Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005829

Physical Location & Seq

Genomic Coordinates Reverse (-)

211000 .. 213697

Gene Structure 2698 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G00460.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1014 bp

ATGGAGAATTTATCGAATCCAGATGAAAACGACGATCATCAATCACCTAGATCCATTGACCAAAATGATCAATCAGCAGTAGAAACTCCGGTTTACTCGACGATGAGCATAGATTCCTTTGTTTACCCTCGAACTTGTTCAGAGACCACTTCAGGGTTTTCAGACCAGATAGATGAAACCAACAGCTTCTGTAGTGAAGCTTCTCCTTGTAACTGGCCTGTCCTTACTGAATCTAAGAGCTCCAAATGTCTCTCTGGTTTAGAGATGCAATCAAATGAATGTCTTGTAGTCCAAGAAATTTCTGAACCAGAACTTGAGACAATGAAGGAAAGATTCGCTAAGCTTCTACTTGGAGAAGATATGTCAGGGAGTGGTAAAGGAGTTTGCACTGCAGTGACTATCTCAAACGCAATTACCAATCTTTATGCTACAGTGTTTGGACAGAATCTGAGGTTAGAGCCGTTAGAAACAGAGAAGAGAGCATTGTGGAAGAGAGAAATGAATTGTCTTTTATCTGTATGCGATTACATTGTCGAATTCATCCCTAGATGTCAGAATCTAAGCAATGGAGCTACTGTTGAGGTGATGGAGAGTAGACCAAGAGCAGATATCTATATCAACTTGCCTGCATTGAGAAAGCTAGATTCAATGCTTATGGAAGCATTGGATAGTTTTCAGAACACAGAGTTTTGGTATGCAGAAGAAGGAAGTTTATCGATGAAATCAGCTCGTTCTTCGACTGGATCATTCAGGAAAGTTATAGTACAAAGGAAAGAAGAGAAATGGTGGTTACCAGTTCCTCTAGTACCATCAGAAGGTTTGTCAGATAAAGCCAGAAAACAACTCAAGAACAAGAGAGAAAGTACCAATCAGATTCATAAAGCTGCAATGGCTATCAACAGTAGCATTCTCAGTGAAATGGAGATTCCAGACTCTTACATGACAACTCTTCCAAAGGTTTTTTTTTTTGTTACCTTCATTCATTCATACCTACTAGGAATTTTGAAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0009505 GO:0009531 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009664 GO:0009814 GO:0009817 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016043 GO:0016049 GO:0017016 GO:0017048 GO:0019899 GO:0021700 GO:0022622 GO:0030154 GO:0030312 GO:0031267 GO:0032501 GO:0032502 GO:0040007 GO:0043207 GO:0044424 GO:0044464 GO:0045087 GO:0045229 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051020 GO:0051704 GO:0051707 GO:0060560 GO:0065007 GO:0065009 GO:0071554 GO:0071555 GO:0071669 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0098542 GO:0098772 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PRONE

Protein Analysis

337

Amino Acids

38.05

Weight (kDa)

4.9

Isoelectric Point (pI)

52.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRONE	PF03759	104 - 470	6.7e-175	PRONE (Plant-specific Rop nucleotide exchanger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01700 AT1G01700 AT1G01700 AT1G01700 AT1G01700 AT4G00460 AT4G00460 AT4G00460 AT4G00460
fragaria_vesca	FvH4_7g14260 FvH4_7g14260 FvH4_7g14260 FvH4_7g14260
malus_domestica	MD01G1060500.v1.1 MD07G1137200.v1.1
prunus_persica	Prupe.2G170400_v2.0.a1
pyrus_communis	pycom07g13500
rosa_chinensis	RchiOBHm_Chr1g0355251
rosa_laevigata	RLG00000028167
rosa_multiflora	Rmu_sc0003115.1_g000043
rosa_roxburghii	Rroxscaffold_4G00300560
rosa_rugosa	Rorug01G0245800 Rorug01G0245800
rosa_samantha	Rh1AG258200 Rh1BG227800 Rh1CG241900 Rh1DG255600
rosa_wichuraiana	Rw1G022790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	595
AclWI	GGATC	2 cut(s)	45, 751
AcsI	RAATTY	4 cut(s)	7, 297, 536, 999
AcuI	CTGAAG	1 cut(s)	135
AfaI	GTAC	3 cut(s)	765, 807, 865
AfiI	CCNNNNNNNGG	2 cut(s)	153, 815
AgsI	TTSAA	2 cut(s)	648, 1006
AleI	CACNNNNGTG	1 cut(s)	392
AluBI	AGCT	8 cut(s)	186, 200, 240, 343, 572, 640, 728, 884
AluI	AGCT	8 cut(s)	186, 200, 240, 343, 572, 640, 728, 884
Alw21I	GWGCWC	1 cut(s)	242
Alw26I	GTCTC	3 cut(s)	137, 254, 311
AlwI	GGATC	2 cut(s)	45, 751
AlwNI	CAGNNNCTG	2 cut(s)	189, 448
AoxI	GGCC	1 cut(s)	215
ApeKI	GCWGC	1 cut(s)	884
ApoI	RAATTY	4 cut(s)	7, 297, 536, 999
Asp700I	GAANNNNTTC	1 cut(s)	136
AsuHPI	GGTGA	2 cut(s)	36, 595
BanII	GRGCYC	1 cut(s)	242
BarI	GAAGNNNNNNTAC	2 cut(s)	184, 216
Bbv12I	GWGCWC	1 cut(s)	242
BbvI	GCAGC	1 cut(s)	871
BccI	CCATC	2 cut(s)	580, 817
BceAI	ACGGC	1 cut(s)	445
BclI	TGATCA	1 cut(s)	67
BcoDI	GTCTC	3 cut(s)	137, 254, 311
BfaI	CTAG	5 cut(s)	48, 546, 641, 803, 995
BfmI	CTRYAG	3 cut(s)	190, 390, 429
BisI	GCNGC	1 cut(s)	885
BlpI	GCTNAGC	1 cut(s)	339
BlsI	GCNGC	1 cut(s)	886
BmsI	GCATC	1 cut(s)	255
Bpu1102I	GCTNAGC	1 cut(s)	339
BpuEI	CTTGAG	2 cut(s)	335, 830
Bsa29I	ATCGAT	1 cut(s)	716
BsaI	GGTCTC	1 cut(s)	137
BsaWI	WCCGGW	1 cut(s)	88
BsaXI	ACNNNNNCTCC	2 cut(s)	561, 591
Bsc4I	CCNNNNNNNGG	2 cut(s)	153, 815
Bse1I	ACTGG	3 cut(s)	218, 745, 794
Bse3DI	GCAATG	2 cut(s)	571, 894
BseCI	ATCGAT	1 cut(s)	716
BseGI	GGATG	1 cut(s)	540
BseLI	CCNNNNNNNGG	2 cut(s)	153, 815
BseMI	GCAATG	2 cut(s)	571, 894
BseMII	CTCAG	2 cut(s)	440, 925
BseNI	ACTGG	3 cut(s)	218, 745, 794
BseXI	GCAGC	1 cut(s)	871
BshFI	GGCC	1 cut(s)	217
BshVI	ATCGAT	1 cut(s)	716
BsiHKAI	GWGCWC	1 cut(s)	242
BsiSI	CCGG	1 cut(s)	89
BslI	CCNNNNNNNGG	2 cut(s)	153, 815
BsmAI	GTCTC	3 cut(s)	137, 254, 311
BsmI	GAATGC	1 cut(s)	906
BsnI	GGCC	1 cut(s)	217
Bso31I	GGTCTC	1 cut(s)	137
Bsp1286I	GDGCHC	1 cut(s)	242
Bsp143I	GATC	4 cut(s)	34, 50, 67, 743
Bsp1720I	GCTNAGC	1 cut(s)	339
BspANI	GGCC	1 cut(s)	217
BspCNI	CTCAG	2 cut(s)	441, 924
BspDI	ATCGAT	1 cut(s)	716
BspMAI	CTGCAG	1 cut(s)	394
BspPI	GGATC	2 cut(s)	45, 751
BspTNI	GGTCTC	1 cut(s)	137
BsrDI	GCAATG	2 cut(s)	571, 894
BsrI	ACTGG	3 cut(s)	218, 745, 794
BssMI	GATC	4 cut(s)	34, 50, 67, 743
Bst4CI	ACNGT	3 cut(s)	433, 577, 902
Bst6I	CTCTTC	4 cut(s)	470, 485, 771, 954
BstC8I	GCNNGC	1 cut(s)	627
BstDEI	CTNAG	5 cut(s)	234, 339, 449, 560, 911
BstEII	GGTNACC	1 cut(s)	789
BstF5I	GGATG	1 cut(s)	540
BstKTI	GATC	4 cut(s)	37, 53, 70, 746
BstMAI	GTCTC	3 cut(s)	137, 254, 311
BstMBI	GATC	4 cut(s)	34, 50, 67, 743
BstMWI	GCNNNNNNNGC	1 cut(s)	890
BstPI	GGTNACC	1 cut(s)	789
BstSFI	CTRYAG	3 cut(s)	190, 390, 429
BstV1I	GCAGC	1 cut(s)	871
BstX2I	RGATCY	1 cut(s)	50
BstYI	RGATCY	1 cut(s)	50
Bsu15I	ATCGAT	1 cut(s)	716
BsuRI	GGCC	1 cut(s)	217
BsuTUI	ATCGAT	1 cut(s)	716
BtsCI	GGATG	1 cut(s)	540
BtsI	GCAGTG	2 cut(s)	387, 399
BtsIMutI	CAGTG	4 cut(s)	387, 399, 438, 919
Cac8I	GCNNGC	1 cut(s)	627
CaiI	CAGNNNCTG	2 cut(s)	189, 448
ClaI	ATCGAT	1 cut(s)	716
Csp6I	GTAC	3 cut(s)	764, 806, 864
CviAII	CATG	1 cut(s)	940
CviQI	GTAC	3 cut(s)	764, 806, 864
DdeI	CTNAG	5 cut(s)	234, 339, 449, 560, 911
DpnI	GATC	4 cut(s)	36, 52, 69, 745
DpnII	GATC	4 cut(s)	34, 50, 67, 743
Eam1104I	CTCTTC	4 cut(s)	470, 485, 771, 954
EarI	CTCTTC	4 cut(s)	470, 485, 771, 954
Ecl136II	GAGCTC	1 cut(s)	240
Eco24I	GRGCYC	1 cut(s)	242
Eco31I	GGTCTC	1 cut(s)	137
Eco32I	GATATC	1 cut(s)	610
Eco53kI	GAGCTC	1 cut(s)	240
Eco57I	CTGAAG	1 cut(s)	135
Eco91I	GGTNACC	1 cut(s)	789
EcoICRI	GAGCTC	1 cut(s)	240
EcoO65I	GGTNACC	1 cut(s)	789
EcoRI	GAATTC	1 cut(s)	536
EcoRV	GATATC	1 cut(s)	610
EcoT38I	GRGCYC	1 cut(s)	242
FaeI	CATG	1 cut(s)	943
FatI	CATG	1 cut(s)	939
FbaI	TGATCA	1 cut(s)	67
FblI	GTMKAC	1 cut(s)	595
Fnu4HI	GCNGC	1 cut(s)	885
FokI	GGATG	1 cut(s)	527
FriOI	GRGCYC	1 cut(s)	242
Fsp4HI	GCNGC	1 cut(s)	885
FspBI	CTAG	5 cut(s)	48, 546, 641, 803, 995
GluI	GCNGC	1 cut(s)	885
HaeIII	GGCC	1 cut(s)	217
HapII	CCGG	1 cut(s)	89
Hin1II	CATG	1 cut(s)	943
HindIII	AAGCTT	2 cut(s)	198, 341
HpaII	CCGG	1 cut(s)	89
HphI	GGTGA	2 cut(s)	36, 595
Hpy166II	GTNNAC	3 cut(s)	94, 124, 596
Hpy188I	TCNGA	9 cut(s)	142, 163, 304, 450, 555, 678, 814, 826, 873
Hpy188III	TCNNGA	4 cut(s)	20, 751, 847, 929
Hpy8I	GTNNAC	3 cut(s)	94, 124, 596
Hpy99I	CGWCG	2 cut(s)	35, 103
HpyAV	CCTTC	4 cut(s)	319, 698, 809, 985
HpyCH4III	ACNGT	3 cut(s)	433, 577, 902
HpyCH4V	TGCA	6 cut(s)	268, 387, 392, 629, 698, 887
HpyF10VI	GCNNNNNNNGC	1 cut(s)	890
HpyF3I	CTNAG	5 cut(s)	234, 339, 449, 560, 911
Hsp92II	CATG	1 cut(s)	943
Ksp22I	TGATCA	1 cut(s)	67
Kzo9I	GATC	4 cut(s)	34, 50, 67, 743
LmnI	GCTCC	2 cut(s)	245, 569
Lsp1109I	GCAGC	1 cut(s)	871
LweI	GCATC	1 cut(s)	255
MaeI	CTAG	5 cut(s)	48, 546, 641, 803, 995
MaeIII	GTNAC	4 cut(s)	209, 394, 789, 970
MalI	GATC	4 cut(s)	36, 52, 69, 745
MboI	GATC	4 cut(s)	34, 50, 67, 743
MboII	GAAGA	7 cut(s)	368, 487, 502, 713, 726, 788, 941
MflI	RGATCY	1 cut(s)	50
MhlI	GDGCHC	1 cut(s)	242
MluCI	AATT	6 cut(s)	7, 297, 411, 502, 536, 999
MlyI	GAGTC	1 cut(s)	926
MnlI	CCTC	4 cut(s)	138, 444, 574, 810
MroXI	GAANNNNTTC	1 cut(s)	136
MslI	CAYNNNNRTG	1 cut(s)	392
MspI	CCGG	1 cut(s)	89
Mva1269I	GAATGC	1 cut(s)	906
MwoI	GCNNNNNNNGC	1 cut(s)	890
NdeII	GATC	4 cut(s)	34, 50, 67, 743
NlaIII	CATG	1 cut(s)	943
NmuCI	GTSAC	1 cut(s)	394
OliI	CACNNNNGTG	1 cut(s)	392
PctI	GAATGC	1 cut(s)	906
PdmI	GAANNNNTTC	1 cut(s)	136
PfeI	GAWTC	9 cut(s)	16, 113, 230, 333, 445, 556, 644, 874, 925
PkrI	GCNGC	1 cut(s)	886
PleI	GAGTC	1 cut(s)	926
PpsI	GAGTC	1 cut(s)	926
Psp124BI	GAGCTC	1 cut(s)	242
PspEI	GGTNACC	1 cut(s)	789
PstI	CTGCAG	1 cut(s)	394
PstNI	CAGNNNCTG	2 cut(s)	189, 448
PsuI	RGATCY	1 cut(s)	50
RsaI	GTAC	3 cut(s)	765, 807, 865
RsaNI	GTAC	3 cut(s)	764, 806, 864
RseI	CAYNNNNRTG	1 cut(s)	392
SacI	GAGCTC	1 cut(s)	242
SatI	GCNGC	1 cut(s)	885
Sau3AI	GATC	4 cut(s)	34, 50, 67, 743
SchI	GAGTC	1 cut(s)	926
SduI	GDGCHC	1 cut(s)	242
SfaNI	GCATC	1 cut(s)	255
SfcI	CTRYAG	3 cut(s)	190, 390, 429
SmiMI	CAYNNNNRTG	1 cut(s)	392
SmlI	CTYRAG	2 cut(s)	314, 845
SmoI	CTYRAG	2 cut(s)	314, 845
Sse9I	AATT	6 cut(s)	7, 297, 411, 502, 536, 999
SspMI	CTAG	5 cut(s)	48, 546, 641, 803, 995
SstI	GAGCTC	1 cut(s)	242
TaaI	ACNGT	3 cut(s)	433, 577, 902
TaqI	TCGA	6 cut(s)	14, 98, 130, 534, 716, 737
TasI	AATT	6 cut(s)	7, 297, 411, 502, 536, 999
TatI	WGTACW	1 cut(s)	763
TfiI	GAWTC	9 cut(s)	16, 113, 230, 333, 445, 556, 644, 874, 925
TscAI	CASTG	4 cut(s)	394, 399, 438, 919
TseFI	GTSAC	1 cut(s)	394
TseI	GCWGC	1 cut(s)	884
Tsp45I	GTSAC	1 cut(s)	394
TspRI	CASTG	4 cut(s)	394, 399, 438, 919
XapI	RAATTY	4 cut(s)	7, 297, 536, 999
XmiI	GTMKAC	1 cut(s)	595
XmnI	GAANNNNTTC	1 cut(s)	136
XspI	CTAG	5 cut(s)	48, 546, 641, 803, 995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.