RLG00000003256

Purine nucleobase transmembrane transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010694
Physical Location & Seq
Reverse (-)
46201941 .. 46204000
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003256

Sequence Viewer

Length: 1287 bp
ATGGAAGAAGAAGAAGTAACTTACACGGGTCTCACACCAGTTGGACTTGACGTCTACATTGGAGACTGGAGACCAGATTCACCAACAAACACCTGCCTAAACTCTTCTGGTAACCGATTGGAATCAACTAATTACAAGTGGTGGCTTCGTGTGACCATCTATACCGTCTTTCTTCTCGCCGGCCAATCTACAGCGACACTCTTGGGAAGATTGTACTATGATAAAGGTGGCAATAGCAAATGGATGGCAACTTTTGTTCAGTCCGCCGGCTTCCCAGTTCTTCTTCCTCTCCTCTGTTTCTTTTCATCAACAACCACATTGACTACAAACTCTGTTGGTAGTACTACTTCATCATCTGCTTCCAAACTACCAAAACTCACAACAATGGTATTCCTCTACGTTGCCCTTGGCTTGCTTTTGACGGCAGACAACATGATGTACTCTTACGGGCTTCTCTTTCTCCCCGTCTCTACTTACTCTTTACTATGTGCAACCCAATTGGCCTTCAACGCGTTGTTCTCTTTCTTTCTGAACTCTCAAAAATTTACTCCTTTTATACTCAACTCTCTTGTCCTTGTCTCTATGTCTGCCTCCGTTCTCGCCATCAATTCTGACTCCGAAAACACTACCGATCACATCTCTAGAGGTAAGTATGTGATCGGTTTCTTGTGTACCCTCGGCGCCTCCGCGACTTACTCATTGTACCTTTCCCTTGTGCAACTTTCATTCCAGAAGGTGATAAAAAGAGAGACATTTTCTACTGTCTTGGATATGCAATTATACCCATCGTTTGTTGCGACATGTGGTTGTGTGGTAGGGCTTTTTGCAAGTGGGGAATGGAAAGGTTTGGAAAGTGAGATGAAGGAATACAAAGAAGGAAGAGTCTCTTATATAATGACCCTGCTCTGGACTGCTGTGTCATGGCAAATTTGTTCAGTAGGTTTGTTGGGGTTGATTTTTGAAGTGTCTTCTCTCTTCTCCAATGTCATCAGTACCTTGGGTTTGCCACTAGTTCCCATTCTTGCTGTTATCACTTTCCATGATAAAATGGATGCAATGAAGCATACCAAGTCGTTTTATGGCTTTAAAGACTTACGGGTTCTGGTTCTACGAAGAGATACATTTTATAATTGTTCTGTCACTGATTTCGTCTTTGCTGGTGGACAGAGGGTGACTACTCATCCTTTTGTTGACGACGGCGGGTGTCCAGGAGGGTCTGGCAGGGGTCGTGGTCAGAGGGTGGCTGCTGGGTCTCCGCTTGCTTTGTTTTGGGCCCAGTCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

429

Amino Acids

46.99

Weight (kDa)

7.99

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 50 - 355 1.1e-116 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1128
AarI CACCTGC 1 cut(s) 101
AasI GACNNNNNNGTC 1 cut(s) 916
AatII GACGTC 1 cut(s) 54
Acc36I ACCTGC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 54
AccII CGCG 2 cut(s) 512, 689
AciI CCGC 4 cut(s) 264, 687, 1200, 1256
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 2 cut(s) 542, 927
AcyI GRCGYC 2 cut(s) 51, 681
AfaI GTAC 6 cut(s) 215, 343, 440, 673, 704, 994
AfiI CCNNNNNNNGG 1 cut(s) 906
AflIII ACRYGT 2 cut(s) 510, 800
AgsI TTSAA 2 cut(s) 508, 962
AhdI GACNNNNNGTC 1 cut(s) 50
AhlI ACTAGT 1 cut(s) 1009
AjnI CCWGG 1 cut(s) 1207
Alw26I GTCTC 8 cut(s) 35, 57, 64, 472, 583, 743, 889, 1257
AoxI GGCC 3 cut(s) 181, 501, 1272
ApaI GGGCCC 1 cut(s) 1276
ApeKI GCWGC 1 cut(s) 1244
ApoI RAATTY 2 cut(s) 542, 927
AspLEI GCGC 1 cut(s) 683
AspS9I GGNCC 2 cut(s) 1272, 1273
AsuHPI GGTGA 3 cut(s) 72, 748, 1183
BaeGI GKGCMC 1 cut(s) 1276
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 1276
BarI GAAGNNNNNNTAC 2 cut(s) 331, 363
BbsI GAAGAC 1 cut(s) 960
BbvI GCAGC 1 cut(s) 1231
BccI CCATC 4 cut(s) 164, 238, 611, 793
BceAI ACGGC 2 cut(s) 438, 1213
BciT130I CCWGG 1 cut(s) 1209
BcoDI GTCTC 8 cut(s) 35, 57, 64, 472, 583, 743, 889, 1257
BcuI ACTAGT 1 cut(s) 1009
BfaI CTAG 2 cut(s) 642, 1010
BfmI CTRYAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 684
BfuAI ACCTGC 1 cut(s) 101
BisI GCNGC 1 cut(s) 1245
BlsI GCNGC 1 cut(s) 1246
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 1209
BmeRI GACNNNNNGTC 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 1272, 1273
BmiI GGNNCC 2 cut(s) 682, 1274
BmrFI CCNGG 1 cut(s) 1209
BmrI ACTGGG 2 cut(s) 269, 1270
BmsI GCATC 1 cut(s) 1042
BmuI ACTGGG 2 cut(s) 269, 1270
BpiI GAAGAC 1 cut(s) 960
BpmI CTGGAG 1 cut(s) 88
BsaBI GATNNNNATC 1 cut(s) 121
BsaHI GRCGYC 2 cut(s) 51, 681
BsaI GGTCTC 3 cut(s) 35, 64, 1257
BsaJI CCNNGG 3 cut(s) 406, 676, 996
Bsc4I CCNNNNNNNGG 1 cut(s) 906
Bse118I RCCGGY 2 cut(s) 179, 266
Bse1I ACTGG 4 cut(s) 38, 71, 275, 1276
Bse3DI GCAATG 1 cut(s) 1062
Bse8I GATNNNNATC 1 cut(s) 121
BseBI CCWGG 1 cut(s) 1209
BseDI CCNNGG 3 cut(s) 406, 676, 996
BseGI GGATG 3 cut(s) 249, 1057, 1180
BseJI GATNNNNATC 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 906
BseMI GCAATG 1 cut(s) 1062
BseNI ACTGG 4 cut(s) 38, 71, 275, 1276
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 1276
BseXI GCAGC 1 cut(s) 1231
BseYI CCCAGC 1 cut(s) 1247
Bsh1236I CGCG 2 cut(s) 512, 689
BshFI GGCC 3 cut(s) 183, 503, 1274
BshNI GGYRCC 1 cut(s) 680
BsiSI CCGG 2 cut(s) 180, 267
BslI CCNNNNNNNGG 1 cut(s) 906
BsmAI GTCTC 8 cut(s) 35, 57, 64, 472, 583, 743, 889, 1257
BsmBI CGTCTC 1 cut(s) 472
BsnI GGCC 3 cut(s) 183, 503, 1274
Bso31I GGTCTC 3 cut(s) 35, 64, 1257
Bsp120I GGGCCC 1 cut(s) 1272
Bsp1286I GDGCHC 1 cut(s) 1276
Bsp143I GATC 2 cut(s) 631, 657
BspACI CCGC 4 cut(s) 264, 687, 1200, 1256
BspANI GGCC 3 cut(s) 183, 503, 1274
BspFNI CGCG 2 cut(s) 512, 689
BspLI GGNNCC 2 cut(s) 682, 1274
BspMI ACCTGC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 680
BspTNI GGTCTC 3 cut(s) 35, 64, 1257
BsrDI GCAATG 1 cut(s) 1062
BsrFI RCCGGY 2 cut(s) 179, 266
BsrI ACTGG 4 cut(s) 38, 71, 275, 1276
BssAI RCCGGY 2 cut(s) 179, 266
BssECI CCNNGG 3 cut(s) 406, 676, 996
BssMI GATC 2 cut(s) 631, 657
BssNI GRCGYC 2 cut(s) 51, 681
BssT1I CCWWGG 2 cut(s) 406, 996
Bst2UI CCWGG 1 cut(s) 1209
Bst4CI ACNGT 2 cut(s) 166, 763
Bst6I CTCTTC 4 cut(s) 109, 874, 980, 1108
BstACI GRCGYC 2 cut(s) 51, 681
BstC8I GCNNGC 4 cut(s) 181, 268, 413, 1260
BstEII GGTNACC 1 cut(s) 110
BstF5I GGATG 3 cut(s) 249, 1057, 1180
BstFNI CGCG 2 cut(s) 512, 689
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 1 cut(s) 683
BstKTI GATC 2 cut(s) 634, 660
BstMAI GTCTC 8 cut(s) 35, 57, 64, 472, 583, 743, 889, 1257
BstMBI GATC 2 cut(s) 631, 657
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 1 cut(s) 1209
BstNSI RCATGY 1 cut(s) 804
BstPI GGTNACC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 1207
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 1276
BstUI CGCG 2 cut(s) 512, 689
BstV1I GCAGC 1 cut(s) 1231
BstV2I GAAGAC 1 cut(s) 960
BsuRI GGCC 3 cut(s) 183, 503, 1274
BtsCI GGATG 3 cut(s) 249, 1057, 1180
BtsIMutI CAGTG 1 cut(s) 1140
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 4 cut(s) 181, 268, 413, 1260
CfoI GCGC 1 cut(s) 683
Cfr10I RCCGGY 2 cut(s) 179, 266
Cfr13I GGNCC 2 cut(s) 1272, 1273
Csp6I GTAC 6 cut(s) 214, 342, 439, 672, 703, 993
CviAII CATG 4 cut(s) 433, 801, 921, 1040
CviQI GTAC 6 cut(s) 214, 342, 439, 672, 703, 993
DinI GGCGCC 1 cut(s) 682
DpnI GATC 2 cut(s) 633, 659
DpnII GATC 2 cut(s) 631, 657
DraI TTTAAA 1 cut(s) 1087
DrdI GACNNNNNNGTC 1 cut(s) 916
DriI GACNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 916
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 4 cut(s) 109, 874, 980, 1108
Eam1105I GACNNNNNGTC 1 cut(s) 50
EarI CTCTTC 4 cut(s) 109, 874, 980, 1108
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 406, 996
Eco24I GRGCYC 1 cut(s) 1276
Eco31I GGTCTC 3 cut(s) 35, 64, 1257
Eco91I GGTNACC 1 cut(s) 110
EcoO65I GGTNACC 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 1207
EcoT14I CCWWGG 2 cut(s) 406, 996
EcoT38I GRGCYC 1 cut(s) 1276
EgeI GGCGCC 1 cut(s) 682
EheI GGCGCC 1 cut(s) 682
ErhI CCWWGG 2 cut(s) 406, 996
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 4 cut(s) 436, 804, 924, 1043
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FatI CATG 4 cut(s) 432, 800, 920, 1039
FauI CCCGC 1 cut(s) 1193
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 1245
FokI GGATG 3 cut(s) 256, 1064, 1167
FriOI GRGCYC 1 cut(s) 1276
Fsp4HI GCNGC 1 cut(s) 1245
FspBI CTAG 2 cut(s) 642, 1010
GlaI GCGC 1 cut(s) 682
GluI GCNGC 1 cut(s) 1245
GsaI CCCAGC 1 cut(s) 1251
GsuI CTGGAG 1 cut(s) 88
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 3 cut(s) 183, 503, 1274
HapII CCGG 2 cut(s) 180, 267
HhaI GCGC 1 cut(s) 683
Hin1I GRCGYC 2 cut(s) 51, 681
Hin1II CATG 4 cut(s) 436, 804, 924, 1043
Hin6I GCGC 1 cut(s) 681
HinP1I GCGC 1 cut(s) 681
HincII GTYRAC 1 cut(s) 1192
HindII GTYRAC 1 cut(s) 1192
HinfI GANTC 4 cut(s) 77, 122, 614, 882
HpaII CCGG 2 cut(s) 180, 267
HphI GGTGA 3 cut(s) 72, 748, 1183
Hpy166II GTNNAC 4 cut(s) 55, 672, 1163, 1192
Hpy188I TCNGA 4 cut(s) 531, 613, 619, 1236
Hpy188III TCNNGA 3 cut(s) 642, 730, 907
Hpy8I GTNNAC 4 cut(s) 55, 672, 1163, 1192
Hpy99I CGWCG 1 cut(s) 1199
HpyAV CCTTC 4 cut(s) 514, 727, 856, 869
HpyCH4III ACNGT 2 cut(s) 166, 763
HpyCH4IV ACGT 2 cut(s) 51, 399
HpyCH4V TGCA 5 cut(s) 491, 718, 775, 827, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpySE526I ACGT 2 cut(s) 51, 399
Hsp92I GRCGYC 2 cut(s) 51, 681
Hsp92II CATG 4 cut(s) 436, 804, 924, 1043
HspAI GCGC 1 cut(s) 681
KasI GGCGCC 1 cut(s) 680
KroI GCCGGC 2 cut(s) 179, 266
KroNI GCCGGC 2 cut(s) 181, 268
Kzo9I GATC 2 cut(s) 631, 657
Lsp1109I GCAGC 1 cut(s) 1231
LweI GCATC 1 cut(s) 1042
MaeI CTAG 2 cut(s) 642, 1010
MaeII ACGT 2 cut(s) 51, 399
MaeIII GTNAC 5 cut(s) 16, 110, 151, 1138, 1171
MalI GATC 2 cut(s) 633, 659
MboI GATC 2 cut(s) 631, 657
MfeI CAATTG 1 cut(s) 497
MhlI GDGCHC 1 cut(s) 1276
MluCI AATT 7 cut(s) 130, 497, 542, 607, 776, 927, 1129
MluI ACGCGT 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 681
MlyI GAGTC 2 cut(s) 608, 891
MmeI TCCRAC 1 cut(s) 22
MroNI GCCGGC 2 cut(s) 179, 266
MseI TTAA 1 cut(s) 1086
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 2 cut(s) 180, 267
MspR9I CCNGG 1 cut(s) 1209
MunI CAATTG 1 cut(s) 497
MvaI CCWGG 1 cut(s) 1209
MvnI CGCG 2 cut(s) 512, 689
MwoI GCNNNNNNNGC 1 cut(s) 509
NaeI GCCGGC 2 cut(s) 181, 268
NarI GGCGCC 1 cut(s) 681
NdeII GATC 2 cut(s) 631, 657
NgoMIV GCCGGC 2 cut(s) 179, 266
NlaIII CATG 4 cut(s) 436, 804, 924, 1043
NlaIV GGNNCC 2 cut(s) 682, 1274
NmeAIII GCCGAG 1 cut(s) 657
NmuCI GTSAC 3 cut(s) 151, 1138, 1171
NspI RCATGY 1 cut(s) 804
PaqCI CACCTGC 1 cut(s) 101
PciI ACATGT 1 cut(s) 800
PcsI WCGNNNNNNNCGW 1 cut(s) 794
PdiI GCCGGC 2 cut(s) 181, 268
PfeI GAWTC 2 cut(s) 77, 122
PfoI TCCNGGA 1 cut(s) 1207
PkrI GCNGC 1 cut(s) 1246
PleI GAGTC 2 cut(s) 608, 890
PluTI GGCGCC 1 cut(s) 684
PpsI GAGTC 2 cut(s) 608, 890
PscI ACATGT 1 cut(s) 800
PsiI TTATAA 1 cut(s) 1128
Psp6I CCWGG 1 cut(s) 1207
PspEI GGTNACC 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 1247
PspGI CCWGG 1 cut(s) 1207
PspN4I GGNNCC 2 cut(s) 682, 1274
PspOMI GGGCCC 1 cut(s) 1272
PspPI GGNCC 2 cut(s) 1272, 1273
RsaI GTAC 6 cut(s) 215, 343, 440, 673, 704, 994
RsaNI GTAC 6 cut(s) 214, 342, 439, 672, 703, 993
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 1086
SatI GCNGC 1 cut(s) 1245
Sau3AI GATC 2 cut(s) 631, 657
Sau96I GGNCC 2 cut(s) 1272, 1273
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 2 cut(s) 608, 891
ScrFI CCNGG 1 cut(s) 1209
SduI GDGCHC 1 cut(s) 1276
SfaNI GCATC 1 cut(s) 1042
SfcI CTRYAG 1 cut(s) 189
SfoI GGCGCC 1 cut(s) 682
SmiMI CAYNNNNRTG 1 cut(s) 383
SpeI ACTAGT 1 cut(s) 1009
Sse9I AATT 7 cut(s) 130, 497, 542, 607, 776, 927, 1129
SsiI CCGC 4 cut(s) 264, 687, 1200, 1256
SspDI GGCGCC 1 cut(s) 680
SspMI CTAG 2 cut(s) 642, 1010
StyD4I CCNGG 1 cut(s) 1207
StyI CCWWGG 2 cut(s) 406, 996
TaaI ACNGT 2 cut(s) 166, 763
TaiI ACGT 2 cut(s) 54, 402
TasI AATT 7 cut(s) 130, 497, 542, 607, 776, 927, 1129
TatI WGTACW 3 cut(s) 213, 341, 438
TfiI GAWTC 2 cut(s) 77, 122
Tru1I TTAA 1 cut(s) 1086
Tru9I TTAA 1 cut(s) 1086
TscAI CASTG 1 cut(s) 1147
TseFI GTSAC 3 cut(s) 151, 1138, 1171
TseI GCWGC 1 cut(s) 1244
Tsp45I GTSAC 3 cut(s) 151, 1138, 1171
TspDTI ATGAA 5 cut(s) 294, 339, 714, 875, 1073
TspGWI ACGGA 1 cut(s) 583
TspRI CASTG 1 cut(s) 1147
XapI RAATTY 2 cut(s) 542, 927
XbaI TCTAGA 1 cut(s) 641
XceI RCATGY 1 cut(s) 804
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 2 cut(s) 642, 1010
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.