pycom07g07080

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006723
Physical Location & Seq
Reverse (-)
6429082 .. 6432041
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g07080.1

Sequence Viewer

Length: 1209 bp
ATGCACTCTCAAACTATCGCAGGTCTCTTCTTTCCCTCTCGCTCCTCAGTCCTCAAAAGCTCTCTTCTGCTTTTCTCTGGGCCGCGCAGATATCTGATGGGTTCACACGCGGCAGAATCCGATTGGTGGAGAGATAGCCAGGAATCGATTTTACCCAGAGATGGAACTGTGATTGAGCAGACACTATTTGTTAAACTTGGTCGTTGGCAATGGTGGTTTTTGGTGGTACTCAACGTCTTCTTCGTCCTTGTGGGGCAGTCTGCTGCTGTTTTACTGGGAAGATTCTATTATGACCAGGGAGGTAATAGTAAGTGGTTGGCTACTCTTGTACAAACTGCAGCCTTCCCAGTCCTTTTCATCCCGCTTTATTTTCTTCCTTCATCCAAAGATCAAGCAACTTCTTCAAACCTGCCTTCCATTAAAGTTCTTGCCTTGGTTTACTTCTCTATTGGAGTGCTCTTAGCTGGTGACAACATGTTGTATTCTGTCGGACTCTTGTACCTATCTGCCTCTACTTATTCCCTCATATGCGCAACTCAATTAGCTTTTAATGCAGTTTTCTCGTACTTCCTCAACTCTCAGAAGTTCACAATGCTGATTCTTAATTCAGTGATTATCGTGTCATTCTCAGCAGCTCTTATTGCCGTCAATGATGATTCCGGAGGACCAACAGGAGTCTCGAAGGGGAAATATATCCTTGGCTTCATCTGTACCATTGGAGCCTCTGCATTGTACTCTCTTCTGCTTTCCCTCATGCAGCTTTCTTTCCAAAAGGTTTTAAAAAAGGAAACGTTTTCTGTGGTTCTGGAGATGCAAATCTATACGGCTCTGGTCGCTACTTGTGCTGCAACCATAGGTCTTTTTGCAAGTGGGGAATGGAGGAGTTTGAATGGGGAAATGGAAAACTATGGCAAGGGACAAGTTTCTTACGTGATGACCCTAGTTTGGGCAGCTGTGGCTTGGCAAATTTGTTCTGTTGGTGTCGTCGGCTTAATTTTCGTGGTTTCTTCTCTCTTCTCCAATGTAATCAGTACTCTCTCTTTGGCTGTTACTCCTATAGCCGCTGTGATAATCTTCCATGACAAGATGAACGGTGTCAAGGTAATTGCTATGCTTCTGGCTCTTTGGGGTTTCGCCTCTTATATTTATCAGAACTATCTCGATGACTCTAAGGCAAGAAAAAAACTAGCTGATGCTAGGGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

403

Amino Acids

44.07

Weight (kDa)

8.99

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 74 - 391 2.5e-131 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 532
Acc36I ACCTGC 2 cut(s) 11, 417
AccII CGCG 2 cut(s) 85, 110
AccIII TCCGGA 1 cut(s) 659
AciI CCGC 4 cut(s) 83, 110, 362, 1062
AclI AACGTT 1 cut(s) 791
AcsI RAATTY 1 cut(s) 966
AfaI GTAC 7 cut(s) 228, 330, 500, 566, 712, 734, 1033
AfiI CCNNNNNNNGG 4 cut(s) 126, 161, 945, 946
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 2 cut(s) 405, 889
AjnI CCWGG 2 cut(s) 138, 294
AluBI AGCT 7 cut(s) 60, 464, 545, 635, 760, 953, 1190
AluI AGCT 7 cut(s) 60, 464, 545, 635, 760, 953, 1190
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 2 cut(s) 29, 682
Aor13HI TCCGGA 1 cut(s) 659
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 6 cut(s) 263, 338, 632, 757, 845, 950
ApoI RAATTY 1 cut(s) 966
AspLEI GCGC 2 cut(s) 87, 533
AspS9I GGNCC 2 cut(s) 80, 665
AsuHPI GGTGA 1 cut(s) 479
AvaII GGWCC 1 cut(s) 665
BaeI ACNNNNGTAYC 2 cut(s) 482, 515
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 6 cut(s) 250, 350, 644, 769, 832, 962
BccI CCATC 2 cut(s) 91, 155
BceAI ACGGC 2 cut(s) 629, 840
BcgI CGANNNNNNTGC 2 cut(s) 543, 577
BciT130I CCWGG 2 cut(s) 140, 296
BcoDI GTCTC 2 cut(s) 29, 682
BfaI CTAG 3 cut(s) 941, 1187, 1197
BfmI CTRYAG 2 cut(s) 336, 1056
BfuAI ACCTGC 2 cut(s) 11, 417
BisI GCNGC 9 cut(s) 83, 111, 264, 339, 633, 758, 846, 951, 1062
BlsI GCNGC 9 cut(s) 84, 112, 265, 340, 634, 759, 847, 952, 1063
BmcAI AGTACT 1 cut(s) 1033
Bme1390I CCNGG 2 cut(s) 140, 296
Bme18I GGWCC 1 cut(s) 665
BmgT120I GGNCC 2 cut(s) 80, 665
BmiI GGNNCC 2 cut(s) 721, 1203
BmrFI CCNGG 2 cut(s) 140, 296
BmrI ACTGGG 2 cut(s) 284, 341
BmsI GCATC 2 cut(s) 801, 1183
BmuI ACTGGG 2 cut(s) 284, 341
BpiI GAAGAC 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 827
Bsa29I ATCGAT 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 931
BsaBI GATNNNNATC 1 cut(s) 815
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 295, 432, 697
BsaWI WCCGGW 1 cut(s) 659
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 161, 945, 946
Bse1I ACTGG 2 cut(s) 279, 347
Bse3DI GCAATG 1 cut(s) 215
Bse8I GATNNNNATC 1 cut(s) 815
BseAI TCCGGA 1 cut(s) 659
BseBI CCWGG 2 cut(s) 140, 296
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 3 cut(s) 295, 432, 697
BseGI GGATG 2 cut(s) 357, 380
BseJI GATNNNNATC 1 cut(s) 815
BseLI CCNNNNNNNGG 4 cut(s) 126, 161, 945, 946
BseMI GCAATG 1 cut(s) 215
BseMII CTCAG 3 cut(s) 60, 593, 642
BseNI ACTGG 2 cut(s) 279, 347
BseRI GAGGAG 2 cut(s) 34, 895
BseXI GCAGC 6 cut(s) 250, 350, 644, 769, 832, 962
Bsh1236I CGCG 2 cut(s) 85, 110
BshFI GGCC 1 cut(s) 82
BshVI ATCGAT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 459
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 1 cut(s) 930
BslI CCNNNNNNNGG 4 cut(s) 126, 161, 945, 946
BsmAI GTCTC 2 cut(s) 29, 682
BsmFI GGGAC 1 cut(s) 930
BsnI GGCC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 459
Bsp13I TCCGGA 1 cut(s) 659
Bsp1407I TGTACA 1 cut(s) 328
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 4 cut(s) 83, 110, 362, 1062
BspANI GGCC 1 cut(s) 82
BspCNI CTCAG 3 cut(s) 59, 592, 641
BspDI ATCGAT 1 cut(s) 146
BspEI TCCGGA 1 cut(s) 659
BspFNI CGCG 2 cut(s) 85, 110
BspLI GGNNCC 2 cut(s) 721, 1203
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 2 cut(s) 11, 417
BspTNI GGTCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 328
BsrI ACTGG 2 cut(s) 279, 347
BssECI CCNNGG 3 cut(s) 295, 432, 697
BssMI GATC 1 cut(s) 388
BssT1I CCWWGG 2 cut(s) 432, 697
Bst2UI CCWGG 2 cut(s) 140, 296
Bst4CI ACNGT 2 cut(s) 169, 1094
Bst6I CTCTTC 4 cut(s) 32, 69, 744, 1019
BstAUI TGTACA 1 cut(s) 328
BstBAI YACGTR 1 cut(s) 931
BstDEI CTNAG 5 cut(s) 46, 460, 579, 628, 1170
BstF5I GGATG 2 cut(s) 357, 380
BstFNI CGCG 2 cut(s) 85, 110
BstHHI GCGC 2 cut(s) 87, 533
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 2 cut(s) 29, 682
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 5 cut(s) 551, 641, 833, 842, 956
BstNI CCWGG 2 cut(s) 140, 296
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 138, 294
BstSFI CTRYAG 2 cut(s) 336, 1056
BstUI CGCG 2 cut(s) 85, 110
BstV1I GCAGC 6 cut(s) 250, 350, 644, 769, 832, 962
BstV2I GAAGAC 1 cut(s) 229
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 1 cut(s) 82
BsuTUI ATCGAT 1 cut(s) 146
BtsCI GGATG 2 cut(s) 357, 380
BtsIMutI CAGTG 1 cut(s) 615
BveI ACCTGC 2 cut(s) 11, 417
CfoI GCGC 2 cut(s) 87, 533
Cfr13I GGNCC 2 cut(s) 80, 665
ClaI ATCGAT 1 cut(s) 146
Csp6I GTAC 7 cut(s) 227, 329, 499, 565, 711, 733, 1032
CviAII CATG 3 cut(s) 475, 754, 1079
CviQI GTAC 7 cut(s) 227, 329, 499, 565, 711, 733, 1032
DdeI CTNAG 5 cut(s) 46, 460, 579, 628, 1170
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
DraI TTTAAA 1 cut(s) 780
Eam1104I CTCTTC 4 cut(s) 32, 69, 744, 1019
EarI CTCTTC 4 cut(s) 32, 69, 744, 1019
Eco130I CCWWGG 2 cut(s) 432, 697
Eco31I GGTCTC 1 cut(s) 29
Eco32I GATATC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 665
EcoRII CCWGG 2 cut(s) 138, 294
EcoRV GATATC 1 cut(s) 92
EcoT14I CCWWGG 2 cut(s) 432, 697
ErhI CCWWGG 2 cut(s) 432, 697
FaeI CATG 3 cut(s) 478, 757, 1082
FaqI GGGAC 1 cut(s) 930
FatI CATG 3 cut(s) 474, 753, 1078
FauI CCCGC 1 cut(s) 369
FauNDI CATATG 1 cut(s) 527
Fnu4HI GCNGC 9 cut(s) 83, 111, 264, 339, 633, 758, 846, 951, 1062
FokI GGATG 2 cut(s) 344, 367
Fsp4HI GCNGC 9 cut(s) 83, 111, 264, 339, 633, 758, 846, 951, 1062
FspBI CTAG 3 cut(s) 941, 1187, 1197
FspI TGCGCA 1 cut(s) 532
GlaI GCGC 2 cut(s) 86, 532
GluI GCNGC 9 cut(s) 83, 111, 264, 339, 633, 758, 846, 951, 1062
GsuI CTGGAG 1 cut(s) 827
HaeIII GGCC 1 cut(s) 82
HapII CCGG 1 cut(s) 660
HhaI GCGC 2 cut(s) 87, 533
Hin1II CATG 3 cut(s) 478, 757, 1082
Hin6I GCGC 2 cut(s) 85, 531
HinP1I GCGC 2 cut(s) 85, 531
HinfI GANTC 8 cut(s) 116, 143, 282, 492, 598, 656, 675, 1166
HpaII CCGG 1 cut(s) 660
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 3 cut(s) 104, 439, 588
Hpy188I TCNGA 5 cut(s) 96, 121, 491, 582, 1152
Hpy188III TCNNGA 4 cut(s) 660, 679, 806, 1160
Hpy8I GTNNAC 3 cut(s) 104, 439, 588
Hpy99I CGWCG 1 cut(s) 989
HpyAV CCTTC 4 cut(s) 352, 387, 423, 676
HpyCH4III ACNGT 2 cut(s) 169, 1094
HpyCH4IV ACGT 3 cut(s) 234, 791, 930
HpyCH4V TGCA 8 cut(s) 4, 338, 554, 728, 757, 814, 848, 866
HpyF10VI GCNNNNNNNGC 5 cut(s) 551, 641, 833, 842, 956
HpyF3I CTNAG 5 cut(s) 46, 460, 579, 628, 1170
HpySE526I ACGT 3 cut(s) 234, 791, 930
Hsp92II CATG 3 cut(s) 478, 757, 1082
HspAI GCGC 2 cut(s) 85, 531
Kpn2I TCCGGA 1 cut(s) 659
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 2 cut(s) 47, 719
Lsp1109I GCAGC 6 cut(s) 250, 350, 644, 769, 832, 962
LweI GCATC 2 cut(s) 801, 1183
MaeI CTAG 3 cut(s) 941, 1187, 1197
MaeII ACGT 3 cut(s) 234, 791, 930
MaeIII GTNAC 2 cut(s) 467, 1048
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 459
MluCI AATT 5 cut(s) 539, 604, 966, 993, 1104
MlyI GAGTC 3 cut(s) 486, 684, 1160
MmeI TCCRAC 1 cut(s) 469
MroI TCCGGA 1 cut(s) 659
MseI TTAA 6 cut(s) 192, 420, 549, 603, 779, 992
MspA1I CMGCKG 2 cut(s) 953, 1064
MspI CCGG 1 cut(s) 660
MspR9I CCNGG 2 cut(s) 140, 296
MvaI CCWGG 2 cut(s) 140, 296
MvnI CGCG 2 cut(s) 85, 110
MwoI GCNNNNNNNGC 5 cut(s) 551, 641, 833, 842, 956
NdeI CATATG 1 cut(s) 527
NdeII GATC 1 cut(s) 388
NlaIII CATG 3 cut(s) 478, 757, 1082
NlaIV GGNNCC 2 cut(s) 721, 1203
NmuCI GTSAC 1 cut(s) 467
NsbI TGCGCA 1 cut(s) 532
NspI RCATGY 1 cut(s) 478
PciI ACATGT 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 5 cut(s) 116, 143, 282, 598, 656
PkrI GCNGC 9 cut(s) 84, 112, 265, 340, 634, 759, 847, 952, 1063
PleI GAGTC 3 cut(s) 486, 683, 1160
PpsI GAGTC 3 cut(s) 486, 683, 1160
Ppu21I YACGTR 1 cut(s) 931
PscI ACATGT 1 cut(s) 474
Psp1406I AACGTT 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 138, 294
PspGI CCWGG 2 cut(s) 138, 294
PspN4I GGNNCC 2 cut(s) 721, 1203
PspPI GGNCC 2 cut(s) 80, 665
PstI CTGCAG 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 7 cut(s) 228, 330, 500, 566, 712, 734, 1033
RsaNI GTAC 7 cut(s) 227, 329, 499, 565, 711, 733, 1032
SaqAI TTAA 6 cut(s) 192, 420, 549, 603, 779, 992
SatI GCNGC 9 cut(s) 83, 111, 264, 339, 633, 758, 846, 951, 1062
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 2 cut(s) 80, 665
ScaI AGTACT 1 cut(s) 1033
SchI GAGTC 3 cut(s) 486, 684, 1160
ScrFI CCNGG 2 cut(s) 140, 296
SduI GDGCHC 1 cut(s) 459
SfaNI GCATC 2 cut(s) 801, 1183
SfcI CTRYAG 2 cut(s) 336, 1056
SinI GGWCC 1 cut(s) 665
Sse9I AATT 5 cut(s) 539, 604, 966, 993, 1104
SsiI CCGC 4 cut(s) 83, 110, 362, 1062
SspMI CTAG 3 cut(s) 941, 1187, 1197
StyD4I CCNGG 2 cut(s) 138, 294
StyI CCWWGG 2 cut(s) 432, 697
TaaI ACNGT 2 cut(s) 169, 1094
TaiI ACGT 3 cut(s) 237, 794, 933
TaqI TCGA 3 cut(s) 146, 680, 1161
TasI AATT 5 cut(s) 539, 604, 966, 993, 1104
TatI WGTACW 3 cut(s) 328, 732, 1031
TauI GCSGC 3 cut(s) 85, 113, 1064
TfiI GAWTC 5 cut(s) 116, 143, 282, 598, 656
Tru1I TTAA 6 cut(s) 192, 420, 549, 603, 779, 992
Tru9I TTAA 6 cut(s) 192, 420, 549, 603, 779, 992
TscAI CASTG 1 cut(s) 615
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 6 cut(s) 263, 338, 632, 757, 845, 950
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 4 cut(s) 346, 369, 694, 1103
TspRI CASTG 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 1 cut(s) 966
XceI RCATGY 1 cut(s) 478
XspI CTAG 3 cut(s) 941, 1187, 1197
ZrmI AGTACT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.