Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005829

Physical Location & Seq

Genomic Coordinates Forward (+)

16409527 .. 16412784

Gene Structure 3258 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1060500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGACAATTCACCAAGTTATGATGAACACTACGATCTTGGTTACCAGCCTTCACCTTCTTCTGTGGATCAAACTGATCATTCGCTCGCGGAAACTTCAACAAGTGGCGATTCTTTCATGTACCGCCGGACAAATTCAGAGACCTCGGCTTACTCCGAGTCTGTGGATGACAGCAGTTACTCTGGTGAAGCTTCTCCTTGGTTCTACCCAAATGTAAAATCCAATCCGCTGTCTCTTACCAGACTTGGATTGAAGCAGTACAATAAGAATGCTTCCGATGATAAGCTTGAAGATCACAAAGCTGTTGATTCAGAGCTTGAGATGATGAAAGAAAGATTTGCAAAACTCTTGTTGGGTGAAGACATGTCAGGAAGTGGGAAAGGTGTATGCACCGCGGTCACGATCTCAAATTCGATAACCAACCTTTATGCCACGGTGTTTGGACAAAACTTGAGGCTAGAGCCATTGAATCCTGAGAAGAAAGCAATGTGGAAGAGGGAGATGAACTGTCTTTTATCAGTCTGTGATTACATGGTAGAACTCATCCCCATATCACAAAACTTACAAGAGGGGGCACAAGTGGAGTTGATGGAAAGCAGGCCAAGATCGGATCTTTATATCAATCTTCCAGCTTTGCAAAAGCTTGATGCAATGCTCATTGAAGTACTTGATAGTTTCCAGGATACAGAATTCTGGTATGCAGAAAAAGGGAGCATGTCTTCAAATTCAACGCGAACAGGATCTTTTCGTAGGGTGGTTGTTCAGCGGAATGAGGAGAAATGGTGGTTGCCTATTCCATGCGTTCCTCCGGGAGGCCTGTCTGAGAAGGCAAGGAAGCTTTTGAAACACAAACGCGACAGTTGTAATCAAATTCATAAAGCAGCCATGGCAATCAACAGCAGCATTCTTGCCGAGATGGAAATCCCAGACTCATACATGTCAACTCTTCCGAAGAGCGGAAAAGCTTGTCTGGGAGACTCCATATACCACTACATGTGCACGACGGACAAGTTCTCCCCAGACCAACTTCTGGACTGCCTCGACATAGCAACTGAACTGGAAGCACTTATTCTTGCAGACAGGGTCGAAGCCTCGATGTACACGTGGAGGCGTAAAGCGTGCATGAGCAACTCAAAATCTTCCTGGATCATGGTGAAAGATCTGATGTCAGAAACAGAATGGAATGACAAGAATTATGTTTTGGCAGAGAGAGCCGAGTGCTTGCTGTTCTGTTTGAAGCAGAGATATCCTGAGCTCTCTCAGACATCCTTGGACACATGCAAGATCCAATGCAATAGGGATGTGGGGCAGGCAGTGCTAGAGAGCTACTCAAGAGTGTTGGAGAGTTTGGCATTCAACATTGTTGCCTGGATTGAAGATGTAATTTGTGTTGACAGATCCGCGAGAAACCAAGACAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0009505 GO:0009531 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009664 GO:0009814 GO:0009817 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016043 GO:0016049 GO:0017016 GO:0017048 GO:0019899 GO:0021700 GO:0022622 GO:0030154 GO:0030312 GO:0031267 GO:0032501 GO:0032502 GO:0040007 GO:0043207 GO:0044424 GO:0044464 GO:0045087 GO:0045229 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051020 GO:0051704 GO:0051707 GO:0060560 GO:0065007 GO:0065009 GO:0071554 GO:0071555 GO:0071669 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0098542 GO:0098772 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PRONE

Protein Analysis

474

Amino Acids

53.6

Weight (kDa)

4.99

Isoelectric Point (pI)

61.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRONE	PF03759	104 - 469	2.6e-173	PRONE (Plant-specific Rop nucleotide exchanger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01700 AT1G01700 AT1G01700 AT1G01700 AT1G01700 AT4G00460 AT4G00460 AT4G00460 AT4G00460
fragaria_vesca	FvH4_7g14260 FvH4_7g14260 FvH4_7g14260 FvH4_7g14260
malus_domestica	MD01G1060500.v1.1 MD07G1137200.v1.1
prunus_persica	Prupe.2G170400_v2.0.a1
pyrus_communis	pycom07g13500
rosa_chinensis	RchiOBHm_Chr1g0355251
rosa_laevigata	RLG00000028167
rosa_multiflora	Rmu_sc0003115.1_g000043
rosa_roxburghii	Rroxscaffold_4G00300560
rosa_rugosa	Rorug01G0245800 Rorug01G0245800
rosa_samantha	Rh1AG258200 Rh1BG227800 Rh1CG241900 Rh1DG255600
rosa_wichuraiana	Rw1G022790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1030
AccBSI	CCGCTC	1 cut(s)	957
AccII	CGCG	5 cut(s)	89, 395, 733, 855, 1403
AciI	CCGC	8 cut(s)	89, 124, 227, 393, 395, 766, 957, 1401
AclWI	GGATC	6 cut(s)	75, 618, 748, 1154, 1279, 1392
AcsI	RAATTY	5 cut(s)	133, 409, 689, 724, 870
AcvI	CACGTG	1 cut(s)	1104
AfaI	GTAC	4 cut(s)	122, 260, 666, 1100
AfiI	CCNNNNNNNGG	3 cut(s)	812, 956, 1030
AflIII	ACRYGT	4 cut(s)	363, 936, 993, 1101
AjnI	CCWGG	3 cut(s)	678, 1142, 1367
AjuI	GAANNNNNNNTTGG	2 cut(s)	1184, 1216
Alw21I	GWGCWC	2 cut(s)	1001, 1257
Alw26I	GTCTC	3 cut(s)	134, 237, 969
Alw44I	GTGCAC	1 cut(s)	997
AlwI	GGATC	6 cut(s)	75, 618, 748, 1154, 1279, 1392
AoxI	GGCC	2 cut(s)	599, 814
ApaLI	GTGCAC	1 cut(s)	997
ApeKI	GCWGC	2 cut(s)	881, 900
ApoI	RAATTY	5 cut(s)	133, 409, 689, 724, 870
AsuC2I	CCSGG	1 cut(s)	810
AsuHPI	GGTGA	5 cut(s)	3, 45, 197, 368, 1165
BaeGI	GKGCMC	2 cut(s)	577, 1001
BanII	GRGCYC	1 cut(s)	1257
BbrPI	CACGTG	1 cut(s)	1104
BbsI	GAAGAC	2 cut(s)	366, 711
Bbv12I	GWGCWC	2 cut(s)	1001, 1257
BbvI	GCAGC	2 cut(s)	893, 912
BccI	CCATC	2 cut(s)	583, 910
BciT130I	CCWGG	3 cut(s)	680, 1144, 1369
BciVI	GTATCC	1 cut(s)	676
BclI	TGATCA	1 cut(s)	76
BcnI	CCSGG	1 cut(s)	810
BcoDI	GTCTC	3 cut(s)	134, 237, 969
BfaI	CTAG	2 cut(s)	458, 1319
BfuI	GTATCC	1 cut(s)	676
BglII	AGATCT	1 cut(s)	1159
BisI	GCNGC	2 cut(s)	882, 901
BlsI	GCNGC	2 cut(s)	883, 902
BmcAI	AGTACT	1 cut(s)	666
Bme1390I	CCNGG	4 cut(s)	680, 810, 1144, 1369
BmrFI	CCNGG	4 cut(s)	680, 810, 1144, 1369
BmsI	GCATC	1 cut(s)	637
BpiI	GAAGAC	2 cut(s)	366, 711
BplI	GAGNNNNNCTC	2 cut(s)	1313, 1345
Bpu10I	CCTNAGC	1 cut(s)	1251
BpuEI	CTTGAG	3 cut(s)	338, 472, 1315
BpuMI	CCSGG	1 cut(s)	810
BsaAI	YACGTR	1 cut(s)	1104
BsaBI	GATNNNNATC	1 cut(s)	920
BsaI	GGTCTC	1 cut(s)	134
BsaJI	CCNNGG	6 cut(s)	144, 197, 393, 432, 885, 1269
BsaXI	ACNNNNNCTCC	4 cut(s)	767, 797, 998, 1028
Bsc4I	CCNNNNNNNGG	3 cut(s)	812, 956, 1030
Bse1I	ACTGG	1 cut(s)	1062
Bse3DI	GCAATG	2 cut(s)	492, 657
Bse8I	GATNNNNATC	1 cut(s)	920
BseBI	CCWGG	3 cut(s)	680, 1144, 1369
BseDI	CCNNGG	6 cut(s)	144, 197, 393, 432, 885, 1269
BseGI	GGATG	4 cut(s)	172, 543, 1265, 1306
BseJI	GATNNNNATC	1 cut(s)	920
BseLI	CCNNNNNNNGG	3 cut(s)	812, 956, 1030
BseMI	GCAATG	2 cut(s)	492, 657
BseMII	CTCAG	4 cut(s)	465, 813, 1242, 1274
BseNI	ACTGG	1 cut(s)	1062
BseRI	GAGGAG	1 cut(s)	788
BseSI	GKGCMC	2 cut(s)	577, 1001
BseXI	GCAGC	2 cut(s)	893, 912
Bsh1236I	CGCG	5 cut(s)	89, 395, 733, 855, 1403
BshFI	GGCC	2 cut(s)	601, 816
BsiHKAI	GWGCWC	2 cut(s)	1001, 1257
BsiSI	CCGG	2 cut(s)	127, 809
BslI	CCNNNNNNNGG	3 cut(s)	812, 956, 1030
BsmAI	GTCTC	3 cut(s)	134, 237, 969
BsmI	GAATGC	3 cut(s)	274, 903, 1352
BsnI	GGCC	2 cut(s)	601, 816
Bso31I	GGTCTC	1 cut(s)	134
Bsp1286I	GDGCHC	3 cut(s)	577, 1001, 1257
Bsp1407I	TGTACA	1 cut(s)	1098
Bsp19I	CCATGG	1 cut(s)	885
BspACI	CCGC	8 cut(s)	89, 124, 227, 393, 395, 766, 957, 1401
BspANI	GGCC	2 cut(s)	601, 816
BspCNI	CTCAG	4 cut(s)	466, 814, 1243, 1273
BspFNI	CGCG	5 cut(s)	89, 395, 733, 855, 1403
BspPI	GGATC	6 cut(s)	75, 618, 748, 1154, 1279, 1392
BspQI	GCTCTTC	1 cut(s)	947
BspTNI	GGTCTC	1 cut(s)	134
BsrBI	CCGCTC	1 cut(s)	957
BsrDI	GCAATG	2 cut(s)	492, 657
BsrGI	TGTACA	1 cut(s)	1098
BsrI	ACTGG	1 cut(s)	1062
BssECI	CCNNGG	6 cut(s)	144, 197, 393, 432, 885, 1269
BssT1I	CCWWGG	3 cut(s)	197, 885, 1269
Bst2UI	CCWGG	3 cut(s)	680, 1144, 1369
Bst4CI	ACNGT	3 cut(s)	436, 509, 860
Bst6I	CTCTTC	3 cut(s)	488, 947, 951
BstAPI	GCANNNNNTGC	1 cut(s)	1315
BstAUI	TGTACA	1 cut(s)	1098
BstBAI	YACGTR	1 cut(s)	1104
BstC8I	GCNNGC	5 cut(s)	87, 599, 1120, 1223, 1311
BstDEI	CTNAG	4 cut(s)	474, 822, 1251, 1260
BstDSI	CCRYGG	3 cut(s)	393, 432, 885
BstEII	GGTNACC	1 cut(s)	41
BstENI	CCTNNNNNAGG	1 cut(s)	810
BstF5I	GGATG	4 cut(s)	172, 543, 1265, 1306
BstFNI	CGCG	5 cut(s)	89, 395, 733, 855, 1403
BstMAI	GTCTC	3 cut(s)	134, 237, 969
BstMWI	GCNNNNNNNGC	3 cut(s)	887, 1211, 1315
BstNI	CCWGG	3 cut(s)	680, 1144, 1369
BstNSI	RCATGY	5 cut(s)	367, 718, 940, 997, 1281
BstPI	GGTNACC	1 cut(s)	41
BstSCI	CCNGG	4 cut(s)	678, 808, 1142, 1367
BstSLI	GKGCMC	2 cut(s)	577, 1001
BstUI	CGCG	5 cut(s)	89, 395, 733, 855, 1403
BstV1I	GCAGC	2 cut(s)	893, 912
BstV2I	GAAGAC	2 cut(s)	366, 711
BstX2I	RGATCY	5 cut(s)	610, 740, 1159, 1284, 1397
BstYI	RGATCY	5 cut(s)	610, 740, 1159, 1284, 1397
BsuI	GTATCC	1 cut(s)	676
BsuRI	GGCC	2 cut(s)	601, 816
BtgI	CCRYGG	3 cut(s)	393, 432, 885
BtsCI	GGATG	4 cut(s)	172, 543, 1265, 1306
BtsI	GCAGTG	1 cut(s)	1320
BtsIMutI	CAGTG	1 cut(s)	1320
Cac8I	GCNNGC	5 cut(s)	87, 599, 1120, 1223, 1311
Cfr42I	CCGCGG	1 cut(s)	396
Csp6I	GTAC	4 cut(s)	121, 259, 665, 1099
CspCI	CAANNNNNGTGG	2 cut(s)	421, 456
CviQI	GTAC	4 cut(s)	121, 259, 665, 1099
DdeI	CTNAG	4 cut(s)	474, 822, 1251, 1260
Eam1104I	CTCTTC	3 cut(s)	488, 947, 951
EarI	CTCTTC	3 cut(s)	488, 947, 951
Ecl136II	GAGCTC	1 cut(s)	1255
Eco130I	CCWWGG	3 cut(s)	197, 885, 1269
Eco147I	AGGCCT	1 cut(s)	816
Eco24I	GRGCYC	1 cut(s)	1257
Eco31I	GGTCTC	1 cut(s)	134
Eco32I	GATATC	1 cut(s)	1247
Eco53kI	GAGCTC	1 cut(s)	1255
Eco72I	CACGTG	1 cut(s)	1104
Eco91I	GGTNACC	1 cut(s)	41
EcoICRI	GAGCTC	1 cut(s)	1255
EcoNI	CCTNNNNNAGG	1 cut(s)	810
EcoO65I	GGTNACC	1 cut(s)	41
EcoRI	GAATTC	1 cut(s)	689
EcoRII	CCWGG	3 cut(s)	678, 1142, 1367
EcoRV	GATATC	1 cut(s)	1247
EcoT14I	CCWWGG	3 cut(s)	197, 885, 1269
EcoT38I	GRGCYC	1 cut(s)	1257
ErhI	CCWWGG	3 cut(s)	197, 885, 1269
FbaI	TGATCA	1 cut(s)	76
Fnu4HI	GCNGC	2 cut(s)	882, 901
FokI	GGATG	4 cut(s)	179, 530, 1252, 1313
FriOI	GRGCYC	1 cut(s)	1257
Fsp4HI	GCNGC	2 cut(s)	882, 901
FspBI	CTAG	2 cut(s)	458, 1319
GluI	GCNGC	2 cut(s)	882, 901
HaeIII	GGCC	2 cut(s)	601, 816
HapII	CCGG	2 cut(s)	127, 809
HincII	GTYRAC	2 cut(s)	942, 1393
HindII	GTYRAC	2 cut(s)	942, 1393
HindIII	AAGCTT	5 cut(s)	189, 284, 641, 836, 963
HinfI	GANTC	6 cut(s)	110, 158, 308, 469, 929, 977
HpaII	CCGG	2 cut(s)	127, 809
HphI	GGTGA	5 cut(s)	3, 45, 197, 368, 1165
Hpy166II	GTNNAC	4 cut(s)	942, 999, 1101, 1393
Hpy188III	TCNNGA	6 cut(s)	369, 400, 473, 1031, 1250, 1332
Hpy8I	GTNNAC	4 cut(s)	942, 999, 1101, 1393
Hpy99I	CGWCG	1 cut(s)	1006
HpyAV	CCTTC	3 cut(s)	60, 66, 820
HpyCH4III	ACNGT	3 cut(s)	436, 509, 860
HpyCH4IV	ACGT	1 cut(s)	1103
HpyF10VI	GCNNNNNNNGC	3 cut(s)	887, 1211, 1315
HpyF3I	CTNAG	4 cut(s)	474, 822, 1251, 1260
HpySE526I	ACGT	1 cut(s)	1103
Ksp22I	TGATCA	1 cut(s)	76
KspI	CCGCGG	1 cut(s)	396
LguI	GCTCTTC	1 cut(s)	947
LmnI	GCTCC	1 cut(s)	711
Lsp1109I	GCAGC	2 cut(s)	893, 912
LweI	GCATC	1 cut(s)	637
MaeI	CTAG	2 cut(s)	458, 1319
MaeII	ACGT	1 cut(s)	1103
MaeIII	GTNAC	3 cut(s)	41, 176, 397
MbiI	CCGCTC	1 cut(s)	957
MflI	RGATCY	5 cut(s)	610, 740, 1159, 1284, 1397
MhlI	GDGCHC	3 cut(s)	577, 1001, 1257
MluCI	AATT	8 cut(s)	7, 133, 409, 689, 724, 870, 1192, 1383
MlyI	GAGTC	3 cut(s)	167, 923, 971
MmeI	TCCRAC	1 cut(s)	1320
MspA1I	CMGCKG	3 cut(s)	229, 395, 766
MspI	CCGG	2 cut(s)	127, 809
MspR9I	CCNGG	4 cut(s)	680, 810, 1144, 1369
Mva1269I	GAATGC	3 cut(s)	274, 903, 1352
MvaI	CCWGG	3 cut(s)	680, 1144, 1369
MvnI	CGCG	5 cut(s)	89, 395, 733, 855, 1403
MwoI	GCNNNNNNNGC	3 cut(s)	887, 1211, 1315
NciI	CCSGG	1 cut(s)	810
NcoI	CCATGG	1 cut(s)	885
NmeAIII	GCCGAG	3 cut(s)	125, 937, 1240
NmuCI	GTSAC	1 cut(s)	397
NspI	RCATGY	5 cut(s)	367, 718, 940, 997, 1281
PceI	AGGCCT	1 cut(s)	816
PciI	ACATGT	3 cut(s)	363, 936, 993
PciSI	GCTCTTC	1 cut(s)	947
PcsI	WCGNNNNNNNCGW	1 cut(s)	1100
PctI	GAATGC	3 cut(s)	274, 903, 1352
PfeI	GAWTC	3 cut(s)	110, 308, 469
PflFI	GACNNNGTC	1 cut(s)	1082
PflMI	CCANNNNNTGG	1 cut(s)	1030
PfoI	TCCNGGA	3 cut(s)	678, 808, 1142
PkrI	GCNGC	2 cut(s)	883, 902
PleI	GAGTC	3 cut(s)	166, 923, 971
PmaCI	CACGTG	1 cut(s)	1104
PmlI	CACGTG	1 cut(s)	1104
PpsI	GAGTC	3 cut(s)	166, 923, 971
Ppu21I	YACGTR	1 cut(s)	1104
PscI	ACATGT	3 cut(s)	363, 936, 993
Psp124BI	GAGCTC	1 cut(s)	1257
Psp6I	CCWGG	3 cut(s)	678, 1142, 1367
PspCI	CACGTG	1 cut(s)	1104
PspEI	GGTNACC	1 cut(s)	41
PspGI	CCWGG	3 cut(s)	678, 1142, 1367
PsuI	RGATCY	5 cut(s)	610, 740, 1159, 1284, 1397
PsyI	GACNNNGTC	1 cut(s)	1082
RsaI	GTAC	4 cut(s)	122, 260, 666, 1100
RsaNI	GTAC	4 cut(s)	121, 259, 665, 1099
SacI	GAGCTC	1 cut(s)	1257
SacII	CCGCGG	1 cut(s)	396
SapI	GCTCTTC	1 cut(s)	947
SatI	GCNGC	2 cut(s)	882, 901
ScaI	AGTACT	1 cut(s)	666
SchI	GAGTC	3 cut(s)	167, 923, 971
ScrFI	CCNGG	4 cut(s)	680, 810, 1144, 1369
SduI	GDGCHC	3 cut(s)	577, 1001, 1257
SfaNI	GCATC	1 cut(s)	637
Sfr303I	CCGCGG	1 cut(s)	396
SgrBI	CCGCGG	1 cut(s)	396
SmlI	CTYRAG	3 cut(s)	317, 451, 1330
SmoI	CTYRAG	3 cut(s)	317, 451, 1330
Sse9I	AATT	8 cut(s)	7, 133, 409, 689, 724, 870, 1192, 1383
SseBI	AGGCCT	1 cut(s)	816
SsiI	CCGC	8 cut(s)	89, 124, 227, 393, 395, 766, 957, 1401
SspMI	CTAG	2 cut(s)	458, 1319
SstI	GAGCTC	1 cut(s)	1257
StuI	AGGCCT	1 cut(s)	816
StyD4I	CCNGG	4 cut(s)	678, 808, 1142, 1367
StyI	CCWWGG	3 cut(s)	197, 885, 1269
TaaI	ACNGT	3 cut(s)	436, 509, 860
TaiI	ACGT	1 cut(s)	1106
TaqI	TCGA	4 cut(s)	413, 1041, 1086, 1094
TasI	AATT	8 cut(s)	7, 133, 409, 689, 724, 870, 1192, 1383
TatI	WGTACW	3 cut(s)	258, 664, 1098
TfiI	GAWTC	3 cut(s)	110, 308, 469
TscAI	CASTG	1 cut(s)	1320
TseFI	GTSAC	1 cut(s)	397
TseI	GCWGC	2 cut(s)	881, 900
Tsp45I	GTSAC	1 cut(s)	397
TspDTI	ATGAA	5 cut(s)	39, 106, 341, 518, 863
TspGWI	ACGGA	1 cut(s)	1019
TspRI	CASTG	1 cut(s)	1320
Tth111I	GACNNNGTC	1 cut(s)	1082
Van91I	CCANNNNNTGG	1 cut(s)	1030
VneI	GTGCAC	1 cut(s)	997
XagI	CCTNNNNNAGG	1 cut(s)	810
XapI	RAATTY	5 cut(s)	133, 409, 689, 724, 870
XceI	RCATGY	5 cut(s)	367, 718, 940, 997, 1281
XspI	CTAG	2 cut(s)	458, 1319
ZrmI	AGTACT	1 cut(s)	666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.