AT4G00335

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003342
Physical Location & Seq
Forward (+)
145887 .. 148424
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00335.1

Sequence Viewer

Length: 573 bp
ATGGGAGGTTGCTGTAGTTCTTCGAGGAAGTCACATCTTGTTGGAACACCTGTTTACTATTATTGTCCAGAATCTTTTGAAGAGCTTGTCCCTTCAGGAACCCGTGCTGGTGTGGGATCAGCATTCACCACAGGCCTCTTGGTTGATATAGGTTTGGAGACATCTATACCTGACACCTTTTGTGCTCCTGCTCCTCTTCCATATGATTTGCTTTTGGGGCGACCACAATGCACAGATTCTGAGTCCATCAAAGGAAGGATGAGTGGAAGCAGTTTTGAAACGCTAGCGACGTGTGAAGATCTTGGGGAATCAGACTGTAAAACTCTAGCCAGCTCAGTCATTCTTTCACCGAGGAAATCAGATTTCTCGAAACATCAAGGATTGAAGATATTGGTTGATGAAGAGGAGGACTGTTGTCCTATTTGCTTTGAAGATTATGATGTTGAGAATCCAAGACTAACGACAAAGTGTGAGCACGAATTTCACCTCTCTTGTCTTCTGGAGTGGATTGAAAGAAGCGATAGATGCCCAATATGTGATAAGGAAGTTGTGTTTGATGATCGCTTGAACTAG

Protein Analysis

190

Amino Acids

20.95

Weight (kDa)

4.52

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 135 - 179 6.4e-10 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 137 - 185 6.1e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 138 - 179 1.5e-10 Ring finger domain
zf-C3HC4_2 PF13923 139 - 179 5.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 139 - 179 1.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 139 - 177 3.8e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 124
AcsI RAATTY 1 cut(s) 479
AcuI CTGAAG 1 cut(s) 78
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 6 cut(s) 80, 278, 385, 431, 512, 568
AjiI CACGTC 1 cut(s) 291
AluBI AGCT 2 cut(s) 85, 333
AluI AGCT 2 cut(s) 85, 333
Alw21I GWGCWC 2 cut(s) 187, 477
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 124
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 1 cut(s) 133
ApoI RAATTY 1 cut(s) 479
AsuHPI GGTGA 3 cut(s) 118, 339, 476
AsuNHI GCTAGC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 488
Bbv12I GWGCWC 2 cut(s) 187, 477
BccI CCATC 1 cut(s) 254
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 3 cut(s) 284, 326, 571
BfmI CTRYAG 1 cut(s) 13
BglII AGATCT 1 cut(s) 298
BmgBI CACGTC 1 cut(s) 291
BmiI GGNNCC 1 cut(s) 100
BmsI GCATC 1 cut(s) 515
BmtI GCTAGC 1 cut(s) 287
BoxI GACNNNNGTC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 521
BsaJI CCNNGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 350
BseGI GGATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 231, 348
BseRI GAGGAG 2 cut(s) 183, 419
BshFI GGCC 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 187, 477
BslFI GGGAC 1 cut(s) 74
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 187, 477
Bsp143I GATC 3 cut(s) 116, 298, 559
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 2 cut(s) 232, 347
BspLI GGNNCC 1 cut(s) 100
BspOI GCTAGC 1 cut(s) 287
BspPI GGATC 1 cut(s) 124
BspQI GCTCTTC 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 350
BssMI GATC 3 cut(s) 116, 298, 559
Bst4CI ACNGT 2 cut(s) 317, 413
Bst6I CTCTTC 3 cut(s) 75, 201, 396
BstC8I GCNNGC 2 cut(s) 285, 331
BstDEI CTNAG 2 cut(s) 240, 334
BstF5I GGATG 1 cut(s) 264
BstKTI GATC 3 cut(s) 119, 301, 562
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 116, 298, 559
BstMWI GCNNNNNNNGC 2 cut(s) 217, 525
BstPAI GACNNNNGTC 1 cut(s) 414
BstSFI CTRYAG 1 cut(s) 13
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 1 cut(s) 135
BtrI CACGTC 1 cut(s) 291
BtsCI GGATG 1 cut(s) 264
Cac8I GCNNGC 2 cut(s) 285, 331
CaiI CAGNNNCTG 1 cut(s) 239
CviJI RGCY 4 cut(s) 85, 135, 329, 333
CviKI_1 RGCY 4 cut(s) 85, 135, 329, 333
DdeI CTNAG 2 cut(s) 240, 334
DpnI GATC 3 cut(s) 118, 300, 561
DpnII GATC 3 cut(s) 116, 298, 559
Eam1104I CTCTTC 3 cut(s) 75, 201, 396
EarI CTCTTC 3 cut(s) 75, 201, 396
Eco147I AGGCCT 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 78
FaiI YATR 6 cut(s) 149, 167, 202, 204, 438, 535
FaqI GGGAC 1 cut(s) 74
FauNDI CATATG 1 cut(s) 202
FokI GGATG 1 cut(s) 271
FspBI CTAG 3 cut(s) 284, 326, 571
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 1 cut(s) 135
HinfI GANTC 5 cut(s) 71, 236, 242, 308, 448
HphI GGTGA 3 cut(s) 118, 339, 476
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 241, 313, 361
Hpy188III TCNNGA 4 cut(s) 68, 96, 367, 500
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 2 cut(s) 102, 249
HpyCH4III ACNGT 2 cut(s) 317, 413
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 525
HpyF3I CTNAG 2 cut(s) 240, 334
HpySE526I ACGT 1 cut(s) 290
Kzo9I GATC 3 cut(s) 116, 298, 559
LguI GCTCTTC 1 cut(s) 75
LmnI GCTCC 2 cut(s) 190, 196
LpnPI CCDG 9 cut(s) 63, 81, 81, 93, 117, 183, 201, 343, 485
LweI GCATC 1 cut(s) 515
MaeI CTAG 3 cut(s) 284, 326, 571
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 1 cut(s) 30
MalI GATC 3 cut(s) 118, 300, 561
MboI GATC 3 cut(s) 116, 298, 559
MboII GAAGA 8 cut(s) 12, 92, 188, 308, 397, 413, 443, 488
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 2 cut(s) 187, 477
MluCI AATT 1 cut(s) 479
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 22
MnlI CCTC 7 cut(s) 18, 146, 204, 345, 397, 400, 497
Mva1269I GAATGC 1 cut(s) 122
MwoI GCNNNNNNNGC 2 cut(s) 217, 525
NdeI CATATG 1 cut(s) 202
NdeII GATC 3 cut(s) 116, 298, 559
NheI GCTAGC 1 cut(s) 283
NlaIV GGNNCC 1 cut(s) 100
NmuCI GTSAC 1 cut(s) 30
PceI AGGCCT 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 4 cut(s) 71, 236, 308, 448
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PshAI GACNNNNGTC 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 100
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 298
SapI GCTCTTC 1 cut(s) 75
Sau3AI GATC 3 cut(s) 116, 298, 559
SchI GAGTC 1 cut(s) 251
SduI GDGCHC 2 cut(s) 187, 477
SetI ASST 9 cut(s) 10, 52, 87, 154, 172, 179, 293, 335, 489
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 13
Sse9I AATT 1 cut(s) 479
SseBI AGGCCT 1 cut(s) 135
SspMI CTAG 3 cut(s) 284, 326, 571
StuI AGGCCT 1 cut(s) 135
TaaI ACNGT 2 cut(s) 317, 413
TaiI ACGT 1 cut(s) 293
TaqI TCGA 2 cut(s) 23, 368
TasI AATT 1 cut(s) 479
TfiI GAWTC 4 cut(s) 71, 236, 308, 448
TseFI GTSAC 1 cut(s) 30
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 1 cut(s) 414
XapI RAATTY 1 cut(s) 479
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XspI CTAG 3 cut(s) 284, 326, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.