AT4G08700

Member of a family of proteins related to PUP1, a purine transporter. May be involved in the transport of purine and purine derivatives such as cytokinins, across the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010694
Physical Location & Seq
Reverse (-)
5565773 .. 5567458
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08700.1

Sequence Viewer

Length: 1086 bp
ATGGATGAAGAAGAAGCAATGTTACTGCTAAAGGAAGAAGATGAAGGAACAAGAAGAACTAGTGTTCCTACACAACTCATGAAACTAAAACGAACCCATTGGTGGATTCTTGTCTTTATAAGTATCTTCTTCCTCATCTCTGCACAAGCCATTGCTGTTCTTCTTGGTCGGTTTTATTACAACGAAGGTGGAAACAGTAAATGGATCTCTACTCTTGTCCAAACTTGTGGTTTTCCGATTCTCTATCTCCCTCTTTGTTTCCTTCCTGCTTCACACTCTTCTTCATCTTCTTGTTCTTTCAAGACTCTGGTTTGGATTTATCTTTCTCTTGGTTTTGCTATTGGTTTAGACAATCTTTTATACTCTTTTGGTCTTTTGTATCTCTCTGCTTCAACTTATTCGATTCTTTGTTCTTCACAGTTAGCTTTCAATGGTGTCTTCTCTTATTACATCAATTCTCAGAAAATCACTTGTTTGATTCTCTTCTCTGTGTTGTTTCTCTCTGTCTCTGCTGTGTTGGTTTCTCTTGACGATGATTCCAATAGCCCATCAGGAGATTCTAAGTGGAGTTACTTGATTGGTTGTTTATGTACTGTTTTTGCTTCTCTTATCTATTCTCTTCAGCTCTCTCTTATGCAGTTTTCTTTCGAGAATGTTCTCAAGAGTGAGACGTTCTCTATGGTTCTCGAGATGCAAATCTATACGTCGCTTGTGGCTTCTTGTGTTGCGGTTATCGGGTTGTTTGCGAGCGGGGAATGGATGTTGTTGAGTGTGGAGATGGAAGAGTTTCACGAGGGTCAAGTCATTTATGTTTTGACTTTGGTTGGGACCGCGGTTTCGTGGCAATTGGGTTCTGTAGGAGCCGTGGCGCTTATATTTCTGGTTTCTTCGCTGTTTTCGAACCTTATTGGTACACTCTCACTCATTGTTACGCCTCTTGCAGCCATTGCGGTGTTCCACGACAAGCTGACTGAGGTTAAGATGGTCGCGATGCTCATTGCCTTCATGGGGTTCGGGTTTTATATCTACCAGAATTATCTTGATGACCTGAAAGTACAAAGAGCACGAGAAGCTCAGGCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

361

Amino Acids

40.14

Weight (kDa)

4.82

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 36 - 350 1.8e-123 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 750
AccII CGCG 2 cut(s) 833, 989
AciI CCGC 5 cut(s) 728, 750, 831, 833, 950
AclWI GGATC 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 3 cut(s) 592, 913, 1056
AfiI CCNNNNNNNGG 2 cut(s) 102, 1008
AgsI TTSAA 3 cut(s) 301, 393, 430
AhlI ACTAGT 1 cut(s) 59
AloI GAACNNNNNNTCC 2 cut(s) 49, 81
AluBI AGCT 4 cut(s) 425, 625, 967, 1073
AluI AGCT 4 cut(s) 425, 625, 967, 1073
Alw21I GWGCWC 1 cut(s) 1066
Alw26I GTCTC 2 cut(s) 511, 662
AlwI GGATC 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 686
ApeKI GCWGC 1 cut(s) 941
ArsI GACNNNNNNTTYG 2 cut(s) 820, 852
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 1 cut(s) 871
AspS9I GGNCC 1 cut(s) 828
AsuII TTCGAA 1 cut(s) 899
AvaI CYCGRG 1 cut(s) 686
AvaII GGWCC 1 cut(s) 828
BarI GAAGNNNNNNTAC 1 cut(s) 38
BauI CACGAG 2 cut(s) 791, 1065
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 1 cut(s) 1066
BbvI GCAGC 1 cut(s) 953
BccI CCATC 3 cut(s) 556, 772, 976
BceAI ACGGC 1 cut(s) 848
BcoDI GTCTC 2 cut(s) 511, 662
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 855
BfoI RGCGCY 1 cut(s) 872
BisI GCNGC 1 cut(s) 942
BlsI GCNGC 1 cut(s) 943
Bme18I GGWCC 1 cut(s) 828
BmeT110I CYCGRG 1 cut(s) 686
BmgT120I GGNCC 1 cut(s) 828
BmiI GGNNCC 2 cut(s) 829, 862
BmsI GCATC 2 cut(s) 681, 981
BpiI GAAGAC 1 cut(s) 430
BplI GAGNNNNNCTC 2 cut(s) 659, 691
Bpu10I CCTNAGC 1 cut(s) 1074
Bpu14I TTCGAA 1 cut(s) 899
BpuEI CTTGAG 1 cut(s) 644
BsaBI GATNNNNATC 1 cut(s) 695
BsaJI CCNNGG 2 cut(s) 831, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 1008
Bse3DI GCAATG 4 cut(s) 24, 150, 945, 996
Bse8I GATNNNNATC 1 cut(s) 695
BseDI CCNNGG 2 cut(s) 831, 864
BseGI GGATG 2 cut(s) 10, 765
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 102, 1008
BseMI GCAATG 4 cut(s) 24, 150, 945, 996
BseMII CTCAG 2 cut(s) 473, 963
BseXI GCAGC 1 cut(s) 953
BsgI GTGCAG 1 cut(s) 126
Bsh1236I CGCG 2 cut(s) 833, 989
BsiHKAI GWGCWC 1 cut(s) 1066
BsiHKCI CYCGRG 1 cut(s) 686
BslFI GGGAC 1 cut(s) 841
BslI CCNNNNNNNGG 2 cut(s) 102, 1008
BsmAI GTCTC 2 cut(s) 511, 662
BsmBI CGTCTC 1 cut(s) 662
BsmFI GGGAC 1 cut(s) 841
BsoBI CYCGRG 1 cut(s) 686
Bsp119I TTCGAA 1 cut(s) 899
Bsp1286I GDGCHC 1 cut(s) 1066
Bsp143I GATC 1 cut(s) 204
Bsp68I TCGCGA 1 cut(s) 989
BspACI CCGC 5 cut(s) 728, 750, 831, 833, 950
BspCNI CTCAG 2 cut(s) 472, 964
BspFNI CGCG 2 cut(s) 833, 989
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 829, 862
BspPI GGATC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 899
BsrBI CCGCTC 1 cut(s) 750
BsrDI GCAATG 4 cut(s) 24, 150, 945, 996
BssECI CCNNGG 2 cut(s) 831, 864
BssMI GATC 1 cut(s) 204
BssSI CACGAG 2 cut(s) 791, 1065
Bst2BI CACGAG 2 cut(s) 791, 1065
Bst4CI ACNGT 3 cut(s) 197, 420, 595
Bst6I CTCTTC 4 cut(s) 283, 488, 624, 777
BstAPI GCANNNNNTGC 1 cut(s) 947
BstBI TTCGAA 1 cut(s) 899
BstC8I GCNNGC 1 cut(s) 748
BstDEI CTNAG 4 cut(s) 459, 561, 972, 1074
BstDSI CCRYGG 2 cut(s) 831, 864
BstF5I GGATG 2 cut(s) 10, 765
BstFNI CGCG 2 cut(s) 833, 989
BstH2I RGCGCY 1 cut(s) 872
BstHHI GCGC 1 cut(s) 871
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 2 cut(s) 511, 662
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 947, 1070
BstSFI CTRYAG 1 cut(s) 855
BstUI CGCG 2 cut(s) 833, 989
BstV1I GCAGC 1 cut(s) 953
BstV2I GAAGAC 1 cut(s) 430
BstX2I RGATCY 1 cut(s) 204
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 1 cut(s) 204
BtgI CCRYGG 2 cut(s) 831, 864
BtgZI GCGATG 1 cut(s) 1004
BtsCI GGATG 2 cut(s) 10, 765
BtuMI TCGCGA 1 cut(s) 989
Cac8I GCNNGC 1 cut(s) 748
CciI TCATGA 1 cut(s) 78
CfoI GCGC 1 cut(s) 871
Cfr13I GGNCC 1 cut(s) 828
Cfr42I CCGCGG 1 cut(s) 834
Csp6I GTAC 3 cut(s) 591, 912, 1055
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 2 cut(s) 79, 1006
CviQI GTAC 3 cut(s) 591, 912, 1055
DdeI CTNAG 4 cut(s) 459, 561, 972, 1074
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Eam1104I CTCTTC 4 cut(s) 283, 488, 624, 777
EarI CTCTTC 4 cut(s) 283, 488, 624, 777
Eco47I GGWCC 1 cut(s) 828
Eco57I CTGAAG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 686
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 2 cut(s) 82, 1009
FaqI GGGAC 1 cut(s) 841
FatI CATG 2 cut(s) 78, 1005
FauI CCCGC 1 cut(s) 743
Fnu4HI GCNGC 1 cut(s) 942
FokI GGATG 2 cut(s) 17, 772
Fsp4HI GCNGC 1 cut(s) 942
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 870
GluI GCNGC 1 cut(s) 942
HaeII RGCGCY 1 cut(s) 872
HhaI GCGC 1 cut(s) 871
Hin1II CATG 2 cut(s) 82, 1009
Hin6I GCGC 1 cut(s) 869
HinP1I GCGC 1 cut(s) 869
HinfI GANTC 7 cut(s) 106, 238, 304, 403, 478, 536, 557
Hpy166II GTNNAC 1 cut(s) 914
Hpy188I TCNGA 2 cut(s) 237, 462
Hpy8I GTNNAC 1 cut(s) 914
Hpy99I CGWCG 1 cut(s) 709
HpyAV CCTTC 4 cut(s) 38, 179, 272, 1012
HpyCH4III ACNGT 3 cut(s) 197, 420, 595
HpyCH4IV ACGT 2 cut(s) 671, 704
HpyCH4V TGCA 4 cut(s) 143, 637, 694, 941
HpyF10VI GCNNNNNNNGC 2 cut(s) 947, 1070
HpyF3I CTNAG 4 cut(s) 459, 561, 972, 1074
HpySE526I ACGT 2 cut(s) 671, 704
Hsp92II CATG 2 cut(s) 82, 1009
HspAI GCGC 1 cut(s) 869
KspI CCGCGG 1 cut(s) 834
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 860
LpnPI CCDG 7 cut(s) 279, 293, 537, 866, 1043, 1061, 1061
Lsp1109I GCAGC 1 cut(s) 953
LweI GCATC 2 cut(s) 681, 981
MaeI CTAG 1 cut(s) 60
MaeII ACGT 2 cut(s) 671, 704
MaeIII GTNAC 3 cut(s) 21, 569, 928
MalI GATC 1 cut(s) 206
MbiI CCGCTC 1 cut(s) 750
MboI GATC 1 cut(s) 204
MfeI CAATTG 1 cut(s) 845
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 1066
MluCI AATT 3 cut(s) 454, 845, 1033
MlyI GAGTC 1 cut(s) 298
MnlI CCTC 5 cut(s) 143, 261, 787, 945, 967
MroXI GAANNNNTTC 1 cut(s) 786
MseI TTAA 1 cut(s) 978
MslI CAYNNNNRTG 1 cut(s) 950
MspA1I CMGCKG 1 cut(s) 833
MunI CAATTG 1 cut(s) 845
MvnI CGCG 2 cut(s) 833, 989
MwoI GCNNNNNNNGC 2 cut(s) 947, 1070
NdeII GATC 1 cut(s) 204
NlaIII CATG 2 cut(s) 82, 1009
NlaIV GGNNCC 2 cut(s) 829, 862
NruI TCGCGA 1 cut(s) 989
NspV TTCGAA 1 cut(s) 899
PaeR7I CTCGAG 1 cut(s) 686
PagI TCATGA 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 896
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 6 cut(s) 106, 238, 403, 478, 536, 557
PkrI GCNGC 1 cut(s) 943
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
PsiI TTATAA 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 829, 862
PspPI GGNCC 1 cut(s) 828
PsuI RGATCY 1 cut(s) 204
RruI TCGCGA 1 cut(s) 989
RsaI GTAC 3 cut(s) 592, 913, 1056
RsaNI GTAC 3 cut(s) 591, 912, 1055
RseI CAYNNNNRTG 1 cut(s) 950
SacII CCGCGG 1 cut(s) 834
SaqAI TTAA 1 cut(s) 978
SatI GCNGC 1 cut(s) 942
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 1 cut(s) 828
SchI GAGTC 1 cut(s) 298
SduI GDGCHC 1 cut(s) 1066
SfaNI GCATC 2 cut(s) 681, 981
SfcI CTRYAG 1 cut(s) 855
Sfr274I CTCGAG 1 cut(s) 686
Sfr303I CCGCGG 1 cut(s) 834
SfuI TTCGAA 1 cut(s) 899
SgrBI CCGCGG 1 cut(s) 834
SinI GGWCC 1 cut(s) 828
SlaI CTCGAG 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 2 cut(s) 659, 686
SmoI CTYRAG 2 cut(s) 659, 686
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 3 cut(s) 454, 845, 1033
SsiI CCGC 5 cut(s) 728, 750, 831, 833, 950
SspMI CTAG 1 cut(s) 60
TaaI ACNGT 3 cut(s) 197, 420, 595
TaiI ACGT 2 cut(s) 674, 707
TaqI TCGA 4 cut(s) 401, 648, 687, 899
TasI AATT 3 cut(s) 454, 845, 1033
TatI WGTACW 2 cut(s) 590, 1054
TfiI GAWTC 6 cut(s) 106, 238, 403, 478, 536, 557
Tru1I TTAA 1 cut(s) 978
Tru9I TTAA 1 cut(s) 978
TseI GCWGC 1 cut(s) 941
TspDTI ATGAA 5 cut(s) 21, 57, 95, 273, 994
VpaK11BI GGWCC 1 cut(s) 828
XhoI CTCGAG 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.