MnlI CCTC 4 cut(s) 352, 896, 1353, 1365
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 55, 694
MseI TTAA 5 cut(s) 393, 671, 783, 1058, 1094
MslI CAYNNNNRTG 2 cut(s) 176, 431
MspA1I CMGCKG 2 cut(s) 594, 950
MspI CCGG 3 cut(s) 186, 1071, 1274
MspR9I CCNGG 2 cut(s) 160, 416
Mva1269I GAATGC 2 cut(s) 496, 806
MvaI CCWGG 2 cut(s) 160, 416
MwoI GCNNNNNNNGC 3 cut(s) 803, 842, 1283
NcoI CCATGG 1 cut(s) 710
NdeI CATATG 1 cut(s) 84
NdeII GATC 5 cut(s) 823, 864, 984, 1105, 1133
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 9 cut(s) 67, 266, 430, 572, 714, 1021, 1084, 1164, 1235
NlaIV GGNNCC 5 cut(s) 340, 655, 858, 986, 1353
NmeAIII GCCGAG 1 cut(s) 630
NmuCI GTSAC 3 cut(s) 34, 550, 1066
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 430, 572, 1084
PaeI GCATGC 1 cut(s) 1084
PasI CCCWGGG 1 cut(s) 159
PceI AGGCCT 1 cut(s) 938
PciI ACATGT 1 cut(s) 426
PctI GAATGC 2 cut(s) 496, 806
PdmI GAANNNNTTC 2 cut(s) 55, 694
PfeI GAWTC 5 cut(s) 29, 706, 927, 1202, 1254
PkrI GCNGC 5 cut(s) 169, 385, 409, 629, 1337
PscI ACATGT 1 cut(s) 426
PsiI TTATAA 1 cut(s) 539
Psp6I CCWGG 2 cut(s) 158, 414
PspEI GGTNACC 1 cut(s) 1066
PspGI CCWGG 2 cut(s) 158, 414
PspN4I GGNNCC 5 cut(s) 340, 655, 858, 986, 1353
PspPI GGNCC 1 cut(s) 79
PstI CTGCAG 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 1301
PsuI RGATCY 2 cut(s) 984, 1105
PvuII CAGCTG 1 cut(s) 950
RsaI GTAC 6 cut(s) 321, 340, 590, 684, 719, 1022
RsaNI GTAC 6 cut(s) 320, 339, 589, 683, 718, 1021
RseI CAYNNNNRTG 2 cut(s) 176, 431
SaqAI TTAA 5 cut(s) 393, 671, 783, 1058, 1094
SatI GCNGC 5 cut(s) 168, 384, 408, 628, 1336
Sau3AI GATC 5 cut(s) 823, 864, 984, 1105, 1133
Sau96I GGNCC 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 160, 416
SduI GDGCHC 2 cut(s) 477, 1288
SfaNI GCATC 2 cut(s) 154, 614
SfcI CTRYAG 1 cut(s) 827
SmiMI CAYNNNNRTG 2 cut(s) 176, 431
SmlI CTYRAG 1 cut(s) 1006
SmoI CTYRAG 1 cut(s) 1006
SpeI ACTAGT 1 cut(s) 775
SphI GCATGC 1 cut(s) 1084
Sse9I AATT 7 cut(s) 254, 330, 439, 454, 694, 1095, 1318
SseBI AGGCCT 1 cut(s) 938
SsiI CCGC 2 cut(s) 527, 592
SspMI CTAG 3 cut(s) 480, 776, 999
StuI AGGCCT 1 cut(s) 938
StyD4I CCNGG 2 cut(s) 158, 414
StyI CCWWGG 2 cut(s) 46, 710
TaaI ACNGT 3 cut(s) 175, 324, 364
TaiI ACGT 1 cut(s) 719
TasI AATT 7 cut(s) 254, 330, 439, 454, 694, 1095, 1318
TatI WGTACW 2 cut(s) 682, 1020
TfiI GAWTC 5 cut(s) 29, 706, 927, 1202, 1254
Tru1I TTAA 5 cut(s) 393, 671, 783, 1058, 1094
Tru9I TTAA 5 cut(s) 393, 671, 783, 1058, 1094
TscAI CASTG 4 cut(s) 106, 178, 456, 1302
TseFI GTSAC 3 cut(s) 34, 550, 1066
TseI GCWGC 5 cut(s) 167, 383, 407, 627, 1335
Tsp45I GTSAC 3 cut(s) 34, 550, 1066
TspDTI ATGAA 4 cut(s) 282, 607, 1006, 1331
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 4 cut(s) 106, 178, 456, 1302
VneI GTGCAC 1 cut(s) 1284
XapI RAATTY 3 cut(s) 330, 454, 1318
XceI RCATGY 3 cut(s) 430, 572, 1084
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XmiI GTMKAC 1 cut(s) 1236
XmnI GAANNNNTTC 2 cut(s) 55, 694
XspI CTAG 3 cut(s) 480, 776, 999
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.