AT1G44750

Member of a family of proteins related to PUP1, a purine transporter. May be involved in the transport of purine and purine derivatives such as cytokinins, across the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006723
Physical Location & Seq
Forward (+)
16892527 .. 16896089
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G44750.5

Sequence Viewer

Length: 1140 bp
ATGGCGAAGGAACCAGTTCGTGTTCTTGTTACCGGAGCTGCAGGTAATCAAGAACCAATCTTGGTGAAAGAAGAGAGTGTGGTTGGTATACCAACACCACTCTTGAAGCTTAAAAGTTGGCAATGGTGGGTTCTTGTATCCGTTAACATCTTTTTCCTTATTGGTGGTCAAGCTGCTTCGGTTCTTCTTGGTAGGTTTTACTATGATGAAGGTGGAAACAGTAAATGGATGGCAACTCTTGTTCAAACGGCTGCTTTTCCTATACTCTATATCCCGCTTTTGCTACTTCCGTCTTCGGCTAGTGTAGAGTCTTCGGAGAGTTCGTGTTCACTCAAGTACATTGTTTTGATCTATGTTTTGCTCGGTGTGATCATTGCTGGAGATAATATGTTATACTCTGTTGGACTTTTGTACCTCTCTGCATCGACGTATTCGCTCATTTGCGCTACTCAGTTAGCTTTCAACGCGGTGTTCTCTTATTTCATCAATGCTCAGAAGTTCACTGCTTTGATTCTCAACTCCGTTGTTCTCTTGTCGTTCTCCGCTGCTTTGATAGCTCTCAATGATGATGCGGATACTCCTTCTGGTGTCTCCAGGTCTAAGTATATTGTTGGGTTTGTGTGTACACTTGCTGCGTCTGCTCTCTATTCTCTGTTGCTATCTCTTATGCAGTTCTCGTTCGAGAAGATTCTGAAGAGGGAGACGTTTTCTGTGGTTCTTGAAATGCAAATCTACACTTCTTTAGTGGCGACTTGTGTTTCGGTTATAGGGCTTTTTGCTAGCGGGGAATGGAGAACGCTGCATGGGGAAATGGAAGGTTATCATAAAGGGCAAGCCTCTTATGTACTGACCTTGGTCTGGACAGCAGTTACTTGGCAAGTGTGTTCTGTTGGAGTCGTGGGTTTGATATTTCTGGTGACGTCGCTCTTCTCAAACGTCATTAGTACGCTCTCTCTAGCTGTGACTCCACTAGCAGCTTTGGTTGTGTTCCGTGATAAAATGAGTGGTGTTAAGATTATGGCAATGCTGATCGCTATTTGGGGTTTCGCTTCTTATGTTTACCAGAATCATATTGATGACTTGAAAGTAAGACAAGCACGACAACAAGCTCAAGCCGGGCGGGTAGAACCGCCCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

379

Amino Acids

41.29

Weight (kDa)

7.55

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 1 - 285 1.5e-120 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 923
Acc36I ACCTGC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 88
AccII CGCG 1 cut(s) 467
AciI CCGC 7 cut(s) 275, 467, 543, 572, 783, 1120, 1130
AcuI CTGAAG 1 cut(s) 713
AcyI GRCGYC 1 cut(s) 920
AfaI GTAC 5 cut(s) 338, 413, 625, 846, 946
AfiI CCNNNNNNNGG 1 cut(s) 858
AgsI TTSAA 5 cut(s) 106, 245, 463, 722, 1084
AjnI CCWGG 1 cut(s) 593
AluBI AGCT 8 cut(s) 38, 109, 173, 458, 557, 959, 977, 1109
AluI AGCT 8 cut(s) 38, 109, 173, 458, 557, 959, 977, 1109
Alw26I GTCTC 2 cut(s) 595, 695
ApeKI GCWGC 7 cut(s) 38, 173, 251, 545, 632, 799, 974
ArsI GACNNNNNNTTYG 2 cut(s) 742, 774
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 1117
AsuHPI GGTGA 2 cut(s) 76, 928
AsuNHI GCTAGC 1 cut(s) 779
BaeI ACNNNNGTAYC 2 cut(s) 395, 428
BbsI GAAGAC 2 cut(s) 285, 303
BbvI GCAGC 7 cut(s) 25, 160, 238, 532, 619, 786, 986
BccI CCATC 1 cut(s) 223
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 595
BciVI GTATCC 2 cut(s) 148, 568
BclI TGATCA 1 cut(s) 369
BcnI CCSGG 1 cut(s) 1117
BcoDI GTCTC 2 cut(s) 595, 695
BfaI CTAG 4 cut(s) 300, 780, 956, 971
BfmI CTRYAG 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 32
BfuI GTATCC 2 cut(s) 148, 568
BisI GCNGC 7 cut(s) 39, 174, 252, 546, 633, 800, 975
BlsI GCNGC 7 cut(s) 40, 175, 253, 547, 634, 801, 976
Bme1390I CCNGG 2 cut(s) 595, 1117
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 2 cut(s) 595, 1117
BmsI GCATC 2 cut(s) 431, 559
BmtI GCTAGC 1 cut(s) 783
BoxI GACNNNNGTC 1 cut(s) 854
BpiI GAAGAC 2 cut(s) 285, 303
BpmI CTGGAG 2 cut(s) 399, 577
BpuEI CTTGAG 2 cut(s) 317, 1095
BpuMI CCSGG 1 cut(s) 1117
BsaHI GRCGYC 1 cut(s) 920
BsaJI CCNNGG 1 cut(s) 852
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 858
Bse1I ACTGG 1 cut(s) 14
Bse3DI GCAATG 3 cut(s) 128, 372, 1029
BseBI CCWGG 1 cut(s) 595
BseDI CCNNGG 1 cut(s) 852
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 858
BseMI GCAATG 3 cut(s) 128, 372, 1029
BseMII CTCAG 2 cut(s) 464, 506
BseNI ACTGG 1 cut(s) 14
BseXI GCAGC 7 cut(s) 25, 160, 238, 532, 619, 786, 986
Bsh1236I CGCG 1 cut(s) 467
BsiSI CCGG 2 cut(s) 33, 1116
BslI CCNNNNNNNGG 1 cut(s) 858
BsmAI GTCTC 2 cut(s) 595, 695
BsmBI CGTCTC 1 cut(s) 695
Bsp1407I TGTACA 1 cut(s) 623
Bsp143I GATC 3 cut(s) 348, 369, 1029
BspACI CCGC 7 cut(s) 275, 467, 543, 572, 783, 1120, 1130
BspCNI CTCAG 2 cut(s) 463, 505
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 1 cut(s) 12
BspMAI CTGCAG 1 cut(s) 43
BspMI ACCTGC 1 cut(s) 32
BspOI GCTAGC 1 cut(s) 783
BspQI GCTCTTC 1 cut(s) 932
BsrDI GCAATG 3 cut(s) 128, 372, 1029
BsrGI TGTACA 1 cut(s) 623
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 852
BssMI GATC 3 cut(s) 348, 369, 1029
BssNAI GTATAC 1 cut(s) 89
BssNI GRCGYC 1 cut(s) 920
BssT1I CCWWGG 1 cut(s) 852
Bst1107I GTATAC 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 595
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 3 cut(s) 66, 689, 932
BstACI GRCGYC 1 cut(s) 920
BstAUI TGTACA 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 781, 834
BstDEI CTNAG 3 cut(s) 450, 492, 600
BstF5I GGATG 1 cut(s) 234
BstFNI CGCG 1 cut(s) 467
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 3 cut(s) 351, 372, 1032
BstMAI GTCTC 2 cut(s) 595, 695
BstMBI GATC 3 cut(s) 348, 369, 1029
BstMWI GCNNNNNNNGC 3 cut(s) 464, 554, 638
BstNI CCWGG 1 cut(s) 595
BstPAI GACNNNNGTC 1 cut(s) 854
BstSCI CCNGG 2 cut(s) 593, 1115
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 7 cut(s) 25, 160, 238, 532, 619, 786, 986
BstV2I GAAGAC 2 cut(s) 285, 303
BstZ17I GTATAC 1 cut(s) 89
BsuI GTATCC 2 cut(s) 148, 568
BtsCI GGATG 1 cut(s) 234
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 1 cut(s) 501
BveI ACCTGC 1 cut(s) 32
Cac8I GCNNGC 2 cut(s) 781, 834
CfoI GCGC 1 cut(s) 446
CseI GACGC 1 cut(s) 624
Csp6I GTAC 5 cut(s) 337, 412, 624, 845, 945
CviAII CATG 1 cut(s) 803
CviQI GTAC 5 cut(s) 337, 412, 624, 845, 945
DdeI CTNAG 3 cut(s) 450, 492, 600
DpnI GATC 3 cut(s) 350, 371, 1031
DpnII GATC 3 cut(s) 348, 369, 1029
Eam1104I CTCTTC 3 cut(s) 66, 689, 932
EarI CTCTTC 3 cut(s) 66, 689, 932
Eco130I CCWWGG 1 cut(s) 852
Eco57I CTGAAG 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 593
EcoT14I CCWWGG 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 852
Esp3I CGTCTC 1 cut(s) 695
FaeI CATG 1 cut(s) 806
FatI CATG 1 cut(s) 802
FauI CCCGC 3 cut(s) 282, 776, 1113
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 88
Fnu4HI GCNGC 7 cut(s) 39, 174, 252, 546, 633, 800, 975
FokI GGATG 1 cut(s) 241
Fsp4HI GCNGC 7 cut(s) 39, 174, 252, 546, 633, 800, 975
FspBI CTAG 4 cut(s) 300, 780, 956, 971
GlaI GCGC 1 cut(s) 445
GluI GCNGC 7 cut(s) 39, 174, 252, 546, 633, 800, 975
GsuI CTGGAG 2 cut(s) 399, 577
HapII CCGG 2 cut(s) 33, 1116
HgaI GACGC 1 cut(s) 624
HhaI GCGC 1 cut(s) 446
Hin1I GRCGYC 1 cut(s) 920
Hin1II CATG 1 cut(s) 806
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 6 cut(s) 308, 511, 688, 894, 964, 1066
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 2 cut(s) 33, 1116
HphI GGTGA 2 cut(s) 76, 928
Hpy166II GTNNAC 7 cut(s) 89, 145, 329, 501, 624, 626, 1060
Hpy188I TCNGA 3 cut(s) 316, 495, 693
Hpy188III TCNNGA 5 cut(s) 50, 103, 682, 719, 859
Hpy8I GTNNAC 7 cut(s) 89, 145, 329, 501, 624, 626, 1060
Hpy99I CGWCG 2 cut(s) 430, 925
HpyAV CCTTC 3 cut(s) 203, 591, 809
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 4 cut(s) 428, 704, 920, 936
HpyCH4V TGCA 5 cut(s) 41, 422, 670, 727, 802
HpyF10VI GCNNNNNNNGC 3 cut(s) 464, 554, 638
HpyF3I CTNAG 3 cut(s) 450, 492, 600
HpySE526I ACGT 4 cut(s) 428, 704, 920, 936
Hsp92I GRCGYC 1 cut(s) 920
Hsp92II CATG 1 cut(s) 806
HspAI GCGC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 369
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 3 cut(s) 348, 369, 1029
LguI GCTCTTC 1 cut(s) 932
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 7 cut(s) 25, 160, 238, 532, 619, 786, 986
LweI GCATC 2 cut(s) 431, 559
MaeI CTAG 4 cut(s) 300, 780, 956, 971
MaeII ACGT 4 cut(s) 428, 704, 920, 936
MaeIII GTNAC 4 cut(s) 28, 868, 916, 961
MalI GATC 3 cut(s) 350, 371, 1031
MboI GATC 3 cut(s) 348, 369, 1029
MboII GAAGA 7 cut(s) 83, 176, 285, 303, 697, 706, 919
MlyI GAGTC 3 cut(s) 317, 903, 958
MmeI TCCRAC 2 cut(s) 382, 871
MnlI CCTC 3 cut(s) 425, 690, 847
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 3 cut(s) 111, 144, 1011
MslI CAYNNNNRTG 1 cut(s) 1074
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 2 cut(s) 33, 1116
MspR9I CCNGG 2 cut(s) 595, 1117
MvaI CCWGG 1 cut(s) 595
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 3 cut(s) 464, 554, 638
NciI CCSGG 1 cut(s) 1117
NdeII GATC 3 cut(s) 348, 369, 1029
NheI GCTAGC 1 cut(s) 779
NlaIII CATG 1 cut(s) 806
NlaIV GGNNCC 1 cut(s) 12
NmuCI GTSAC 2 cut(s) 916, 961
PciSI GCTCTTC 1 cut(s) 932
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 3 cut(s) 511, 688, 1066
PkrI GCNGC 7 cut(s) 40, 175, 253, 547, 634, 801, 976
PleI GAGTC 3 cut(s) 316, 902, 958
PpsI GAGTC 3 cut(s) 316, 902, 958
PshAI GACNNNNGTC 1 cut(s) 854
Psp6I CCWGG 1 cut(s) 593
PspGI CCWGG 1 cut(s) 593
PspN4I GGNNCC 1 cut(s) 12
PstI CTGCAG 1 cut(s) 43
RsaI GTAC 5 cut(s) 338, 413, 625, 846, 946
RsaNI GTAC 5 cut(s) 337, 412, 624, 845, 945
RseI CAYNNNNRTG 1 cut(s) 1074
SapI GCTCTTC 1 cut(s) 932
SaqAI TTAA 3 cut(s) 111, 144, 1011
SatI GCNGC 7 cut(s) 39, 174, 252, 546, 633, 800, 975
Sau3AI GATC 3 cut(s) 348, 369, 1029
SchI GAGTC 3 cut(s) 317, 903, 958
ScrFI CCNGG 2 cut(s) 595, 1117
SfaNI GCATC 2 cut(s) 431, 559
SfcI CTRYAG 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 1074
SmlI CTYRAG 2 cut(s) 332, 1110
SmoI CTYRAG 2 cut(s) 332, 1110
SsiI CCGC 7 cut(s) 275, 467, 543, 572, 783, 1120, 1130
SspMI CTAG 4 cut(s) 300, 780, 956, 971
StyD4I CCNGG 2 cut(s) 593, 1115
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 4 cut(s) 431, 707, 923, 939
TaqI TCGA 2 cut(s) 425, 681
TatI WGTACW 3 cut(s) 336, 623, 844
TfiI GAWTC 3 cut(s) 511, 688, 1066
Tru1I TTAA 3 cut(s) 111, 144, 1011
Tru9I TTAA 3 cut(s) 111, 144, 1011
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 2 cut(s) 916, 961
TseI GCWGC 7 cut(s) 38, 173, 251, 545, 632, 799, 974
Tsp45I GTSAC 2 cut(s) 916, 961
TspDTI ATGAA 2 cut(s) 222, 472
TspGWI ACGGA 4 cut(s) 130, 279, 511, 980
TspRI CASTG 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 4 cut(s) 300, 780, 956, 971
ZraI GACGTC 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.