FvH4_c5g00010
ERF Family

Major facilitator superfamily domain-containing protein 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
contig_5
OG0005390
Physical Location & Seq
Reverse (-)
1479 .. 14808
13330 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_c5g00010.t2

Sequence Viewer

Length: 801 bp
ATGCATAATGTTACTGAAGATGATGATGATGATTCAGTCACCAAGCCCCTTGGAAGGTGTTCTGTCATGTATTATGGCGTGGGCCACATGCTCAATGATATTACTGCTTCCTGCTGGTTTACATACCTCTTGTTATTCTTGACAGATATTGGACTATCCCCCAGGGATGCTGCCACTGTAATGTTATCTGGTCAAATAGCTGATGGATTTGCAACCATATCTGCTGGCGAACTGATAGACAGATTTGGACATTTCAAATTGTGGCATGGTGCAGGCTCTGTGTTGGTTGCTGTTTCATTTTCTTCCGTGTTTGGTGGTTGCATACCATGTAAAATTTTCGGTACCTCTTCACCAGCATTACGAACTGTTGGTTACAGCATTTTTGCTGCTATCTTCAATGTGGGTTGGGCAGCTACCCAGGTTTCACACATGTCCATGCTGAATTGTATTACGTTGAACTCAACAAGCAGAGTGGTGCTCGCTAGCTGTCGGAATGCTTTTACTATGGTTGCCAACCTCGGCTTGTATGCGGTCGCTTTTATAATCTTTGGTGTCACGATAGCAAGAACACATGCCGATATTGAAAATCAGTACCGCTGGATTGCATATTTGTCAATCTTCATAGGATGCTGCTTTGTCGGTATCTTTCAATTCGGCACCAAAGAGCCAAGATTAAAGGTAGGCGTACAAGGAAGTAATTCTTCAAGGATTGCAATGGACTGGACGATTCTTGAAGGCCTACTACTCAGCTGGAGCTATTATTTGGATATTTTGTGGCGCAGGGATCCTGCTTTTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_2

Protein Analysis

267

Amino Acids

29.2

Weight (kDa)

6.28

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_2 PF13347 25 - 442 8.8e-15 MFS/sugar transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 341, 656
AciI CCGC 2 cut(s) 530, 595
AclWI GGATC 2 cut(s) 779, 792
AcsI RAATTY 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 3 cut(s) 343, 593, 687
AfiI CCNNNNNNNGG 1 cut(s) 54
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 7 cut(s) 256, 397, 457, 584, 650, 705, 734
AjnI CCWGG 2 cut(s) 161, 417
AluBI AGCT 5 cut(s) 200, 413, 486, 750, 756
AluI AGCT 5 cut(s) 200, 413, 486, 750, 756
Alw21I GWGCWC 1 cut(s) 480
AlwI GGATC 2 cut(s) 779, 792
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 2 cut(s) 82, 736
ApeKI GCWGC 4 cut(s) 170, 386, 410, 630
ApoI RAATTY 1 cut(s) 333
Asp700I GAANNNNTTC 2 cut(s) 58, 697
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 1 cut(s) 780
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 31, 342
AsuNHI GCTAGC 1 cut(s) 482
BamHI GGATCC 1 cut(s) 784
BanI GGYRCC 2 cut(s) 341, 656
BarI GAAGNNNNNNTAC 2 cut(s) 726, 758
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 4 cut(s) 157, 373, 422, 617
BccI CCATC 1 cut(s) 197
BciT130I CCWGG 2 cut(s) 163, 419
BfaI CTAG 2 cut(s) 483, 799
BisI GCNGC 4 cut(s) 171, 387, 411, 631
BlsI GCNGC 4 cut(s) 172, 388, 412, 632
Bme1390I CCNGG 2 cut(s) 163, 419
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 3 cut(s) 343, 658, 786
BmrFI CCNGG 2 cut(s) 163, 419
BmsI GCATC 2 cut(s) 157, 617
BmtI GCTAGC 1 cut(s) 486
BplI GAGNNNNNCTC 2 cut(s) 462, 494
BpmI CTGGAG 1 cut(s) 772
BsaJI CCNNGG 5 cut(s) 49, 161, 162, 417, 517
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse1I ACTGG 1 cut(s) 725
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 2 cut(s) 163, 419
BseDI CCNNGG 5 cut(s) 49, 161, 162, 417, 517
BseGI GGATG 2 cut(s) 172, 632
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 1 cut(s) 760
BseNI ACTGG 1 cut(s) 725
BseXI GCAGC 4 cut(s) 157, 373, 422, 617
BsgI GTGCAG 1 cut(s) 291
Bsh1285I CGRYCG 1 cut(s) 534
BshFI GGCC 2 cut(s) 84, 738
BshNI GGYRCC 2 cut(s) 341, 656
BsiEI CGRYCG 1 cut(s) 534
BsiHKAI GWGCWC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 54
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 2 cut(s) 84, 738
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 1 cut(s) 784
BspACI CCGC 2 cut(s) 530, 595
BspANI GGCC 2 cut(s) 84, 738
BspCNI CTCAG 1 cut(s) 759
BspLI GGNNCC 3 cut(s) 343, 658, 786
BspOI GCTAGC 1 cut(s) 486
BspPI GGATC 2 cut(s) 779, 792
BspT107I GGYRCC 2 cut(s) 341, 656
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 1 cut(s) 725
BssECI CCNNGG 5 cut(s) 49, 161, 162, 417, 517
BssMI GATC 1 cut(s) 784
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 2 cut(s) 163, 419
Bst4CI ACNGT 2 cut(s) 178, 367
Bst6I CTCTTC 1 cut(s) 352
BstC8I GCNNGC 4 cut(s) 226, 274, 480, 484
BstDEI CTNAG 1 cut(s) 746
BstF5I GGATG 2 cut(s) 172, 632
BstHHI GCGC 1 cut(s) 780
BstKTI GATC 1 cut(s) 787
BstMBI GATC 1 cut(s) 784
BstMCI CGRYCG 1 cut(s) 534
BstNI CCWGG 2 cut(s) 163, 419
BstNSI RCATGY 3 cut(s) 91, 433, 575
BstSCI CCNGG 2 cut(s) 161, 417
BstV1I GCAGC 4 cut(s) 157, 373, 422, 617
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BsuRI GGCC 2 cut(s) 84, 738
BtsCI GGATG 2 cut(s) 172, 632
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 4 cut(s) 226, 274, 480, 484
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 1 cut(s) 780
Cfr13I GGNCC 1 cut(s) 82
Csp6I GTAC 3 cut(s) 342, 592, 686
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 7 cut(s) 67, 88, 266, 327, 430, 436, 572
CviQI GTAC 3 cut(s) 342, 592, 686
DdeI CTNAG 1 cut(s) 746
DpnI GATC 1 cut(s) 786
DpnII GATC 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 49
Eco147I AGGCCT 1 cut(s) 738
Eco57I CTGAAG 1 cut(s) 36
EcoRII CCWGG 2 cut(s) 161, 417
EcoT14I CCWWGG 1 cut(s) 49
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 7 cut(s) 70, 91, 269, 330, 433, 439, 575
FalI AAGNNNNNCTT 2 cut(s) 685, 717
FatI CATG 7 cut(s) 66, 87, 265, 326, 429, 435, 571
Fnu4HI GCNGC 4 cut(s) 171, 387, 411, 631
FokI GGATG 2 cut(s) 179, 639
Fsp4HI GCNGC 4 cut(s) 171, 387, 411, 631
FspBI CTAG 2 cut(s) 483, 799
GlaI GCGC 1 cut(s) 779
GluI GCNGC 4 cut(s) 171, 387, 411, 631
GsuI CTGGAG 1 cut(s) 772
HaeIII GGCC 2 cut(s) 84, 738
HhaI GCGC 1 cut(s) 780
Hin1II CATG 7 cut(s) 70, 91, 269, 330, 433, 439, 575
Hin6I GCGC 1 cut(s) 778
HinP1I GCGC 1 cut(s) 778
HinfI GANTC 2 cut(s) 32, 727
HphI GGTGA 2 cut(s) 31, 342
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 1 cut(s) 492
Hpy188III TCNNGA 3 cut(s) 139, 556, 731
Hpy8I GTNNAC 1 cut(s) 120
HpyAV CCTTC 2 cut(s) 48, 728
HpyCH4III ACNGT 2 cut(s) 178, 367
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 6 cut(s) 4, 212, 272, 321, 605, 713
HpyF3I CTNAG 1 cut(s) 746
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 7 cut(s) 70, 91, 269, 330, 433, 439, 575
HspAI GCGC 1 cut(s) 778
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 1 cut(s) 784
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 4 cut(s) 157, 373, 422, 617
LweI GCATC 2 cut(s) 157, 617
MaeI CTAG 2 cut(s) 483, 799
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 4 cut(s) 10, 37, 371, 553
MalI GATC 1 cut(s) 786
MboI GATC 1 cut(s) 784
MboII GAAGA 6 cut(s) 29, 294, 339, 385, 610, 693
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 5 cut(s) 257, 333, 442, 650, 697
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 3 cut(s) 137, 355, 527
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 58, 697
MseI TTAA 1 cut(s) 674
MslI CAYNNNNRTG 2 cut(s) 179, 434
MspA1I CMGCKG 2 cut(s) 597, 750
MspR9I CCNGG 2 cut(s) 163, 419
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 2 cut(s) 163, 419
NdeII GATC 1 cut(s) 784
NheI GCTAGC 1 cut(s) 482
NlaIII CATG 7 cut(s) 70, 91, 269, 330, 433, 439, 575
NlaIV GGNNCC 3 cut(s) 343, 658, 786
NmeAIII GCCGAG 1 cut(s) 498
NmuCI GTSAC 2 cut(s) 37, 553
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 91, 433, 575
PasI CCCWGGG 1 cut(s) 162
PceI AGGCCT 1 cut(s) 738
PciI ACATGT 1 cut(s) 429
PctI GAATGC 1 cut(s) 499
PdmI GAANNNNTTC 2 cut(s) 58, 697
PfeI GAWTC 2 cut(s) 32, 727
PkrI GCNGC 4 cut(s) 172, 388, 412, 632
PscI ACATGT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 542
Psp6I CCWGG 2 cut(s) 161, 417
PspGI CCWGG 2 cut(s) 161, 417
PspN4I GGNNCC 3 cut(s) 343, 658, 786
PspPI GGNCC 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 784
PvuII CAGCTG 1 cut(s) 750
RsaI GTAC 3 cut(s) 343, 593, 687
RsaNI GTAC 3 cut(s) 342, 592, 686
RseI CAYNNNNRTG 2 cut(s) 179, 434
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 4 cut(s) 171, 387, 411, 631
Sau3AI GATC 1 cut(s) 784
Sau96I GGNCC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 163, 419
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 2 cut(s) 157, 617
SmiMI CAYNNNNRTG 2 cut(s) 179, 434
Sse9I AATT 5 cut(s) 257, 333, 442, 650, 697
SseBI AGGCCT 1 cut(s) 738
SsiI CCGC 2 cut(s) 530, 595
SspMI CTAG 2 cut(s) 483, 799
StuI AGGCCT 1 cut(s) 738
StyD4I CCNGG 2 cut(s) 161, 417
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 178, 367
TaiI ACGT 1 cut(s) 455
TasI AATT 5 cut(s) 257, 333, 442, 650, 697
TfiI GAWTC 2 cut(s) 32, 727
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 2 cut(s) 37, 553
TseI GCWGC 4 cut(s) 170, 386, 410, 630
Tsp45I GTSAC 2 cut(s) 37, 553
TspDTI ATGAA 2 cut(s) 285, 610
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 1 cut(s) 181
XapI RAATTY 1 cut(s) 333
XceI RCATGY 3 cut(s) 91, 433, 575
XmnI GAANNNNTTC 2 cut(s) 58, 697
XspI CTAG 2 cut(s) 483, 799
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.