RLG00000030819
ERF Family

Major facilitator superfamily domain-containing protein 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005390
Physical Location & Seq
Forward (+)
68068276 .. 68078765
10490 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030819

Sequence Viewer

Length: 1305 bp
ATGTATTATGGCGTGGGCCATATGCTCAATGATATCACTGCTTCCTGTTGGTTTACATATCTCTTGTTATTCTTGACAGATATTGGACTATCCCCCAGGGATGCTGCCACTGTAATGCTATCTGGTCAAATAGCTGATGGATTTGCAACCATATTTGCTGGCGAACTGATAGACAGATTTGGACATTTCAAATTGTGGCATGGTGCAGGCTCTATTTTGGTTGCCGTTTCATTTTCTTCTGTTTTTGGTGGTTGCGTACCGTGTAAAATTTTTGGTACCTCTTCACCAGCATTGCGAACTGTTGGTTACAGCATTTTTGCTGCTATCTTTAATGTGGGTTGGGCAGCTACCCAGGTCTCACACATGTCTATGCTGAATTGCATTACGCTGAATTCAACAAGCAGAGTGGTGCTCGCTAGCTGTCGGAATGCTTTTACTATGGTTGCCAACCTGAGCTTGTATGCGGTTGCTTTTATAATCTTTGCTATCACGATAGCAAGAACACATGCCGATATTGAAAATCAGTACCGCTGGATTGCATATTTGTCAATCTTCATAGGATGCTGCTTTGTTGGTATCTTTCAACTCGGCACCAAAGAGCCAAGATTAAAGGTAGGTGTACAAGGAAGTAATTCTTCAAGGATTCCATGGACGTACTGGTTCAAGAGAGTGCTATATTATCAGGTTGCTCTTGTTTATGTGCTTACCAGACTAGTGGTTAATGTTTCACAGGCATATCTTGCATTCTATGTTATAGATGATCTGCAGATGGCACAATCGGCTAAAGCTCTGGTTCCTGCGATCATCTACATAAGTAGCTTCGTCGTATCTGTGATATTGCAGGAGATACAATGGACTGGCGGATTCTTGAAGGCCTACTACTCAGCTGGAGCTATTATTTGGATATTTTGTGGTGCAGGGATCCTGCTTCTACCTAGAAGCTTGAGTGCTTTCATGTACATTATATCTATATTTATTGGCATAGCAAATGCTTTAATGATGGTGACCGGAGTAAGCATGCAAAGTGTTTTAATTGATACAGATCTCAATGGCTGTGCATTTGTTTGTGGATCATTCAGCTTCTTGGACAAAATCTCATGTGGGCTTGCTTTATTTGTTCTTCAGTCGTATCAAAGTGATTCAGTCATACAGAATATCCATTCTAATCATGTCTACATATCAGTTACGAGATTCGGGTTGGGTCTTGTTCCGGCATTTTGTTCACTTGTATCAGTGGCTGTTACATACTCGATGAACCTCCACTCTGCTCGCTGCAAGCCTCTAATGGAGCCTCTATTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_2

Protein Analysis

435

Amino Acids

47.55

Weight (kDa)

8.63

Isoelectric Point (pI)

31.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_2 PF13347 3 - 419 1.2e-14 MFS/sugar transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 275, 590
AccI GTMKAC 1 cut(s) 1173
AciI CCGC 3 cut(s) 464, 529, 861
AclWI GGATC 3 cut(s) 916, 929, 1078
AcsI RAATTY 2 cut(s) 267, 391
AcuI CTGAAG 1 cut(s) 1106
AfaI GTAC 6 cut(s) 258, 277, 527, 621, 656, 959
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 7 cut(s) 190, 396, 518, 584, 639, 664, 871
AhlI ACTAGT 1 cut(s) 712
AjnI CCWGG 2 cut(s) 95, 351
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 3 cut(s) 916, 929, 1078
AlwNI CAGNNNCTG 1 cut(s) 1238
AoxI GGCC 2 cut(s) 16, 873
ApeKI GCWGC 5 cut(s) 104, 320, 344, 564, 1272
ApoI RAATTY 2 cut(s) 267, 391
Asp700I GAANNNNTTC 1 cut(s) 631
Asp718I GGTACC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 2 cut(s) 276, 1015
AsuNHI GCTAGC 1 cut(s) 416
BamHI GGATCC 1 cut(s) 921
BanI GGYRCC 2 cut(s) 275, 590
BarI GAAGNNNNNNTAC 2 cut(s) 863, 895
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 5 cut(s) 91, 307, 356, 551, 1259
BccI CCATC 3 cut(s) 131, 763, 994
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 2 cut(s) 97, 353
BcoDI GTCTC 1 cut(s) 361
BcuI ACTAGT 1 cut(s) 712
BfaI CTAG 3 cut(s) 417, 713, 936
BfmI CTRYAG 1 cut(s) 764
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 5 cut(s) 105, 321, 345, 565, 1273
BlsI GCNGC 5 cut(s) 106, 322, 346, 566, 1274
Bme1390I CCNGG 2 cut(s) 97, 353
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 5 cut(s) 277, 592, 795, 923, 1290
BmrFI CCNGG 2 cut(s) 97, 353
BmsI GCATC 2 cut(s) 91, 551
BmtI GCTAGC 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 396, 428
BpmI CTGGAG 1 cut(s) 909
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 964
BsaBI GATNNNNATC 1 cut(s) 1041
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 4 cut(s) 95, 96, 351, 647
BsaWI WCCGGW 1 cut(s) 1007
Bse1I ACTGG 2 cut(s) 662, 862
Bse3DI GCAATG 1 cut(s) 290
Bse8I GATNNNNATC 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 97, 353
BseDI CCNNGG 4 cut(s) 95, 96, 351, 647
BseGI GGATG 2 cut(s) 106, 566
BseJI GATNNNNATC 1 cut(s) 1041
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 443, 897
BseNI ACTGG 2 cut(s) 662, 862
BseXI GCAGC 5 cut(s) 91, 307, 356, 551, 1259
BsgI GTGCAG 2 cut(s) 225, 936
BshFI GGCC 2 cut(s) 18, 875
BshNI GGYRCC 2 cut(s) 275, 590
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 2 cut(s) 1008, 1211
BsmAI GTCTC 1 cut(s) 361
BsmI GAATGC 2 cut(s) 433, 743
BsnI GGCC 2 cut(s) 18, 875
Bso31I GGTCTC 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 414
Bsp1407I TGTACA 2 cut(s) 619, 957
Bsp143I GATC 5 cut(s) 760, 801, 921, 1042, 1070
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 3 cut(s) 464, 529, 861
BspANI GGCC 2 cut(s) 18, 875
BspCNI CTCAG 2 cut(s) 444, 896
BspLI GGNNCC 5 cut(s) 277, 592, 795, 923, 1290
BspMAI CTGCAG 1 cut(s) 768
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 3 cut(s) 916, 929, 1078
BspT107I GGYRCC 2 cut(s) 275, 590
BspTNI GGTCTC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 290
BsrGI TGTACA 2 cut(s) 619, 957
BsrI ACTGG 2 cut(s) 662, 862
BssECI CCNNGG 4 cut(s) 95, 96, 351, 647
BssMI GATC 5 cut(s) 760, 801, 921, 1042, 1070
BssT1I CCWWGG 1 cut(s) 647
Bst2UI CCWGG 2 cut(s) 97, 353
Bst4CI ACNGT 3 cut(s) 112, 261, 301
Bst6I CTCTTC 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 740
BstAUI TGTACA 2 cut(s) 619, 957
BstC8I GCNNGC 8 cut(s) 160, 208, 414, 418, 1019, 1107, 1270, 1277
BstDEI CTNAG 2 cut(s) 452, 883
BstDSI CCRYGG 1 cut(s) 647
BstEII GGTNACC 1 cut(s) 1003
BstF5I GGATG 2 cut(s) 106, 566
BstKTI GATC 5 cut(s) 763, 804, 924, 1045, 1073
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 5 cut(s) 760, 801, 921, 1042, 1070
BstMWI GCNNNNNNNGC 2 cut(s) 740, 779
BstNI CCWGG 2 cut(s) 97, 353
BstNSI RCATGY 3 cut(s) 367, 509, 1021
BstPI GGTNACC 1 cut(s) 1003
BstSCI CCNGG 2 cut(s) 95, 351
BstSFI CTRYAG 1 cut(s) 764
BstV1I GCAGC 5 cut(s) 91, 307, 356, 551, 1259
BstX2I RGATCY 2 cut(s) 921, 1042
BstXI CCANNNNNNTGG 1 cut(s) 715
BstYI RGATCY 2 cut(s) 921, 1042
BsuRI GGCC 2 cut(s) 18, 875
BtgI CCRYGG 1 cut(s) 647
BtsCI GGATG 2 cut(s) 106, 566
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 3 cut(s) 36, 108, 1239
Cac8I GCNNGC 8 cut(s) 160, 208, 414, 418, 1019, 1107, 1270, 1277
CaiI CAGNNNCTG 1 cut(s) 1238
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 6 cut(s) 257, 276, 526, 620, 655, 958
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviAII CATG 8 cut(s) 200, 364, 506, 648, 955, 1018, 1098, 1169
CviQI GTAC 6 cut(s) 257, 276, 526, 620, 655, 958
DdeI CTNAG 2 cut(s) 452, 883
DpnI GATC 5 cut(s) 762, 803, 923, 1044, 1072
DpnII GATC 5 cut(s) 760, 801, 921, 1042, 1070
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
EciI GGCGGA 1 cut(s) 876
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 875
Eco31I GGTCTC 1 cut(s) 361
Eco32I GATATC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 1106
Eco91I GGTNACC 1 cut(s) 1003
EcoO65I GGTNACC 1 cut(s) 1003
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 95, 351
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 647
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 8 cut(s) 203, 367, 509, 651, 958, 1021, 1101, 1172
FalI AAGNNNNNCTT 2 cut(s) 619, 651
FatI CATG 8 cut(s) 199, 363, 505, 647, 954, 1017, 1097, 1168
FauNDI CATATG 1 cut(s) 21
FblI GTMKAC 1 cut(s) 1173
Fnu4HI GCNGC 5 cut(s) 105, 321, 345, 565, 1273
FokI GGATG 2 cut(s) 113, 573
Fsp4HI GCNGC 5 cut(s) 105, 321, 345, 565, 1273
FspBI CTAG 3 cut(s) 417, 713, 936
GluI GCNGC 5 cut(s) 105, 321, 345, 565, 1273
GsuI CTGGAG 1 cut(s) 909
HaeIII GGCC 2 cut(s) 18, 875
HapII CCGG 2 cut(s) 1008, 1211
Hin1II CATG 8 cut(s) 203, 367, 509, 651, 958, 1021, 1101, 1172
HindIII AAGCTT 1 cut(s) 940
HinfI GANTC 4 cut(s) 643, 864, 1139, 1191
HpaII CCGG 2 cut(s) 1008, 1211
HphI GGTGA 2 cut(s) 276, 1015
Hpy166II GTNNAC 4 cut(s) 54, 620, 1174, 1223
Hpy188I TCNGA 1 cut(s) 426
Hpy188III TCNNGA 4 cut(s) 73, 490, 664, 868
Hpy8I GTNNAC 4 cut(s) 54, 620, 1174, 1223
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 1 cut(s) 865
HpyCH4III ACNGT 3 cut(s) 112, 261, 301
HpyCH4IV ACGT 1 cut(s) 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 779
HpyF3I CTNAG 2 cut(s) 452, 883
HpySE526I ACGT 1 cut(s) 653
Hsp92II CATG 8 cut(s) 203, 367, 509, 651, 958, 1021, 1101, 1172
KpnI GGTACC 1 cut(s) 279
Kzo9I GATC 5 cut(s) 760, 801, 921, 1042, 1070
LmnI GCTCC 2 cut(s) 890, 1288
Lsp1109I GCAGC 5 cut(s) 91, 307, 356, 551, 1259
LweI GCATC 2 cut(s) 91, 551
MaeI CTAG 3 cut(s) 417, 713, 936
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 4 cut(s) 305, 1003, 1183, 1240
MalI GATC 5 cut(s) 762, 803, 923, 1044, 1072
MboI GATC 5 cut(s) 760, 801, 921, 1042, 1070
MboII GAAGA 5 cut(s) 228, 273, 544, 627, 1112
MflI RGATCY 2 cut(s) 921, 1042
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 6 cut(s) 191, 267, 376, 391, 631, 1032
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 4 cut(s) 289, 1268, 1290, 1302
MroXI GAANNNNTTC 1 cut(s) 631
MseI TTAA 5 cut(s) 330, 608, 720, 995, 1031
MslI CAYNNNNRTG 2 cut(s) 113, 368
MspA1I CMGCKG 2 cut(s) 531, 887
MspI CCGG 2 cut(s) 1008, 1211
MspR9I CCNGG 2 cut(s) 97, 353
Mva1269I GAATGC 2 cut(s) 433, 743
MvaI CCWGG 2 cut(s) 97, 353
MwoI GCNNNNNNNGC 2 cut(s) 740, 779
NcoI CCATGG 1 cut(s) 647
NdeI CATATG 1 cut(s) 21
NdeII GATC 5 cut(s) 760, 801, 921, 1042, 1070
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 8 cut(s) 203, 367, 509, 651, 958, 1021, 1101, 1172
NlaIV GGNNCC 5 cut(s) 277, 592, 795, 923, 1290
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 1003
NspI RCATGY 3 cut(s) 367, 509, 1021
PaeI GCATGC 1 cut(s) 1021
PasI CCCWGGG 1 cut(s) 96
PceI AGGCCT 1 cut(s) 875
PciI ACATGT 1 cut(s) 363
PctI GAATGC 2 cut(s) 433, 743
PdmI GAANNNNTTC 1 cut(s) 631
PfeI GAWTC 4 cut(s) 643, 864, 1139, 1191
PkrI GCNGC 5 cut(s) 106, 322, 346, 566, 1274
PscI ACATGT 1 cut(s) 363
PsiI TTATAA 1 cut(s) 476
Psp6I CCWGG 2 cut(s) 95, 351
PspEI GGTNACC 1 cut(s) 1003
PspGI CCWGG 2 cut(s) 95, 351
PspN4I GGNNCC 5 cut(s) 277, 592, 795, 923, 1290
PspPI GGNCC 1 cut(s) 16
PstI CTGCAG 1 cut(s) 768
PstNI CAGNNNCTG 1 cut(s) 1238
PsuI RGATCY 2 cut(s) 921, 1042
PvuII CAGCTG 1 cut(s) 887
RsaI GTAC 6 cut(s) 258, 277, 527, 621, 656, 959
RsaNI GTAC 6 cut(s) 257, 276, 526, 620, 655, 958
RseI CAYNNNNRTG 2 cut(s) 113, 368
SaqAI TTAA 5 cut(s) 330, 608, 720, 995, 1031
SatI GCNGC 5 cut(s) 105, 321, 345, 565, 1273
Sau3AI GATC 5 cut(s) 760, 801, 921, 1042, 1070
Sau96I GGNCC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 97, 353
SduI GDGCHC 1 cut(s) 414
SfaNI GCATC 2 cut(s) 91, 551
SfcI CTRYAG 1 cut(s) 764
SmiMI CAYNNNNRTG 2 cut(s) 113, 368
SmlI CTYRAG 1 cut(s) 943
SmoI CTYRAG 1 cut(s) 943
SpeI ACTAGT 1 cut(s) 712
SphI GCATGC 1 cut(s) 1021
Sse9I AATT 6 cut(s) 191, 267, 376, 391, 631, 1032
SseBI AGGCCT 1 cut(s) 875
SsiI CCGC 3 cut(s) 464, 529, 861
SspMI CTAG 3 cut(s) 417, 713, 936
StuI AGGCCT 1 cut(s) 875
StyD4I CCNGG 2 cut(s) 95, 351
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 3 cut(s) 112, 261, 301
TaiI ACGT 1 cut(s) 656
TaqI TCGA 1 cut(s) 1250
TasI AATT 6 cut(s) 191, 267, 376, 391, 631, 1032
TatI WGTACW 2 cut(s) 619, 957
TfiI GAWTC 4 cut(s) 643, 864, 1139, 1191
Tru1I TTAA 5 cut(s) 330, 608, 720, 995, 1031
Tru9I TTAA 5 cut(s) 330, 608, 720, 995, 1031
TscAI CASTG 3 cut(s) 43, 115, 1239
TseFI GTSAC 1 cut(s) 1003
TseI GCWGC 5 cut(s) 104, 320, 344, 564, 1272
Tsp45I GTSAC 1 cut(s) 1003
TspDTI ATGAA 4 cut(s) 219, 544, 943, 1268
TspRI CASTG 3 cut(s) 43, 115, 1239
XapI RAATTY 2 cut(s) 267, 391
XceI RCATGY 3 cut(s) 367, 509, 1021
XcmI CCANNNNNNNNNTGG 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 1173
XmnI GAANNNNTTC 1 cut(s) 631
XspI CTAG 3 cut(s) 417, 713, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.