Rw4G023330
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006984
Physical Location & Seq
Forward (+)
48937700 .. 48941166
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G023330.1

Sequence Viewer

Length: 690 bp
ATGGCGCGAGGGACGAAGAGAACTGCCTCTCCGGAATCCGAATCTGAATCTGCATCTGCATCTGCTTTTCAGATTAAGAAAGTAGTGACCGTACGGCCCCATAAGACCCACACATGCACACCTCTCTTTCTACCATACAAGATGGGGTTGTCTTTCTCCAAACTTTTTAGCAAACTTTTTGCTAAGAAAGATATGCGTATTCTTATGGTGGGTCTTGATGCTGCTGGTAAAACCACCATTTTGTATAAGCTCAAGTTAGGCGAAATCGTTACCACCATTCCTACCATTGGATTTAATGTGGAAACTGTGGAATACAAAAACATCAGCTTCACAGTGTGGGATGTTGGGGGCCAAGACAAGATTCGACCACTATGGAGACACTACTTCCAAAACACACAAGGGCTTGTCTTTGTGATTGACAGCAATGATCGAGATCGTGTTGTTGAGGCTAAAGATGAGCTGCACAGGATGTTGAATGAGGACGAGTTGAGGGATGCTGTGCTTCTTGTGTTTGCAAACAAGCAAGATCTTCCAAATGCAATGAATGCTGCTGAGATCACCGATAAGCTTGGCCTTCATTCTCTTCGACAACGCCATTGGTATATCCAGAGTACATGTGCCACTTCCGGTGAAGGATTGTACGAGGGATTGGACTGGCTTTCAAACAACATTGCTAACAAGCAAACTTGA

Protein Analysis

229

Amino Acids

25.91

Weight (kDa)

8.97

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 52 - 224 3.4e-81 ADP-ribosylation factor family
SRPRB PF09439 64 - 190 1.8e-12 Signal recognition particle receptor beta subunit
Roc PF08477 66 - 176 2.6e-13 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 66 - 220 1.4e-11 Ras family
Gtr1_RagA PF04670 66 - 187 2.1e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 91 - 159 3.9e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 31
AdeI CACNNNGTG 1 cut(s) 336
AfaI GTAC 3 cut(s) 93, 613, 641
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 614
AgsI TTSAA 2 cut(s) 475, 663
AjuI GAANNNNNNNTTGG 2 cut(s) 345, 377
AloI GAACNNNNNNTCC 2 cut(s) 13, 45
AluBI AGCT 4 cut(s) 250, 327, 460, 568
AluI AGCT 4 cut(s) 250, 327, 460, 568
Alw26I GTCTC 1 cut(s) 370
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 3 cut(s) 95, 349, 571
ApeKI GCWGC 3 cut(s) 221, 460, 548
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 96, 349
AsuHPI GGTGA 2 cut(s) 550, 641
BbvI GCAGC 3 cut(s) 208, 447, 535
BccI CCATC 1 cut(s) 136
BceAI ACGGC 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 370
BglII AGATCT 1 cut(s) 526
BisI GCNGC 3 cut(s) 222, 461, 549
BlsI GCNGC 3 cut(s) 223, 462, 550
BmgT120I GGNCC 2 cut(s) 96, 349
BmiI GGNNCC 2 cut(s) 98, 350
BmsI GCATC 4 cut(s) 62, 68, 208, 484
BpuEI CTTGAG 1 cut(s) 236
BsaBI GATNNNNATC 1 cut(s) 432
BsaWI WCCGGW 2 cut(s) 31, 626
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 659
Bse3DI GCAATG 3 cut(s) 430, 546, 669
Bse8I GATNNNNATC 1 cut(s) 432
BseAI TCCGGA 1 cut(s) 31
BseGI GGATG 3 cut(s) 346, 474, 499
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 3 cut(s) 430, 546, 669
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 1 cut(s) 659
BseXI GCAGC 3 cut(s) 208, 447, 535
BsgI GTGCAG 1 cut(s) 446
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 3 cut(s) 97, 351, 573
BsiSI CCGG 2 cut(s) 32, 627
BsiWI CGTACG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 25
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 3 cut(s) 97, 351, 573
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 4 cut(s) 427, 433, 526, 555
BspANI GGCC 3 cut(s) 97, 351, 573
BspCNI CTCAG 1 cut(s) 544
BspEI TCCGGA 1 cut(s) 31
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 98, 350
BsrDI GCAATG 3 cut(s) 430, 546, 669
BsrI ACTGG 1 cut(s) 659
BssMI GATC 4 cut(s) 427, 433, 526, 555
Bst4CI ACNGT 3 cut(s) 91, 307, 334
Bst6I CTCTTC 2 cut(s) 11, 588
BstAPI GCANNNNNTGC 1 cut(s) 545
BstDEI CTNAG 2 cut(s) 183, 552
BstF5I GGATG 3 cut(s) 346, 474, 499
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 430, 436, 529, 558
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 4 cut(s) 427, 433, 526, 555
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNSI RCATGY 2 cut(s) 117, 618
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 3 cut(s) 208, 447, 535
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 3 cut(s) 97, 351, 573
BtsCI GGATG 3 cut(s) 346, 474, 499
BtsIMutI CAGTG 1 cut(s) 339
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 96, 349
Csp6I GTAC 3 cut(s) 92, 612, 640
CviAII CATG 2 cut(s) 114, 615
CviQI GTAC 3 cut(s) 92, 612, 640
DdeI CTNAG 2 cut(s) 183, 552
DpnI GATC 4 cut(s) 429, 435, 528, 557
DpnII GATC 4 cut(s) 427, 433, 526, 555
DraIII CACNNNGTG 1 cut(s) 336
Eam1104I CTCTTC 2 cut(s) 11, 588
EarI CTCTTC 2 cut(s) 11, 588
FaeI CATG 2 cut(s) 117, 618
FaiI YATR 9 cut(s) 102, 115, 136, 194, 206, 246, 373, 603, 616
FaqI GGGAC 1 cut(s) 25
FatI CATG 2 cut(s) 113, 614
Fnu4HI GCNGC 3 cut(s) 222, 461, 549
FokI GGATG 3 cut(s) 353, 481, 506
Fsp4HI GCNGC 3 cut(s) 222, 461, 549
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 222, 461, 549
HaeIII GGCC 3 cut(s) 97, 351, 573
HapII CCGG 2 cut(s) 32, 627
HhaI GCGC 1 cut(s) 7
Hin1II CATG 2 cut(s) 117, 618
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 4 cut(s) 35, 41, 47, 361
HpaII CCGG 2 cut(s) 32, 627
HphI GGTGA 2 cut(s) 550, 641
Hpy188I TCNGA 3 cut(s) 40, 46, 72
Hpy188III TCNNGA 4 cut(s) 32, 215, 431, 607
HpyAV CCTTC 2 cut(s) 584, 626
HpyCH4III ACNGT 3 cut(s) 91, 307, 334
HpyCH4V TGCA 6 cut(s) 53, 59, 117, 463, 515, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 2 cut(s) 183, 552
Hsp92II CATG 2 cut(s) 117, 618
HspAI GCGC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 31
Kzo9I GATC 4 cut(s) 427, 433, 526, 555
LpnPI CCDG 6 cut(s) 45, 210, 451, 620, 640, 640
Lsp1109I GCAGC 3 cut(s) 208, 447, 535
LweI GCATC 4 cut(s) 62, 68, 208, 484
MaeIII GTNAC 2 cut(s) 85, 268
MalI GATC 4 cut(s) 429, 435, 528, 557
MboI GATC 4 cut(s) 427, 433, 526, 555
MboII GAAGA 3 cut(s) 28, 521, 575
MflI RGATCY 1 cut(s) 526
MnlI CCTC 6 cut(s) 37, 132, 439, 472, 483, 637
MroI TCCGGA 1 cut(s) 31
MseI TTAA 2 cut(s) 75, 294
MspI CCGG 2 cut(s) 32, 627
Mva1269I GAATGC 1 cut(s) 550
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeII GATC 4 cut(s) 427, 433, 526, 555
NlaIII CATG 2 cut(s) 117, 618
NlaIV GGNNCC 2 cut(s) 98, 350
NmuCI GTSAC 1 cut(s) 85
NspI RCATGY 2 cut(s) 117, 618
PciI ACATGT 1 cut(s) 614
PctI GAATGC 1 cut(s) 550
PfeI GAWTC 4 cut(s) 35, 41, 47, 361
Pfl23II CGTACG 1 cut(s) 91
PkrI GCNGC 3 cut(s) 223, 462, 550
PscI ACATGT 1 cut(s) 614
PspLI CGTACG 1 cut(s) 91
PspN4I GGNNCC 2 cut(s) 98, 350
PspPI GGNCC 2 cut(s) 96, 349
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 3 cut(s) 93, 613, 641
RsaNI GTAC 3 cut(s) 92, 612, 640
SaqAI TTAA 2 cut(s) 75, 294
SatI GCNGC 3 cut(s) 222, 461, 549
Sau3AI GATC 4 cut(s) 427, 433, 526, 555
Sau96I GGNCC 2 cut(s) 96, 349
SetI ASST 5 cut(s) 124, 252, 329, 462, 570
SfaNI GCATC 4 cut(s) 62, 68, 208, 484
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
TaaI ACNGT 3 cut(s) 91, 307, 334
TaqI TCGA 3 cut(s) 364, 430, 586
TatI WGTACW 1 cut(s) 611
TfiI GAWTC 4 cut(s) 35, 41, 47, 361
Tru1I TTAA 2 cut(s) 75, 294
Tru9I TTAA 2 cut(s) 75, 294
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 3 cut(s) 221, 460, 548
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 557, 566
TspRI CASTG 1 cut(s) 339
XceI RCATGY 2 cut(s) 117, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.