AT3G62290

ADP-ribosylation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006984
Physical Location & Seq
Forward (+)
23051544 .. 23053893
2350 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62290.2

Sequence Viewer

Length: 546 bp
ATGGGTCTATCCTTCGGAAAGTTGTTCAGCAAGCTCTTTGCAAAGAAAGAGATGCGTATCCTCATGGTGGGTCTCGATGCAGCTGGTAAGACTACGATCTTGTACAAGCTCAAGCTTGGAGAGATCGTGACTACGATTCCAACCATTGGTTTCAATGTGGAGACTGTGGAATACAAGAACATTAGCTTTACCGTGTGGGATGTCGGGGGTCAAGACAAGATTCGTCCATTGTGGAGGCACTACTTCCAGAACACTCAGGGACTTATCTTTGTGGTGGACAGCAATGATCGTGACCGTGTTGTGGAAGCCAGAGATGAGCTTCACAGAATGTTGAACGAAGATGAGTTGAGAGATGCTGTGCTTCTCGTTTTTGCTAACAAGCAAGATCTTCCAAATGCGATGAACGCCGCTGAGATAACCGATAAGCTTGGACTTCACTCTCTCCGTCAACGACACTGGTACATACAGAGCACATGTGCTACCTCCGGAGAAGGGCTTTATGAGGGACTTGACTGGCTCTCTAACAACATCGCAAACAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

181

Amino Acids

20.61

Weight (kDa)

6.43

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 6 - 177 8.1e-82 ADP-ribosylation factor family
SRPRB PF09439 16 - 143 4.8e-13 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 8.3e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 173 5e-12 Ras family
Gtr1_RagA PF04670 19 - 141 1.6e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 45 - 111 8.1e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 146
AccIII TCCGGA 1 cut(s) 485
AciI CCGC 1 cut(s) 408
AfaI GTAC 2 cut(s) 104, 461
AfiI CCNNNNNNNGG 4 cut(s) 67, 146, 301, 492
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 2 cut(s) 154, 334
AluBI AGCT 7 cut(s) 34, 83, 109, 115, 186, 319, 427
AluI AGCT 7 cut(s) 34, 83, 109, 115, 186, 319, 427
Alw21I GWGCWC 1 cut(s) 473
Alw26I GTCTC 2 cut(s) 77, 155
Aor13HI TCCGGA 1 cut(s) 485
ApeKI GCWGC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 473
BbvI GCAGC 1 cut(s) 92
BciVI GTATCC 1 cut(s) 68
BcoDI GTCTC 2 cut(s) 77, 155
BfuI GTATCC 1 cut(s) 68
BglII AGATCT 1 cut(s) 385
BisI GCNGC 2 cut(s) 81, 408
BlsI GCNGC 2 cut(s) 82, 409
BmsI GCATC 3 cut(s) 42, 67, 343
BpuEI CTTGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 146, 301, 492
Bse1I ACTGG 2 cut(s) 461, 518
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 56
BseAI TCCGGA 1 cut(s) 485
BseGI GGATG 1 cut(s) 205
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 4 cut(s) 67, 146, 301, 492
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 269, 402
BseNI ACTGG 2 cut(s) 461, 518
BseXI GCAGC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 473
BsiSI CCGG 1 cut(s) 486
BslFI GGGAC 2 cut(s) 273, 519
BslI CCNNNNNNNGG 4 cut(s) 67, 146, 301, 492
BsmAI GTCTC 2 cut(s) 77, 155
BsmFI GGGAC 2 cut(s) 273, 519
Bso31I GGTCTC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 473
Bsp13I TCCGGA 1 cut(s) 485
Bsp1407I TGTACA 1 cut(s) 102
Bsp143I GATC 4 cut(s) 96, 123, 286, 385
BspACI CCGC 1 cut(s) 408
BspCNI CTCAG 2 cut(s) 268, 403
BspEI TCCGGA 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 289
BsrGI TGTACA 1 cut(s) 102
BsrI ACTGG 2 cut(s) 461, 518
BssMI GATC 4 cut(s) 96, 123, 286, 385
Bst4CI ACNGT 3 cut(s) 166, 193, 296
BstAUI TGTACA 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 3 cut(s) 255, 411, 543
BstF5I GGATG 1 cut(s) 205
BstKTI GATC 4 cut(s) 99, 126, 289, 388
BstMAI GTCTC 2 cut(s) 77, 155
BstMBI GATC 4 cut(s) 96, 123, 286, 385
BstMWI GCNNNNNNNGC 2 cut(s) 404, 539
BstNSI RCATGY 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuI GTATCC 1 cut(s) 68
BtgZI GCGATG 2 cut(s) 413, 514
BtsCI GGATG 1 cut(s) 205
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 1 cut(s) 32
Csp6I GTAC 2 cut(s) 103, 460
CviAII CATG 2 cut(s) 64, 474
CviQI GTAC 2 cut(s) 103, 460
DdeI CTNAG 3 cut(s) 255, 411, 543
DpnI GATC 4 cut(s) 98, 125, 288, 387
DpnII GATC 4 cut(s) 96, 123, 286, 385
Eco31I GGTCTC 1 cut(s) 77
FaeI CATG 2 cut(s) 67, 477
FaiI YATR 4 cut(s) 65, 464, 475, 501
FaqI GGGAC 2 cut(s) 273, 519
FatI CATG 2 cut(s) 63, 473
Fnu4HI GCNGC 2 cut(s) 81, 408
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 2 cut(s) 81, 408
GluI GCNGC 2 cut(s) 81, 408
HapII CCGG 1 cut(s) 486
Hin1II CATG 2 cut(s) 67, 477
HincII GTYRAC 1 cut(s) 449
HindII GTYRAC 1 cut(s) 449
HindIII AAGCTT 2 cut(s) 113, 425
HinfI GANTC 2 cut(s) 136, 220
HpaII CCGG 1 cut(s) 486
Hpy166II GTNNAC 2 cut(s) 277, 449
Hpy188I TCNGA 1 cut(s) 17
Hpy188III TCNNGA 6 cut(s) 74, 127, 212, 247, 290, 486
Hpy8I GTNNAC 2 cut(s) 277, 449
HpyAV CCTTC 2 cut(s) 22, 485
HpyCH4III ACNGT 3 cut(s) 166, 193, 296
HpyCH4V TGCA 2 cut(s) 41, 80
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 539
HpyF3I CTNAG 3 cut(s) 255, 411, 543
Hsp92II CATG 2 cut(s) 67, 477
Kpn2I TCCGGA 1 cut(s) 485
Kzo9I GATC 4 cut(s) 96, 123, 286, 385
LpnPI CCDG 7 cut(s) 69, 242, 260, 322, 442, 499, 499
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 3 cut(s) 42, 67, 343
MaeIII GTNAC 2 cut(s) 127, 290
MalI GATC 4 cut(s) 98, 125, 288, 387
MboI GATC 4 cut(s) 96, 123, 286, 385
MboII GAAGA 2 cut(s) 350, 380
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 164
MnlI CCTC 4 cut(s) 71, 228, 493, 496
MroI TCCGGA 1 cut(s) 485
MspA1I CMGCKG 2 cut(s) 83, 410
MspI CCGG 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 404, 539
NdeII GATC 4 cut(s) 96, 123, 286, 385
NlaIII CATG 2 cut(s) 67, 477
NmuCI GTSAC 2 cut(s) 127, 290
NspI RCATGY 1 cut(s) 477
PciI ACATGT 1 cut(s) 473
PfeI GAWTC 2 cut(s) 136, 220
PflMI CCANNNNNTGG 1 cut(s) 146
PkrI GCNGC 2 cut(s) 82, 409
PscI ACATGT 1 cut(s) 473
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 2 cut(s) 104, 461
RsaNI GTAC 2 cut(s) 103, 460
SatI GCNGC 2 cut(s) 81, 408
Sau3AI GATC 4 cut(s) 96, 123, 286, 385
SduI GDGCHC 1 cut(s) 473
SetI ASST 8 cut(s) 36, 85, 111, 117, 188, 321, 429, 485
SfaNI GCATC 3 cut(s) 42, 67, 343
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SsiI CCGC 1 cut(s) 408
TaaI ACNGT 3 cut(s) 166, 193, 296
TaqI TCGA 1 cut(s) 75
TatI WGTACW 1 cut(s) 102
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 2 cut(s) 136, 220
TscAI CASTG 1 cut(s) 461
TseFI GTSAC 2 cut(s) 127, 290
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 2 cut(s) 127, 290
TspDTI ATGAA 1 cut(s) 416
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 1 cut(s) 461
Van91I CCANNNNNTGG 1 cut(s) 146
XceI RCATGY 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.