Rw1G015410

retrograde vesicle-mediated transport, Golgi to ER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006723
Physical Location & Seq
Reverse (-)
34384884 .. 34387359
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015410.1

Sequence Viewer

Length: 1095 bp
ATGCCAAATAGCCAAGAAGCCATCCCATTCAAAAATGAAACTGTGATGGACCCAACACTATTGATCAAACTTGGCCGCTGGCAATGGTGGTTTTTGGTGGCAATCAACATATTCTTCCTCATTGTGGGGCAGGCTGGTGCTGTGTTACTCGGAAGATTTTATTATGACCAGGGTGGTAATAGTACGTGGATGGCTACTCTTGTGCAAACCGCAGCTTTTCCAGTTCTTGTTATCCCACTTTATTTTCTTCCTTCGCAGAAAGAAGATCAATCAACTTCTTCAACTTCACCTTCCCTTCTAGTTCTTGCCTTGATTTACTTCGTTCTTGGACTGGTCATTGCCGGTGACAACATGTTGTATTCTACTGGACTCTTGTACCTATCTGCCTCTACATACTCCATCATTTCTTCAACTCAATTAGCTTTCAATGCAGTCTTCTCATACTTCATCAATTCTCAGAAGTTCAGTGCATTGATTTTCAATTCGGTGATTATCCTGACATTGTCCGTGGCTCTTATTGGTGTGAATGATGACTCGGATGGACCTGCAGGGGTATCAACGCAGAAACATATCATTGGCTTTATCTGTACCCTTGTTGCTTCTGCAATCTACTCCCTTCTACTCTCCCTCATGCAGCTTACTTTCCAGAAGGTTCTGAAAAAGGAAACTTTCTCTGTGGTTTTGCAAATGCAAATTTTTACAGCACTTGTTGCGACTTGTGCTTCAGTTGTGGGTCTTTTTGCAAGTGGGGATTGGAAGACTTTACATGGAGAAATGGAAAGTTTTGGTAAGGGAAGAGTTTCGTATGTGATGACTTTGGTGTGGACAGCTGTTTCTTGGCAAGTTTGTTCTGTTGGTGTGGTGGGTTTAATTTTCGTGGTGTCTTCCCTATTCTCGAACGTAATCAGTACTCTCTCTTTGGCTGTTACTCCTATAGCTGCTGTGATAGTTTTCCATGACAAGATGAATGGTGTGAAGATAATTGCTATGCTGATGGCTATATGGGGTTCTGGTTCTTATATTTATCAAAACTACCTTGATGATGCCAAGGCAAGAAGAGCAGTGGCTGATGTTAGTGAATCGCACATTGCGTGA

Protein Analysis

364

Amino Acids

39.61

Weight (kDa)

6.41

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 32 - 349 3.8e-131 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 553
AciI CCGC 2 cut(s) 76, 210
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 1 cut(s) 693
AcuI CTGAAG 1 cut(s) 708
AfaI GTAC 4 cut(s) 184, 377, 589, 910
AfiI CCNNNNNNNGG 1 cut(s) 124
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 5 cut(s) 31, 282, 411, 427, 481
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 5 cut(s) 215, 422, 637, 830, 938
AluI AGCT 5 cut(s) 215, 422, 637, 830, 938
AlwNI CAGNNNCTG 1 cut(s) 1067
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 3 cut(s) 212, 634, 938
ApoI RAATTY 1 cut(s) 693
Asp700I GAANNNNTTC 1 cut(s) 799
AspS9I GGNCC 2 cut(s) 49, 542
AsuHPI GGTGA 3 cut(s) 279, 356, 499
AvaII GGWCC 2 cut(s) 49, 542
BaeI ACNNNNGTAYC 2 cut(s) 359, 392
BbsI GAAGAC 3 cut(s) 427, 764, 876
BbvI GCAGC 3 cut(s) 224, 646, 925
BccI CCATC 6 cut(s) 29, 40, 184, 407, 533, 988
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 63
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 2 cut(s) 546, 933
BfuAI ACCTGC 1 cut(s) 553
BisI GCNGC 4 cut(s) 76, 213, 635, 939
BlsI GCNGC 4 cut(s) 77, 214, 636, 940
BmcAI AGTACT 1 cut(s) 910
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 2 cut(s) 49, 542
BmgT120I GGNCC 2 cut(s) 49, 542
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 1 cut(s) 170
BmsI GCATC 1 cut(s) 1033
BpiI GAAGAC 3 cut(s) 427, 764, 876
BsaAI YACGTR 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 169, 507, 1047
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 3 cut(s) 221, 336, 370
Bse3DI GCAATG 3 cut(s) 89, 336, 1086
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 3 cut(s) 169, 507, 1047
BseGI GGATG 3 cut(s) 21, 195, 544
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 3 cut(s) 89, 336, 1086
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 3 cut(s) 221, 336, 370
BseXI GCAGC 3 cut(s) 224, 646, 925
BshFI GGCC 1 cut(s) 75
BsiSI CCGG 1 cut(s) 342
BslI CCNNNNNNNGG 1 cut(s) 124
BsnI GGCC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 63, 265
BspACI CCGC 2 cut(s) 76, 210
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 51
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 553
BspQI GCTCTTC 1 cut(s) 1051
BsrDI GCAATG 3 cut(s) 89, 336, 1086
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 3 cut(s) 221, 336, 370
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 169, 507, 1047
BssMI GATC 2 cut(s) 63, 265
BssT1I CCWWGG 1 cut(s) 1047
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 2 cut(s) 790, 1051
BstAPI GCANNNNNTGC 1 cut(s) 710
BstBAI YACGTR 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 80, 132
BstDEI CTNAG 1 cut(s) 456
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 3 cut(s) 21, 195, 544
BstKTI GATC 2 cut(s) 66, 268
BstMBI GATC 2 cut(s) 63, 265
BstMWI GCNNNNNNNGC 4 cut(s) 428, 710, 719, 1058
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 2 cut(s) 546, 933
BstV1I GCAGC 3 cut(s) 224, 646, 925
BstV2I GAAGAC 3 cut(s) 427, 764, 876
BsuRI GGCC 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 3 cut(s) 21, 195, 544
BtsI GCAGTG 1 cut(s) 1068
BtsIMutI CAGTG 2 cut(s) 472, 1068
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 80, 132
CaiI CAGNNNCTG 1 cut(s) 1067
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 2 cut(s) 49, 542
Csp6I GTAC 4 cut(s) 183, 376, 588, 909
CviAII CATG 4 cut(s) 352, 631, 767, 956
CviQI GTAC 4 cut(s) 183, 376, 588, 909
DdeI CTNAG 1 cut(s) 456
DpnI GATC 2 cut(s) 65, 267
DpnII GATC 2 cut(s) 63, 265
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 790, 1051
EarI CTCTTC 2 cut(s) 790, 1051
Eco130I CCWWGG 1 cut(s) 1047
Eco47I GGWCC 2 cut(s) 49, 542
Eco57I CTGAAG 1 cut(s) 708
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 1047
ErhI CCWWGG 1 cut(s) 1047
FaeI CATG 4 cut(s) 355, 634, 770, 959
FatI CATG 4 cut(s) 351, 630, 766, 955
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 4 cut(s) 76, 213, 635, 939
FokI GGATG 3 cut(s) 8, 202, 551
Fsp4HI GCNGC 4 cut(s) 76, 213, 635, 939
FspBI CTAG 1 cut(s) 299
GluI GCNGC 4 cut(s) 76, 213, 635, 939
HaeIII GGCC 1 cut(s) 75
HapII CCGG 1 cut(s) 342
Hin1II CATG 4 cut(s) 355, 634, 770, 959
HinfI GANTC 3 cut(s) 369, 533, 1079
HpaII CCGG 1 cut(s) 342
HphI GGTGA 3 cut(s) 279, 356, 499
Hpy166II GTNNAC 1 cut(s) 825
Hpy188I TCNGA 4 cut(s) 152, 459, 538, 657
Hpy188III TCNNGA 3 cut(s) 496, 646, 895
Hpy8I GTNNAC 1 cut(s) 825
HpyAV CCTTC 5 cut(s) 261, 300, 305, 626, 643
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 185, 900
HpyCH4V TGCA 9 cut(s) 205, 431, 470, 548, 605, 634, 685, 691, 743
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 710, 719, 1058
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 2 cut(s) 185, 900
Hsp92II CATG 4 cut(s) 355, 634, 770, 959
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 2 cut(s) 63, 265
LguI GCTCTTC 1 cut(s) 1051
Lsp1109I GCAGC 3 cut(s) 224, 646, 925
LweI GCATC 1 cut(s) 1033
MaeI CTAG 1 cut(s) 299
MaeII ACGT 2 cut(s) 185, 900
MaeIII GTNAC 3 cut(s) 144, 344, 925
MalI GATC 2 cut(s) 65, 267
MboI GATC 2 cut(s) 63, 265
MluCI AATT 6 cut(s) 416, 451, 481, 693, 870, 981
MlyI GAGTC 2 cut(s) 363, 527
MnlI CCTC 3 cut(s) 128, 397, 638
MroXI GAANNNNTTC 1 cut(s) 799
MseI TTAA 1 cut(s) 869
MspA1I CMGCKG 2 cut(s) 78, 830
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 4 cut(s) 428, 710, 719, 1058
NdeII GATC 2 cut(s) 63, 265
NlaIII CATG 4 cut(s) 355, 634, 770, 959
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 344
NspI RCATGY 1 cut(s) 355
PciI ACATGT 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 1051
PcsI WCGNNNNNNNCGW 1 cut(s) 1088
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 1 cut(s) 1079
PflFI GACNNNGTC 1 cut(s) 502
PkrI GCNGC 4 cut(s) 77, 214, 636, 940
PleI GAGTC 2 cut(s) 363, 527
PpsI GAGTC 2 cut(s) 363, 527
Ppu21I YACGTR 1 cut(s) 186
PscI ACATGT 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 2 cut(s) 49, 542
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 1067
PsyI GACNNNGTC 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 830
RsaI GTAC 4 cut(s) 184, 377, 589, 910
RsaNI GTAC 4 cut(s) 183, 376, 588, 909
SapI GCTCTTC 1 cut(s) 1051
SaqAI TTAA 1 cut(s) 869
SatI GCNGC 4 cut(s) 76, 213, 635, 939
Sau3AI GATC 2 cut(s) 63, 265
Sau96I GGNCC 2 cut(s) 49, 542
SbfI CCTGCAGG 1 cut(s) 550
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 2 cut(s) 363, 527
ScrFI CCNGG 1 cut(s) 170
SdaI CCTGCAGG 1 cut(s) 550
SfaNI GCATC 1 cut(s) 1033
SfcI CTRYAG 2 cut(s) 546, 933
SinI GGWCC 2 cut(s) 49, 542
Sse8387I CCTGCAGG 1 cut(s) 550
Sse9I AATT 6 cut(s) 416, 451, 481, 693, 870, 981
SsiI CCGC 2 cut(s) 76, 210
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 168
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 2 cut(s) 188, 903
TaqI TCGA 1 cut(s) 896
TasI AATT 6 cut(s) 416, 451, 481, 693, 870, 981
TatI WGTACW 1 cut(s) 908
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 1 cut(s) 1079
Tru1I TTAA 1 cut(s) 869
Tru9I TTAA 1 cut(s) 869
TscAI CASTG 2 cut(s) 472, 1068
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 3 cut(s) 212, 634, 938
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 3 cut(s) 51, 436, 980
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 2 cut(s) 472, 1068
Tth111I GACNNNGTC 1 cut(s) 502
VpaK11BI GGWCC 2 cut(s) 49, 542
XapI RAATTY 1 cut(s) 693
XceI RCATGY 1 cut(s) 355
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 1 cut(s) 299
ZrmI AGTACT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.